Lsi03G003650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G003650
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	aspartic proteinase PCS1-like
Location	chr03: 4303164 .. 4304453 (+)
RNA-Seq Expression	Lsi03G003650
Synteny	Lsi03G003650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTCTTCTTCCTCCATCTACTGCAACTTCTCATCTATTGTCTATCTTTCAAACAGAGCTTCTGTTTTTCTGCAACTCAGACTATGGTTTTGCCTCTCAAAACACAGATGGGTTTGATTTCTCAGCCTTCTAACAAGCTCAGTTTCCACCATAATGTCACTTTGACTGTTTCCTTAACTGTTGGTTCACCTCCTCAACAAGTCACCATGGTTCTTGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAATCCCCAAATTTGACCTCTGTTTTTAACCCACTTTCCTCTTCTTCTTACTCACCAATCCCCTGTTCTTCCCCCATTTGTCGTACCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCGAAAAAGCTCTGCCACGCCATTGTTTCTTACGCCGATGCATCGTCACTCGAGGGCAATCTCGCATCCGATACCTACCGAATCGGGTCTGCGGCTCAACCCGGAACTTTATTCGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGAGCACTGGATTGATGGGGATGAATAGAGGATCGCTTTCGTTTGTGACCCAATTGGGTTTGCCGAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCAGGGGTTCTTCTTTTCGGCGATGCGCCTCTTTCATGGCTTGGGAATTTAACCTACACGCCTTTAGTTCAAATGTCAACCCCATTGCCTTATTACGACAGAGTCGCCTACACCGTCCAACTCGACGGAATTAGAGTAGGGAACAAAATTCTACCACTCCCGAAATCAATCTTCGCACCAGACCACACCGGCGCCGGGCAAACCATGGTGGATTCAGGAACACAGTTCACGTTTCTTCTCGGACCAGTCTACATAGCTTTAAAGAACGAGTTTCTGACGCAAACGAAAGGAATTTTAGTCCCACTGGGTGATCCAAACTTCGTGTTTCAAGGAGCAATGGACTTGTGCTACAGAGTAGCAGCAAAAGAAGGGAAACTACCACCGTTGCCGGCGGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGAGGGGAGGTGCTGCTGTACAGAGTACCGGAAATGATGAAGGGAAACGAATGGGTGTATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCTTTTGTGATAGGTCATCATCATCAACAAAACGTATGGATGGAATTTGATTTAGTGAAATCAAGGGTGGGATTTGTAGAGACGAGATGTGATTTAGCAGGTCAAAGATTGGGATTGGGCCTTTAA

mRNA sequence

ATGGCCTTCTTCTTCCTCCATCTACTGCAACTTCTCATCTATTGTCTATCTTTCAAACAGAGCTTCTGTTTTTCTGCAACTCAGACTATGGTTTTGCCTCTCAAAACACAGATGGGTTTGATTTCTCAGCCTTCTAACAAGCTCAGTTTCCACCATAATGTCACTTTGACTGTTTCCTTAACTGTTGGTTCACCTCCTCAACAAGTCACCATGGTTCTTGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAATCCCCAAATTTGACCTCTGTTTTTAACCCACTTTCCTCTTCTTCTTACTCACCAATCCCCTGTTCTTCCCCCATTTGTCGTACCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCGAAAAAGCTCTGCCACGCCATTGTTTCTTACGCCGATGCATCGTCACTCGAGGGCAATCTCGCATCCGATACCTACCGAATCGGGTCTGCGGCTCAACCCGGAACTTTATTCGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGAGCACTGGATTGATGGGGATGAATAGAGGATCGCTTTCGTTTGTGACCCAATTGGGTTTGCCGAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCAGGGGTTCTTCTTTTCGGCGATGCGCCTCTTTCATGGCTTGGGAATTTAACCTACACGCCTTTAGTTCAAATGTCAACCCCATTGCCTTATTACGACAGAGTCGCCTACACCGTCCAACTCGACGGAATTAGAGTAGGGAACAAAATTCTACCACTCCCGAAATCAATCTTCGCACCAGACCACACCGGCGCCGGGCAAACCATGGTGGATTCAGGAACACAGTTCACGTTTCTTCTCGGACCAGTCTACATAGCTTTAAAGAACGAGTTTCTGACGCAAACGAAAGGAATTTTAGTCCCACTGGGTGATCCAAACTTCGTGTTTCAAGGAGCAATGGACTTGTGCTACAGAGTAGCAGCAAAAGAAGGGAAACTACCACCGTTGCCGGCGGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGAGGGGAGGTGCTGCTGTACAGAGTACCGGAAATGATGAAGGGAAACGAATGGGTGTATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCTTTTGTGATAGGTCATCATCATCAACAAAACGTATGGATGGAATTTGATTTAGTGAAATCAAGGGTGGGATTTGTAGAGACGAGATGTGATTTAGCAGGTCAAAGATTGGGATTGGGCCTTTAA

Coding sequence (CDS)

ATGGCCTTCTTCTTCCTCCATCTACTGCAACTTCTCATCTATTGTCTATCTTTCAAACAGAGCTTCTGTTTTTCTGCAACTCAGACTATGGTTTTGCCTCTCAAAACACAGATGGGTTTGATTTCTCAGCCTTCTAACAAGCTCAGTTTCCACCATAATGTCACTTTGACTGTTTCCTTAACTGTTGGTTCACCTCCTCAACAAGTCACCATGGTTCTTGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAATCCCCAAATTTGACCTCTGTTTTTAACCCACTTTCCTCTTCTTCTTACTCACCAATCCCCTGTTCTTCCCCCATTTGTCGTACCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCGAAAAAGCTCTGCCACGCCATTGTTTCTTACGCCGATGCATCGTCACTCGAGGGCAATCTCGCATCCGATACCTACCGAATCGGGTCTGCGGCTCAACCCGGAACTTTATTCGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGAGCACTGGATTGATGGGGATGAATAGAGGATCGCTTTCGTTTGTGACCCAATTGGGTTTGCCGAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCAGGGGTTCTTCTTTTCGGCGATGCGCCTCTTTCATGGCTTGGGAATTTAACCTACACGCCTTTAGTTCAAATGTCAACCCCATTGCCTTATTACGACAGAGTCGCCTACACCGTCCAACTCGACGGAATTAGAGTAGGGAACAAAATTCTACCACTCCCGAAATCAATCTTCGCACCAGACCACACCGGCGCCGGGCAAACCATGGTGGATTCAGGAACACAGTTCACGTTTCTTCTCGGACCAGTCTACATAGCTTTAAAGAACGAGTTTCTGACGCAAACGAAAGGAATTTTAGTCCCACTGGGTGATCCAAACTTCGTGTTTCAAGGAGCAATGGACTTGTGCTACAGAGTAGCAGCAAAAGAAGGGAAACTACCACCGTTGCCGGCGGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGAGGGGAGGTGCTGCTGTACAGAGTACCGGAAATGATGAAGGGAAACGAATGGGTGTATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCTTTTGTGATAGGTCATCATCATCAACAAAACGTATGGATGGAATTTGATTTAGTGAAATCAAGGGTGGGATTTGTAGAGACGAGATGTGATTTAGCAGGTCAAAGATTGGGATTGGGCCTTTAA

Protein sequence

MAFFFLHLLQLLIYCLSFKQSFCFSATQTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL

Homology

[Close]

BLAST of Lsi03G003650 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.2e-151
Identity = 271/446 (60.76%), Postives = 335/446 (75.11%), Query Frame = 0


Query: 2   AFFFLHLLQLLIY-CL----SFKQSFCF------SATQTMVLPLKTQMGLIS-QPSNKLS 61
           A F L +L +  Y C+    S   SF F      S++QT+VLPLKT++     +P++KL 
Sbjct: 7   ALFLLLVLSVRTYKCVSSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLH 66

Query: 62  FHHNVTLTVSLTVGSPPQQVTMVLDTGSELSWLHCKKS--PNLTSVFNPLSSSSYSPIPC 121
           FHHNVTLTV+LTVG+PPQ ++MV+DTGSELSWL C +S  PN  + F+P  SSSYSPIPC
Sbjct: 67  FHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPC 126

Query: 122 SSPICRTRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTL-FGC 181
           SSP CRTRTRD   P +CD  KLCHA +SYADASS EGNLA++ +  G++     L FGC
Sbjct: 127 SSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGC 186

Query: 182 MDSGFSSNSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCISGRDS-SGVLLFGDAPLSW 241
           M S   S+ EED K+TGL+GMNRGSLSF++Q+G PKFSYCISG D   G LL GD+  +W
Sbjct: 187 MGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCISGTDDFPGFLLLGDSNFTW 246

Query: 242 LGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSG 301
           L  L YTPL+++STPLPY+DRVAYTVQL GI+V  K+LP+PKS+  PDHTGAGQTMVDSG
Sbjct: 247 LTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSG 306

Query: 302 TQFTFLLGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVA---AKEGKLPPLP 361
           TQFTFLLGPVY AL++ FL +T GIL    DP+FVFQG MDLCYR++    + G L  LP
Sbjct: 307 TQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLP 366

Query: 362 AVSLMFRGAEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWM 421
            VSL+F GAE+ V G+ LLYRVP +  GN+ VYC TFGNSDL+G+EA+VIGHHHQQN+W+
Sbjct: 367 TVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWI 426

Query: 422 EFDLVKSRVGFVETRCDLAGQRLGLG 429
           EFDL +SR+G     CD++GQRLG+G
Sbjct: 427 EFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Lsi03G003650 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.6e-39
Identity = 114/367 (31.06%), Postives = 173/367 (47.14%), Query Frame = 0


Query: 58  VSLTVGSPPQQVTMVLDTGSELSWLHCKKSPNL----TSVFNPLSSSSYSPIPCSSPICR 117
           +++ +G+P    + ++DTGS+L W  C+         T +FNP  SSS+S +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYC- 157

Query: 118 TRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSS 177
              +DLP+  TC+  + C     Y D S+ +G +A++T+   +++ P   FGC   G  +
Sbjct: 158 ---QDLPSE-TCNNNE-CQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGC---GEDN 217

Query: 178 NSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCIS--GRDSSGVLLFGDAPLSWLGNLTY 237
                    GL+GM  G LS  +QLG+ +FSYC++  G  S   L  G A          
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 238 TPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFL 297
           T L+  S    Y     Y + L GI VG   L +P S F     G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 298 LGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGA 357
               Y A+   F  Q   I +P  D +      +  C++    +G    +P +S+ F G 
Sbjct: 338 PQDAYNAVAQAFTDQ---INLPTVDES---SSGLSTCFQ-QPSDGSTVQVPEISMQFDGG 397

Query: 358 EMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRV 417
            + +G + +L      +   E V CL  G+S  LGI  F  G+  QQ   + +DL    V
Sbjct: 398 VLNLGEQNIL------ISPAEGVICLAMGSSSQLGISIF--GNIQQQETQVLYDLQNLAV 435

Query: 418 GFVETRC 419
            FV T+C
Sbjct: 458 SFVPTQC 435

[Close]

BLAST of Lsi03G003650 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.0e-39
Identity = 112/368 (30.43%), Postives = 183/368 (49.73%), Query Frame = 0


Query: 58  VSLTVGSPPQQVTMVLDTGSELSWLHCKKSPNL----TSVFNPLSSSSYSPIPCSSPICR 117
           ++L++G+P Q  + ++DTGS+L W  C+         T +FNP  SSS+S +PCSS +C+
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQ 156

Query: 118 TRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSS 177
                L +P TC     C     Y D S  +G++ ++T   GS + P   FGC   G ++
Sbjct: 157 A----LSSP-TCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC---GENN 216

Query: 178 NSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCIS--GRDSSGVLLFGDAPLSWLGNLTY 237
                    GL+GM RG LS  +QL + KFSYC++  G  +   LL G    S       
Sbjct: 217 QGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN 276

Query: 238 TPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFA-PDHTGAGQTMVDSGTQFTF 297
           T L+Q S+ +P +    Y + L+G+ VG+  LP+  S FA   + G G  ++DSGT  T+
Sbjct: 277 TTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 336

Query: 298 LLGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRG 357
            +   Y +++ EF++Q   I +P+ + +       DLC++  +    L  +P   + F G
Sbjct: 337 FVNNAYQSVRQEFISQ---INLPVVNGS---SSGFDLCFQTPSDPSNL-QIPTFVMHFDG 396

Query: 358 AEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSR 417
            ++ +  E         +  +  + CL  G+S   G+  F  G+  QQN+ + +D   S 
Sbjct: 397 GDLELPSE------NYFISPSNGLICLAMGSSS-QGMSIF--GNIQQQNMLVVYDTGNSV 434

Query: 418 VGFVETRC 419
           V F   +C
Sbjct: 457 VSFASAQC 434

[Close]

BLAST of Lsi03G003650 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 5.8e-34
Identity = 124/371 (33.42%), Postives = 174/371 (46.90%), Query Frame = 0


Query: 60  LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTS----VFNPLSSSSYSPIPCSSPICRTR 119
           L VG+P + V MVLDTGS++ WL C       S    +F+P  S +Y+ IPCSSP CR  
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRR- 205

Query: 120 TRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNS 179
              L +      +K C   VSY D S   G+ +++T         G   GC       N 
Sbjct: 206 ---LDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDNE 265

Query: 180 EEDAKSTGLMGMNRGSLSFVTQLG---LPKFSYCISGRDSS---GVLLFGDAPLSWLGNL 239
                + GL+G+ +G LSF  Q G     KFSYC+  R +S     ++FG+A +S +   
Sbjct: 266 GLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIAR- 325

Query: 240 TYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILP-LPKSIFAPDHTGAGQTMVDSGTQF 299
            +TPL+      P  D   Y V L GI VG   +P +  S+F  D  G G  ++DSGT  
Sbjct: 326 -FTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSV 385

Query: 300 TFLLGPVYIALKNEFLTQTKGILVPLGDPNF-VFQGAMDLCYRVAAKEGKLPPLPAVSLM 359
           T L+ P YIA+++ F    K +      P+F +F    DL      K      +P V L 
Sbjct: 386 TRLIRPAYIAMRDAFRVGAKTL---KRAPDFSLFDTCFDLSNMNEVK------VPTVVLH 445

Query: 360 FRGAEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLV 419
           FRGA+  V      Y +P    G    +C  F  + + G+   +IG+  QQ   + +DL 
Sbjct: 446 FRGAD--VSLPATNYLIPVDTNGK---FCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLA 484

[Close]

BLAST of Lsi03G003650 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.9e-30
Identity = 123/377 (32.63%), Postives = 181/377 (48.01%), Query Frame = 0


Query: 62  VGSPPQQVTMVLDTGSELSWLHCKKSPNL--TSVFNPLSSSSYSPIPCSSPICRT-RTRD 121
           +GSP QQ+ + LDT ++ +W HC        +S+F P +SSSY+ +PCSS  C   + + 
Sbjct: 85  LGSPSQQLLLALDTSADATWAHCSPCGTCPSSSLFAPANSSSYASLPCSSSWCPLFQGQA 144

Query: 122 LPNP-----VTCDPKKL--CHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGF 181
            P P         P  L  C     +ADA S +  LASDT R+G  A P   FGC+ S  
Sbjct: 145 CPAPQGGGDAAPPPATLPTCAFSKPFADA-SFQAALASDTLRLGKDAIPNYTFGCVSS-- 204

Query: 182 SSNSEEDAKSTGLMGMNRGSLSFVTQLGL---PKFSYCISGRDS---SGVLLFGDAPLSW 241
            +    +    GL+G+ RG ++ ++Q G      FSYC+    S   SG L  G A    
Sbjct: 205 VTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLRLG-AGGGQ 264

Query: 242 LGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPD-HTGAGQTMVDS 301
             ++ YTP+++     P+   + Y V + G+ VG+  + +P   FA D  TGAG T+VDS
Sbjct: 265 PRSVRYTPMLRN----PHRSSL-YYVNVTGLSVGHAWVKVPAGSFAFDAATGAG-TVVDS 324

Query: 302 GTQFTFLLGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAV 361
           GT  T    PVY AL+ EF  Q   +  P G   +   GA D C+     E      PAV
Sbjct: 325 GTVITRWTAPVYAALREEFRRQ---VAAPSG---YTSLGAFDTCFN--TDEVAAGGAPAV 384

Query: 362 SL-MFRGAEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNS-DLLGIEAFVIGHHHQQNVWM 420
           ++ M  G ++ +  E  L     +      + CL    +   +     VI +  QQN+ +
Sbjct: 385 TVHMDGGVDLALPMENTL-----IHSSATPLACLAMAEAPQNVNSVVNVIANLQQQNIRV 438

[Close]

BLAST of Lsi03G003650 vs. ExPASy TrEMBL
Match: A0A6J1FDS6 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.5e-226
Identity = 384/425 (90.35%), Postives = 405/425 (95.29%), Query Frame = 0


Query: 4   FFLHLLQLLIYCLSFKQSFCFSATQTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVG 63
           FFL LLQLLI C+SFKQ  CFSATQTMVLPLKTQMG+ S+PSNKLSFHHNVTLTVSLT+G
Sbjct: 3   FFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLG 62

Query: 64  SPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPV 123
           SPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SP+CRTRTRDLPNPV
Sbjct: 63  SPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPV 122

Query: 124 TCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTG 183
           TCDPKKLCH  VSYADASSLEGNLASDT+R+GS+AQPGT FGCMDSGFSSNSEEDAK+TG
Sbjct: 123 TCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTG 182

Query: 184 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPY 243
           LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDA LSWLGNLTYTPLVQMSTPLPY
Sbjct: 183 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPY 242

Query: 244 YDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEF 303
           YDRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY ALKNEF
Sbjct: 243 YDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF 302

Query: 304 LTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEVLLYR 363
           + QTKGILVPLGDPNFVFQGAMDLCYRV  K+GKLPPLP VSLMFRGAEMVVGGEVL+Y+
Sbjct: 303 VVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYK 362

Query: 364 VPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQ 423
           VP M++G + V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAG+
Sbjct: 363 VPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGE 422

Query: 424 RLGLG 429
           RLGLG
Sbjct: 423 RLGLG 427

[Close]

BLAST of Lsi03G003650 vs. ExPASy TrEMBL
Match: A0A0A0K8C7 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008640 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.6e-226
Identity = 396/430 (92.09%), Postives = 406/430 (94.42%), Query Frame = 0


Query: 1   MAFFFLHLLQLLIYCLSFKQSFCFSATQT-MVLPLKTQMGLISQPSNKLSFHHNVTLTVS 60
           MAFFF      L+ CLS KQS CFSAT T MVLPL TQMGLISQPSNKLSFHHNVTLTVS
Sbjct: 1   MAFFFFF---FLLVCLSLKQSLCFSATPTSMVLPLNTQMGLISQPSNKLSFHHNVTLTVS 60

Query: 61  LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDL 120
           LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDL
Sbjct: 61  LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDL 120

Query: 121 PNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDA 180
           PNPVTCDPKKLCHAIVSYADASSLEGNLASD +RIGS+A PGTLFGCMDSGFSSNSEEDA
Sbjct: 121 PNPVTCDPKKLCHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDA 180

Query: 181 KSTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMST 240
           K+TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGD  LSWLGNLTYTPLVQ+ST
Sbjct: 181 KTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDLHLSWLGNLTYTPLVQIST 240

Query: 241 PLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIAL 300
           PLPY+DRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY AL
Sbjct: 241 PLPYFDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL 300

Query: 301 KNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEV 360
           +NEFL QTKG+L PLGDPNFVFQGAMDLCY VAA  GKLP LP+VSLMFRGAEMVVGGEV
Sbjct: 301 RNEFLEQTKGVLAPLGDPNFVFQGAMDLCYSVAA-GGKLPTLPSVSLMFRGAEMVVGGEV 360

Query: 361 LLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD 420
           LLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD
Sbjct: 361 LLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD 420

Query: 421 LAGQRLGLGL 430
           LA QRLG+GL
Sbjct: 421 LASQRLGVGL 426

[Close]

BLAST of Lsi03G003650 vs. ExPASy TrEMBL
Match: A0A6J1JZR1 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.4e-226
Identity = 384/425 (90.35%), Postives = 404/425 (95.06%), Query Frame = 0


Query: 4   FFLHLLQLLIYCLSFKQSFCFSATQTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVG 63
           FFL LL LLI C+SFKQS CFSA QTMVLPLKTQMG+ SQPSNKLSFHHNVTLTVSLT+G
Sbjct: 3   FFLRLLHLLICCVSFKQSLCFSAIQTMVLPLKTQMGVTSQPSNKLSFHHNVTLTVSLTLG 62

Query: 64  SPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPV 123
           SPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SP+CRTRTRDLPNPV
Sbjct: 63  SPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPV 122

Query: 124 TCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTG 183
           TCDPKKLCH  VSYADASSLEGNLASDT+R+GS+AQPGT FGCMDSGFSSNSEEDAK+TG
Sbjct: 123 TCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTG 182

Query: 184 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPY 243
           LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDA LSWLGNLTYTPLVQMSTPLPY
Sbjct: 183 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPY 242

Query: 244 YDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEF 303
           YDRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY ALKNEF
Sbjct: 243 YDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF 302

Query: 304 LTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEVLLYR 363
           + QTKGILVPLGDPNFVFQGAMDLCYRV  K+GKLPPLP VSLMFRGAEMVVGGEVL+YR
Sbjct: 303 VVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYR 362

Query: 364 VPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQ 423
           VP M++G + V+C+TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAG+
Sbjct: 363 VPGMVRGGDQVHCVTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGE 422

Query: 424 RLGLG 429
           RLGLG
Sbjct: 423 RLGLG 427

[Close]

BLAST of Lsi03G003650 vs. ExPASy TrEMBL
Match: A0A1S3B3Y8 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103485512 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.3e-225
Identity = 393/430 (91.40%), Postives = 406/430 (94.42%), Query Frame = 0


Query: 1   MAFFFLHLLQLLIYCLSFKQSFCFSAT-QTMVLPLKTQMGLISQPSNKLSFHHNVTLTVS 60
           M+FFF      L+  LS KQS CFSAT  TMVLPL+TQMGLISQPSNKLSFHHNVTLTVS
Sbjct: 1   MSFFFFFF--FLVVSLSMKQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVS 60

Query: 61  LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDL 120
           LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSP+CRTRTRDL
Sbjct: 61  LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDL 120

Query: 121 PNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDA 180
           PNPVTCDPKKLCHAIVSYADASSLEGNLASD +RIGS+A PGTLFGCMDSGFSSNSEEDA
Sbjct: 121 PNPVTCDPKKLCHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDA 180

Query: 181 KSTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMST 240
           K+TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGD+ LSWLGNLTYTPLVQ+ST
Sbjct: 181 KTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQIST 240

Query: 241 PLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIAL 300
           PLPY+DRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY AL
Sbjct: 241 PLPYFDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL 300

Query: 301 KNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEV 360
           +NEFL QTKG+L PLGDPNFVFQGAMDLCYRV A  GKLP LPAVSLMFRGAEMVVGGEV
Sbjct: 301 RNEFLEQTKGVLAPLGDPNFVFQGAMDLCYRVPA-GGKLPELPAVSLMFRGAEMVVGGEV 360

Query: 361 LLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD 420
           LLY+VP MMKG EWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD
Sbjct: 361 LLYKVPGMMKGKEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD 420

Query: 421 LAGQRLGLGL 430
           LAGQRLGLGL
Sbjct: 421 LAGQRLGLGL 427

[Close]

BLAST of Lsi03G003650 vs. ExPASy TrEMBL
Match: A0A5D3DLY6 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00670 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 9.2e-224
Identity = 386/412 (93.69%), Postives = 397/412 (96.36%), Query Frame = 0


Query: 19  KQSFCFSAT-QTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 78
           KQS CFSAT  TMVLPL+TQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 79  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPVTCDPKKLCHAIVSY 138
           ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSP+CRTRTRDLPNPVTCDPKKLCHAIVSY
Sbjct: 62  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVSY 121

Query: 139 ADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTGLMGMNRGSLSFVTQ 198
           ADASSLEGNLASD +RIGS+A PGTLFGCMDSGFSSNSEEDAK+TGLMGMNRGSLSFVTQ
Sbjct: 122 ADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 181

Query: 199 LGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIR 258
           LGLPKFSYCISGRDSSGVLLFGD+ LSWLGNLTYTPLVQ+STPLPY+DRVAYTVQLDGIR
Sbjct: 182 LGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGIR 241

Query: 259 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEFLTQTKGILVPLGDP 318
           VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY AL+NEFL QTKG+L PLGDP
Sbjct: 242 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGDP 301

Query: 319 NFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEVLLYRVPEMMKGNEWVYCL 378
           NFVFQGAMDLCYRV A  GKLP LPAVSLMFRGAEMVVGGEVLLY+VP MMKG EWVYCL
Sbjct: 302 NFVFQGAMDLCYRVPA-GGKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYCL 361

Query: 379 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL 430
           TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL
Sbjct: 362 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL 412

[Close]

BLAST of Lsi03G003650 vs. NCBI nr
Match: XP_038883963.1 (aspartic proteinase PCS1-like [Benincasa hispida])

HSP 1 Score: 820.1 bits (2117), Expect = 9.1e-234
Identity = 408/431 (94.66%), Postives = 416/431 (96.52%), Query Frame = 0


Query: 1   MAFFF-LHLLQLLIYCLSFKQSFCFSATQ-TMVLPLKTQMGLISQPSNKLSFHHNVTLTV 60
           MAFFF L LLQLLIY LS KQS CFSATQ T+VLPLKTQMGLISQPSNKLSFHHNVTLTV
Sbjct: 1   MAFFFLLRLLQLLIYGLSLKQSLCFSATQMTLVLPLKTQMGLISQPSNKLSFHHNVTLTV 60

Query: 61  SLTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRD 120
           SLTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRD
Sbjct: 61  SLTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRD 120

Query: 121 LPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEED 180
           LPNPV CDPKKLCH  VSYADASSLEGNLASDT+RIGS+AQPGTLFGCMDSGFSSNSEED
Sbjct: 121 LPNPVICDPKKLCHVFVSYADASSLEGNLASDTFRIGSSAQPGTLFGCMDSGFSSNSEED 180

Query: 181 AKSTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMS 240
           AK+TGLMGMNRGSLSFVTQLGLPKFSYCISG DSSGVLLFGDAP+SWLGNLTYTPLVQ+S
Sbjct: 181 AKTTGLMGMNRGSLSFVTQLGLPKFSYCISGSDSSGVLLFGDAPVSWLGNLTYTPLVQIS 240

Query: 241 TPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIA 300
           TPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY A
Sbjct: 241 TPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTA 300

Query: 301 LKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGE 360
           LKNEFL QTKG+L+PLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMF GAEMVVGGE
Sbjct: 301 LKNEFLEQTKGVLIPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFHGAEMVVGGE 360

Query: 361 VLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRC 420
           VLLYRVP MMKGNE VYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRC
Sbjct: 361 VLLYRVPGMMKGNELVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRC 420

Query: 421 DLAGQRLGLGL 430
           DLAGQRLGLGL
Sbjct: 421 DLAGQRLGLGL 431

[Close]

BLAST of Lsi03G003650 vs. NCBI nr
Match: XP_022938661.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 795.0 bits (2052), Expect = 3.1e-226
Identity = 384/425 (90.35%), Postives = 405/425 (95.29%), Query Frame = 0


Query: 4   FFLHLLQLLIYCLSFKQSFCFSATQTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVG 63
           FFL LLQLLI C+SFKQ  CFSATQTMVLPLKTQMG+ S+PSNKLSFHHNVTLTVSLT+G
Sbjct: 3   FFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLG 62

Query: 64  SPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPV 123
           SPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SP+CRTRTRDLPNPV
Sbjct: 63  SPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPV 122

Query: 124 TCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTG 183
           TCDPKKLCH  VSYADASSLEGNLASDT+R+GS+AQPGT FGCMDSGFSSNSEEDAK+TG
Sbjct: 123 TCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTG 182

Query: 184 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPY 243
           LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDA LSWLGNLTYTPLVQMSTPLPY
Sbjct: 183 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPY 242

Query: 244 YDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEF 303
           YDRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY ALKNEF
Sbjct: 243 YDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF 302

Query: 304 LTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEVLLYR 363
           + QTKGILVPLGDPNFVFQGAMDLCYRV  K+GKLPPLP VSLMFRGAEMVVGGEVL+Y+
Sbjct: 303 VVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYK 362

Query: 364 VPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQ 423
           VP M++G + V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAG+
Sbjct: 363 VPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGE 422

Query: 424 RLGLG 429
           RLGLG
Sbjct: 423 RLGLG 427

[Close]

BLAST of Lsi03G003650 vs. NCBI nr
Match: XP_011656388.1 (aspartic proteinase PCS1 [Cucumis sativus] >KGN45733.1 hypothetical protein Csa_004908 [Cucumis sativus])

HSP 1 Score: 794.3 bits (2050), Expect = 5.3e-226
Identity = 396/430 (92.09%), Postives = 406/430 (94.42%), Query Frame = 0


Query: 1   MAFFFLHLLQLLIYCLSFKQSFCFSATQT-MVLPLKTQMGLISQPSNKLSFHHNVTLTVS 60
           MAFFF      L+ CLS KQS CFSAT T MVLPL TQMGLISQPSNKLSFHHNVTLTVS
Sbjct: 1   MAFFFFF---FLLVCLSLKQSLCFSATPTSMVLPLNTQMGLISQPSNKLSFHHNVTLTVS 60

Query: 61  LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDL 120
           LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDL
Sbjct: 61  LTVGSPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDL 120

Query: 121 PNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDA 180
           PNPVTCDPKKLCHAIVSYADASSLEGNLASD +RIGS+A PGTLFGCMDSGFSSNSEEDA
Sbjct: 121 PNPVTCDPKKLCHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDA 180

Query: 181 KSTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMST 240
           K+TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGD  LSWLGNLTYTPLVQ+ST
Sbjct: 181 KTTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDLHLSWLGNLTYTPLVQIST 240

Query: 241 PLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIAL 300
           PLPY+DRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY AL
Sbjct: 241 PLPYFDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL 300

Query: 301 KNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEV 360
           +NEFL QTKG+L PLGDPNFVFQGAMDLCY VAA  GKLP LP+VSLMFRGAEMVVGGEV
Sbjct: 301 RNEFLEQTKGVLAPLGDPNFVFQGAMDLCYSVAA-GGKLPTLPSVSLMFRGAEMVVGGEV 360

Query: 361 LLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD 420
           LLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD
Sbjct: 361 LLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCD 420

Query: 421 LAGQRLGLGL 430
           LA QRLG+GL
Sbjct: 421 LASQRLGVGL 426

[Close]

BLAST of Lsi03G003650 vs. NCBI nr
Match: XP_022992613.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 793.9 bits (2049), Expect = 6.9e-226
Identity = 384/425 (90.35%), Postives = 404/425 (95.06%), Query Frame = 0


Query: 4   FFLHLLQLLIYCLSFKQSFCFSATQTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVG 63
           FFL LL LLI C+SFKQS CFSA QTMVLPLKTQMG+ SQPSNKLSFHHNVTLTVSLT+G
Sbjct: 3   FFLRLLHLLICCVSFKQSLCFSAIQTMVLPLKTQMGVTSQPSNKLSFHHNVTLTVSLTLG 62

Query: 64  SPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPV 123
           SPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SP+CRTRTRDLPNPV
Sbjct: 63  SPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPNPV 122

Query: 124 TCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTG 183
           TCDPKKLCH  VSYADASSLEGNLASDT+R+GS+AQPGT FGCMDSGFSSNSEEDAK+TG
Sbjct: 123 TCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTG 182

Query: 184 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPY 243
           LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDA LSWLGNLTYTPLVQMSTPLPY
Sbjct: 183 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPY 242

Query: 244 YDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEF 303
           YDRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY ALKNEF
Sbjct: 243 YDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF 302

Query: 304 LTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEVLLYR 363
           + QTKGILVPLGDPNFVFQGAMDLCYRV  K+GKLPPLP VSLMFRGAEMVVGGEVL+YR
Sbjct: 303 VVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYR 362

Query: 364 VPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQ 423
           VP M++G + V+C+TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAG+
Sbjct: 363 VPGMVRGGDQVHCVTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGE 422

Query: 424 RLGLG 429
           RLGLG
Sbjct: 423 RLGLG 427

[Close]

BLAST of Lsi03G003650 vs. NCBI nr
Match: KAG7015977.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 790.8 bits (2041), Expect = 5.9e-225
Identity = 382/425 (89.88%), Postives = 403/425 (94.82%), Query Frame = 0


Query: 4   FFLHLLQLLIYCLSFKQSFCFSATQTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVG 63
           FFL LLQLLI C+SFKQ  CFSATQTMVLPLKTQMG+ S+PSNKLSFHHNVTLTVSLT+G
Sbjct: 3   FFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLG 62

Query: 64  SPPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPV 123
           SPPQ VTMVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SP+CR RTRDLPNPV
Sbjct: 63  SPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPNPV 122

Query: 124 TCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTG 183
           TCDPKKLCH  VSYADASSLEGNLASDT+R+GS+AQPGT FGCMDSGFSSNSEEDAK+TG
Sbjct: 123 TCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTG 182

Query: 184 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPY 243
           LMGMNRGSLSFVTQ GLPKFSYCISGRDSSGVLLFGDA LSWLGNLTYTPLVQMSTPLPY
Sbjct: 183 LMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPY 242

Query: 244 YDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEF 303
           YDRVAYTVQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVY ALKNEF
Sbjct: 243 YDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEF 302

Query: 304 LTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLPPLPAVSLMFRGAEMVVGGEVLLYR 363
           + QTKGILVPLGDPNFVFQGAMDLCYRV  K+GKLPPLP VSLMFRGAEMVVGGEVL+Y+
Sbjct: 303 VVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYK 362

Query: 364 VPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQ 423
           VP M++G + V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAG+
Sbjct: 363 VPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGE 422

Query: 424 RLGLG 429
           RLGLG
Sbjct: 423 RLGLG 427

[Close]

BLAST of Lsi03G003650 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 608.6 bits (1568), Expect = 3.9e-174
Identity = 309/427 (72.37%), Postives = 344/427 (80.56%), Query Frame = 0


Query: 7   HLLQLLIYCLSFKQSFC--FSATQTMVLPLKTQMGLISQPSNKLSFHHNVTLTVSLTVGS 66
           + L++ +  L F  +FC   S  QT++  LKTQ  L    S+KLSF HNVTLTV+L VG 
Sbjct: 15  NFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQK-LPQSSSDKLSFRHNVTLTVTLAVGD 74

Query: 67  PPQQVTMVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPICRTRTRDLPNPVT 126
           PPQ ++MVLDTGSELSWLHCKKSPNL SVFNP+SSS+YSP+PCSSPICRTRTRDLP P +
Sbjct: 75  PPQNISMVLDTGSELSWLHCKKSPNLGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIPAS 134

Query: 127 CDPK-KLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTLFGCMDSGFSSNSEEDAKSTG 186
           CDPK  LCH  +SYADA+S+EGNLA +T+ IGS  +PGTLFGCMDSG SSNSEEDAKSTG
Sbjct: 135 CDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKSTG 194

Query: 187 LMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDAPLSWLGNLTYTPLVQMSTPLPY 246
           LMGMNRGSLSFV QLG  KFSYCISG DSSG LL GDA  SWLG + YTPLV  STPLPY
Sbjct: 195 LMGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPLPY 254

Query: 247 YDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEF 306
           +DRVAYTVQL+GIRVG+KIL LPKS+F PDHTGAGQTMVDSGTQFTFL+GPVY ALKNEF
Sbjct: 255 FDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEF 314

Query: 307 LTQTKGILVPLGDPNFVFQGAMDLCYRV-AAKEGKLPPLPAVSLMFRGAEMVVGGEVLLY 366
           +TQTK +L  + DP+FVFQG MDLCY+V +        LP VSLMFRGAEM V G+ LLY
Sbjct: 315 ITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKLLY 374

Query: 367 RVPEM-MKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFV-ETRCDL 426
           RV     +G E VYC TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGF    RCDL
Sbjct: 375 RVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRCDL 434

Query: 427 AGQRLGL 428
           A QRLGL
Sbjct: 435 ASQRLGL 440

[Close]

BLAST of Lsi03G003650 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 537.7 bits (1384), Expect = 8.4e-153
Identity = 271/446 (60.76%), Postives = 335/446 (75.11%), Query Frame = 0


Query: 2   AFFFLHLLQLLIY-CL----SFKQSFCF------SATQTMVLPLKTQMGLIS-QPSNKLS 61
           A F L +L +  Y C+    S   SF F      S++QT+VLPLKT++     +P++KL 
Sbjct: 7   ALFLLLVLSVRTYKCVSSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLH 66

Query: 62  FHHNVTLTVSLTVGSPPQQVTMVLDTGSELSWLHCKKS--PNLTSVFNPLSSSSYSPIPC 121
           FHHNVTLTV+LTVG+PPQ ++MV+DTGSELSWL C +S  PN  + F+P  SSSYSPIPC
Sbjct: 67  FHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPC 126

Query: 122 SSPICRTRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSAAQPGTL-FGC 181
           SSP CRTRTRD   P +CD  KLCHA +SYADASS EGNLA++ +  G++     L FGC
Sbjct: 127 SSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGC 186

Query: 182 MDSGFSSNSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCISGRDS-SGVLLFGDAPLSW 241
           M S   S+ EED K+TGL+GMNRGSLSF++Q+G PKFSYCISG D   G LL GD+  +W
Sbjct: 187 MGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCISGTDDFPGFLLLGDSNFTW 246

Query: 242 LGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSG 301
           L  L YTPL+++STPLPY+DRVAYTVQL GI+V  K+LP+PKS+  PDHTGAGQTMVDSG
Sbjct: 247 LTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSG 306

Query: 302 TQFTFLLGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVA---AKEGKLPPLP 361
           TQFTFLLGPVY AL++ FL +T GIL    DP+FVFQG MDLCYR++    + G L  LP
Sbjct: 307 TQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLP 366

Query: 362 AVSLMFRGAEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWM 421
            VSL+F GAE+ V G+ LLYRVP +  GN+ VYC TFGNSDL+G+EA+VIGHHHQQN+W+
Sbjct: 367 TVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWI 426

Query: 422 EFDLVKSRVGFVETRCDLAGQRLGLG 429
           EFDL +SR+G     CD++GQRLG+G
Sbjct: 427 EFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Lsi03G003650 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.5 bits (615), Expect = 1.2e-63
Identity = 149/403 (36.97%), Postives = 212/403 (52.61%), Query Frame = 0


Query: 42  SQPSNKLSFHHNV----TLTVSLTVGSPPQQVTMVLDTGSELSWLHC------KKSPNLT 101
           S PS+  +F  N+     L +SL +G+P Q   +VLDTGS+LSW+ C      K  P  T
Sbjct: 62  SPPSSPYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPT 121

Query: 102 SVFNPLSSSSYSPIPCSSPICRTRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDT 161
           + F+P  SSS+S +PCS P+C+ R  D   P +CD  +LCH    YAD +  EGNL  + 
Sbjct: 122 TSFDPSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEK 181

Query: 162 YRI-GSAAQPGTLFGCMDSGFSSNSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCISGR 221
           +    S   P  + GC        ++E     G++GMN G LSF++Q  + KFSYCI  R
Sbjct: 182 FTFSNSQTTPPLILGC--------AKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTR 241

Query: 222 D------SSGVLLFGDAPLSWLGNLTYTPLV--QMSTPLPYYDRVAYTVQLDGIRVGNKI 281
                  S+G    GD P S      Y  L+    S  +P  D +AYTV L GIR+G K 
Sbjct: 242 SNRPGLASTGSFYLGDNPNS--RGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKR 301

Query: 282 LPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEFLTQTKGILVPLGDPNFVFQ 341
           L +P S+F PD  G+GQTMVDSG++FT L+   Y  +K E +      L       +V+ 
Sbjct: 302 LNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRL----KKGYVYG 361

Query: 342 GAMDLCY--RVAAKEGKLPPLPAVSLMF---RGAEMVVGGEVLLYRVPEMMKGNEWVYCL 401
              D+C+    + + G+L       L+F   RG E++V  + LL  V         ++C+
Sbjct: 362 STADMCFDGNHSMEIGRL----IGDLVFEFGRGVEILVEKQSLLVNV------GGGIHCV 421

Query: 402 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDL 421
             G S +LG  + +IG+ HQQN+W+EFD+   RVGF +  C L
Sbjct: 422 GIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

[Close]

BLAST of Lsi03G003650 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-63
Identity = 146/395 (36.96%), Postives = 210/395 (53.16%), Query Frame = 0


Query: 42  SQPSN-KLSFHHNVTLTVSLTVGSPPQQVTMVLDTGSELSWLHC---KKSPNLTSVFNPL 101
           S P N +  F +++ L +SL +G+PPQ   MVLDTGS+LSW+ C   K  P   + F+P 
Sbjct: 57  SPPYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTSFDPS 116

Query: 102 SSSSYSPIPCSSPICRTRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRIGSA 161
            SSS+S +PCS P+C+ R  D   P +CD  +LCH    YAD +  EGNL  +     + 
Sbjct: 117 LSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNT 176

Query: 162 -AQPGTLFGCMDSGFSSNSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCI------SGR 221
              P  + GC        + E +   G++GMNRG LSFV+Q  + KFSYCI       G 
Sbjct: 177 EITPPLILGC--------ATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGF 236

Query: 222 DSSGVLLFGDAPLSWLGNLTYTPLVQM--STPLPYYDRVAYTVQLDGIRVGNKILPLPKS 281
             +G    GD P S      Y  L+    S  +P  D +AYTV + GIR G K L +  S
Sbjct: 237 TPTGSFYLGDNPNS--HGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGS 296

Query: 282 IFAPDHTGAGQTMVDSGTQFTFLLGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLC 341
           +F PD  G+GQTMVDSG++FT L+   Y  ++ E +T+    L       +V+ G  D+C
Sbjct: 297 VFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRL----KKGYVYGGTADMC 356

Query: 342 YRVAAKEGKLPPLP-----AVSLMFRGAEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSD 401
           +     +G +  +P      V +  RG E++V  E +L  V         ++C+  G S 
Sbjct: 357 F-----DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNV------GGGIHCVGIGRSS 416

Query: 402 LLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRC 419
           +LG  + +IG+ HQQN+W+EFD+   RVGF +  C
Sbjct: 417 MLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

[Close]

BLAST of Lsi03G003650 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.8e-38
Identity = 120/379 (31.66%), Postives = 184/379 (48.55%), Query Frame = 0


Query: 58  VSLTVGSPPQQVTMVLDTGSELSWLHCKKSPNL----TSVFNPLSSSSYSPIPCSSPICR 117
           + L++G+P  + + ++DTGS+L W  CK         T +F+P  SSSYS + CSS +C 
Sbjct: 109 MELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCN 168

Query: 118 TRTRDLPNPVTCDPKKLCHAIVSYADASSLEGNLASDTYRI-GSAAQPGTLFGCMDSGFS 177
                LP     + K  C  + +Y D SS  G LA++T+      +  G  FGC   G  
Sbjct: 169 A----LPRSNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGC---GVE 228

Query: 178 SNSEEDAKSTGLMGMNRGSLSFVTQLGLPKFSYCIS-------------GRDSSGVLLFG 237
           +  +  ++ +GL+G+ RG LS ++QL   KFSYC++             G  +SG++   
Sbjct: 229 NEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKT 288

Query: 238 DAPLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILPLPKSIFAPDHTGAGQ 297
            A L   G +T T  +  +   P +    Y ++L GI VG K L + KS F     G G 
Sbjct: 289 GASLD--GEVTKTMSLLRNPDQPSF----YYLELQGITVGAKRLSVEKSTFELAEDGTGG 348

Query: 298 TMVDSGTQFTFLLGPVYIALKNEFLTQTKGILVPLGDPNFVFQGAMDLCYRVAAKEGKLP 357
            ++DSGT  T+L    +  LK EF   T  + +P+ D        +DLC+++     K  
Sbjct: 349 MIIDSGTTITYLEETAFKVLKEEF---TSRMSLPVDDSG---STGLDLCFKL-PDAAKNI 408

Query: 358 PLPAVSLMFRGAEMVVGGEVLLYRVPEMMKGNEWVYCLTFGNSDLLGIEAFVIGHHHQQN 417
            +P +   F+GA++ + GE   Y V +   G   V CL  G+S+ + I     G+  QQN
Sbjct: 409 AVPKMIFHFKGADLELPGE--NYMVADSSTG---VLCLAMGSSNGMSI----FGNVQQQN 458

Query: 418 VWMEFDLVKSRVGFVETRC 419
             +  DL K  V FV T C
Sbjct: 469 FNVLHDLEKETVSFVPTEC 458

The following BLAST results are available for this feature:

BLAST of Lsi03G003650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	1.2e-151	60.76	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C2	4.6e-39	31.06	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	6.0e-39	30.43	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LNJ3	5.8e-34	33.42	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q6F4N5	3.9e-30	32.63	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]

BLAST of Lsi03G003650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FDS6	1.5e-226	90.35	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3...	[more]
A0A0A0K8C7	2.6e-226	92.09	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G00864...	[more]
A0A6J1JZR1	3.4e-226	90.35	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 S...	[more]
A0A1S3B3Y8	8.3e-225	91.40	aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103485512 PE=3 SV=1	[more]
A0A5D3DLY6	9.2e-224	93.69	Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]

BLAST of Lsi03G003650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883963.1	9.1e-234	94.66	aspartic proteinase PCS1-like [Benincasa hispida]	[more]
XP_022938661.1	3.1e-226	90.35	aspartic proteinase PCS1-like [Cucurbita moschata]	[more]
XP_011656388.1	5.3e-226	92.09	aspartic proteinase PCS1 [Cucumis sativus] >KGN45733.1 hypothetical protein Csa_...	[more]
XP_022992613.1	6.9e-226	90.35	aspartic proteinase PCS1-like [Cucurbita maxima]	[more]
KAG7015977.1	5.9e-225	89.88	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lsi03G003650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39710.1	3.9e-174	72.37	Eukaryotic aspartyl protease family protein	[more]
AT5G02190.1	8.4e-153	60.76	Eukaryotic aspartyl protease family protein	[more]
AT5G37540.1	1.2e-63	36.97	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	1.6e-63	36.96	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	1.8e-38	31.66	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 57..220 e-value: 3.7E-38 score: 131.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 48..219 e-value: 6.3E-36 score: 126.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 225..421 e-value: 4.1E-42 score: 145.9
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 56..418
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 248..414 e-value: 3.5E-35 score: 121.2
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 7..427
None	No IPR available	PANTHER	PTHR47965:SF49	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 7..427
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 71..82
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 56..414 score: 34.456856
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 55..418 e-value: 2.01785E-71 score: 224.064

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G003650.1	Lsi03G003650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity