Lsi03G014930 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G014930
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease T(2)
Location	chr03: 25956090 .. 25956299 (+)
RNA-Seq Expression	Lsi03G014930
Synteny	Lsi03G014930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAGGCCAAAGAGAGAAATTGTGTTCTTCGTGTTTGCCTTAACCATCTTCATTCCTATTGTGAAATCACAAACTTTTGATAATTTTTGGTTGGTTCAACAATGGCCACCAGCAGTTTGTACTTTACAGTCAGGACAATGTATAGGCAAGGGCTACGATACTTCACAATTCATGGTGTGTGGCTTCAAAAGGGAGGAGGCTCTGTAA

mRNA sequence

ATGGCAAGGCCAAAGAGAGAAATTGTGTTCTTCGTGTTTGCCTTAACCATCTTCATTCCTATTGTGAAATCACAAACTTTTGATAATTTTTGGTTGGTTCAACAATGGCCACCAGCAGTTTGTACTTTACAGTCAGGACAATGTATAGGCAAGGGCTACGATACTTCACAATTCATGGTGTGTGGCTTCAAAAGGGAGGAGGCTCTGTAA

Coding sequence (CDS)

ATGGCAAGGCCAAAGAGAGAAATTGTGTTCTTCGTGTTTGCCTTAACCATCTTCATTCCTATTGTGAAATCACAAACTTTTGATAATTTTTGGTTGGTTCAACAATGGCCACCAGCAGTTTGTACTTTACAGTCAGGACAATGTATAGGCAAGGGCTACGATACTTCACAATTCATGGTGTGTGGCTTCAAAAGGGAGGAGGCTCTGTAA

Protein sequence

MARPKREIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDTSQFMVCGFKREEAL

Homology

[Close]

BLAST of Lsi03G014930 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 4.5e-05
Identity = 20/30 (66.67%), Postives = 22/30 (73.33%), Query Frame = 0


Query: 27 FDNFWLVQQWPPAVCTLQ-SGQCIGKGYDT 56
          FD+FW VQQWPPAVC+ Q SG C G G  T
Sbjct: 1  FDSFWFVQQWPPAVCSFQKSGSCPGSGLRT 30

[Close]

BLAST of Lsi03G014930 vs. ExPASy TrEMBL
Match: A0A1S3B8Q3 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 9.2e-17
Identity = 42/62 (67.74%), Postives = 50/62 (80.65%), Query Frame = 0


Query: 1  MARPKREIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDTSQFMV 60
          M + KR++VFFVF LTI  PIVKSQTFDNFW VQQWPPA+CTLQSG+C+G+G  T  F +
Sbjct: 1  MEKTKRDVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRG--TRSFTI 60

Query: 61 CG 63
           G
Sbjct: 61 HG 60

[Close]

BLAST of Lsi03G014930 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.9e-14
Identity = 39/56 (69.64%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 7  EIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDTSQFMVCG 63
          ++VFFVF LTI  PIVKSQTFD+FW VQQWPPAVCTLQSG+C+G+G  T  F + G
Sbjct: 8  DVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRG--TRSFTIHG 61

[Close]

BLAST of Lsi03G014930 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.2e-13
Identity = 38/56 (67.86%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 1  MARPKREIVF-FVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDT 56
          M   +REIVF FV A+TIF+PIVKSQTFD+FW VQQWPPAVC+ Q G+C+G+G  T
Sbjct: 1  MKTSRREIVFMFVLAITIFLPIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRT 56

[Close]

BLAST of Lsi03G014930 vs. ExPASy TrEMBL
Match: Q40115 (Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 8.9e-12
Identity = 35/52 (67.31%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 1  MARPKREIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKG 53
          MA  KREIV  VF LTI  P+VKSQTFD+FW+VQ WPPAVC+ Q G+C+G+G
Sbjct: 1  MAMAKREIV-LVFVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRCVGQG 51

[Close]

BLAST of Lsi03G014930 vs. ExPASy TrEMBL
Match: A0A6J1CKH7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.2e-11
Identity = 34/56 (60.71%), Postives = 40/56 (71.43%), Query Frame = 0


Query: 1  MARPKREIV-FFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDT 56
          M   KRE+V  F   +TIF P+VKSQ FDNFW VQQWPPAVC  Q G+C+G+G  T
Sbjct: 5  MVISKREVVLMFALTITIFFPVVKSQAFDNFWFVQQWPPAVCNFQQGRCVGQGLRT 60

[Close]

BLAST of Lsi03G014930 vs. NCBI nr
Match: XP_008443402.1 (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-16
Identity = 42/62 (67.74%), Postives = 50/62 (80.65%), Query Frame = 0


Query: 1  MARPKREIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDTSQFMV 60
          M + KR++VFFVF LTI  PIVKSQTFDNFW VQQWPPA+CTLQSG+C+G+G  T  F +
Sbjct: 1  MEKTKRDVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRG--TRSFTI 60

Query: 61 CG 63
           G
Sbjct: 61 HG 60

[Close]

BLAST of Lsi03G014930 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 87.8 bits (216), Expect = 4.0e-14
Identity = 39/56 (69.64%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 7  EIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDTSQFMVCG 63
          ++VFFVF LTI  PIVKSQTFD+FW VQQWPPAVCTLQSG+C+G+G  T  F + G
Sbjct: 8  DVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRG--TRSFTIHG 61

[Close]

BLAST of Lsi03G014930 vs. NCBI nr
Match: XP_038905534.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 86.3 bits (212), Expect = 1.1e-13
Identity = 40/62 (64.52%), Postives = 46/62 (74.19%), Query Frame = 0


Query: 1  MARPKREIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDTSQFMV 60
          MA  KR IV  VF LTI  PIV+SQTFD+FW VQQWPPAVC LQSG+C+G+G  T  F +
Sbjct: 1  MATTKRNIVLLVFGLTILFPIVQSQTFDDFWFVQQWPPAVCALQSGRCVGRG--TRSFTI 60

Query: 61 CG 63
           G
Sbjct: 61 HG 60

[Close]

BLAST of Lsi03G014930 vs. NCBI nr
Match: XP_022155091.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 85.1 bits (209), Expect = 2.6e-13
Identity = 38/56 (67.86%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 1  MARPKREIVF-FVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKGYDT 56
          M   +REIVF FV A+TIF+PIVKSQTFD+FW VQQWPPAVC+ Q G+C+G+G  T
Sbjct: 1  MKTSRREIVFMFVLAITIFLPIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRT 56

[Close]

BLAST of Lsi03G014930 vs. NCBI nr
Match: BAA10891.1 (ribonuclease (RNase LC1) [Luffa aegyptiaca])

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-11
Identity = 35/52 (67.31%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 1  MARPKREIVFFVFALTIFIPIVKSQTFDNFWLVQQWPPAVCTLQSGQCIGKG 53
          MA  KREIV  VF LTI  P+VKSQTFD+FW+VQ WPPAVC+ Q G+C+G+G
Sbjct: 1  MAMAKREIV-LVFVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRCVGQG 51

The following BLAST results are available for this feature:

BLAST of Lsi03G014930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	4.5e-05	66.67	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]

BLAST of Lsi03G014930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B8Q3	9.2e-17	67.74	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1	[more]
A0A0A0LC37	1.9e-14	69.64	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A6J1DQN7	1.2e-13	67.86	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1	[more]
Q40115	8.9e-12	67.31	Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1CKH7	1.2e-11	60.71	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1	[more]

BLAST of Lsi03G014930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008443402.1	1.9e-16	67.74	PREDICTED: ribonuclease MC-like [Cucumis melo]	[more]
XP_004147499.1	4.0e-14	69.64	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]
XP_038905534.1	1.1e-13	64.52	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022155091.1	2.6e-13	67.86	ribonuclease MC-like [Momordica charantia]	[more]
BAA10891.1	1.8e-11	67.31	ribonuclease (RNase LC1) [Luffa aegyptiaca]	[more]

BLAST of Lsi03G014930 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 9..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014930.1	Lsi03G014930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding