Lsi03G001240 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G001240
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	N-carbamoylputrescine amidase
Location	chr03: 1754574 .. 1756108 (+)
RNA-Seq Expression	Lsi03G001240
Synteny	Lsi03G001240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTAATGAGATTTTTAAGTAATGAAATGACCAATCTCTACCAAGCAACCAAAAGAGTATGCTTTGGTTGGGTCTCCCAAGGATCCATATTAGTACTTGCAAAGGCGGTTTCTTTAAGGTTAACCTTCATAAGCAAGCCTATGCTCAAACACTTTTAGAATGTGAAAAATATTTTACCCTACTCAAGTAAACTTAGGGGGAAGAGAAGGGTAAAGTTCGCGACACTCAGAAACTAGGTAGTGATCAGATGTATCCCTCTCCTTTTTCTCAACCCTGAATGGATAAATCCAATATTCTACTTAGGAGGTGAGAAATCTATCAATAAGTGAAAATATAGTTTGTTCCCATGTATTGAACCTAGTATGGGAACCATTTCAAAGGGTTTATTTATTTATTTATTTTTCTATGCTAAGGAAACGGAACTTTTCATTGATAAAATAAAAAGAGACTAATGCTCAAAGTACAAGGAAACCAAAATAAATTACTAAAAATACCAAAACAAATACAAGGAAAAGAGAAGGACTAAGCAGGAGGGAAAAAATCAATCCAGAACAAACGTGAACATTGGTAAAGGGGGTCTGGTTTATGGCTAGATCTTGTAAGGTTTCTCATAATTTTGGCTGTATGGTTTCCTAGGATTCTTCCATTTGGACATTGGTAAAGTAAAAAGAAACAACTATGTAACCCAAGTCGCAAATTTTAAAAACTGGGTTCTTGAATATTAATTTTACACATGCACATCAATTCCTTACATAATACTCTACATTCCTTTTTATATGTGATTCAGACTTAGATCCCAGGTTGAAGACTTTGTTCACTTTTTAAAAGAAATTTTTGCATTTTATTTTATAGATGTCAGCATTACATTCAAAACATAAGATATTTTAATGCTGCGTCCAGTCGGTGCTAAATAGTGAGGATGATTTGTTTCCATCTCTATACTTGACTGTACTTCTGTAATCAGAACTTTATGGTCTAATTTAAAGATGAGCAAAGTTGGTTGTTGGAGGTAACATGTAGTTTTCTAGAAGTTAAGAAGTGCATGCAATACTTGAGAAAATTTAATCAGCAATCTGCAATATTTAGAAGGTAAATGTGCAAAATCATTTTTATTTTAGCAATTTGCTGGGATCAGTGGTTTCCTGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCGGAGATATTGTTTTATCCTACAGCTATTGGTTCTGAACCTCAAGATGAAGGACTTGACTCTTGTGATCACTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGGTAAGTTTCTAGATGACAATATAGCTATATACATGAGGTTAGCTTAAGAATTAGTTTCTTTTTTTAGTTATCTGCTTTGTTTTGAGGGCCTTGAATGAGTTGTCTTTTCTGGGACCCTCCTTGGCCAACTAATGAACAATCATTGATACAAGTTGAATATTGATTTTGTGTGCGTATCTAAAGTTTTTGCTTTTAAATGTACTGTTTGGTGCTTTTGTTGGATATGATAACTTACCTCAGGCATGA

mRNA sequence

ATGGTTTGGTTTCCTGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCGGAGATATTGTTTTATCCTACAGCTATTGGTTCTGAACCTCAAGATGAAGGACTTGACTCTTGTGATCACTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGTTTTTGCTTTTAAATGTACTGTTTGGTGCTTTTGTTGGATATGATAACTTACCTCAGGCATGA

Coding sequence (CDS)

ATGGTTTGGTTTCCTGAGGCAGCCAGAGCCATGGTTCTTCAAGGTGCGGAGATATTGTTTTATCCTACAGCTATTGGTTCTGAACCTCAAGATGAAGGACTTGACTCTTGTGATCACTGGAAACGAGTAATGCAAGGGCATGCTGGTGCTAATGTGTTTTTGCTTTTAAATGTACTGTTTGGTGCTTTTGTTGGATATGATAACTTACCTCAGGCATGA

Protein sequence

MVWFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLLNVLFGAFVGYDNLPQA

Homology

[Close]

BLAST of Lsi03G001240 vs. ExPASy Swiss-Prot
Match: Q8VYF5 (N-carbamoylputrescine amidase OS=Arabidopsis thaliana OX=3702 GN=CPA PE=1 SV=2)

HSP 1 Score: 105.5 bits (262), Expect = 2.5e-22
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQD+GLDS DHW+RVMQGHAGANV  L+
Sbjct: 163 WFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 216

[Close]

BLAST of Lsi03G001240 vs. ExPASy Swiss-Prot
Match: Q9XGI9 (N-carbamoylputrescine amidase OS=Solanum lycopersicum OX=4081 GN=CPA PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 9.5e-22
Identity = 46/54 (85.19%), Postives = 50/54 (92.59%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAM LQGAE+LFYPTAIGSEPQD+GLDS DHW+RVMQGHAGANV  L+
Sbjct: 161 WFPEAARAMALQGAEVLFYPTAIGSEPQDDGLDSRDHWRRVMQGHAGANVVPLV 214

[Close]

BLAST of Lsi03G001240 vs. ExPASy Swiss-Prot
Match: Q3HVN1 (N-carbamoylputrescine amidase OS=Solanum tuberosum OX=4113 GN=CPA PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 9.5e-22
Identity = 46/54 (85.19%), Postives = 50/54 (92.59%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAM LQGAE+LFYPTAIGSEPQD+GLDS DHW+RVMQGHAGANV  L+
Sbjct: 161 WFPEAARAMALQGAEVLFYPTAIGSEPQDDGLDSRDHWRRVMQGHAGANVVPLV 214

[Close]

BLAST of Lsi03G001240 vs. ExPASy Swiss-Prot
Match: Q93XI4 (N-carbamoylputrescine amidase OS=Oryza sativa subsp. japonica OX=39947 GN=CPA PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.8e-20
Identity = 45/54 (83.33%), Postives = 48/54 (88.89%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPE ARAMVLQGAEILFYPTAIGSEPQD  LDS +HWKRVMQGHAGAN+  L+
Sbjct: 164 WFPECARAMVLQGAEILFYPTAIGSEPQDNNLDSREHWKRVMQGHAGANLVPLV 217

[Close]

BLAST of Lsi03G001240 vs. ExPASy TrEMBL
Match: A0A6P5ZJG1 (N-carbamoylputrescine amidase-like isoform X1 OS=Durio zibethinus OX=66656 GN=LOC111301265 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.8e-22
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV  L+
Sbjct: 161 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVVPLI 214

[Close]

BLAST of Lsi03G001240 vs. ExPASy TrEMBL
Match: A0A6P5ZJL2 (N-carbamoylputrescine amidase-like isoform X2 OS=Durio zibethinus OX=66656 GN=LOC111301265 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 7.6e-22
Identity = 50/50 (100.00%), Postives = 50/50 (100.00%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV 53
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV
Sbjct: 161 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV 210

[Close]

BLAST of Lsi03G001240 vs. ExPASy TrEMBL
Match: W1PSJ6 (CN hydrolase domain-containing protein OS=Amborella trichopoda OX=13333 GN=AMTR_s00040p00090100 PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 9.9e-22
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSC+HWKRVMQGHAGANV  LL
Sbjct: 159 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCEHWKRVMQGHAGANVVPLL 212

[Close]

BLAST of Lsi03G001240 vs. ExPASy TrEMBL
Match: A0A1S3CE56 (N-carbamoylputrescine amidase OS=Cucumis melo OX=3656 GN=LOC103499840 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.7e-21
Identity = 49/50 (98.00%), Postives = 50/50 (100.00%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV 53
           WFPEAARAMVLQGAEILFYPTAIGSEPQD+GLDSCDHWKRVMQGHAGANV
Sbjct: 161 WFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSCDHWKRVMQGHAGANV 210

[Close]

BLAST of Lsi03G001240 vs. ExPASy TrEMBL
Match: A0A1R3K5V9 (Carbon-nitrogen hydrolase OS=Corchorus capsularis OX=210143 GN=CCACVL1_02794 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.7e-21
Identity = 50/54 (92.59%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHW+RVMQGHAGANV  L+
Sbjct: 161 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWRRVMQGHAGANVVPLV 214

[Close]

BLAST of Lsi03G001240 vs. NCBI nr
Match: XP_022752545.1 (N-carbamoylputrescine amidase-like isoform X1 [Durio zibethinus])

HSP 1 Score: 112.8 bits (281), Expect = 1.2e-21
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV  L+
Sbjct: 161 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVVPLI 214

[Close]

BLAST of Lsi03G001240 vs. NCBI nr
Match: XP_022752546.1 (N-carbamoylputrescine amidase-like isoform X2 [Durio zibethinus])

HSP 1 Score: 112.5 bits (280), Expect = 1.6e-21
Identity = 50/50 (100.00%), Postives = 50/50 (100.00%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV 53
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV
Sbjct: 161 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV 210

[Close]

BLAST of Lsi03G001240 vs. NCBI nr
Match: KAF8395406.1 (hypothetical protein HHK36_019352 [Tetracentron sinense])

HSP 1 Score: 112.5 bits (280), Expect = 1.6e-21
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANV  L+
Sbjct: 208 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVVPLV 261

[Close]

BLAST of Lsi03G001240 vs. NCBI nr
Match: XP_006851426.1 (N-carbamoylputrescine amidase [Amborella trichopoda] >XP_020527402.1 N-carbamoylputrescine amidase [Amborella trichopoda] >ERN13007.1 hypothetical protein AMTR_s00040p00090100 [Amborella trichopoda])

HSP 1 Score: 112.1 bits (279), Expect = 2.0e-21
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSC+HWKRVMQGHAGANV  LL
Sbjct: 159 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCEHWKRVMQGHAGANVVPLL 212

[Close]

BLAST of Lsi03G001240 vs. NCBI nr
Match: KAF8403319.1 (hypothetical protein HHK36_011421 [Tetracentron sinense])

HSP 1 Score: 111.7 bits (278), Expect = 2.7e-21
Identity = 50/54 (92.59%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGAN+  L+
Sbjct: 143 WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANLVPLI 196

[Close]

BLAST of Lsi03G001240 vs. TAIR 10
Match: AT2G27450.1 (nitrilase-like protein 1 )

HSP 1 Score: 105.5 bits (262), Expect = 1.8e-23
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQD+GLDS DHW+RVMQGHAGANV  L+
Sbjct: 163 WFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 216

[Close]

BLAST of Lsi03G001240 vs. TAIR 10
Match: AT2G27450.2 (nitrilase-like protein 1 )

HSP 1 Score: 105.5 bits (262), Expect = 1.8e-23
Identity = 48/54 (88.89%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 3   WFPEAARAMVLQGAEILFYPTAIGSEPQDEGLDSCDHWKRVMQGHAGANVFLLL 57
           WFPEAARAMVLQGAEILFYPTAIGSEPQD+GLDS DHW+RVMQGHAGANV  L+
Sbjct: 190 WFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDHWRRVMQGHAGANVVPLV 243

The following BLAST results are available for this feature:

BLAST of Lsi03G001240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYF5	2.5e-22	88.89	N-carbamoylputrescine amidase OS=Arabidopsis thaliana OX=3702 GN=CPA PE=1 SV=2	[more]
Q9XGI9	9.5e-22	85.19	N-carbamoylputrescine amidase OS=Solanum lycopersicum OX=4081 GN=CPA PE=2 SV=1	[more]
Q3HVN1	9.5e-22	85.19	N-carbamoylputrescine amidase OS=Solanum tuberosum OX=4113 GN=CPA PE=2 SV=1	[more]
Q93XI4	1.8e-20	83.33	N-carbamoylputrescine amidase OS=Oryza sativa subsp. japonica OX=39947 GN=CPA PE...	[more]

BLAST of Lsi03G001240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6P5ZJG1	5.8e-22	94.44	N-carbamoylputrescine amidase-like isoform X1 OS=Durio zibethinus OX=66656 GN=LO...	[more]
A0A6P5ZJL2	7.6e-22	100.00	N-carbamoylputrescine amidase-like isoform X2 OS=Durio zibethinus OX=66656 GN=LO...	[more]
W1PSJ6	9.9e-22	94.44	CN hydrolase domain-containing protein OS=Amborella trichopoda OX=13333 GN=AMTR_...	[more]
A0A1S3CE56	1.7e-21	98.00	N-carbamoylputrescine amidase OS=Cucumis melo OX=3656 GN=LOC103499840 PE=4 SV=1	[more]
A0A1R3K5V9	1.7e-21	92.59	Carbon-nitrogen hydrolase OS=Corchorus capsularis OX=210143 GN=CCACVL1_02794 PE=...	[more]

BLAST of Lsi03G001240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022752545.1	1.2e-21	94.44	N-carbamoylputrescine amidase-like isoform X1 [Durio zibethinus]	[more]
XP_022752546.1	1.6e-21	100.00	N-carbamoylputrescine amidase-like isoform X2 [Durio zibethinus]	[more]
KAF8395406.1	1.6e-21	94.44	hypothetical protein HHK36_019352 [Tetracentron sinense]	[more]
XP_006851426.1	2.0e-21	94.44	N-carbamoylputrescine amidase [Amborella trichopoda] >XP_020527402.1 N-carbamoyl...	[more]
KAF8403319.1	2.7e-21	92.59	hypothetical protein HHK36_011421 [Tetracentron sinense]	[more]

BLAST of Lsi03G001240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27450.1	1.8e-23	88.89	nitrilase-like protein 1	[more]
AT2G27450.2	1.8e-23	88.89	nitrilase-like protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	3.60.110.10		coord: 2..68 e-value: 5.2E-12 score: 47.2
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 2..56
None	No IPR available	PANTHER	PTHR43674:SF6	NITRILASE C965.09-RELATED	coord: 3..57
None	No IPR available	PANTHER	PTHR43674	NITRILASE C965.09-RELATED	coord: 3..57
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 1..72 score: 13.055723

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G001240.1	Lsi03G001240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0033388	putrescine biosynthetic process from arginine
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0050126	N-carbamoylputrescine amidase activity