Lsi03G010850 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G010850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	bifunctional epoxide hydrolase 2-like
Location	chr03: 20747176 .. 20749327 (-)
RNA-Seq Expression	Lsi03G010850
Synteny	Lsi03G010850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAAAGTTGATTGTTCCATAACGCCAATGATATCAATTTCGAATTGGCTTAAAATTCCCCTTCTTTCTATTTAAACAAACCCACCACTCAACGAATTCCCAAACACACACAACCAATGGACACCATCCACCACACCACCGTTCAAACCAACGGCATCAAACTCCACGTCGCCTCCGCCGGCCCTGTAACCGGCCCACCCGTGCTCCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCCTGGCGCCACCAGATCGTTTTCCTCTCCTCCGTCGGGTACCGAGTCATCGCCCCGGACCTCCGCGGCTACGGTGACTCGGACGCGCCGCCGTCCGCCGAGTCATACACCACCCTGCACATCGTGGGGGATTTGGTGGGGCTGTTGGATGAGCTGGGGATTGAGAAGGTGTTATTGGTGGGCCATGATTGGGGAGCCTTGATTGCTTGGTACTTTTGCTTGTTTAGGCCGGATAAGATCAAGGCGTCGGTGATTTTGAGCGTCCAGTTTTTTCCTAGAAACCCAACAACGCCATTTATAGAAGGCTTCAAAGCTGTTCTTGGAGACCAATTTTATATGGTCAGATTTCAGGTCGTTTTCTAACCATATCCTCCATTTAGTTTTCAACAGTTTTGATCTTAGCTAAAGAGCTGAAAAAAGCTGTTCCTCTATGTTATATGCTATCATAGATTCTTCTAGTTATGTTTGGAAATTAGTGTAGTACAAATTCAAACAGTATTGATGCGACGGAAAATATTAAAAAATAAAAATAAATTTAAAGTTAAATCAGGTCTGTTTGATTCGCTTACTTCTTAAGTTGTGTAAATGTTAGATATCATATAATTTATCTTCTTCCGTTAGCTTAAAATTTTAGGTCAATCGACGATTTAAGATGATACTTTTAGTGGTTCAGGGAGGTTCGGTGTTCAGGTCCCTACAATATTATTTTCACATTTATCGAGCAACTCCTTCTTTCTCTCTATCTTTATATTTCATCTTTCTCAAGTAAATTCATCTTCTTCTCTTTATCTACTTTTCATAACTATTGAAAAACTCTAGTTTACAACTCTACAACTCAAATACAAATTTACTAACTCCAACAATTTAATCTTAAAGAAATTTTTTAATACTACATAGTATGATACTCTTTTTTTTCACACTATAAATTTGCTTTCATAAATATTTGGAGATCTTACAACAAGTGAAAGTAATAAGGAAAATTATTCATACTACCGTAATATCCGTAATATAGAAGTTTTTTGTATAGCTTCTTATAATGAACTAATTATTGAGTTCATGTATGTTTGGTGTTAAATAGGAACCAGGAAAGGCAGAGGAAGAGTTTGCGTCGGTGGATATAAGAGAGCTTTTCAAGAACGTGCTGTCAAATCGAGACCCACGTGCTCCATATTTGCCTGGTGAGGTCAAATTTGAAGGGGTCCCACCGCCGCCGCTAGCACCGTGGCTCACTCCCCAAGATATCGACTTTTATGCCGACAAGTTCTCCCACACTGGCTTCACCGGCGGCCTCAACTATTACCGAGCTTTCGACCGGTAATAACAATTATCTACATCATTTTCCTAACATTTATGTTCTCCTAATACTTAACCATTTAGAGAAACAAAATATAAATTTCTTTTAATTTTAAATTATAGAATTCCATTTGAAACCAACTCGGAAAGATTGTAACAGTAACATCTTTAATGATTGAAAGGGAAGTGAATGAATGTTTGAGTTTGAAATGCAGGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATAAAAGTACCAGTAAAGTTCATAGTGGGAGATTTAGATCTGACTTACCATTTTCCAGGAGCTCAAAAGTACATCAATGGCCATGCCTTCAAAAAGGATGTGCCTGGTTTGGAAGAAGTGATTGTTATGAAAGATACTTCTCATTTCATCAACCAAGAAAGGCCTCATGAAATCAACTGCTACGTTCATGATTTCTTCAACAAATTCTGTTGATTCTCTTTCGGTTTTCCTTTTCAGTTTCCATGTTTTATATATGCTAATTTCTGTGGGATTGATTTGTGTCTCTCTTTCCCATTTTCTTTTTCATTTGTATATCAAATGATAAGGGTTTCAATAAGGGAAAATAAATATGCTTCATTTGCTTTTTATGGT

mRNA sequence

ATAAAGTTGATTGTTCCATAACGCCAATGATATCAATTTCGAATTGGCTTAAAATTCCCCTTCTTTCTATTTAAACAAACCCACCACTCAACGAATTCCCAAACACACACAACCAATGGACACCATCCACCACACCACCGTTCAAACCAACGGCATCAAACTCCACGTCGCCTCCGCCGGCCCTGTAACCGGCCCACCCGTGCTCCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCCTGGCGCCACCAGATCGTTTTCCTCTCCTCCGTCGGGTACCGAGTCATCGCCCCGGACCTCCGCGGCTACGGTGACTCGGACGCGCCGCCGTCCGCCGAGTCATACACCACCCTGCACATCGTGGGGGATTTGGTGGGGCTGTTGGATGAGCTGGGGATTGAGAAGGTGTTATTGGTGGGCCATGATTGGGGAGCCTTGATTGCTTGGTACTTTTGCTTGTTTAGGCCGGATAAGATCAAGGCGTCGGTGATTTTGAGCGTCCAGTTTTTTCCTAGAAACCCAACAACGCCATTTATAGAAGGCTTCAAAGCTGTTCTTGGAGACCAATTTTATATGGTCAGATTTCAGGTCGAACCAGGAAAGGCAGAGGAAGAGTTTGCGTCGGTGGATATAAGAGAGCTTTTCAAGAACGTGCTGTCAAATCGAGACCCACGTGCTCCATATTTGCCTGGTGAGGTCAAATTTGAAGGGGTCCCACCGCCGCCGCTAGCACCGTGGCTCACTCCCCAAGATATCGACTTTTATGCCGACAAGTTCTCCCACACTGGCTTCACCGGCGGCCTCAACTATTACCGAGCTTTCGACCGAATTCCATTTGAAACCAACTCGGAAAGATTGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATAAAAGTACCAGTAAAGTTCATAGTGGGAGATTTAGATCTGACTTACCATTTTCCAGGAGCTCAAAAGTACATCAATGGCCATGCCTTCAAAAAGGATGTGCCTGGTTTGGAAGAAGTGATTGTTATGAAAGATACTTCTCATTTCATCAACCAAGAAAGGCCTCATGAAATCAACTGCTACGTTCATGATTTCTTCAACAAATTCTGTTGATTCTCTTTCGGTTTTCCTTTTCAGTTTCCATGTTTTATATATGCTAATTTCTGTGGGATTGATTTGTGTCTCTCTTTCCCATTTTCTTTTTCATTTGTATATCAAATGATAAGGGTTTCAATAAGGGAAAATAAATATGCTTCATTTGCTTTTTATGGT

Coding sequence (CDS)

ATGGACACCATCCACCACACCACCGTTCAAACCAACGGCATCAAACTCCACGTCGCCTCCGCCGGCCCTGTAACCGGCCCACCCGTGCTCCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCCTGGCGCCACCAGATCGTTTTCCTCTCCTCCGTCGGGTACCGAGTCATCGCCCCGGACCTCCGCGGCTACGGTGACTCGGACGCGCCGCCGTCCGCCGAGTCATACACCACCCTGCACATCGTGGGGGATTTGGTGGGGCTGTTGGATGAGCTGGGGATTGAGAAGGTGTTATTGGTGGGCCATGATTGGGGAGCCTTGATTGCTTGGTACTTTTGCTTGTTTAGGCCGGATAAGATCAAGGCGTCGGTGATTTTGAGCGTCCAGTTTTTTCCTAGAAACCCAACAACGCCATTTATAGAAGGCTTCAAAGCTGTTCTTGGAGACCAATTTTATATGGTCAGATTTCAGGTCGAACCAGGAAAGGCAGAGGAAGAGTTTGCGTCGGTGGATATAAGAGAGCTTTTCAAGAACGTGCTGTCAAATCGAGACCCACGTGCTCCATATTTGCCTGGTGAGGTCAAATTTGAAGGGGTCCCACCGCCGCCGCTAGCACCGTGGCTCACTCCCCAAGATATCGACTTTTATGCCGACAAGTTCTCCCACACTGGCTTCACCGGCGGCCTCAACTATTACCGAGCTTTCGACCGAATTCCATTTGAAACCAACTCGGAAAGATTGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATAAAAGTACCAGTAAAGTTCATAGTGGGAGATTTAGATCTGACTTACCATTTTCCAGGAGCTCAAAAGTACATCAATGGCCATGCCTTCAAAAAGGATGTGCCTGGTTTGGAAGAAGTGATTGTTATGAAAGATACTTCTCATTTCATCAACCAAGAAAGGCCTCATGAAATCAACTGCTACGTTCATGATTTCTTCAACAAATTCTGTTGA

Protein sequence

MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVLSNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPFETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQERPHEINCYVHDFFNKFC

Homology

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BLAST of Lsi03G010850 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 7.1e-56
Identity = 120/324 (37.04%), Postives = 166/324 (51.23%), Query Frame = 0


Query: 9   VQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSD 68
           V TNG++L V  AG    P V+L HGFPEL YSWRHQI  L+  GY V+APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  APPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSV 128
            P + E+Y    +  DLVGLLD++G E+ + VGHDWGA++ W   L   D++ A   LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 QFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVLSNRDP--- 188
              PR    P  + F++  G+ F+ + +  EPG A+ E      R + + +   R P   
Sbjct: 129 PALPRAQVPP-TQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 ---RAPYLPGEVKFEGVPPPP--LAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 248
                   PG   F    P P  L  W++ +++D Y  +F+ TGFTGGLN+YR FDR   
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDR--- 248

Query: 249 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 308
                   WE TA   G  I VP  FI G  D    F    +        + + G    +
Sbjct: 249 -------NWETTADLAGKTISVPSLFIAGTADPVLTFTRTDR------AAEVISGPYREV 308

Query: 309 VMKDTSHFINQERPHEINCYVHDF 325
           ++    H++ QERP E+   + +F
Sbjct: 309 LIDGAGHWLQQERPGEVTAALLEF 315

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BLAST of Lsi03G010850 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.3e-46
Identity = 111/315 (35.24%), Postives = 163/315 (51.75%), Query Frame = 0


Query: 13  GIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSDAPPS 72
           G++LH    G  +GP V L HGFPE W+SWR+QI  L+  G+RV+A D++GYG+S APP 
Sbjct: 247 GVRLHFVEMG--SGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPE 306

Query: 73  AESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSVQFFP 132
            E Y+   +  D+V  L++LG+ + + +GHDWG ++ W   LF P++++A   L+  F P
Sbjct: 307 IEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMP 366

Query: 133 RNPTTPFIEGFKA--VLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVLSNRDP----- 192
            NP    +E  KA  V   Q Y   FQ EPG AE E    ++   FKN     D      
Sbjct: 367 SNPNVSPMEIIKANPVFDYQLY---FQ-EPGVAEAELEQ-NLDRTFKNFFRAHDETFLTT 426

Query: 193 -RAPYLPGEVKFEGVP-PPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPFETN 252
            R   L G   F G P  P L+  +T +DI FY  +F  +GF G LN+YR  +R      
Sbjct: 427 NRVRELGG--LFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMER------ 486

Query: 253 SERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMK 312
                W+     +G +I +P   +  + DL  H P   K++        +P L+   + K
Sbjct: 487 ----NWQWGCKGSGRKILIPALMVTAENDLVLH-PKMSKHMENW-----IPHLKRGHI-K 535

Query: 313 DTSHFINQERPHEIN 319
           D  H+   ++P E+N
Sbjct: 547 DCGHWTQIDKPAELN 535

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BLAST of Lsi03G010850 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 102/312 (32.69%), Postives = 157/312 (50.32%), Query Frame = 0


Query: 14  IKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSDAPPSA 73
           ++LH    G  +GP V L HGFPE WYSWR+QI  L+  GYRV+A D++GYG+S APP  
Sbjct: 248 VRLHFVELG--SGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEI 307

Query: 74  ESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSVQFFPR 133
           E Y    +  ++V  LD+LG+ + + +GHDWG ++ WY  LF P++++A   L+  F P 
Sbjct: 308 EEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPA 367

Query: 134 NPTTPFIEGFKA--VLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVLSNRDPRAPYL- 193
           NP    +E  KA  V   Q Y   FQ EPG AE E    ++   FK++    D     + 
Sbjct: 368 NPNMSPLESIKANPVFDYQLY---FQ-EPGVAEAELEQ-NLSRTFKSLFRASDESVLSMH 427

Query: 194 ----PGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPFETNSER 253
                G +       P L+  +T ++I FY  +F  +GF G LN+YR  +R         
Sbjct: 428 KVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMER--------- 487

Query: 254 LTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTS 313
             W+      G +I +P   +  + D     P   +++        +P L+   + +D  
Sbjct: 488 -NWKWACKSLGRKILIPALMVTAEKDFVL-VPQMSQHMEDW-----IPHLKRGHI-EDCG 535

Query: 314 HFINQERPHEIN 319
           H+   ++P E+N
Sbjct: 548 HWTQMDKPTEVN 535

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BLAST of Lsi03G010850 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 172.6 bits (436), Expect = 7.6e-42
Identity = 104/324 (32.10%), Postives = 164/324 (50.62%), Query Frame = 0


Query: 2   DTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDL 61
           D  H       GI+LH    G  +GP + L HGFPE W+SWR+QI  L+  G+RV+A D+
Sbjct: 234 DVSHGYVTVKPGIRLHFVEMG--SGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDM 293

Query: 62  RGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIK 121
           +GYGDS +PP  E Y    +  ++V  LD+LGI + + +GHDW  ++ W   LF P++++
Sbjct: 294 KGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVR 353

Query: 122 ASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVLS 181
           A   L+  F P +P    ++  +++     Y + FQ EPG AE E    ++   FK+   
Sbjct: 354 AVASLNTPFMPPDPDVSPMKVIRSIPVFN-YQLYFQ-EPGVAEAELEK-NMSRTFKSFFR 413

Query: 182 NRDP-------RAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRA 241
             D        +A  + G +       P L+   T ++I+FY  +F  TGF G LN+YR 
Sbjct: 414 ASDETGFIAVHKATEI-GGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYR- 473

Query: 242 FDRIPFETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVP 301
                   N+ER  W+ +    G +I VP   +  + D+    P   K +     +K +P
Sbjct: 474 --------NTER-NWKWSCKGLGRKILVPALMVTAEKDIVLR-PEMSKNM-----EKWIP 533

Query: 302 GLEEVIVMKDTSHFINQERPHEIN 319
            L+   + +D  H+   E+P E+N
Sbjct: 534 FLKRGHI-EDCGHWTQIEKPTEVN 534

[Close]

BLAST of Lsi03G010850 vs. ExPASy Swiss-Prot
Match: P95276 (Epoxide hydrolase B OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1988 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.7e-41
Identity = 116/360 (32.22%), Postives = 157/360 (43.61%), Query Frame = 0


Query: 6   HTTVQTNGIKLHVASAGP--VTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRG 65
           H  +   G ++H  +  P    GP V+LLHGFPE WYSWRHQI  L+  GYRV+A D RG
Sbjct: 5   HRILNCRGTRIHAVADSPPDQQGPLVVLLHGFPESWYSWRHQIPALAGAGYRVVAIDQRG 64

Query: 66  YGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKAS 125
           YG S      ++Y    +VGD+VG+LD  G E+  +VGHDWGA +AW F    PD+    
Sbjct: 65  YGRSSKYRVQKAYRIKELVGDVVGVLDSYGAEQAFVVGHDWGAPVAWTFAWLHPDRCAGV 124

Query: 126 VILSVQFFPRN----PTTPFIEGFK-------AVLGDQFYMVRFQVEPG---KAEEEF-- 185
           V +SV F  R     P +PF E          A  G  +Y   F V+ G   + EE+   
Sbjct: 125 VGISVPFAGRGVIGLPGSPFGERRPSDYHLELAGPGRVWYQDYFAVQDGIITEIEEDLRG 184

Query: 186 -----------------------ASVDIRELFKNVLSNRDPRAPYLPGEVKFEGVPPPPL 245
                                  A VD+  +    +    P        +K   V P  +
Sbjct: 185 WLLGLTYTVSGEGMMAATKAAVDAGVDLESMDPIDVIRAGPLCMAEGARLKDAFVYPETM 244

Query: 246 APWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPFETNSERLTWELTAPWTGAQIKVPV 305
             W T  D+DFY  +F  +GF G L++Y   D            W   A   G  +  P 
Sbjct: 245 PAWFTEADLDFYTGEFERSGFGGPLSFYHNIDN----------DWHDLADQQGKPLTPPA 304

Query: 306 KFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQERPHEINCYVHDF 325
            FI G  D+   + GAQ     H   + +P      ++ D  H+I QE P E N  + DF
Sbjct: 305 LFIGGQYDVGTIW-GAQAIERAH---EVMPNYRGTHMIADVGHWIQQEAPEETNRLLLDF 350

[Close]

BLAST of Lsi03G010850 vs. ExPASy TrEMBL
Match: A0A0A0KV84 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001910 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.7e-169
Identity = 282/329 (85.71%), Postives = 302/329 (91.79%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI+H TVQTNGI LHVA+AGPVTGPPVLLLHGFPELWYSWRHQI+FLSSVGYRVIAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS+++YT LHIVGD+VGLL+ELGI+KVLLVGHDWGALIAWYFCLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KASVILSVQFFPRNP   F+EGFKAVLGDQFYMVRFQ EPGKAE+EFASVDIRE FKNV+
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQ-EPGKAEKEFASVDIREFFKNVM 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDP APYLPGE KFEGVPPP LAPWLTPQDID+YA KFSH+GFTGGLNYYRAFDR   
Sbjct: 181 SNRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTAPWT A+IKVPVKFIVGDLDLTYHFPG Q YING AF+KDVPGLEEVI
Sbjct: 241 -------TWELTAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVI 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           VMKDTSHFINQERP EINC++HDFFNKFC
Sbjct: 301 VMKDTSHFINQERPDEINCHIHDFFNKFC 318

[Close]

BLAST of Lsi03G010850 vs. ExPASy TrEMBL
Match: A0A5A7TNE2 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001190 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.3e-163
Identity = 275/329 (83.59%), Postives = 294/329 (89.36%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 33  METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 92

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 93  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 152

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYMVRFQ EPGKAE+EFASVDIRE FKNV+
Sbjct: 153 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQ-EPGKAEKEFASVDIREFFKNVM 212

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDR   
Sbjct: 213 SNRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDR--- 272

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVI
Sbjct: 273 -------TWELTAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVI 332

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           V+ D SHFINQERP EIN ++HDFF  FC
Sbjct: 333 VIGDASHFINQERPVEINSHIHDFFRNFC 350

[Close]

BLAST of Lsi03G010850 vs. ExPASy TrEMBL
Match: A0A1S3BXU7 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.3e-163
Identity = 275/329 (83.59%), Postives = 294/329 (89.36%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 1   METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYMVRFQ EPGKAE+EFASVDIRE FKNV+
Sbjct: 121 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQ-EPGKAEKEFASVDIREFFKNVM 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDR   
Sbjct: 181 SNRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVI
Sbjct: 241 -------TWELTAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVI 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           V+ D SHFINQERP EIN ++HDFF  FC
Sbjct: 301 VIGDASHFINQERPVEINSHIHDFFRNFC 318

[Close]

BLAST of Lsi03G010850 vs. ExPASy TrEMBL
Match: A0A6J1KD67 (uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.1e-159
Identity = 262/329 (79.64%), Postives = 294/329 (89.36%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ IHHTTV+TNGI LH+A+ G  TGPPVLLLHGFPE WYSWRHQ+++LSS+GYR IAPD
Sbjct: 1   MEAIHHTTVKTNGINLHIAAVG--TGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPSA++YTTLHIVGDLVGLLDELGIEKVLLVG+DWGA+IAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGNDWGAIIAWYLCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KA+VILSVQFFPRNPTTPF+E FK+VLGDQFYMVRFQ +PG+AEEEF +VDI++ F  V+
Sbjct: 121 KAAVILSVQFFPRNPTTPFVEAFKSVLGDQFYMVRFQ-QPGQAEEEFGTVDIKKFFNEVM 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDPRAPYLPGEVKFEG+PPPP A WLTP+DID YAD FS TGFTGGLNYYRAFDR   
Sbjct: 181 SNRDPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADMFSRTGFTGGLNYYRAFDR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTA WT A+IKVPVKFIVGDLDLTYHFPGAQ+YI+G  FK+DVPGLEEV+
Sbjct: 241 -------TWELTAAWTRAEIKVPVKFIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVV 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           VMKDTSHFINQERP+E N ++HDFFNKFC
Sbjct: 301 VMKDTSHFINQERPNETNAHIHDFFNKFC 316

[Close]

BLAST of Lsi03G010850 vs. ExPASy TrEMBL
Match: A0A6J1GAQ3 (uncharacterized protein LOC111452460 OS=Cucurbita moschata OX=3662 GN=LOC111452460 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.9e-157
Identity = 258/329 (78.42%), Postives = 295/329 (89.67%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ I HTTV+TNGI LH+A+ G  TGPPVLLLHGFPE WYSWRHQ+++LSS+GYR IAPD
Sbjct: 1   MEAIQHTTVKTNGINLHIAAVG--TGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPSA++YTTLHIVGDLVGLLDELGIEKVLLVGHDWGA+IAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGHDWGAIIAWYLCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KA+VILSVQFFPRNPTTPF++ FK+VLGDQFYMVRFQ +PG+AEEEFA+VDI++ F +V+
Sbjct: 121 KAAVILSVQFFPRNPTTPFVQAFKSVLGDQFYMVRFQ-QPGQAEEEFATVDIKKFFNDVM 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDPRAPYLPGEVKFEG+PPPP A WLTP+DID YADKFS TGFTGGLNYYRAF+R   
Sbjct: 181 SNRDPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADKFSRTGFTGGLNYYRAFNR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTA WT ++I+VPVK+IVGDLDLTYHFPGAQ+YI+G  FK+DVPGLEEV+
Sbjct: 241 -------TWELTAAWTKSEIRVPVKYIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVV 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           VMKDTSHFINQERP+E N ++HDFF KFC
Sbjct: 301 VMKDTSHFINQERPNETNAHIHDFFKKFC 316

[Close]

BLAST of Lsi03G010850 vs. NCBI nr
Match: XP_038905173.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 631.3 bits (1627), Expect = 4.6e-177
Identity = 297/329 (90.27%), Postives = 307/329 (93.31%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           MDTIHHTTVQTNGIKLHVA+AGPVTGPPVLLLHGFPELWYSWRHQIVFLSS GYRVIAPD
Sbjct: 1   MDTIHHTTVQTNGIKLHVAAAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           L GYGDSDAP S++SYT LHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI
Sbjct: 61  LHGYGDSDAPSSSDSYTALHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KASVILSVQFFPRNP TPFIEGFKAVLGDQFYMVRFQ EPGKAEEEFA+VDIRE FKN L
Sbjct: 121 KASVILSVQFFPRNPKTPFIEGFKAVLGDQFYMVRFQ-EPGKAEEEFATVDIREFFKNTL 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDPRAPYLPGEVKFEGVPPPPLAPWLTP+DIDFYA KFSHTGFTG LNYYRAFDR   
Sbjct: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPEDIDFYAQKFSHTGFTGSLNYYRAFDR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTAPWTGAQI+VPVKFIVGDLDLTYHFPGAQKYI+G AFKKDVPGL+EVI
Sbjct: 241 -------TWELTAPWTGAQIEVPVKFIVGDLDLTYHFPGAQKYIHGDAFKKDVPGLDEVI 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           VMKD SHFINQERPHEINC++HDFFNKFC
Sbjct: 301 VMKDASHFINQERPHEINCHIHDFFNKFC 318

[Close]

BLAST of Lsi03G010850 vs. NCBI nr
Match: XP_004152243.1 (uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical protein Csa_014427 [Cucumis sativus])

HSP 1 Score: 605.1 bits (1559), Expect = 3.5e-169
Identity = 282/329 (85.71%), Postives = 302/329 (91.79%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI+H TVQTNGI LHVA+AGPVTGPPVLLLHGFPELWYSWRHQI+FLSSVGYRVIAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS+++YT LHIVGD+VGLL+ELGI+KVLLVGHDWGALIAWYFCLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KASVILSVQFFPRNP   F+EGFKAVLGDQFYMVRFQ EPGKAE+EFASVDIRE FKNV+
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQ-EPGKAEKEFASVDIREFFKNVM 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDP APYLPGE KFEGVPPP LAPWLTPQDID+YA KFSH+GFTGGLNYYRAFDR   
Sbjct: 181 SNRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTAPWT A+IKVPVKFIVGDLDLTYHFPG Q YING AF+KDVPGLEEVI
Sbjct: 241 -------TWELTAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVI 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           VMKDTSHFINQERP EINC++HDFFNKFC
Sbjct: 301 VMKDTSHFINQERPDEINCHIHDFFNKFC 318

[Close]

BLAST of Lsi03G010850 vs. NCBI nr
Match: KAA0044388.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29516.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 275/329 (83.59%), Postives = 294/329 (89.36%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 33  METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 92

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 93  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 152

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYMVRFQ EPGKAE+EFASVDIRE FKNV+
Sbjct: 153 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQ-EPGKAEKEFASVDIREFFKNVM 212

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDR   
Sbjct: 213 SNRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDR--- 272

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVI
Sbjct: 273 -------TWELTAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVI 332

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           V+ D SHFINQERP EIN ++HDFF  FC
Sbjct: 333 VIGDASHFINQERPVEINSHIHDFFRNFC 350

[Close]

BLAST of Lsi03G010850 vs. NCBI nr
Match: XP_008454324.2 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 275/329 (83.59%), Postives = 294/329 (89.36%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 1   METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYMVRFQ EPGKAE+EFASVDIRE FKNV+
Sbjct: 121 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQ-EPGKAEKEFASVDIREFFKNVM 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDR   
Sbjct: 181 SNRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVI
Sbjct: 241 -------TWELTAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVI 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           V+ D SHFINQERP EIN ++HDFF  FC
Sbjct: 301 VIGDASHFINQERPVEINSHIHDFFRNFC 318

[Close]

BLAST of Lsi03G010850 vs. NCBI nr
Match: XP_022998019.1 (uncharacterized protein LOC111492796 [Cucurbita maxima])

HSP 1 Score: 570.1 bits (1468), Expect = 1.3e-158
Identity = 262/329 (79.64%), Postives = 294/329 (89.36%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ IHHTTV+TNGI LH+A+ G  TGPPVLLLHGFPE WYSWRHQ+++LSS+GYR IAPD
Sbjct: 1   MEAIHHTTVKTNGINLHIAAVG--TGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPSA++YTTLHIVGDLVGLLDELGIEKVLLVG+DWGA+IAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGNDWGAIIAWYLCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
           KA+VILSVQFFPRNPTTPF+E FK+VLGDQFYMVRFQ +PG+AEEEF +VDI++ F  V+
Sbjct: 121 KAAVILSVQFFPRNPTTPFVEAFKSVLGDQFYMVRFQ-QPGQAEEEFGTVDIKKFFNEVM 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIPF 240
           SNRDPRAPYLPGEVKFEG+PPPP A WLTP+DID YAD FS TGFTGGLNYYRAFDR   
Sbjct: 181 SNRDPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADMFSRTGFTGGLNYYRAFDR--- 240

Query: 241 ETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVI 300
                  TWELTA WT A+IKVPVKFIVGDLDLTYHFPGAQ+YI+G  FK+DVPGLEEV+
Sbjct: 241 -------TWELTAAWTRAEIKVPVKFIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVV 300

Query: 301 VMKDTSHFINQERPHEINCYVHDFFNKFC 330
           VMKDTSHFINQERP+E N ++HDFFNKFC
Sbjct: 301 VMKDTSHFINQERPNETNAHIHDFFNKFC 316

[Close]

BLAST of Lsi03G010850 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 3.9e-118
Identity = 192/329 (58.36%), Postives = 246/329 (74.77%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ I HTT+ TNGI +HVAS G  +GP +L +HGFP+LWYSWRHQ+V  +++GYR IAPD
Sbjct: 1   MEKIEHTTISTNGINMHVASIG--SGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS ESYT LHIVGDLVGLLD LG+++V LVGHDWGA++AW+ C+ RPD++
Sbjct: 61  LRGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRV 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNVL 180
            A V  SV F PRNP+   ++ F+A+ GD +Y+ RFQ EPG+ EE+FA VD ++L     
Sbjct: 121 NALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQ-EPGEIEEDFAQVDTKKLITRFF 180

Query: 181 SNRDPRAPYLPGEVKFEGVPPPPLAP-WLTPQDIDFYADKFSHTGFTGGLNYYRAFDRIP 240
           ++R+PR P +P  V F G+P PP  P WLT QD+ FY DKFS  GFTGGLNYYRA +   
Sbjct: 181 TSRNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALN--- 240

Query: 241 FETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEV 300
                  L+WELTAPWTG QIKVPVKFIVGDLD+TY+ PG ++YI+    KK VP L+EV
Sbjct: 241 -------LSWELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEV 300

Query: 301 IVMKDTSHFINQERPHEINCYVHDFFNKF 329
           +VM+   HF++QE+P E+  +++ FF KF
Sbjct: 301 VVMEGVGHFLHQEKPDEVTDHIYGFFKKF 316

[Close]

BLAST of Lsi03G010850 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 2.9e-105
Identity = 180/334 (53.89%), Postives = 224/334 (67.07%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ I H  V  NGI +H+A  GP  GP VLLLHGFP+LWY+WRHQI  LSS+GYR +APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDEL--GIEKVLLVGHDWGALIAWYFCLFRPD 120
           LRGYGDSD+P S   YT L++VGDLV LLD +    EKV LVGHDWGA+I W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 KIKASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKN 180
           KI   V LSV +  RNP    ++GFKAV GD +Y+ RFQ EPGK E E AS D R   +N
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQ-EPGKIEGEIASADPRIFLRN 180

Query: 181 VLSNRDPRAPYLPGEVKFEGVPPP-----PLAPWLTPQDIDFYADKFSHTGFTGGLNYYR 240
           + + R    P LP +  F   P P      L  W + +D+DFY  KF   GFTGGLNYYR
Sbjct: 181 LFTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYR 240

Query: 241 AFDRIPFETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDV 300
           A D          L WELTAPWTGA+I+VPVKF+ GD D+ Y  PG ++YI+G  F  DV
Sbjct: 241 AMD----------LNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADV 300

Query: 301 PGLEEVIVMKDTSHFINQERPHEINCYVHDFFNK 328
           P L+E++V++D  HF+NQE+P E+  +++DFF K
Sbjct: 301 PTLQEIVVIEDAGHFVNQEKPQEVTAHINDFFTK 323

[Close]

BLAST of Lsi03G010850 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 8.0e-95
Identity = 165/335 (49.25%), Postives = 224/335 (66.87%), Query Frame = 0


Query: 1   MDTIHHTTVQTNGIKLHVA---SAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVI 60
           +D + H T++ NGI +HVA    +G    P +L LHGFPELWY+WRHQ+V LSS+GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDAPPSAESYTTLHIVGDLVGLLDEL--GIEKVLLVGHDWGALIAWYFCLF 120
           APDLRGYGD++AP   E YT L++ GD+V L+D +  G + V +VGHDWGA+IAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDKIKASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIREL 180
           RP+K+KA V +SV F PRNP    +   + V GD +Y+ RFQ + G+ E EF  +    +
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQ-KAGEIETEFKKLGTENV 230

Query: 181 FKNVLSNRDPRAPYLPGEVKFEGV--PPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYR 240
            K  L+ + P    LP +  F+        L  WLT +D+D+Y  K+ + GFTG +NYYR
Sbjct: 231 LKEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYR 290

Query: 241 AFDRIPFETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDV 300
             DR           WELTAPWTGA+I+VPVKFI+GD DLTY+FPGA++YING  FK+DV
Sbjct: 291 NIDR----------NWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDV 350

Query: 301 PGLEEVIVMKDTSHFINQERPHEINCYVHDFFNKF 329
           P L+E +V+K   HF+++E P  IN ++H+FF+KF
Sbjct: 351 PLLDETVVLKGLGHFLHEENPDVINQHIHNFFHKF 374

[Close]

BLAST of Lsi03G010850 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-91
Identity = 166/332 (50.00%), Postives = 215/332 (64.76%), Query Frame = 0


Query: 4   IHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRG 63
           + H  V+ NGI +HVA  GP  GP VLLLHGFPELWYSWRHQI  L++ GYR +APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPPSAESYTTLHIVGDLVGLLDELGI---EKVLLVGHDWGALIAWYFCLFRPDKI 123
           YGDSDAP    SYT  +IVGDL+ ++  L     EKV +VGHDWGALIAWY CLFRPD++
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KASVILSV--QFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKN 183
           KA V LSV   F P +P+   ++  +A  GD +Y+ RFQ E G  E E A V    + K 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQ-EFGDVEAEIAEVGTERVMKR 180

Query: 184 VLSNRDPRAPYLPGEVKFEGVPPP--PLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFD 243
           +L+ R P    +P +  F G      PL  WLT +D+ ++  KF   GF+G +NYYR F+
Sbjct: 181 LLTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFN 240

Query: 244 RIPFETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGL 303
           R            EL  PW G++I+VP KF++G+LDL Y+ PG ++YI+G  FK+DVP L
Sbjct: 241 R----------NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLL 300

Query: 304 EEVIVMKDTSHFINQERPHEINCYVHDFFNKF 329
           EE +VM+  +HFINQE+P EI   + DF +KF
Sbjct: 301 EEPVVMEGVAHFINQEKPQEILQIILDFISKF 321

[Close]

BLAST of Lsi03G010850 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.2e-89
Identity = 163/331 (49.24%), Postives = 214/331 (64.65%), Query Frame = 0


Query: 4   IHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRG 63
           + H  V+ NGI +HVA  GP  G  VLLLHGFPELWYSWRHQI  L++ GYR +APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPPSAESYTTLHIVGDLVGLLDELGIE--KVLLVGHDWGALIAWYFCLFRPDKIK 123
           YGDSDAP    S+T  +IVGDLV ++  L  E  KV +VGHDWGALIAWY CLFRPDK+K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ASVILSV--QFFPRNPTTPFIEGFKAVLGDQFYMVRFQVEPGKAEEEFASVDIRELFKNV 183
           A V LSV   F+P +P+   ++  +AV G+ +Y+ RFQ E G  E E A V    + K +
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQ-EVGDIEAEIAEVGTERVMKRL 180

Query: 184 LSNRDPRAPYLPGEVKFEGVPPP--PLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDR 243
           L+ R P    +P +  F G      PL  WLT +D+ ++  KF   GF G +NYYR F+R
Sbjct: 181 LTYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNR 240

Query: 244 IPFETNSERLTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLE 303
                       EL  PW G++I+VP KF++G+LDL Y+ PG ++YI+G  FK+DVP +E
Sbjct: 241 ----------NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIE 300

Query: 304 EVIVMKDTSHFINQERPHEINCYVHDFFNKF 329
           E +VM+  +HF+NQE+P EI   + DF + F
Sbjct: 301 EPVVMEGVAHFLNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:

BLAST of Lsi03G010850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I6YGS0	7.1e-56	37.04	Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX...	[more]
Q6Q2C2	2.3e-46	35.24	Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1	[more]
P34913	1.3e-44	32.69	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]
P34914	7.6e-42	32.10	Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2	[more]
P95276	1.7e-41	32.22	Epoxide hydrolase B OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX...	[more]

BLAST of Lsi03G010850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV84	1.7e-169	85.71	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001...	[more]
A0A5A7TNE2	5.3e-163	83.59	Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BXU7	5.3e-163	83.59	bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE...	[more]
A0A6J1KD67	6.1e-159	79.64	uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796...	[more]
A0A6J1GAQ3	1.9e-157	78.42	uncharacterized protein LOC111452460 OS=Cucurbita moschata OX=3662 GN=LOC1114524...	[more]

BLAST of Lsi03G010850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905173.1	4.6e-177	90.27	epoxide hydrolase A-like [Benincasa hispida]	[more]
XP_004152243.1	3.5e-169	85.71	uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical ...	[more]
KAA0044388.1	1.1e-162	83.59	bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29516.1 bif...	[more]
XP_008454324.2	1.1e-162	83.59	PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo]	[more]
XP_022998019.1	1.3e-158	79.64	uncharacterized protein LOC111492796 [Cucurbita maxima]	[more]

BLAST of Lsi03G010850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02340.1	3.9e-118	58.36	alpha/beta-Hydrolases superfamily protein	[more]
AT3G05600.1	2.9e-105	53.89	alpha/beta-Hydrolases superfamily protein	[more]
AT4G15960.1	8.0e-95	49.25	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26740.1	1.8e-91	50.00	soluble epoxide hydrolase	[more]
AT2G26750.1	2.2e-89	49.24	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 302..324 score: 33.99 coord: 99..112 score: 55.52 coord: 113..126 score: 42.53 coord: 53..68 score: 64.77 coord: 262..278 score: 38.77 coord: 33..51 score: 58.85
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 53..68 score: 53.23 coord: 113..126 score: 16.01 coord: 99..112 score: 33.25
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 27..136 e-value: 4.6E-24 score: 85.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 1..328 e-value: 2.7E-101 score: 341.5
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 3..327
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 1..328
None	No IPR available	PANTHER	PTHR43329:SF58	OS05G0273800 PROTEIN	coord: 1..328

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G010850.1	Lsi03G010850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003824	catalytic activity