Lsi03G012330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G012330
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	protein N-terminal asparagine amidohydrolase isoform X1
Location	chr03: 22898698 .. 22902288 (-)
RNA-Seq Expression	Lsi03G012330
Synteny	Lsi03G012330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGCGAGGTCAATTCTGTAATCTCAATTCATAGTGATTCCCTTGGTAATGATATGAACAATAATAAAGAAATGAAAACCCATTTTCCTCTCAAACAAAGTCATTCTATAATTTCCATTGATCGGTTCCTTCTCCTTGCAGAAATTTCCCTTTCTACTCTTCTTCTCTTCCCTCTGTATTCCTTCATCTCTCACATCTCCTTCAACTAGCTTCCATACCAGAAGGTCCAGAACCATGATTTTCGTCGATGGAGCACCCTTCACCCTTGAATCATCTTCATCAAATAAGGTACTTCCCCGTCTGAATCTTCACTGTGTTGCATTTTCCCCTCTCCCCAATTTTCACTTGAAATTCCCATTTCAAACATTCTGATGAACCCCTCTAGCTTATCTTTACAGTCTCCTCCCGTTTTTCCATCGCTATAGTTGGTTTTTTGTGAAAAATGATTCAGAATTCGCCCCACTTTCTTGTTTAACGATGTGGGTGTTTTCAATCTCCTCATGGGAACATGGGTTTTCAGCTATTCAATAATATAAGTTTGTTATTGTCTTTTCTCTGATTGATCAACTTTCTGTACACACTGTTGGACTGTCAGTGTTTGACTTTTGTTGTTAAAGATTGTAATGATTCATCTGCTTATATTTGAATTTATCTGGAGGCAAATTCCTGAAAAGTAAGTCTGTTTGCCCTTGGTCATTATCATTGTTCTTGATTTTTTGATCTTGTGAACGGCACTTTTTACTGATGAAATTTCTGGATATGACTATATGAGTGGCTTGGAGAAGATATTCAATATGTATGTGACATATTGGCATCTGATGGGAAATTTTTTCTCTTAAATTAGATCACAGTCTTTAGGTATCCGAAAACCCATGCTTGGAAAAAGTTGTATGTCTTTGTTTAATCATTCAGAGAGAAATGGTTTTTCTGAACAGGGAGATGATGTCCTGTATGCCTTAATGGAGTGCCATTATCTTCTCGATGCCACAAATTCATTCAAGAGTACTCCAGAGATGATGTTCACAGTTTCTGAACAGTCCGGTGTGGAGAGATCAACTATGAGTAAATGGGTTTATGTGTTTCAGAAAGAATATGCCACTGTAGATCCTGCATTAGTAGATGTATTTTTTTTCCCTCTCCCTTTCTTTGGTTAAGCTGCTCACTAATGAATTTTAATCATGTCCTTTGCTAAATCTCTTACTGGATAATTGATATGAATAGCTTCAGAGTAACTAGAATAACTTTTACCAGCTATACTGAATTAAGCGATTGGATTAAATGGCTCAAAGATTGAATTCTGTTTTCCTGCCTTTTTATGTTTCAAAAAGTACTTTGTTAGGTACTTAAGCACCTTGGAGAACCATACCCCAAAAGCTAGCTATTGAGGTGGGAGAGCCAACCCACTTAAATACCACATTGGTCATCCCATTCTAACCAATGTGGAACAAAGGTAGCCCATACTACCTTAGTTCCTAACATAGTTGTGTTTCCAAGACGTTGTTGTGGCCAAACATCTTAGAAGAGTAGGAAGATATTAGACTCTCTTGTAGAAGTGTACACTTTTAATGAATCTATTAAAGTAAATAGTCTACCTCTCTACGATGCAGATTGCTGTTCTTTTCTAACTTTGTGTCTTTATACCCCATAATGCATAACTAAAGTTTGTTGGCACTGATGAAGCAACAACTTGTGTGGGCATAGCTATTCAGAATCGGAAAAATGGAATGTAAGAGTTAAATCTGAAAAATTATATTCTTCTGCTAATCTTGCCATTGGGTTACACTTGTCAACTTATGCATCCTAACCCTATTTCCTGGATTTCAGGACATCTGTTGCACATATGGATTTCCCAGACATAATCAAAACTGCCTTCTCACAGATGTTATCCCAAGTTGTCGATCCCGCTTCTGATGCTGAGTTAGATGTAATATCATGATGTCAATCCCCCTTATCTAACAAACTAAATGCTGTAAGCAAATGCTTGAGGACTGATTTTGAATCTATTACAAATGCAGGTTCATTTAGTAGGAGGTTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGGACCCCTAACTTTGTGATTGATTTCCAAGGATGCTATCCACAAAATCAGAATAGTTGAATTGAATTGAATGAATTGGGGTTTGATGCAGGAAAATAATAGTATCACTCGGACAGGAGATCGTAAAAAGATGGAGGGTTATTCGTTTCCACTGTGTAACAAAATTATCGAGTGTTTGTGGACAAGACCAGAGAAATTTCATCTTCAGACTCTTTGTATTCTTCAGCACAACACTCGGAGAGACTCTGAAGGGAATGCTTACCCTATCTTCAACGGATTCGTGGTCAGCCTTTGCTCCATCCAGTTTCATGTTCTGTTTGTTTTCTTCTCTCTTTTCAGGTTCAGTTGTTATTATCATCACAATTTATGAGCAGATTTACAAACTATACAATTAACATCAAACACGCCAAGAAGATCAGCATTGTTTGAGTGTGTTCGGAAAAGCAATTTTCTACCTTTAGAATAAAAATTTAATTAAAATTTGCTCGAGTAACTACTTTTAAACTTCATGTGTTTGTGAATAGTCATTTTAAGTCCTATTTTGAGATGAAAAATATACTCTTCTAAATTTAATGCAGAAGTGGATGTTGTTCTTATCCTAGTGCTACTTTTCATTTTGCTTTTTGCTAATTCAGGTGAAAACTTCTGATGGATCTGTGTTCCCTGCTAGCTTTGATAGTACTTCAAGATGTCCAGATGAAGTTGTTCGGAGAATTCGACTATCTTCATCGTACGAGGATCCACGTTGGGAGGGCAGACTGTTGGAGACATATGAAACTCAAACTGATCAGTTCAAAATCGAACCATGCTGCTGGTAAACTAATCTCCCCCATGATCTTTTTTTCCCTTTTCAAATTGTGAATATTTTGTTACAATTTACACAGCTCGACAGCGAATGATGTTCATGTGATATAATAGGTCGCCATGGAAACAACATATGGCTCTGTCTCTACAACGCCTTTCCGATTCTGAAATCCTTCAGTCATGTTCTACCTCTCCTTCTGCAGAAGGACCAGACTTCGTGGAGAATGCTAGAAGGTATCACATGTCAAGTACACTCCAAATAAGATATATTTACTCATTATATCATCTGCAATTGCCTAAATTTCAAAATGTTCTCCAGCAAATTGTATACCCCTCCCTTCCTCCAAGAATGTTACATGAGAATTTTGTCTTATACGACGCGATCTATAGTAACCAAATACCGCTCGTAACGTATGTGGAAATGAATATTCTCTCCCTCTCTAACAGGCAGTGTGCTTATTTAGTGGATCACCCAGATTGGAGACAAACCTTCCCAAAGAAGCAGCCAAGAATTTTTAGAAGGGCTGCTGATGGAAAATGGGAAAGGAGCCTCAACTGACTTCTTCTGTGCTGGTATCTTTGTATGAATCCTTGTAGTTCTGTTATCTTTAAGTGATCTGTAATATTGTTGAATATATTTCCATTTTAAATAATGAGAGAGTATATTGTTATCTTCACCCAATTAAATTTGGGTTTATGATTGGTTGTGTCTAATGAGTTTTTAG

mRNA sequence

GAGCGAGGTCAATTCTGTAATCTCAATTCATAGTGATTCCCTTGGTAATGATATGAACAATAATAAAGAAATGAAAACCCATTTTCCTCTCAAACAAAGTCATTCTATAATTTCCATTGATCGGTTCCTTCTCCTTGCAGAAATTTCCCTTTCTACTCTTCTTCTCTTCCCTCTGTATTCCTTCATCTCTCACATCTCCTTCAACTAGCTTCCATACCAGAAGGTCCAGAACCATGATTTTCGTCGATGGAGCACCCTTCACCCTTGAATCATCTTCATCAAATAAGGGAGATGATGTCCTGTATGCCTTAATGGAGTGCCATTATCTTCTCGATGCCACAAATTCATTCAAGAGTACTCCAGAGATGATGTTCACAGTTTCTGAACAGTCCGGTGTGGAGAGATCAACTATGAGTAAATGGGTTTATGTGTTTCAGAAAGAATATGCCACTGTAGATCCTGCATTAGTAGATTTTGTTGGCACTGATGAAGCAACAACTTGTGTGGGCATAGCTATTCAGAATCGGAAAAATGGAATGACATCTGTTGCACATATGGATTTCCCAGACATAATCAAAACTGCCTTCTCACAGATGTTATCCCAAGTTGTCGATCCCGCTTCTGATGCTGAGTTAGATGAAAATAATAGTATCACTCGGACAGGAGATCGTAAAAAGATGGAGGGTTATTCGTTTCCACTGTGTAACAAAATTATCGAGTGTTTGTGGACAAGACCAGAGAAATTTCATCTTCAGACTCTTTGTATTCTTCAGCACAACACTCGGAGAGACTCTGAAGGGAATGCTTACCCTATCTTCAACGGATTCGTGGTGAAAACTTCTGATGGATCTGTGTTCCCTGCTAGCTTTGATAGTACTTCAAGATGTCCAGATGAAGTTGTTCGGAGAATTCGACTATCTTCATCGTACGAGGATCCACGTTGGGAGGGCAGACTGTTGGAGACATATGAAACTCAAACTGATCAGTTCAAAATCGAACCATGCTGCTGGTCGCCATGGAAACAACATATGGCTCTGTCTCTACAACGCCTTTCCGATTCTGAAATCCTTCAGTCATGTTCTACCTCTCCTTCTGCAGAAGGACCAGACTTCGTGGAGAATGCTAGAAGGCAGTGTGCTTATTTAGTGGATCACCCAGATTGGAGACAAACCTTCCCAAAGAAGCAGCCAAGAATTTTTAGAAGGGCTGCTGATGGAAAATGGGAAAGGAGCCTCAACTGACTTCTTCTGTGCTGGTATCTTTGTATGAATCCTTGTAGTTCTGTTATCTTTAAGTGATCTGTAATATTGTTGAATATATTTCCATTTTAAATAATGAGAGAGTATATTGTTATCTTCACCCAATTAAATTTGGGTTTATGATTGGTTGTGTCTAATGAGTTTTTAG

Coding sequence (CDS)

ATGATTTTCGTCGATGGAGCACCCTTCACCCTTGAATCATCTTCATCAAATAAGGGAGATGATGTCCTGTATGCCTTAATGGAGTGCCATTATCTTCTCGATGCCACAAATTCATTCAAGAGTACTCCAGAGATGATGTTCACAGTTTCTGAACAGTCCGGTGTGGAGAGATCAACTATGAGTAAATGGGTTTATGTGTTTCAGAAAGAATATGCCACTGTAGATCCTGCATTAGTAGATTTTGTTGGCACTGATGAAGCAACAACTTGTGTGGGCATAGCTATTCAGAATCGGAAAAATGGAATGACATCTGTTGCACATATGGATTTCCCAGACATAATCAAAACTGCCTTCTCACAGATGTTATCCCAAGTTGTCGATCCCGCTTCTGATGCTGAGTTAGATGAAAATAATAGTATCACTCGGACAGGAGATCGTAAAAAGATGGAGGGTTATTCGTTTCCACTGTGTAACAAAATTATCGAGTGTTTGTGGACAAGACCAGAGAAATTTCATCTTCAGACTCTTTGTATTCTTCAGCACAACACTCGGAGAGACTCTGAAGGGAATGCTTACCCTATCTTCAACGGATTCGTGGTGAAAACTTCTGATGGATCTGTGTTCCCTGCTAGCTTTGATAGTACTTCAAGATGTCCAGATGAAGTTGTTCGGAGAATTCGACTATCTTCATCGTACGAGGATCCACGTTGGGAGGGCAGACTGTTGGAGACATATGAAACTCAAACTGATCAGTTCAAAATCGAACCATGCTGCTGGTCGCCATGGAAACAACATATGGCTCTGTCTCTACAACGCCTTTCCGATTCTGAAATCCTTCAGTCATGTTCTACCTCTCCTTCTGCAGAAGGACCAGACTTCGTGGAGAATGCTAGAAGGCAGTGTGCTTATTTAGTGGATCACCCAGATTGGAGACAAACCTTCCCAAAGAAGCAGCCAAGAATTTTTAGAAGGGCTGCTGATGGAAAATGGGAAAGGAGCCTCAACTGA

Protein sequence

MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTMSKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQMLSQVVDPASDAELDENNSITRTGDRKKMEGYSFPLCNKIIECLWTRPEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPSAEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN

Homology

[Close]

BLAST of Lsi03G012330 vs. ExPASy Swiss-Prot
Match: O64876 (Protein N-terminal asparagine amidohydrolase OS=Arabidopsis thaliana OX=3702 GN=NTAN1 PE=2 SV=2)

HSP 1 Score: 355.9 bits (912), Expect = 5.0e-97
Identity = 181/347 (52.16%), Postives = 237/347 (68.30%), Query Frame = 0


Query: 1   MIFVDGAPFTLES-----SSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGV 60
           MI+V G  F  ES     SSS++G  +L  +M    +  A++SFK+  E   +  E S  
Sbjct: 1   MIYVGGVQFLDESSSFSLSSSSQGSSLLVDVMSHPVITLASDSFKNLEEKNVSFDE-SDS 60

Query: 61  ERSTMSKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIK 120
           E ST  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+ I+NRK+GMTSVAHMD P+I+ 
Sbjct: 61  ESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNRKSGMTSVAHMDSPEIVD 120

Query: 121 TAFSQMLSQVVDPASDAELD----------ENNSITRTGDRKKMEGYSFPLCNKIIECLW 180
              SQML  V+    DAELD          +  +    GD  K EGYSFPLC K++E L 
Sbjct: 121 LGISQMLLLVLQDDVDAELDVHMVGGYEDVDIKNADGVGDYAKPEGYSFPLCCKLVETLQ 180

Query: 181 TRPEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRR 240
            R E FH+QTL IL HNT+ DS+ N  PIFNG +V TS G++ PASF+ TSRCPDE+VRR
Sbjct: 181 KRRENFHIQTLFILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNRTSRCPDEIVRR 240

Query: 241 IRLSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTS 300
           IR+SSS+ED  W+G+LL+TY+T+TD+F I PC W+         L +L+D EIL +CSTS
Sbjct: 241 IRVSSSFEDSSWKGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLTDEEILTNCSTS 300

Query: 301 PSAEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWER 333
           PSAEGPDFV + RR   YL+ +P+W +TFP++QPR+F R  DG W++
Sbjct: 301 PSAEGPDFVNSLRRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346

[Close]

BLAST of Lsi03G012330 vs. ExPASy Swiss-Prot
Match: Q64311 (Protein N-terminal asparagine amidohydrolase OS=Mus musculus OX=10090 GN=Ntan1 PE=1 SV=3)

HSP 1 Score: 117.9 bits (294), Expect = 2.3e-25
Identity = 82/274 (29.93%), Postives = 138/274 (50.36%), Query Frame = 0


Query: 64  VYVFQKEYATVDP--ALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQM 123
           +YV Q+E A   P    +  +G+D+ATTC  + +++  NG T + H D  D  K     +
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGSD-TKAEVPLI 106

Query: 124 LSQVVDPASDAELD--ENNSITRTGDRKKMEGYSFPLCNKIIECLWTRPEKFHLQTLCIL 183
           +S +   +  AE    E + +    D +++   S  L ++++     + +  HL TLC+ 
Sbjct: 107 MSSIKSFSEHAECGRLEVHLVGGFSDDRQL---SQKLTHQLLSEFDKQDDDIHLVTLCVT 166

Query: 184 QHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPRWEG 243
           + N R ++E N +PI  G  V      ++ ASF    R P+E +R  R  +        G
Sbjct: 167 ELNDREENE-NHFPIIYGIAVNIKTAEIYRASFQ--DRGPEEQLRAARALAG-------G 226

Query: 244 RLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPSAEGPDFVENARR 303
            ++  Y+ +T+Q +I PC W+P+ Q +   LQ+  D +IL+S STSP AE P FVE+ R 
Sbjct: 227 PMISIYDAKTEQLRIGPCSWTPFPQ-VDFWLQQ-DDKQILESLSTSPLAEPPHFVEHIRS 286

Query: 304 QCAYLVDHPDWRQ-TFPKKQPRIFRRAADGKWER 333
              +L   P      FP  +  ++++  DG WE+
Sbjct: 287 TLMFLKKFPSPENILFPGNKALLYKKNKDGLWEK 304

[Close]

BLAST of Lsi03G012330 vs. ExPASy Swiss-Prot
Match: Q28955 (Protein N-terminal asparagine amidohydrolase OS=Sus scrofa OX=9823 GN=NTAN1 PE=1 SV=4)

HSP 1 Score: 114.0 bits (284), Expect = 3.3e-24
Identity = 81/271 (29.89%), Postives = 133/271 (49.08%), Query Frame = 0


Query: 64  VYVFQKEYATVDP--ALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQM 123
           +YV Q+E A   P    V  +G+D+ATTC  + +++  NG T + H D  D  K   S +
Sbjct: 47  LYVQQRELAVTSPKDGSVCILGSDDATTCHIVVLRHTGNGATCLTHCDGTD-TKAEVSLI 106

Query: 124 LSQVVDPASDAELDENNSITRTGDRKKMEGYSFPLCNKIIECLWTRPEKFHLQTLCILQH 183
           +S  +   SD        +   G        S  L ++++     + +  HL TLC+ + 
Sbjct: 107 MSS-IKSFSDHTQRGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTEL 166

Query: 184 NTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPRWEGRL 243
           N R ++E N +PI  G  V      ++ ASF    R P+E +R  R+ +        G +
Sbjct: 167 NDREENE-NHFPIIYGIAVNVKTAEIYRASF--PDRGPEEELRAARVLTG-------GPM 226

Query: 244 LETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPSAEGPDFVENARRQC 303
           +  Y+ +T+Q +I P  W P+  H+   LQ+  D +IL++ STSP AE P FVE+ R   
Sbjct: 227 ISIYDAKTEQLRIGPYSWMPF-PHVDFWLQQ-DDKQILENLSTSPLAEPPHFVEHIRSTL 286

Query: 304 AYLVDHPDWRQT-FPKKQPRIFRRAADGKWE 332
            +L  +P    T FP  +  ++++  DG W+
Sbjct: 287 MFLKKYPSPTNTLFPGNKALLYKKNEDGLWK 303

[Close]

BLAST of Lsi03G012330 vs. ExPASy Swiss-Prot
Match: Q96AB6 (Protein N-terminal asparagine amidohydrolase OS=Homo sapiens OX=9606 GN=NTAN1 PE=1 SV=3)

HSP 1 Score: 112.5 bits (280), Expect = 9.5e-24
Identity = 77/272 (28.31%), Postives = 130/272 (47.79%), Query Frame = 0


Query: 64  VYVFQKEYATVDP--ALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQM 123
           +YV Q+E A   P    +  +G+D+ATTC  + +++  NG T + H D  D    A   +
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTD--TKAEVPL 106

Query: 124 LSQVVDPASDAELDENNSITRTGDRKKMEGYSFPLCNKIIECLWTRPEKFHLQTLCILQH 183
           +   +   SD        +   G        S  L ++++     + +  HL TLC+ + 
Sbjct: 107 IMNSIKSFSDHAQCGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTEL 166

Query: 184 NTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPRWEGRL 243
           N R ++E N +P+  G  V      ++ ASF    R P+E +R  R  +        G +
Sbjct: 167 NDREENE-NHFPVIYGIAVNIKTAEIYRASFQ--DRGPEEQLRAARTLAG-------GPM 226

Query: 244 LETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPSAEGPDFVENARRQC 303
           +  Y+ +T+Q +I P  W+P+  H+   L +  D +IL++ STSP AE P FVE+ R   
Sbjct: 227 ISIYDAETEQLRIGPYSWTPF-PHVDFWLHQ-DDKQILENLSTSPLAEPPHFVEHIRSTL 286

Query: 304 AYLVDHPDWRQT-FPKKQPRIFRRAADGKWER 333
            +L  HP    T F   +  ++++  DG WE+
Sbjct: 287 MFLKKHPSPAHTLFSGNKALLYKKNEDGLWEK 304

[Close]

BLAST of Lsi03G012330 vs. ExPASy TrEMBL
Match: A0A1S3CFG9 (protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500294 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.3e-172
Identity = 299/348 (85.92%), Postives = 313/348 (89.94%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDGAPFTL+SSSSNKG DVLYALMEC YL+DATN FK TPE+ FTVSE+SGVERSTM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRFTVSEESGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAI+NRKNGMTSVAHMDFPDII  A SQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIINIALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP +DAELD             ENN+ITRTGDRKKMEGYS PLCNKII  LWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PEKFHLQTLCIL HNTRRDS GN+YPIFNGF VKTSDGSVFPASFDSTSRCPDEVVRRIR
Sbjct: 181 PEKFHLQTLCILLHNTRRDSHGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEVVRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP WEGRLLETYETQTDQFKIEPC W+PWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFKIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
            EGPDFVEN+RRQ AYLV+HPDWR+TFPKK+ RIFRRAA+GKWERS N
Sbjct: 301 VEGPDFVENSRRQWAYLVEHPDWRETFPKKKARIFRRAANGKWERSFN 348

[Close]

BLAST of Lsi03G012330 vs. ExPASy TrEMBL
Match: A0A0A0L9W7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G702610 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 296/346 (85.55%), Postives = 313/346 (90.46%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDGAPFTL+SSSSNKG DVLYALMEC YL+DATN FK TPE+  TVSE+SGVER TM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRVTVSEESGVERPTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAI+NRKNG+TSVAHMDFPDII+ A SQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGITSVAHMDFPDIIQIALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP +DAELD             ENN+ITR GDRKKMEGYS PLCNKII  LWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PEKFHLQTLCILQHNTRRDSEGN+YPIFNGF VKTSDGSVFPASFDSTSRCPDE+VRRIR
Sbjct: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP WEGRLLETYETQTDQF+IEPC W+PWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERS 334
            EGPDFVENARRQ AYLV+HPDWR+TFPKK+PRIFRRAA+GKWERS
Sbjct: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERS 346

[Close]

BLAST of Lsi03G012330 vs. ExPASy TrEMBL
Match: A0A6J1FB23 (protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442418 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 282/348 (81.03%), Postives = 308/348 (88.51%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDG PFT+ESSSSNKG DVLYALMEC +L+DAT SFKS PEM FT SEQ GVERSTM
Sbjct: 1   MIFVDGVPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
            KWVY+FQKEYATVDPALVDFVGTDEATTCVG+AI+NRKNGMTSVAH+DFP+II+ A SQ
Sbjct: 61  CKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP+SDAELD             E+N IT   DRKK EG+SFPLCNKIIE LWTR
Sbjct: 121 MLSLVVDPSSDAELDVHLVGGFEDVLLKESNDITELEDRKKTEGHSFPLCNKIIERLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PE FHLQTLC+L HNTRRDSEGNAYPIF+GF VKTSDGSVFPASFDSTSRCPDEV+RRIR
Sbjct: 181 PETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP  +GRLLETYET TDQFKIEPCCWSPWK++MALSLQ+LSD+EILQSCSTSPS
Sbjct: 241 LSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
           AE PDFVEN+RRQ AYLV+HPDWR++FP+K+PRIFRRAADGKWERS N
Sbjct: 301 AEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 348

[Close]

BLAST of Lsi03G012330 vs. ExPASy TrEMBL
Match: A0A5A7U4D2 (Protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00060 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.3e-161
Identity = 282/331 (85.20%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 18  KGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTMSKWVYVFQKEYATVDPA 77
           +G DVLYALMEC YL+DATN FK TPE+ FTVSE+SGVERSTMSKWVYVFQKEYATVDPA
Sbjct: 7   QGADVLYALMECPYLVDATNLFKGTPEIRFTVSEESGVERSTMSKWVYVFQKEYATVDPA 66

Query: 78  LVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQMLSQVVDPASDAELD-- 137
           LVDFVGTDEATTCVGIAI+NRKNGMTSVAHMDFPDII  A SQMLS VVDP +DAELD  
Sbjct: 67  LVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIINIALSQMLSLVVDPTADAELDVH 126

Query: 138 -----------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTRPEKFHLQTLCILQHNTR 197
                      ENN+ITRTGDRKKMEGYS PLCNKII  LWTRPEKFHLQTLCIL HNTR
Sbjct: 127 LVGGFEDVLLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTR 186

Query: 198 RDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPRWEGRLLET 257
           RDS GN+YPIFNGF VKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDP WEGRLLET
Sbjct: 187 RDSHGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWEGRLLET 246

Query: 258 YETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPSAEGPDFVENARRQCAYL 317
           YETQTDQFKIEPC W+PWKQHMALSLQRLSDSEILQSCSTSPS EGPDFVEN+RRQ AYL
Sbjct: 247 YETQTDQFKIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPSVEGPDFVENSRRQWAYL 306

Query: 318 VDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
           V+HPDWR+TFPKK+ RIFRRAA+GKWERS N
Sbjct: 307 VEHPDWRETFPKKKARIFRRAANGKWERSFN 337

[Close]

BLAST of Lsi03G012330 vs. ExPASy TrEMBL
Match: A0A6J1J4Y8 (protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481410 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.7e-161
Identity = 280/348 (80.46%), Postives = 307/348 (88.22%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDG PFT+ESSSSNKG DVLYALMEC +L+DAT SFKS PEM FT SEQ GVERST+
Sbjct: 49  MIFVDGIPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTI 108

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
            KWVYVFQKEYATVDPALVDFVGTDEATTCVG+A +NR+NGMTSVAH+DFP+II+ A SQ
Sbjct: 109 CKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQ 168

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP+SDAELD             E+N IT   DRKK EGYSFPLCNKIIE LWTR
Sbjct: 169 MLSLVVDPSSDAELDVHLVGGFEDVLLKESNDITELEDRKKTEGYSFPLCNKIIERLWTR 228

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PE FHLQTLC+L HNTRRDS+GNAYPIF+GF VKTSDGSVFPASFDSTSRCPDEV+RRIR
Sbjct: 229 PETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIR 288

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP  +GRLLETYET TDQFKIEPCCWSPWK++MALSLQ+LSD+EILQSCSTSPS
Sbjct: 289 LSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPS 348

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
           AE PDFVEN+RRQ AYLV+HPDW+++FPKK+PRIFRRAADGKWERS N
Sbjct: 349 AEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 396

[Close]

BLAST of Lsi03G012330 vs. NCBI nr
Match: XP_038906106.1 (protein N-terminal asparagine amidohydrolase [Benincasa hispida])

HSP 1 Score: 626.3 bits (1614), Expect = 1.5e-175
Identity = 306/348 (87.93%), Postives = 319/348 (91.67%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDGAPFT+ESSSSNKG DVLYALMECHYL++ATNSFK TPEM FTVSEQSGVERSTM
Sbjct: 1   MIFVDGAPFTVESSSSNKGADVLYALMECHYLVNATNSFKGTPEMRFTVSEQSGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
           SKW+YVFQKEYA VDPALVDFVGTDEATTCVGIAI+NRKNGMTSVAHMDFPDIIK A SQ
Sbjct: 61  SKWIYVFQKEYAIVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIKIALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDPASD ELD             ENN+ITRTGD KKMEGYSFPLCNKIIE LWTR
Sbjct: 121 MLSLVVDPASDVELDVHLVGGFEDVLLEENNNITRTGDHKKMEGYSFPLCNKIIESLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PEKFHLQTLCIL+HNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR
Sbjct: 181 PEKFHLQTLCILRHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP WEGRLLETYETQTDQFKIEPC WSPWKQ +ALSLQ LSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFKIEPCRWSPWKQRIALSLQCLSDSEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
           AEGPDFVE+ARRQ AYLV+HPDWR+TFPKK+PRIFRRAADGKWER+ N
Sbjct: 301 AEGPDFVESARRQWAYLVEHPDWRETFPKKKPRIFRRAADGKWERNFN 348

[Close]

BLAST of Lsi03G012330 vs. NCBI nr
Match: XP_008461769.1 (PREDICTED: protein N-terminal asparagine amidohydrolase isoform X1 [Cucumis melo])

HSP 1 Score: 612.8 bits (1579), Expect = 1.7e-171
Identity = 299/348 (85.92%), Postives = 313/348 (89.94%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDGAPFTL+SSSSNKG DVLYALMEC YL+DATN FK TPE+ FTVSE+SGVERSTM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRFTVSEESGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAI+NRKNGMTSVAHMDFPDII  A SQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIINIALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP +DAELD             ENN+ITRTGDRKKMEGYS PLCNKII  LWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PEKFHLQTLCIL HNTRRDS GN+YPIFNGF VKTSDGSVFPASFDSTSRCPDEVVRRIR
Sbjct: 181 PEKFHLQTLCILLHNTRRDSHGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEVVRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP WEGRLLETYETQTDQFKIEPC W+PWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFKIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
            EGPDFVEN+RRQ AYLV+HPDWR+TFPKK+ RIFRRAA+GKWERS N
Sbjct: 301 VEGPDFVENSRRQWAYLVEHPDWRETFPKKKARIFRRAANGKWERSFN 348

[Close]

BLAST of Lsi03G012330 vs. NCBI nr
Match: XP_004149611.1 (protein N-terminal asparagine amidohydrolase [Cucumis sativus] >KGN58623.1 hypothetical protein Csa_001991 [Cucumis sativus])

HSP 1 Score: 608.6 bits (1568), Expect = 3.2e-170
Identity = 296/346 (85.55%), Postives = 313/346 (90.46%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDGAPFTL+SSSSNKG DVLYALMEC YL+DATN FK TPE+  TVSE+SGVER TM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRVTVSEESGVERPTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAI+NRKNG+TSVAHMDFPDII+ A SQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGITSVAHMDFPDIIQIALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP +DAELD             ENN+ITR GDRKKMEGYS PLCNKII  LWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PEKFHLQTLCILQHNTRRDSEGN+YPIFNGF VKTSDGSVFPASFDSTSRCPDE+VRRIR
Sbjct: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP WEGRLLETYETQTDQF+IEPC W+PWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERS 334
            EGPDFVENARRQ AYLV+HPDWR+TFPKK+PRIFRRAA+GKWERS
Sbjct: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERS 346

[Close]

BLAST of Lsi03G012330 vs. NCBI nr
Match: XP_022935590.1 (protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita moschata] >KAG6581602.1 Protein N-terminal asparagine amidohydrolase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 581.6 bits (1498), Expect = 4.2e-162
Identity = 282/348 (81.03%), Postives = 308/348 (88.51%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDG PFT+ESSSSNKG DVLYALMEC +L+DAT SFKS PEM FT SEQ GVERSTM
Sbjct: 1   MIFVDGVPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
            KWVY+FQKEYATVDPALVDFVGTDEATTCVG+AI+NRKNGMTSVAH+DFP+II+ A SQ
Sbjct: 61  CKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP+SDAELD             E+N IT   DRKK EG+SFPLCNKIIE LWTR
Sbjct: 121 MLSLVVDPSSDAELDVHLVGGFEDVLLKESNDITELEDRKKTEGHSFPLCNKIIERLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PE FHLQTLC+L HNTRRDSEGNAYPIF+GF VKTSDGSVFPASFDSTSRCPDEV+RRIR
Sbjct: 181 PETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP  +GRLLETYET TDQFKIEPCCWSPWK++MALSLQ+LSD+EILQSCSTSPS
Sbjct: 241 LSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
           AE PDFVEN+RRQ AYLV+HPDWR++FP+K+PRIFRRAADGKWERS N
Sbjct: 301 AEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 348

[Close]

BLAST of Lsi03G012330 vs. NCBI nr
Match: XP_023529134.1 (protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579.3 bits (1492), Expect = 2.1e-161
Identity = 281/348 (80.75%), Postives = 306/348 (87.93%), Query Frame = 0


Query: 1   MIFVDGAPFTLESSSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGVERSTM 60
           MIFVDG PFT+ESSSSNKG DVLYALMEC +L+DAT SFKS PEM FT SEQ GVERSTM
Sbjct: 1   MIFVDGVPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIKTAFSQ 120
            KWVY+FQKEYATVDPALVDFVGTDEATTCVG+AI+NRKNGMTSVAH+DFP+II+ A SQ
Sbjct: 61  CKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQ 120

Query: 121 MLSQVVDPASDAELD-------------ENNSITRTGDRKKMEGYSFPLCNKIIECLWTR 180
           MLS VVDP+SDAELD             E+N I    DRKK EG+SFPLCNKIIECLWTR
Sbjct: 121 MLSLVVDPSSDAELDVHLVGGFEDVLLQESNDIPELEDRKKTEGHSFPLCNKIIECLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240
           PE FHLQTLC+L HNT RDSEGNAYPIF+GF VKTSDGSVFPASFDSTSRCPDEV+RRIR
Sbjct: 181 PETFHLQTLCVLHHNTWRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIR 240

Query: 241 LSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP  +GRLLETYET TDQFKIEPCCWSPWK++MALSLQ+LSD+EILQSCSTSPS
Sbjct: 241 LSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPS 300

Query: 301 AEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWERSLN 336
           AE PDFVEN+RRQ AYLV+H DWR++FPKK+PRIFRRAADGKWERS N
Sbjct: 301 AEAPDFVENSRRQWAYLVEHSDWRESFPKKKPRIFRRAADGKWERSFN 348

[Close]

BLAST of Lsi03G012330 vs. TAIR 10
Match: AT2G44420.1 (protein N-terminal asparagine amidohydrolase family protein )

HSP 1 Score: 355.9 bits (912), Expect = 3.5e-98
Identity = 181/347 (52.16%), Postives = 237/347 (68.30%), Query Frame = 0


Query: 1   MIFVDGAPFTLES-----SSSNKGDDVLYALMECHYLLDATNSFKSTPEMMFTVSEQSGV 60
           MI+V G  F  ES     SSS++G  +L  +M    +  A++SFK+  E   +  E S  
Sbjct: 1   MIYVGGVQFLDESSSFSLSSSSQGSSLLVDVMSHPVITLASDSFKNLEEKNVSFDE-SDS 60

Query: 61  ERSTMSKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIQNRKNGMTSVAHMDFPDIIK 120
           E ST  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+ I+NRK+GMTSVAHMD P+I+ 
Sbjct: 61  ESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNRKSGMTSVAHMDSPEIVD 120

Query: 121 TAFSQMLSQVVDPASDAELD----------ENNSITRTGDRKKMEGYSFPLCNKIIECLW 180
              SQML  V+    DAELD          +  +    GD  K EGYSFPLC K++E L 
Sbjct: 121 LGISQMLLLVLQDDVDAELDVHMVGGYEDVDIKNADGVGDYAKPEGYSFPLCCKLVETLQ 180

Query: 181 TRPEKFHLQTLCILQHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRR 240
            R E FH+QTL IL HNT+ DS+ N  PIFNG +V TS G++ PASF+ TSRCPDE+VRR
Sbjct: 181 KRRENFHIQTLFILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNRTSRCPDEIVRR 240

Query: 241 IRLSSSYEDPRWEGRLLETYETQTDQFKIEPCCWSPWKQHMALSLQRLSDSEILQSCSTS 300
           IR+SSS+ED  W+G+LL+TY+T+TD+F I PC W+         L +L+D EIL +CSTS
Sbjct: 241 IRVSSSFEDSSWKGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLTDEEILTNCSTS 300

Query: 301 PSAEGPDFVENARRQCAYLVDHPDWRQTFPKKQPRIFRRAADGKWER 333
           PSAEGPDFV + RR   YL+ +P+W +TFP++QPR+F R  DG W++
Sbjct: 301 PSAEGPDFVNSLRRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346

The following BLAST results are available for this feature:

BLAST of Lsi03G012330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64876	5.0e-97	52.16	Protein N-terminal asparagine amidohydrolase OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q64311	2.3e-25	29.93	Protein N-terminal asparagine amidohydrolase OS=Mus musculus OX=10090 GN=Ntan1 P...	[more]
Q28955	3.3e-24	29.89	Protein N-terminal asparagine amidohydrolase OS=Sus scrofa OX=9823 GN=NTAN1 PE=1...	[more]
Q96AB6	9.5e-24	28.31	Protein N-terminal asparagine amidohydrolase OS=Homo sapiens OX=9606 GN=NTAN1 PE...	[more]

BLAST of Lsi03G012330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CFG9	8.3e-172	85.92	protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucumis melo OX=3656 ...	[more]
A0A0A0L9W7	1.6e-170	85.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G702610 PE=4 SV=1	[more]
A0A6J1FB23	2.0e-162	81.03	protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita moschata OX...	[more]
A0A5A7U4D2	1.3e-161	85.20	Protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucumis melo var. mak...	[more]
A0A6J1J4Y8	1.7e-161	80.46	protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita maxima OX=3...	[more]

BLAST of Lsi03G012330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906106.1	1.5e-175	87.93	protein N-terminal asparagine amidohydrolase [Benincasa hispida]	[more]
XP_008461769.1	1.7e-171	85.92	PREDICTED: protein N-terminal asparagine amidohydrolase isoform X1 [Cucumis melo...	[more]
XP_004149611.1	3.2e-170	85.55	protein N-terminal asparagine amidohydrolase [Cucumis sativus] >KGN58623.1 hypot...	[more]
XP_022935590.1	4.2e-162	81.03	protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita moschata] >KA...	[more]
XP_023529134.1	2.1e-161	80.75	protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita pepo subsp. p...	[more]

BLAST of Lsi03G012330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44420.1	3.5e-98	52.16	protein N-terminal asparagine amidohydrolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026750	Protein N-terminal asparagine amidohydrolase	PFAM	PF14736	N_Asn_amidohyd	coord: 56..332 e-value: 7.8E-85 score: 284.3
IPR026750	Protein N-terminal asparagine amidohydrolase	PANTHER	PTHR12498	N-TERMINAL ASPARAGINE AMIDOHYDROLASE	coord: 1..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G012330.1	Lsi03G012330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008418	protein-N-terminal asparagine amidohydrolase activity