Lsi03G010840 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G010840
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionEpoxide hydrolase 2
Locationchr03: 20716563 .. 20717718 (-)
RNA-Seq ExpressionLsi03G010840
SyntenyLsi03G010840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATAATTAAAAAAGAAAAGAAAATAACCCAGAGAGCAATGGAGAAAATAGAGCACACAACAGTTCCCACTAATGGCATCAAAATGCACCTCGCATCGATCGGCTCCGGCCCGGCGGTGCTCTTCCTTCACGGCTTTCCGGAGCTCTGGTACTCCTGGCGCCATCAGCTCCTCTTCCTCGCCTCCAATGGTTTCCGAGCCCTAGCCCCAGATCTCCGCGGCTTCGGCGACACCGACGCGCCCCCGTCTCCTTCCTCCTATACCGCCCACCACATCGTCGGCGATCTCATCGGCCTCCTCGATCATTTCAAAATTGACCAAGTTTTCTTAGTCGGCCACGATTGGGGCGCGATGATTGCCTGGTACTTCTGCTTGTTCAGACCTGACAGAGTTAGGGCTTTAGTGAATTTGAGCGTTCATTATCCTCAACGCCATCCATCCATCAATTTCGTCGATGGATTTAGAGCTTTACTCGGCGATAATTTCTACATCTGTCAATTTCAGGTAAATTAAACCCAACTTTGGTTGAGTCGTTATAGCTTTGGATTTAACCGATTTATTTGAAATTGGGCGTTAGGAACCTGGAGTTGCAGAAGCTGAATTTGGATCTGTTGATACAGCGACAATGTTGAAGAAATTCCTGACAATGAGAGATCCTGAGCCTCCAATAATTACAAAAGAGAAGGGATTCAGTGCGCTGGAAACTCCAGATCCTCTGCCTTGTTGGTTAACAGAGGAAGATATTGATTATTACGCTAGCAAATTCATCAAAACAGGTTTCACCGGAGGATTCAACTACTATCGAGCATTTAATCTGTAAATAACTCATAAACTAACACAAATTAAGTTTTTAACAGATTCAAAGGCATTCCAATGGTATATAATATATGTTTTTATGTTATGGTTGCAGAATATGGGAACTGACGGCGGCATGGAGTGGATCAAAGATTGAAGTTCCAGTGAAATTCATAGTGGGGGATCTTGATTTGGTTTATCATTTTCCAGGTGCGAAAGAGTATATACACGGCGGAGGGTTCAAGAAAAACGTACCGTTACTGGAAGAAGTGGTGGTGATAAAAGGAGCAGCTCATTTCATTAACCAAGAAAAGGCAGATGAAATAAACTACCTCATTTATGACTTCATCGATAAGTTCTGA

mRNA sequence

TTATAATTAAAAAAGAAAAGAAAATAACCCAGAGAGCAATGGAGAAAATAGAGCACACAACAGTTCCCACTAATGGCATCAAAATGCACCTCGCATCGATCGGCTCCGGCCCGGCGGTGCTCTTCCTTCACGGCTTTCCGGAGCTCTGGTACTCCTGGCGCCATCAGCTCCTCTTCCTCGCCTCCAATGGTTTCCGAGCCCTAGCCCCAGATCTCCGCGGCTTCGGCGACACCGACGCGCCCCCGTCTCCTTCCTCCTATACCGCCCACCACATCGTCGGCGATCTCATCGGCCTCCTCGATCATTTCAAAATTGACCAAGTTTTCTTAGTCGGCCACGATTGGGGCGCGATGATTGCCTGGTACTTCTGCTTGTTCAGACCTGACAGAGTTAGGGCTTTAGTGAATTTGAGCGTTCATTATCCTCAACGCCATCCATCCATCAATTTCGTCGATGGATTTAGAGCTTTACTCGGCGATAATTTCTACATCTGTCAATTTCAGGAACCTGGAGTTGCAGAAGCTGAATTTGGATCTGTTGATACAGCGACAATGTTGAAGAAATTCCTGACAATGAGAGATCCTGAGCCTCCAATAATTACAAAAGAGAAGGGATTCAGTGCGCTGGAAACTCCAGATCCTCTGCCTTGTTGGTTAACAGAGGAAGATATTGATTATTACGCTAGCAAATTCATCAAAACAGGTTTCACCGGAGGATTCAACTACTATCGAGCATTTAATCTAATATGGGAACTGACGGCGGCATGGAGTGGATCAAAGATTGAAGTTCCAGTGAAATTCATAGTGGGGGATCTTGATTTGGTTTATCATTTTCCAGGTGCGAAAGAGTATATACACGGCGGAGGGTTCAAGAAAAACGTACCGTTACTGGAAGAAGTGGTGGTGATAAAAGGAGCAGCTCATTTCATTAACCAAGAAAAGGCAGATGAAATAAACTACCTCATTTATGACTTCATCGATAAGTTCTGA

Coding sequence (CDS)

ATGGAGAAAATAGAGCACACAACAGTTCCCACTAATGGCATCAAAATGCACCTCGCATCGATCGGCTCCGGCCCGGCGGTGCTCTTCCTTCACGGCTTTCCGGAGCTCTGGTACTCCTGGCGCCATCAGCTCCTCTTCCTCGCCTCCAATGGTTTCCGAGCCCTAGCCCCAGATCTCCGCGGCTTCGGCGACACCGACGCGCCCCCGTCTCCTTCCTCCTATACCGCCCACCACATCGTCGGCGATCTCATCGGCCTCCTCGATCATTTCAAAATTGACCAAGTTTTCTTAGTCGGCCACGATTGGGGCGCGATGATTGCCTGGTACTTCTGCTTGTTCAGACCTGACAGAGTTAGGGCTTTAGTGAATTTGAGCGTTCATTATCCTCAACGCCATCCATCCATCAATTTCGTCGATGGATTTAGAGCTTTACTCGGCGATAATTTCTACATCTGTCAATTTCAGGAACCTGGAGTTGCAGAAGCTGAATTTGGATCTGTTGATACAGCGACAATGTTGAAGAAATTCCTGACAATGAGAGATCCTGAGCCTCCAATAATTACAAAAGAGAAGGGATTCAGTGCGCTGGAAACTCCAGATCCTCTGCCTTGTTGGTTAACAGAGGAAGATATTGATTATTACGCTAGCAAATTCATCAAAACAGGTTTCACCGGAGGATTCAACTACTATCGAGCATTTAATCTAATATGGGAACTGACGGCGGCATGGAGTGGATCAAAGATTGAAGTTCCAGTGAAATTCATAGTGGGGGATCTTGATTTGGTTTATCATTTTCCAGGTGCGAAAGAGTATATACACGGCGGAGGGTTCAAGAAAAACGTACCGTTACTGGAAGAAGTGGTGGTGATAAAAGGAGCAGCTCATTTCATTAACCAAGAAAAGGCAGATGAAATAAACTACCTCATTTATGACTTCATCGATAAGTTCTGA

Protein sequence

MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRGFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRALVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMRDPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELTAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQEKADEINYLIYDFIDKF
Homology
BLAST of Lsi03G010840 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.1e-56
Identity = 124/318 (38.99%), Postives = 169/318 (53.14%), Query Frame = 0

Query: 9   VPTNGIKMHLASIG--SGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRGFGDTD 68
           V TNG+++ +   G    P V+  HGFPEL YSWRHQ+  LA  G+  LAPD RG+G + 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  APPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRALVNLSV 128
            P +  +Y  H +  DL+GLLD    ++   VGHDWGA++ W   L   DRV A+  LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 HYPQRHPSINFVDGFRALLGDN-FYICQFQEPGVAEAEFGSVDTATMLKKFLTMRDPEPP 188
               R   +     FR+  G+N FYI  FQEPG+A+AE       TM +    +R P   
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 ------IITKEKGF-SALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWE 248
                 +     GF   L  P  LP W+++E++D+Y  +F +TGFTGG N+YR F+  WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 249 LTAAWSGSKIEVPVKFIVGDLDLVYHF---PGAKEYIHGGGFKKNVPLLEEVVVIKGAAH 308
            TA  +G  I VP  FI G  D V  F     A E I G       P  E  V+I GA H
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG-------PYRE--VLIDGAGH 308

Query: 309 FINQEKADEINYLIYDFI 314
           ++ QE+  E+   + +F+
Sbjct: 309 WLQQERPGEVTAALLEFL 316

BLAST of Lsi03G010840 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.6e-47
Identity = 104/307 (33.88%), Postives = 167/307 (54.40%), Query Frame = 0

Query: 13  GIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRGFGDTDAPPSPS 72
           G+++H   +GSGPAV   HGFPE W+SWR+Q+  LA  GFR LA D++G+G++ APP   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRALVNLSVHYPQRH 132
            Y+   +  D++  L+   + Q   +GHDWG ++ W   LF P+RVRA+ +L+  +   +
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMRDPEPPIIT---K 192
           P+++ ++  +A    ++ +  FQEPGVAEAE    +     K F    D E  + T   +
Sbjct: 367 PNVSPMEIIKANPVFDYQL-YFQEPGVAEAEL-EQNLDRTFKNFFRAHD-ETFLTTNRVR 426

Query: 193 EKGFSALETPD--PLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELTAAWSGSK 252
           E G   + TP+   L   +TEEDI +Y  +F K+GF G  N+YR     W+     SG K
Sbjct: 427 ELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRK 486

Query: 253 IEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQEKADEINY 312
           I +P   +  + DLV H   +K        +  +P L+    IK   H+   +K  E+N 
Sbjct: 487 ILIPALMVTAENDLVLHPKMSKH------MENWIPHLKR-GHIKDCGHWTQIDKPAELNR 543

Query: 313 LIYDFID 315
           ++ ++++
Sbjct: 547 ILIEWLE 543

BLAST of Lsi03G010840 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.9e-46
Identity = 103/305 (33.77%), Postives = 161/305 (52.79%), Query Frame = 0

Query: 13  GIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRGFGDTDAPPSPS 72
           GI++H   +GSGPA+   HGFPE W+SWR+Q+  LA  GFR LA D++G+GD+ +PP   
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRALVNLSVHYPQRH 132
            Y    +  +++  L+   I Q   +GHDW  ++ W   LF P+RVRA+ +L+      +
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMRDPEPPIITK--E 192
           P ++ ++  R++   N+ +  FQEPGVAEAE     + T    F T  D     + K  E
Sbjct: 365 PEVSPMEVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKATE 424

Query: 193 KGFSALETP-DPLPCWL-TEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELTAAWSGSKI 252
            G   + TP DP    + TEE+I+YY  +F K+GF G  N+YR     W+ +    G KI
Sbjct: 425 MGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRKI 484

Query: 253 EVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQEKADEINYL 312
            VP   +  + D+V     +K        +  +P L+    I+   H+   EK  E+N +
Sbjct: 485 LVPALMVTAEKDIVLRPEMSK------NMENWIPFLKR-GHIEDCGHWTQIEKPAEVNQI 541

Query: 313 IYDFI 314
           +  ++
Sbjct: 545 LIKWL 541

BLAST of Lsi03G010840 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 8.4e-46
Identity = 98/307 (31.92%), Postives = 163/307 (53.09%), Query Frame = 0

Query: 14  IKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRGFGDTDAPPSPSS 73
           +++H   +GSGPAV   HGFPE WYSWR+Q+  LA  G+R LA D++G+G++ APP    
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRALVNLSVHYPQRHP 133
           Y    +  +++  LD   + Q   +GHDWG M+ WY  LF P+RVRA+ +L+  +   +P
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 SINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMRDPEPPIITKEK-- 193
           +++ ++  +A    ++ +  FQEPGVAEAE     + T    F   R  +  +++  K  
Sbjct: 368 NMSPLESIKANPVFDYQL-YFQEPGVAEAELEQNLSRTFKSLF---RASDESVLSMHKVC 427

Query: 194 --GFSALETPD--PLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELTAAWSGSK 253
             G   + +P+   L   +TEE+I +Y  +F K+GF G  N+YR     W+      G K
Sbjct: 428 EAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRK 487

Query: 254 IEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQEKADEINY 313
           I +P   +  + D V   P   +++        +P L+    I+   H+   +K  E+N 
Sbjct: 488 ILIPALMVTAEKDFVL-VPQMSQHMEDW-----IPHLKR-GHIEDCGHWTQMDKPTEVNQ 543

Query: 314 LIYDFID 315
           ++  ++D
Sbjct: 548 ILIKWLD 543

BLAST of Lsi03G010840 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 8.4e-46
Identity = 105/306 (34.31%), Postives = 160/306 (52.29%), Query Frame = 0

Query: 13  GIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRGFGDTDAPPSPS 72
           GI++H   +GSGPA+   HGFPE W+SWR+Q+  LA  GFR LA D++G+GD+ +PP   
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRALVNLSVHYPQRH 132
            Y    +  +++  LD   I Q   +GHDW  ++ W   LF P+RVRA+ +L+  +    
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMRDPEPPII---TK 192
           P ++ +   R++   N+ +  FQEPGVAEAE    + +   K F    D    I      
Sbjct: 365 PDVSPMKVIRSIPVFNYQL-YFQEPGVAEAEL-EKNMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 EKGFSALETP-DP-LPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELTAAWSGSK 252
           E G   + TP DP L    TEE+I++Y  +F KTGF G  N+YR     W+ +    G K
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 IEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQEKADEINY 312
           I VP   +  + D+V     +K        +K +P L+    I+   H+   EK  E+N 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKR-GHIEDCGHWTQIEKPTEVNQ 541

Query: 313 LIYDFI 314
           ++  ++
Sbjct: 545 ILIKWL 541

BLAST of Lsi03G010840 vs. ExPASy TrEMBL
Match: A0A6J1J9D3 (uncharacterized protein LOC111482483 OS=Cucurbita maxima OX=3661 GN=LOC111482483 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.3e-166
Identity = 274/316 (86.71%), Postives = 294/316 (93.04%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           ME IEHT VPTNGI MH+ASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRA+APDLR
Sbjct: 1   MENIEHTIVPTNGINMHIASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRAIAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTD PPSPSSYTAHHIVGDLIGLLDH  ID+VFLVGHDWGAM+AWYFCLFRPDRVRA
Sbjct: 61  GFGDTDVPPSPSSYTAHHIVGDLIGLLDHLGIDRVFLVGHDWGAMMAWYFCLFRPDRVRA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSVHY  RHPSI FVDGFRA LGD+FY CQFQEPGVAEA+FGSVDTATMLKKFLTMR
Sbjct: 121 LVNLSVHYLHRHPSIKFVDGFRAFLGDDFYFCQFQEPGVAEADFGSVDTATMLKKFLTMR 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           DP PP+I KEKGF ALETPDPLP WLTEED+DY+ASKF KTGFTGGFNYYRAF+L WELT
Sbjct: 181 DPRPPMIPKEKGFRALETPDPLPSWLTEEDVDYFASKFSKTGFTGGFNYYRAFDLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           A WSGS+++VP KFIVGDLDLVYHFPGAKEYIHGG FK++VP LEEVVVI+GAAHFINQE
Sbjct: 241 APWSGSQVKVPAKFIVGDLDLVYHFPGAKEYIHGGRFKEDVPFLEEVVVIEGAAHFINQE 300

Query: 301 KADEINYLIYDFIDKF 317
           +ADEI+ LIY+FI+KF
Sbjct: 301 RADEISSLIYEFINKF 316

BLAST of Lsi03G010840 vs. ExPASy TrEMBL
Match: A0A6J1DR70 (uncharacterized protein LOC111022402 OS=Momordica charantia OX=3673 GN=LOC111022402 PE=4 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.4e-165
Identity = 273/316 (86.39%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           MEKIEH+TV TNGI MH ASIG+GPAVL LHGFPELWYSWRHQLLFLAS G+RA+APDLR
Sbjct: 1   MEKIEHSTVSTNGINMHFASIGAGPAVLLLHGFPELWYSWRHQLLFLASKGYRAIAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTDAPPSPSSY+AHHIVGDL+GLLDH  IDQVFLVGHDWGAM+AWYFCLFRPDRVRA
Sbjct: 61  GFGDTDAPPSPSSYSAHHIVGDLVGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVRA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSVHY  RHPSI FVDGFRA+LGD+FY CQFQEPGVAEA+FGSVDTATM+KKFLTM 
Sbjct: 121 LVNLSVHYLHRHPSIKFVDGFRAVLGDDFYFCQFQEPGVAEADFGSVDTATMMKKFLTMT 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           DP PPII KEKGF +LETPDPLP WLTEEDIDYYASKF +TGFTGGFNYYRAFNL WELT
Sbjct: 181 DPRPPIIPKEKGFRSLETPDPLPDWLTEEDIDYYASKFARTGFTGGFNYYRAFNLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           A WSG++I+VP K IVGDLDLVYHFPGAKEYIHGGGFKK+VPLLEEVVVI+GAAHFINQE
Sbjct: 241 APWSGAQIKVPAKLIVGDLDLVYHFPGAKEYIHGGGFKKDVPLLEEVVVIEGAAHFINQE 300

Query: 301 KADEINYLIYDFIDKF 317
           +ADEIN  IYDFI+KF
Sbjct: 301 RADEINSHIYDFINKF 316

BLAST of Lsi03G010840 vs. ExPASy TrEMBL
Match: A0A0A0KTC4 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001900 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 272/318 (85.53%), Postives = 295/318 (92.77%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           MEKIEHTT+PTNGI MH+ASIGSGPAVLFLHGFP+LWYSWRHQLLFLAS GFRALAPDLR
Sbjct: 1   MEKIEHTTIPTNGINMHVASIGSGPAVLFLHGFPQLWYSWRHQLLFLASKGFRALAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTDAPPSPSSYT HHI+GDLIGLLDHF +D+VFLVGHDWGA+IAWYFCLFRPDRV+A
Sbjct: 61  GFGDTDAPPSPSSYTFHHIIGDLIGLLDHFGLDKVFLVGHDWGAVIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSVHY +RHPSINFVDGFRA  G+NFYICQFQE GVAEA+FGSVDTATM+KKF+ MR
Sbjct: 121 LVNLSVHYLKRHPSINFVDGFRASAGENFYICQFQEAGVAEADFGSVDTATMMKKFMGMR 180

Query: 181 DP-EPPII-TKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWE 240
           DP  PPI  TKEKGFS+LETP+PLPCWLTEED+D++ASKF KTGFTGGFNYYRA NL WE
Sbjct: 181 DPVAPPIYNTKEKGFSSLETPNPLPCWLTEEDVDFFASKFSKTGFTGGFNYYRALNLSWE 240

Query: 241 LTAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFIN 300
           LTAAW+GSKIEVPVKFIVGDLDLVYHFPGAKEYI+GG FKK+VP LEEVVVIK AAHFIN
Sbjct: 241 LTAAWNGSKIEVPVKFIVGDLDLVYHFPGAKEYINGGEFKKDVPFLEEVVVIKDAAHFIN 300

Query: 301 QEKADEINYLIYDFIDKF 317
           QEK  +IN LIY FI+KF
Sbjct: 301 QEKPHQINSLIYHFINKF 318

BLAST of Lsi03G010840 vs. ExPASy TrEMBL
Match: A0A5D3E2H4 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001180 PE=4 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.8e-161
Identity = 267/317 (84.23%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           MEKIEHTT+PTNGI MH+ASIGSGPAVLFLHGFP+LWYSWRHQLLFLAS GFRALAPDLR
Sbjct: 1   MEKIEHTTIPTNGINMHVASIGSGPAVLFLHGFPQLWYSWRHQLLFLASKGFRALAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTDAPPSPSSYT  HI+GDLIGLLDH  +++VFLVGHDWGAMIAWYFCLFRPDRV+A
Sbjct: 61  GFGDTDAPPSPSSYTFLHIIGDLIGLLDHLGLEKVFLVGHDWGAMIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSV+Y +RHPSI+FVDGFRA+ GDNFYICQFQE GVAEA+FG VDTATM+KKF+ MR
Sbjct: 121 LVNLSVYYIKRHPSISFVDGFRAVAGDNFYICQFQEAGVAEADFGRVDTATMMKKFMGMR 180

Query: 181 DPEPPII-TKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWEL 240
           DPE P+I TKEKGFS++ETPDPLPCWLTEEDID++A+KF KTGFTGGFNYYRA NL WEL
Sbjct: 181 DPEAPLIFTKEKGFSSMETPDPLPCWLTEEDIDFFATKFSKTGFTGGFNYYRALNLSWEL 240

Query: 241 TAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQ 300
           TAAW+GSKIEVPVKFIVGDLDLVYHFPGAK+YIHGG FKK+VP LEEVVVIK AAHFI+Q
Sbjct: 241 TAAWNGSKIEVPVKFIVGDLDLVYHFPGAKQYIHGGEFKKDVPFLEEVVVIKDAAHFIHQ 300

Query: 301 EKADEINYLIYDFIDKF 317
           EK  +IN LIY FI+KF
Sbjct: 301 EKPHQINSLIYHFINKF 317

BLAST of Lsi03G010840 vs. ExPASy TrEMBL
Match: A0A1S3BYG0 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494760 PE=4 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.8e-161
Identity = 267/317 (84.23%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           MEKIEHTT+PTNGI MH+ASIGSGPAVLFLHGFP+LWYSWRHQLLFLAS GFRALAPDLR
Sbjct: 1   MEKIEHTTIPTNGINMHVASIGSGPAVLFLHGFPQLWYSWRHQLLFLASKGFRALAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTDAPPSPSSYT  HI+GDLIGLLDH  +++VFLVGHDWGAMIAWYFCLFRPDRV+A
Sbjct: 61  GFGDTDAPPSPSSYTFLHIIGDLIGLLDHLGLEKVFLVGHDWGAMIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSV+Y +RHPSI+FVDGFRA+ GDNFYICQFQE GVAEA+FG VDTATM+KKF+ MR
Sbjct: 121 LVNLSVYYIKRHPSISFVDGFRAVAGDNFYICQFQEAGVAEADFGRVDTATMMKKFMGMR 180

Query: 181 DPEPPII-TKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWEL 240
           DPE P+I TKEKGFS++ETPDPLPCWLTEEDID++A+KF KTGFTGGFNYYRA NL WEL
Sbjct: 181 DPEAPLIFTKEKGFSSMETPDPLPCWLTEEDIDFFATKFSKTGFTGGFNYYRALNLSWEL 240

Query: 241 TAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQ 300
           TAAW+GSKIEVPVKFIVGDLDLVYHFPGAK+YIHGG FKK+VP LEEVVVIK AAHFI+Q
Sbjct: 241 TAAWNGSKIEVPVKFIVGDLDLVYHFPGAKQYIHGGEFKKDVPFLEEVVVIKDAAHFIHQ 300

Query: 301 EKADEINYLIYDFIDKF 317
           EK  +IN LIY FI+KF
Sbjct: 301 EKPHQINSLIYHFINKF 317

BLAST of Lsi03G010840 vs. NCBI nr
Match: XP_038905063.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 619.4 bits (1596), Expect = 1.7e-173
Identity = 287/316 (90.82%), Postives = 302/316 (95.57%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           MEKIEH T+PTNGI MH+ASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR
Sbjct: 1   MEKIEHITIPTNGINMHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTD+PPSPSSYT HH+VGDLIGLLDH  IDQVFLVGHDWGA+IAWYFCLFRPDRV+A
Sbjct: 61  GFGDTDSPPSPSSYTGHHLVGDLIGLLDHLGIDQVFLVGHDWGAIIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSVHY QRHPSINFVDGFRALLGDNFYICQFQEPG AEA+FGSVDTATMLKKFL MR
Sbjct: 121 LVNLSVHYLQRHPSINFVDGFRALLGDNFYICQFQEPGFAEADFGSVDTATMLKKFLAMR 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           DPEPP+ITKEKGFSALETPDPLP WLTEEDIDYYASKF +TGFTGGFNYYRAFNL WELT
Sbjct: 181 DPEPPMITKEKGFSALETPDPLPAWLTEEDIDYYASKFTQTGFTGGFNYYRAFNLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           AAW+G+KI+ PVKFIVG+ DLVYHFPGAKEYIHGGGFKK+VPLLEEVVVIKGAAHFINQE
Sbjct: 241 AAWTGAKIKAPVKFIVGEDDLVYHFPGAKEYIHGGGFKKDVPLLEEVVVIKGAAHFINQE 300

Query: 301 KADEINYLIYDFIDKF 317
           KADEIN LIYDFI+KF
Sbjct: 301 KADEINSLIYDFINKF 316

BLAST of Lsi03G010840 vs. NCBI nr
Match: XP_023528762.1 (uncharacterized protein LOC111791600 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.9 bits (1535), Expect = 2.0e-166
Identity = 275/316 (87.03%), Postives = 294/316 (93.04%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           ME IEH  VPTNGI MH+ASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRA+APDLR
Sbjct: 1   MENIEHKIVPTNGINMHIASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRAIAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTD PPSPSSYTAHHIVGDL+GLLDH  IDQVFLVGHDWGAM+AWYFCLFRPDRVRA
Sbjct: 61  GFGDTDVPPSPSSYTAHHIVGDLVGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVRA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSVHY  RHPSI FVDGFRALLGD+FY CQFQEPGVAEA+FGSVDTATMLKKFLTMR
Sbjct: 121 LVNLSVHYLPRHPSIKFVDGFRALLGDDFYFCQFQEPGVAEADFGSVDTATMLKKFLTMR 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           DP PP+I KEKGF ALETPDPLP WLTEED+DY+ASKF KTGFTGGFNYYRAF+L WELT
Sbjct: 181 DPRPPMIPKEKGFRALETPDPLPSWLTEEDVDYFASKFSKTGFTGGFNYYRAFDLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           A WSGS+I+VP KFIVGDLDLVYHFPGAKEYIHGG FK++VP LEEVVVI+GAAHFINQE
Sbjct: 241 APWSGSQIKVPTKFIVGDLDLVYHFPGAKEYIHGGRFKEDVPFLEEVVVIEGAAHFINQE 300

Query: 301 KADEINYLIYDFIDKF 317
           +ADEI+ LIY+FI+KF
Sbjct: 301 RADEISSLIYEFINKF 316

BLAST of Lsi03G010840 vs. NCBI nr
Match: XP_022984059.1 (uncharacterized protein LOC111482483 [Cucurbita maxima])

HSP 1 Score: 595.5 bits (1534), Expect = 2.7e-166
Identity = 274/316 (86.71%), Postives = 294/316 (93.04%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           ME IEHT VPTNGI MH+ASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRA+APDLR
Sbjct: 1   MENIEHTIVPTNGINMHIASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRAIAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTD PPSPSSYTAHHIVGDLIGLLDH  ID+VFLVGHDWGAM+AWYFCLFRPDRVRA
Sbjct: 61  GFGDTDVPPSPSSYTAHHIVGDLIGLLDHLGIDRVFLVGHDWGAMMAWYFCLFRPDRVRA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSVHY  RHPSI FVDGFRA LGD+FY CQFQEPGVAEA+FGSVDTATMLKKFLTMR
Sbjct: 121 LVNLSVHYLHRHPSIKFVDGFRAFLGDDFYFCQFQEPGVAEADFGSVDTATMLKKFLTMR 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           DP PP+I KEKGF ALETPDPLP WLTEED+DY+ASKF KTGFTGGFNYYRAF+L WELT
Sbjct: 181 DPRPPMIPKEKGFRALETPDPLPSWLTEEDVDYFASKFSKTGFTGGFNYYRAFDLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           A WSGS+++VP KFIVGDLDLVYHFPGAKEYIHGG FK++VP LEEVVVI+GAAHFINQE
Sbjct: 241 APWSGSQVKVPAKFIVGDLDLVYHFPGAKEYIHGGRFKEDVPFLEEVVVIEGAAHFINQE 300

Query: 301 KADEINYLIYDFIDKF 317
           +ADEI+ LIY+FI+KF
Sbjct: 301 RADEISSLIYEFINKF 316

BLAST of Lsi03G010840 vs. NCBI nr
Match: KAG6581277.1 (hypothetical protein SDJN03_21279, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 273/316 (86.39%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           ME IEHT VPTNGI MH+ASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRA+APDLR
Sbjct: 1   MENIEHTIVPTNGINMHIASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRAIAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTD PPSPSSYTAHHIVGDL+GLLDH  ID+VFLVGHDWGAM+AWYFCLFRPDRVRA
Sbjct: 61  GFGDTDVPPSPSSYTAHHIVGDLVGLLDHLGIDRVFLVGHDWGAMMAWYFCLFRPDRVRA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
            VNLSVHY  RHPSI FVDGFRALLGD+FY CQFQEPGVAEA+FGSVDTATMLKKFLTMR
Sbjct: 121 FVNLSVHYLPRHPSIKFVDGFRALLGDDFYFCQFQEPGVAEADFGSVDTATMLKKFLTMR 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           DP PP+I KEKGF ALETPDPLP WLTEED+DY+ASKF KTGFTGGFNYYRAF+L WELT
Sbjct: 181 DPRPPMIPKEKGFRALETPDPLPSWLTEEDVDYFASKFSKTGFTGGFNYYRAFDLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           A WSGS+I+VP KFIVGDLDLVYH PGAKEYIHGG FK++VP LEEVVVI+GAAHFINQE
Sbjct: 241 APWSGSQIKVPTKFIVGDLDLVYHLPGAKEYIHGGRFKEDVPFLEEVVVIQGAAHFINQE 300

Query: 301 KADEINYLIYDFIDKF 317
           +ADEI+ LIY+FI+KF
Sbjct: 301 RADEISSLIYEFINKF 316

BLAST of Lsi03G010840 vs. NCBI nr
Match: XP_022155261.1 (uncharacterized protein LOC111022402 [Momordica charantia])

HSP 1 Score: 592.0 bits (1525), Expect = 3.0e-165
Identity = 273/316 (86.39%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           MEKIEH+TV TNGI MH ASIG+GPAVL LHGFPELWYSWRHQLLFLAS G+RA+APDLR
Sbjct: 1   MEKIEHSTVSTNGINMHFASIGAGPAVLLLHGFPELWYSWRHQLLFLASKGYRAIAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           GFGDTDAPPSPSSY+AHHIVGDL+GLLDH  IDQVFLVGHDWGAM+AWYFCLFRPDRVRA
Sbjct: 61  GFGDTDAPPSPSSYSAHHIVGDLVGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVRA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVNLSVHY  RHPSI FVDGFRA+LGD+FY CQFQEPGVAEA+FGSVDTATM+KKFLTM 
Sbjct: 121 LVNLSVHYLHRHPSIKFVDGFRAVLGDDFYFCQFQEPGVAEADFGSVDTATMMKKFLTMT 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           DP PPII KEKGF +LETPDPLP WLTEEDIDYYASKF +TGFTGGFNYYRAFNL WELT
Sbjct: 181 DPRPPIIPKEKGFRSLETPDPLPDWLTEEDIDYYASKFARTGFTGGFNYYRAFNLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           A WSG++I+VP K IVGDLDLVYHFPGAKEYIHGGGFKK+VPLLEEVVVI+GAAHFINQE
Sbjct: 241 APWSGAQIKVPAKLIVGDLDLVYHFPGAKEYIHGGGFKKDVPLLEEVVVIEGAAHFINQE 300

Query: 301 KADEINYLIYDFIDKF 317
           +ADEIN  IYDFI+KF
Sbjct: 301 RADEINSHIYDFINKF 316

BLAST of Lsi03G010840 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 451.8 bits (1161), Expect = 4.4e-127
Identity = 200/316 (63.29%), Postives = 245/316 (77.53%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLR 60
           MEKIEHTT+ TNGI MH+ASIGSGP +LF+HGFP+LWYSWRHQL+  A+ G+RA+APDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQVFLVGHDWGAMIAWYFCLFRPDRVRA 120
           G+GD+DAPPS  SYT  HIVGDL+GLLD   +D+VFLVGHDWGA++AW+ C+ RPDRV A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFLTMR 180
           LVN SV +  R+PS+  VD FRAL GD++YIC+FQEPG  E +F  VDT  ++ +F T R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 DPEPPIITKEKGFSALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNLIWELT 240
           +P PP I K  GF  L  P  LP WLTE+D+ +Y  KF + GFTGG NYYRA NL WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 AAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAHFINQE 300
           A W+G +I+VPVKFIVGDLD+ Y+ PG KEYIH GG KK+VP L+EVVV++G  HF++QE
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 KADEINYLIYDFIDKF 317
           K DE+   IY F  KF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of Lsi03G010840 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 377.1 bits (967), Expect = 1.4e-104
Identity = 178/325 (54.77%), Postives = 226/325 (69.54%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLASIG--SGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPD 60
           ME I+H  V  NGI MH+A  G   GP VL LHGFP+LWY+WRHQ+  L+S G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGFGDTDAPPSPSSYTAHHIVGDLIGLLDHFKIDQ--VFLVGHDWGAMIAWYFCLFRPD 120
           LRG+GD+D+P S S YT  ++VGDL+ LLD    +Q  VFLVGHDWGA+I W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RVRALVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKF 180
           ++   V LSV Y  R+P +  V GF+A+ GD++YIC+FQEPG  E E  S D    L+  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTMRDPEPPIITKEKGFSALETPDP------LPCWLTEEDIDYYASKFIKTGFTGGFNYY 240
            T R   PPI+ K+  F   E P+P      LP W +++D+D+Y SKF K GFTGG NYY
Sbjct: 181 FTGRTLGPPILPKDNPFG--EKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYY 240

Query: 241 RAFNLIWELTAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVI 300
           RA +L WELTA W+G+KI+VPVKF+ GD D+VY  PG KEYIHGGGF  +VP L+E+VVI
Sbjct: 241 RAMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVI 300

Query: 301 KGAAHFINQEKADEINYLIYDFIDK 316
           + A HF+NQEK  E+   I DF  K
Sbjct: 301 EDAGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Lsi03G010840 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 376.7 bits (966), Expect = 1.8e-104
Identity = 190/322 (59.01%), Postives = 231/322 (71.74%), Query Frame = 0

Query: 4   IEHTTVPTNGIKMHLASIG--SGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRG 63
           +EH  V  NGI +H+A  G   GP VL LHGFPELWYSWRHQ+  LA+ G+RA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  FGDTDAPPSPSSYTAHHIVGDLIGLLDHF---KIDQVFLVGHDWGAMIAWYFCLFRPDRV 123
           +GD+DAP   SSYT  +IVGDLI ++      + ++VF+VGHDWGA+IAWY CLFRPDRV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 RALVNLSVHYPQR--HPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKF 183
           +ALVNLSV +  R   PS+  VD  RA  GD++YIC+FQE G  EAE   V T  ++K+ 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LTMRDPEPPIITKEKGF--SALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFN 243
           LT R P P II K+K F  S  ET  PLP WLTEED+ Y+ SKF + GF+G  NYYR FN
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGET-IPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFN 240

Query: 244 LIWELTAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAA 303
              EL   W GSKI+VP KF++G+LDLVY+ PG KEYIHG  FK++VPLLEE VV++G A
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVA 300

Query: 304 HFINQEKADEINYLIYDFIDKF 317
           HFINQEK  EI  +I DFI KF
Sbjct: 301 HFINQEKPQEILQIILDFISKF 321

BLAST of Lsi03G010840 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 6.0e-100
Identity = 180/321 (56.07%), Postives = 227/321 (70.72%), Query Frame = 0

Query: 4   IEHTTVPTNGIKMHLASIG--SGPAVLFLHGFPELWYSWRHQLLFLASNGFRALAPDLRG 63
           +EH  V  NGI +H+A  G   G  VL LHGFPELWYSWRHQ+  LA+ G+RA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  FGDTDAPPSPSSYTAHHIVGDLIGLLDHF--KIDQVFLVGHDWGAMIAWYFCLFRPDRVR 123
           +GD+DAP   SS+T  +IVGDL+ ++     +  +VF+VGHDWGA+IAWY CLFRPD+V+
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVNLSV--HYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATMLKKFL 183
           ALVNLSV   +    PS+  VD  RA+ G+++Y+C+FQE G  EAE   V T  ++K+ L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TMRDPEPPIITKEKGF--SALETPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRAFNL 243
           T R P P II K+K F  S  ET  PLP WLTEED+ Y+ SKF + GF G  NYYR FN 
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGET-IPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNR 240

Query: 244 IWELTAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKGAAH 303
             EL   W GSKI+VP KF++G+LDLVY+ PG KEYIHG  FK++VPL+EE VV++G AH
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAH 300

Query: 304 FINQEKADEINYLIYDFIDKF 317
           F+NQEK  EI  +I DFI  F
Sbjct: 301 FLNQEKPQEILQIILDFISTF 320

BLAST of Lsi03G010840 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.1e-98
Identity = 166/324 (51.23%), Postives = 224/324 (69.14%), Query Frame = 0

Query: 1   MEKIEHTTVPTNGIKMHLA-----SIGSGPAVLFLHGFPELWYSWRHQLLFLASNGFRAL 60
           ++ +EH T+  NGI MH+A       G  P +LFLHGFPELWY+WRHQ++ L+S G+R +
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGFGDTDAPPSPSSYTAHHIVGDLIGLLDHFK--IDQVFLVGHDWGAMIAWYFCLF 120
           APDLRG+GDT+AP     YT  ++ GD++ L+D        V +VGHDWGAMIAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRVRALVNLSVHYPQRHPSINFVDGFRALLGDNFYICQFQEPGVAEAEFGSVDTATML 180
           RP++V+ALVN+SV +  R+P    V   R + GD++Y+C+FQ+ G  E EF  + T  +L
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KKFLTMRDPEPPIITKEKGFSALE-TPDPLPCWLTEEDIDYYASKFIKTGFTGGFNYYRA 240
           K+FLT + P P  + K+K F   E     LP WLT+ED+DYY +K+   GFTG  NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 FNLIWELTAAWSGSKIEVPVKFIVGDLDLVYHFPGAKEYIHGGGFKKNVPLLEEVVVIKG 300
            +  WELTA W+G+KI VPVKFI+GD DL Y+FPGAKEYI+GGGFK++VPLL+E VV+KG
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AAHFINQEKADEINYLIYDFIDKF 317
             HF+++E  D IN  I++F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS03.1e-5638.99Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C22.6e-4733.88Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P802994.9e-4633.77Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349138.4e-4631.92Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349148.4e-4634.31Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1J9D31.3e-16686.71uncharacterized protein LOC111482483 OS=Cucurbita maxima OX=3661 GN=LOC111482483... [more]
A0A6J1DR701.4e-16586.39uncharacterized protein LOC111022402 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A0A0KTC41.5e-16285.53AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
A0A5D3E2H44.8e-16184.23Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BYG04.8e-16184.23bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494760 PE... [more]
Match NameE-valueIdentityDescription
XP_038905063.11.7e-17390.82epoxide hydrolase A-like [Benincasa hispida][more]
XP_023528762.12.0e-16687.03uncharacterized protein LOC111791600 [Cucurbita pepo subsp. pepo][more]
XP_022984059.12.7e-16686.71uncharacterized protein LOC111482483 [Cucurbita maxima][more]
KAG6581277.11.7e-16586.39hypothetical protein SDJN03_21279, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022155261.13.0e-16586.39uncharacterized protein LOC111022402 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G02340.14.4e-12763.29alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.11.4e-10454.77alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.8e-10459.01soluble epoxide hydrolase [more]
AT2G26750.16.0e-10056.07alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.11.1e-9851.23alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 97..110
score: 33.0
coord: 51..66
score: 44.61
coord: 111..124
score: 30.05
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..133
e-value: 1.1E-23
score: 84.2
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 51..66
score: 53.12
coord: 290..312
score: 37.15
coord: 31..49
score: 56.94
coord: 97..110
score: 52.6
coord: 111..124
score: 52.92
coord: 250..266
score: 41.71
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..316
e-value: 1.5E-107
score: 362.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..315
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..316
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G010840.1Lsi03G010840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity