Lsi03G012450 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G012450 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.4e-221
Identity = 394/576 (68.40%), Postives = 448/576 (77.78%), Query Frame = 0

Query: 4   ETVQN-DVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLS 63
           ET Q+ D+IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING   + +TY +V+L+
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELT 67

Query: 64  ARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAK 123
            RKVA GLNK+GI + D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQAK
Sbjct: 68  CRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAK 127

Query: 124 GSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEINP 183
            S +K+I+TQS +  KVK+   E  DVK++ +DS PEGCL F +L ++DE +IP V+I P
Sbjct: 128 ASSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQP 187

Query: 184 DDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYS 243
           DDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY  +EDV++CVLPLFHIYS
Sbjct: 188 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYS 247

Query: 244 LNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLS 303
           LNS+LLCGLR G AILIM KF+I   L+L++KY+VSI P VPPIVLAIAKSP ++ YDLS
Sbjct: 248 LNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLS 307

Query: 304 SIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINVQA 363
           S+R +  G APLGKELED VR KFP A LGQ                             
Sbjct: 308 SVRTVMSGAAPLGKELEDAVRTKFPNAKLGQ----------------------------- 367

Query: 364 KIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNTPG 423
                GYGMTEAGPVL M LAFAKEPF +K GACGTVVRNAEMKIVD +TGCSLPRN PG
Sbjct: 368 -----GYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPG 427

Query: 424 EICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQ 483
           EICIRGDQIMKGYLN+PEAT  TIDK+GWLHTGDIGFID+DDELFIVDRLKELIKYKGFQ
Sbjct: 428 EICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQ 487

Query: 484 VAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNS-------------EVVF 543
           VAPAE+EALLL HP ISDAAVVPMKDEQAGEVPVAFVV+   S             +V+F
Sbjct: 488 VAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIF 547

Query: 544 YKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPN 566
           YKR+ RVFF++ +PKSPSGKILRKDLRA+LAA  PN
Sbjct: 548 YKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of Lsi03G012450 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.4e-221
Identity = 403/575 (70.09%), Postives = 448/575 (77.91%), Query Frame = 0

Query: 4   ETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSA 63
           +T Q D+IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING   + +TY DV+L++
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVELNS 63

Query: 64  RKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKG 123
           RKVAAGL+K GI   D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQAK 
Sbjct: 64  RKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 123

Query: 124 SKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEINPD 183
           S +K+IVTQ+ +  KVK+   E  DVKI+ +DS PEGCL F  L +A+E DIP VEI PD
Sbjct: 124 SSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPD 183

Query: 184 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSL 243
           DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY  +EDV+LCVLPLFHIYSL
Sbjct: 184 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSL 243

Query: 244 NSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSS 303
           NSVLLCGLR G AILIM KF+I   L+L+++Y+V+I P VPPIVLAIAKSP ++ YDLSS
Sbjct: 244 NSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSS 303

Query: 304 IRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINVQAK 363
           +R +  G APLGKELEDTVRAKFP A LGQ                              
Sbjct: 304 VRTVMSGAAPLGKELEDTVRAKFPNAKLGQ------------------------------ 363

Query: 364 IFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNTPGE 423
               GYGMTEAGPVL M LAFAKEPF +K GACGTVVRNAEMKIVD +TG SLPRN  GE
Sbjct: 364 ----GYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPKTGNSLPRNQSGE 423

Query: 424 ICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQV 483
           ICIRGDQIMKGYLN+PEATA TIDK+GWL+TGDIG+IDDDDELFIVDRLKELIKYKGFQV
Sbjct: 424 ICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQV 483

Query: 484 APAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNS-------------EVVFY 543
           APAELEALLL HP ISDAAVVPMKDEQAGEVPVAFVV+   S             +V+FY
Sbjct: 484 APAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFY 542

Query: 544 KRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPN 566
           KRI RVFF+DAIPKSPSGKILRKDLRAKLAA  PN
Sbjct: 544 KRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of Lsi03G012450 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 1.0e-219
Identity = 393/578 (67.99%), Postives = 445/578 (76.99%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M  ET Q D+IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING     +TY DV+
Sbjct: 3   METETNQGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L++RKVAAGLNK+GI + D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AK S +KLI+TQ+ +  KVK+   +  ++ ++ +DS PEGC+ F +L +ADE DIP V+I
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFD-NNLNVICIDSAPEGCIHFSELTQADEHDIPDVKI 182

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
             DDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY  +EDV++CVLPLFHI
Sbjct: 183 QSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 242

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLR G AILIM KF+I    +L+EKY+V+I P VPPIVLAIAKSP ++ YD
Sbjct: 243 YSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYD 302

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSS+R +  G APLGKELED VR KFP A LGQ                           
Sbjct: 303 LSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQ--------------------------- 362

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVL M LAFAKEPF +K GACGTVVRNAEMKIVD +TGCSLPRN 
Sbjct: 363 -------GYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQ 422

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLN+P AT  TIDK+GWLHTGDIG+ID+DDELFIVDRLKELIKYKG
Sbjct: 423 PGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTGDIGYIDNDDELFIVDRLKELIKYKG 482

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNS-------------EV 540
           FQVAPAELEALLL HP ISDAAVVPMKDEQAGEVPVAFVV+   S             +V
Sbjct: 483 FQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSDITEDEVKDFVSKQV 542

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPN 566
           +FYKRI RVFF++ +PKSPSGKILRKDLRA+LAA  PN
Sbjct: 543 IFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 544

BLAST of Lsi03G012450 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 1.9e-218
Identity = 387/568 (68.13%), Postives = 450/568 (79.23%), Query Frame = 0

Query: 7   QNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKV 66
           + D+IFRSKLPDIYIPKHLPLH+YC  EN +K+GD++CLING TGE+FTY+ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  AAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKS 126
           A+GLNK+GI +GD IMLLLPNSPE+ FAFLGASY GAI T ANPFFT+AE+ KQ K S++
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQA 127

Query: 127 KLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEINPDDVV 186
           KLI+TQ+ Y +KVK+   E  +++I+ +D  P+ CL F  LM+ADE ++P V IN DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSSIRV 306
           L CGLRAG  ILIM KF+I   L+L++KY+V+I P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINVQAKIFF 366
           +  G APLGKELED VRAKFP A LGQ                                 
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQ--------------------------------- 367

Query: 367 DGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNTPGEICI 426
            GYGMTEAGPVL M LAFAKEP+ +K GACGTVVRNAEMKIVD ET  SLPRN  GEICI
Sbjct: 368 -GYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICI 427

Query: 427 RGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPA 486
           RGDQIMKGYLN+PE+T  TID++GWLHTGDIGFIDDDDELFIVDRLKE+IKYKGFQVAPA
Sbjct: 428 RGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPA 487

Query: 487 ELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLK-------------NSEVVFYKRI 546
           ELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV+               + +VVFYKRI
Sbjct: 488 ELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRI 539

Query: 547 NRVFFIDAIPKSPSGKILRKDLRAKLAA 562
            RVFF+DAIPKSPSGKILRKDLRA++A+
Sbjct: 548 FRVFFVDAIPKSPSGKILRKDLRARIAS 539

BLAST of Lsi03G012450 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 7.3e-218
Identity = 387/568 (68.13%), Postives = 449/568 (79.05%), Query Frame = 0

Query: 7   QNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKV 66
           + D+IFRSKLPDIYIPKHLPLH+YC  EN +K+GD++CLING TGE+FTY+ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  AAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKS 126
           A+GLNK+GI +GD IMLLLPNSPE+ FAFLGASY GAI T ANPFFT+AE+ KQ K S +
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127

Query: 127 KLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEINPDDVV 186
           KLI+TQ+ Y +KVK+   E  +++I+ +D  P+ CL F  LM+ADE ++P V I+ DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSSIRV 306
           L CGLRAG  ILIM KF+I   L+L++KY+V+I P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINVQAKIFF 366
           +  G APLGKELED VRAKFP A LGQ                                 
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQ--------------------------------- 367

Query: 367 DGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNTPGEICI 426
            GYGMTEAGPVL M LAFAKEP+ +K GACGTVVRNAEMKIVD ET  SLPRN  GEICI
Sbjct: 368 -GYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICI 427

Query: 427 RGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPA 486
           RGDQIMKGYLN+PE+T  TID++GWLHTGDIGFIDDDDELFIVDRLKE+IKYKGFQVAPA
Sbjct: 428 RGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPA 487

Query: 487 ELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLK-------------NSEVVFYKRI 546
           ELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV+               + +VVFYKRI
Sbjct: 488 ELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRI 539

Query: 547 NRVFFIDAIPKSPSGKILRKDLRAKLAA 562
            RVFF+DAIPKSPSGKILRKDLRAK+A+
Sbjct: 548 FRVFFVDAIPKSPSGKILRKDLRAKIAS 539

BLAST of Lsi03G012450 vs. ExPASy TrEMBL
Match: A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.8e-281
Identity = 500/580 (86.21%), Postives = 523/580 (90.17%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETV+NDVIFRSKLPDIYIP HLPLHSYCL ENAAKIG RTCLINGVTGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARKVA+GLNK+GITK DVIMLLLPNSPEFVFAFLGAS+LGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGSK+KLI+TQSSYYEK+KEITEELP+VKIMTVDSP +GCLWFGDL+KADE++IP VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAG+ ILIMPKFEIGLLL+LVEKY V++APIVPPIVLAIAKSP+LEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIRVIK GGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETG SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID+DDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDEDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE             V
Sbjct: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYK+INRVFFIDAIPKSPSGKILRK+LRAKLAAAFPNSN
Sbjct: 541 VFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN 546

BLAST of Lsi03G012450 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.4e-272
Identity = 484/580 (83.45%), Postives = 509/580 (87.76%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VTGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARKVA+GLNK+GI +GDVIML+L NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGS +KLIVTQSSYYEKVKEITEELPDVKIMTVDSPP+GCL F DL++ADE+++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAGT ILIMPKFEIG LL+LV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTE G SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTENGGSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEVPVAFVV+LKNSE             V
Sbjct: 481 FQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAFVVRLKNSEVTEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYKRI R F IDAIPKSPSGKILRK+LRAKLAA FPNSN
Sbjct: 541 VFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAAGFPNSN 546

BLAST of Lsi03G012450 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.7e-271
Identity = 482/580 (83.10%), Postives = 507/580 (87.41%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VT ESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARKVA+GLNK+GI +GDVI+LLL NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGS +KLIVTQSSYYEKVKEITEELPDVKIMTVDSPP+GCL F DL++ADE+++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAGT ILIMPKFEIG LL+LV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIRVIKCGGAPLGKELED+VRAKFPKAVLGQ                           
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTE GCSLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTENGCSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAELEALLLTHP ISDAAVVPMKDE+AGEVPVAFVV+LKNSE             V
Sbjct: 481 FQVAPAELEALLLTHPAISDAAVVPMKDEEAGEVPVAFVVRLKNSEVTEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYKRI R F IDAIPKSPSGKILRK+LRAKLA  FP SN
Sbjct: 541 VFYKRIKRAFMIDAIPKSPSGKILRKELRAKLATGFPISN 546

BLAST of Lsi03G012450 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 1.0e-262
Identity = 476/580 (82.07%), Postives = 499/580 (86.03%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M  ETV +D+IFRSKLPDIYIPKHLPLHSYCL E  A+IG RTCLIN VTGESFTY+DVD
Sbjct: 1   MANETV-HDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARK A+GL K+GI KGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGSK+KL +TQSSYYEKVKEITE+LPD KIMTVDSPP GCL F DL++  + +IP VEI
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY N+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAG  ILIMPKFEIG LL+LVEKYRVSIAPIVPPIVLAIAKSPDLEKYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAE+KIVDTE G SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICI+GDQIMKGYLNNPEATAATID +GWLHTGDIG IDDDDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIKGDQIMKGYLNNPEATAATIDNEGWLHTGDIGLIDDDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNS-------------EV 540
           FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNS             +V
Sbjct: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSKVTEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYKRINRVFFI AIPKSPSGKILRK+LRAKLAA FP  N
Sbjct: 541 VFYKRINRVFFIGAIPKSPSGKILRKELRAKLAADFPIPN 545

BLAST of Lsi03G012450 vs. ExPASy TrEMBL
Match: A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.8e-262
Identity = 477/577 (82.67%), Postives = 496/577 (85.96%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M  ETV +D IFRSKLPDIYIPKHLPLHSYCL E  A+IG RTCLIN VTGESFTY+DVD
Sbjct: 1   MATETV-HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARK A+GL K+GI KGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGSK+KLI+TQSSYYEKVKEITE+L D KIMTVDSP  GCL F DL++  +  IP VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY N+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAG  ILIMPKFEIG LL+LVEKYRVSIAPIVPPIVLAIAKSPDLEKYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAE+KIVDTE G SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATID +GWLHTGDIG IDDDDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDSEGWLHTGDIGLIDDDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE             V
Sbjct: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFP 565
           VFYKRINRVFFIDAIPKSPSGKILRK+LRAKLAA FP
Sbjct: 541 VFYKRINRVFFIDAIPKSPSGKILRKELRAKLAADFP 542

BLAST of Lsi03G012450 vs. NCBI nr
Match: XP_038904387.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.6e-289
Identity = 516/580 (88.97%), Postives = 526/580 (90.69%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD
Sbjct: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           LSARKVAAGLNK+GITK DVIMLLLPNSPEFVF FLGASYLGAIMTAANPFFTA EIAKQ
Sbjct: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGSK+KLI+T SSYYEKVKEITEELPDVKIMTVDSPP+GCLWFGDL+KADE+DIPTVEI
Sbjct: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAGT ILIMPKFEIGLLL+LVEKYRVSIAPIVPPIVLAIAKSP+LEKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETG SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDD+DELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDEDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE             V
Sbjct: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFVSKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN
Sbjct: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 546

BLAST of Lsi03G012450 vs. NCBI nr
Match: KAA0034948.1 (4-coumarate--CoA ligase 1 [Cucumis melo var. makuwa] >TYK21303.1 4-coumarate--CoA ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 976.5 bits (2523), Expect = 1.0e-280
Identity = 500/580 (86.21%), Postives = 523/580 (90.17%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETV+NDVIFRSKLPDIYIP HLPLHSYCL ENAAKIG RTCLINGVTGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARKVA+GLNK+GITK DVIMLLLPNSPEFVFAFLGAS+LGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGSK+KLI+TQSSYYEK+KEITEELP+VKIMTVDSP +GCLWFGDL+KADE++IP VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAG+ ILIMPKFEIGLLL+LVEKY V++APIVPPIVLAIAKSP+LEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIRVIK GGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETG SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID+DDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDEDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE             V
Sbjct: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYK+INRVFFIDAIPKSPSGKILRK+LRAKLAAAFPNSN
Sbjct: 541 VFYKKINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN 546

BLAST of Lsi03G012450 vs. NCBI nr
Match: XP_004137646.3 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KAE8650952.1 hypothetical protein Csa_000821 [Cucumis sativus])

HSP 1 Score: 974.5 bits (2518), Expect = 3.8e-280
Identity = 496/580 (85.52%), Postives = 521/580 (89.83%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETV+NDVIFRSKLPDIYIPKHLPLHSYCL ENAAKIG RTCLINGVTGESFTYNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ RKVA+GLNK+GITK DVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGSKSKLI+TQSSYYEKVKEITEELP+VKIMTVDSPP+GCLWFGDL+KADE+++P V+I
Sbjct: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           +P+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAG+ ILIMPKFEIGLLL+L EKY V++APIVPPIVLAIAKSP+LEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAK+PFPMKPGACGTVVRNAEMKIVDTETG SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDE+FIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE             V
Sbjct: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEASEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYKRINRVFFI+AIPKSPSGKILRK+LRAKLAAAFP SN
Sbjct: 541 VFYKRINRVFFIEAIPKSPSGKILRKELRAKLAAAFPTSN 546

BLAST of Lsi03G012450 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 947.6 bits (2448), Expect = 5.0e-272
Identity = 484/580 (83.45%), Postives = 509/580 (87.76%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VTGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARKVA+GLNK+GI +GDVIML+L NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGS +KLIVTQSSYYEKVKEITEELPDVKIMTVDSPP+GCL F DL++ADE+++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAGT ILIMPKFEIG LL+LV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTE G SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTENGGSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEVPVAFVV+LKNSE             V
Sbjct: 481 FQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAFVVRLKNSEVTEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYKRI R F IDAIPKSPSGKILRK+LRAKLAA FPNSN
Sbjct: 541 VFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAAGFPNSN 546

BLAST of Lsi03G012450 vs. NCBI nr
Match: KAG6581611.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 946.8 bits (2446), Expect = 8.5e-272
Identity = 484/580 (83.45%), Postives = 508/580 (87.59%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VTGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LSARKVAAGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           L+ARKVA+GLNK+GI +GDVIMLLL NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPEGCLWFGDLMKADEKDIPTVEI 180
           AKGS +KLIVTQSSYYEKVKEITEELPDVKIMTVDSPP+GCL F DL++ADE+++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 NPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
           NPDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAGT ILIMPKFEIG LL+LV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINV 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ                           
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQ--------------------------- 360

Query: 361 QAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNT 420
                  GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTE G SLPRNT
Sbjct: 361 -------GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTENGGSLPRNT 420

Query: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480
           PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG
Sbjct: 421 PGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKG 480

Query: 481 FQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE-------------V 540
           FQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEVPVAFVV+LKNSE             V
Sbjct: 481 FQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEVPVAFVVRLKNSEVTEDEIKQFISKQV 540

Query: 541 VFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN 568
           VFYKRI R F IDAIPKSPSGKILRK+LRAKLA  FPNSN
Sbjct: 541 VFYKRIKRAFMIDAIPKSPSGKILRKELRAKLATGFPNSN 546

BLAST of Lsi03G012450 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 722.6 bits (1864), Expect = 2.4e-208
Identity = 371/575 (64.52%), Postives = 445/575 (77.39%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVA 67
           NDVIFRS+LPDIYIP HLPLH Y + EN ++   + CLING TGE +TY DV +++RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 68  AGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKSK 127
           AGL+ +G+ + DV+M+LLPNSPE V  FL AS++GAI T+ANPFFT AEI+KQAK S +K
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 128 LIVTQSSYYEKVKEITEELPDVKIMTVDSP--PEGCLWFGDLMKADEKDIPTV--EINPD 187
           LIVTQS Y +K+K +  +   V I+T DS   PE CL F +L +++E  + ++  +I+P+
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 188 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSL 247
           DVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 248 NSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSS 307
           NS++LC LR G  ILIMPKFEI LLL+ +++ +V++A +VPPIVLAIAKSP+ EKYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 308 IRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINVQAK 367
           +R++K G APLGKELED + AKFP A LGQ                              
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQ------------------------------ 378

Query: 368 IFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNTPGE 427
               GYGMTEAGPVL M L FAKEPFP+K GACGTVVRNAEMKI+D +TG SLPRN PGE
Sbjct: 379 ----GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGE 438

Query: 428 ICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQV 487
           ICIRG+QIMKGYLN+P ATA+TIDKDGWLHTGD+GFIDDDDELFIVDRLKELIKYKGFQV
Sbjct: 439 ICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQV 498

Query: 488 APAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNS-------------EVVFY 547
           APAELE+LL+ HP I+D AVV MK+E AGEVPVAFVV+ K+S             +VVFY
Sbjct: 499 APAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFY 556

Query: 548 KRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPN 566
           KRIN+VFF D+IPK+PSGKILRKDLRA+LA    N
Sbjct: 559 KRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of Lsi03G012450 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 719.5 bits (1856), Expect = 2.0e-207
Identity = 377/574 (65.68%), Postives = 439/574 (76.48%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVA 67
           +DVIFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  AGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKSK 127
           A  +K+G+ + DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT AEIAKQAK S +K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIVTQSSYYEKVKEITEELPDVKIMTVDSP-----PEGCLWFGDLMKAD---EKDIPTVE 187
           LI+T++ Y +K+K +  +   V I+ +D       PEGCL F +L ++     + I +VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 INPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFH 247
           I+PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKY 307
           IY+LNS++LCGLR G AILIMPKFEI LLL+L+++ +V++AP+VPPIVLAIAKS + EKY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKIN 367
           DLSSIRV+K G APLGKELED V AKFP A LGQ                          
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQ-------------------------- 380

Query: 368 VQAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRN 427
                   GYGMTEAGPVL M L FAKEPFP+K GACGTVVRNAEMKIVD +TG SL RN
Sbjct: 381 --------GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRN 440

Query: 428 TPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYK 487
            PGEICIRG QIMKGYLNNP ATA TIDKDGWLHTGDIG IDDDDELFIVDRLKELIKYK
Sbjct: 441 QPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYK 500

Query: 488 GFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSE------------- 547
           GFQVAPAELEALL+ HP I+D AVV MK+E AGEVPVAFVVK K+SE             
Sbjct: 501 GFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQ 558

Query: 548 VVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLA 561
           VVFYKRIN+VFF ++IPK+PSGKILRKDLRAKLA
Sbjct: 561 VVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Lsi03G012450 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 671.4 bits (1731), Expect = 6.4e-193
Identity = 348/528 (65.91%), Postives = 407/528 (77.08%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVA 67
           +DVIFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  AGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKSK 127
           A  +K+G+ + DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT AEIAKQAK S +K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIVTQSSYYEKVKEITEELPDVKIMTVDSP-----PEGCLWFGDLMKAD---EKDIPTVE 187
           LI+T++ Y +K+K +  +   V I+ +D       PEGCL F +L ++     + I +VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 INPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFH 247
           I+PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKY 307
           IY+LNS++LCGLR G AILIMPKFEI LLL+L+++ +V++AP+VPPIVLAIAKS + EKY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKIN 367
           DLSSIRV+K G APLGKELED V AKFP A LGQ                          
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQ-------------------------- 380

Query: 368 VQAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRN 427
                   GYGMTEAGPVL M L FAKEPFP+K GACGTVVRNAEMKIVD +TG SL RN
Sbjct: 381 --------GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRN 440

Query: 428 TPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYK 487
            PGEICIRG QIMKGYLNNP ATA TIDKDGWLHTGDIG IDDDDELFIVDRLKELIKYK
Sbjct: 441 QPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYK 500

Query: 488 GFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEV 528
           GFQVAPAELEALL+ HP I+D AVV MK+E AGEVPVAFVVK K+SE+
Sbjct: 501 GFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSEL 512

BLAST of Lsi03G012450 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 651.0 bits (1678), Expect = 9.0e-187
Identity = 336/565 (59.47%), Postives = 417/565 (73.81%), Query Frame = 0

Query: 11  IFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVAAGL 70
           IFRSKLPDI IP HLPLH+YC  E  + + D+ CLI G TG+S+TY +  L  R+VA+GL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 71  NKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKSKLIV 130
            K+GI KGDVIM+LL NS EFVF+F+GAS +GA+ T ANPF+T+ E+ KQ K S +KLI+
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 131 TQSSYYEKVKEITEELPDVKIMTVDSP-PEGCLWFGDLMKADEKD--IPTVEINPDDVVA 190
           T S Y +K+K + E L    ++T D P PE CL F  L+  DE +    TV+I  DD  A
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHIYSLNSVL 250
           LP+SSGTTGLPKGV+LTHKSL+TSVAQQVDG+NPNLY  + DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 LCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSSIRVI 310
           L  LR+G  +L+M KFEIG LL L++++RV+IA +VPP+V+A+AK+P +  YDLSS+R +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 KCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKINVQAKIFFD 370
             G APLGKEL+D++R + P+A+LGQ                                  
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQ---------------------------------- 393

Query: 371 GYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRNTPGEICIR 430
           GYGMTEAGPVL+M L FAKEP P K G+CGTVVRNAE+K+V  ET  SL  N PGEICIR
Sbjct: 394 GYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIR 453

Query: 431 GDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE 490
           G QIMK YLN+PEAT+ATID++GWLHTGDIG++D+DDE+FIVDRLKE+IK+KGFQV PAE
Sbjct: 454 GQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAE 513

Query: 491 LEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNS-------------EVVFYKRIN 550
           LE+LL+ H  I+DAAVVP  DE AGEVPVAFVV+   +             +VVFYKR++
Sbjct: 514 LESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLH 560

Query: 551 RVFFIDAIPKSPSGKILRKDLRAKL 560
           +VFF+ +IPKSPSGKILRKDL+AKL
Sbjct: 574 KVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Lsi03G012450 vs. TAIR 10
Match: AT1G51680.2 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.6e-183
Identity = 331/505 (65.54%), Postives = 387/505 (76.63%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVA 67
           +DVIFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  AGLNKIGITKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKSK 127
           A  +K+G+ + DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT AEIAKQAK S +K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIVTQSSYYEKVKEITEELPDVKIMTVDSP-----PEGCLWFGDLMKAD---EKDIPTVE 187
           LI+T++ Y +K+K +  +   V I+ +D       PEGCL F +L ++     + I +VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 INPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFH 247
           I+PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGTAILIMPKFEIGLLLKLVEKYRVSIAPIVPPIVLAIAKSPDLEKY 307
           IY+LNS++LCGLR G AILIMPKFEI LLL+L+++ +V++AP+VPPIVLAIAKS + EKY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQVKSRMRYKIKRDVMKILFNWIKLKIN 367
           DLSSIRV+K G APLGKELED V AKFP A LGQ                          
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQ-------------------------- 380

Query: 368 VQAKIFFDGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGCSLPRN 427
                   GYGMTEAGPVL M L FAKEPFP+K GACGTVVRNAEMKIVD +TG SL RN
Sbjct: 381 --------GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRN 440

Query: 428 TPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDELFIVDRLKELIKYK 487
            PGEICIRG QIMKGYLNNP ATA TIDKDGWLHTGDIG IDDDDELFIVDRLKELIKYK
Sbjct: 441 QPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELIKYK 489

Query: 488 GFQVAPAELEALLLTHPVISDAAVV 505
           GFQVAPAELEALL+ HP I+D AVV
Sbjct: 501 GFQVAPAELEALLIGHPDITDVAVV 489