Lsi03G014870 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G014870
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	ribonuclease MC-like
Location	chr03: 25911950 .. 25913318 (-)
RNA-Seq Expression	Lsi03G014870
Synteny	Lsi03G014870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTACTTGCGAGTTGTGTTTCAATGGCAGCCAGCCACTTGCGGTGATGCGACCAAGCCTTATCCTTATTGCAATCAAGCTCCACAAAATAGATTCAGTATCCATGGGATGTGGCCTAGCCTATACAATGGTCAAACCCAATGTTCTGGTAGTTCCTTCGATATAAACCAGGTAATTACATTATCACAATTATTATTTGTGAATCATAAATACAAGTTTGGAGAGTTGTGAACTCCTGAGGCTTACAGTGTATAAAGAATTAATAAGACTTAAAATTGATGTTTTTTTAGGCCTACAATGTATAACCGATGAGTTATACTCATATTGATACATCATATTAATTTTTGTAAACAAATTTTAACAATATTTTGACATATAGGCCCAATAGATCAATTCTTATAAACATGAACATTAAATTTCAACATTTTAGAAGATCAAAAATGAAATTGAAAAGTATATTATTATTATTTTTTTAGTTAAGCTATGCAGGGATTATCATTAATCAAATCTCAAACTGCTTTTAGATCCAAGTAATAGTGCCTTAACGAATTAAACTATATTCATGTTGACAATCGAAAATTACCAACGACCAGTTAGTTTTTAAATAAACACTACTTCCGACTATAATTTCTTTAATTTGTTATCTAGTTACGTAGGAATATATCATTTTAAGAAAACCAAACCAACTTTTGATAACTTAAAAAAAGTAGTATTTTTAAAAACGTGTTTTTTTAAATAAAAAATTTAGCTAAAAATTCAAGTATTCAATTAAGAAACATGAAAATTCTTTGTAAGAAAACAAACATAATTTTAAAAAATCAAAAATAAAAAAATCAAACGGTTATCAAATAGAGTCTATCCAATTATGAATGATGTTTCGGTTTTTCTAACCCTAAACCATTCCATTATAACTCTCTACATATTACTTTGTCTAGGTTAAGGTTCTAGAACCCAGACTGAGTGAAATGTGGGGAAACATTATCACAGGAGACAACCAATGGCTATGGAAACACGAATGGGATAAACATGGAACTTGCATAGAAGACATGTTATTAGATCAATCAGGATATTTTGAATATGCAATGAACACACTCTATTATTTCAATCCTGAGGAAATTCTAAAGAACAGTGGAATTTGGCCTACTCAAGAAGGATACTACACATTGGATGAAATTAAGAATGCAATCAGAGCAAAAACTGGAAAATTACCAGCCATTAGCTGCAATTACGATCATTGGACTTCATATGAACAATTGCATGAAATAAGCTACTGTACTACTCGGGATGGTTCAACTTTTGTGGATTGTCCCTCCAATGCCAACAAATGCAATGGTTATTCTGCCATGTGGTATCCCTCTCCCTACTAG

mRNA sequence

ATGACTACTTGCGAGTTGTGTTTCAATGGCAGCCAGCCACTTGCGGTGATGCGACCAAGCCTTATCCTTATTGCAATCAAGCTCCACAAAATAGATTCAGTATCCATGGGATGTGGCCTAGCCTATACAATGGTCAAACCCAATGTTCTGGTTAAGGTTCTAGAACCCAGACTGAGTGAAATGTGGGGAAACATTATCACAGGAGACAACCAATGGCTATGGAAACACGAATGGGATAAACATGGAACTTGCATAGAAGACATGTTATTAGATCAATCAGGATATTTTGAATATGCAATGAACACACTCTATTATTTCAATCCTGAGGAAATTCTAAAGAACAGTGGAATTTGGCCTACTCAAGAAGGATACTACACATTGGATGAAATTAAGAATGCAATCAGAGCAAAAACTGGAAAATTACCAGCCATTAGCTGCAATTACGATCATTGGACTTCATATGAACAATTGCATGAAATAAGCTACTGTACTACTCGGGATGGTTCAACTTTTGTGGATTGTCCCTCCAATGCCAACAAATGCAATGGTTATTCTGCCATGTGGTATCCCTCTCCCTACTAG

Coding sequence (CDS)

ATGACTACTTGCGAGTTGTGTTTCAATGGCAGCCAGCCACTTGCGGTGATGCGACCAAGCCTTATCCTTATTGCAATCAAGCTCCACAAAATAGATTCAGTATCCATGGGATGTGGCCTAGCCTATACAATGGTCAAACCCAATGTTCTGGTTAAGGTTCTAGAACCCAGACTGAGTGAAATGTGGGGAAACATTATCACAGGAGACAACCAATGGCTATGGAAACACGAATGGGATAAACATGGAACTTGCATAGAAGACATGTTATTAGATCAATCAGGATATTTTGAATATGCAATGAACACACTCTATTATTTCAATCCTGAGGAAATTCTAAAGAACAGTGGAATTTGGCCTACTCAAGAAGGATACTACACATTGGATGAAATTAAGAATGCAATCAGAGCAAAAACTGGAAAATTACCAGCCATTAGCTGCAATTACGATCATTGGACTTCATATGAACAATTGCATGAAATAAGCTACTGTACTACTCGGGATGGTTCAACTTTTGTGGATTGTCCCTCCAATGCCAACAAATGCAATGGTTATTCTGCCATGTGGTATCCCTCTCCCTACTAG

Protein sequence

MTTCELCFNGSQPLAVMRPSLILIAIKLHKIDSVSMGCGLAYTMVKPNVLVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMWYPSPY

Homology

[Close]

BLAST of Lsi03G014870 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 7.2e-16
Identity = 40/109 (36.70%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 67  TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYT 126
           +G     W HEW+KHGTC E +L +Q  YF+ A++     +   IL+ + I P  E  Y 
Sbjct: 108 SGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGES-YD 167

Query: 127 LDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP 176
           L  I+NAI++  G  P I CN D  +   QL+++  C    GS+ ++CP
Sbjct: 168 LVNIRNAIKSAIGYTPWIQCNVDQ-SGNSQLYQVYICVDGSGSSLIECP 214

[Close]

BLAST of Lsi03G014870 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.6e-15
Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 0


Query: 54  LEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILK 113
           L+ +L+ +W N++  +NQ  W HEW KHGTC E    +Q+ YF+ A++    ++    L+
Sbjct: 62  LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSES-TFNQAAYFKLAVDMRNNYDIIGALR 121

Query: 114 NSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVD 173
                P      +   IK  ++AK GK P + C  D  T    L ++  C  +DGST +D
Sbjct: 122 PHAAGPNGR-TKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 174 C 175
           C
Sbjct: 182 C 180

[Close]

BLAST of Lsi03G014870 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 8.0e-15
Identity = 46/127 (36.22%), Postives = 64/127 (50.39%), Query Frame = 0


Query: 67  TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYT 126
           + D    W HEW KHGTC     L+Q  YF+ A++     N  + L N+GI P    YY 
Sbjct: 107 SSDGLKFWSHEWLKHGTC---SALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYG 166

Query: 127 LDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP--SNANKCNGY 186
           ++ IK AI    G  P I CN D   ++ QL+++  C     S F+DCP   +  KC   
Sbjct: 167 VESIKKAIEKGVGHTPFIECNVDSQGNH-QLYQVYLCVDSSASKFIDCPIFPHGGKCG-- 226

Query: 187 SAMWYPS 192
           S + +PS
Sbjct: 227 SKIEFPS 227

[Close]

BLAST of Lsi03G014870 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-14
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 0


Query: 67  TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYT 126
           +G  +  W+HEW+KHGTC E  ++DQ  YF+ A+N     N    L  +GI P  +  Y+
Sbjct: 109 SGSGEAFWEHEWEKHGTCSES-VIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKS-YS 168

Query: 127 LDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP 176
           L+ I+++I+   G  P + CN D  +   QL+++  C  R GS  ++CP
Sbjct: 169 LESIRDSIKESIGFTPWVECNRD-GSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of Lsi03G014870 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-14
Identity = 41/109 (37.61%), Postives = 56/109 (51.38%), Query Frame = 0


Query: 67  TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYT 126
           + D    W HEW+KHGTC E   LDQ  YFE  +      N    L N+GI P  + +Y 
Sbjct: 101 SNDGMKFWTHEWEKHGTCAESE-LDQHDYFEAGLKLKQKANLLHALTNAGIKP-DDKFYE 160

Query: 127 LDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP 176
           + +I+N I+   G  P I CN D  +   QL++I  C     S F++CP
Sbjct: 161 MKDIENTIKQVVGFAPGIECNKDS-SHNSQLYQIYLCVDTSASKFINCP 206

[Close]

BLAST of Lsi03G014870 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.4e-38
Identity = 73/139 (52.52%), Postives = 96/139 (69.06%), Query Frame = 0


Query: 51  VKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEE 110
           + +L+P +SE+W NII G+N WLW HEWDKHGTC E++L DQ  YF +AM+T   +N   
Sbjct: 84  ISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEEILFDQDRYFSFAMDTYIDYNLLV 143

Query: 111 ILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGST 170
           +L NS I P  +  Y+ D++  AIRAKTGK PA+ CNY+ WT  +Q+HE+S C   D S 
Sbjct: 144 LLGNSQITPNGQ-IYSRDQVYAAIRAKTGKTPAVRCNYNRWTGEQQMHEVSLCYNHDASA 203

Query: 171 FVDCPSNANKCNGYSAMWY 190
            VDCP NANKC+ Y  +WY
Sbjct: 204 VVDCPLNANKCDNY-FVWY 220

[Close]

BLAST of Lsi03G014870 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.6e-34
Identity = 72/135 (53.33%), Postives = 88/135 (65.19%), Query Frame = 0


Query: 54  LEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILK 113
           L+P +SE+W N+ TGDN WLW+HEWDKHGTC E    DQ  YF + M+   + N + IL 
Sbjct: 86  LQPGISEIWPNVATGDNFWLWQHEWDKHGTCSESTFYDQQEYFGFGMDAYIHNNLQVILS 145

Query: 114 NSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVD 173
           N+ I P     YT D++  AIRAKTGK PA+ CNY+  T   QLHEIS C  +D S F D
Sbjct: 146 NNQITPNGR-TYTRDQVYAAIRAKTGKTPAVRCNYNSRTGASQLHEISLCFKKDASVFED 205

Query: 174 CPSNANKCNGYSAMW 189
           CP NANKC G S +W
Sbjct: 206 CPLNANKC-GASFVW 218

[Close]

BLAST of Lsi03G014870 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 3.1e-22
Identity = 53/135 (39.26%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 50  LVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPE 109
           ++  ++ RL + W N+ITG N   W +EW KHGTC     L+Q  YF + +     ++ +
Sbjct: 83  MILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTC-SSAYLNQFHYFNFGLWVYTNYDLQ 142

Query: 110 EILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGS 169
            IL   GI+P  +  YT   ++NAI + TGK PA+ CN ++ T   QLHEIS C +++G 
Sbjct: 143 SILYQYGIYPNGQ-QYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQ 202

Query: 170 TFVDCPSNANKCNGY 185
             +DCP N   CN Y
Sbjct: 203 ILIDCPFNVVTCNEY 215

[Close]

BLAST of Lsi03G014870 vs. ExPASy TrEMBL
Match: A0A175YIT6 (RING-type domain-containing protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_031018 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 3.8e-20
Identity = 55/140 (39.29%), Postives = 77/140 (55.00%), Query Frame = 0


Query: 54  LEPRLSEMWGNII--TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEI 113
           LE RL + W ++   + D    W HEWDKHGTC E   LDQ  YF+ A++     N  + 
Sbjct: 460 LESRLEKDWPSLACPSSDGMRFWGHEWDKHGTCSES-FLDQHSYFKAALDLKNKANLLQA 519

Query: 114 LKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTF 173
           L N+GI P    YY+L+ IK+AI+  TG  P I CN D    Y QL+++  C    GS F
Sbjct: 520 LNNAGIKPADGKYYSLESIKDAIKEATGYTPYIDCNVDASRKYHQLYQVYMCVEASGSEF 579

Query: 174 VDCPSNANKCNGYSAMWYPS 192
           ++CP   +  +  S + +PS
Sbjct: 580 INCPLLPHGRSCTSQVEFPS 598

[Close]

BLAST of Lsi03G014870 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 8.5e-20
Identity = 52/140 (37.14%), Postives = 71/140 (50.71%), Query Frame = 0


Query: 51  VKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEE 110
           +  +  RL+  W ++I G N  LW HEW  HGTC     L Q  YF + ++T   ++   
Sbjct: 80  ISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYPS-LSQLDYFTFGLDTYAKYDIYS 139

Query: 111 ILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGST 170
            L    I+P+ E  Y+   I NAIR  T KLP + CN + ++   QLHEI  C  +    
Sbjct: 140 ALDKEDIYPS-EDVYSKSSINNAIRKITQKLPGLRCNTNDYSRERQLHEIILCFAKSNRA 199

Query: 171 FVDCPSNANKCNGYSAMWYP 191
            +DCPSN   C  Y   WYP
Sbjct: 200 IIDCPSNVVTCGNY-VYWYP 216

[Close]

BLAST of Lsi03G014870 vs. NCBI nr
Match: XP_038904516.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 293.1 bits (749), Expect = 1.7e-75
Identity = 123/148 (83.11%), Postives = 136/148 (91.89%), Query Frame = 0


Query: 46  KPNVLVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYY 105
           + N  VKVLEPRL+EMWGN+ITGDNQWLWK EWDKHGTCIED+L DQSGYFEY MNTLYY
Sbjct: 90  RENYKVKVLEPRLNEMWGNVITGDNQWLWKPEWDKHGTCIEDILFDQSGYFEYTMNTLYY 149

Query: 106 FNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTT 165
           FNPEEILKN GIWPTQEGYYTLDEIK+ I+A+TGKLPA+ CNY+HWTSY+QLHEISYC T
Sbjct: 150 FNPEEILKNGGIWPTQEGYYTLDEIKSVIKARTGKLPAVGCNYNHWTSYQQLHEISYCVT 209

Query: 166 RDGSTFVDCPSNANKCNGYSAMWYPSPY 194
           ++G TFVDCPSNANKCNG+SAMWYP PY
Sbjct: 210 KEGQTFVDCPSNANKCNGHSAMWYPPPY 237

[Close]

BLAST of Lsi03G014870 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 168.7 bits (426), Expect = 4.9e-38
Identity = 73/139 (52.52%), Postives = 96/139 (69.06%), Query Frame = 0


Query: 51  VKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEE 110
           + +L+P +SE+W NII G+N WLW HEWDKHGTC E++L DQ  YF +AM+T   +N   
Sbjct: 84  ISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEEILFDQDRYFSFAMDTYIDYNLLV 143

Query: 111 ILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGST 170
           +L NS I P  +  Y+ D++  AIRAKTGK PA+ CNY+ WT  +Q+HE+S C   D S 
Sbjct: 144 LLGNSQITPNGQ-IYSRDQVYAAIRAKTGKTPAVRCNYNRWTGEQQMHEVSLCYNHDASA 203

Query: 171 FVDCPSNANKCNGYSAMWY 190
            VDCP NANKC+ Y  +WY
Sbjct: 204 VVDCPLNANKCDNY-FVWY 220

[Close]

BLAST of Lsi03G014870 vs. NCBI nr
Match: XP_038903744.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 158.3 bits (399), Expect = 6.7e-35
Identity = 70/140 (50.00%), Postives = 92/140 (65.71%), Query Frame = 0


Query: 51  VKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEE 110
           + +L+P +SE+W N++TG NQWLW HEWDKHGTC E +L DQ  YF + M+     N   
Sbjct: 85  ISILQPGISEIWPNVVTGKNQWLWGHEWDKHGTCTESILFDQEEYFSFTMDKYIENNLSF 144

Query: 111 ILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGST 170
           IL  S I P  +  Y+  +I  AI AKTGK PA+ CNY++W+  EQLHEI+ C   + + 
Sbjct: 145 ILGKSQITPNGQ-LYSRAQIYAAISAKTGKTPAVRCNYNYWSGVEQLHEITLCYNWNATA 204

Query: 171 FVDCPSNANKCNGYSAMWYP 191
           F DCP NANKC+  + MWYP
Sbjct: 205 FEDCPLNANKCDN-TFMWYP 222

[Close]

BLAST of Lsi03G014870 vs. NCBI nr
Match: XP_022149793.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 156.0 bits (393), Expect = 3.3e-34
Identity = 72/135 (53.33%), Postives = 88/135 (65.19%), Query Frame = 0


Query: 54  LEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILK 113
           L+P +SE+W N+ TGDN WLW+HEWDKHGTC E    DQ  YF + M+   + N + IL 
Sbjct: 86  LQPGISEIWPNVATGDNFWLWQHEWDKHGTCSESTFYDQQEYFGFGMDAYIHNNLQVILS 145

Query: 114 NSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVD 173
           N+ I P     YT D++  AIRAKTGK PA+ CNY+  T   QLHEIS C  +D S F D
Sbjct: 146 NNQITPNGR-TYTRDQVYAAIRAKTGKTPAVRCNYNSRTGASQLHEISLCFKKDASVFED 205

Query: 174 CPSNANKCNGYSAMW 189
           CP NANKC G S +W
Sbjct: 206 CPLNANKC-GASFVW 218

[Close]

BLAST of Lsi03G014870 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 115.2 bits (287), Expect = 6.5e-22
Identity = 53/135 (39.26%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 50  LVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPE 109
           ++  ++ RL + W N+ITG N   W +EW KHGTC     L+Q  YF + +     ++ +
Sbjct: 83  MILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTC-SSAYLNQFHYFNFGLWVYTNYDLQ 142

Query: 110 EILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGS 169
            IL   GI+P  +  YT   ++NAI + TGK PA+ CN ++ T   QLHEIS C +++G 
Sbjct: 143 SILYQYGIYPNGQ-QYTKTVVQNAIYSATGKWPALRCNNNYRTGNMQLHEISLCFSKNGQ 202

Query: 170 TFVDCPSNANKCNGY 185
             +DCP N   CN Y
Sbjct: 203 ILIDCPFNVVTCNEY 215

[Close]

BLAST of Lsi03G014870 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-20
Identity = 51/120 (42.50%), Postives = 69/120 (57.50%), Query Frame = 0


Query: 58  LSEMWGNIITGDNQW--LWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNS 117
           L + W  +    N+    W+HEW+KHGTC E  ++DQ  YFE A+      N  +ILKNS
Sbjct: 96  LKKNWPTLSCPSNEGFNFWEHEWEKHGTCSES-VMDQHEYFENALKLKQKANLLQILKNS 155

Query: 118 GIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP 176
           GI P  +G+Y LD+I NAI+   G  P I CN D   +  QLH+I  C    G+ F++CP
Sbjct: 156 GINP-DDGFYNLDKITNAIKDGIGFTPGIECNKDPERN-AQLHQIYICVDTSGTEFIECP 212

[Close]

BLAST of Lsi03G014870 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-17
Identity = 51/135 (37.78%), Postives = 73/135 (54.07%), Query Frame = 0


Query: 42  YTMVKPNVLVKVLEPRLSEMWGNIITGDNQW-LWKHEWDKHGTCIEDMLLDQSGYFEYAM 101
           + + K + LV  +E + +E WG      N+  LW+HEW+KHGTC++  + DQ  YF   +
Sbjct: 82  FDISKISDLVCQMEKKWTE-WGVWACPSNETNLWEHEWNKHGTCVQS-IFDQHSYFRTNL 141

Query: 102 NTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEI 161
              +  +   IL   GI P  +G+Y+LDEIKNAI+   G  P I CN D     +QL +I
Sbjct: 142 KFKHKVHLLNILIQKGIKP-NDGFYSLDEIKNAIKCAIGFAPGIECNED-VKGNKQLFQI 201

Query: 162 SYCTTRDGSTFVDCP 176
             C       FV+CP
Sbjct: 202 YICLDNYAKEFVECP 212

[Close]

BLAST of Lsi03G014870 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 80.9 bits (198), Expect = 1.3e-15
Identity = 41/109 (37.61%), Postives = 56/109 (51.38%), Query Frame = 0


Query: 67  TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYT 126
           + D    W HEW+KHGTC E   LDQ  YFE  +      N    L N+GI P  + +Y 
Sbjct: 101 SNDGMKFWTHEWEKHGTCAESE-LDQHDYFEAGLKLKQKANLLHALTNAGIKP-DDKFYE 160

Query: 127 LDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP 176
           + +I+N I+   G  P I CN D  +   QL++I  C     S F++CP
Sbjct: 161 MKDIENTIKQVVGFAPGIECNKDS-SHNSQLYQIYLCVDTSASKFINCP 206

[Close]

BLAST of Lsi03G014870 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 80.9 bits (198), Expect = 1.3e-15
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 0


Query: 67  TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYT 126
           +G  +  W+HEW+KHGTC E  ++DQ  YF+ A+N     N    L  +GI P  +  Y+
Sbjct: 109 SGSGEAFWEHEWEKHGTCSES-VIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKS-YS 168

Query: 127 LDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP 176
           L+ I+++I+   G  P + CN D  +   QL+++  C  R GS  ++CP
Sbjct: 169 LESIRDSIKESIGFTPWVECNRD-GSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of Lsi03G014870 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 53.1 bits (126), Expect = 2.8e-07
Identity = 28/94 (29.79%), Postives = 40/94 (42.55%), Query Frame = 0


Query: 74  WKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNA 133
           W HEW+KHGTC   +  D+  YF   +N     N  ++L  +G   +    Y L  I  A
Sbjct: 126 WGHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTA 185

Query: 134 IRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRD 168
           I+      P + C  D       + EI  C  +D
Sbjct: 186 IQNAFHITPEVVCKRD------AIDEIRICFYKD 213

The following BLAST results are available for this feature:

BLAST of Lsi03G014870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P80022	7.2e-16	36.70	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P23540	1.6e-15	33.06	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80196	8.0e-15	36.22	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P42813	1.8e-14	35.78	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	1.8e-14	37.61	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]

BLAST of Lsi03G014870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7NBR4	2.4e-38	52.52	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1D7Q5	1.6e-34	53.33	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]
A0A6J1E0X1	3.1e-22	39.26	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A175YIT6	3.8e-20	39.29	RING-type domain-containing protein OS=Daucus carota subsp. sativus OX=79200 GN=...	[more]
A0A6J1E266	8.5e-20	37.14	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]

BLAST of Lsi03G014870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904516.1	1.7e-75	83.11	ribonuclease MC-like [Benincasa hispida]	[more]
AEM42996.1	4.9e-38	52.52	S-RNase [Siraitia grosvenorii]	[more]
XP_038903744.1	6.7e-35	50.00	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022149793.1	3.3e-34	53.33	ribonuclease MC-like [Momordica charantia]	[more]
XP_022158939.1	6.5e-22	39.26	ribonuclease MC-like [Momordica charantia]	[more]

BLAST of Lsi03G014870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	1.3e-20	42.50	Ribonuclease T2 family protein	[more]
AT1G14210.1	1.0e-17	37.78	Ribonuclease T2 family protein	[more]
AT1G26820.1	1.3e-15	37.61	ribonuclease 3	[more]
AT2G02990.1	1.3e-15	35.78	ribonuclease 1	[more]
AT2G39780.1	2.8e-07	29.79	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 52..174 e-value: 2.2E-30 score: 106.2
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 51..185
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 27..192 e-value: 1.1E-34 score: 122.2
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 51..184
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 73..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014870.1	Lsi03G014870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding