Lsi03G014800 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G014800
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	aquaporin NIP2-1-like
Location	chr03: 25765051 .. 25766008 (-)
RNA-Seq Expression	Lsi03G014800
Synteny	Lsi03G014800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCCACTGTATGCAGCAGCTCAATTAAGTGGAGCTACAACAGCAGCCTTTACACTGCGCATATTATTAGACCCAATTCAAGACTTAGGTACAACTTCACCTTCTGGACCAGCGTTTAAAGCACTTATCATGGAGATTGTTGTTTCATTTTGTATGATGTTCATCACTTCTGCGGTTGCTACCGACACGAAAGCGGTAAGAACATCTTATTATTGTTAACCTCTTACTCCCAACCTTCTCTTACTTTCATTTGGCTAAGTTTCTCCATGTGATTTTTGGCAGATAGGAGAGCTTGGAGGCATAGCAGTGGGGTCAGCTGTATGTATCACATCCATCTTTGCTGGGTAAGTAAAACAAAAATAGTTGTTTTAGTTTGGCTTTTTATTCTGTAAAAGCTTTGTGTGTATTTGGAGAAAATCTCAAGGAAGATCTCAATTTGGTTAAATTGCAAATTTGTCCCTATAGTTTGAAGAAAGTTAGAACTTAGTCCCTATGGTTTGCAATTAAACCTTAAAACATATTCTATGTCCAACAAAATGTGCATATAGTAGATGAAAATGTATCTTTTTAGTCTCGAGTTTTGAAGAATAGGTCTATTTGGTTTATGAATTTTCAAAATGTAACTTTCTAGTTATTTTTTGAATTTTTAAGAATAGGTGTGTTTCAGTGTTTGGTCTTTAAACGTAACTTTTGAGTCTGAATACGTACAATTTTTTCAAAATTATAAATTAAGGTTAAGAGATAGAATCTGATATAAGTTGGGTGAACCATGTGTAGGCCAATATCGGGCGGGTCGATGAACCCAGCAAGGTCAATAGGACCAGCGATTGCAAGTTCACATTACGAAGGGATTTGGGTGTACCTGGTCGGCCCAGTTGCAGGAACATTGCTGGGGGCATTTTCATATAACTTCGTACGAGCCACTGAAAAACATTCCCATCCACTTTCTTTACAATGA

mRNA sequence

GTTCCACTGTATGCAGCAGCTCAATTAAGTGGAGCTACAACAGCAGCCTTTACACTGCGCATATTATTAGACCCAATTCAAGACTTAGGTACAACTTCACCTTCTGGACCAGCGTTTAAAGCACTTATCATGGAGATTGTTGTTTCATTTTGTATGATGTTCATCACTTCTGCGGTTGCTACCGACACGAAAGCGATAGGAGAGCTTGGAGGCATAGCAGTGGGGTCAGCTGTATGTATCACATCCATCTTTGCTGGGCCAATATCGGGCGGGTCGATGAACCCAGCAAGGTCAATAGGACCAGCGATTGCAAGTTCACATTACGAAGGGATTTGGGTGTACCTGGTCGGCCCAGTTGCAGGAACATTGCTGGGGGCATTTTCATATAACTTCGTACGAGCCACTGAAAAACATTCCCATCCACTTTCTTTACAATGA

Coding sequence (CDS)

GTTCCACTGTATGCAGCAGCTCAATTAAGTGGAGCTACAACAGCAGCCTTTACACTGCGCATATTATTAGACCCAATTCAAGACTTAGGTACAACTTCACCTTCTGGACCAGCGTTTAAAGCACTTATCATGGAGATTGTTGTTTCATTTTGTATGATGTTCATCACTTCTGCGGTTGCTACCGACACGAAAGCGATAGGAGAGCTTGGAGGCATAGCAGTGGGGTCAGCTGTATGTATCACATCCATCTTTGCTGGGCCAATATCGGGCGGGTCGATGAACCCAGCAAGGTCAATAGGACCAGCGATTGCAAGTTCACATTACGAAGGGATTTGGGTGTACCTGGTCGGCCCAGTTGCAGGAACATTGCTGGGGGCATTTTCATATAACTTCGTACGAGCCACTGAAAAACATTCCCATCCACTTTCTTTACAATGA

Protein sequence

VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVATDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVAGTLLGAFSYNFVRATEKHSHPLSLQ

Homology

[Close]

BLAST of Lsi03G014800 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 8.0e-44
Identity = 87/136 (63.97%), Postives = 112/136 (82.35%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VP Y AAQ +GA  AAF LR +L PI+ LGTT+P+GP + AL++EIVV+F MMF+T AVA
Sbjct: 129 VPFYWAAQFTGAMCAAFVLRAVLYPIEVLGTTTPTGPHWHALVIEIVVTFNMMFVTCAVA 188

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TD++A+GEL G+AVGSAVCITSIFAGP+SGGSMNPAR++ PA+AS+ Y G+W+Y +GPV 
Sbjct: 189 TDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNPARTLAPAVASNVYTGLWIYFLGPVV 248

Query: 121 GTLLGAFSYNFVRATE 137
           GTL GA+ Y ++R  E
Sbjct: 249 GTLSGAWVYTYIRFEE 264

[Close]

BLAST of Lsi03G014800 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 176.8 bits (447), Expect = 1.8e-43
Identity = 85/140 (60.71%), Postives = 114/140 (81.43%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VP Y AAQ +G+  A+F L+ +L PI  LGTT+P+GP + +L++EI+V+F MMF+T AVA
Sbjct: 124 VPFYWAAQFTGSICASFVLKAVLHPIAVLGTTTPTGPHWHSLVIEIIVTFNMMFVTLAVA 183

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDT+A+GEL G+AVGSAVCITSIFAG +SGGSMNPAR++GPA+AS+ Y G+W+Y +GPV 
Sbjct: 184 TDTRAVGELAGLAVGSAVCITSIFAGAVSGGSMNPARTLGPALASNLYTGLWIYFLGPVL 243

Query: 121 GTLLGAFSYNFVRATEKHSH 141
           GTL GA++Y ++R  E  SH
Sbjct: 244 GTLSGAWTYTYIRFEEAPSH 263

[Close]

BLAST of Lsi03G014800 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 8.8e-43
Identity = 85/136 (62.50%), Postives = 111/136 (81.62%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VP Y AAQ +GA  AAF L+ +L PI  +GTT+PSGP + AL++EIVV+F MMF+T AVA
Sbjct: 129 VPFYWAAQFTGAMCAAFVLKAVLHPIAVIGTTTPSGPHWHALLIEIVVTFNMMFVTCAVA 188

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TD++A+GEL G+AVGSAVCITSIFAGP+SGGSMNPAR++ PA+AS+ + G+W+Y +GPV 
Sbjct: 189 TDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNPARTLAPAVASNVFTGLWIYFLGPVI 248

Query: 121 GTLLGAFSYNFVRATE 137
           GTL GA+ Y ++R  E
Sbjct: 249 GTLSGAWVYTYIRFEE 264

[Close]

BLAST of Lsi03G014800 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-42
Identity = 85/136 (62.50%), Postives = 110/136 (80.88%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VP Y AAQ +GA  AAF L+ +L PI  +GTT+PSGP + AL +EIVV+F MMF+T AVA
Sbjct: 132 VPFYWAAQFTGAMCAAFVLKAVLQPIAVIGTTTPSGPHWHALAIEIVVTFNMMFVTCAVA 191

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TD++A+GEL G+AVGSAVCITSIFAGP+SGGSMNPAR++ PA+AS+ + G+W+Y +GPV 
Sbjct: 192 TDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNPARTLAPAVASNVFTGLWIYFLGPVV 251

Query: 121 GTLLGAFSYNFVRATE 137
           GTL GA+ Y ++R  E
Sbjct: 252 GTLSGAWVYTYIRFEE 267

[Close]

BLAST of Lsi03G014800 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 4.4e-42
Identity = 80/133 (60.15%), Postives = 111/133 (83.46%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VP Y AAQ +GA  A+F L+ ++ P+  +GTT+P GP + +L++E++V+F MMF+T AVA
Sbjct: 126 VPFYWAAQFTGAICASFVLKAVIHPVDVIGTTTPVGPHWHSLVVEVIVTFNMMFVTLAVA 185

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDT+A+GEL G+AVGSAVCITSIFAG ISGGSMNPAR++GPA+AS+ ++G+W+Y +GPV 
Sbjct: 186 TDTRAVGELAGLAVGSAVCITSIFAGAISGGSMNPARTLGPALASNKFDGLWIYFLGPVM 245

Query: 121 GTLLGAFSYNFVR 134
           GTL GA++Y F+R
Sbjct: 246 GTLSGAWTYTFIR 258

[Close]

BLAST of Lsi03G014800 vs. ExPASy TrEMBL
Match: A0A172B0F5 (Silicon transporter 3 OS=Cucurbita ficifolia OX=37645 GN=Lsi3 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.7e-66
Identity = 132/145 (91.03%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R TEKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTTEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. ExPASy TrEMBL
Match: A0A6J1J0H5 (aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482345 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.2e-65
Identity = 131/145 (90.34%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R +EKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTSEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. ExPASy TrEMBL
Match: A0A172B7P2 (Silicon transporter 3 OS=Cucurbita moschata OX=3662 GN=Lsi3 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.2e-65
Identity = 131/145 (90.34%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R +EKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTSEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. ExPASy TrEMBL
Match: A0A6J1FB40 (aquaporin NIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111442441 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.2e-65
Identity = 131/145 (90.34%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R +EKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTSEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. ExPASy TrEMBL
Match: A0A1S3B8P6 (aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.5e-64
Identity = 128/143 (89.51%), Postives = 137/143 (95.80%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPIQDLGTTSP GPA KAL+MEIVVSFCMMF+TSAVA
Sbjct: 117 VPLYAAAQLSGATSAAFTLRILLDPIQDLGTTSPHGPALKALVMEIVVSFCMMFVTSAVA 176

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGELGGIAVGSAVCI+SIFAGPISGGSMNPARSIGPAIASS YEGIWVY++GP+ 
Sbjct: 177 TDTKAIGELGGIAVGSAVCISSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYMIGPIT 236

Query: 121 GTLLGAFSYNFVRATEKHSHPLS 144
           GTLL AFSYNF+RATEKH+H LS
Sbjct: 237 GTLLAAFSYNFIRATEKHTHSLS 259

[Close]

BLAST of Lsi03G014800 vs. NCBI nr
Match: XP_038906096.1 (aquaporin NIP2-2-like [Benincasa hispida])

HSP 1 Score: 264.6 bits (675), Expect = 5.0e-67
Identity = 136/145 (93.79%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGP FKAL+MEIVVSFCMMF+TSAVA
Sbjct: 123 VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPIFKALLMEIVVSFCMMFVTSAVA 182

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKA+GEL GIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASS YEGIWVYLVGPVA
Sbjct: 183 TDTKAVGELAGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSRYEGIWVYLVGPVA 242

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGAFSYNF+RATEKHS  LSLQ
Sbjct: 243 GTLLGAFSYNFIRATEKHSRSLSLQ 267

[Close]

BLAST of Lsi03G014800 vs. NCBI nr
Match: AKB95556.1 (silicon transporter 3 [Cucurbita ficifolia])

HSP 1 Score: 259.6 bits (662), Expect = 1.6e-65
Identity = 132/145 (91.03%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R TEKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTTEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. NCBI nr
Match: AKH49406.1 (silicon transporter 3 [Cucurbita moschata])

HSP 1 Score: 258.1 bits (658), Expect = 4.6e-65
Identity = 131/145 (90.34%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R +EKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTSEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. NCBI nr
Match: XP_022935648.1 (aquaporin NIP2-1-like [Cucurbita moschata])

HSP 1 Score: 258.1 bits (658), Expect = 4.6e-65
Identity = 131/145 (90.34%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R +EKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTSEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. NCBI nr
Match: XP_022983857.1 (aquaporin NIP2-1-like [Cucurbita maxima])

HSP 1 Score: 258.1 bits (658), Expect = 4.6e-65
Identity = 131/145 (90.34%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VPLYAAAQLSGAT+AAFTLRILLDPI++LGTTSPSGP FKALI+EIVVSFCMMF+TSAVA
Sbjct: 120 VPLYAAAQLSGATSAAFTLRILLDPIKELGTTSPSGPEFKALIVEIVVSFCMMFVTSAVA 179

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDTKAIGEL GIAVGS+VCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA
Sbjct: 180 TDTKAIGELAGIAVGSSVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 239

Query: 121 GTLLGAFSYNFVRATEKHSHPLSLQ 146
           GTLLGA+SYNF+R +EKH H LSLQ
Sbjct: 240 GTLLGAWSYNFIRTSEKHGHQLSLQ 264

[Close]

BLAST of Lsi03G014800 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 141.7 bits (356), Expect = 4.5e-34
Identity = 74/139 (53.24%), Postives = 97/139 (69.78%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLD--PIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSA 60
           VPLY  AQL+G+  A+ TLR++ +  P    GTT P+  + +AL+ EI++SF +MF+ S 
Sbjct: 120 VPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTT-PTDSSGQALVAEIIISFLLMFVISG 179

Query: 61  VATDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGP 120
           VATD++A GEL GIAVG  + +    AGPISG SMNPARS+GPAI    Y+GIWVY+VGP
Sbjct: 180 VATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGP 239

Query: 121 VAGTLLGAFSYNFVRATEK 138
             G   G F YNF+R T+K
Sbjct: 240 FVGIFAGGFVYNFMRFTDK 257

[Close]

BLAST of Lsi03G014800 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 139.8 bits (351), Expect = 1.7e-33
Identity = 72/139 (51.80%), Postives = 95/139 (68.35%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRIL--LDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSA 60
           VPLY  AQ +G+  A+ TLR++  + P    GTT    PA +AL+ EI++SF +MF+ S 
Sbjct: 120 VPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPA-RALVAEIIISFLLMFVISG 179

Query: 61  VATDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGP 120
           VATD +A+GEL GIAVG  + +    AGPISG SMNPARS+GPA+    Y+ IWVY+VGP
Sbjct: 180 VATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGPALVMGVYKHIWVYIVGP 239

Query: 121 VAGTLLGAFSYNFVRATEK 138
           V G + G F YN +R T+K
Sbjct: 240 VLGVISGGFVYNLIRFTDK 257

[Close]

BLAST of Lsi03G014800 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 135.2 bits (339), Expect = 4.2e-32
Identity = 69/145 (47.59%), Postives = 97/145 (66.90%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGT--------TSPSGPAFKALIMEIVVSFCM 60
           VP Y  +Q+ G+T AA TLR+L    QD+ +        T PSG   ++ ++E +++F +
Sbjct: 129 VPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGTLPSGSNLQSFVIEFIITFYL 188

Query: 61  MFITSAVATDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIW 120
           MF+ S VATD +AIGEL G+AVGS V +  I AGP+SG SMNP RS+GPA+  S Y G+W
Sbjct: 189 MFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGASMNPGRSLGPAMVYSCYRGLW 248

Query: 121 VYLVGPVAGTLLGAFSYNFVRATEK 138
           +Y+V P+ G + GA+ YN VR T+K
Sbjct: 249 IYIVSPIVGAVSGAWVYNMVRYTDK 273

[Close]

BLAST of Lsi03G014800 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 132.5 bits (332), Expect = 2.7e-31
Identity = 63/141 (44.68%), Postives = 94/141 (66.67%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VP+Y  AQ+  + +AAF L+ + +P    G T P+    +A  +E ++SF +MF+ +AVA
Sbjct: 157 VPVYIGAQVMASVSAAFALKAVFEPTMSGGVTVPTVGLSQAFALEFIISFNLMFVVTAVA 216

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDT+A+GEL GIAVG+ V +  + AGP +  SMNP R++GPAIA+++Y  IWVYL  P+ 
Sbjct: 217 TDTRAVGELAGIAVGATVMLNILIAGPATSASMNPVRTLGPAIAANNYRAIWVYLTAPIL 276

Query: 121 GTLLGAFSYNFVRATEKHSHP 142
           G L+GA +Y  V+  E+   P
Sbjct: 277 GALIGAGTYTIVKLPEEDEAP 297

[Close]

BLAST of Lsi03G014800 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 129.0 bits (323), Expect = 3.0e-30
Identity = 63/141 (44.68%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 1   VPLYAAAQLSGATTAAFTLRILLDPIQDLGTTSPSGPAFKALIMEIVVSFCMMFITSAVA 60
           VP Y AAQ+S +  A+F L+ +  P    G T PS    +A  +E +++F ++F+ +AVA
Sbjct: 155 VPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLGQAFALEFIITFILLFVVTAVA 214

Query: 61  TDTKAIGELGGIAVGSAVCITSIFAGPISGGSMNPARSIGPAIASSHYEGIWVYLVGPVA 120
           TDT+A+GEL GIAVG+ V +  + AGP +GGSMNP R++GPA+AS +Y  +WVYLV P  
Sbjct: 215 TDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPAVASGNYRSLWVYLVAPTL 274

Query: 121 GTLLGAFSYNFVRATEKHSHP 142
           G + GA  Y  V+  +  + P
Sbjct: 275 GAISGAAVYTGVKLNDSVTDP 295

The following BLAST results are available for this feature:

BLAST of Lsi03G014800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67WJ8	8.0e-44	63.97	Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1	[more]
Q19KC1	1.8e-43	60.71	Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2	[more]
Q9ATN2	8.8e-43	62.50	Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1	[more]
Q9AT74	1.2e-42	62.50	Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1	[more]
Q6Z2T3	4.4e-42	60.15	Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1	[more]

BLAST of Lsi03G014800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A172B0F5	7.7e-66	91.03	Silicon transporter 3 OS=Cucurbita ficifolia OX=37645 GN=Lsi3 PE=2 SV=1	[more]
A0A6J1J0H5	2.2e-65	90.34	aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482345 PE=3 SV=1	[more]
A0A172B7P2	2.2e-65	90.34	Silicon transporter 3 OS=Cucurbita moschata OX=3662 GN=Lsi3 PE=2 SV=1	[more]
A0A6J1FB40	2.2e-65	90.34	aquaporin NIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111442441 PE=3 SV=1	[more]
A0A1S3B8P6	1.5e-64	89.51	aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487003 PE=3 SV=1	[more]

BLAST of Lsi03G014800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906096.1	5.0e-67	93.79	aquaporin NIP2-2-like [Benincasa hispida]	[more]
AKB95556.1	1.6e-65	91.03	silicon transporter 3 [Cucurbita ficifolia]	[more]
AKH49406.1	4.6e-65	90.34	silicon transporter 3 [Cucurbita moschata]	[more]
XP_022935648.1	4.6e-65	90.34	aquaporin NIP2-1-like [Cucurbita moschata]	[more]
XP_022983857.1	4.6e-65	90.34	aquaporin NIP2-1-like [Cucurbita maxima]	[more]

BLAST of Lsi03G014800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37820.1	4.5e-34	53.24	NOD26-like intrinsic protein 4;2	[more]
AT5G37810.1	1.7e-33	51.80	NOD26-like intrinsic protein 4;1	[more]
AT4G18910.1	4.2e-32	47.59	NOD26-like intrinsic protein 1;2	[more]
AT1G80760.1	2.7e-31	44.68	NOD26-like intrinsic protein 6;1	[more]
AT4G10380.1	3.0e-30	44.68	NOD26-like intrinsic protein 5;1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 44..62 score: 21.31 coord: 75..97 score: 40.01 coord: 112..132 score: 37.44
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 1..129 e-value: 8.7E-29 score: 100.8
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 1..141 e-value: 3.3E-34 score: 119.8
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 1..140
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 1..143
None	No IPR available	PANTHER	PTHR45724:SF30	MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATED	coord: 1..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014800.1	Lsi03G014800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015267	channel activity