Lsi03G005860 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G005860
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiondisease resistance protein RML1B-like
Locationchr03: 7276190 .. 7281471 (-)
RNA-Seq ExpressionLsi03G005860
SyntenyLsi03G005860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAAAGTCTAGATAACAAATATTATTTTTTTAAAAAAAAATGGAATGGGCACCAAAAAAAAATAAATGATAAAATAAAAGAAACAAAAAAAAAAAAAAAAAAAAAAAAAACAAATAATGAAGACGACCCACCCAACCACCCCAACAATTCTCTTCACCACTACATTCCATGTAGACACATATACCAATAGATTAAGTACTTTTAATTGAACATAACTTTGTCATAGCTAAGACATGATTAATATTTATCAAATGCAAGGATAAAGAATGCACATATAAGCCAATTACAATCAACATTAATGCAAAATTAACTTCATTCTCATTGGAGTATGTAAAAAACCAACCAACACAACATCACTTCTCTTCACCACCACCTCCATTTATACAACCATAACTCAACCCCTGAATACATCTCAACCCTTTACTACCAATTAGAACAACAATTCATATATACCGAATATACCAAGTTATATTGCATGTAAAGTTAGGGACAACTAATCCTCCATCCAACATATTTTCATAATGTTATGATATATTGTCTATTTTGAGCATAATTGATAAAGAAAAATTTGGCTATAAGAAAANTAAAAATTTTAAACGCGTATACGCGGTGGGATCCGATCCAACCCCGTAATTATCGGTCGGACAATTTTTCGGGGTCGGCACTTTGGAAATCCCTTCTACAAGACTCTATCGTGATAATATAAAATCCCCCGGAAGATGTCCTCACGTCCATCCGCGGAGCGGGGTCTGCCGCGTAGACTTCTCTGATTACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTCTTTCCTTTCTTCGTTTTTGCTTCCATTCTTTTTCCCTTTCTTTCTTTTTTTTCTTTTTTAAAATTTTCTTTCCTTTCTCCGTTTTTGCTTCCATTTTTTTTTTTTGCTTCCATCCCCCAAACCCCTCCTCTCTCCAAACCTTCCCAATTAAACAACCCAATAATTTTTTTTAGGACTTTAAAATTATGTAGTCTATCAATAATAATTAATAAACTTCTATCACTGATGATCTATAATATTACTAATAGACTAAGTTTATTATAGTCTATTAATGATAGGCTCCTATCACTAACAAACTACTTCAATCAGCGATAGAAGTTTATGACTTATAAACTCTTATCAACTAATGTATACTAATTTATTAGTAAATACTCAAGATAAAACTAAAAGTCTAAAACTTATAGAATCTATCAGTAGCTATCATTGATAGACTTCACTATTATGACATAATAATATAAATCTATCAATAATAGCCACTGATATACTTATAAATTAGTCACTCAAATAATGTTATCATTAATTACAAAATTCACTGGCTACTCATATTTTTAAATGTTAGAAAATAATTCACTAAATATTTGTGTATCGGTAATATCAATTATCAGTGATATTAGTGATAGAACTTGGATAGATATCAGTCATAGTCATGTATTAGTGATATAGAACTTAGGTAGATATCAATGATAGAAGTCTATTAGTGATAAAAGTCTATTATTGGTAATTATGATAAAAGTTTCTCAATAATAGCACTGATAGCTTTGAGTGTTAATCTTTTGAAATTGATAGTCACTAATGAAAGTCTATCAGAGATAGCGACTAATATTTTCTATCATTGATAGCTTAATCTTTGATGAATTTCTTTCACATTTAATAGCCACTTATAGAGGTCTATCATTGATAACCAACTTAATGAATTTAATTAATAAAGTTGAATCTCGAACTAAAGTGTAAGTGGGATGCTTAGAAGTTATCACTAATAGCATGTTATCATTGATAGAAGCTATCAACAATAGCATGTTATTTGATGGTAAAAAGGAATGATTGAAGTTATCTTTGATAGCATGTTATTAGTGATAGAAGCTATCACCAATAACCACGATATAAGTGATATCAATGATATTAGTGATAGAACTTAGGTGATATCCATATATCGATGATATCAATGATATAAAAGCCGATTCCATATGATGAAACTCTATTACTGATAGCCATTGCTAAAAGTCATTGGTAATAGCCACTGATACAAGCTATCAATGATATTCGTATATCAATGATATGACTTATGTATATATCAATTAAATGAATGATATTAATGATATTGGTGATATGACTTAGATAAATATCAGTGATAATCACTTATAGACCTCACATTGATAGACTTCTATAACTTATAGCTTTAAATGTTAATATTTGAAAGTTGATAGTTTCCTTTCAAAGTTGATAAATACTTATAAAAGTTTATCACTGATAGCCATTAATAGCCTTGAAGTGTTAATATTTCAAGTTTGATTCCAATTGATGAAAGTGTATCAATAATAGTCAATGATATCTGATAGCAAATTGAAAGTTAATATTTCAATTGTTACTATTTCAAGGTTGTATTAATATTTCTCAAATTGAAAGCTATCATTGATAGCAGCTATCAGTGGTAGAAACTGATAGGTGCTATTAGTGATAGCTTTCAATTTGAGAAAATTGGAAGAAGAAGCGCGAAATGGTGTACATGGACTTTTTAGTCATTTACCAATATGACTATCACTGATAGATGCTATTAGTGATATTTGACGCTAATTTTGTATCATATCCTTAATATGTTTTTCCTTGTACTACATCTTTTATTATTTTAGGTCTGATTTTTATTTGTGCAAATCACCTTTTCAATATAAAGTTTAGAAACATTCATGTATTGTATATTCTTACATGATTATAATAATAATTATTTAATGAATTTCGATGGACTAAATTAAGATTTAGTGAAAGTGTAGAGACTAAAATAAAAAATAAAAAATTACATAGATTAAAATTGAACAAAATTTATCAAAATGAACATGAACTTAGCTGAATTGCATATTAAACAAGATTGAAAATATGAGGACTCTAATAAAGTAAAAAATGTTAAAATAGGTCTCTATTCCATCTCCCTCTTTTTCTATTTCTTCTTTTTTCTCTAAAACTTTCTGTGGGTTTCTTCCAAGAACCGTCCTCCTCTGCCTTTTTCTAACTTTGGAAGACGTTTCCAAGTCGTGGTCGTGGTCGTCGTCTTCTTCTACTTTTTCTTTTAATTTTTCTTTAGTTATTCTTTTAGTTAAAATATCATTTTGATGCATATAAGTTTGGAGTTTGTTGGTTGTTTCTGTAAGTATATATTTGATTTGTTTCAGAACTCACGCTTCAGGTTTGAGTTATCTTCAACTTTCCCATTAATTTGTGCTTCTTTCATTTGTCTTTCGTCAGATTTCTCACTATGATTCAAGCAAGTGGATCATCTTCTTCCTCACGTTTTAGTTGGTGTTCTGATGTATTCTTAAGTTTTAGCAGGGAAGATTCTCTTTCAAACTTCACGAGTCATCTTAATATGGCTTTGCGTCAAAAAGGAATCGATGTCTTTATAGATAACGAGCTTTCGAGGGGTGAAGAAATTTCTGCATCTCTTTTGAAAGCTATTGAAGAATCGAAGATCTCAATTGTTATAATGTCTGAAAACTATGCATCTTCGAGTTGGTATTTGAATGAATTGGTGAAAATCATTATGTGTAACAAATTTGGTGAACAAGTTGTTTTACCAGTTTTTTACAAAGTGGATCCATCTCATGTAAGGAAACAAAGTGGAACATTTGGAGAAGAATTTGCCAAACTTGAAGTTAGATTCTTCAACAAGATGCAAGAATGGAGGGAGGCTCTAACTACTATTTCCAATATGTCAGGAAGGGTTCTACAAAAAGAGTATGTTTTTCCATATCCCTAATACACTTTTGCTCTTTACATTCTATTGTTACTATATATATATATTTTTCATTTGAAGAGAAGAGCATGTAGTTGAGGAGAAAAAGAAAAAAAACAAGATATTTAGGTCCAGTATGATAATAATTTCGTATGCATGACCTTACTTAAACAATATCTGTTGTTGTATAATTGTATCCTTGAGTTTTAAAAGAATTTTGTTTTGTGCTTTTCATTTTTTAAATTTATATATGTTTTTGATCTTCTAATTTTTCAGTTATGGTTTTCAACTTTGTTAAAAGAAACACTTAAATTCCTAATAGAATTCTAAAAACAAAAACATGTTTTTGAAAATAGTTTTCATAACAAGGCTCCATCTTTTAAAATAATAGGAGAAAGTTGGCTAACAAAGAAGGAAGTAGTAAGTAGTGTTTATATGCTTAATTTGCAAAAACTTAAAATTAAATACTAAATGGTTATCAAATGGGCCTTAGATTTTTTTTTTCTTTTCAAATGGGACTTTTTCCATTATATTATTTGTTATGTCCTGTCTCCACCCTTCTGACAGATATTACTTGGTTGGATTTGGATTTAGTTTGAGTTTATTATGTTGCTTTGATTTTCTGGCTTCTAATTTTAGGAATTATTGTGGGAAAAATGTGTGTTTCATAACACTTTTTAGTGTATGCTTTTTTTTTTCTTGACATGTGGCAGTGATGAGGCTAATTTGATTCAAATAATTGTCCAAGACGTCTGGAAGAAATTAAATCGTGCAACGATGCAGTTAGATGTAGCTGAATATCCAGTTGGAATTGACAGACAACTTATGAATTTACTCCCACATGTTATGTCTGATGGAATTAGTATGGTTGGATTATATGGAATTGGAGGTGTGGATAAGACAACTTTGGCCAAAGCTTTATACAATATAATTGCTGATGACTTTGAAGGTTGCTGCTTTCTGGCAGATATTAGAGAAGAGTCAAATCAATGTGGGGGCCTTGTTAGACTCCAGAAGGATCTACTTAGAGAAATTTTAATGGATGATTCTATCAAAGTTAGCAATCATCATAAAGGAATTAGCATCATAAGGCATCGACTATGCTCAAAAAAGATTCTTTTGATTCTTGATAATGTAGATACTCATGAGCAATTAGAGGCATTGCCAGGAGGACATGATTGGTTTGGAGCTGGAAGTAAGGTCATTGCTACAACAAGAAACAAACAATTACTTGCTAGTCATGGATTTGATATATTGCAGAGTGTTGATGAATTGAATGATGATGAAGCACTTGAGCTTTTTAGTTGGCATACTTTTAAGAGAAGTTATCCATCAAGTGATTATTTTGACCTTTCAAAACGTGCGGTAGATTATTGTAAAGGTCTTCCCTTGACTCTTCGAGTTTTAGGTAAATTCCTTTGTCCTATTGATGATCAATCCCAGTTTGAATCTATATTGGATGGATATGAGAATTCCTTTGTACAACGGCATCCAAAATCCTCTTCAAATAAGTTATGA

mRNA sequence

ATGTTTAAAAACTCACGCTTCAGGGAAGATTCTCTTTCAAACTTCACGAGTCATCTTAATATGGCTTTGCGTCAAAAAGGAATCGATGTCTTTATAGATAACGAGCTTTCGAGGGGTGAAGAAATTTCTGCATCTCTTTTGAAAGCTATTGAAGAATCGAAGATCTCAATTGTTATAATGTCTGAAAACTATGCATCTTCGAGTTGGTATTTGAATGAATTGGTGAAAATCATTATGTGTAACAAATTTGGTGAACAAGTTGTTTTACCAGTTTTTTACAAAGTGGATCCATCTCATGTAAGGAAACAAAGTGGAACATTTGGAGAAGAATTTGCCAAACTTGAAGTTAGATTCTTCAACAAGATGCAAGAATGGAGGGAGGCTCTAACTACTATTTCCAATATGTCAGGAAGGGTTCTACAAAAAGATGATGAGGCTAATTTGATTCAAATAATTGTCCAAGACGTCTGGAAGAAATTAAATCGTGCAACGATGCAGTTAGATGTAGCTGAATATCCAGTTGGAATTGACAGACAACTTATGAATTTACTCCCACATGTTATGTCTGATGGAATTAGTATGGTTGGATTATATGGAATTGGAGGTGTGGATAAGACAACTTTGGCCAAAGCTTTATACAATATAATTGCTGATGACTTTGAAGGTTGCTGCTTTCTGGCAGATATTAGAGAAGAGTCAAATCAATGTGGGGGCCTTGTTAGACTCCAGAAGGATCTACTTAGAGAAATTTTAATGGATGATTCTATCAAAGTTAGCAATCATCATAAAGGAATTAGCATCATAAGGCATCGACTATGCTCAAAAAAGATTCTTTTGATTCTTGATAATGTAGATACTCATGAGCAATTAGAGGCATTGCCAGGAGGACATGATTGGTTTGGAGCTGGAAGTAAGGTCATTGCTACAACAAGAAACAAACAATTACTTGCTAGTCATGGATTTGATATATTGCAGAGTGTTGATGAATTGAATGATGATGAAGCACTTGAGCTTTTTAGTTGGCATACTTTTAAGAGAAGTTATCCATCAAGTGATTATTTTGACCTTTCAAAACGTGCGGTAGATTATTGTAAAGGTCTTCCCTTGACTCTTCGAGTTTTAGGTAAATTCCTTTGTCCTATTGATGATCAATCCCAGTTTGAATCTATATTGGATGGATATGAGAATTCCTTTGTACAACGGCATCCAAAATCCTCTTCAAATAAGTTATGA

Coding sequence (CDS)

ATGTTTAAAAACTCACGCTTCAGGGAAGATTCTCTTTCAAACTTCACGAGTCATCTTAATATGGCTTTGCGTCAAAAAGGAATCGATGTCTTTATAGATAACGAGCTTTCGAGGGGTGAAGAAATTTCTGCATCTCTTTTGAAAGCTATTGAAGAATCGAAGATCTCAATTGTTATAATGTCTGAAAACTATGCATCTTCGAGTTGGTATTTGAATGAATTGGTGAAAATCATTATGTGTAACAAATTTGGTGAACAAGTTGTTTTACCAGTTTTTTACAAAGTGGATCCATCTCATGTAAGGAAACAAAGTGGAACATTTGGAGAAGAATTTGCCAAACTTGAAGTTAGATTCTTCAACAAGATGCAAGAATGGAGGGAGGCTCTAACTACTATTTCCAATATGTCAGGAAGGGTTCTACAAAAAGATGATGAGGCTAATTTGATTCAAATAATTGTCCAAGACGTCTGGAAGAAATTAAATCGTGCAACGATGCAGTTAGATGTAGCTGAATATCCAGTTGGAATTGACAGACAACTTATGAATTTACTCCCACATGTTATGTCTGATGGAATTAGTATGGTTGGATTATATGGAATTGGAGGTGTGGATAAGACAACTTTGGCCAAAGCTTTATACAATATAATTGCTGATGACTTTGAAGGTTGCTGCTTTCTGGCAGATATTAGAGAAGAGTCAAATCAATGTGGGGGCCTTGTTAGACTCCAGAAGGATCTACTTAGAGAAATTTTAATGGATGATTCTATCAAAGTTAGCAATCATCATAAAGGAATTAGCATCATAAGGCATCGACTATGCTCAAAAAAGATTCTTTTGATTCTTGATAATGTAGATACTCATGAGCAATTAGAGGCATTGCCAGGAGGACATGATTGGTTTGGAGCTGGAAGTAAGGTCATTGCTACAACAAGAAACAAACAATTACTTGCTAGTCATGGATTTGATATATTGCAGAGTGTTGATGAATTGAATGATGATGAAGCACTTGAGCTTTTTAGTTGGCATACTTTTAAGAGAAGTTATCCATCAAGTGATTATTTTGACCTTTCAAAACGTGCGGTAGATTATTGTAAAGGTCTTCCCTTGACTCTTCGAGTTTTAGGTAAATTCCTTTGTCCTATTGATGATCAATCCCAGTTTGAATCTATATTGGATGGATATGAGAATTCCTTTGTACAACGGCATCCAAAATCCTCTTCAAATAAGTTATGA

Protein sequence

MFKNSRFREDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSSWYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREALTTISNMSGRVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLLREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGLPLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSSSNKL
Homology
BLAST of Lsi03G005860 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.5e-84
Identity = 178/375 (47.47%), Postives = 240/375 (64.00%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+  NFT HL  AL ++GI  F D+ L RGE I+  LLKAIEES+ S+++ SENYA S 
Sbjct: 33  EDTRYNFTDHLYSALGRRGIRTFRDDRLRRGEAIAPELLKAIEESRSSVIVFSENYAHSR 92

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W L+ELVKI+ C K     V P+FY VDPSHVRKQ G+FGE FA  E  + +K+  WR A
Sbjct: 93  WCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYEENWKDKIPRWRTA 152

Query: 129 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQL--MNLLP 188
           LT  +N+SG  +L    E+N I+ I   ++++L     +LDV    VGID  +  M L  
Sbjct: 153 LTEAANLSGWHLLDDRYESNQIKEITNSIFRQLK--CKRLDVGANLVGIDSHVKEMILRL 212

Query: 189 HVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKD 248
           H+ S  + MVG+YG+GG+ KTT+AK +YN ++ +FE   FL +IRE SN    L  LQ  
Sbjct: 213 HLESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNP-QVLFHLQNQ 272

Query: 249 LLREILM-DDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGS 308
           LL +IL  + S  +S+     S+I+  L S+++ ++LD+VD   QLE L G  +W G GS
Sbjct: 273 LLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLGEGS 332

Query: 309 KVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYC 368
           +VI TTRNK +LA    D L  V+ LN +EA ELFS + FK++ P SDY +L+ R V YC
Sbjct: 333 RVIITTRNKHVLAVQEVDDLYEVEGLNFEEACELFSLYAFKQNLPKSDYRNLTCRVVGYC 392

Query: 369 KGLPLTLRVLGKFLC 380
           +GLPL L+VLG  LC
Sbjct: 393 QGLPLALKVLGSLLC 404

BLAST of Lsi03G005860 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.1e-81
Identity = 172/376 (45.74%), Postives = 237/376 (63.03%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+  NFT HL  AL ++GI  F D++L RGE I+  LLKAIEES+ S+++ SENYA S 
Sbjct: 36  EDTRFNFTDHLYSALGRRGIRTFRDDKLRRGEAIAPELLKAIEESRSSVIVFSENYARSR 95

Query: 69  WYLNELVKIIMCNKFGE---QVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEW 128
           W L+ELVKI+ C+K  +     V P+FY VDPSHVRKQ G+FGE FA       +K+  W
Sbjct: 96  WCLDELVKIMECHKDKKDPGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYGENLKDKIPRW 155

Query: 129 REALTTISNMSGRVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLL- 188
           R ALT  +N+SG  LQ   E+N I+ I   ++++L     +LD     VGID  +  ++ 
Sbjct: 156 RTALTEAANLSGWPLQDGYESNQIKEITDSIFRRLK--CKRLDAGANLVGIDSHVKEMIW 215

Query: 189 -PHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQ 248
             H+ S  + MVG+YG+GG+ KTT+AK +YN ++ +FE   FL +IRE+ N   G+  LQ
Sbjct: 216 RLHMESSDVRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIREKFN-TQGVSPLQ 275

Query: 249 KDLLREILM-DDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGA 308
             LL +IL  + S  +++   G S+I+  L SK + ++LD+VD   QLE L    +W G 
Sbjct: 276 NQLLDDILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGE 335

Query: 309 GSKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVD 368
           GS+VI TTRNK +L     D L  V  LN +EA ELFS + F+++ P SDY +LS R V 
Sbjct: 336 GSRVIITTRNKHVLDVQKVDDLYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVG 395

Query: 369 YCKGLPLTLRVLGKFL 379
           YC+GLPL L+VLG  L
Sbjct: 396 YCQGLPLALKVLGCLL 408

BLAST of Lsi03G005860 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.1e-77
Identity = 162/380 (42.63%), Postives = 240/380 (63.16%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDN-ELSRGEEISASLLKAIEESKISIVIMSENYASS 68
           ED+   F  HL  AL +KGI  F+D+ EL RG+ IS+ L+KAI ES+ ++V+ S+NYASS
Sbjct: 21  EDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVVFSKNYASS 80

Query: 69  SWYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFF---NKMQE 128
           +W L ELVKI+  ++  E +V+PVFY VDPS VRKQ+G +   F K E       +K+  
Sbjct: 81  TWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEANLVDDRDKVLR 140

Query: 129 WREALTTISNMSGRVLQ---KDDEANLIQIIVQDVWKKLNRATMQLDVAEYP-VGIDRQL 188
           WREALT ++N+SG  L+     DE+  IQ I++D++ K       + +     VGI+ Q+
Sbjct: 141 WREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKF---CFSISITNRDLVGIESQI 200

Query: 189 MNLLPHVMSD--GISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGG 248
             L   +  D  G+ +VG++G+GGV KTT A+AL+N    +FE  CFL D++E   Q   
Sbjct: 201 KKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNFESACFLEDVKEYL-QHHT 260

Query: 249 LVRLQKDLLREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHD 308
           L+ LQK LL ++L  + +  ++  +   I++ RLCSKK+L++LD+V+ ++QL+ L G  D
Sbjct: 261 LLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHNDQLDKLVGAED 320

Query: 309 WFGAGSKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSK 368
           WFG+GS+++ TTR+ +LL +H       +  L  DEA+ELF+ H FKRS P  ++ +L  
Sbjct: 321 WFGSGSRIVITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLN 380

Query: 369 RAVDYCKGLPLTLRVLGKFL 379
             VDY  GLPL L+VLG  L
Sbjct: 381 LVVDYTGGLPLALKVLGSLL 396

BLAST of Lsi03G005860 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.0e-67
Identity = 154/384 (40.10%), Postives = 225/384 (58.59%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNE-LSRGEEISASLLKAIEESKISIVIMSENYASS 68
           ED+   FTSHL   L  KGI  F D++ L  G  I   L KAIEES+ +IV+ SENYA+S
Sbjct: 21  EDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAIVVFSENYATS 80

Query: 69  SWYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRF---FNKMQE 128
            W LNELVKI+ C    +Q V+P+FY VDPSHVR Q  +F + F + E ++      +Q 
Sbjct: 81  RWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHETKYKDDVEGIQR 140

Query: 129 WREALTTISNMSGRVLQKD-DEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNL 188
           WR AL   +N+ G    +D  +A+ I+ IV  +  KL    + L   +  VGID  L  +
Sbjct: 141 WRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKL--CKISLSYLQNIVGIDTHLEKI 200

Query: 189 --LPHVMSDGISMVGLYGIGGVDKTTLAKALYNII------ADDFEGCCFLADIREESNQ 248
             L  +  +G+ ++G++G+GGV KTT+A+A+++ +      +  F+G CFL DI+E    
Sbjct: 201 ESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKENKR- 260

Query: 249 CGGLVRLQKDLLREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQ-LEALP 308
             G+  LQ  LL E+L + +   +N   G   +  RL SKK+L++LD++D  +  LE L 
Sbjct: 261 --GMHSLQNALLSELLREKA-NYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLA 320

Query: 309 GGHDWFGAGSKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYF 368
           G  DWFG GS++I TTR+K L+  +  DI+  V  L D E+++LF  H F +  P+ ++ 
Sbjct: 321 GDLDWFGNGSRIIITTRDKHLIEKN--DIIYEVTALPDHESIQLFKQHAFGKEVPNENFE 380

Query: 369 DLSKRAVDYCKGLPLTLRVLGKFL 379
            LS   V+Y KGLPL L+V G  L
Sbjct: 381 KLSLEVVNYAKGLPLALKVWGSLL 396

BLAST of Lsi03G005860 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 6.5e-67
Identity = 152/384 (39.58%), Postives = 226/384 (58.85%), Query Frame = 0

Query: 10  DSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSSW 69
           D+ +NFT HL  ALR +GID FID+ L RG+ ++A L   IE+SKI+I++ S NYA+S+W
Sbjct: 21  DTRNNFTGHLQKALRLRGIDSFIDDRLRRGDNLTA-LFDRIEKSKIAIIVFSTNYANSAW 80

Query: 70  YLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRF----FNKMQEW 129
            L ELVKI+ C    +Q+V+P+FYKVD S V KQ  +F   F   E+ F      ++  W
Sbjct: 81  CLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKLPELTFPGVTPEEISSW 140

Query: 130 REALTTISNMSGRVLQK--DDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMN- 189
           + AL + SN+ G V+++    EA L+  I  D +KKLN   +     E  VGI+ +L N 
Sbjct: 141 KAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN--DLAPSGNEGLVGIESRLKNL 200

Query: 190 --LLPHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLV 249
             LL     D + ++G+ G+ G+ KTTLA  LY  +   F+G CFL +IRE S +  GL 
Sbjct: 201 EKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSCFLTNIRENSGR-SGLE 260

Query: 250 RLQKDLLREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWF 309
            L + L   +L D  +++            RL SK++L++LD+V+  +Q+  L G   W+
Sbjct: 261 SLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVNDEKQIRYLMGHCKWY 320

Query: 310 GAGSKVIATTRNKQLLAS-HGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKR 369
             GS++I TTR+ +L+ +  G   +  + +LND EAL+LFS + F  S+P  ++  L+  
Sbjct: 321 QGGSRIIITTRDSKLIETIKGRKYV--LPKLNDREALKLFSLNAFSNSFPLKEFEGLTNM 380

Query: 370 AVDYCKGLPLTLRVLGKFLCPIDD 384
            +DY KG PL L+VLG  LC  DD
Sbjct: 381 VLDYAKGHPLALKVLGSDLCERDD 398

BLAST of Lsi03G005860 vs. ExPASy TrEMBL
Match: D9I8I5 (Putative TIR-NBS-LRR-AAA+ATPase OS=Cucumis sativus OX=3659 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 5.1e-163
Identity = 296/399 (74.19%), Postives = 341/399 (85.46%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEI ASLL+AIE SKISIV++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEICASLLEAIEGSKISIVVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V KQSG FGEEFAKLEVRFFNKMQ W+EA
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGKQSGRFGEEFAKLEVRFFNKMQAWKEA 145

Query: 129 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHV 188
           L T+S+MSG  VLQ+DDEANLIQ IVQ+VWKKL+RATMQLDVA+YPVGID Q+ NLLPHV
Sbjct: 146 LITVSHMSGWPVLQRDDEANLIQNIVQEVWKKLDRATMQLDVAKYPVGIDIQVRNLLPHV 205

Query: 189 MSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLL 248
           MS+GI+M GLYG+GG+ KTT+AKALYN IAD+FEGCCFL++IRE SNQ GGLV+ QK+LL
Sbjct: 206 MSNGITMFGLYGVGGMGKTTIAKALYNKIADEFEGCCFLSNIREASNQYGGLVQFQKELL 265

Query: 249 REILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVI 308
            EILMDDSIKVSN  +GI+IIR+RL SKKILLILD+VDT EQL+AL GGHDWFG GSKVI
Sbjct: 266 CEILMDDSIKVSNLPRGITIIRNRLYSKKILLILDDVDTREQLQALAGGHDWFGHGSKVI 325

Query: 309 ATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGL 368
           ATTRNKQLL +HGFD +Q+V  L+ DEALELFSWH F+ S+P + Y +LSKRAVDYCKGL
Sbjct: 326 ATTRNKQLLVTHGFDKMQNVGGLDYDEALELFSWHCFRNSHPLNVYLELSKRAVDYCKGL 385

Query: 369 PLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           PL L VLG FL  I D S F+ ILD YE  ++ +  + S
Sbjct: 386 PLALEVLGSFLHSIGDPSNFKRILDEYEKHYLDKDIQDS 424

BLAST of Lsi03G005860 vs. ExPASy TrEMBL
Match: A0A0A0LGB6 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G021540 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 5.1e-163
Identity = 296/399 (74.19%), Postives = 341/399 (85.46%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEI ASLL+AIE SKISIV++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEICASLLEAIEGSKISIVVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V KQSG FGEEFAKLEVRFFNKMQ W+EA
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGKQSGRFGEEFAKLEVRFFNKMQAWKEA 145

Query: 129 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHV 188
           L T+S+MSG  VLQ+DDEANLIQ IVQ+VWKKL+RATMQLDVA+YPVGID Q+ NLLPHV
Sbjct: 146 LITVSHMSGWPVLQRDDEANLIQNIVQEVWKKLDRATMQLDVAKYPVGIDIQVSNLLPHV 205

Query: 189 MSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLL 248
           MS+GI+M GLYG+GG+ KTT+AKALYN IAD+FEGCCFL++IRE SNQ GGLV+ QK+LL
Sbjct: 206 MSNGITMFGLYGVGGMGKTTIAKALYNKIADEFEGCCFLSNIREASNQYGGLVQFQKELL 265

Query: 249 REILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVI 308
            EILMDDSIKVSN  +GI+IIR+RL SKKILLILD+VDT EQL+AL GGHDWFG GSKVI
Sbjct: 266 CEILMDDSIKVSNLPRGITIIRNRLYSKKILLILDDVDTREQLQALAGGHDWFGHGSKVI 325

Query: 309 ATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGL 368
           ATTRNKQLL +HGFD +Q+V  L+ DEALELFSWH F+ S+P + Y +LSKRAVDYCKGL
Sbjct: 326 ATTRNKQLLVTHGFDKMQNVGGLDYDEALELFSWHCFRNSHPLNVYLELSKRAVDYCKGL 385

Query: 369 PLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           PL L VLG FL  I D S F+ ILD YE  ++ +  + S
Sbjct: 386 PLALEVLGSFLHSIGDPSNFKRILDEYEKHYLDKDIQDS 424

BLAST of Lsi03G005860 vs. ExPASy TrEMBL
Match: E7CHD0 (Putative TIR-NBS-LRR-AAA+ATPase OS=Cucumis sativus var. hardwickii OX=319220 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.5e-162
Identity = 295/399 (73.93%), Postives = 340/399 (85.21%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEI ASLL+AIE SKISIV++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEICASLLEAIEGSKISIVVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V KQSG FGEEFAKLEVRFFNKMQ W+EA
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGKQSGRFGEEFAKLEVRFFNKMQAWKEA 145

Query: 129 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHV 188
           L T+S+MSG  VLQ+DDEANLIQ IVQ+VWKKL+RATMQLDVA+YPVGID Q+ NLLPHV
Sbjct: 146 LITVSHMSGWPVLQRDDEANLIQNIVQEVWKKLDRATMQLDVAKYPVGIDIQVRNLLPHV 205

Query: 189 MSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLL 248
           MS+GI+M GLYG+GG+ KTT+AKALYN IAD+FEGCCFL++IRE SNQ GGLV+ QK+LL
Sbjct: 206 MSNGITMFGLYGVGGMGKTTIAKALYNKIADEFEGCCFLSNIREASNQYGGLVQFQKELL 265

Query: 249 REILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVI 308
            EILMDDSIKVSN  +GI+IIR+RL SKKILLILD+VD  EQL+AL GGHDWFG GSKVI
Sbjct: 266 CEILMDDSIKVSNLPRGITIIRNRLYSKKILLILDDVDKREQLQALAGGHDWFGHGSKVI 325

Query: 309 ATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGL 368
           ATTRNKQLL +HGFD +Q+V  L+ DEALELFSWH F+ S+P + Y +LSKRAVDYCKGL
Sbjct: 326 ATTRNKQLLVTHGFDKMQNVGGLDYDEALELFSWHCFRNSHPLNVYLELSKRAVDYCKGL 385

Query: 369 PLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           PL L VLG FL  I D S F+ ILD YE  ++ +  + S
Sbjct: 386 PLALEVLGSFLNSIGDPSNFKRILDEYEKHYLDKDIQDS 424

BLAST of Lsi03G005860 vs. ExPASy TrEMBL
Match: Q84KB3 (MRGH63 OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 9.5e-162
Identity = 294/400 (73.50%), Postives = 343/400 (85.75%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEIS+SLL+AIEESK+SI+++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEISSSLLEAIEESKVSIIVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRF-FNKMQEWRE 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V  QSG FGEEFAKLEVRF  +KM+ W+E
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGNQSGRFGEEFAKLEVRFSSDKMEAWKE 145

Query: 129 ALTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPH 188
           AL T+S+MSG  VLQ+DDEANLIQ IVQ+VWK+L+RATMQLDVA+YPVGID Q+ NLLPH
Sbjct: 146 ALITVSHMSGWPVLQRDDEANLIQNIVQEVWKELDRATMQLDVAKYPVGIDIQVRNLLPH 205

Query: 189 VMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDL 248
           VMS+G +MVGLYGIGG+ KTTLAKALYN IADDFEGCCFL +IRE SNQ GGLV+LQ++L
Sbjct: 206 VMSNGTTMVGLYGIGGMGKTTLAKALYNKIADDFEGCCFLPNIREASNQYGGLVQLQREL 265

Query: 249 LREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKV 308
           LREIL+DDSIKVSN  +G++IIR+RL SKKILLILD+VDT EQL+AL GGHDWFG GSKV
Sbjct: 266 LREILVDDSIKVSNLPRGVTIIRNRLYSKKILLILDDVDTREQLQALVGGHDWFGHGSKV 325

Query: 309 IATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKG 368
           IATTRNKQLL +HGFD +QSV  L+ DEALELFSWH F+ S+P +DY +LSKRAVDYCKG
Sbjct: 326 IATTRNKQLLVTHGFDKMQSVVGLDYDEALELFSWHCFRNSHPLNDYLELSKRAVDYCKG 385

Query: 369 LPLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           LPL L VLG FL  IDD   F+ ILD YE  ++ +  + S
Sbjct: 386 LPLALEVLGSFLHSIDDPFNFKRILDEYEKYYLDKEIQDS 425

BLAST of Lsi03G005860 vs. ExPASy TrEMBL
Match: A0A1S3C0Y9 (TMV resistance protein N-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495240 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 9.5e-162
Identity = 294/400 (73.50%), Postives = 343/400 (85.75%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEIS+SLL+AIEESK+SI+++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEISSSLLEAIEESKVSIIVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRF-FNKMQEWRE 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V  QSG FGEEFAKLEVRF  +KM+ W+E
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGNQSGRFGEEFAKLEVRFSSDKMEAWKE 145

Query: 129 ALTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPH 188
           AL T+S+MSG  VLQ+DDEANLIQ IVQ+VWK+L+RATMQLDVA+YPVGID Q+ NLLPH
Sbjct: 146 ALITVSHMSGWPVLQRDDEANLIQNIVQEVWKELDRATMQLDVAKYPVGIDIQVRNLLPH 205

Query: 189 VMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDL 248
           VMS+G +MVGLYGIGG+ KTTLAKALYN IADDFEGCCFL +IRE SNQ GGLV+LQ++L
Sbjct: 206 VMSNGTTMVGLYGIGGMGKTTLAKALYNKIADDFEGCCFLPNIREASNQYGGLVQLQREL 265

Query: 249 LREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKV 308
           LREIL+DDSIKVSN  +G++IIR+RL SKKILLILD+VDT EQL+AL GGHDWFG GSKV
Sbjct: 266 LREILVDDSIKVSNLPRGVTIIRNRLYSKKILLILDDVDTREQLQALVGGHDWFGHGSKV 325

Query: 309 IATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKG 368
           IATTRNKQLL +HGFD +QSV  L+ DEALELFSWH F+ S+P +DY +LSKRAVDYCKG
Sbjct: 326 IATTRNKQLLVTHGFDKMQSVVGLDYDEALELFSWHCFRNSHPLNDYLELSKRAVDYCKG 385

Query: 369 LPLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           LPL L VLG FL  IDD   F+ ILD YE  ++ +  + S
Sbjct: 386 LPLALEVLGSFLHSIDDPFNFKRILDEYEKYYLDKEIQDS 425

BLAST of Lsi03G005860 vs. NCBI nr
Match: XP_038889763.1 (disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889764.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889765.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889766.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889768.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889769.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889770.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889771.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889772.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_038889773.1 disease resistance protein RPV1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 609.4 bits (1570), Expect = 2.3e-170
Identity = 308/393 (78.37%), Postives = 346/393 (88.04%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNMALRQ+GI+VFIDN+LSRGEEIS SLL+AIEESKISIVI+SENYASSS
Sbjct: 25  EDTRSNFTSHLNMALRQRGINVFIDNKLSRGEEISTSLLEAIEESKISIVIISENYASSS 84

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNKFG QVVLPVFYKVDPS VRKQSG FGEEFAKLEVRFFNKMQ WREA
Sbjct: 85  WCLNELVKIIMCNKFGGQVVLPVFYKVDPSQVRKQSGRFGEEFAKLEVRFFNKMQTWREA 144

Query: 129 LTTISNMSGRVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHVM 188
           LTTIS+MSG VL+KDDEA+LIQ IVQ+VWKKLNR TMQL VA+YPVG+D Q+M+LL  VM
Sbjct: 145 LTTISHMSGWVLEKDDEASLIQKIVQEVWKKLNRTTMQLHVAKYPVGVDIQIMSLLSRVM 204

Query: 189 SDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLLR 248
            DGI+MVGLYGIGG+ KTTLAKALYN IAD+FEG CFLA+IRE SNQ GGLV+LQ+ LL 
Sbjct: 205 FDGITMVGLYGIGGMGKTTLAKALYNRIADNFEGWCFLANIREASNQYGGLVQLQEKLLC 264

Query: 249 EILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVIA 308
           E+LMDD IKVSN H+GI+IIR RLCSKKIL++LD++DT EQL  L GGHDWFGAGSKVI 
Sbjct: 265 EVLMDDLIKVSNIHRGINIIRDRLCSKKILVVLDDIDTIEQLRVLAGGHDWFGAGSKVIV 324

Query: 309 TTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGLP 368
           TTRNK LLA HGFDI++SV+ LN+DEALELFSWH FK S P+SDY +LSKRAV YCKGLP
Sbjct: 325 TTRNKHLLAIHGFDIVKSVNGLNNDEALELFSWHAFKNSQPTSDYLELSKRAVHYCKGLP 384

Query: 369 LTLRVLGKFLCPIDDQSQFESILDGYENSFVQR 402
           L L VLG FL  I DQS+F+ ILD YENS++ +
Sbjct: 385 LALEVLGSFLYSI-DQSKFDRILDEYENSYLDK 416

BLAST of Lsi03G005860 vs. NCBI nr
Match: XP_038889774.1 (disease resistance protein RPV1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 609.4 bits (1570), Expect = 2.3e-170
Identity = 308/393 (78.37%), Postives = 346/393 (88.04%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNMALRQ+GI+VFIDN+LSRGEEIS SLL+AIEESKISIVI+SENYASSS
Sbjct: 25  EDTRSNFTSHLNMALRQRGINVFIDNKLSRGEEISTSLLEAIEESKISIVIISENYASSS 84

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNKFG QVVLPVFYKVDPS VRKQSG FGEEFAKLEVRFFNKMQ WREA
Sbjct: 85  WCLNELVKIIMCNKFGGQVVLPVFYKVDPSQVRKQSGRFGEEFAKLEVRFFNKMQTWREA 144

Query: 129 LTTISNMSGRVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHVM 188
           LTTIS+MSG VL+KDDEA+LIQ IVQ+VWKKLNR TMQL VA+YPVG+D Q+M+LL  VM
Sbjct: 145 LTTISHMSGWVLEKDDEASLIQKIVQEVWKKLNRTTMQLHVAKYPVGVDIQIMSLLSRVM 204

Query: 189 SDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLLR 248
            DGI+MVGLYGIGG+ KTTLAKALYN IAD+FEG CFLA+IRE SNQ GGLV+LQ+ LL 
Sbjct: 205 FDGITMVGLYGIGGMGKTTLAKALYNRIADNFEGWCFLANIREASNQYGGLVQLQEKLLC 264

Query: 249 EILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVIA 308
           E+LMDD IKVSN H+GI+IIR RLCSKKIL++LD++DT EQL  L GGHDWFGAGSKVI 
Sbjct: 265 EVLMDDLIKVSNIHRGINIIRDRLCSKKILVVLDDIDTIEQLRVLAGGHDWFGAGSKVIV 324

Query: 309 TTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGLP 368
           TTRNK LLA HGFDI++SV+ LN+DEALELFSWH FK S P+SDY +LSKRAV YCKGLP
Sbjct: 325 TTRNKHLLAIHGFDIVKSVNGLNNDEALELFSWHAFKNSQPTSDYLELSKRAVHYCKGLP 384

Query: 369 LTLRVLGKFLCPIDDQSQFESILDGYENSFVQR 402
           L L VLG FL  I DQS+F+ ILD YENS++ +
Sbjct: 385 LALEVLGSFLYSI-DQSKFDRILDEYENSYLDK 416

BLAST of Lsi03G005860 vs. NCBI nr
Match: ADI99935.1 (TIR-NBS-LRR class resistance protein [Cucumis sativus] >ADU33174.1 putative TIR-NBS-LRR-AAA+ATPase [Cucumis sativus])

HSP 1 Score: 583.9 bits (1504), Expect = 1.0e-162
Identity = 296/399 (74.19%), Postives = 341/399 (85.46%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEI ASLL+AIE SKISIV++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEICASLLEAIEGSKISIVVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V KQSG FGEEFAKLEVRFFNKMQ W+EA
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGKQSGRFGEEFAKLEVRFFNKMQAWKEA 145

Query: 129 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHV 188
           L T+S+MSG  VLQ+DDEANLIQ IVQ+VWKKL+RATMQLDVA+YPVGID Q+ NLLPHV
Sbjct: 146 LITVSHMSGWPVLQRDDEANLIQNIVQEVWKKLDRATMQLDVAKYPVGIDIQVRNLLPHV 205

Query: 189 MSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLL 248
           MS+GI+M GLYG+GG+ KTT+AKALYN IAD+FEGCCFL++IRE SNQ GGLV+ QK+LL
Sbjct: 206 MSNGITMFGLYGVGGMGKTTIAKALYNKIADEFEGCCFLSNIREASNQYGGLVQFQKELL 265

Query: 249 REILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVI 308
            EILMDDSIKVSN  +GI+IIR+RL SKKILLILD+VDT EQL+AL GGHDWFG GSKVI
Sbjct: 266 CEILMDDSIKVSNLPRGITIIRNRLYSKKILLILDDVDTREQLQALAGGHDWFGHGSKVI 325

Query: 309 ATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGL 368
           ATTRNKQLL +HGFD +Q+V  L+ DEALELFSWH F+ S+P + Y +LSKRAVDYCKGL
Sbjct: 326 ATTRNKQLLVTHGFDKMQNVGGLDYDEALELFSWHCFRNSHPLNVYLELSKRAVDYCKGL 385

Query: 369 PLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           PL L VLG FL  I D S F+ ILD YE  ++ +  + S
Sbjct: 386 PLALEVLGSFLHSIGDPSNFKRILDEYEKHYLDKDIQDS 424

BLAST of Lsi03G005860 vs. NCBI nr
Match: XP_011650107.2 (TMV resistance protein N [Cucumis sativus] >KGN60913.2 hypothetical protein Csa_023436 [Cucumis sativus])

HSP 1 Score: 583.9 bits (1504), Expect = 1.0e-162
Identity = 296/399 (74.19%), Postives = 341/399 (85.46%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEI ASLL+AIE SKISIV++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEICASLLEAIEGSKISIVVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V KQSG FGEEFAKLEVRFFNKMQ W+EA
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGKQSGRFGEEFAKLEVRFFNKMQAWKEA 145

Query: 129 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHV 188
           L T+S+MSG  VLQ+DDEANLIQ IVQ+VWKKL+RATMQLDVA+YPVGID Q+ NLLPHV
Sbjct: 146 LITVSHMSGWPVLQRDDEANLIQNIVQEVWKKLDRATMQLDVAKYPVGIDIQVRNLLPHV 205

Query: 189 MSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLL 248
           MS+GI+M GLYG+GG+ KTT+AKALYN IAD+FEGCCFL++IRE SNQ GGLV+ QK+LL
Sbjct: 206 MSNGITMFGLYGVGGMGKTTIAKALYNKIADEFEGCCFLSNIREASNQYGGLVQFQKELL 265

Query: 249 REILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVI 308
            EILMDDSIKVSN  +GI+IIR+RL SKKILLILD+VDT EQL+AL GGHDWFG GSKVI
Sbjct: 266 CEILMDDSIKVSNLPRGITIIRNRLYSKKILLILDDVDTREQLQALAGGHDWFGHGSKVI 325

Query: 309 ATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGL 368
           ATTRNKQLL +HGFD +Q+V  L+ DEALELFSWH F+ S+P + Y +LSKRAVDYCKGL
Sbjct: 326 ATTRNKQLLVTHGFDKMQNVGGLDYDEALELFSWHCFRNSHPLNVYLELSKRAVDYCKGL 385

Query: 369 PLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           PL L VLG FL  I D S F+ ILD YE  ++ +  + S
Sbjct: 386 PLALEVLGSFLHSIGDPSNFKRILDEYEKHYLDKDIQDS 424

BLAST of Lsi03G005860 vs. NCBI nr
Match: ADU33175.1 (putative TIR-NBS-LRR-AAA+ATPase [Cucumis sativus var. hardwickii])

HSP 1 Score: 581.6 bits (1498), Expect = 5.2e-162
Identity = 295/399 (73.93%), Postives = 340/399 (85.21%), Query Frame = 0

Query: 9   EDSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSS 68
           ED+ SNFTSHLNM LRQ+GI+VFID +LSRGEEI ASLL+AIE SKISIV++SE+YASSS
Sbjct: 26  EDTRSNFTSHLNMTLRQRGINVFIDKKLSRGEEICASLLEAIEGSKISIVVISESYASSS 85

Query: 69  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFFNKMQEWREA 128
           W LNELVKIIMCNK   QVVLP+FYKVDPS V KQSG FGEEFAKLEVRFFNKMQ W+EA
Sbjct: 86  WCLNELVKIIMCNKLRGQVVLPIFYKVDPSEVGKQSGRFGEEFAKLEVRFFNKMQAWKEA 145

Query: 129 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNLLPHV 188
           L T+S+MSG  VLQ+DDEANLIQ IVQ+VWKKL+RATMQLDVA+YPVGID Q+ NLLPHV
Sbjct: 146 LITVSHMSGWPVLQRDDEANLIQNIVQEVWKKLDRATMQLDVAKYPVGIDIQVRNLLPHV 205

Query: 189 MSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQKDLL 248
           MS+GI+M GLYG+GG+ KTT+AKALYN IAD+FEGCCFL++IRE SNQ GGLV+ QK+LL
Sbjct: 206 MSNGITMFGLYGVGGMGKTTIAKALYNKIADEFEGCCFLSNIREASNQYGGLVQFQKELL 265

Query: 249 REILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAGSKVI 308
            EILMDDSIKVSN  +GI+IIR+RL SKKILLILD+VD  EQL+AL GGHDWFG GSKVI
Sbjct: 266 CEILMDDSIKVSNLPRGITIIRNRLYSKKILLILDDVDKREQLQALAGGHDWFGHGSKVI 325

Query: 309 ATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDYCKGL 368
           ATTRNKQLL +HGFD +Q+V  L+ DEALELFSWH F+ S+P + Y +LSKRAVDYCKGL
Sbjct: 326 ATTRNKQLLVTHGFDKMQNVGGLDYDEALELFSWHCFRNSHPLNVYLELSKRAVDYCKGL 385

Query: 369 PLTLRVLGKFLCPIDDQSQFESILDGYENSFVQRHPKSS 407
           PL L VLG FL  I D S F+ ILD YE  ++ +  + S
Sbjct: 386 PLALEVLGSFLNSIGDPSNFKRILDEYEKHYLDKDIQDS 424

BLAST of Lsi03G005860 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 270.0 bits (689), Expect = 3.1e-72
Identity = 158/375 (42.13%), Postives = 229/375 (61.07%), Query Frame = 0

Query: 10  DSLSNFTSHLNMALRQKGIDVFIDN-ELSRGEEISASLLKAIEESKISIVIMSENYASSS 69
           D   NF SHL  +LR+ GI  F+D+ EL RGE IS  LL AIE SKI IV+++++YASS+
Sbjct: 24  DVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYASSA 83

Query: 70  WYLNELVKIIMCNKFG-EQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRF-FNKMQEWR 129
           W L+ELV I+  +K     +V P+F  VDPS +R Q G++ + F+K +     NK+++WR
Sbjct: 84  WCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPLNKLKDWR 143

Query: 130 EALTTISNMSGRVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNL--L 189
           EALT ++N+SG  ++  +EA  I  I +++ K+L      L V  Y VG+  +L ++  L
Sbjct: 144 EALTKVANISGWDIKNRNEAECIADITREILKRL--PCQYLHVPSYAVGLRSRLQHISSL 203

Query: 190 PHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQK 249
             + SDG+ ++ +YG+GG+ KTTLAK  +N  +  FEG  FL + RE S +  G   LQ 
Sbjct: 204 LSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQH 263

Query: 250 DLLREILMDDSIKVSNHHKGIS-IIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAG 309
            LL +IL  + I+     KG+   ++ R  SK++LL++D+VD   QL +     D FG G
Sbjct: 264 QLLSDILRRNDIE----FKGLDHAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHG 323

Query: 310 SKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDY 369
           S++I TTRN  LL     +   S  EL+ DE+LELFSWH F+ S P  ++   S+  V Y
Sbjct: 324 SRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTY 383

Query: 370 CKGLPLTLRVLGKFL 379
           C GLPL + VLG FL
Sbjct: 384 CAGLPLAVEVLGAFL 392

BLAST of Lsi03G005860 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 270.0 bits (689), Expect = 3.1e-72
Identity = 158/375 (42.13%), Postives = 229/375 (61.07%), Query Frame = 0

Query: 10  DSLSNFTSHLNMALRQKGIDVFIDN-ELSRGEEISASLLKAIEESKISIVIMSENYASSS 69
           D   NF SHL  +LR+ GI  F+D+ EL RGE IS  LL AIE SKI IV+++++YASS+
Sbjct: 27  DVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYASSA 86

Query: 70  WYLNELVKIIMCNKFG-EQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRF-FNKMQEWR 129
           W L+ELV I+  +K     +V P+F  VDPS +R Q G++ + F+K +     NK+++WR
Sbjct: 87  WCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPLNKLKDWR 146

Query: 130 EALTTISNMSGRVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMNL--L 189
           EALT ++N+SG  ++  +EA  I  I +++ K+L      L V  Y VG+  +L ++  L
Sbjct: 147 EALTKVANISGWDIKNRNEAECIADITREILKRL--PCQYLHVPSYAVGLRSRLQHISSL 206

Query: 190 PHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVRLQK 249
             + SDG+ ++ +YG+GG+ KTTLAK  +N  +  FEG  FL + RE S +  G   LQ 
Sbjct: 207 LSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQH 266

Query: 250 DLLREILMDDSIKVSNHHKGIS-IIRHRLCSKKILLILDNVDTHEQLEALPGGHDWFGAG 309
            LL +IL  + I+     KG+   ++ R  SK++LL++D+VD   QL +     D FG G
Sbjct: 267 QLLSDILRRNDIE----FKGLDHAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHG 326

Query: 310 SKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRAVDY 369
           S++I TTRN  LL     +   S  EL+ DE+LELFSWH F+ S P  ++   S+  V Y
Sbjct: 327 SRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTY 386

Query: 370 CKGLPLTLRVLGKFL 379
           C GLPL + VLG FL
Sbjct: 387 CAGLPLAVEVLGAFL 395

BLAST of Lsi03G005860 vs. TAIR 10
Match: AT1G27170.1 (transmembrane receptors;ATP binding )

HSP 1 Score: 263.1 bits (671), Expect = 3.8e-70
Identity = 151/393 (38.42%), Postives = 241/393 (61.32%), Query Frame = 0

Query: 10  DSLSNFTSHLNMALRQKGIDVFIDNE-LSRGEEISASLLKAIEESKISIVIMSENYASSS 69
           D+  NF  HL  AL+ K + VF DNE + RG+EIS+SL   +E+S  S++++S NY+ S 
Sbjct: 24  DTRDNFGDHLYKALKDK-VRVFRDNEGMERGDEISSSLKAGMEDSAASVIVISRNYSGSR 83

Query: 70  WYLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRFF---NKMQEW 129
           W L+EL  +       ++ +LP+FY VDPSHVRKQS    ++F + +VRF     K+QEW
Sbjct: 84  WCLDELAMLCKMKSSLDRRILPIFYHVDPSHVRKQSDHIKKDFEEHQVRFSEEKEKVQEW 143

Query: 130 REALTTISNMSGRVLQKDD-EANLIQIIVQDVWKKLNRATMQLDVAEYPVGID---RQLM 189
           REALT + N++G V  KD  + ++I+++V+ V  +L+    +  V E+ VG++   + L 
Sbjct: 144 REALTLVGNLAGYVCDKDSKDDDMIELVVKRVLAELSNTPEK--VGEFIVGLESPLKDLT 203

Query: 190 NLLPHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLVR 249
            L+    S G+ ++GLYG+GG+ KTTLAKA YN I  +FE   F++DIRE S+   GLV 
Sbjct: 204 GLIDTESSSGVQVLGLYGMGGIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVT 263

Query: 250 LQKDLLREI--LMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDW 309
           LQK L++E+  L+ +   VS    G+  I+  +  KKI+++LD+VD  +Q+ AL G   W
Sbjct: 264 LQKTLIKELFRLVPEIEDVS---IGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRW 323

Query: 310 FGAGSKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKR 369
           +G G+ ++ TTR+ ++L+    +    V  L + +AL+LFS+H+ ++  P+ +   LSK+
Sbjct: 324 YGQGTLIVITTRDSEILSKLSVNQQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKK 383

Query: 370 AVDYCKGLPLTLRVLGKFLCPIDDQSQFESILD 393
            V     LPL + V G  L    ++  +++ LD
Sbjct: 384 IVQISGLLPLAVEVFGSLLYDKKEEKDWQTQLD 410

BLAST of Lsi03G005860 vs. TAIR 10
Match: AT5G58120.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 258.8 bits (660), Expect = 7.2e-69
Identity = 158/397 (39.80%), Postives = 236/397 (59.45%), Query Frame = 0

Query: 10  DSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSSW 69
           D    F SHL       GI +F D  + R + I  +L  AI+ES+ISIV++S+NYASS W
Sbjct: 25  DVRKTFLSHLRKQFSYNGISMFNDQSIERSQTIVPALTGAIKESRISIVVLSKNYASSRW 84

Query: 70  YLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAK-LEVRFFNKMQEWREA 129
            L+EL++I+ C +   Q+V+ VFY VDPS VRKQ+G FG  F K  E +   + Q+W +A
Sbjct: 85  CLDELLEILKCREDIGQIVMTVFYGVDPSDVRKQTGEFGIAFNKTCEGKTNEETQKWSKA 144

Query: 130 LTTISNMSG-RVLQKDDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGID---RQLMNLL 189
           L  + N++G      D+EA +I+ I +DV  KLN AT+  D  E  VGI+   +++ +LL
Sbjct: 145 LNDVGNIAGEHFFNWDNEAKMIEKIARDVSNKLN-ATISWDF-EDMVGIEAHLQKMQSLL 204

Query: 190 PHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESN----QCGGLV 249
                DG  +VG+YG  G+ KTT+A+AL++ ++  F+  CF+ +IR   N    + G  +
Sbjct: 205 HLDYEDGAMIVGIYGPAGIGKTTIARALHSRLSSSFQLTCFMENIRGSYNSGLDEYGLKL 264

Query: 250 RLQKDLLREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWF 309
           RLQ+ LL ++L  D I++ NH   +  I  RLC +K+L+ILD+VD  +QLEAL    +WF
Sbjct: 265 RLQEQLLSKVLNHDGIRI-NH---LGAIPERLCDQKVLIILDDVDDLQQLEALANETNWF 324

Query: 310 GAGSKVIATTRNKQLLASHGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKRA 369
           G GS++I TT +++LL  H  +    VD    +EA ++F  + F+RS+    +  L++R 
Sbjct: 325 GPGSRIIVTTEDQELLEQHDVNKKYHVDFPTREEACKIFCTYAFRRSFAPYGFEKLAERV 384

Query: 370 VDYCKGLPLTLRVLGKFLCPIDDQSQFESILDGYENS 398
              C  LPL LRV+G  L     +  +E IL   ENS
Sbjct: 385 TWLCSNLPLGLRVMGSTLRG-KKEDDWEGILRRLENS 414

BLAST of Lsi03G005860 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 256.1 bits (653), Expect = 4.6e-68
Identity = 152/384 (39.58%), Postives = 226/384 (58.85%), Query Frame = 0

Query: 10  DSLSNFTSHLNMALRQKGIDVFIDNELSRGEEISASLLKAIEESKISIVIMSENYASSSW 69
           D+ +NFT HL  ALR +GID FID+ L RG+ ++A L   IE+SKI+I++ S NYA+S+W
Sbjct: 21  DTRNNFTGHLQKALRLRGIDSFIDDRLRRGDNLTA-LFDRIEKSKIAIIVFSTNYANSAW 80

Query: 70  YLNELVKIIMCNKFGEQVVLPVFYKVDPSHVRKQSGTFGEEFAKLEVRF----FNKMQEW 129
            L ELVKI+ C    +Q+V+P+FYKVD S V KQ  +F   F   E+ F      ++  W
Sbjct: 81  CLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKLPELTFPGVTPEEISSW 140

Query: 130 REALTTISNMSGRVLQK--DDEANLIQIIVQDVWKKLNRATMQLDVAEYPVGIDRQLMN- 189
           + AL + SN+ G V+++    EA L+  I  D +KKLN   +     E  VGI+ +L N 
Sbjct: 141 KAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN--DLAPSGNEGLVGIESRLKNL 200

Query: 190 --LLPHVMSDGISMVGLYGIGGVDKTTLAKALYNIIADDFEGCCFLADIREESNQCGGLV 249
             LL     D + ++G+ G+ G+ KTTLA  LY  +   F+G CFL +IRE S +  GL 
Sbjct: 201 EKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSCFLTNIRENSGR-SGLE 260

Query: 250 RLQKDLLREILMDDSIKVSNHHKGISIIRHRLCSKKILLILDNVDTHEQLEALPGGHDWF 309
            L + L   +L D  +++            RL SK++L++LD+V+  +Q+  L G   W+
Sbjct: 261 SLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVNDEKQIRYLMGHCKWY 320

Query: 310 GAGSKVIATTRNKQLLAS-HGFDILQSVDELNDDEALELFSWHTFKRSYPSSDYFDLSKR 369
             GS++I TTR+ +L+ +  G   +  + +LND EAL+LFS + F  S+P  ++  L+  
Sbjct: 321 QGGSRIIITTRDSKLIETIKGRKYV--LPKLNDREALKLFSLNAFSNSFPLKEFEGLTNM 380

Query: 370 AVDYCKGLPLTLRVLGKFLCPIDD 384
            +DY KG PL L+VLG  LC  DD
Sbjct: 381 VLDYAKGHPLALKVLGSDLCERDD 398

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
V9M2S51.5e-8447.47Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
V9M3982.1e-8145.74Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... [more]
A0A290U7C41.1e-7742.63Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... [more]
Q403921.0e-6740.10TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1[more]
Q9SZ666.5e-6739.58Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=... [more]
Match NameE-valueIdentityDescription
D9I8I55.1e-16374.19Putative TIR-NBS-LRR-AAA+ATPase OS=Cucumis sativus OX=3659 PE=4 SV=1[more]
A0A0A0LGB65.1e-16374.19TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G021540 PE=4 SV... [more]
E7CHD02.5e-16273.93Putative TIR-NBS-LRR-AAA+ATPase OS=Cucumis sativus var. hardwickii OX=319220 PE=... [more]
Q84KB39.5e-16273.50MRGH63 OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3C0Y99.5e-16273.50TMV resistance protein N-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495240... [more]
Match NameE-valueIdentityDescription
XP_038889763.12.3e-17078.37disease resistance protein RPV1-like isoform X1 [Benincasa hispida] >XP_03888976... [more]
XP_038889774.12.3e-17078.37disease resistance protein RPV1-like isoform X2 [Benincasa hispida][more]
ADI99935.11.0e-16274.19TIR-NBS-LRR class resistance protein [Cucumis sativus] >ADU33174.1 putative TIR-... [more]
XP_011650107.21.0e-16274.19TMV resistance protein N [Cucumis sativus] >KGN60913.2 hypothetical protein Csa_... [more]
ADU33175.15.2e-16273.93putative TIR-NBS-LRR-AAA+ATPase [Cucumis sativus var. hardwickii][more]
Match NameE-valueIdentityDescription
AT5G36930.13.1e-7242.13Disease resistance protein (TIR-NBS-LRR class) family [more]
AT5G36930.23.1e-7242.13Disease resistance protein (TIR-NBS-LRR class) family [more]
AT1G27170.13.8e-7038.42transmembrane receptors;ATP binding [more]
AT5G58120.17.2e-6939.80Disease resistance protein (TIR-NBS-LRR class) family [more]
AT4G12010.14.6e-6839.58Disease resistance protein (TIR-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 364..378
score: 51.0
coord: 194..209
score: 55.47
coord: 271..285
score: 47.33
NoneNo IPR availablePANTHERPTHR11017:SF422RESISTANCE PROTEIN (TIR-NBS-LRR CLASS), PUTATIVE-RELATEDcoord: 4..396
IPR000157Toll/interleukin-1 receptor homology (TIR) domainSMARTSM00255till_3coord: 7..135
e-value: 1.7E-32
score: 123.9
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPFAMPF01582TIRcoord: 7..166
e-value: 2.9E-43
score: 147.7
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPROSITEPS50104TIRcoord: 1..160
score: 30.020065
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 166..321
e-value: 1.0E-28
score: 102.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 159..397
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilyGENE3D3.40.50.10140coord: 5..165
e-value: 4.3E-60
score: 204.3
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilySUPERFAMILY52200Toll/Interleukin receptor TIR domaincoord: 8..137
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 329..409
e-value: 5.3E-11
score: 43.9
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 186..389
e-value: 3.2E-22
score: 79.0
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 4..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G005860.1Lsi03G005860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0043531 ADP binding
molecular_function GO:0003953 NAD+ nucleosidase activity