Lsi03G007080 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G007080
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMitochondrial fission 1 protein
Locationchr03: 9340615 .. 9343710 (-)
RNA-Seq ExpressionLsi03G007080
SyntenyLsi03G007080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACTTACTTTAAATTTGATTCATAATTATATAAAAACCAAATTCAAAACTAGGAAGAATCTTGCCTTGTTTAAGAGCTCCAAATTCAATTTCTTCCTCGTTAATCATCTCTTACATTTCTTTCTCACTAAGATTCGCAGCAGATTTTCCTTGCACAAATTGGAATCCTGATTTCCTGAGCTTCTCATAATCAACGGATTTGATGGAGGCAAAGATTGGCAAATTGTTCGAGTCCGTTTGTTCGTTCTTCGGCGGTGGCGACCAGATCCCTTGGTGTGATCGTGATGTTATCGCCGTGAGTTCTCTTAAATTCTCGCTTTCAACATCACATTTTCTAATTTGTCTCTAAGAAAACGAAAGAAGAACCATGTCGATTAGGTTAAATGACGTATTAGCTCAAATTTGGTGGATTAGGGGAAGTATAAGCATTAGGTTTTTACTTTGATCGATGAATTAGATGAGGATATACGTTTTCTGTGGTTATATTTTCGAACATAACCTTACGAATAAGCTTTGTCGAAGATGAGATAGGATTTTTTTTGTTATTCCATACAAAAATTGGTGTATAATCGATCCAGGACTTTGGCAATGGAAGTATTTTGAACCATGGTAAGATTGCAGATTGCAACATATTATTTGATTTGAACTGCATATTGAAAATTGCAGGGGTGTGAAAGAGAAGTTGCAGAGGCCGATGAAAGTGCCTCAGAAGAACGCAAGAATGAAAGTATCATGAGGTTATCATGGGCACTAGTTCACTCAAGACAATCTGAGGATATTAATCGTGGCATAGCAATGGTTGAAGGTAAAAGTTCTGCAAGGAATCTCTGTGGTATTTTAGAAATATTCTTGAATTCTTGAGACCCCGTTTCATAATCATCTGGTTTTTAGTTTTTGGTTCTTGAAAATTAAGCTTTTAAACGCTACTTCCACCCCTAAGTTTCTTTGTTTTACTATCCACTTTGTACCTAAAAATCAAGTCGAATTGTTTTTGTTTTTTGAATTTGACTAGGAATTCAAGCTTTTCTATAACAAATGTGAAAATCATAATTGAAAAATAAGGAGGAAACAAACATAAATTTCAAAAATAGAAAACTAAATTCAAAATCAAAAACAGGGCTAAGAGTTTATTGAACTAGGAAGAAGGCAAATATTTCCCCCACGTATGTTTTGTTGTCGGCTGCTTGGTTTAAGAAGGCAACCATCCTTTAATTGATGTCTGAATAAAGTTTTGTAGGATAAAAACATTTTAGGTGGGTGTTCAAAGCTTCAAGCTCTTAAAGGCAAATTTGCTTATTTCAAATATAGTTTGTCTTCTTAATTTGTGTCATCAAAAGTTGGAAAAAGATAGGGTAATAGGGCTTAGATGAAGAAATAATATTATTTCCATTATGTGGTATTTAGTTTGTGAACTTAGAAATTAAACGAGAACTTAAACACCTTAATCATCTTGTCTTCGAATTCTATTATAAATTGGCTTTGACCTTAAAATGGGAGAAGGTACTTCCATTTGAAGGCACTTTGTTCTTGCTTGCTCTATCCTGAACATTCTCAAACTGTAGTTAGTAGTGACATAAGTTGAACCATTTTTTCTCAAAGCTTATTATTTGTGTGGTTTAGTTTTTAAAGTTGGATTAGTATGTCATTTGCAGCTTCCTTAACCAATGCTAGGACCCCTTTGCAACAGAGGGAGAAACTTTATTTGCTGGCTGTTGGATATTACAGAAGTGGTGAATATGCAAGGAGTCGGCAGCTTGTAGAACAATGTTTAGAGGTTTGTTCAAGTTGTTTACTTTTACATTTGATAATGTTATATTTCTAAACCTTGCGACATAGTATTCTATGTTGAGAATTCCACATCGGAAAATGTGAAGAGACGTCGCAATGTTTATAAGATACATAGACTACTCCTTCCATTGCCAGTTAGTTTTGAGATGGATCCTATGGGTAAGGGGTAGTCTGTCCAAAAATAATTGGGTATCGATCCAAGAATGGTGAACCCAAAAGAGGTATTATCTTGAGGGGGCATTTTGAAAATCTTACGTTGAAAAAATAGAGACCTCACAATGTTTATAAGATACATGAGTTAAACCCTCTCATTGCGAATAGGTTTTGAGATAGAATCCCATGTTTATCTAATGTTCTGGTAGTTATAAGTTCATTGGTGGCATCACACATAGTTGCACAATGTCTTGCTTCTAGTTGTAATGCTTCAATTTGTAATTTGGTTTGTTAAATCATGCATTTCCTCTCTGAACCTTACTTGCAAGTCACATGTAGCAACCATAAACTACATAATTAACTATCCTATTAATAACAAAAATCATTCCAATGAATATGGTCCAAGACTCATCATACTTTTTCAAGATCTTGATGAATGAATGAGCCAGCCTAGGCACTGGTTTTATTTATTATTATTATTTTATTTTTATTTTTTTTGGTAGATTGCACCTGATTGGAGGCAGGCCTTAGCACTTAAAAAGAGTGTAGAAGATCAAATTGCAAAAGGTACAATGAAATCCAGAACACCTTTTCTTTAACCTTTTTTACTGCATCGAATCATATGCTTAAAGAATTCGTTTCTCTGTATGTTGATCTGCAGATGGAGTCATCGGTATTGGCATTACAGCTACAGCGGTGGGACTTATAGCTGCCGGGATCGCAGCAGCAGCGAGTCGTCGAAATTGAAATATCTATTCATTCACACTAAAATATATGCACAAGTCAAGTGCTTCCCTCAAGAATGGCTGTCTGATGTTTACAGTTTGCCCATGAGAAGAATCTTTAACAAAACCAAAATATTGAAAGCAAATACCTCATATAGTTCTGCATTATAAGCATTTCTAATTTTAACACTAGAAATCTTGTGTATCTTCTTTTGTTCATTTATTTTTCTTTTGTATTAGAAATATTTCTTTGTATGCTTATTTTTCCCCTATGTTACCTTCTATTCTTGTGACTTTCTTAATGTTATAAAACATATGCTTGGTGTTGAAAATATTGGTGAAACATTTTTGTTTGGGAATTGCAAAAAATAAATCGTAAACTAATAAAGTTATTTTCAAATATAGGAAAATGAACCAAACATTTAC

mRNA sequence

AAAAAAACTTACTTTAAATTTGATTCATAATTATATAAAAACCAAATTCAAAACTAGGAAGAATCTTGCCTTGTTTAAGAGCTCCAAATTCAATTTCTTCCTCGTTAATCATCTCTTACATTTCTTTCTCACTAAGATTCGCAGCAGATTTTCCTTGCACAAATTGGAATCCTGATTTCCTGAGCTTCTCATAATCAACGGATTTGATGGAGGCAAAGATTGGCAAATTGTTCGAGTCCGTTTGTTCGTTCTTCGGCGGTGGCGACCAGATCCCTTGGTGTGATCGTGATGTTATCGCCGGGTGTGAAAGAGAAGTTGCAGAGGCCGATGAAAGTGCCTCAGAAGAACGCAAGAATGAAAGTATCATGAGGTTATCATGGGCACTAGTTCACTCAAGACAATCTGAGGATATTAATCGTGGCATAGCAATGGTTGAAGCTTCCTTAACCAATGCTAGGACCCCTTTGCAACAGAGGGAGAAACTTTATTTGCTGGCTGTTGGATATTACAGAAGTGGTGAATATGCAAGGAGTCGGCAGCTTGTAGAACAATGTTTAGAGATTGCACCTGATTGGAGGCAGGCCTTAGCACTTAAAAAGAGTGTAGAAGATCAAATTGCAAAAGATGGAGTCATCGGTATTGGCATTACAGCTACAGCGGTGGGACTTATAGCTGCCGGGATCGCAGCAGCAGCGAGTCGTCGAAATTGAAATATCTATTCATTCACACTAAAATATATGCACAAGTCAAGTGCTTCCCTCAAGAATGGCTGTCTGATGTTTACAGTTTGCCCATGAGAAGAATCTTTAACAAAACCAAAATATTGAAAGCAAATACCTCATATAGTTCTGCATTATAAGCATTTCTAATTTTAACACTAGAAATCTTGTGTATCTTCTTTTGTTCATTTATTTTTCTTTTGTATTAGAAATATTTCTTTGTATGCTTATTTTTCCCCTATGTTACCTTCTATTCTTGTGACTTTCTTAATGTTATAAAACATATGCTTGGTGTTGAAAATATTGGTGAAACATTTTTGTTTGGGAATTGCAAAAAATAAATCGTAAACTAATAAAGTTATTTTCAAATATAGGAAAATGAACCAAACATTTAC

Coding sequence (CDS)

ATGGAGGCAAAGATTGGCAAATTGTTCGAGTCCGTTTGTTCGTTCTTCGGCGGTGGCGACCAGATCCCTTGGTGTGATCGTGATGTTATCGCCGGGTGTGAAAGAGAAGTTGCAGAGGCCGATGAAAGTGCCTCAGAAGAACGCAAGAATGAAAGTATCATGAGGTTATCATGGGCACTAGTTCACTCAAGACAATCTGAGGATATTAATCGTGGCATAGCAATGGTTGAAGCTTCCTTAACCAATGCTAGGACCCCTTTGCAACAGAGGGAGAAACTTTATTTGCTGGCTGTTGGATATTACAGAAGTGGTGAATATGCAAGGAGTCGGCAGCTTGTAGAACAATGTTTAGAGATTGCACCTGATTGGAGGCAGGCCTTAGCACTTAAAAAGAGTGTAGAAGATCAAATTGCAAAAGATGGAGTCATCGGTATTGGCATTACAGCTACAGCGGTGGGACTTATAGCTGCCGGGATCGCAGCAGCAGCGAGTCGTCGAAATTGA

Protein sequence

MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWALVHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIAPDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN
Homology
BLAST of Lsi03G007080 vs. ExPASy Swiss-Prot
Match: Q9M1J1 (Mitochondrial fission 1 protein A OS=Arabidopsis thaliana OX=3702 GN=FIS1A PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.9e-61
Identity = 118/169 (69.82%), Postives = 142/169 (84.02%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           M+AKIG+ F+SV +FF G D+IPWCD DVIAGCEREV EA +S +E+ K E +MRLSWAL
Sbjct: 1   MDAKIGQFFDSVGTFFSGSDKIPWCDGDVIAGCEREVREATDSGTEDLKKECLMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQ+ED+ RGIAM+EASL ++  PL+ REKLYLLAVGYYRSG Y+RSRQLV++C+E+ 
Sbjct: 61  VHSRQTEDVQRGIAMLEASLESSAPPLEDREKLYLLAVGYYRSGNYSRSRQLVDRCIEMQ 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITAT---AVGLIAAGIAAAASRR 167
            DWRQAL LKK++ED+I KDGVIGIGITAT   AVGLIA GI AA SR+
Sbjct: 121 ADWRQALVLKKTIEDKITKDGVIGIGITATAFGAVGLIAGGIVAAMSRK 169

BLAST of Lsi03G007080 vs. ExPASy Swiss-Prot
Match: Q94CK3 (Mitochondrial fission 1 protein B OS=Arabidopsis thaliana OX=3702 GN=FIS1B PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 8.9e-47
Identity = 99/170 (58.24%), Postives = 128/170 (75.29%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGG-----GDQIPWCDRDVIAGCEREVAEADESASEERKNESIMR 60
           M+A IGK+F+SV  FF G      D+ P CD D+I+GCE+E+AEA +   E RK E IMR
Sbjct: 1   MDAAIGKVFDSVSDFFSGAASASADEFPLCDSDIISGCEKELAEAQD---EGRKKECIMR 60

Query: 61  LSWALVHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQ 120
           LSWALVHS+   DI RGIAM+EA + N  + ++ REKLYLLA+GYYRSG+++RSR  +E+
Sbjct: 61  LSWALVHSKMPSDIQRGIAMLEALVVNDTSAMKLREKLYLLALGYYRSGDFSRSRDCIER 120

Query: 121 CLEIAPDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASR 166
           CLE+ P+  QA ALKK++ED+I KDGVIG+GI  TAVG++ AGIAAA  R
Sbjct: 121 CLEVEPESGQAQALKKAIEDRIVKDGVIGVGIAVTAVGVV-AGIAAAILR 166

BLAST of Lsi03G007080 vs. ExPASy Swiss-Prot
Match: Q6CFJ0 (Mitochondrial fission 1 protein OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=FIS1 PE=3 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 7.1e-12
Identity = 40/110 (36.36%), Postives = 68/110 (61.82%), Query Frame = 0

Query: 57  SWALVHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQC 116
           +W L+ SR+ ED   G+ ++     +  TP ++RE LY LA+G Y+ GEY  +R+  +  
Sbjct: 45  AWGLIKSRKVEDQQLGVQILAQVYKD--TPSRRRECLYYLAIGSYKLGEYTDARKYCDLL 104

Query: 117 LEIAPDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRR 167
           L+I PD  Q+  L++ +ED++AK+G+IGI I    + + AA + A  S++
Sbjct: 105 LQIEPDDPQSAKLRQIIEDKLAKEGMIGIAIVGGVIAVGAAVLGAVLSQK 152

BLAST of Lsi03G007080 vs. ExPASy Swiss-Prot
Match: Q9CQ92 (Mitochondrial fission 1 protein OS=Mus musculus OX=10090 GN=Fis1 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.3e-09
Identity = 41/122 (33.61%), Postives = 67/122 (54.92%), Query Frame = 0

Query: 42  ESASEERKNESIMRLSWALVHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYY 101
           E A+      +    +W LV S+ +EDI RGI ++E  L    +  +QR+ ++ LAVG Y
Sbjct: 24  EQAAGSVSKSTQFEYAWCLVRSKYNEDIRRGIVLLEELLPKG-SKEEQRDYVFYLAVGNY 83

Query: 102 RSGEYARSRQLVEQCLEIAPDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAA 161
           R  EY ++ + V   L+  P   QA  L++ ++  + KDG++G+ I    VG +A G+A 
Sbjct: 84  RLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAI----VGGMALGVAG 140

Query: 162 AA 164
            A
Sbjct: 144 LA 140

BLAST of Lsi03G007080 vs. ExPASy Swiss-Prot
Match: Q5AFF7 (Mitochondrial fission 1 protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=FIS1 PE=3 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 7.4e-09
Identity = 39/113 (34.51%), Postives = 66/113 (58.41%), Query Frame = 0

Query: 57  SWALVHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQC 116
           +W L+ S   +    G+ ++     + ++   +RE LY L++G  + G+Y  +++ VE  
Sbjct: 45  AWGLIKSNHHKQQEYGVQILTELYKSEKS--MRREVLYYLSLGSLKIGDYTNAKRYVEAL 104

Query: 117 LEIAPDWRQALALKKSVEDQIAKDGVIGIGIT--ATAVGLIAAGIAAAASRRN 168
           LEI P+ +QA  L K+++D+I  +G+IGIGI   A AVGL   G+  A  R+N
Sbjct: 105 LEIEPENQQARGLLKTIDDKITTEGLIGIGIAGGALAVGL---GLIGALVRKN 152

BLAST of Lsi03G007080 vs. ExPASy TrEMBL
Match: A0A5A7V667 (Mitochondrial fission 1 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold87G00070 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.0e-82
Identity = 161/167 (96.41%), Postives = 165/167 (98.80%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASLT +RTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLTISRTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 168
           PDWRQAL LKK+VEDQIA+DGVIGIGITATAVGLIAAGIAAAASRRN
Sbjct: 121 PDWRQALTLKKTVEDQIARDGVIGIGITATAVGLIAAGIAAAASRRN 167

BLAST of Lsi03G007080 vs. ExPASy TrEMBL
Match: A0A0A0KY38 (Mitochondrial fission 1 protein OS=Cucumis sativus OX=3659 GN=Csa_4G353620 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.0e-82
Identity = 161/167 (96.41%), Postives = 164/167 (98.20%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESVCSFFGGGDQIPWCDRDVI GCEREVAEADESASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVITGCEREVAEADESASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASLT +RTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLTISRTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 168
           PDWRQAL LKK+VEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN
Sbjct: 121 PDWRQALTLKKTVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 167

BLAST of Lsi03G007080 vs. ExPASy TrEMBL
Match: A0A1S3C1F4 (Mitochondrial fission 1 protein OS=Cucumis melo OX=3656 GN=LOC103495805 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.0e-82
Identity = 161/167 (96.41%), Postives = 165/167 (98.80%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASLT +RTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLTISRTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 168
           PDWRQAL LKK+VED+IAKDGVIGIGITATAVGLIAAGIAAAASRRN
Sbjct: 121 PDWRQALTLKKTVEDKIAKDGVIGIGITATAVGLIAAGIAAAASRRN 167

BLAST of Lsi03G007080 vs. ExPASy TrEMBL
Match: A0A6J1HS90 (Mitochondrial fission 1 protein OS=Cucurbita maxima OX=3661 GN=LOC111466114 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.1e-79
Identity = 156/166 (93.98%), Postives = 161/166 (96.99%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESV SFFGGGDQIPWCDRDVIAGCEREVAEA+E ASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVTSFFGGGDQIPWCDRDVIAGCEREVAEANEGASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASL NARTPL +REKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLINARTPLDRREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRR 167
           PDWRQALALKK+VEDQI KDG+IGIGITATAVGLIAAGIAAAASRR
Sbjct: 121 PDWRQALALKKTVEDQIGKDGIIGIGITATAVGLIAAGIAAAASRR 166

BLAST of Lsi03G007080 vs. ExPASy TrEMBL
Match: A0A6J1EB19 (Mitochondrial fission 1 protein OS=Cucurbita moschata OX=3662 GN=LOC111432492 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 4.5e-78
Identity = 153/166 (92.17%), Postives = 159/166 (95.78%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESV SFFGGGDQIPWCDRDVIAGCEREVAEA+E ASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVTSFFGGGDQIPWCDRDVIAGCEREVAEANEGASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASL NARTPL +REKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLINARTPLDRREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRR 167
           PDWRQAL LKK+VEDQI KDG+IGIGITATAVGL+AAGIAAA SRR
Sbjct: 121 PDWRQALTLKKTVEDQIGKDGIIGIGITATAVGLVAAGIAAAVSRR 166

BLAST of Lsi03G007080 vs. NCBI nr
Match: XP_038882975.1 (mitochondrial fission 1 protein A [Benincasa hispida])

HSP 1 Score: 315.8 bits (808), Expect = 2.2e-82
Identity = 161/167 (96.41%), Postives = 166/167 (99.40%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEA+ESASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEANESASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASLTN++TPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLTNSKTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 168
           PDWRQAL LKK+VEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN
Sbjct: 121 PDWRQALTLKKTVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 167

BLAST of Lsi03G007080 vs. NCBI nr
Match: KAA0062754.1 (mitochondrial fission 1 protein A [Cucumis melo var. makuwa] >TYK08828.1 mitochondrial fission 1 protein A [Cucumis melo var. makuwa])

HSP 1 Score: 314.3 bits (804), Expect = 6.3e-82
Identity = 161/167 (96.41%), Postives = 165/167 (98.80%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASLT +RTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLTISRTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 168
           PDWRQAL LKK+VEDQIA+DGVIGIGITATAVGLIAAGIAAAASRRN
Sbjct: 121 PDWRQALTLKKTVEDQIARDGVIGIGITATAVGLIAAGIAAAASRRN 167

BLAST of Lsi03G007080 vs. NCBI nr
Match: XP_008455692.1 (PREDICTED: mitochondrial fission 1 protein A [Cucumis melo])

HSP 1 Score: 313.9 bits (803), Expect = 8.2e-82
Identity = 161/167 (96.41%), Postives = 165/167 (98.80%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASLT +RTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLTISRTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 168
           PDWRQAL LKK+VED+IAKDGVIGIGITATAVGLIAAGIAAAASRRN
Sbjct: 121 PDWRQALTLKKTVEDKIAKDGVIGIGITATAVGLIAAGIAAAASRRN 167

BLAST of Lsi03G007080 vs. NCBI nr
Match: XP_004142650.1 (mitochondrial fission 1 protein A [Cucumis sativus] >KGN54520.1 hypothetical protein Csa_012119 [Cucumis sativus])

HSP 1 Score: 313.9 bits (803), Expect = 8.2e-82
Identity = 161/167 (96.41%), Postives = 164/167 (98.20%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESVCSFFGGGDQIPWCDRDVI GCEREVAEADESASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVITGCEREVAEADESASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASLT +RTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLTISRTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 168
           PDWRQAL LKK+VEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN
Sbjct: 121 PDWRQALTLKKTVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRRN 167

BLAST of Lsi03G007080 vs. NCBI nr
Match: XP_022966468.1 (mitochondrial fission 1 protein A [Cucurbita maxima])

HSP 1 Score: 303.9 bits (777), Expect = 8.5e-79
Identity = 156/166 (93.98%), Postives = 161/166 (96.99%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           MEAKIGKLFESV SFFGGGDQIPWCDRDVIAGCEREVAEA+E ASEERKNESIMRLSWAL
Sbjct: 1   MEAKIGKLFESVTSFFGGGDQIPWCDRDVIAGCEREVAEANEGASEERKNESIMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQSEDINRGIAM+EASL NARTPL +REKLYLLAVGYYRSGEYARSRQLVEQCLEIA
Sbjct: 61  VHSRQSEDINRGIAMLEASLINARTPLDRREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASRR 167
           PDWRQALALKK+VEDQI KDG+IGIGITATAVGLIAAGIAAAASRR
Sbjct: 121 PDWRQALALKKTVEDQIGKDGIIGIGITATAVGLIAAGIAAAASRR 166

BLAST of Lsi03G007080 vs. TAIR 10
Match: AT3G57090.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 3.5e-62
Identity = 118/169 (69.82%), Postives = 142/169 (84.02%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGGGDQIPWCDRDVIAGCEREVAEADESASEERKNESIMRLSWAL 60
           M+AKIG+ F+SV +FF G D+IPWCD DVIAGCEREV EA +S +E+ K E +MRLSWAL
Sbjct: 1   MDAKIGQFFDSVGTFFSGSDKIPWCDGDVIAGCEREVREATDSGTEDLKKECLMRLSWAL 60

Query: 61  VHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQCLEIA 120
           VHSRQ+ED+ RGIAM+EASL ++  PL+ REKLYLLAVGYYRSG Y+RSRQLV++C+E+ 
Sbjct: 61  VHSRQTEDVQRGIAMLEASLESSAPPLEDREKLYLLAVGYYRSGNYSRSRQLVDRCIEMQ 120

Query: 121 PDWRQALALKKSVEDQIAKDGVIGIGITAT---AVGLIAAGIAAAASRR 167
            DWRQAL LKK++ED+I KDGVIGIGITAT   AVGLIA GI AA SR+
Sbjct: 121 ADWRQALVLKKTIEDKITKDGVIGIGITATAFGAVGLIAGGIVAAMSRK 169

BLAST of Lsi03G007080 vs. TAIR 10
Match: AT5G12390.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 6.3e-48
Identity = 99/170 (58.24%), Postives = 128/170 (75.29%), Query Frame = 0

Query: 1   MEAKIGKLFESVCSFFGG-----GDQIPWCDRDVIAGCEREVAEADESASEERKNESIMR 60
           M+A IGK+F+SV  FF G      D+ P CD D+I+GCE+E+AEA +   E RK E IMR
Sbjct: 1   MDAAIGKVFDSVSDFFSGAASASADEFPLCDSDIISGCEKELAEAQD---EGRKKECIMR 60

Query: 61  LSWALVHSRQSEDINRGIAMVEASLTNARTPLQQREKLYLLAVGYYRSGEYARSRQLVEQ 120
           LSWALVHS+   DI RGIAM+EA + N  + ++ REKLYLLA+GYYRSG+++RSR  +E+
Sbjct: 61  LSWALVHSKMPSDIQRGIAMLEALVVNDTSAMKLREKLYLLALGYYRSGDFSRSRDCIER 120

Query: 121 CLEIAPDWRQALALKKSVEDQIAKDGVIGIGITATAVGLIAAGIAAAASR 166
           CLE+ P+  QA ALKK++ED+I KDGVIG+GI  TAVG++ AGIAAA  R
Sbjct: 121 CLEVEPESGQAQALKKAIEDRIVKDGVIGVGIAVTAVGVV-AGIAAAILR 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M1J14.9e-6169.82Mitochondrial fission 1 protein A OS=Arabidopsis thaliana OX=3702 GN=FIS1A PE=1 ... [more]
Q94CK38.9e-4758.24Mitochondrial fission 1 protein B OS=Arabidopsis thaliana OX=3702 GN=FIS1B PE=1 ... [more]
Q6CFJ07.1e-1236.36Mitochondrial fission 1 protein OS=Yarrowia lipolytica (strain CLIB 122 / E 150)... [more]
Q9CQ924.3e-0933.61Mitochondrial fission 1 protein OS=Mus musculus OX=10090 GN=Fis1 PE=1 SV=1[more]
Q5AFF77.4e-0934.51Mitochondrial fission 1 protein OS=Candida albicans (strain SC5314 / ATCC MYA-28... [more]
Match NameE-valueIdentityDescription
A0A5A7V6673.0e-8296.41Mitochondrial fission 1 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0KY384.0e-8296.41Mitochondrial fission 1 protein OS=Cucumis sativus OX=3659 GN=Csa_4G353620 PE=3 ... [more]
A0A1S3C1F44.0e-8296.41Mitochondrial fission 1 protein OS=Cucumis melo OX=3656 GN=LOC103495805 PE=3 SV=... [more]
A0A6J1HS904.1e-7993.98Mitochondrial fission 1 protein OS=Cucurbita maxima OX=3661 GN=LOC111466114 PE=3... [more]
A0A6J1EB194.5e-7892.17Mitochondrial fission 1 protein OS=Cucurbita moschata OX=3662 GN=LOC111432492 PE... [more]
Match NameE-valueIdentityDescription
XP_038882975.12.2e-8296.41mitochondrial fission 1 protein A [Benincasa hispida][more]
KAA0062754.16.3e-8296.41mitochondrial fission 1 protein A [Cucumis melo var. makuwa] >TYK08828.1 mitocho... [more]
XP_008455692.18.2e-8296.41PREDICTED: mitochondrial fission 1 protein A [Cucumis melo][more]
XP_004142650.18.2e-8296.41mitochondrial fission 1 protein A [Cucumis sativus] >KGN54520.1 hypothetical pro... [more]
XP_022966468.18.5e-7993.98mitochondrial fission 1 protein A [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G57090.13.5e-6269.82Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G12390.16.3e-4858.24Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 30..50
NoneNo IPR availablePANTHERPTHR13247:SF13MITOCHONDRIAL FISSION 1 PROTEIN Bcoord: 1..166
IPR016543Mitochondria fission 1 proteinPIRSFPIRSF008835TPR_repeat_11_Fis1coord: 4..167
e-value: 1.1E-44
score: 149.8
IPR016543Mitochondria fission 1 proteinPANTHERPTHR13247TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11coord: 1..166
IPR028061Fis1, C-terminal tetratricopeptide repeatPFAMPF14853Fis1_TPR_Ccoord: 90..142
e-value: 2.6E-22
score: 78.8
IPR028058Fis1, N-terminal tetratricopeptide repeatPFAMPF14852Fis1_TPR_Ncoord: 51..81
e-value: 1.6E-8
score: 34.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 24..166
e-value: 5.6E-44
score: 151.5
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 10..165
IPR033745Mitochondria fission protein Fis1, cytosolic domainCDDcd12212Fis1coord: 23..141
e-value: 4.03008E-38
score: 124.587

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G007080.1Lsi03G007080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0000266 mitochondrial fission
biological_process GO:0016559 peroxisome fission
cellular_component GO:0031307 integral component of mitochondrial outer membrane
cellular_component GO:0005779 integral component of peroxisomal membrane
molecular_function GO:0005515 protein binding