Lsi03G014600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G014600
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectate lyase
Location	chr03: 25486590 .. 25488810 (+)
RNA-Seq Expression	Lsi03G014600
Synteny	Lsi03G014600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGACAGCTTAGAGAAATAAAGAAAGAAAAAAAACTCAGGGTTGCTTCACATTAATTTTGGTCTGGCAAGGTCTTGAATAAACTTAGGTCTTTTACCTATTTGGGGTTCTTTCTTAGACCTTGACCAGCCCAAAAAGCTTGCTTTCAGAAAGAACCTCCAAAAATTCAAAAATAAATAAATAAATAAAAGGTCCATTTTTTTTTGCTTGGGTCTTCAAGAAGAAAAAGAAAAATGATGGCAGGCTGTTCAATTAAGTTTGTGTTGGTGTTTGTTTCAATGGCTGTTCTTATGCCAACTCTAAGAGCTGCCATTGCTGAATATGATGACTTTTTGCTCCAAAAGGCTGAAGAAGCCAAGCAAGCGTCTCTTCAAGCCTTCCATCCTGACCCCATGAATGTCACTGACCATTTCAACCAACAAGTTCATTTGTATGTTTAATCTTTCTTTGTTTTTCTGTTTTTTTTTTTTTTTTTTTAACATTGACATTGACGTTGACATTGATATTGATATTGACATCCATATTTTTTTTTTTTTTTTGGTTGTATGTAGAGCTTTGGAGGGAATAGAGGGAAACAATAGCACTAGGAGGAGTTTGAGGAAATACAACGGTCCGTGCTTGGCGACGAATCCGATTGATCGTTGCTGGCGTTGCGACCCAAATTGGGCCAAGAACCGTAAGAAGCTTGCCACTTGTGTGCTCGGTTTCGGCCGTAAGACCACCGGTGGCAAAAGAGGACGTATCTACGTTGTGACCGATCCATCGGATAACGATGTTATTAACCCTAAACCTGGAACCCTCCGATATGCTGCTATTCAAAAACGACCATTATGGATCATTTTTGGGCGCAGTATGATCATCAGGCTATCCAAGGAGTTGTTGATAGTCAGCCACAAGACCATTGATGCTCGCGGTGCCAATGTTCACATTGCCTATGGTGCTGGTCTTAGTATTCAATTCTCAAGAAATGTGATCATCCATGGCCTTCACATCCACCACGTCATTTCAACCGGCGGTGGCATGATCCGAGATGGTGCTGACCATGTCGGACTTCGAACTGTTAGTGACGGAGACGGTATCTCCATCTTTGGATCATCCAATATCTGGCTCGATCATCTCTCCATGTCTAACTGTCAAGACGGACTCATCGACGCAATCCAAGGCTCTACCGCAATCACCATCTCAAACTCTCATTTCACTCATCATAATGATGTGATGTTATTCGGTGCAAGTGATGCATATCAAGGCGACTCGATCATGCAAGTAACCGTCGCGTTCAACCATTTTGGGAAGGGATTGGTGCAGAGAATGCCAAGATGTAGATGGGGTTTCTTCCATGTTGTCAACAATGATTACACTCACTGGCTCATGTATGCCATTGGTGGTAGTCAACATCCTACCATTATCAGTCAAGGAAACCGTTTCATTGCCCCACCCAACCCTGCTGCAAGACAGGTAAAAAATATATATATATATATATTTTAATTGGCTCTATACCATGATAGAATGAAATGAGTTAATGAGTAATGTTTCTTATGCAGATTACGAACAGAAACTATGCAACAGAGAGTGTGTGGAGAACATGGACATGGAGATCGGAAGGGGATCTGATGATGAATGGAGCGTATTTCATTCAATCTGGGAATCCAAGCAAACGAAGGCCATACAGCAGGTTCGACATGATAAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTTACACGCTTCTCTGGCTCACTCAACTGTTATATTGGTCGCCCATGCTAAAAAAACAGAACCAACAATCAAAACAAATTTCATTTACTTTCAAGCTTTAATTTAATTCAATGTTTTACCAAAAAAAAAAAAAAATTCATGGGGAAGAATAAGAGAAAGAGGATGATAGAGGATGAAGAAGTATTATGAGGAGCAGCAAAAAATGGAAGCTTTGACAAAACCAAAACCAGCAAAAGGGAAGAACAGAGAGATTCATGATCAACAACTGGAAGAAGACCTGAAGAAGAAATTTATTTATTTTTATTATTATTTTGTGTTGGAGAGAATTATTGTTGTGTTTTTCCCCTTTGCTTTGGTTATGAGAGATACAATTCCATTTTTTTTTTTTTATAAATATTTACTTTTTTATTAATCTATTTCATTCATAGTTTTGTCTTTCTCACTGTTTTTTTTTTCCTCCTTTCATTTTCCTAATAAAATTGGTTGTCTTTTTTGGATATTCAA

mRNA sequence

ATGTTGACAGCTTAGAGAAATAAAGAAAGAAAAAAAACTCAGGGTTGCTTCACATTAATTTTGGTCTGGCAAGGTCTTGAATAAACTTAGGTCTTTTACCTATTTGGGGTTCTTTCTTAGACCTTGACCAGCCCAAAAAGCTTGCTTTCAGAAAGAACCTCCAAAAATTCAAAAATAAATAAATAAATAAAAGGTCCATTTTTTTTTGCTTGGGTCTTCAAGAAGAAAAAGAAAAATGATGGCAGGCTGTTCAATTAAGTTTGTGTTGGTGTTTGTTTCAATGGCTGTTCTTATGCCAACTCTAAGAGCTGCCATTGCTGAATATGATGACTTTTTGCTCCAAAAGGCTGAAGAAGCCAAGCAAGCGTCTCTTCAAGCCTTCCATCCTGACCCCATGAATGTCACTGACCATTTCAACCAACAAGTTCATTTAGCTTTGGAGGGAATAGAGGGAAACAATAGCACTAGGAGGAGTTTGAGGAAATACAACGGTCCGTGCTTGGCGACGAATCCGATTGATCGTTGCTGGCGTTGCGACCCAAATTGGGCCAAGAACCGTAAGAAGCTTGCCACTTGTGTGCTCGGTTTCGGCCGTAAGACCACCGGTGGCAAAAGAGGACGTATCTACGTTGTGACCGATCCATCGGATAACGATGTTATTAACCCTAAACCTGGAACCCTCCGATATGCTGCTATTCAAAAACGACCATTATGGATCATTTTTGGGCGCAGTATGATCATCAGGCTATCCAAGGAGTTGTTGATAGTCAGCCACAAGACCATTGATGCTCGCGGTGCCAATGTTCACATTGCCTATGGTGCTGGTCTTAGTATTCAATTCTCAAGAAATGTGATCATCCATGGCCTTCACATCCACCACGTCATTTCAACCGGCGGTGGCATGATCCGAGATGGTGCTGACCATGTCGGACTTCGAACTGTTAGTGACGGAGACGGTATCTCCATCTTTGGATCATCCAATATCTGGCTCGATCATCTCTCCATGTCTAACTGTCAAGACGGACTCATCGACGCAATCCAAGGCTCTACCGCAATCACCATCTCAAACTCTCATTTCACTCATCATAATGATGTGATGTTATTCGGTGCAAGTGATGCATATCAAGGCGACTCGATCATGCAAGTAACCGTCGCGTTCAACCATTTTGGGAAGGGATTGGTGCAGAGAATGCCAAGATGTAGATGGGGTTTCTTCCATGTTGTCAACAATGATTACACTCACTGGCTCATGTATGCCATTGGTGGTAGTCAACATCCTACCATTATCAGTCAAGGAAACCGTTTCATTGCCCCACCCAACCCTGCTGCAAGACAGATTACGAACAGAAACTATGCAACAGAGAGTGTGTGGAGAACATGGACATGGAGATCGGAAGGGGATCTGATGATGAATGGAGCGTATTTCATTCAATCTGGGAATCCAAGCAAACGAAGGCCATACAGCAGGTTCGACATGATAAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTTACACGCTTCTCTGGCTCACTCAACTGTTATATTGGTCGCCCATGCTAAAAAAACAGAACCAACAATCAAAACAAATTTCATTTACTTTCAAGCTTTAATTTAATTCAATGTTTTACCAAAAAAAAAAAAAAATTCATGGGGAAGAATAAGAGAAAGAGGATGATAGAGGATGAAGAAGTATTATGAGGAGCAGCAAAAAATGGAAGCTTTGACAAAACCAAAACCAGCAAAAGGGAAGAACAGAGAGATTCATGATCAACAACTGGAAGAAGACCTGAAGAAGAAATTTATTTATTTTTATTATTATTTTGTGTTGGAGAGAATTATTGTTGTGTTTTTCCCCTTTGCTTTGGTTATGAGAGATACAATTCCATTTTTTTTTTTTTATAAATATTTACTTTTTTATTAATCTATTTCATTCATAGTTTTGTCTTTCTCACTGTTTTTTTTTTCCTCCTTTCATTTTCCTAATAAAATTGGTTGTCTTTTTTGGATATTCAA

Coding sequence (CDS)

ATGATGGCAGGCTGTTCAATTAAGTTTGTGTTGGTGTTTGTTTCAATGGCTGTTCTTATGCCAACTCTAAGAGCTGCCATTGCTGAATATGATGACTTTTTGCTCCAAAAGGCTGAAGAAGCCAAGCAAGCGTCTCTTCAAGCCTTCCATCCTGACCCCATGAATGTCACTGACCATTTCAACCAACAAGTTCATTTAGCTTTGGAGGGAATAGAGGGAAACAATAGCACTAGGAGGAGTTTGAGGAAATACAACGGTCCGTGCTTGGCGACGAATCCGATTGATCGTTGCTGGCGTTGCGACCCAAATTGGGCCAAGAACCGTAAGAAGCTTGCCACTTGTGTGCTCGGTTTCGGCCGTAAGACCACCGGTGGCAAAAGAGGACGTATCTACGTTGTGACCGATCCATCGGATAACGATGTTATTAACCCTAAACCTGGAACCCTCCGATATGCTGCTATTCAAAAACGACCATTATGGATCATTTTTGGGCGCAGTATGATCATCAGGCTATCCAAGGAGTTGTTGATAGTCAGCCACAAGACCATTGATGCTCGCGGTGCCAATGTTCACATTGCCTATGGTGCTGGTCTTAGTATTCAATTCTCAAGAAATGTGATCATCCATGGCCTTCACATCCACCACGTCATTTCAACCGGCGGTGGCATGATCCGAGATGGTGCTGACCATGTCGGACTTCGAACTGTTAGTGACGGAGACGGTATCTCCATCTTTGGATCATCCAATATCTGGCTCGATCATCTCTCCATGTCTAACTGTCAAGACGGACTCATCGACGCAATCCAAGGCTCTACCGCAATCACCATCTCAAACTCTCATTTCACTCATCATAATGATGTGATGTTATTCGGTGCAAGTGATGCATATCAAGGCGACTCGATCATGCAAGTAACCGTCGCGTTCAACCATTTTGGGAAGGGATTGGTGCAGAGAATGCCAAGATGTAGATGGGGTTTCTTCCATGTTGTCAACAATGATTACACTCACTGGCTCATGTATGCCATTGGTGGTAGTCAACATCCTACCATTATCAGTCAAGGAAACCGTTTCATTGCCCCACCCAACCCTGCTGCAAGACAGATTACGAACAGAAACTATGCAACAGAGAGTGTGTGGAGAACATGGACATGGAGATCGGAAGGGGATCTGATGATGAATGGAGCGTATTTCATTCAATCTGGGAATCCAAGCAAACGAAGGCCATACAGCAGGTTCGACATGATAAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTTACACGCTTCTCTGGCTCACTCAACTGTTATATTGGTCGCCCATGCTAA

Protein sequence

MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYIGRPC

Homology

[Close]

BLAST of Lsi03G014600 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.1e-164
Identity = 280/458 (61.14%), Postives = 342/458 (74.67%), Query Frame = 0


Query: 16  MAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVH---------- 75
           +AVL+PT+R  I+E D++  Q+A+EA++ +LQA+H DP  + DHF+++ +          
Sbjct: 15  IAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPE 74

Query: 76  ---------LALEGIE----GNNSTRRSLR--------KYNGPCLATNPIDRCWRCDPNW 135
                       E IE      NSTRRSLR        K  GPC A+NPID+CWRC  +W
Sbjct: 75  EDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDW 134

Query: 136 AKNRKKLATCVLGFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFG 195
           AK RKKL  CV GFG +TTGGKRGRIYVVT   D D++NPKPGTLR+A IQK PLWIIF 
Sbjct: 135 AKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFK 194

Query: 196 RSMIIRLSKELLIVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMI 255
             M IRL++ELLI SHKTIDARGANVH+A+GAG+++QF +NVIIHGLHIHH+  + GGMI
Sbjct: 195 NDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHISESSGGMI 254

Query: 256 RDGADHVGLRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHH 315
           RD  DH G+RT +DGDG+SI+GSSNIWLDH+SMS CQDGLIDAI GST ITISNSHFTHH
Sbjct: 255 RDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHH 314

Query: 316 NDVMLFGASDAYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGG 375
           NDVML GA +  + D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDYTHW +YAIGG
Sbjct: 315 NDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGG 374

Query: 376 SQHPTIISQGNRFIAPPN-PAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNP 435
           SQ PTI+S GNRFIAPP+ P  R++T R+YA+E  W+ W WRS+ D+ MNGAYF QSGNP
Sbjct: 375 SQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNP 434

Query: 436 SKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
             +  ++R  MIK K G  V +LT+++G+L+C +GR C
Sbjct: 435 QYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Lsi03G014600 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 9.9e-162
Identity = 273/463 (58.96%), Postives = 340/463 (73.43%), Query Frame = 0


Query: 14  VSMAVLMPTLRAAIA-EYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVHLALEGIE 73
           + +A L+PT+RA +A E D++ + KA EA++ +L A+HPDP  + DHF+++ +     +E
Sbjct: 13  ICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVE 72

Query: 74  G---------------------NNSTRRSL------------RKYNGPCLATNPIDRCWR 133
           G                      NSTRRSL             K  GPC A+NPID+CWR
Sbjct: 73  GTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWR 132

Query: 134 CDPNWAKNRKKLATCVLGFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPL 193
           C P+WA+ RKKL  CV GFG +TTGGKRGRIYVVT P D+D++NP+PGTLR+A IQK PL
Sbjct: 133 CQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPL 192

Query: 194 WIIFGRSMIIRLSKELLIVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVIST 253
           WI+F   M IRLS+EL+I S KTIDARGANVHIAYGAG+++Q+  N+IIHGLH+HH++ +
Sbjct: 193 WIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKS 252

Query: 254 GGGMIRDGADHVGLRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNS 313
            GG+IRD  +H G R  +DGDGISIFG++NIWLDH+SMS CQDGLIDAI GSTAITISNS
Sbjct: 253 SGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNS 312

Query: 314 HFTHHNDVMLFGASDAYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLM 373
           HFTHHNDVML GA +    D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDYTHW +
Sbjct: 313 HFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWEL 372

Query: 374 YAIGGSQHPTIISQGNRFIAPPNPA-ARQITNRNYATESVWRTWTWRSEGDLMMNGAYFI 433
           YAIGGSQ PTI+S GNRFIAPP+    R++T R+YA+ES W+ W WRSE D+ MN AYF 
Sbjct: 373 YAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFR 432

Query: 434 QSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
           QSGNP  +  +SR  MIK K G  V +LT+++G+L+C +G+ C
Sbjct: 433 QSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Lsi03G014600 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 4.6e-159
Identity = 271/450 (60.22%), Postives = 332/450 (73.78%), Query Frame = 0


Query: 2   MAGCSIKFVLVF--VSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDH 61
           M G  IK+  +F  ++ A ++P+L A I  YD+   ++AEEAK+ +   + P P NVT  
Sbjct: 1   MGGPKIKYSFLFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLA 60

Query: 62  FNQQVH---LALEGIEG--NNSTRRSL--RKYNGPCLATNPIDRCWRCDPNWAKNRKKLA 121
           FNQ++      L+ ++G  NNSTRR L  +KY GPC+ TNPID+CWRCDPNWA NRKKLA
Sbjct: 61  FNQKLRDTMKELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLA 120

Query: 122 TCVLGFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLS 181
            C +GFG K  GGK G  YVVTD SD D  +PKPGTLR+A IQK PLWIIF R M IRL 
Sbjct: 121 DCAMGFGSKAIGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLH 180

Query: 182 KELLIVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVG 241
           +E+++ S KTIDARG NVHI  GAG+++Q+ +NVIIHGLHIH ++   GGM+RD  DH+G
Sbjct: 181 QEMIMQSDKTIDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIG 240

Query: 242 LRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGA 301
           +RT SDGDGISIFG+S IW+DH+SM  C DGLIDA++GST ITISN HFT HN+VMLFGA
Sbjct: 241 IRTKSDGDGISIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGA 300

Query: 302 SDAYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIIS 361
           SD+   D +MQ+T+AFNHFGK L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HPTII 
Sbjct: 301 SDSSSIDQVMQITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIH 360

Query: 362 QGNRFIAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPS-KRRPYSR 421
           QGNRFIAPP+   +Q+T R Y  ESVW  WTWRSEG+L MNGAYF +SG+P    +    
Sbjct: 361 QGNRFIAPPDIFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDL 420

Query: 422 FDMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
           +D I A P   V  +TRF+G L C  G+PC
Sbjct: 421 YDGISAAPAEDVTWMTRFAGVLGCKPGKPC 449

[Close]

BLAST of Lsi03G014600 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.3e-158
Identity = 274/442 (61.99%), Postives = 335/442 (75.79%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           M A     + L+F + A L     A IAE+D++  +K++ A+  + +A+ P P  VT+HF
Sbjct: 1   MKAAQFFLYSLLFFASAALS---SANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYN-GPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFG 120
           N+ VH + EG    NSTRR+LR    G CLATNPIDRCWRC  NW+ NRK L  CV GFG
Sbjct: 61  NKAVHSSFEG----NSTRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFG 120

Query: 121 RKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVS 180
           RKTTGG  G IYVVTDPSD+ + +PK GTLR+  IQ RPLWIIFG+SM+IRL +EL+I +
Sbjct: 121 RKTTGGAAGEIYVVTDPSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINN 180

Query: 181 HKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDG 240
            KTID RGANV IA GA L++QF  NVIIHG+HIH +    GG+IRD   H G+RT SDG
Sbjct: 181 DKTIDGRGANVQIAGGAQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDG 240

Query: 241 DGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGD 300
           DGISI GSSNIW+DH+S++ C DGLID I GSTAITISN H T H+DVML GASD Y  D
Sbjct: 241 DGISIIGSSNIWIDHVSLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQD 300

Query: 301 SIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIA 360
            IMQVTVAFNHFG+GLVQRMPRCR+GF HVVNNDYTHW+MYA+GGSQHPTIISQGNR+IA
Sbjct: 301 EIMQVTVAFNHFGRGLVQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIA 360

Query: 361 PPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKP 420
           P   AA+++T R+YA  + W  WTW+S+GDL ++GA+F++SG P + + YS+ D+IKAKP
Sbjct: 361 PHIEAAKEVTKRDYAEPAEWSKWTWKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKP 420

Query: 421 GTFVRRLTRFSGSLNCYIGRPC 442
           GTFV+RLTRFSG+LNC     C
Sbjct: 421 GTFVQRLTRFSGALNCKENMEC 434

[Close]

BLAST of Lsi03G014600 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 5.8e-154
Identity = 260/449 (57.91%), Postives = 325/449 (72.38%), Query Frame = 0


Query: 9   FVLVFVS--MAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVHL 68
           +V VF S  +A++ P +R  +A +D +  Q+  +A + ++ ++ P P+NVT+HFN  V++
Sbjct: 11  YVFVFFSSFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNI 70

Query: 69  ALEGIEGNNSTRRSL---------RKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLG 128
           A++  E  N TRR L          K +G CLA NPID CWRCD NWA NRKKLA CVLG
Sbjct: 71  AVDASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLG 130

Query: 129 FGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLI 188
           FGR+TTGGK G IYVV D SDND+INPKPGTLR+A  +  PLWIIF RSMII+L +EL+I
Sbjct: 131 FGRRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMI 190

Query: 189 VSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVS 248
            S KTID RGA V+I  GAGL++QF  NVIIH +++ H++   GG+IRD   H+GLRT S
Sbjct: 191 TSDKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKS 250

Query: 249 DGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQ 308
           DGDGIS+FG++NIW+DH+SM+ C DG+IDAI GSTA+TISNSHFT H +VMLFGA D + 
Sbjct: 251 DGDGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHV 310

Query: 309 GDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRF 368
            D  MQ+TVAFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRF
Sbjct: 311 IDKKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRF 370

Query: 369 IAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSG-----NPSKRRPYSRF 428
           IAPPN  A+QIT R Y     W++W W+SEGD  +NGAYF+QSG     +   + P    
Sbjct: 371 IAPPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNK 430

Query: 429 DMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
             I+ KPGT VR+LT  +G L C +G  C
Sbjct: 431 FTIRPKPGTMVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of Lsi03G014600 vs. ExPASy TrEMBL
Match: A0A0A0LCU3 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 8.5e-249
Identity = 411/441 (93.20%), Postives = 428/441 (97.05%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           MM GC IK V+V VSMAVLMPTLRA IAE+DDFLLQK+EEAKQASL+AFHPDPMNVTDHF
Sbjct: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           NQ VHLALEGIEG+NSTRRSL KYNGPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGGK+GRIYVVTDPSDNDVINP+PGTLRY A+QK+PLWIIF RSMIIRLSKEL+I SH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARGANVHIAYGAGLSIQF+RNVIIHGL IHHVISTGGGMIRD A+HVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASD+YQGDS
Sbjct: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYATESVW+TWTWRSEGDLMMNGAYF+QSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNCY+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. ExPASy TrEMBL
Match: A0A1S3B8R7 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.7e-247
Identity = 408/441 (92.52%), Postives = 427/441 (96.83%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           M+AGC IK ++V VSMAVLMPTLRA IAE+DD+LLQKAEEAKQASL+AFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           NQ VHLALEGIEG+NSTRRSL KY GPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGGK+GRIYVVTDPSDNDV+NPKPGTLRY A+QKRPLWIIF RSMIIRLSKEL++ SH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQF+RNVIIHGL IHHVISTGGGMIRD A+HVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASD+YQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVW+TWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNC++GRPC
Sbjct: 421 TFVRRLTRFSGSLNCFVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. ExPASy TrEMBL
Match: A0A5A7UD78 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001200 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.4e-235
Identity = 391/422 (92.65%), Postives = 408/422 (96.68%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           M+AGC IK ++V VSMAVLMPTLRA IAE+DD+LLQKAEEAKQASL+AFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           NQ VHLALEGIEG+NSTRRSL KY GPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGGK+GRIYVVTDPSDNDV+NPKPGTLRY A+QKRPLWIIF RSMIIRLSKEL++ SH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQF+RNVIIHGL IHHVISTGGGMIRD A+HVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASD+YQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVW+TWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TF 423
           TF
Sbjct: 421 TF 422

[Close]

BLAST of Lsi03G014600 vs. ExPASy TrEMBL
Match: A0A6J1J8N7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111482295 PE=3 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.1e-235
Identity = 384/441 (87.07%), Postives = 416/441 (94.33%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           MMAG SIK  LV +SMA+LMP+LRA IAEYDDFLLQ+AEEAKQA+L+A+HP PMNVT+HF
Sbjct: 1   MMAGWSIKAALVLLSMALLMPSLRAGIAEYDDFLLQQAEEAKQAALKAYHPHPMNVTEHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           N  V+LALEGI+GNN+TRRSLRKY GPCLATNPIDRCWRCD NWA NRKKL  CVLGFGR
Sbjct: 61  NHHVYLALEGIQGNNNTRRSLRKYKGPCLATNPIDRCWRCDQNWANNRKKLGYCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGG RGR Y VTD SD DV+NPKPGTLR+A +QKRPLWIIFGRSMIIRLSKELL+ S 
Sbjct: 121 KTTGGLRGRFYTVTDASDADVVNPKPGTLRHAVVQKRPLWIIFGRSMIIRLSKELLVTSD 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARGANVHIAYGAG+SIQF+RNVIIHGLHIHH++S  GGMIRD  DHVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGISIQFTRNVIIHGLHIHHILSASGGMIRDADDHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGSSN+WLDHLSMSNCQDGLIDAIQGSTAIT+SNSHFTHHNDVMLFGASD++QGDS
Sbjct: 241 GISIFGSSNVWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSFQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAA+QITNRNYATES+W+TWTWRSEGDL+MNGAYF++SG+PSKRRPYSRFDMIKA+PG
Sbjct: 361 PNPAAKQITNRNYATESIWKTWTWRSEGDLLMNGAYFVESGDPSKRRPYSRFDMIKARPG 420

Query: 421 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNCY+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. ExPASy TrEMBL
Match: A0A6J1F1Q5 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111441594 PE=3 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 2.0e-234
Identity = 382/441 (86.62%), Postives = 416/441 (94.33%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           MMAG SIK  L+ +SMA LMP+LRA IAEYDDFLLQ+AEEAKQA+L+A+HP PMNVTDHF
Sbjct: 1   MMAGWSIKAALLLLSMAALMPSLRAGIAEYDDFLLQQAEEAKQAALKAYHPHPMNVTDHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           N  V+LAL+GI+GNNSTRRSLR+Y GPCLATNPIDRCWRCD NWA NRKKL  CVLGFGR
Sbjct: 61  NHHVYLALQGIQGNNSTRRSLRQYKGPCLATNPIDRCWRCDRNWANNRKKLGYCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGG RGR Y VTD SD DV+NPKPGTLR+A +QKRPLWIIFGRSMIIRLSKELL+ S 
Sbjct: 121 KTTGGLRGRFYTVTDASDADVVNPKPGTLRHAVVQKRPLWIIFGRSMIIRLSKELLVTSD 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARGANVHIAYGAG+SIQF+RNVIIHGLHIHH++S  GGMIRD ADHVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGISIQFARNVIIHGLHIHHILSASGGMIRDAADHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGSSN+WLDHLSMSNCQDGLIDAIQGSTAIT+SNSHFTHHNDVMLFGASD+++GDS
Sbjct: 241 GISIFGSSNVWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSFEGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           +MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 VMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAA+QITNRNYATESVW+TWTWRSEGDL+MNGAYF++SG+PSK+RPYSRFDMIKA+PG
Sbjct: 361 PNPAAKQITNRNYATESVWKTWTWRSEGDLLMNGAYFVESGDPSKQRPYSRFDMIKARPG 420

Query: 421 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNCY+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. NCBI nr
Match: XP_004147462.2 (probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_001450 [Cucumis sativus])

HSP 1 Score: 869.0 bits (2244), Expect = 1.7e-248
Identity = 411/441 (93.20%), Postives = 428/441 (97.05%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           MM GC IK V+V VSMAVLMPTLRA IAE+DDFLLQK+EEAKQASL+AFHPDPMNVTDHF
Sbjct: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           NQ VHLALEGIEG+NSTRRSL KYNGPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGGK+GRIYVVTDPSDNDVINP+PGTLRY A+QK+PLWIIF RSMIIRLSKEL+I SH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARGANVHIAYGAGLSIQF+RNVIIHGL IHHVISTGGGMIRD A+HVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASD+YQGDS
Sbjct: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYATESVW+TWTWRSEGDLMMNGAYF+QSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNCY+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. NCBI nr
Match: XP_008443432.1 (PREDICTED: probable pectate lyase 19 [Cucumis melo])

HSP 1 Score: 864.0 bits (2231), Expect = 5.6e-247
Identity = 408/441 (92.52%), Postives = 427/441 (96.83%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           M+AGC IK ++V VSMAVLMPTLRA IAE+DD+LLQKAEEAKQASL+AFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           NQ VHLALEGIEG+NSTRRSL KY GPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGGK+GRIYVVTDPSDNDV+NPKPGTLRY A+QKRPLWIIF RSMIIRLSKEL++ SH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQF+RNVIIHGL IHHVISTGGGMIRD A+HVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASD+YQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVW+TWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNC++GRPC
Sbjct: 421 TFVRRLTRFSGSLNCFVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. NCBI nr
Match: XP_038905811.1 (pectate lyase-like [Benincasa hispida])

HSP 1 Score: 857.8 bits (2215), Expect = 4.0e-245
Identity = 409/441 (92.74%), Postives = 425/441 (96.37%), Query Frame = 0


Query: 2   MAG-CSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 61
           MAG CSIK V+  VSMAVLMPTLRA IAEYDD+LLQKAEEAKQA+L++FHPDPMNVTDHF
Sbjct: 1   MAGCCSIKVVMFLVSMAVLMPTLRAGIAEYDDYLLQKAEEAKQAALESFHPDPMNVTDHF 60

Query: 62  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 121
           N+ VHLALEGIEG+NSTRRSL+KYNGPC+ATNPIDRCWRCD NWA NRKKL +CVLGFGR
Sbjct: 61  NEHVHLALEGIEGSNSTRRSLKKYNGPCMATNPIDRCWRCDRNWAMNRKKLVSCVLGFGR 120

Query: 122 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 181
           KTTGG RGR YVVTDPSDNDVINP+ GTLR+AAIQKRPLWIIFGRSMIIRLSKELLI SH
Sbjct: 121 KTTGGLRGRFYVVTDPSDNDVINPRRGTLRHAAIQKRPLWIIFGRSMIIRLSKELLITSH 180

Query: 182 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 241
           KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHV+S  GGMIRD ADHVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVVSARGGMIRDSADHVGLRTVSDGD 240

Query: 242 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 301
           GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASD+YQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 302 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 361
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 362 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 421
           PNPAARQ+TNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQVTNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 422 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNCY+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. NCBI nr
Match: KAA0053742.1 (putative pectate lyase 19 [Cucumis melo var. makuwa])

HSP 1 Score: 824.3 bits (2128), Expect = 4.9e-235
Identity = 391/422 (92.65%), Postives = 408/422 (96.68%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           M+AGC IK ++V VSMAVLMPTLRA IAE+DD+LLQKAEEAKQASL+AFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           NQ VHLALEGIEG+NSTRRSL KY GPCLATNPIDRCWRCDPNWAKNRKKLA CVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGGK+GRIYVVTDPSDNDV+NPKPGTLRY A+QKRPLWIIF RSMIIRLSKEL++ SH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQF+RNVIIHGL IHHVISTGGGMIRD A+HVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASD+YQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVW+TWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TF 423
           TF
Sbjct: 421 TF 422

[Close]

BLAST of Lsi03G014600 vs. NCBI nr
Match: XP_022983773.1 (pectate lyase-like [Cucurbita maxima])

HSP 1 Score: 823.5 bits (2126), Expect = 8.4e-235
Identity = 384/441 (87.07%), Postives = 416/441 (94.33%), Query Frame = 0


Query: 1   MMAGCSIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHF 60
           MMAG SIK  LV +SMA+LMP+LRA IAEYDDFLLQ+AEEAKQA+L+A+HP PMNVT+HF
Sbjct: 1   MMAGWSIKAALVLLSMALLMPSLRAGIAEYDDFLLQQAEEAKQAALKAYHPHPMNVTEHF 60

Query: 61  NQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLGFGR 120
           N  V+LALEGI+GNN+TRRSLRKY GPCLATNPIDRCWRCD NWA NRKKL  CVLGFGR
Sbjct: 61  NHHVYLALEGIQGNNNTRRSLRKYKGPCLATNPIDRCWRCDQNWANNRKKLGYCVLGFGR 120

Query: 121 KTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLIVSH 180
           KTTGG RGR Y VTD SD DV+NPKPGTLR+A +QKRPLWIIFGRSMIIRLSKELL+ S 
Sbjct: 121 KTTGGLRGRFYTVTDASDADVVNPKPGTLRHAVVQKRPLWIIFGRSMIIRLSKELLVTSD 180

Query: 181 KTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVSDGD 240
           KTIDARGANVHIAYGAG+SIQF+RNVIIHGLHIHH++S  GGMIRD  DHVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGISIQFTRNVIIHGLHIHHILSASGGMIRDADDHVGLRTVSDGD 240

Query: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQGDS 300
           GISIFGSSN+WLDHLSMSNCQDGLIDAIQGSTAIT+SNSHFTHHNDVMLFGASD++QGDS
Sbjct: 241 GISIFGSSNVWLDHLSMSNCQDGLIDAIQGSTAITVSNSHFTHHNDVMLFGASDSFQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAA+QITNRNYATES+W+TWTWRSEGDL+MNGAYF++SG+PSKRRPYSRFDMIKA+PG
Sbjct: 361 PNPAAKQITNRNYATESIWKTWTWRSEGDLLMNGAYFVESGDPSKRRPYSRFDMIKARPG 420

Query: 421 TFVRRLTRFSGSLNCYIGRPC 442
           TFVRRLTRFSGSLNCY+GRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

[Close]

BLAST of Lsi03G014600 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 579.7 bits (1493), Expect = 2.0e-165
Identity = 279/458 (60.92%), Postives = 342/458 (74.67%), Query Frame = 0


Query: 16  MAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVH---------- 75
           +AVL+PT+R  I+E D++  Q+A+EA++ +LQA+H DP  + DHF+++ +          
Sbjct: 15  IAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPE 74

Query: 76  ---------LALEGIE----GNNSTRRSLR--------KYNGPCLATNPIDRCWRCDPNW 135
                       E IE      NSTRRSLR        K  GPC A+NPID+CWRC  +W
Sbjct: 75  EDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDW 134

Query: 136 AKNRKKLATCVLGFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFG 195
           AK RKKL  CV GFG +TTGGKRGRIYVVT   D D++NPKPGTLR+A IQK PLWIIF 
Sbjct: 135 AKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFK 194

Query: 196 RSMIIRLSKELLIVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMI 255
             M IRL++ELLI SHKTIDARGANVH+A+GAG+++QF +NV+IHGLHIHH+  + GGMI
Sbjct: 195 NDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISESSGGMI 254

Query: 256 RDGADHVGLRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHH 315
           RD  DH G+RT +DGDG+SI+GSSNIWLDH+SMS CQDGLIDAI GST ITISNSHFTHH
Sbjct: 255 RDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHH 314

Query: 316 NDVMLFGASDAYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGG 375
           NDVML GA +  + D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDYTHW +YAIGG
Sbjct: 315 NDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGG 374

Query: 376 SQHPTIISQGNRFIAPPN-PAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNP 435
           SQ PTI+S GNRFIAPP+ P  R++T R+YA+E  W+ W WRS+ D+ MNGAYF QSGNP
Sbjct: 375 SQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNP 434

Query: 436 SKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
             +  ++R  MIK K G  V +LT+++G+L+C +GR C
Sbjct: 435 QYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Lsi03G014600 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 571.2 bits (1471), Expect = 7.0e-163
Identity = 273/463 (58.96%), Postives = 340/463 (73.43%), Query Frame = 0


Query: 14  VSMAVLMPTLRAAIA-EYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVHLALEGIE 73
           + +A L+PT+RA +A E D++ + KA EA++ +L A+HPDP  + DHF+++ +     +E
Sbjct: 13  ICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDVE 72

Query: 74  G---------------------NNSTRRSL------------RKYNGPCLATNPIDRCWR 133
           G                      NSTRRSL             K  GPC A+NPID+CWR
Sbjct: 73  GTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWR 132

Query: 134 CDPNWAKNRKKLATCVLGFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPL 193
           C P+WA+ RKKL  CV GFG +TTGGKRGRIYVVT P D+D++NP+PGTLR+A IQK PL
Sbjct: 133 CQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPL 192

Query: 194 WIIFGRSMIIRLSKELLIVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVIST 253
           WI+F   M IRLS+EL+I S KTIDARGANVHIAYGAG+++Q+  N+IIHGLH+HH++ +
Sbjct: 193 WIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKS 252

Query: 254 GGGMIRDGADHVGLRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNS 313
            GG+IRD  +H G R  +DGDGISIFG++NIWLDH+SMS CQDGLIDAI GSTAITISNS
Sbjct: 253 SGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNS 312

Query: 314 HFTHHNDVMLFGASDAYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLM 373
           HFTHHNDVML GA +    D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDYTHW +
Sbjct: 313 HFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWEL 372

Query: 374 YAIGGSQHPTIISQGNRFIAPPNPA-ARQITNRNYATESVWRTWTWRSEGDLMMNGAYFI 433
           YAIGGSQ PTI+S GNRFIAPP+    R++T R+YA+ES W+ W WRSE D+ MN AYF 
Sbjct: 373 YAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFR 432

Query: 434 QSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
           QSGNP  +  +SR  MIK K G  V +LT+++G+L+C +G+ C
Sbjct: 433 QSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Lsi03G014600 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 545.4 bits (1404), Expect = 4.1e-155
Identity = 260/449 (57.91%), Postives = 325/449 (72.38%), Query Frame = 0


Query: 9   FVLVFVS--MAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVHL 68
           +V VF S  +A++ P +R  +A +D +  Q+  +A + ++ ++ P P+NVT+HFN  V++
Sbjct: 11  YVFVFFSSFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNI 70

Query: 69  ALEGIEGNNSTRRSL---------RKYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVLG 128
           A++  E  N TRR L          K +G CLA NPID CWRCD NWA NRKKLA CVLG
Sbjct: 71  AVDASESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLG 130

Query: 129 FGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELLI 188
           FGR+TTGGK G IYVV D SDND+INPKPGTLR+A  +  PLWIIF RSMII+L +EL+I
Sbjct: 131 FGRRTTGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMI 190

Query: 189 VSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTVS 248
            S KTID RGA V+I  GAGL++QF  NVIIH +++ H++   GG+IRD   H+GLRT S
Sbjct: 191 TSDKTIDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKS 250

Query: 249 DGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAYQ 308
           DGDGIS+FG++NIW+DH+SM+ C DG+IDAI GSTA+TISNSHFT H +VMLFGA D + 
Sbjct: 251 DGDGISLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHV 310

Query: 309 GDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRF 368
            D  MQ+TVAFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRF
Sbjct: 311 IDKKMQITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRF 370

Query: 369 IAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSG-----NPSKRRPYSRF 428
           IAPPN  A+QIT R Y     W++W W+SEGD  +NGAYF+QSG     +   + P    
Sbjct: 371 IAPPNEEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNK 430

Query: 429 DMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
             I+ KPGT VR+LT  +G L C +G  C
Sbjct: 431 FTIRPKPGTMVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of Lsi03G014600 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 5.8e-149
Identity = 256/450 (56.89%), Postives = 326/450 (72.44%), Query Frame = 0


Query: 6   SIKFVLVFVSMAVLMPTLRAAIAEYDDFLLQKAEEAKQASLQAFHPDPMNVTDHFNQQVH 65
           S  FV V +S+ V++P+++A IAEYD++  Q+   A + +L+++ P+P NVTDHFN    
Sbjct: 6   SYVFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAA 65

Query: 66  LALEGIEGNNSTRRSLR---------KYNGPCLATNPIDRCWRCDPNWAKNRKKLATCVL 125
           LA+E     N TRR LR         +  G   + N ID+CWR D NW KNRKKLA CVL
Sbjct: 66  LAMETTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVL 125

Query: 126 GFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWIIFGRSMIIRLSKELL 185
           GFGRKTTGGK G IYVVTDPSDND++ PKPGT+R+A  + RPLWIIF RSMII+L +EL+
Sbjct: 126 GFGRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELI 185

Query: 186 IVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGGMIRDGADHVGLRTV 245
           I + KTID RGA ++I  GAGL++QF RNVIIH +HI  +    GG+I D   H GLRTV
Sbjct: 186 ITNDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTV 245

Query: 246 SDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDAY 305
           SDGDGI+IFG++N+W+DH+SM++C DG+IDAI GSTAITISNSHFT H++VMLFG ++  
Sbjct: 246 SDGDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKD 305

Query: 306 QGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNR 365
             D  MQ+TVAFNHFGK L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTIISQGNR
Sbjct: 306 VIDKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNR 365

Query: 366 FIAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSG-----NPSKRRPYSR 425
           FIAPP   ++Q+T R Y     W++W W+SE D  +NGAYF+QSG     + + + P  R
Sbjct: 366 FIAPPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPR 425

Query: 426 FDMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
              I+ +PGT VRRLT+ +G+L C  G+ C
Sbjct: 426 KFAIRPQPGTKVRRLTKDAGTLGCKPGKSC 455

[Close]

BLAST of Lsi03G014600 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 481.5 bits (1238), Expect = 7.3e-136
Identity = 229/399 (57.39%), Postives = 284/399 (71.18%), Query Frame = 0


Query: 43  QASLQAFHPDPMNVTDHFNQQVHLALEGIEGNNSTRRSLRKYNGPCLATNPIDRCWRCDP 102
           Q    A   DP  V D   + V ++    E N + RR L  ++  C   NPID CWRCD 
Sbjct: 79  QQQQHAVADDPDMVADEVAKLVQMS----EQNRTARRKLGFFS--CGTGNPIDDCWRCDR 138

Query: 103 NWAKNRKKLATCVLGFGRKTTGGKRGRIYVVTDPSDNDVINPKPGTLRYAAIQKRPLWII 162
           NW KNRK+LA C +GFGR   GG+ GR Y+VTDP+D DV+NPKPGTLR+A IQ+ PLWI+
Sbjct: 139 NWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIV 198

Query: 163 FGRSMIIRLSKELLIVSHKTIDARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVISTGGG 222
           F R M+I L +EL++ S KTIDARG+NVHIA GA ++IQF  NVIIHGLHIH    TG  
Sbjct: 199 FKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNA 258

Query: 223 MIRDGADHVGLRTVSDGDGISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFT 282
           M+R    H G RT++DGD +SIFGSS+IW+DH S+S+C DGL+DA+ GSTAIT+SN+HFT
Sbjct: 259 MVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFT 318

Query: 283 HHNDVMLFGASDAYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 342
           HHN+VML G SD+Y  D +MQVT+A+NHFG+GLVQRMPRCR G+FHVVNNDYTHW MYAI
Sbjct: 319 HHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAI 378

Query: 343 GGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGN 402
           GGS  PTI SQGNR+ AP +  A+++T R     S W+ W WRSEGDL++NGA+F  SG 
Sbjct: 379 GGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG- 438

Query: 403 PSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYIGRPC 442
                 Y R   + AKP + V  +T  +G+L C  GRPC
Sbjct: 439 AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:

BLAST of Lsi03G014600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFP5	2.1e-164	61.14	Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1	[more]
Q9SRH4	9.9e-162	58.96	Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1	[more]
P15722	4.6e-159	60.22	Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1	[more]
P40973	1.3e-158	61.99	Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1	[more]
Q9M9S2	5.8e-154	57.91	Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1	[more]

BLAST of Lsi03G014600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCU3	8.5e-249	93.20	Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1	[more]
A0A1S3B8R7	2.7e-247	92.52	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1	[more]
A0A5A7UD78	2.4e-235	92.65	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001200...	[more]
A0A6J1J8N7	4.1e-235	87.07	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111482295 PE=3 SV=1	[more]
A0A6J1F1Q5	2.0e-234	86.62	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111441594 PE=3 SV=1	[more]

BLAST of Lsi03G014600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147462.2	1.7e-248	93.20	probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_...	[more]
XP_008443432.1	5.6e-247	92.52	PREDICTED: probable pectate lyase 19 [Cucumis melo]	[more]
XP_038905811.1	4.0e-245	92.74	pectate lyase-like [Benincasa hispida]	[more]
KAA0053742.1	4.9e-235	92.65	putative pectate lyase 19 [Cucumis melo var. makuwa]	[more]
XP_022983773.1	8.4e-235	87.07	pectate lyase-like [Cucurbita maxima]	[more]

BLAST of Lsi03G014600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15110.1	2.0e-165	60.92	Pectate lyase family protein	[more]
AT3G01270.1	7.0e-163	58.96	Pectate lyase family protein	[more]
AT1G14420.1	4.1e-155	57.91	Pectate lyase family protein	[more]
AT2G02720.1	5.8e-149	56.89	Pectate lyase family protein	[more]
AT4G13710.1	7.3e-136	57.39	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 238..259 score: 63.42 coord: 138..163 score: 68.13 coord: 412..435 score: 42.66 coord: 114..131 score: 61.11 coord: 318..337 score: 78.1 coord: 382..406 score: 51.62 coord: 340..359 score: 79.52 coord: 174..190 score: 63.03
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 166..363 e-value: 1.2E-98 score: 343.7
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 182..357 e-value: 1.3E-18 score: 67.3
IPR007524	Pectate lyase, N-terminal	PFAM	PF04431	Pec_lyase_N	coord: 27..81 e-value: 1.2E-19 score: 70.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 91..434 e-value: 7.6E-112 score: 375.7
None	No IPR available	PANTHER	PTHR31683:SF146	PECTATE LYASE	coord: 6..441
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 6..441
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 92..431

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G014600.1	Lsi03G014600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity