Lsi03G014820 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G014820
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	aquaporin NIP2-1-like
Location	chr03: 25812518 .. 25818290 (-)
RNA-Seq Expression	Lsi03G014820
Synteny	Lsi03G014820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCATGCCTCAGCTTCATTATAAAAAGGGTAGCTTGCAGCTTCAAGTTGATGTGCAAATAAGTTTTTTATAATAATATTAGCGTTTACGTTGAAACAGAGGGAGAGAATATTTTCAGTGTTCTTCCCCTTTGGCATTGGTGAATAATATTTGCCAAAGCTTTAGAGATCTAAACCACTTATAAAAGAAGTGCTTCCACTTCTTTGTATTTCGTTCCAACACAAGAAAGAAAAAAAATGAGTTCTGTTCAAAATCCTGAGCTCCCCAACCAAGAAGCTGTGGTCAATGTTAACGAATTCGTGTCTGTTGAAAACCCTGATTCCAAACGCTCCAAGTTTGGATCTTTCTTCAAAAACCCTTACCCTCCTGGGTTTTCTCGAAAGGTACCCTTCCTTAAACTCATCAAAACCTTGTCTTTCTCTTCTGTTTTCTTTTCTTTTTTTCTTTCCCTCGAAAAATTCGTTTTCTTGAGTAAAGTTGTTATTGTCTAGAGAAAAATATGGTTTATTTGAGTATGATTCAATTAGTACATGTTTACGAGTGATTCAAATCACTTCTATCACATTTAAAATCATTTTGAAACAAATTTTTGATTACTCAAAATTAATTTTACACGTTCAAATGTATTTTTCATTGGAGTGTAATGAGAGTGCTATGTTCATGTCAAACTAATTGAGATGTTCGGATGTACTTCTTGATCCCTTTTAATTCTTGAGTATCTTGTTAAAAAAATGCATTTTTCATTAGATTATAATTGATTTGAAAGGAATAGAAGCACTTTTCGAGTGATTTAAGCCATTTTAGAATCACTCTTTTTTTTTTGGAACAAATTTTAGAACCACTCTCAAATATGCTATTAGTTAATACGTTATTTATCTTTGTAAGAGTCAATTATAAGATTTTGATTTTTGTTTTCCGATGACAATACAGCTGGTGGCGGAGGTGATAGCGACGTACTTGCTTGTGTTTGTGACATGTGGGGCGGGAGCATTGAACGCGAATGATGATCGGAGAGTGTCGCAGCTTGGTGCTTCGGTTGCAGGAGGCTTGATCGTGACGGTGATGATCTACGCCGTCGGACACATTTCCGGCGCCCACATGAACCCGGCTGTCACCATGGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTCTCACTCTTTAACTCTTTAGTAATAATTTCCTCTTGGTTGAAATTGTCAGTTATATTTTCTTCCTTAATCAATAACTTAATCATTTCCTCCCACATGTTATCTAATCTTGTCGGGCGATTATGTCCAAGTATTTTTATTTTTATTATTATTTTTCACTAGAGTTACTAGACATACAGTTTTGTGGTATTTACTTTTACCATTAATGTTTTTAGGATAGGAATATTTATTAAGTGATTAATGAAGACTCAATCACAAGGACACATAAGTCTTTATGCAAAACGTGGAGATCAATACAAACCAATAGAAAATTGATGGATCAAAATTATACTTTTAAAACTTAGGGATGAAAATTTAAAAAAAAAATTACAGAAATTTAACCAAAACAGGAATTGAACTTAAATTTTTATTTATGACGAATATAATATGTTTTATTTGATATTATTATTATATATTATTTCTCTAATTGCGTAAGCTAGGTAATTGATTAAAAATAAATAATAACTAGTGCACAAGTATATTAATATATTAATGATATTATATTAATTTTTTTTGTCAAGGAAAAAGCCTATAGTTGCCATTACAAAAAGAAAAAAAGAACTAATAGTTTATATAATATCATTAGCAAATAAATATACTATTAGTTAAACTAATGTTATAATTTAATAATGATCAAATCAACGTATTATAAAACTAATTTGATACATCATTTGTTTCAAATTTAACATTTAATATTTAGGGCGGTGCTAAGAATATAGGAACGAGGAGCACCTTGTTCTTTCCATAACTTTAATATATACATATAAATTTTTAAATCGGGGTTTTCAAGTAGTTGTTTACCTTCACTGTCAAAAAAAAAAAATTAAAATAATAAAATAAGGTAGTTGTTTACCTTCATCCCAATTTATAAAAATTTCTAATTACTTGTCGATTTTTGCCTCGTGATATATGGGATTCTTCACCCTCCAATTATTATAAAAAAAATAAAAGTAAAATAATAGGATGTATCTCAGGCAATCTTTGTGCAGCCTATATTCAAGTACTCAATCACAAGTTGCCACATGATAAATTTTTCTTTTATGTTATATATTTTTTTCCTTTTGTGATTAAAGGGAGGCATAGAGATGGGTATAGCTTCGGCTATGCCGGAGTGATGAGAGCGTTAAGAATGTATCAACTTAGTTGAGATATCCAAATGCACCTACTGATCTCTCGACCTTAGTACTTTTTCAAAAAAAAAAAAACATTGTTCAATGTTTTTCTCTAAATTGTATTATTGTAGAAAAAGAATTAAAACAAGAGATGAAAAATGGGAACACACAAGAGCCATTAATCGTGGTTTGGACTATTAGTTATGATAATTAGGAGAAGAATTTTGTGGAATTAAAATTGACCCTTTTTCTAATTTTTAGTTATAAAGTAGTCTAGAAGAATCACTATATGCATATAGGAAAATAAGTAAGAAATGAAATGATGACATAACAAAGAGGTAAAATGTAAGCCATATTTAGTGGCCACATGATGTAGGGTCAAGGGTTGTCCAAATATTATTCTACAGCCATAGCCACAAGGACAAGCATATGTGATCTTTTTATGGCCACAAGCTTGGCTTCATTTTGCCGGCCAAGGAATGGGATGGGACATTTAGGCAGCATAATTCCAACACTTAAGAAAAAATAGAGAGATAGAGATAGAGATGTCCACCAAAAAGTTCTTGGCCCATTGGGATCAGTGGAAGGTATAGGAGAAAGAGCAAAAGACAACACATATCTTCATGTGTGTTTTTATAGTCAAGATGGACATCTTTTACTTTATGGATATATTGATATTCACTTTTAGAATTCCAATCAAAACAATCAAAGTAATAATTTTAAATGTCTAGTCCTGATCTTTTTGGTTTTGGTTTAGGTTTTTTTAGGAGGGATTGTTCTGTTTTTTGTCTCAACATACTTTAATAATTCTTGGTTTGATTGAGTAAGGTTTTGTTAAACATGTAGATATCTAAGTTTATGTGTAAGTTATACTTGTTTGTGTTTAAAACAAGTTTAGTTTGCAAGTTTTTGCACCCATTTAATTTGACTTGCGACAAATATAATTTTCGTCGCATCTAAAACGTCATGAGTTTTGAGACTATTATACCATGTCATTGCAAATTTGTTGATGCAATCAATACTTCATCAGTACACTTGCAACACTTTGGAATTTGTAGCTAGCAAGTTTGAATTGAAATTAAAATTAATAAACATATATTTATTGATTTCTTTTTATTCTTTATTCTTTACTTGTTGATTTGTTTATGTCATAACAAAGACGTATTTAACCTAACGACCAAGGGATGGAACAATAGGAATCAAATGATTGTAATACGCAAGAATTTATTTTGGTGGGTGAATGAATGAGATGTCAAATTATATGGTTTGTGAATTGATTGAAAAGAAGGGAATTGGAAGATTGAAAGTAACATAGGAGAGTGATGTTGGTTTGGTTGGTAGGTTCCATTGTATGGAGCAGCTCAATTGAGTGGAGCAACTTGTGCAGCCTTTACACTGCGCATATTATTGCATCCAATTAAGCATTTGGGCACAACTACGCCATCCGGTTCAGACTTTCAAGCATTGGTTATGGAGATTGTTGTTACATTCTCAATGATGTTCGTCACAGCTGCTGTTGCAACTGACACCAAAGCAGTAAGATTCTTAACTAATACGTAAATATATCGAATAAATTATGAGTTTAGTTATTTATGAACTTTTCTGTTTCTATCAAATTAGTCTCTAAACTTGAAAAAGTGTCTAATATGTCTGTGAGCTTTAAAGGTATCGTCTAATAACTTTATCAACTTTCAATATTATGTATAATATAACTCTACATTTTTACTTCTGTATTTAGTACATCCATAAATCGATGACTCATAGACATAAAATTAAAAAATTGAAGAGCCTATTAGATGCAGAAATTGAAGTTTAAAACTTATTGCACAATTTATAAATTTTAAACGTTAAATATAAAATCAAAACTTGAGAAACTTATTAAACATTCTTTAAAGTTTAAGATTTAAACAGACATAGAGTTAAAAGTTTGATAGTTGAACTAGTAACCCAAACCTATATTTATTGTTTATTAATTTTTTAAAAATGTTTAATTTATTTAATTACTTTTTTTTTTTAAATCCCATGTTTTGAAAACTAAAGGTAAAAAAATGTTTTAAAAAATGTGAAATGCTTTCGTAAGTACTATTATTTATGAAAAAATGGAAAATATAAGACTCAATTCACATTTAAAATGGAATGAAATATTGATAGAAATAAGTGGAAGGTTTGAGTTCCACTCAATATTAAAAAAATAAAAATAAAATTTAGATATTTATTAAATATAATAACTATAAAGCAAACAAATTAAACTATAAGAAAATTTCCGGTATTTAAAAAATAGGAAAATTTTTACGGATAGAAAGAATTTCAAACTATTTACAAAAAATAACAAAAAAAATACTGATAGACATTGATAGACTTCTATCATTTCTATTAGTGATAAACTTTGATAGACTTCTATCAGCATCTATTACAACTATCTAAAAATTTGTCTATTTTGTATATATAGTTTTCCTTATTTTTCAATCTTTAAAAATCCTCCTTGTTGAAAATATTAAAGTGCAGTTATTCCATAAGTTTTGATTAATTTTGGAAAATGGTGGTTGTTTTGGCAGGTCGGAGAACTGGCAGGTATGGCAGTTGGCTCAGCAGTGTGTATCACTTCCATCTTAGCTGGGTAAGGAAAAATAACATTTTTTGGTTTGCCTTTTCAAAATTAAAATTATTTCCTTCAAAATAATAACCTTTTTTTTTTAAAAAAAAATTCTTATTTATTTAATTTTTCACATAGACTTATTTTTCAAAAATATCCTCCTTTACAAATCAATATGGACTTGTTAATACTTTATTACAAATTTTTAACTAATTTACAAAACCATACATAACAACAATTTTGATTTAAAAATGAAAATTAATTTTTGGAAACTTTTTGTAAACATCCCTTTAATATCTAAAAGTAGGCTTATCAACTACACAATTTTTGTGAAAACTCTCTTGAGTATCAAGATAATGCATAAACCAGATAAATCTAATGAAAATTTGTGTTAGCCACTATTCAGACCTGTATCAGGAGGGTCGATGAACCCTGTGAGGACTTTAGGACCAGCATTGGCAAGTGATTATTATAAAGGGCTTTGGGTATACTTTGTTGGGCCAGTTGTTGGAACACAATTGGGGGCATGGTCATATAAGTTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCACACTCTTTTTCACTCAAACTTCGAAGAATGTCAAGATCAGACGTTAGCGAAGGTGATCGTTAATATGAGCATTGCCTCAGCATCGTCTACCATCTTTAAAATATTTCAAATGTTTGAAAGATGTGAAATAGTTTGAGTGTTTAATGAGCAATGATCTCATGTACTATGCTATAAGCTTTTTTCTTATAGTTTAGTAGTGTATGAAGGCAAACTTCTAATAGTTTAGCAATGTATCAGAAGTAGCCTTTGTAATTTAACATCTTCTATATATATGAAAATTTTCTACATTTTTAA

mRNA sequence

CCCATGCCTCAGCTTCATTATAAAAAGGGTAGCTTGCAGCTTCAAGTTGATGTGCAAATAAGTTTTTTATAATAATATTAGCGTTTACGTTGAAACAGAGGGAGAGAATATTTTCAGTGTTCTTCCCCTTTGGCATTGGTGAATAATATTTGCCAAAGCTTTAGAGATCTAAACCACTTATAAAAGAAGTGCTTCCACTTCTTTGTATTTCGTTCCAACACAAGAAAGAAAAAAAATGAGTTCTGTTCAAAATCCTGAGCTCCCCAACCAAGAAGCTGTGGTCAATGTTAACGAATTCGTGTCTGTTGAAAACCCTGATTCCAAACGCTCCAAGTTTGGATCTTTCTTCAAAAACCCTTACCCTCCTGGGTTTTCTCGAAAGCTGGTGGCGGAGGTGATAGCGACGTACTTGCTTGTGTTTGTGACATGTGGGGCGGGAGCATTGAACGCGAATGATGATCGGAGAGTGTCGCAGCTTGGTGCTTCGGTTGCAGGAGGCTTGATCGTGACGGTGATGATCTACGCCGTCGGACACATTTCCGGCGCCCACATGAACCCGGCTGTCACCATGGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTCGGAGAACTGGCAGGTATGGCAGTTGGCTCAGCAGTGTGTATCACTTCCATCTTAGCTGGACCTGTATCAGGAGGGTCGATGAACCCTGTGAGGACTTTAGGACCAGCATTGGCAAGTGATTATTATAAAGGGCTTTGGGTATACTTTGTTGGGCCAGTTGTTGGAACACAATTGGGGGCATGGTCATATAAGTTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCACACTCTTTTTCACTCAAACTTCGAAGAATGTCAAGATCAGACGTTAGCGAAGGTGATCGTTAATATGAGCATTGCCTCAGCATCGTCTACCATCTTTAAAATATTTCAAATGTTTGAAAGATGTGAAATAGTTTGAGTGTTTAATGAGCAATGATCTCATGTACTATGCTATAAGCTTTTTTCTTATAGTTTAGTAGTGTATGAAGGCAAACTTCTAATAGTTTAGCAATGTATCAGAAGTAGCCTTTGTAATTTAACATCTTCTATATATATGAAAATTTTCTACATTTTTAA

Coding sequence (CDS)

ATGAGTTCTGTTCAAAATCCTGAGCTCCCCAACCAAGAAGCTGTGGTCAATGTTAACGAATTCGTGTCTGTTGAAAACCCTGATTCCAAACGCTCCAAGTTTGGATCTTTCTTCAAAAACCCTTACCCTCCTGGGTTTTCTCGAAAGCTGGTGGCGGAGGTGATAGCGACGTACTTGCTTGTGTTTGTGACATGTGGGGCGGGAGCATTGAACGCGAATGATGATCGGAGAGTGTCGCAGCTTGGTGCTTCGGTTGCAGGAGGCTTGATCGTGACGGTGATGATCTACGCCGTCGGACACATTTCCGGCGCCCACATGAACCCGGCTGTCACCATGGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTCGGAGAACTGGCAGGTATGGCAGTTGGCTCAGCAGTGTGTATCACTTCCATCTTAGCTGGACCTGTATCAGGAGGGTCGATGAACCCTGTGAGGACTTTAGGACCAGCATTGGCAAGTGATTATTATAAAGGGCTTTGGGTATACTTTGTTGGGCCAGTTGTTGGAACACAATTGGGGGCATGGTCATATAAGTTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCACACTCTTTTTCACTCAAACTTCGAAGAATGTCAAGATCAGACGTTAGCGAAGGTGATCGTTAA

Protein sequence

MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHFPWKQVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVSEGDR

Homology

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BLAST of Lsi03G014820 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 5.3e-55
Identity = 123/271 (45.39%), Postives = 153/271 (56.46%), Query Frame = 0


Query: 18  VNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAGALNANDDRR 77
           +++  + +N     + +     + +PP   +K+V+EV++T+LLVFVTCGA  +  +D  R
Sbjct: 17  IHDIGTAQNSSMPPTYYDRSLADIFPPHLLKKVVSEVVSTFLLVFVTCGAAGIYGSDKDR 76

Query: 78  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHFPWKQ------------- 137
           +SQLG SVAGGLIVTVMIYAVGHISGAHMNPAVT+AFA  RHFPW Q             
Sbjct: 77  ISQLGQSVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGSI 136

Query: 138 ----------------------------------------------------VGELAGMA 197
                                                               VGELAG+A
Sbjct: 137 CASFVLKAVLHPIAVLGTTTPTGPHWHSLVIEIIVTFNMMFVTLAVATDTRAVGELAGLA 196

Query: 198 VGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIR 223
           VGSAVCITSI AG VSGGSMNP RTLGPALAS+ Y GLW+YF+GPV+GT  GAW+Y +IR
Sbjct: 197 VGSAVCITSIFAGAVSGGSMNPARTLGPALASNLYTGLWIYFLGPVLGTLSGAWTYTYIR 256

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BLAST of Lsi03G014820 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.6e-54
Identity = 120/247 (48.58%), Postives = 145/247 (58.70%), Query Frame = 0


Query: 42  YPPGFSRKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHI 101
           +PP   +K+V+EV+AT+LLVF+TCGA  ++ +D  R+SQLG S+AGGLIVTVMIYAVGHI
Sbjct: 43  FPPHLLKKVVSEVVATFLLVFMTCGAAGISGSDLSRISQLGQSIAGGLIVTVMIYAVGHI 102

Query: 102 SGAHMNPAVTMAFAATRHFPWKQ------------------------------------- 161
           SGAHMNPAVT+AFA  RHFPW Q                                     
Sbjct: 103 SGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGAICASFVLKAVIHPVDVIGTTTPVGP 162

Query: 162 ----------------------------VGELAGMAVGSAVCITSILAGPVSGGSMNPVR 221
                                       VGELAG+AVGSAVCITSI AG +SGGSMNP R
Sbjct: 163 HWHSLVVEVIVTFNMMFVTLAVATDTRAVGELAGLAVGSAVCITSIFAGAISGGSMNPAR 222

Query: 222 TLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKLRRM-SR 223
           TLGPALAS+ + GLW+YF+GPV+GT  GAW+Y FIR  D P    S    S KLRR+ S+
Sbjct: 223 TLGPALASNKFDGLWIYFLGPVMGTLSGAWTYTFIRFEDTPKEGSSQKLSSFKLRRLRSQ 282

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BLAST of Lsi03G014820 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.6e-54
Identity = 116/237 (48.95%), Postives = 139/237 (58.65%), Query Frame = 0


Query: 42  YPPGFSRKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHI 101
           +PP   +K+++EV+AT+LLVFVTCGA ++   D+RR+SQLG SVAGGLIVTVMIYA GHI
Sbjct: 46  FPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNRRISQLGQSVAGGLIVTVMIYATGHI 105

Query: 102 SGAHMNPAVTMAFAATRHFPWKQ------------------------------------- 161
           SGAHMNPAVT++FA  RHFPW Q                                     
Sbjct: 106 SGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLHPIAVIGTTTPSGP 165

Query: 162 ----------------------------VGELAGMAVGSAVCITSILAGPVSGGSMNPVR 214
                                       VGELAG+AVGSAVCITSI AGPVSGGSMNP R
Sbjct: 166 HWHALLIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNPAR 225

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BLAST of Lsi03G014820 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-52
Identity = 121/263 (46.01%), Postives = 144/263 (54.75%), Query Frame = 0


Query: 27  PDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVA 86
           PDSK         + +PP   +K+++EV+AT+LLVFVTCGA ++   D+ R+SQLG SVA
Sbjct: 40  PDSKS------IADIFPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNARISQLGQSVA 99

Query: 87  GGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHFPWKQ---------------------- 146
           GGLIVTVMIYA GHISGAHMNPAVT++FA  RHFPW Q                      
Sbjct: 100 GGLIVTVMIYATGHISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAV 159

Query: 147 -------------------------------------------VGELAGMAVGSAVCITS 206
                                                      VGELAG+AVGSAVCITS
Sbjct: 160 LQPIAVIGTTTPSGPHWHALAIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITS 219

Query: 207 ILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHL- 223
           I AGPVSGGSMNP RTL PA+AS+ + GLW+YF+GPVVGT  GAW Y +IR  + P    
Sbjct: 220 IFAGPVSGGSMNPARTLAPAVASNVFTGLWIYFLGPVVGTLSGAWVYTYIRFEEAPAAAK 279

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BLAST of Lsi03G014820 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.2e-52
Identity = 123/280 (43.93%), Postives = 153/280 (54.64%), Query Frame = 0


Query: 8   ELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGA 67
           E+ +   V +V+   SV  P+         F + +PP   +K+++EV+AT+LLVFVTCGA
Sbjct: 19  EIHDLSTVQSVSAVPSVYYPEKS-------FADIFPPNLLKKVISEVVATFLLVFVTCGA 78

Query: 68  GALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHFPWKQ--- 127
            ++   D +R+SQLG SV GGLIVTVMIYA GHISGAHMNPAVT++FA  RHFPW Q   
Sbjct: 79  ASIYGEDMKRISQLGQSVVGGLIVTVMIYATGHISGAHMNPAVTLSFAFFRHFPWIQVPF 138

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 139 YWAAQFTGAMCAAFVLRAVLYPIEVLGTTTPTGPHWHALVIEIVVTFNMMFVTCAVATDS 198

Query: 188 --VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQ 220
             VGELAG+AVGSAVCITSI AGPVSGGSMNP RTL PA+AS+ Y GLW+YF+GPVVGT 
Sbjct: 199 RAVGELAGLAVGSAVCITSIFAGPVSGGSMNPARTLAPAVASNVYTGLWIYFLGPVVGTL 258

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BLAST of Lsi03G014820 vs. ExPASy TrEMBL
Match: G8XUV3 (Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.4e-105
Identity = 210/287 (73.17%), Postives = 218/287 (75.96%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS+QNP+L NQEAVV+VNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA ALNA+D RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQ-------------------------------------------------------- 180
           PWKQ                                                        
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 ---------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 223
                    VGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

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BLAST of Lsi03G014820 vs. ExPASy TrEMBL
Match: A0A1L7B7J0 (LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.4e-105
Identity = 210/287 (73.17%), Postives = 218/287 (75.96%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS+QNP+L NQEAVV+VNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA ALNA+D RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQ-------------------------------------------------------- 180
           PWKQ                                                        
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 ---------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 223
                    VGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. ExPASy TrEMBL
Match: A0A5D3DQ37 (Aquaporin NIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G008030 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 209/287 (72.82%), Postives = 215/287 (74.91%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS+ NPEL NQE VV+VNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSI-NPELSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA ALNA+D +RVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDPQRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQ-------------------------------------------------------- 180
           PWKQ                                                        
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 ---------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 223
                    VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASD YKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDNYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. ExPASy TrEMBL
Match: A0A1S3B807 (aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487002 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 209/287 (72.82%), Postives = 215/287 (74.91%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS+ NPEL NQE VV+VNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSI-NPELSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA ALNA+D +RVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDPQRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQ-------------------------------------------------------- 180
           PWKQ                                                        
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 ---------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 223
                    VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASD YKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDNYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. ExPASy TrEMBL
Match: A0A6J1F1I0 (aquaporin NIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111441338 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.5e-97
Identity = 199/288 (69.10%), Postives = 208/288 (72.22%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MS VQNPELPN E VV+V + VSVENPDSK S F S F+N YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSVENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA AL+A+D+RRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVT+AFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQV------------------------------------------------------- 180
           PWKQV                                                       
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 ----------GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 224
                     GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALAS+YYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. NCBI nr
Match: XP_038905688.1 (aquaporin NIP2-1-like [Benincasa hispida])

HSP 1 Score: 400.2 bits (1027), Expect = 1.2e-107
Identity = 215/288 (74.65%), Postives = 219/288 (76.04%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSSVQN ELPNQE VVNVNEFVSVENPDS+RSKFGSFFKNPYPPGFSRKLVAEVI+TYLL
Sbjct: 55  MSSVQNSELPNQETVVNVNEFVSVENPDSRRSKFGSFFKNPYPPGFSRKLVAEVISTYLL 114

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA ALNAND RRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF
Sbjct: 115 VFVTCGAAALNANDQRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 174

Query: 121 PWKQ-------------------------------------------------------- 180
           PWKQ                                                        
Sbjct: 175 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDFQALVMEIVVTFSMMFVT 234

Query: 181 ---------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 224
                    VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 235 AAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 294

[Close]

BLAST of Lsi03G014820 vs. NCBI nr
Match: NP_001267699.1 (aquaporin NIP2-1-like [Cucumis sativus] >ACU29603.1 Si transport-like protein 1 [Cucumis sativus] >AOW42547.1 silicon transporter Lsi1 [Cucumis sativus] >APT69293.1 LSi1 [Cucumis sativus var. sativus] >KGN59570.1 hypothetical protein Csa_001739 [Cucumis sativus])

HSP 1 Score: 391.0 bits (1003), Expect = 7.1e-105
Identity = 210/287 (73.17%), Postives = 218/287 (75.96%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS+QNP+L NQEAVV+VNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA ALNA+D RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQ-------------------------------------------------------- 180
           PWKQ                                                        
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 ---------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 223
                    VGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. NCBI nr
Match: XP_008443411.1 (PREDICTED: aquaporin NIP2-1-like [Cucumis melo] >KAA0053759.1 aquaporin NIP2-1-like [Cucumis melo var. makuwa] >TYK25642.1 aquaporin NIP2-1-like [Cucumis melo var. makuwa])

HSP 1 Score: 382.1 bits (980), Expect = 3.3e-102
Identity = 209/287 (72.82%), Postives = 215/287 (74.91%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS+ NPEL NQE VV+VNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSI-NPELSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA ALNA+D +RVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDPQRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQ-------------------------------------------------------- 180
           PWKQ                                                        
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 ---------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 223
                    VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASD YKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDNYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. NCBI nr
Match: XP_022934054.1 (aquaporin NIP2-1-like [Cucurbita moschata])

HSP 1 Score: 365.5 bits (937), Expect = 3.2e-97
Identity = 199/288 (69.10%), Postives = 208/288 (72.22%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MS VQNPELPN E VV+V + VSVENPDSK S F S F+N YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSVENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA AL+A+D+RRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVT+AFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQV------------------------------------------------------- 180
           PWKQV                                                       
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 ----------GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 224
                     GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALAS+YYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. NCBI nr
Match: XP_023526670.1 (aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 365.2 bits (936), Expect = 4.1e-97
Identity = 198/288 (68.75%), Postives = 208/288 (72.22%), Query Frame = 0


Query: 1   MSSVQNPELPNQEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MS VQNPELPN E VV+V + VS+ENPDSK S F S F+N YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 120
           VFVTCGA AL+A+D+RRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVT+AFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQV------------------------------------------------------- 180
           PWKQV                                                       
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 ----------GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 224
                     GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALAS+YYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

[Close]

BLAST of Lsi03G014820 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 149.8 bits (377), Expect = 2.5e-36
Identity = 90/244 (36.89%), Postives = 117/244 (47.95%), Query Frame = 0


Query: 46  FSRKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAH 105
           F +KL+AEV+ TY L+F  C A A+N   D+ V+  G ++  GL V V++Y++GHISGAH
Sbjct: 50  FLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISGAH 109

Query: 106 MNPAVTMAFAATRHFPWKQV---------------------------------------- 165
            NPAVT+AFA+   FP KQV                                        
Sbjct: 110 FNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGTL 169

Query: 166 ---------------------------------GELAGMAVGSAVCITSILAGPVSGGSM 217
                                            GELAG+AVGS V +  I+AGPVSG SM
Sbjct: 170 PSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGASM 229

[Close]

BLAST of Lsi03G014820 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 141.0 bits (354), Expect = 1.2e-33
Identity = 86/242 (35.54%), Postives = 113/242 (46.69%), Query Frame = 0


Query: 47  SRKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHM 106
           ++KL+AE+I TY ++F  CG   +N      ++  G  V  GLIV VMIY+ GHISGAH 
Sbjct: 42  TQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHF 101

Query: 107 NPAVTMAFAATRHFPWKQV----------------------------------------- 166
           NPAVT+ FA  R FPW QV                                         
Sbjct: 102 NPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQA 161

Query: 167 -------------------------GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGP 222
                                    GELAG+AVG  + +   +AGP+SG SMNP R+LGP
Sbjct: 162 LVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLGP 221

[Close]

BLAST of Lsi03G014820 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 138.3 bits (347), Expect = 7.7e-33
Identity = 83/229 (36.24%), Postives = 108/229 (47.16%), Query Frame = 0


Query: 47  SRKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHM 106
           ++KL+AE+I TY +VF  CG   +N      ++  G  V  GLIV VMIY+ GHISGAH 
Sbjct: 42  TQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHF 101

Query: 107 NPAVTMAFAATRHFPWKQ------------------------------------------ 166
           NPAVT+ FA  R FPW Q                                          
Sbjct: 102 NPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPARA 161

Query: 167 ------------------------VGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGP 210
                                   VGELAG+AVG  + +   +AGP+SG SMNP R+LGP
Sbjct: 162 LVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGP 221

[Close]

BLAST of Lsi03G014820 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 137.1 bits (344), Expect = 1.7e-32
Identity = 90/275 (32.73%), Postives = 125/275 (45.45%), Query Frame = 0


Query: 12  QEAVVNVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAGALN 71
           ++ V +  E   + NP   + K  S      P  F +KL+AE + TY LVF  C +  +N
Sbjct: 22  KDEVEHQQEMEDIHNPRPLK-KQDSLLSVSVP--FLQKLIAEFLGTYFLVFTGCASVVVN 81

Query: 72  ANDDRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHFPWKQV------ 131
             +D  V+  G ++  GL + V+IY++GHISGAH+NPAVT+AFA+   FP KQV      
Sbjct: 82  MQNDNVVTLPGIAIVWGLTIMVLIYSLGHISGAHINPAVTIAFASCGRFPLKQVPAYVIS 141

Query: 132 ------------------------------------------------------------ 191
                                                                       
Sbjct: 142 QVIGSTLAAATLRLLFGLDHDVCSGKHDVFIGSSPVGSDLQAFTMEFIVTFYLMFIISGV 201

Query: 192 -------GELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPV 214
                  GELAG+A+GS V +  ++A PVS  SMNP R+LGPAL    YKG+W+Y V P 
Sbjct: 202 ATDNRAIGELAGLAIGSTVLLNVLIAAPVSSASMNPGRSLGPALVYGCYKGIWIYLVAPT 261

[Close]

BLAST of Lsi03G014820 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 129.0 bits (323), Expect = 4.7e-30
Identity = 73/221 (33.03%), Postives = 106/221 (47.96%), Query Frame = 0


Query: 43  PPGFS--RKLVAEVIATYLLVFVTCGAGALNANDDRRVSQLGASVAGGLIVTVMIYAVGH 102
           PP  S  RKL AE + T +L+F       +N   D   + +G + + GL V ++I + GH
Sbjct: 73  PPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAVMIVILSTGH 132

Query: 103 ISGAHMNPAVTMAFAATRHFPWKQ------------------------------------ 162
           ISGAH+NPAVT+AFAA +HFPWK                                     
Sbjct: 133 ISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPTMSGGVTVPTV 192

Query: 163 -----------------------------VGELAGMAVGSAVCITSILAGPVSGGSMNPV 197
                                        VGELAG+AVG+ V +  ++AGP +  SMNPV
Sbjct: 193 GLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPATSASMNPV 252

The following BLAST results are available for this feature:

BLAST of Lsi03G014820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q19KC1	5.3e-55	45.39	Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2	[more]
Q6Z2T3	2.6e-54	48.58	Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1	[more]
Q9ATN2	2.6e-54	48.95	Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1	[more]
Q9AT74	1.4e-52	46.01	Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1	[more]
Q67WJ8	3.2e-52	43.93	Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1	[more]

BLAST of Lsi03G014820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
G8XUV3	3.4e-105	73.17	Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1	[more]
A0A1L7B7J0	3.4e-105	73.17	LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1	[more]
A0A5D3DQ37	1.6e-102	72.82	Aquaporin NIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35...	[more]
A0A1S3B807	1.6e-102	72.82	aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487002 PE=3 SV=1	[more]
A0A6J1F1I0	1.5e-97	69.10	aquaporin NIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111441338 PE=3 SV=1	[more]

BLAST of Lsi03G014820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905688.1	1.2e-107	74.65	aquaporin NIP2-1-like [Benincasa hispida]	[more]
NP_001267699.1	7.1e-105	73.17	aquaporin NIP2-1-like [Cucumis sativus] >ACU29603.1 Si transport-like protein 1 ...	[more]
XP_008443411.1	3.3e-102	72.82	PREDICTED: aquaporin NIP2-1-like [Cucumis melo] >KAA0053759.1 aquaporin NIP2-1-l...	[more]
XP_022934054.1	3.2e-97	69.10	aquaporin NIP2-1-like [Cucurbita moschata]	[more]
XP_023526670.1	4.1e-97	68.75	aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G014820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18910.1	2.5e-36	36.89	NOD26-like intrinsic protein 1;2	[more]
AT5G37820.1	1.2e-33	35.54	NOD26-like intrinsic protein 4;2	[more]
AT5G37810.1	7.7e-33	36.24	NOD26-like intrinsic protein 4;1	[more]
AT4G19030.1	1.7e-32	32.73	NOD26-like major intrinsic protein 1	[more]
AT1G80760.1	4.7e-30	33.03	NOD26-like intrinsic protein 6;1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 87..111 score: 50.67 coord: 48..67 score: 38.08
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 46..125 e-value: 4.5E-18 score: 65.8 coord: 125..188 e-value: 1.1E-16 score: 61.2
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 11..124 e-value: 4.1E-25 score: 90.0
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 125..201 e-value: 1.2E-18 score: 69.1
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 43..201
None	No IPR available	PANTHER	PTHR45724:SF30	MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATED	coord: 123..207 coord: 22..125
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 22..125
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 123..207
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 105..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014820.1	Lsi03G014820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0015267	channel activity