Lsi03G002510 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G002510
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein NETWORKED 4A
Locationchr03: 3159937 .. 3162742 (+)
RNA-Seq ExpressionLsi03G002510
SyntenyLsi03G002510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAGAAGAAGCCAGAATTTGAAAAATGGAATCTTAGTAGTTATCGTTATTTCCACCATTTTGATTATTAAAGTGTCATTTAGTCCCCTGTCTCTCTCTACGCTCTCTGTTACTCTAATCTGTTCTTACTCTTTCAAAACTTTTCTCATTGTCCTCTACGTTTCTTCCCTCTCAATCCGCCCCCACTTTCCCCCAATCAATCACTCTCGCTTTTCAACTTGCTCTCTTCCGATTGAATTCCAGATCTTTCTGCGCTCTTCATCGATTTCCAACTTCATCAGGTACTGCCTCCTCTCCTTTCCTTTCCTTTTTCTAATTTGTTCCAAGGATTGGTTCGTCTTGTGTGTTGTTTTGGAGTAGATGCTTCGGTAATGATTTTAAGCTGCGAAGATTTCTTGATGAATTGCATTTCAGTTGTGTGGTTTTGTTGATGGATTCTGCATATTTGATTCTACTACCTCCTCTGCTTTCCATTTTCTAATTTGTTCCAAGGATTGGTTCGTCTTATGTGTTGTTTTGGAGTAGATGCTTCGGTGATTTCTTTAAGCTGCGAAGATTTCTTTTGGTTTGGATCTTCTTTATGAAATTCGTTTCAGATGTGTGATTGTATTTCTAAATTTCTAGATGTACTTGATTAATTTGTACTATACGTCCAGGAAGATGTTGTTCAAGTTTCTTCTTCGAACTCTGAGTTGATGTATTTTCCTGATTGAAGGCTTTCCTGCAGATTTTTGGATCGGTGCTTGTGTTCGTATGGAAAATCCGGAATTGAAGAAGCTGAAACCATTTTGGCCTGAGGCTGACATTCAGAACAGATCTAAATGGCTATCAAATAGTCTTGAAGGTAAGTAGTATTTATGATTGAATTTTCTGGTTCCTTGTCCAAGCCTATCGTAACAAGCCTTTCATTCTTTAGTGGAAGGAAGTGACAACTTGTTATTAAATCAAATCGCTTTCTACTTGTACTGAACCCTCTTCTATTACTTCTCTCGTTATCTCTAAGATTGATTTGTGCTCCGAGTAATGTCACACAAAATTTTGGAAATTACTAAATTAATCCGAGAGGTTTTTCTCCGACAGAAATGAATCGGAGTGTTGAGCAGATGCTGAAGGTGATCAAAGAAAATAGTGATAATTTCCCGAAGAGTGCTGATATGGACTCTCAAGTTGAGGAGTTCTCACGCTTGTATCAATCATTGGTTGAAAATGTGTTATCGCCGGAGCTACAATTGCAGGTGCCCGTATATTCTGATTGTGGATCTCCTCAAGGCACTCCGGAATTAAGTTCGGACCAAAAACAGGGTTTCAATTTGTCAAGCAATAGAGGCTTGGATATCTCTTTTGATTCTGGTGGTGGTAGCTCCAGTCTTTCTCTGAAGGATGGAACTGAATCTTCTTCTTCTTCTTCTTCTTCAGACTCGGAGTCAGAATCTTTCAACTCATCTGTCGACAATAACTATGTGGTTTCACGTGCTGAAAGAGATGGTCAAGGGCTGAAGAAAAAACTTCTTGGGATAGAAACTGGGCTTCCAAACATTAAAGGGGCGTTTTGGGTGGGTGAGGAAGACAAGGTGGATTATGATGAGCTACATGACAAACTCGCCAGGATTGAGGAAGAACTAAAAGTTTCGAATACAAAGCTTCAGTCATCTGAAAATGAGGTAGCTAGATTAAAGAGTGAGGTTGAGAAGAATGAAACAGCAATTTTGCTATCTGAAGGACTGCAGGCCCAGCTTGAGTCAGCTGAAAAAGATAAGCAAGAAATGGAGGCTAACCTTCAAGTGGAAAAAAGGCGAGTTGTGGAATTGGAACAACAAATAGAACAATTGGAAACTCGGGTTTTACAATCTGATTCAAAAGTTGAGAGATTGATTGAAGAATTAGAAATAAGCAAAGAAATCCTCAAAAGTTCAGATGACAAGATCACAAGGCTTACTCATGAACTCGAGATTACAAAATCTGACCACCATCTACAGATCAAGGAATTAGAAACTGCTTTTCAAGTTTCACAGGAGAGATTTCATGCTGAGAAAGAACAGATGCAGACTGACATTCTCAGACAAGTTGAAGCTGAGAAAACCGAAACGACAGCTCTCCACAACTCTCATTTGACAATTTTGCAAGGAGAGATCAGTCAGTTGAAGGAAGAACTTTCTGTAAGAAGCGAAAGTTTAGCAGCTTTGAATAGAAACCACGACGAACTAAAACTAAAATATGATATGCTAATGGCTGAGAAAGATGAAAGGAATGCCATGGTTCACTCACTTTTAGCAGATAAAGAATCCAGAGAATCTCATGTCCATGAATTGGAGGATCACCTGCAACATCTACAAGTAGAGAAGAAGAGCTTGATTGAAGGATCTGAAAGGCAGAACAAACAAATTGATGACTTGAAATTGAGACTCTCAGAATTGGAAGAAGAAGTGAACATGCAAAAGGGTATGATAGAGGATGGAGCTGAGGGGAAAAGGGAGGCCATAAGGCAGCTCTGTTTCTCTTTGGAGCATTACAGGAGTGGCTATCAAGAGCTTCGTGAAGCATTCATTGGGCACAAGCCGCGGCCGGTACTCTCTACATAGGTCTGTTTGCTTGTAAAATCTCTTAAGCTTATTACCATGTCTGTTTTTGTGCATTTTTATGAGTATTATGAGCCATTATTTAGTTGATTTTTTAACATAGGGTTAAGAATTTGATGTTCTGTTTAAACATTTAGCCCAGTTTTAAGAATGATGAATATTTGGATGGCTTTGGTTTATTATTATTGTTCCGAACGGTTTTCTCGCACAACTAAAATATTCTGAAGTTT

mRNA sequence

ATTAGAAGAAGCCAGAATTTGAAAAATGGAATCTTAGTAGTTATCGTTATTTCCACCATTTTGATTATTAAAGTGTCATTTAGTCCCCTGTCTCTCTCTACGCTCTCTGTTACTCTAATCTGTTCTTACTCTTTCAAAACTTTTCTCATTGTCCTCTACGTTTCTTCCCTCTCAATCCGCCCCCACTTTCCCCCAATCAATCACTCTCGCTTTTCAACTTGCTCTCTTCCGATTGAATTCCAGATCTTTCTGCGCTCTTCATCGATTTCCAACTTCATCAGGCTTTCCTGCAGATTTTTGGATCGGTGCTTGTGTTCGTATGGAAAATCCGGAATTGAAGAAGCTGAAACCATTTTGGCCTGAGGCTGACATTCAGAACAGATCTAAATGGCTATCAAATAGTCTTGAAGATGAATCGGAGTGTTGAGCAGATGCTGAAGGTGATCAAAGAAAATAGTGATAATTTCCCGAAGAGTGCTGATATGGACTCTCAAGTTGAGGAGTTCTCACGCTTGTATCAATCATTGGTTGAAAATGTGTTATCGCCGGAGCTACAATTGCAGGTGCCCGTATATTCTGATTGTGGATCTCCTCAAGGCACTCCGGAATTAAGTTCGGACCAAAAACAGGGTTTCAATTTGTCAAGCAATAGAGGCTTGGATATCTCTTTTGATTCTGGTGGTGGTAGCTCCAGTCTTTCTCTGAAGGATGGAACTGAATCTTCTTCTTCTTCTTCTTCTTCAGACTCGGAGTCAGAATCTTTCAACTCATCTGTCGACAATAACTATGTGGTTTCACGTGCTGAAAGAGATGGTCAAGGGCTGAAGAAAAAACTTCTTGGGATAGAAACTGGGCTTCCAAACATTAAAGGGGCGTTTTGGGTGGGTGAGGAAGACAAGGTGGATTATGATGAGCTACATGACAAACTCGCCAGGATTGAGGAAGAACTAAAAGTTTCGAATACAAAGCTTCAGTCATCTGAAAATGAGGTAGCTAGATTAAAGAGTGAGGTTGAGAAGAATGAAACAGCAATTTTGCTATCTGAAGGACTGCAGGCCCAGCTTGAGTCAGCTGAAAAAGATAAGCAAGAAATGGAGGCTAACCTTCAAGTGGAAAAAAGGCGAGTTGTGGAATTGGAACAACAAATAGAACAATTGGAAACTCGGGTTTTACAATCTGATTCAAAAGTTGAGAGATTGATTGAAGAATTAGAAATAAGCAAAGAAATCCTCAAAAGTTCAGATGACAAGATCACAAGGCTTACTCATGAACTCGAGATTACAAAATCTGACCACCATCTACAGATCAAGGAATTAGAAACTGCTTTTCAAGTTTCACAGGAGAGATTTCATGCTGAGAAAGAACAGATGCAGACTGACATTCTCAGACAAGTTGAAGCTGAGAAAACCGAAACGACAGCTCTCCACAACTCTCATTTGACAATTTTGCAAGGAGAGATCAGTCAGTTGAAGGAAGAACTTTCTGTAAGAAGCGAAAGTTTAGCAGCTTTGAATAGAAACCACGACGAACTAAAACTAAAATATGATATGCTAATGGCTGAGAAAGATGAAAGGAATGCCATGGTTCACTCACTTTTAGCAGATAAAGAATCCAGAGAATCTCATGTCCATGAATTGGAGGATCACCTGCAACATCTACAAGTAGAGAAGAAGAGCTTGATTGAAGGATCTGAAAGGCAGAACAAACAAATTGATGACTTGAAATTGAGACTCTCAGAATTGGAAGAAGAAGTGAACATGCAAAAGGGTATGATAGAGGATGGAGCTGAGGGGAAAAGGGAGGCCATAAGGCAGCTCTGTTTCTCTTTGGAGCATTACAGGAGTGGCTATCAAGAGCTTCGTGAAGCATTCATTGGGCACAAGCCGCGGCCGGTACTCTCTACATAGGTCTGTTTGCTTGTAAAATCTCTTAAGCTTATTACCATGTCTGTTTTTGTGCATTTTTATGAGTATTATGAGCCATTATTTAGTTGATTTTTTAACATAGGGTTAAGAATTTGATGTTCTGTTTAAACATTTAGCCCAGTTTTAAGAATGATGAATATTTGGATGGCTTTGGTTTATTATTATTGTTCCGAACGGTTTTCTCGCACAACTAAAATATTCTGAAGTTT

Coding sequence (CDS)

ATGAATCGGAGTGTTGAGCAGATGCTGAAGGTGATCAAAGAAAATAGTGATAATTTCCCGAAGAGTGCTGATATGGACTCTCAAGTTGAGGAGTTCTCACGCTTGTATCAATCATTGGTTGAAAATGTGTTATCGCCGGAGCTACAATTGCAGGTGCCCGTATATTCTGATTGTGGATCTCCTCAAGGCACTCCGGAATTAAGTTCGGACCAAAAACAGGGTTTCAATTTGTCAAGCAATAGAGGCTTGGATATCTCTTTTGATTCTGGTGGTGGTAGCTCCAGTCTTTCTCTGAAGGATGGAACTGAATCTTCTTCTTCTTCTTCTTCTTCAGACTCGGAGTCAGAATCTTTCAACTCATCTGTCGACAATAACTATGTGGTTTCACGTGCTGAAAGAGATGGTCAAGGGCTGAAGAAAAAACTTCTTGGGATAGAAACTGGGCTTCCAAACATTAAAGGGGCGTTTTGGGTGGGTGAGGAAGACAAGGTGGATTATGATGAGCTACATGACAAACTCGCCAGGATTGAGGAAGAACTAAAAGTTTCGAATACAAAGCTTCAGTCATCTGAAAATGAGGTAGCTAGATTAAAGAGTGAGGTTGAGAAGAATGAAACAGCAATTTTGCTATCTGAAGGACTGCAGGCCCAGCTTGAGTCAGCTGAAAAAGATAAGCAAGAAATGGAGGCTAACCTTCAAGTGGAAAAAAGGCGAGTTGTGGAATTGGAACAACAAATAGAACAATTGGAAACTCGGGTTTTACAATCTGATTCAAAAGTTGAGAGATTGATTGAAGAATTAGAAATAAGCAAAGAAATCCTCAAAAGTTCAGATGACAAGATCACAAGGCTTACTCATGAACTCGAGATTACAAAATCTGACCACCATCTACAGATCAAGGAATTAGAAACTGCTTTTCAAGTTTCACAGGAGAGATTTCATGCTGAGAAAGAACAGATGCAGACTGACATTCTCAGACAAGTTGAAGCTGAGAAAACCGAAACGACAGCTCTCCACAACTCTCATTTGACAATTTTGCAAGGAGAGATCAGTCAGTTGAAGGAAGAACTTTCTGTAAGAAGCGAAAGTTTAGCAGCTTTGAATAGAAACCACGACGAACTAAAACTAAAATATGATATGCTAATGGCTGAGAAAGATGAAAGGAATGCCATGGTTCACTCACTTTTAGCAGATAAAGAATCCAGAGAATCTCATGTCCATGAATTGGAGGATCACCTGCAACATCTACAAGTAGAGAAGAAGAGCTTGATTGAAGGATCTGAAAGGCAGAACAAACAAATTGATGACTTGAAATTGAGACTCTCAGAATTGGAAGAAGAAGTGAACATGCAAAAGGGTATGATAGAGGATGGAGCTGAGGGGAAAAGGGAGGCCATAAGGCAGCTCTGTTTCTCTTTGGAGCATTACAGGAGTGGCTATCAAGAGCTTCGTGAAGCATTCATTGGGCACAAGCCGCGGCCGGTACTCTCTACATAG

Protein sequence

MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNSSVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEELKVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVRSESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKKSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLST
Homology
BLAST of Lsi03G002510 vs. ExPASy Swiss-Prot
Match: F4KEW8 (Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.6e-25
Identity = 169/541 (31.24%), Postives = 271/541 (50.09%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQ--------VEEFSRLYQSLV---ENVL----- 60
           M+RSV++M+K+I+E++D+F K A+M  Q        V+EF R+Y++L    EN+      
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  -SP-ELQLQVPVYSDCGSPQGTPELSSDQ--KQGFNLSSNR--GLDISFDSGGGSSSLSL 120
            SP ELQ Q    SD  +   +   +S++  + G   S  R  G +    +GG  S L  
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KDGTESSSSSSSSDSESESFNSSVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWV 180
           KDG +   S+S +DSE ES +SSV N         D Q L K+++ +E  L   K    +
Sbjct: 164 KDGDD---SASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRM 223

Query: 181 GEEDKVD-----------YDELHDKLARIEEELKVSNTKLQSSENEVARLKSEVEKNETA 240
             E   +           + +   KLA  E+ELK  N KLQ+SE+++  LKS++ +   +
Sbjct: 224 QLEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARYLPS 283

Query: 241 ILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVELEQQIEQLETRVLQS---DSKVERLI 300
            L  E  +    + E D + +   L++   R+ E E+Q   +   V +S   D+K++ L 
Sbjct: 284 GLDDEQSEGAASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQ 343

Query: 301 EELEISKEIL-----KSSDDK--ITRLTHELEITKS-----DHHLQIKELETAFQVSQER 360
           + LE +++       K+S DK  + +L   + + KS     DH  +I++L+TA   ++E+
Sbjct: 344 DMLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDH--EIRDLKTALSDAEEK 403

Query: 361 FHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEE-LSVRSESLAALNRNH 420
              EK Q++ DI + +E EK       +     L+  +  L++E   V +E +       
Sbjct: 404 IFPEKAQVKADIAKLLE-EKIH----RDDQFKELEANVRYLEDERRKVNNEKI----EEE 463

Query: 421 DELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKKSLIEGSERQN 480
           ++LK + ++L  EK E+   + +L     SR+  V ELE  +  L         GSE   
Sbjct: 464 EKLKSEIEVLTLEKVEKGRCIETL-----SRK--VSELESEISRL---------GSE--I 523

Query: 481 KQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHK 493
           K  DD   R  E+E+EV  Q+  +E+ AE KRE IRQLCFSL++ R  Y+ LR AF GH 
Sbjct: 524 KARDD---RTMEMEKEVEKQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSGHP 549

BLAST of Lsi03G002510 vs. ExPASy Swiss-Prot
Match: Q84VY2 (Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 6.0e-22
Identity = 146/524 (27.86%), Postives = 241/524 (45.99%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQ--------VEEFSRLYQSLVENVLSPELQLQV 60
           M+  V  MLK+I+E++D+F K A M  Q        VEEF R+Y++L E       +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSD 120
              S+  S Q + E+SS  K+                     S       E   SSS +D
Sbjct: 104 NHTSEIQS-QSSLEISSPTKE-------------------KLSRRQSSHKEEEDSSSLTD 163

Query: 121 SESESFNSSVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYD---EL 180
           S S+S +SS ++       E   + L +++  +E  L   K    + +++ VD D   +L
Sbjct: 164 SGSDSDHSSAND-------EDGDEALIRRMAELELELQETKQKLLL-QQESVDGDNNVDL 223

Query: 181 HDKLARIEEELKVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEME 240
             K+   E ELK +N K++  E+E+A LK++++ +  +    + L A+ +S + DK++ +
Sbjct: 224 LHKITTYEGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTEDHLGAEQKSVDLDKEDTK 283

Query: 241 ANLQVEKRRVVELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELE 300
            +    K  V+ LE+++   + ++   + +   L  ELEI     K++++K+  L HELE
Sbjct: 284 EDAVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIG----KAAEEKLKSLQHELE 343

Query: 301 ITKSDHHLQIKELETAFQ---VSQERFHAEKEQMQTDILRQVEAEKTETTALHNS---HL 360
           + + D    I +L    +     QER    K  +Q D   ++ A KT  +          
Sbjct: 344 LAQRDADTYINKLNAEKKEVLKLQERLAMVKTSLQ-DRDNEIRALKTAVSDAEQKIFPEK 403

Query: 361 TILQGEISQLKEELSVRSESLAALN-----------RNHDELKLKYDMLMAEKDERNAMV 420
             ++GE+S++ EE S   E L  L               ++L+   + +   +DE N + 
Sbjct: 404 AQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKISGMRDESNVLR 463

Query: 421 HSLLADKESRESHVHELEDHLQHLQVEKKSLIEGSERQNKQIDDLKLRLSELEEEVNMQK 480
             +      RE  + E E H++ L +E+                L+ R SEL EEV   +
Sbjct: 464 EEI----GKREEKIKETEKHMEELHMEQVR--------------LRRRSSELTEEVERTR 513

Query: 481 GMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVL 497
               + AE KREAIRQLC SL+HYR GY  L     GHK + V+
Sbjct: 524 VSASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of Lsi03G002510 vs. ExPASy Swiss-Prot
Match: Q5U4E6 (Golgin subfamily A member 4 OS=Rattus norvegicus OX=10116 GN=Golga4 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 1.6e-06
Identity = 96/345 (27.83%), Postives = 166/345 (48.12%), Query Frame = 0

Query: 152 IKGAFWVGEEDKVDYDELHDKLARIEEELKVSNTKLQSSENEVARLKSEVEKNETAILLS 211
           IK     GEE+++    L  +L+R+ +E  VS  K  S +      +++++K     L S
Sbjct: 488 IKAVERAGEEERL---RLQHELSRVRQE-AVSMAKKNSEQ------RADLQKLHAEQLAS 547

Query: 212 --EGLQAQLESAEKDKQE-MEANLQVEKRRVVELEQQIEQLETRVLQSDSKVERLIEELE 271
             + L  +LES E++ QE M   L+  +   ++L Q+ EQ E+  L          EELE
Sbjct: 548 KEQELSQKLESRERELQEQMRMALEKSRSEYLKLTQEKEQQESLAL----------EELE 607

Query: 272 ISKE-ILKSSDDKITRLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQ 331
           + K+ IL  S++K+  L  E E+    H  +I+ELET+ + S +    + E++   +  +
Sbjct: 608 LQKKAILTESENKLQGLRQEAEV----HRTRIRELETSLEKSLQESRTQSERLAVHLEAE 667

Query: 332 VEAEKTETTALHNSHLTILQGEISQLKEELSVRSESLAALNRNH----DELKLKYDMLMA 391
               KTE TAL   H T L+G     +++ S+ +E L  L++ H    +EL+ K+     
Sbjct: 668 KSKHKTELTALAEKHRTELEG---LQQQQHSLWTERLQNLSQQHQAAVEELREKHQQ--- 727

Query: 392 EKDERNAMVHSLLADKESR-ESHVHELE----DHLQHLQVEKKSLIEGSERQNKQIDDLK 451
           EKD       +LL +KES  ++H+ ++     + L   Q+E +S+        K  D L 
Sbjct: 728 EKD-------ALLKEKESLFQAHIQDMNEKTLEKLDKKQMELESVSSELSEALKARDQLA 787

Query: 452 LRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGYQEL 484
             LS L  + +  K  +E   + +R   ++   S+    SG QE+
Sbjct: 788 EELSVLRGDADQMKQALEAELQEQRRHHQREVDSI----SGQQEI 791

BLAST of Lsi03G002510 vs. ExPASy Swiss-Prot
Match: F4JZY1 (COP1-interactive protein 1 OS=Arabidopsis thaliana OX=3702 GN=CIP1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-04
Identity = 85/377 (22.55%), Postives = 172/377 (45.62%), Query Frame = 0

Query: 137  GLKKKLLGIETGLPNI-KGAFWVGEEDKVDYDE---LHDKLARIEEELKVSNTKLQSSEN 196
            GL +K+ G E  L  + K    + EE +   +E   +HDK+     E+      + + +N
Sbjct: 1213 GLSEKIKGRELELETLGKQRSELDEELRTKKEENVQMHDKINVASSEIMALTELINNLKN 1272

Query: 197  EVARLKSEVEKNETAILL------SEGLQAQLESAEKDKQEMEA---NLQVEKRRVVELE 256
            E+  L  +V+K+ET   L         L  Q+   +K   E EA    L+ E +++ EL 
Sbjct: 1273 ELDSL--QVQKSETEAELEREKQEKSELSNQITDVQKALVEQEAAYNTLEEEHKQINELF 1332

Query: 257  QQIEQLETRVLQSDSKVERLIEELEISKEILKSS------DDKITRLTHELEITKSDHHL 316
            ++ E    +V     + +RL+EE    KE+          ++ +  L +ELE+   +   
Sbjct: 1333 KETEATLNKVTVDYKEAQRLLEER--GKEVTSRDSTIGVHEETMESLRNELEMKGDEIET 1392

Query: 317  ---QIKELETAFQVSQERFHAEKEQM--QTDILRQVEAEKTETTALHNSHLTI----LQG 376
               +I  +E   ++S ++    ++ +  + +  R+ EA+  E  AL   +LT+     +G
Sbjct: 1393 LMEKISNIEVKLRLSNQKLRVTEQVLTEKEEAFRKEEAKHLEEQALLEKNLTMTHETYRG 1452

Query: 377  EISQLKEELSVRSESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHE 436
             I ++ +++++  +   +++    E + +Y+  + E  +      + + ++         
Sbjct: 1453 MIKEIADKVNITVDGFQSMSEKLTEKQGRYEKTVMEASKILWTATNWVIERNH------- 1512

Query: 437  LEDHLQHLQVEKKSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQ 486
                      EK+ + +  E+++++I  L  ++ E E+E  M K  +    E KREAIRQ
Sbjct: 1513 ----------EKEKMNKEIEKKDEEIKKLGGKVREDEKEKEMMKETLMGLGEEKREAIRQ 1568

BLAST of Lsi03G002510 vs. ExPASy Swiss-Prot
Match: Q8IUD2 (ELKS/Rab6-interacting/CAST family member 1 OS=Homo sapiens OX=9606 GN=ERC1 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 5.6e-04
Identity = 90/343 (26.24%), Postives = 157/343 (45.77%), Query Frame = 0

Query: 130 RAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEELKVSNTKLQS 189
           R +RD   L ++     TG P       V E  + ++  LH +  R  +EL +    L+ 
Sbjct: 237 RIQRDLNQLFQQDSSSRTGEP------CVAELTEENFQRLHAEHERQAKELFLLRKTLEE 296

Query: 190 SENEVARLKSEVE-KNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVELEQQIEQ 249
            E  +   K  +  ++E+   L E LQ++  SA+  +++ E       RR+ E E  +  
Sbjct: 297 MELRIETQKQTLNARDESIKKLLEMLQSKGLSAKATEEDHE-----RTRRLAEAEMHVHH 356

Query: 250 LETRVLQSDSKVERLIEELEISKE-ILKSSDDKITRLTHELEITK-SDHHLQIKELETAF 309
           LE+ + Q + +   L EE+    E    S+  K  +   E++ +K S     +++LE   
Sbjct: 357 LESLLEQKEKENSMLREEMHRRFENAPDSAKTKALQTVIEMKDSKISSMERGLRDLEEEI 416

Query: 310 QVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVRSESLAA 369
           Q+ +       E+ + + ++Q+E        ++ SH   ++ ++ QLKEELS +      
Sbjct: 417 QMLKSNGALSTEEREEE-MKQME--------VYRSHSKFMKNKVEQLKEELSSK------ 476

Query: 370 LNRNHDELKLKYDMLMAE----KDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKKS 429
                +ELK K   L AE    K E +     LLA +   E+  ++  D  QH++V K+S
Sbjct: 477 -EAQWEELKKKAAGLQAEIGQVKQELSRKDTELLALQTKLETLTNQFSDSKQHIEVLKES 536

Query: 430 LIEGSERQ---NKQIDDLKLRLSELEEEVNMQKGMIEDGAEGK 463
           L    +R      ++D L+LRL E E  +N +   I+D AE K
Sbjct: 537 LTAKEQRAAILQTEVDALRLRLEEKETMLNKKTKQIQDMAEEK 552

BLAST of Lsi03G002510 vs. ExPASy TrEMBL
Match: A0A1S3CAE2 (protein NETWORKED 4A OS=Cucumis melo OX=3656 GN=LOC103498608 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 9.6e-225
Identity = 439/498 (88.15%), Postives = 464/498 (93.17%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENSD+FPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKTIKENSDSFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS
Sbjct: 92  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 151

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEEL 180
           SVDNNYVVSRAERDGQGLKKKLL IE+ LPNIKG FWVGEE KV+YDELHDK+A++EEEL
Sbjct: 152 SVDNNYVVSRAERDGQGLKKKLLAIESELPNIKGGFWVGEEVKVNYDELHDKIAKMEEEL 211

Query: 181 KVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVV 240
           KVSN KLQSSENEV RLKSEVEKNETAILLSEGLQAQLESAEKDKQ MEA LQV+K+   
Sbjct: 212 KVSNAKLQSSENEVTRLKSEVEKNETAILLSEGLQAQLESAEKDKQVMEAELQVKKK--- 271

Query: 241 ELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIK 300
               QIEQLETR+L SDSK+ERLI+ELEISKE+L SS+DKITRLTHELEITKSDHH QIK
Sbjct: 272 ----QIEQLETRILLSDSKIERLIKELEISKEMLNSSNDKITRLTHELEITKSDHHKQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVR 360
           ELETAFQVSQERFHAEKEQ++TDILRQVEAEK ET ALH S L++ QGEISQLKEELSVR
Sbjct: 332 ELETAFQVSQERFHAEKEQLETDILRQVEAEKAETKALHYSQLSMYQGEISQLKEELSVR 391

Query: 361 SESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEK 420
           SE+LA LNRNHDELKLKYDM+MAEKDE +A+V SLLADKESRESHVHELE  L+HLQVEK
Sbjct: 392 SENLADLNRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVHELEGQLKHLQVEK 451

Query: 421 KSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGY 480
             LIEGSERQNK+IDDLKLRL+ELEEEV MQK MIEDGAEGKREAIRQLCFSLEHYR+GY
Sbjct: 452 TRLIEGSERQNKEIDDLKLRLAELEEEVKMQKSMIEDGAEGKREAIRQLCFSLEHYRNGY 511

Query: 481 QELREAFIGHKPRPVLST 499
           QELREAFI HKPRPVLST
Sbjct: 512 QELREAFIAHKPRPVLST 522

BLAST of Lsi03G002510 vs. ExPASy TrEMBL
Match: A0A0A0KXR2 (NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G645160 PE=4 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 5.3e-223
Identity = 435/497 (87.53%), Postives = 463/497 (93.16%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENSD+FP+S DMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 120 MNRSVEQMLKTIKENSDSFPESVDMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 179

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS
Sbjct: 180 PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 239

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEEL 180
           SVDNNYVVSRAERDGQGLKKKLL IE+ LPNIKG FWVGEE KV+YDELHDK+A+IEEEL
Sbjct: 240 SVDNNYVVSRAERDGQGLKKKLLAIESELPNIKGGFWVGEEVKVNYDELHDKIAKIEEEL 299

Query: 181 KVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVV 240
           KVSN KLQSSENEV RLKSEVEKNETAILLSEGLQAQLES EKDKQ ME  LQV+K++  
Sbjct: 300 KVSNAKLQSSENEVTRLKSEVEKNETAILLSEGLQAQLESVEKDKQVMETELQVKKKK-- 359

Query: 241 ELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIK 300
                IEQLETR+ QSDSK+ERLI++LEISKE+LKSS+DKITRLTHELE TKSDHH+QIK
Sbjct: 360 -----IEQLETRISQSDSKIERLIKDLEISKEMLKSSNDKITRLTHELENTKSDHHIQIK 419

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVR 360
           ELETAFQVSQERFHAEKEQM+TDILRQVEAEKTET ALHNS LT+ QGEISQLKEELSVR
Sbjct: 420 ELETAFQVSQERFHAEKEQMETDILRQVEAEKTETKALHNSQLTMYQGEISQLKEELSVR 479

Query: 361 SESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEK 420
           SESLA LNRNHDELKLKYDM+MAEKDE +A+V SLLADKESRESHV+EL+  L+HLQV+K
Sbjct: 480 SESLADLNRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVNELKGQLKHLQVDK 539

Query: 421 KSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGY 480
           + LIEGSERQNK+IDDLKLRLSEL EEV MQK MIEDGAEGKREAIRQLCFSLEHYR+GY
Sbjct: 540 RRLIEGSERQNKEIDDLKLRLSELGEEVKMQKTMIEDGAEGKREAIRQLCFSLEHYRNGY 599

Query: 481 QELREAFIGHKPRPVLS 498
           QELREAFI HKPRPVLS
Sbjct: 600 QELREAFIAHKPRPVLS 609

BLAST of Lsi03G002510 vs. ExPASy TrEMBL
Match: A0A5A7TD60 (Protein NETWORKED 4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001610 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 7.1e-220
Identity = 430/491 (87.58%), Postives = 456/491 (92.87%), Query Frame = 0

Query: 8   MLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPEL 67
           MLK IKENSD+FPKSADMDSQVEEFSRLYQSLVENVLSPELQL VPVYSDCGSPQGTPEL
Sbjct: 1   MLKTIKENSDSFPKSADMDSQVEEFSRLYQSLVENVLSPELQLPVPVYSDCGSPQGTPEL 60

Query: 68  SSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNSSVDNNYV 127
           SSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNSSVDNNYV
Sbjct: 61  SSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNSSVDNNYV 120

Query: 128 VSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEELKVSNTKL 187
           VSRAERDGQGLKKKLL IE+ LPNI+G FWVGEE KV+YDELHDK+A++EEELKVSN KL
Sbjct: 121 VSRAERDGQGLKKKLLAIESELPNIEGGFWVGEEVKVNYDELHDKIAKMEEELKVSNAKL 180

Query: 188 QSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVELEQQIE 247
           QSSENEV RLKSEVEKNETAILLSEGLQAQLESAEKDKQ MEA LQV+K+       QIE
Sbjct: 181 QSSENEVTRLKSEVEKNETAILLSEGLQAQLESAEKDKQVMEAELQVKKK-------QIE 240

Query: 248 QLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIKELETAFQ 307
           QLETR+L SDSK+ERLI+ELEISKE+L SS+DKITRLTHELEITKSDHH QIKELETAFQ
Sbjct: 241 QLETRILLSDSKIERLIKELEISKEMLNSSNDKITRLTHELEITKSDHHKQIKELETAFQ 300

Query: 308 VSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVRSESLAAL 367
           VSQERFHAEKEQ++TDILRQVEAEK ET ALH S L++ QGEISQLKEELSVRSE+LA L
Sbjct: 301 VSQERFHAEKEQLETDILRQVEAEKAETKALHYSQLSMYQGEISQLKEELSVRSENLADL 360

Query: 368 NRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKKSLIEGS 427
           NRNHDELKLKYDM+MAEKDE +A+V SLLADKESRESHVHELE  L+HLQVEK  LIEGS
Sbjct: 361 NRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVHELEGQLKHLQVEKTRLIEGS 420

Query: 428 ERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAF 487
           ERQNK+IDDLKLRL+ELEEEV MQK MIEDGAEGKREAIRQLCFSLEHYR+GYQELREAF
Sbjct: 421 ERQNKEIDDLKLRLAELEEEVKMQKSMIEDGAEGKREAIRQLCFSLEHYRNGYQELREAF 480

Query: 488 IGHKPRPVLST 499
           I HKPRPVLST
Sbjct: 481 IAHKPRPVLST 484

BLAST of Lsi03G002510 vs. ExPASy TrEMBL
Match: A0A6J1JXC2 (protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111489163 PE=4 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.8e-218
Identity = 428/497 (86.12%), Postives = 459/497 (92.35%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+NS+NFPKSADMDSQVEEFSRLYQSL+EN+LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNSNNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQ TPELSSDQKQGFNLS NRGLDIS DSGGGSSSLSLKDGT   SSSSSSDSESESF+S
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGT--GSSSSSSDSESESFSS 151

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEEL 180
           SVDNNYVVSRAERDGQG KKK LG+ET L NIKGAFWVGEEDK++YDELH+K+ARIEEEL
Sbjct: 152 SVDNNYVVSRAERDGQGRKKKFLGLETELSNIKGAFWVGEEDKLNYDELHEKIARIEEEL 211

Query: 181 KVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVV 240
           KVSN KLQSSENEVARLKSE+EKNETA+ LSEGLQAQLESAEKDKQEM A+LQVEKRRVV
Sbjct: 212 KVSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVEKRRVV 271

Query: 241 ELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIK 300
           ELEQQIEQLETRVLQSDSK+E LIEELE+S+EILKSSDDKI RLTHELEITKSDH LQIK
Sbjct: 272 ELEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVR 360
           ELE+AFQVSQE F+AEKEQMQ D+LRQVEA+KTE  ALHNSHLT LQGEISQLKEEL V+
Sbjct: 332 ELESAFQVSQEIFNAEKEQMQADVLRQVEADKTEIRALHNSHLTSLQGEISQLKEELFVK 391

Query: 361 SESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEK 420
           S+SL+ALN NHDELKLKY+MLMAEKDE  AMVH+L+ADKESRESHVHELE HLQHLQ E+
Sbjct: 392 SQSLSALNSNHDELKLKYNMLMAEKDEATAMVHTLVADKESRESHVHELESHLQHLQEEE 451

Query: 421 KSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGY 480
           + LI GSE QNKQIDDLKLRLSE++EEVN Q  MIEDGAE KREAIRQLCFSLEHYRSGY
Sbjct: 452 EGLIAGSEIQNKQIDDLKLRLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGY 511

Query: 481 QELREAFIGHKPRPVLS 498
           +ELREAFIGHKPR VLS
Sbjct: 512 KELREAFIGHKPRQVLS 526

BLAST of Lsi03G002510 vs. ExPASy TrEMBL
Match: A0A6J1FN40 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111445431 PE=4 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.4e-215
Identity = 424/497 (85.31%), Postives = 454/497 (91.35%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+N DNFPKSADMDSQVEEFSRLYQSL+EN+LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNCDNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQ TPELSSDQKQGFNLS NRGLDIS DSGGGSSSLSLKDGT   SSSSSSDSESESF+S
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGT--GSSSSSSDSESESFSS 151

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEEL 180
           SVDNNYVVSRAERDGQG KKK LG+ET L NI+GAFWVGEEDK++Y ELH+K+ARIEEEL
Sbjct: 152 SVDNNYVVSRAERDGQGPKKKFLGLETELSNIEGAFWVGEEDKLNYHELHEKIARIEEEL 211

Query: 181 KVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVV 240
           KVSN KLQSSENEVARLKSE+EKNETA+ LSEGLQAQLESAEKDKQEM A+LQV KRRVV
Sbjct: 212 KVSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVLKRRVV 271

Query: 241 ELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIK 300
           ELEQQIEQLETRVLQSDSK+E LIEELE+S+EILKSSDDKI RLTHELEITKSDH LQIK
Sbjct: 272 ELEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVR 360
           ELE+AFQVSQE F+AEKEQ+Q D+LRQVEA+KTE  ALHNSHLT LQGEISQLKEEL V+
Sbjct: 332 ELESAFQVSQELFNAEKEQVQADVLRQVEADKTEMRALHNSHLTSLQGEISQLKEELLVK 391

Query: 361 SESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEK 420
           S+SL+ALN NHDELKLKYDMLMAEKDE  AMVH+L+ADKE RESHVHELE HLQHLQ EK
Sbjct: 392 SQSLSALNSNHDELKLKYDMLMAEKDEATAMVHTLVADKEYRESHVHELESHLQHLQEEK 451

Query: 421 KSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGY 480
           + LI GSE QNKQIDDLKL LSE++EEVN Q  MIEDGAE KREAIRQLCFSLEHYRSGY
Sbjct: 452 EGLIAGSESQNKQIDDLKLGLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGY 511

Query: 481 QELREAFIGHKPRPVLS 498
           +ELREAFIGHKPR VLS
Sbjct: 512 KELREAFIGHKPRQVLS 526

BLAST of Lsi03G002510 vs. NCBI nr
Match: XP_038889573.1 (protein NETWORKED 4A-like [Benincasa hispida] >XP_038889574.1 protein NETWORKED 4A-like [Benincasa hispida])

HSP 1 Score: 792.0 bits (2044), Expect = 3.1e-225
Identity = 442/499 (88.58%), Postives = 464/499 (92.99%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKTIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQGTPELSSDQKQGFNL+SNRGLDISFDSGGGSSSLSLKDGTE  SS SSSDSESESFNS
Sbjct: 92  PQGTPELSSDQKQGFNLTSNRGLDISFDSGGGSSSLSLKDGTE--SSPSSSDSESESFNS 151

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEED--KVDYDELHDKLARIEE 180
           SVDNNYVVSR+ERD  GLKKKLLGIET LPN K  FWVGEED  KV+YDELHDK+A+IEE
Sbjct: 152 SVDNNYVVSRSERDSHGLKKKLLGIETELPNSKRGFWVGEEDNHKVNYDELHDKIAKIEE 211

Query: 181 ELKVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRR 240
           ELKVSN KLQSSENEV+RLKSE+EKNETAI LSEGLQAQLE AEKDKQEMEA+LQV+KR 
Sbjct: 212 ELKVSNAKLQSSENEVSRLKSELEKNETAIWLSEGLQAQLELAEKDKQEMEADLQVKKR- 271

Query: 241 VVELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQ 300
                 QIEQLETR LQSDSK+ERLI+ELE SKE+LKSS DK+TRLTHELEITKSDHHLQ
Sbjct: 272 ------QIEQLETRALQSDSKIERLIQELETSKEMLKSSSDKVTRLTHELEITKSDHHLQ 331

Query: 301 IKELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELS 360
           +KELETAFQVSQERFHAEKEQMQTDILRQVEAEKTET ALHNSHLT+LQGEISQLKEEL 
Sbjct: 332 VKELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETKALHNSHLTVLQGEISQLKEELY 391

Query: 361 VRSESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQV 420
           V+S+ LAALN+NHDELKLKYDMLMAEKDE +AMV+SLLADKESRESHVHELE  LQHLQV
Sbjct: 392 VKSQGLAALNQNHDELKLKYDMLMAEKDEASAMVNSLLADKESRESHVHELEGDLQHLQV 451

Query: 421 EKKSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRS 480
           EK +LIEGSER+NKQIDDLKLRL ELEEEVN QK MIEDGAEGKREAIRQLCFSLEHYRS
Sbjct: 452 EKMNLIEGSERKNKQIDDLKLRLLELEEEVNKQKTMIEDGAEGKREAIRQLCFSLEHYRS 511

Query: 481 GYQELREAFIGHKPRPVLS 498
           GYQELREAFIGHKPRPVLS
Sbjct: 512 GYQELREAFIGHKPRPVLS 521

BLAST of Lsi03G002510 vs. NCBI nr
Match: XP_008459490.1 (PREDICTED: protein NETWORKED 4A [Cucumis melo])

HSP 1 Score: 789.3 bits (2037), Expect = 2.0e-224
Identity = 439/498 (88.15%), Postives = 464/498 (93.17%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENSD+FPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKTIKENSDSFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS
Sbjct: 92  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 151

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEEL 180
           SVDNNYVVSRAERDGQGLKKKLL IE+ LPNIKG FWVGEE KV+YDELHDK+A++EEEL
Sbjct: 152 SVDNNYVVSRAERDGQGLKKKLLAIESELPNIKGGFWVGEEVKVNYDELHDKIAKMEEEL 211

Query: 181 KVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVV 240
           KVSN KLQSSENEV RLKSEVEKNETAILLSEGLQAQLESAEKDKQ MEA LQV+K+   
Sbjct: 212 KVSNAKLQSSENEVTRLKSEVEKNETAILLSEGLQAQLESAEKDKQVMEAELQVKKK--- 271

Query: 241 ELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIK 300
               QIEQLETR+L SDSK+ERLI+ELEISKE+L SS+DKITRLTHELEITKSDHH QIK
Sbjct: 272 ----QIEQLETRILLSDSKIERLIKELEISKEMLNSSNDKITRLTHELEITKSDHHKQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVR 360
           ELETAFQVSQERFHAEKEQ++TDILRQVEAEK ET ALH S L++ QGEISQLKEELSVR
Sbjct: 332 ELETAFQVSQERFHAEKEQLETDILRQVEAEKAETKALHYSQLSMYQGEISQLKEELSVR 391

Query: 361 SESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEK 420
           SE+LA LNRNHDELKLKYDM+MAEKDE +A+V SLLADKESRESHVHELE  L+HLQVEK
Sbjct: 392 SENLADLNRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVHELEGQLKHLQVEK 451

Query: 421 KSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGY 480
             LIEGSERQNK+IDDLKLRL+ELEEEV MQK MIEDGAEGKREAIRQLCFSLEHYR+GY
Sbjct: 452 TRLIEGSERQNKEIDDLKLRLAELEEEVKMQKSMIEDGAEGKREAIRQLCFSLEHYRNGY 511

Query: 481 QELREAFIGHKPRPVLST 499
           QELREAFI HKPRPVLST
Sbjct: 512 QELREAFIAHKPRPVLST 522

BLAST of Lsi03G002510 vs. NCBI nr
Match: XP_031741879.1 (protein NETWORKED 4A [Cucumis sativus] >KAE8648970.1 hypothetical protein Csa_008117 [Cucumis sativus])

HSP 1 Score: 783.5 bits (2022), Expect = 1.1e-222
Identity = 435/497 (87.53%), Postives = 463/497 (93.16%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IKENSD+FP+S DMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS
Sbjct: 32  MNRSVEQMLKTIKENSDSFPESVDMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 91

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS
Sbjct: 92  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 151

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEEL 180
           SVDNNYVVSRAERDGQGLKKKLL IE+ LPNIKG FWVGEE KV+YDELHDK+A+IEEEL
Sbjct: 152 SVDNNYVVSRAERDGQGLKKKLLAIESELPNIKGGFWVGEEVKVNYDELHDKIAKIEEEL 211

Query: 181 KVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVV 240
           KVSN KLQSSENEV RLKSEVEKNETAILLSEGLQAQLES EKDKQ ME  LQV+K++  
Sbjct: 212 KVSNAKLQSSENEVTRLKSEVEKNETAILLSEGLQAQLESVEKDKQVMETELQVKKKK-- 271

Query: 241 ELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIK 300
                IEQLETR+ QSDSK+ERLI++LEISKE+LKSS+DKITRLTHELE TKSDHH+QIK
Sbjct: 272 -----IEQLETRISQSDSKIERLIKDLEISKEMLKSSNDKITRLTHELENTKSDHHIQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVR 360
           ELETAFQVSQERFHAEKEQM+TDILRQVEAEKTET ALHNS LT+ QGEISQLKEELSVR
Sbjct: 332 ELETAFQVSQERFHAEKEQMETDILRQVEAEKTETKALHNSQLTMYQGEISQLKEELSVR 391

Query: 361 SESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEK 420
           SESLA LNRNHDELKLKYDM+MAEKDE +A+V SLLADKESRESHV+EL+  L+HLQV+K
Sbjct: 392 SESLADLNRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVNELKGQLKHLQVDK 451

Query: 421 KSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGY 480
           + LIEGSERQNK+IDDLKLRLSEL EEV MQK MIEDGAEGKREAIRQLCFSLEHYR+GY
Sbjct: 452 RRLIEGSERQNKEIDDLKLRLSELGEEVKMQKTMIEDGAEGKREAIRQLCFSLEHYRNGY 511

Query: 481 QELREAFIGHKPRPVLS 498
           QELREAFI HKPRPVLS
Sbjct: 512 QELREAFIAHKPRPVLS 521

BLAST of Lsi03G002510 vs. NCBI nr
Match: KAA0039365.1 (protein NETWORKED 4A [Cucumis melo var. makuwa] >TYK00548.1 protein NETWORKED 4A [Cucumis melo var. makuwa])

HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-219
Identity = 430/491 (87.58%), Postives = 456/491 (92.87%), Query Frame = 0

Query: 8   MLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPEL 67
           MLK IKENSD+FPKSADMDSQVEEFSRLYQSLVENVLSPELQL VPVYSDCGSPQGTPEL
Sbjct: 1   MLKTIKENSDSFPKSADMDSQVEEFSRLYQSLVENVLSPELQLPVPVYSDCGSPQGTPEL 60

Query: 68  SSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNSSVDNNYV 127
           SSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNSSVDNNYV
Sbjct: 61  SSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNSSVDNNYV 120

Query: 128 VSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEELKVSNTKL 187
           VSRAERDGQGLKKKLL IE+ LPNI+G FWVGEE KV+YDELHDK+A++EEELKVSN KL
Sbjct: 121 VSRAERDGQGLKKKLLAIESELPNIEGGFWVGEEVKVNYDELHDKIAKMEEELKVSNAKL 180

Query: 188 QSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVELEQQIE 247
           QSSENEV RLKSEVEKNETAILLSEGLQAQLESAEKDKQ MEA LQV+K+       QIE
Sbjct: 181 QSSENEVTRLKSEVEKNETAILLSEGLQAQLESAEKDKQVMEAELQVKKK-------QIE 240

Query: 248 QLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIKELETAFQ 307
           QLETR+L SDSK+ERLI+ELEISKE+L SS+DKITRLTHELEITKSDHH QIKELETAFQ
Sbjct: 241 QLETRILLSDSKIERLIKELEISKEMLNSSNDKITRLTHELEITKSDHHKQIKELETAFQ 300

Query: 308 VSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVRSESLAAL 367
           VSQERFHAEKEQ++TDILRQVEAEK ET ALH S L++ QGEISQLKEELSVRSE+LA L
Sbjct: 301 VSQERFHAEKEQLETDILRQVEAEKAETKALHYSQLSMYQGEISQLKEELSVRSENLADL 360

Query: 368 NRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKKSLIEGS 427
           NRNHDELKLKYDM+MAEKDE +A+V SLLADKESRESHVHELE  L+HLQVEK  LIEGS
Sbjct: 361 NRNHDELKLKYDMVMAEKDEASAVVLSLLADKESRESHVHELEGQLKHLQVEKTRLIEGS 420

Query: 428 ERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAF 487
           ERQNK+IDDLKLRL+ELEEEV MQK MIEDGAEGKREAIRQLCFSLEHYR+GYQELREAF
Sbjct: 421 ERQNKEIDDLKLRLAELEEEVKMQKSMIEDGAEGKREAIRQLCFSLEHYRNGYQELREAF 480

Query: 488 IGHKPRPVLST 499
           I HKPRPVLST
Sbjct: 481 IAHKPRPVLST 484

BLAST of Lsi03G002510 vs. NCBI nr
Match: XP_022993014.1 (protein NETWORKED 4A [Cucurbita maxima] >XP_022993015.1 protein NETWORKED 4A [Cucurbita maxima])

HSP 1 Score: 768.5 bits (1983), Expect = 3.6e-218
Identity = 428/497 (86.12%), Postives = 459/497 (92.35%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQVEEFSRLYQSLVENVLSPELQLQVPVYSDCGS 60
           MNRSVEQMLK IK+NS+NFPKSADMDSQVEEFSRLYQSL+EN+LSPELQ QVP YSDCGS
Sbjct: 32  MNRSVEQMLKTIKDNSNNFPKSADMDSQVEEFSRLYQSLLENLLSPELQPQVPFYSDCGS 91

Query: 61  PQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSDSESESFNS 120
           PQ TPELSSDQKQGFNLS NRGLDIS DSGGGSSSLSLKDGT   SSSSSSDSESESF+S
Sbjct: 92  PQCTPELSSDQKQGFNLSCNRGLDISLDSGGGSSSLSLKDGT--GSSSSSSDSESESFSS 151

Query: 121 SVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEEL 180
           SVDNNYVVSRAERDGQG KKK LG+ET L NIKGAFWVGEEDK++YDELH+K+ARIEEEL
Sbjct: 152 SVDNNYVVSRAERDGQGRKKKFLGLETELSNIKGAFWVGEEDKLNYDELHEKIARIEEEL 211

Query: 181 KVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVV 240
           KVSN KLQSSENEVARLKSE+EKNETA+ LSEGLQAQLESAEKDKQEM A+LQVEKRRVV
Sbjct: 212 KVSNAKLQSSENEVARLKSELEKNETAMSLSEGLQAQLESAEKDKQEMGADLQVEKRRVV 271

Query: 241 ELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIK 300
           ELEQQIEQLETRVLQSDSK+E LIEELE+S+EILKSSDDKI RLTHELEITKSDH LQIK
Sbjct: 272 ELEQQIEQLETRVLQSDSKIEILIEELEMSREILKSSDDKIARLTHELEITKSDHRLQIK 331

Query: 301 ELETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVR 360
           ELE+AFQVSQE F+AEKEQMQ D+LRQVEA+KTE  ALHNSHLT LQGEISQLKEEL V+
Sbjct: 332 ELESAFQVSQEIFNAEKEQMQADVLRQVEADKTEIRALHNSHLTSLQGEISQLKEELFVK 391

Query: 361 SESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEK 420
           S+SL+ALN NHDELKLKY+MLMAEKDE  AMVH+L+ADKESRESHVHELE HLQHLQ E+
Sbjct: 392 SQSLSALNSNHDELKLKYNMLMAEKDEATAMVHTLVADKESRESHVHELESHLQHLQEEE 451

Query: 421 KSLIEGSERQNKQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGY 480
           + LI GSE QNKQIDDLKLRLSE++EEVN Q  MIEDGAE KREAIRQLCFSLEHYRSGY
Sbjct: 452 EGLIAGSEIQNKQIDDLKLRLSEMQEEVNRQMNMIEDGAERKREAIRQLCFSLEHYRSGY 511

Query: 481 QELREAFIGHKPRPVLS 498
           +ELREAFIGHKPR VLS
Sbjct: 512 KELREAFIGHKPRQVLS 526

BLAST of Lsi03G002510 vs. TAIR 10
Match: AT5G05180.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 150.6 bits (379), Expect = 3.3e-36
Identity = 144/446 (32.29%), Postives = 221/446 (49.55%), Query Frame = 0

Query: 78  SSNRGLDISFDSGGGSSSLSLKDGT----------------ESSSSSSSSDSESESFNSS 137
           +SN  +DIS D  G S  ++ K  T                +S+SS S  DS++E+F SS
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPKSDTVASNFDQGSSDVSGNHDSNSSYSDLDSDTEAFYSS 74

Query: 138 VDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEELK 197
             N+++VS    D   L                      E ++ Y+EL  K  + EEEL+
Sbjct: 75  F-NHHLVSPGSMDSHDL--------------------SPEKQMSYEELMKKYVQCEEELR 134

Query: 198 VSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVE 257
            ++ KLQ  E E+ +LK E EK E+ +L  E L+ + E A+ +    +  ++ E++RV+E
Sbjct: 135 TTSLKLQEFEQEIEKLK-ETEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLE 194

Query: 258 LEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIKE 317
           +++Q+  LET +     K E L+ E E+S++ L  S  +I++L   L             
Sbjct: 195 VQRQVVDLETELSDLSFKFEHLVNEHEVSRDCLDVSFSEISKLREML------------- 254

Query: 318 LETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVRS 377
                   Q+ F  EK ++  D ++  EAEK E           LQ EIS LK +L+ R 
Sbjct: 255 -----CDCQQNFSIEKTKL-VDQIKHSEAEKMEMQRKE----VELQAEISALKTDLATRG 314

Query: 378 ESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKK 437
           E + ALN++ D+ KL+YDMLMAEKD   A V +L A+  SR+  + ++E+ L  L  ++ 
Sbjct: 315 EHIEALNKDFDKHKLRYDMLMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQT 374

Query: 438 SLIEGSERQNKQIDDLKLRLSELEEEV------------------------NMQKGMIED 484
            L+  S      +++LK  + ELE EV                         +Q+  I  
Sbjct: 375 ELVSESGNAKNTVEELKAVVKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQ 415

BLAST of Lsi03G002510 vs. TAIR 10
Match: AT5G05180.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 50151 Blast hits to 28081 proteins in 1838 species: Archae - 860; Bacteria - 6013; Metazoa - 25867; Fungi - 3836; Plants - 2236; Viruses - 90; Other Eukaryotes - 11249 (source: NCBI BLink). )

HSP 1 Score: 136.3 bits (342), Expect = 6.5e-32
Identity = 138/446 (30.94%), Postives = 215/446 (48.21%), Query Frame = 0

Query: 78  SSNRGLDISFDSGGGSSSLSLKDGT----------------ESSSSSSSSDSESESFNSS 137
           +SN  +DIS D  G S  ++ K  T                +S+SS S  DS++E+F SS
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPKSDTVASNFDQGSSDVSGNHDSNSSYSDLDSDTEAFYSS 74

Query: 138 VDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYDELHDKLARIEEELK 197
             N+++VS    D   L                      E ++ Y+EL  K  + EEEL+
Sbjct: 75  F-NHHLVSPGSMDSHDL--------------------SPEKQMSYEELMKKYVQCEEELR 134

Query: 198 VSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVE 257
            ++ KLQ  E E+ +LK E EK E+ +L  E L+ + E A+ +    +  ++ E++RV+E
Sbjct: 135 TTSLKLQEFEQEIEKLK-ETEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLE 194

Query: 258 LEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELEITKSDHHLQIKE 317
           +++Q+  LET +     K E L+ E E +  I K      T+L  +++            
Sbjct: 195 VQRQVVDLETELSDLSFKFEHLVNEHEQNFSIEK------TKLVDQIK------------ 254

Query: 318 LETAFQVSQERFHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEELSVRS 377
                       H+E E+M+   +++ E E              LQ EIS LK +L+ R 
Sbjct: 255 ------------HSEAEKME---MQRKEVE--------------LQAEISALKTDLATRG 314

Query: 378 ESLAALNRNHDELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKK 437
           E + ALN++ D+ KL+YDMLMAEKD   A V +L A+  SR+  + ++E+ L  L  ++ 
Sbjct: 315 EHIEALNKDFDKHKLRYDMLMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQT 374

Query: 438 SLIEGSERQNKQIDDLKLRLSELEEEV------------------------NMQKGMIED 484
            L+  S      +++LK  + ELE EV                         +Q+  I  
Sbjct: 375 ELVSESGNAKNTVEELKAVVKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQ 391

BLAST of Lsi03G002510 vs. TAIR 10
Match: AT5G58320.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 118.2 bits (295), Expect = 1.8e-26
Identity = 169/541 (31.24%), Postives = 271/541 (50.09%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQ--------VEEFSRLYQSLV---ENVL----- 60
           M+RSV++M+K+I+E++D+F K A+M  Q        V+EF R+Y++L    EN+      
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  -SP-ELQLQVPVYSDCGSPQGTPELSSDQ--KQGFNLSSNR--GLDISFDSGGGSSSLSL 120
            SP ELQ Q    SD  +   +   +S++  + G   S  R  G +    +GG  S L  
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KDGTESSSSSSSSDSESESFNSSVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWV 180
           KDG +   S+S +DSE ES +SSV N         D Q L K+++ +E  L   K    +
Sbjct: 164 KDGDD---SASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRM 223

Query: 181 GEEDKVD-----------YDELHDKLARIEEELKVSNTKLQSSENEVARLKSEVEKNETA 240
             E   +           + +   KLA  E+ELK  N KLQ+SE+++  LKS++ +   +
Sbjct: 224 QLEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARYLPS 283

Query: 241 ILLSEGLQAQLESAEKDKQEMEANLQVEKRRVVELEQQIEQLETRVLQS---DSKVERLI 300
            L  E  +    + E D + +   L++   R+ E E+Q   +   V +S   D+K++ L 
Sbjct: 284 GLDDEQSEGAASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQ 343

Query: 301 EELEISKEIL-----KSSDDK--ITRLTHELEITKS-----DHHLQIKELETAFQVSQER 360
           + LE +++       K+S DK  + +L   + + KS     DH  +I++L+TA   ++E+
Sbjct: 344 DMLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDH--EIRDLKTALSDAEEK 403

Query: 361 FHAEKEQMQTDILRQVEAEKTETTALHNSHLTILQGEISQLKEE-LSVRSESLAALNRNH 420
              EK Q++ DI + +E EK       +     L+  +  L++E   V +E +       
Sbjct: 404 IFPEKAQVKADIAKLLE-EKIH----RDDQFKELEANVRYLEDERRKVNNEKI----EEE 463

Query: 421 DELKLKYDMLMAEKDERNAMVHSLLADKESRESHVHELEDHLQHLQVEKKSLIEGSERQN 480
           ++LK + ++L  EK E+   + +L     SR+  V ELE  +  L         GSE   
Sbjct: 464 EKLKSEIEVLTLEKVEKGRCIETL-----SRK--VSELESEISRL---------GSE--I 523

Query: 481 KQIDDLKLRLSELEEEVNMQKGMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHK 493
           K  DD   R  E+E+EV  Q+  +E+ AE KRE IRQLCFSL++ R  Y+ LR AF GH 
Sbjct: 524 KARDD---RTMEMEKEVEKQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSGHP 549

BLAST of Lsi03G002510 vs. TAIR 10
Match: AT2G30500.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 107.1 bits (266), Expect = 4.2e-23
Identity = 146/524 (27.86%), Postives = 241/524 (45.99%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQ--------VEEFSRLYQSLVENVLSPELQLQV 60
           M+  V  MLK+I+E++D+F K A M  Q        VEEF R+Y++L E       +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSD 120
              S+  S Q + E+SS  K+                     S       E   SSS +D
Sbjct: 104 NHTSEIQS-QSSLEISSPTKE-------------------KLSRRQSSHKEEEDSSSLTD 163

Query: 121 SESESFNSSVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYD---EL 180
           S S+S +SS ++       E   + L +++  +E  L   K    + +++ VD D   +L
Sbjct: 164 SGSDSDHSSAND-------EDGDEALIRRMAELELELQETKQKLLL-QQESVDGDNNVDL 223

Query: 181 HDKLARIEEELKVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEME 240
             K+   E ELK +N K++  E+E+A LK++++ +  +    + L A+ +S + DK++ +
Sbjct: 224 LHKITTYEGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTEDHLGAEQKSVDLDKEDTK 283

Query: 241 ANLQVEKRRVVELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELE 300
            +    K  V+ LE+++   + ++   + +   L  ELEI     K++++K+  L HELE
Sbjct: 284 EDAVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIG----KAAEEKLKSLQHELE 343

Query: 301 ITKSDHHLQIKELETAFQ---VSQERFHAEKEQMQTDILRQVEAEKTETTALHNS---HL 360
           + + D    I +L    +     QER    K  +Q D   ++ A KT  +          
Sbjct: 344 LAQRDADTYINKLNAEKKEVLKLQERLAMVKTSLQ-DRDNEIRALKTAVSDAEQKIFPEK 403

Query: 361 TILQGEISQLKEELSVRSESLAALN-----------RNHDELKLKYDMLMAEKDERNAMV 420
             ++GE+S++ EE S   E L  L               ++L+   + +   +DE N + 
Sbjct: 404 AQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKISGMRDESNVLR 463

Query: 421 HSLLADKESRESHVHELEDHLQHLQVEKKSLIEGSERQNKQIDDLKLRLSELEEEVNMQK 480
             +      RE  + E E H++ L +E+                L+ R SEL EEV   +
Sbjct: 464 EEI----GKREEKIKETEKHMEELHMEQVR--------------LRRRSSELTEEVERTR 513

Query: 481 GMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVL 497
               + AE KREAIRQLC SL+HYR GY  L     GHK + V+
Sbjct: 524 VSASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of Lsi03G002510 vs. TAIR 10
Match: AT2G30500.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 107.1 bits (266), Expect = 4.2e-23
Identity = 146/524 (27.86%), Postives = 241/524 (45.99%), Query Frame = 0

Query: 1   MNRSVEQMLKVIKENSDNFPKSADMDSQ--------VEEFSRLYQSLVENVLSPELQLQV 60
           M+  V  MLK+I+E++D+F K A M  Q        VEEF R+Y++L E       +LQ 
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  PVYSDCGSPQGTPELSSDQKQGFNLSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSSSD 120
              S+  S Q + E+SS  K+                     S       E   SSS +D
Sbjct: 104 NHTSEIQS-QSSLEISSPTKE-------------------KLSRRQSSHKEEEDSSSLTD 163

Query: 121 SESESFNSSVDNNYVVSRAERDGQGLKKKLLGIETGLPNIKGAFWVGEEDKVDYD---EL 180
           S S+S +SS ++       E   + L +++  +E  L   K    + +++ VD D   +L
Sbjct: 164 SGSDSDHSSAND-------EDGDEALIRRMAELELELQETKQKLLL-QQESVDGDNNVDL 223

Query: 181 HDKLARIEEELKVSNTKLQSSENEVARLKSEVEKNETAILLSEGLQAQLESAEKDKQEME 240
             K+   E ELK +N K++  E+E+A LK++++ +  +    + L A+ +S + DK++ +
Sbjct: 224 LHKITTYEGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTEDHLGAEQKSVDLDKEDTK 283

Query: 241 ANLQVEKRRVVELEQQIEQLETRVLQSDSKVERLIEELEISKEILKSSDDKITRLTHELE 300
            +    K  V+ LE+++   + ++   + +   L  ELEI     K++++K+  L HELE
Sbjct: 284 EDAVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIG----KAAEEKLKSLQHELE 343

Query: 301 ITKSDHHLQIKELETAFQ---VSQERFHAEKEQMQTDILRQVEAEKTETTALHNS---HL 360
           + + D    I +L    +     QER    K  +Q D   ++ A KT  +          
Sbjct: 344 LAQRDADTYINKLNAEKKEVLKLQERLAMVKTSLQ-DRDNEIRALKTAVSDAEQKIFPEK 403

Query: 361 TILQGEISQLKEELSVRSESLAALN-----------RNHDELKLKYDMLMAEKDERNAMV 420
             ++GE+S++ EE S   E L  L               ++L+   + +   +DE N + 
Sbjct: 404 AQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKISGMRDESNVLR 463

Query: 421 HSLLADKESRESHVHELEDHLQHLQVEKKSLIEGSERQNKQIDDLKLRLSELEEEVNMQK 480
             +      RE  + E E H++ L +E+                L+ R SEL EEV   +
Sbjct: 464 EEI----GKREEKIKETEKHMEELHMEQVR--------------LRRRSSELTEEVERTR 513

Query: 481 GMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVL 497
               + AE KREAIRQLC SL+HYR GY  L     GHK + V+
Sbjct: 524 VSASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KEW82.6e-2531.24Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1[more]
Q84VY26.0e-2227.86Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1[more]
Q5U4E61.6e-0627.83Golgin subfamily A member 4 OS=Rattus norvegicus OX=10116 GN=Golga4 PE=1 SV=2[more]
F4JZY11.5e-0422.55COP1-interactive protein 1 OS=Arabidopsis thaliana OX=3702 GN=CIP1 PE=1 SV=1[more]
Q8IUD25.6e-0426.24ELKS/Rab6-interacting/CAST family member 1 OS=Homo sapiens OX=9606 GN=ERC1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CAE29.6e-22588.15protein NETWORKED 4A OS=Cucumis melo OX=3656 GN=LOC103498608 PE=4 SV=1[more]
A0A0A0KXR25.3e-22387.53NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G645160 PE=4 SV... [more]
A0A5A7TD607.1e-22087.58Protein NETWORKED 4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169... [more]
A0A6J1JXC21.8e-21886.12protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111489163 PE=4 SV=1[more]
A0A6J1FN401.4e-21585.31protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111445431 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
XP_038889573.13.1e-22588.58protein NETWORKED 4A-like [Benincasa hispida] >XP_038889574.1 protein NETWORKED ... [more]
XP_008459490.12.0e-22488.15PREDICTED: protein NETWORKED 4A [Cucumis melo][more]
XP_031741879.11.1e-22287.53protein NETWORKED 4A [Cucumis sativus] >KAE8648970.1 hypothetical protein Csa_00... [more]
KAA0039365.11.5e-21987.58protein NETWORKED 4A [Cucumis melo var. makuwa] >TYK00548.1 protein NETWORKED 4A... [more]
XP_022993014.13.6e-21886.12protein NETWORKED 4A [Cucurbita maxima] >XP_022993015.1 protein NETWORKED 4A [Cu... [more]
Match NameE-valueIdentityDescription
AT5G05180.13.3e-3632.29unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G05180.26.5e-3230.94unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G58320.21.8e-2631.24Kinase interacting (KIP1-like) family protein [more]
AT2G30500.14.2e-2327.86Kinase interacting (KIP1-like) family protein [more]
AT2G30500.24.2e-2327.86Kinase interacting (KIP1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 427..454
NoneNo IPR availableCOILSCoilCoilcoord: 350..384
NoneNo IPR availableCOILSCoilCoilcoord: 166..207
NoneNo IPR availableCOILSCoilCoilcoord: 211..266
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..75
NoneNo IPR availablePANTHERPTHR32258:SF14TROPOMYOSINcoord: 2..323
NoneNo IPR availablePANTHERPTHR32258:SF14TROPOMYOSINcoord: 324..497
NoneNo IPR availablePANTHERPTHR32258PROTEIN NETWORKED 4Acoord: 324..497
NoneNo IPR availablePANTHERPTHR32258PROTEIN NETWORKED 4Acoord: 2..323
IPR011684Protein Networked (NET), actin-binding (NAB) domainPROSITEPS51774NABcoord: 1..62
score: 16.187939

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G002510.1Lsi03G002510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003779 actin binding