Lsi03G001140 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G001140
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerase III subunit RPC9
Location	chr03: 1623958 .. 1627702 (-)
RNA-Seq Expression	Lsi03G001140
Synteny	Lsi03G001140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATGTAAGTTCCTAATCCTCGTTCTTCCTGTAATTTGTTTCAATAATGTTCAAACTACTTGATTCTGCCATTTTTTTTAACAGAGCTCTCAATTTAAGAGCTCTCAATTTACTGTCGCTAACAGTTTATTGTTCGCAGATTAGAGGCCAATGCAGGCCCTCTTACAAATTTCGAGGTGCTCGATTTTTTAAGATCAAAGGGCGCAGCTAAGGATCCCACTAGGGTTCTTGCGAAAGTGGCACCATCTGAGTACAAGGTTATATATTTTCTTCCCTTCTTTTGTGCTGGACTATAAGTTTGATGCTGCAGGTTCTGATCAGATCTTTTTTTAATTTCTGTTTTTAGGTTTATGATTATTTGAATCAAACTCCTGCTTGTAATCAAACGAGAGAAGCTATAATTGAGTTTGTTGACAAGTCCAAGAAGTATGATCTTGCGAAAGCTGAAATTCTCAATGCCATCAATTTGCGCCCGATTACCAATGTTGAGATATATACGGTAATTTCCCCCTTTCTTGGCATTAATTCTTCCCTTTAATTTTGGAATGAATTTTATGCTATGTTTTTGTGTATAATCTCTGTTCTAAACTCATCTCCCATTTTCCCATATGTGAATAAGGTCATTTCTTTTCATTGGTGCCTCATATTTTTGAAGAGTCTAAGTGTTAGGAGGTAAAGGAACTGTGAATGCCTTGGAAAGTTTGGTGGACAGAGGAGATCCATAATATTTCAGCTATTTTAATATGAGGCCTTCTAATTGCAGTTTTAATGTGTTGGAAGAGAATTTTGTAGTCTTTGTAGCTATTTACTTTTCTATTGACATTACAAAAACTTAGATTCTCTGGCTATGGAGCACATTTATCATCTTCATCAATTAATTTGTAGTCCTATAAAGACTCACTTTGGGGTTGTCGTAAGGATTCCTAAAAACAGCTTTGGCTTTCGACAAAATAGTGTAGTGAAGATGTTTTTCCTTTCAAAACGATGAACCCTCATGTATAGTTATTAGTATCTACTTTCAAAACGTGATCCAACCTTTTCAAGCTGCCCAAAAATTATACTAAAAAATAGTTCTGTAAATAAAAACAAAAAGTTCTTTCCCCTGTACTCAAAGTATTTGTTATGCCGAAGTGAATATAGCTCAACTAGCATATGGGTGTGTTAGCGACTACGAGATCTGTGGTTCGAATTCTTCTACCCCTATTGTACTTAAAAATACTCAAAGTATCTATACCACGTTGTATTATACTTTCTAGTCATGGCCTATTATGCATCCTACTAGACATTCACTTTTTAGTATCAAGTTTTCTACTTGAGCCTTGAACTTAAGAATTTATAAGATATCTCCAAACATTCCACAACTCATGTCAACTAATACTAGGCCATTCTTGGGGGGTAAAGTTGTTTTTCAAAGTAGCTCCCAAACAAATTCTTGTCTGGCTTTTACCATGGTTTTTTTTTCTCTGTTGGGAGCTTCAAACTTGGGATGCTTTGTTTAGGAATTTTCATGTAAGAATCTGATGGACGAAGAAAACGAAAACATTAATTTAGATTAGTGGTTAAATTGAGATGTAGATTAATTGGGGTCTATTCCTTTCGTTTCTAGTTTATAGAGGACTGTGAGGATCGCATGGGAGATGATGGAATGCAAGAGCTTGTAGAGATGGTGAAACAGGTGTTCCCTACTCCTCAAACTCAACCAACATCTGAAGTAGCCCCGAAGGAAGCTAAAGAGGCCGAAACCATGGAAGAAAAAGAAGGAGCTGCGAATGCCGATGAAGAACCCATGGAAACGACTTGAACCCTGTTCAAAACTTAGGTGAGAAATTTTGGCTGATAGCTAAATAGAAAAGACCAAAATGAAAAATGTCATAACTTTCAATATTCAAATTTATAGCTAACGGAGTTGGGAGTGCATATTTTATTATTTCTATTATGCCCCGGGTATATATAAATAGTTACAGTCGGCTCATACAATTGTAAAGTGGTTTTTATTTTAAAAAATATACCTCATCAATTTAGTTTAAAAAATGACTAACAACCCTCCTCGATTAATATATTTTTTTCATCCTAATTATGTCTTGGATATTTACATCAGCCCATTCAATTGTCAAGTGATTTTTGTTTTAGAAAAATATCTCCCAACAACTTAGATACTAAAACGAATAACAACCCTAATTTTGAATAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGTTAAATCAAAGTTGAAAAAACAACTGTGTAGACCTAGATTGAGGTTGAGGGAGACAAAGGTACTTGCTTACATCGGAAATATAACGTAACAAAGTTTTAGGAATCAAAGTTTTGAGTACAAACTGAGAGTGTTCAATGGTGTAAAAGGTTGATGTTTAAGGTGGAGAGTTTTAAAGCTGGAGATTAGTGGGGATTTCAAATTGATTAATTTAATAATTAATATTAAATTAATCTTACACTTGGATTATGAACTAGTTTGAAGAGTTTTTGAAGATTTCAAATTGATTAATTTAATAATTAATATTAAATTAATCCTGTACCGTTCAGACTGAGCACTACTTGCCTTGAGTTAAGGTTAGTCATGTTTTAGATTAAAATTTTTGGATGATCCCATATTGTAACTTGTGAGGATTGAGCTTAATGTTAATATATATTTATCTTCCAAGCATCTTTAGTTCTTGTTTTAAATCCTTAGCATATATGATTGATGAGGATTGGCAAATTGGTTATGAAAGCATGTTAATTGCTAAAGTCCTCACATAATTTGTAATTGTTGTGTGTTGATTGCTTTGTAGGCAAAAATCGATTTATGGGTGTGCCTCTTTCACGATGTTATCAGAGCCACGTAGTTGGCATGTTTATGAAGAACAAATCTGTCGATTAATTAGGTGTTACCGGTTAATTAGTCGTCACGATACTTCCCTAGATCTTAACACGAGTGTTTGGTTAATTTAGAATGCATTTCATTCTTTGTTAGTTGATACATAGTTTAATTAGGATGTATGTCTTAATTTATGATTTTGGATGCTTAATTCGATACTTTCACATTAAGTAGACCGTGTGCTTAAGAACGCTTGCAGGAGTTGTCATATAATCATTGTTACATTTGTGGATTCCACTTCCCTAAGCTAGAGCTTTTAAGTTAGTAGCTTTCAATGTTCCAGTCGCTTTATTTACTTATATTGCATTTCTTATTTCCTCC

mRNA sequence

ATGAAAATATTAGAGGCCAATGCAGGCCCTCTTACAAATTTCGAGGTGCTCGATTTTTTAAGATCAAAGGGCGCAGCTAAGGATCCCACTAGGGTTCTTGCGAAAGTGGCACCATCTGAGTACAAGGTTTATGATTATTTGAATCAAACTCCTGCTTGTAATCAAACGAGAGAAGCTATAATTGAGTTTGTTGACAAGTCCAAGAAGTATGATCTTGCGAAAGCTGAAATTCTCAATGCCATCAATTTGCGCCCGATTACCAATGTTGAGATATATACGTTTATAGAGGACTGTGAGGATCGCATGGGAGATGATGGAATGCAAGAGCTTGTAGAGATGGTGAAACAGGTGTTCCCTACTCCTCAAACTCAACCAACATCTGAAGTAGCCCCGAAGGAAGCTAAAGAGGCCGAAACCATGGAAGAAAAAGAAGGAGCTGCGAATGCCGATGAAGAACCCATGGAAACGACTTGAACCCTGTTCAAAACTTAGGCAAAAATCGATTTATGGGTGTGCCTCTTTCACGATGTTATCAGAGCCACGTAGTTGGCATGTTTATGAAGAACAAATCTGTCGATTAATTAGGTGTTACCGGTTAATTAGTCGTCACGATACTTCCCTAGATCTTAACACGAGTGTTTGGTTAATTTAGAATGCATTTCATTCTTTGTTAGTTGATACATAGTTTAATTAGGATGTATGTCTTAATTTATGATTTTGGATGCTTAATTCGATACTTTCACATTAAGTAGACCGTGTGCTTAAGAACGCTTGCAGGAGTTGTCATATAATCATTGTTACATTTGTGGATTCCACTTCCCTAAGCTAGAGCTTTTAAGTTAGTAGCTTTCAATGTTCCAGTCGCTTTATTTACTTATATTGCATTTCTTATTTCCTCC

Coding sequence (CDS)

ATGAAAATATTAGAGGCCAATGCAGGCCCTCTTACAAATTTCGAGGTGCTCGATTTTTTAAGATCAAAGGGCGCAGCTAAGGATCCCACTAGGGTTCTTGCGAAAGTGGCACCATCTGAGTACAAGGTTTATGATTATTTGAATCAAACTCCTGCTTGTAATCAAACGAGAGAAGCTATAATTGAGTTTGTTGACAAGTCCAAGAAGTATGATCTTGCGAAAGCTGAAATTCTCAATGCCATCAATTTGCGCCCGATTACCAATGTTGAGATATATACGTTTATAGAGGACTGTGAGGATCGCATGGGAGATGATGGAATGCAAGAGCTTGTAGAGATGGTGAAACAGGTGTTCCCTACTCCTCAAACTCAACCAACATCTGAAGTAGCCCCGAAGGAAGCTAAAGAGGCCGAAACCATGGAAGAAAAAGAAGGAGCTGCGAATGCCGATGAAGAACCCATGGAAACGACTTGA

Protein sequence

MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAIIEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPTPQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT

Homology

[Close]

BLAST of Lsi03G001140 vs. ExPASy Swiss-Prot
Match: Q8VHM6 (DNA-directed RNA polymerase III subunit RPC9 OS=Rattus norvegicus OX=10116 GN=Crcp PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-10
Identity = 45/152 (29.61%), Postives = 77/152 (50.66%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFL------RSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACN 60
           M++ +ANA  L+N+EV   L      R +      +     +    Y+   Y+++TP  N
Sbjct: 1   MEVKDANAALLSNYEVFQLLTDLKEQRKESGKNKHSAGQQNLNAITYETLKYISKTPCKN 60

Query: 61  QTREAIIEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMV 120
           Q+   + EF+   K + L KAE L  +N RP+T VEI   +E+ E+R+ ++ ++ L+  V
Sbjct: 61  QSPAIVQEFLTAMKSHKLTKAEKLQLLNHRPMTAVEIQLMVEESEERLTEEQIEALLHTV 120

Query: 121 KQVFPTPQTQPTSEVAPKEAKEAETMEEKEGA 147
             + P     P  E +   + +A  MEE+E A
Sbjct: 121 TSILP---AGPEDEQSKSTSNDA-AMEEEEPA 148

[Close]

BLAST of Lsi03G001140 vs. ExPASy Swiss-Prot
Match: A0JN61 (DNA-directed RNA polymerase III subunit RPC9 OS=Bos taurus OX=9913 GN=CRCP PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.8e-10
Identity = 40/145 (27.59%), Postives = 73/145 (50.34%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFL------RSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACN 60
           M++ +ANA  L+N+EV   L      R +      +     +    Y+   Y+++TP  +
Sbjct: 1   MEVKDANAALLSNYEVFQLLTDLKEQRKESGKNKHSSGQQNLNTITYETLKYISKTPCKH 60

Query: 61  QTREAIIEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMV 120
           Q+ E + EF+   K + L KAE L  +N RP+T VEI   +E+ E+R+ ++ ++ L+  V
Sbjct: 61  QSPEIVREFLTAMKSHKLTKAEKLQLLNHRPVTAVEIQLMVEESEERLTEEQIEALLHTV 120

Query: 121 KQVFPT-PQTQPTSEVAPKEAKEAE 139
             + P  P+ +     +   A + E
Sbjct: 121 TSILPAGPEAEQQQNASDDVAMDEE 145

[Close]

BLAST of Lsi03G001140 vs. ExPASy Swiss-Prot
Match: O35427 (DNA-directed RNA polymerase III subunit RPC9 OS=Mus musculus OX=10090 GN=Crcp PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 6.3e-10
Identity = 44/152 (28.95%), Postives = 73/152 (48.03%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFL------RSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACN 60
           M++ +ANA  L+N+EV   L      R +      +     +    Y+   Y+++TP  N
Sbjct: 1   MEVKDANAALLSNYEVFQLLTDLKEQRKESGKNKHSAGQQNLNAITYETLKYISKTPCRN 60

Query: 61  QTREAIIEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMV 120
           Q+   + EF+   K + L KAE L  +N RP+T VEI   +E+ E+R+ ++ ++ L+  V
Sbjct: 61  QSPAIVQEFLTAMKSHKLTKAEKLQLLNHRPMTAVEIQLMVEESEERLTEEQIEALLHTV 120

Query: 121 KQVFPTPQTQPTSEVAPKEAKEAETMEEKEGA 147
             + P       S    K       MEE+E A
Sbjct: 121 TSILPAGPEDEQS----KSTSNDVAMEEEEPA 148

[Close]

BLAST of Lsi03G001140 vs. ExPASy Swiss-Prot
Match: Q60482 (DNA-directed RNA polymerase III subunit RPC9 OS=Cavia porcellus OX=10141 GN=CRCP PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-09
Identity = 47/149 (31.54%), Postives = 77/149 (51.68%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPS---------EYKVYDYLNQTP 60
           M++ +AN+  L+N+EV   L      KD  R   K+  S          Y+   YL++TP
Sbjct: 1   MEVKDANSALLSNYEVFQLLTD---LKDQRRESGKMKHSAGQQNLNTITYETLKYLSKTP 60

Query: 61  ACNQTREAIIEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRM-GDDGMQEL 120
             +Q+ E + EF+   K + L KAE L  +N RP+T VEI   +E+ E+R   ++ ++ L
Sbjct: 61  CRHQSPEIVREFLTAMKSHKLTKAEKLQLLNHRPMTAVEIQLMVEETEERFTEEEQIEAL 120

Query: 121 VEMVKQVFPT-PQTQPTSEVAPKEAKEAE 139
           +  V  + P  P+ +   ++A  EA E E
Sbjct: 121 LHTVTHILPAEPEVE---QMASTEAMEEE 143

[Close]

BLAST of Lsi03G001140 vs. ExPASy Swiss-Prot
Match: O75575 (DNA-directed RNA polymerase III subunit RPC9 OS=Homo sapiens OX=9606 GN=CRCP PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-09
Identity = 42/152 (27.63%), Postives = 77/152 (50.66%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFL------RSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACN 60
           M++ +AN+  L+N+EV   L      R +      +     +    Y+   Y+++TP  +
Sbjct: 1   MEVKDANSALLSNYEVFQLLTDLKEQRKESGKNKHSSGQQNLNTITYETLKYISKTPCRH 60

Query: 61  QTREAIIEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMV 120
           Q+ E + EF+   K + L KAE L  +N RP+T VEI   +E+ E+R+ ++ ++ L+  V
Sbjct: 61  QSPEIVREFLTALKSHKLTKAEKLQLLNHRPVTAVEIQLMVEESEERLTEEQIEALLHTV 120

Query: 121 KQVFPTPQTQPTSEVAPKEAKEAETMEEKEGA 147
             + P    +P +E   K       M+E++ A
Sbjct: 121 TSILP---AEPEAE-QKKNTNSNVAMDEEDPA 148

[Close]

BLAST of Lsi03G001140 vs. ExPASy TrEMBL
Match: A0A6J1L324 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita maxima OX=3661 GN=LOC111499396 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 8.6e-63
Identity = 130/157 (82.80%), Postives = 140/157 (89.17%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAG LTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQT ACNQTREAI
Sbjct: 1   MKILEANAGALTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTHACNQTREAI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAE+LN +NLRPIT VEI+  IED + RMGDDG+QEL EMV+QVFPT
Sbjct: 61  IEFVDKSKKYDLAKAELLNILNLRPITPVEIFPIIEDSDVRMGDDGIQELTEMVEQVFPT 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           PQT+PTSEVAP E KEAETM E+    +A +EPMETT
Sbjct: 121 PQTEPTSEVAPMEGKEAETMAEQVEIVDAGQEPMETT 157

[Close]

BLAST of Lsi03G001140 vs. ExPASy TrEMBL
Match: A0A6J1H2X6 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LOC111459926 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-62
Identity = 129/157 (82.17%), Postives = 139/157 (88.54%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAG LTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQT ACNQTREAI
Sbjct: 1   MKILEANAGALTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTHACNQTREAI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAE+LN +NLRPIT VEI+  IED + RMGDDG+QEL EMV+QVFPT
Sbjct: 61  IEFVDKSKKYDLAKAELLNILNLRPITPVEIFPIIEDSDVRMGDDGIQELTEMVEQVFPT 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           PQT+PTSE  P E KEAETM E+E   +A +EPMETT
Sbjct: 121 PQTEPTSEAVPMEGKEAETMAEQEEIVDAGQEPMETT 157

[Close]

BLAST of Lsi03G001140 vs. ExPASy TrEMBL
Match: A0A6J1CCS4 (DNA-directed RNA polymerase III subunit RPC9 OS=Momordica charantia OX=3673 GN=LOC111009544 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 6.2e-61
Identity = 127/157 (80.89%), Postives = 134/157 (85.35%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKV PSEYKVYDYLNQTPAC Q RE+I
Sbjct: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVEPSEYKVYDYLNQTPACYQRRESI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAEILN INLRP T VEI+  IED + RMGDDG+QEL EMV+ VFP 
Sbjct: 61  IEFVDKSKKYDLAKAEILNIINLRPTTPVEIFPIIEDSDVRMGDDGIQELTEMVEGVFPP 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           PQTQPT +VAPKE KE E M E+EG    D EPMETT
Sbjct: 121 PQTQPTPDVAPKEGKEDEAMAEQEGGEGGDGEPMETT 157

[Close]

BLAST of Lsi03G001140 vs. ExPASy TrEMBL
Match: A0A6J1EQC1 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LOC111436740 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.2e-58
Identity = 126/157 (80.25%), Postives = 136/157 (86.62%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPA  QTREAI
Sbjct: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPASYQTREAI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAEILN +NLRPIT VEI+  IE+ + RMGDDG+QEL +MV+QVFP 
Sbjct: 61  IEFVDKSKKYDLAKAEILNIVNLRPITPVEIFPIIEESDVRMGDDGIQELTKMVEQVFPA 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
             +Q TS VAPKE KEAE ME++EG   ADEE M TT
Sbjct: 121 --SQSTSVVAPKECKEAEAMEKEEGVGGADEETMVTT 155

[Close]

BLAST of Lsi03G001140 vs. ExPASy TrEMBL
Match: A0A5A7UV88 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001320 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 8.4e-58
Identity = 120/160 (75.00%), Postives = 137/160 (85.62%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKV PSEYKV+DYLNQTPACNQTREA+
Sbjct: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVLPSEYKVFDYLNQTPACNQTREAV 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDG---MQELVEMVKQV 120
           I FV+KSK+YDLAKAEILN INLRPIT VE++  IE+C+ RMG++    M+E+  MV+ V
Sbjct: 61  IAFVEKSKEYDLAKAEILNIINLRPITPVELFPIIENCDVRMGEEATERMEEMGSMVEHV 120

Query: 121 FPTPQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           FPTPQ QPT E  P E  EAETMEE+EGA +A EEP++TT
Sbjct: 121 FPTPQIQPTFEATPNEGNEAETMEEQEGAGDAVEEPIQTT 160

[Close]

BLAST of Lsi03G001140 vs. NCBI nr
Match: XP_023548749.1 (uncharacterized protein LOC111807313 [Cucurbita pepo subsp. pepo] >KAG6574960.1 DNA-directed RNA polymerase III subunit RPC9, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013528.1 DNA-directed RNA polymerase III subunit RPC9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250.8 bits (639), Expect = 8.0e-63
Identity = 130/157 (82.80%), Postives = 140/157 (89.17%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAG LTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQT ACNQTREAI
Sbjct: 1   MKILEANAGALTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTHACNQTREAI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAE+LN +NLRPIT VEI+  IED + RMGDDG+QEL EMV+QVFPT
Sbjct: 61  IEFVDKSKKYDLAKAELLNILNLRPITPVEIFPIIEDSDVRMGDDGIQELTEMVEQVFPT 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           PQT+PTSE AP E KEAETM E+E   +A +EPMETT
Sbjct: 121 PQTEPTSEAAPMEGKEAETMAEQEEIVDAGQEPMETT 157

[Close]

BLAST of Lsi03G001140 vs. NCBI nr
Match: XP_023006759.1 (uncharacterized protein LOC111499396 [Cucurbita maxima] >XP_023006760.1 uncharacterized protein LOC111499396 [Cucurbita maxima])

HSP 1 Score: 249.6 bits (636), Expect = 1.8e-62
Identity = 130/157 (82.80%), Postives = 140/157 (89.17%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAG LTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQT ACNQTREAI
Sbjct: 1   MKILEANAGALTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTHACNQTREAI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAE+LN +NLRPIT VEI+  IED + RMGDDG+QEL EMV+QVFPT
Sbjct: 61  IEFVDKSKKYDLAKAELLNILNLRPITPVEIFPIIEDSDVRMGDDGIQELTEMVEQVFPT 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           PQT+PTSEVAP E KEAETM E+    +A +EPMETT
Sbjct: 121 PQTEPTSEVAPMEGKEAETMAEQVEIVDAGQEPMETT 157

[Close]

BLAST of Lsi03G001140 vs. NCBI nr
Match: XP_022958741.1 (uncharacterized protein LOC111459926 [Cucurbita moschata])

HSP 1 Score: 249.2 bits (635), Expect = 2.3e-62
Identity = 129/157 (82.17%), Postives = 139/157 (88.54%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAG LTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQT ACNQTREAI
Sbjct: 1   MKILEANAGALTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTHACNQTREAI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAE+LN +NLRPIT VEI+  IED + RMGDDG+QEL EMV+QVFPT
Sbjct: 61  IEFVDKSKKYDLAKAELLNILNLRPITPVEIFPIIEDSDVRMGDDGIQELTEMVEQVFPT 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           PQT+PTSE  P E KEAETM E+E   +A +EPMETT
Sbjct: 121 PQTEPTSEAVPMEGKEAETMAEQEEIVDAGQEPMETT 157

[Close]

BLAST of Lsi03G001140 vs. NCBI nr
Match: XP_038875324.1 (uncharacterized protein LOC120067805 [Benincasa hispida])

HSP 1 Score: 248.1 bits (632), Expect = 5.2e-62
Identity = 129/162 (79.63%), Postives = 142/162 (87.65%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQ+REAI
Sbjct: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQSREAI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDG-----MQELVEMVK 120
           +EFV++SK+YDLAKAEILN IN RPI  VE++  IE CE RMGDDG     MQELV MV+
Sbjct: 61  LEFVERSKEYDLAKAEILNIINCRPIAPVELFPIIESCEARMGDDGVDGGRMQELVTMVE 120

Query: 121 QVFPTPQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           QVFPTPQ +PTSEVAPKE KEAETMEE+E A + ++EPME T
Sbjct: 121 QVFPTPQNEPTSEVAPKEGKEAETMEEQEVAGDVNDEPMEKT 162

[Close]

BLAST of Lsi03G001140 vs. NCBI nr
Match: XP_022138348.1 (uncharacterized protein LOC111009544 [Momordica charantia])

HSP 1 Score: 243.4 bits (620), Expect = 1.3e-60
Identity = 127/157 (80.89%), Postives = 134/157 (85.35%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKV PSEYKVYDYLNQTPAC Q RE+I
Sbjct: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVEPSEYKVYDYLNQTPACYQRRESI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGDDGMQELVEMVKQVFPT 120
           IEFVDKSKKYDLAKAEILN INLRP T VEI+  IED + RMGDDG+QEL EMV+ VFP 
Sbjct: 61  IEFVDKSKKYDLAKAEILNIINLRPTTPVEIFPIIEDSDVRMGDDGIQELTEMVEGVFPP 120

Query: 121 PQTQPTSEVAPKEAKEAETMEEKEGAANADEEPMETT 158
           PQTQPT +VAPKE KE E M E+EG    D EPMETT
Sbjct: 121 PQTQPTPDVAPKEGKEDEAMAEQEGGEGGDGEPMETT 157

[Close]

BLAST of Lsi03G001140 vs. TAIR 10
Match: AT5G62950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-32
Identity = 79/140 (56.43%), Postives = 98/140 (70.00%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKI++ANAG LTNFEVLDFL S+GA+KD TRV+A +A SEYKVYDYL +T A  QTRE+I
Sbjct: 1   MKIVKANAGALTNFEVLDFLNSRGASKDTTRVIAPIARSEYKVYDYLVETAASTQTRESI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGD-DGMQELVEMVKQVFP 120
            +F DK K + LAKAEILN INLRP + VE+   IE+ +DR  D DG+ ELV+ +    P
Sbjct: 61  NKFADKCKDFKLAKAEILNIINLRPSSIVELLPIIENLDDREIDTDGILELVKDLLPPLP 120

Query: 121 TPQTQPTSEVAPKEAKEAET 140
           T         +PK+  E ET
Sbjct: 121 T-------TASPKDDDEEET 133

[Close]

BLAST of Lsi03G001140 vs. TAIR 10
Match: AT5G62950.3 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-32
Identity = 79/140 (56.43%), Postives = 98/140 (70.00%), Query Frame = 0


Query: 1   MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
           MKI++ANAG LTNFEVLDFL S+GA+KD TRV+A +A SEYKVYDYL +T A  QTRE+I
Sbjct: 1   MKIVKANAGALTNFEVLDFLNSRGASKDTTRVIAPIARSEYKVYDYLVETAASTQTRESI 60

Query: 61  IEFVDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGD-DGMQELVEMVKQVFP 120
            +F DK K + LAKAEILN INLRP + VE+   IE+ +DR  D DG+ ELV+ +    P
Sbjct: 61  NKFADKCKDFKLAKAEILNIINLRPSSIVELLPIIENLDDREIDTDGILELVKDLLPPLP 120

Query: 121 TPQTQPTSEVAPKEAKEAET 140
           T         +PK+  E ET
Sbjct: 121 T-------TASPKDDDEEET 133

[Close]

BLAST of Lsi03G001140 vs. TAIR 10
Match: AT3G28956.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.1e-28
Identity = 75/142 (52.82%), Postives = 98/142 (69.01%), Query Frame = 0


Query: 4   LEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAIIEF 63
           ++ANAG LTNFE+LDFL S+GA+KD TRV+A +A SEYKVYDYL +T A  QTRE++ + 
Sbjct: 1   MKANAGALTNFELLDFLNSRGASKDTTRVIAPIARSEYKVYDYLVETAASTQTRESVNKS 60

Query: 64  VDKSKKYDLAKAEILNAINLRPITNVEIYTFIEDCEDRMGD-DGMQELVEMVKQVFPTPQ 123
            DK K + LAKAEILN INL P + VE+   IE+ +DR  D DG   ++E+VK + P   
Sbjct: 61  ADKCKDFKLAKAEILNIINLWPSSIVELLPIIENLDDREIDTDG---ILELVKDLLP--- 120

Query: 124 TQPTSEVAPKEAKEAETMEEKE 145
             PT+E +PK+  E E  E  E
Sbjct: 121 PLPTAE-SPKDNDEEEETENGE 135

[Close]

BLAST of Lsi03G001140 vs. TAIR 10
Match: AT5G62950.2 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 122.9 bits (307), Expect = 2.3e-28
Identity = 62/91 (68.13%), Postives = 74/91 (81.32%), Query Frame = 0


Query: 1  MKILEANAGPLTNFEVLDFLRSKGAAKDPTRVLAKVAPSEYKVYDYLNQTPACNQTREAI 60
          MKI++ANAG LTNFEVLDFL S+GA+KD TRV+A +A SEYKVYDYL +T A  QTRE+I
Sbjct: 1  MKIVKANAGALTNFEVLDFLNSRGASKDTTRVIAPIARSEYKVYDYLVETAASTQTRESI 60

Query: 61 IEFVDKSKKYDLAKAEILNAINLRPITNVEI 92
           +F DK K + LAKAEILN INLRP + VE+
Sbjct: 61 NKFADKCKDFKLAKAEILNIINLRPSSIVEL 91

The following BLAST results are available for this feature:

BLAST of Lsi03G001140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VHM6	2.2e-10	29.61	DNA-directed RNA polymerase III subunit RPC9 OS=Rattus norvegicus OX=10116 GN=Cr...	[more]
A0JN61	2.8e-10	27.59	DNA-directed RNA polymerase III subunit RPC9 OS=Bos taurus OX=9913 GN=CRCP PE=2 ...	[more]
O35427	6.3e-10	28.95	DNA-directed RNA polymerase III subunit RPC9 OS=Mus musculus OX=10090 GN=Crcp PE...	[more]
Q60482	1.4e-09	31.54	DNA-directed RNA polymerase III subunit RPC9 OS=Cavia porcellus OX=10141 GN=CRCP...	[more]
O75575	1.8e-09	27.63	DNA-directed RNA polymerase III subunit RPC9 OS=Homo sapiens OX=9606 GN=CRCP PE=...	[more]

BLAST of Lsi03G001140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L324	8.6e-63	82.80	DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1H2X6	1.1e-62	82.17	DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CCS4	6.2e-61	80.89	DNA-directed RNA polymerase III subunit RPC9 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1EQC1	2.2e-58	80.25	DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A5A7UV88	8.4e-58	75.00	DNA-directed RNA polymerase III subunit RPC9 OS=Cucumis melo var. makuwa OX=1194...	[more]

BLAST of Lsi03G001140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548749.1	8.0e-63	82.80	uncharacterized protein LOC111807313 [Cucurbita pepo subsp. pepo] >KAG6574960.1 ...	[more]
XP_023006759.1	1.8e-62	82.80	uncharacterized protein LOC111499396 [Cucurbita maxima] >XP_023006760.1 uncharac...	[more]
XP_022958741.1	2.3e-62	82.17	uncharacterized protein LOC111459926 [Cucurbita moschata]	[more]
XP_038875324.1	5.2e-62	79.63	uncharacterized protein LOC120067805 [Benincasa hispida]	[more]
XP_022138348.1	1.3e-60	80.89	uncharacterized protein LOC111009544 [Momordica charantia]	[more]

BLAST of Lsi03G001140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62950.1	1.2e-32	56.43	RNA polymerase II, Rpb4, core protein	[more]
AT5G62950.3	1.2e-32	56.43	RNA polymerase II, Rpb4, core protein	[more]
AT3G28956.1	1.1e-28	52.82	RNA polymerase II, Rpb4, core protein	[more]
AT5G62950.2	2.3e-28	68.13	RNA polymerase II, Rpb4, core protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006590	RNA polymerase Rpb4/RPC9, core	SMART	SM00657	rpol4neu2	coord: 1..121 e-value: 1.6E-23 score: 94.1
IPR038324	Rpb4/RPC9 superfamily	GENE3D	1.20.1250.40		coord: 1..119 e-value: 2.4E-30 score: 107.1
IPR005574	RNA polymerase subunit RPB4/RPC9	PFAM	PF03874	RNA_pol_Rpb4	coord: 11..117 e-value: 9.2E-22 score: 77.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 131..150
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 118..157
IPR038846	DNA-directed RNA polymerase III subunit RPC9	PANTHER	PTHR15561	CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN	coord: 1..142
IPR010997	HRDC-like superfamily	SUPERFAMILY	47819	HRDC-like	coord: 4..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G001140.1	Lsi03G001140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006384	transcription initiation from RNA polymerase III promoter
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0005666	RNA polymerase III complex
cellular_component	GO:0030880	RNA polymerase complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0000166	nucleotide binding