Lsi02G026420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G026420
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	mitogen-activated protein kinase kinase kinase 2-like
Location	chr02: 32851578 .. 32852879 (-)
RNA-Seq Expression	Lsi02G026420
Synteny	Lsi02G026420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGAAATTCAATGGAAGAAGCTGAAGCAGAAGAAACCCTATTTATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAGCGAGGGCAGCTCATCGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACCATTTTCCCTCCGATAATGGCCGTTAAATCCGCTGAAATTTCCGTCTCCAAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCTTTGATCCAATGCTTCGCCGAAGAGATTACTACCGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTTGCCGCTGGAGGAACCCTAGCTCATCATATCAACAACACAGGTAAATTGATCGAATAAATTACTCTACAATTTAACAAAGAATATTATAATCTCTAGCTTAATTAATCATCCAATTTAATTTCTTACTTAATTTTCTCTCTGCCCTAAACCTAGGGTTATACTAAACTTTTGCCTCTTCTTTCAAATTTTGTTGGTTAATTATACTAATTTATCTAACCCTAACGTTGATAATTTCATACTAAGTATTGATTTCCACTTACTAATATAATAGTGTAAAATTTTGGTGGTTTTCTTAGAAAATTTAATTTATTGGTTGCTGATCTTATTTAAGCAGGTGGAAAGGGTTTACCAGAAAATGAGGTTCGGCGTTACACAAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCAAATATCTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAATCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTGACCGGAAACCTCCCGTGGACCGCCGCGGACAAGGCCGGGATTGTGAAACAGATCACGGAGGATTACGGAATGCCAAAGATTCCGGAAGGGTTGTCGGCGGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGCGTTCAGGTTCACAGCGGAGATGCTGATGACTGTGCCGTTTGTGGCAGGGATTGAAGATCAGGAAGAAGATCAAGATTTTGTGGGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTGA

mRNA sequence

ATGAAGAGAAATTCAATGGAAGAAGCTGAAGCAGAAGAAACCCTATTTATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAGCGAGGGCAGCTCATCGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACCATTTTCCCTCCGATAATGGCCGTTAAATCCGCTGAAATTTCCGTCTCCAAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCTTTGATCCAATGCTTCGCCGAAGAGATTACTACCGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTTGCCGCTGGAGGAACCCTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAGGTTCGGCGTTACACAAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCAAATATCTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAATCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTGACCGGAAACCTCCCGTGGACCGCCGCGGACAAGGCCGGGATTGTGAAACAGATCACGGAGGATTACGGAATGCCAAAGATTCCGGAAGGGTTGTCGGCGGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGCGTTCAGGTTCACAGCGGAGATGCTGATGACTGTGCCGTTTGTGGCAGGGATTGAAGATCAGGAAGAAGATCAAGATTTTGTGGGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTGA

Coding sequence (CDS)

ATGAAGAGAAATTCAATGGAAGAAGCTGAAGCAGAAGAAACCCTATTTATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAGCGAGGGCAGCTCATCGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACCATTTTCCCTCCGATAATGGCCGTTAAATCCGCTGAAATTTCCGTCTCCAAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCTTTGATCCAATGCTTCGCCGAAGAGATTACTACCGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTTGCCGCTGGAGGAACCCTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAGGTTCGGCGTTACACAAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCAAATATCTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAATCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTGACCGGAAACCTCCCGTGGACCGCCGCGGACAAGGCCGGGATTGTGAAACAGATCACGGAGGATTACGGAATGCCAAAGATTCCGGAAGGGTTGTCGGCGGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGCGTTCAGGTTCACAGCGGAGATGCTGATGACTGTGCCGTTTGTGGCAGGGATTGAAGATCAGGAAGAAGATCAAGATTTTGTGGGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTGA

Protein sequence

MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKAGIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV

Homology

[Close]

BLAST of Lsi02G026420 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.9e-52
Identity = 115/292 (39.38%), Postives = 175/292 (59.93%), Query Frame = 0


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           ++W RG+ IG G+F +V  A++  +      FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
            C  +I+C+ E+ T + NG  ++NLLLE A+ G+LA ++   GG+GLPE+ VRR+T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRI----SKTKPSYCL 206
           +GL HIH   + HCD+K ANILL    +        KIAD GLA R+    +  + S  +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 207 GGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKA---GIVKQITEDYG 266
            GT  YMAPE + D+   S +D+WALGC V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 267 MPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQDFV 312
           +PKIPE LS E   FL  CFV++PA R+TAEML+   FV    + +  ++FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFV 281

[Close]

BLAST of Lsi02G026420 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-38
Identity = 102/283 (36.04%), Postives = 157/283 (55.48%), Query Frame = 0


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           ++W RG+++G+GS  +V+ A+   H S       I+AVKS+E+  S+ LQ+E +   +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAA--GHNS-----DEILAVKSSEVHRSEFLQREAKILSSLS 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
               +I     E   + NG ++YNLL+E A  GTL       GG+ + E  V +YT+ I+
Sbjct: 61  S-PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSYCLGGTL 206
           KGL +IH    VHCD+K +N+++    + KG    AKIAD G A+R+     S  + GT 
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVI----SEKGE---AKIADFGCAKRVDPVFESPVM-GTP 180

Query: 207 SYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADK----AGIVKQITEDYGMPK 266
           ++MAPE      Q   SDIWA+GC +++M+TG+ PWT AD       ++ ++      P+
Sbjct: 181 AFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPE 240

Query: 267 IPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQE 306
           +P  L+ EA  FL+ C  R    R+TA  L+  PF+    D E
Sbjct: 241 LPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIE 266

[Close]

BLAST of Lsi02G026420 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-34
Identity = 99/290 (34.14%), Postives = 156/290 (53.79%), Query Frame = 0


Query: 22  SFNNGIQWERGQLIGKGSFGSVFLASRKP-----HISKYTIFPPIMAVKSAEISVSKTLQ 81
           SF    QW++G+LIG+G+FGSV++AS         + +  +FP     KSAE    K L+
Sbjct: 339 SFPMNSQWKKGKLIGRGTFGSVYVASNSETGALCAMKEVELFPD--DPKSAE--CIKQLE 398

Query: 82  KEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPEN 141
           +E +   NL+  N ++Q F  E   D      + + LE    G++  +I +  G  + E+
Sbjct: 399 QEIKLLSNLQHPN-IVQYFGSETVEDR-----FFIYLEYVHPGSINKYIRDHCGT-MTES 458

Query: 142 EVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKT 201
            VR +T+ I+ GL ++H  + VH D+K AN+L+           V K+AD G+A+ ++  
Sbjct: 459 VVRNFTRHILSGLAYLHNKKTVHRDIKGANLLVDASG-------VVKLADFGMAKHLTGQ 518

Query: 202 KPSYCLGGTLSYMAPETLIDSVQESPS--------DIWALGCLVLQMLTGNLPWTAADKA 261
           +    L G+  +MAPE L+ +V +  S        DIW+LGC +++M TG  PW+  + A
Sbjct: 519 RADLSLKGSPYWMAPE-LMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFTGKPPWSEFEGA 578

Query: 262 GIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 299
             + ++  D   P IPE +S E   FL+ CF RNPA R TA ML+   F+
Sbjct: 579 AAMFKVMRD--SPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFL 607

[Close]

BLAST of Lsi02G026420 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 7.3e-32
Identity = 91/296 (30.74%), Postives = 149/296 (50.34%), Query Frame = 0


Query: 24  NNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYD 83
           N+ I+W++GQ++G+G +GSV+L   K           + AVK  EI    +  K K    
Sbjct: 165 NHNIKWQKGQILGRGGYGSVYLGLNKD-------TGELFAVKQLEIVDINSDPKLK---- 224

Query: 84  NLKGCNSLIQCFAEEI----TTDHNGHMIY----------NLLLEVAAGGTLAHHINNTG 143
                 ++I  F++EI    +  H+  + Y          ++ LE   GG+++  +   G
Sbjct: 225 ------NMILSFSKEIEVMRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFG 284

Query: 144 GKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGL 203
                EN ++ YTK I++GL  +H N  +H D+K ANIL+     TKG   + K++D G 
Sbjct: 285 --AFSENVIKVYTKQILQGLSFLHANSIIHRDIKGANILI----DTKG---IVKLSDFGC 344

Query: 204 ARRISKTKPSY-CLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWT-AADK 263
           ++  S     +  + GT  +MAPE +  +     SDIW+LGC++++M T   PW+   + 
Sbjct: 345 SKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITEL 404

Query: 264 AGIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIED 304
           A ++  I     +P IP  +S EA  FL  CF R+P  R  A  L+  PF+  ++D
Sbjct: 405 AAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFIMNLDD 434

[Close]

BLAST of Lsi02G026420 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.9e-30
Identity = 88/282 (31.21%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 27  IQWERGQLIGKGSFGSVF----LASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNY 86
           I+W +G+LIG G+FG V+    L S +    K  +  P  A K       + L++E Q  
Sbjct: 66  IRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLL 125

Query: 87  DNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYT 146
            NL   N +++       +D       N+L+E   GG+++  +   G    PE  +  YT
Sbjct: 126 KNLSHPN-IVRYLGTVRESDS-----LNILMEFVPGGSISSLLEKFG--SFPEPVIIMYT 185

Query: 147 KSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRI---SKTKPS 206
           K ++ GL ++H N  +H D+K ANIL+      KG     ++AD G ++++   +    +
Sbjct: 186 KQLLLGLEYLHNNGIMHRDIKGANILV----DNKG---CIRLADFGASKKVVELATVNGA 245

Query: 207 YCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAA-DKAGIVKQITEDY 266
             + GT  +MAPE ++ +     +DIW++GC V++M TG  PW+    +   V  I    
Sbjct: 246 KSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTK 305

Query: 267 GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAG 301
             P IPE LS EA  FL  C  + P+ R +A  L+  PFV G
Sbjct: 306 AHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTG 332

[Close]

BLAST of Lsi02G026420 vs. ExPASy TrEMBL
Match: A0A0A0KBM3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 9.2e-147
Identity = 266/337 (78.93%), Postives = 290/337 (86.05%), Query Frame = 0


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKR SM E EA +      SS FNNGIQW++G+LIGKGSFGSVFLAS KPH +KY+IFPP
Sbjct: 1   MKRKSMLEKEAAQV--EDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GIVK++TE++ GMPKIPEGLS EA+GFLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of Lsi02G026420 vs. ExPASy TrEMBL
Match: A0A1S3CFZ6 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500025 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.7e-146
Identity = 264/337 (78.34%), Postives = 292/337 (86.65%), Query Frame = 0


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN M E EA +      S+ FNNGIQW+RG+LIGKGSFGSVFLA  KPH +KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQV--EEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNG MIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GI++++TE++ GMPKIPEGLSAEA+GFLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of Lsi02G026420 vs. ExPASy TrEMBL
Match: A0A5D3CH22 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001440 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 6.0e-146
Identity = 265/337 (78.64%), Postives = 290/337 (86.05%), Query Frame = 0


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN M E EA +      SS FNNGIQW+RG+LIGKGSFGSVFLA  KPH  KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQV--EEGSSEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFIKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNG M YNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMTYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GIV+++TE++ GMPKIPEGLSAEA+GFLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGIVQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of Lsi02G026420 vs. ExPASy TrEMBL
Match: A0A5D3CF40 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001430 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.3e-144
Identity = 266/339 (78.47%), Postives = 291/339 (85.84%), Query Frame = 0


Query: 1   MKRNSM-EEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNSM EE E E       SS FNNGIQW+RG+LIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVEVE---DGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           P +MAVKSAEISVS+TLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCIVLEMLT 240

Query: 241 GNLPWTAADKAGIVKQITED-YGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVP 300
           GN  W A DK GIVK++TE+ +GMPKIP+GLS EA+GFLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + + AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

[Close]

BLAST of Lsi02G026420 vs. ExPASy TrEMBL
Match: A0A1S3CED5 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500026 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.6e-144
Identity = 266/339 (78.47%), Postives = 291/339 (85.84%), Query Frame = 0


Query: 1   MKRNSM-EEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNSM EE E E       SS FNNGIQW+RG+LIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVEVE---DGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           P +MAVKSAEISVS+TLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLT 240

Query: 241 GNLPWTAADKAGIVKQITED-YGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVP 300
           GN  W A DK GIVK++TE+ +GMPKIP+GLS EA+GFLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + + AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

[Close]

BLAST of Lsi02G026420 vs. NCBI nr
Match: XP_004136102.2 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus])

HSP 1 Score: 528.5 bits (1360), Expect = 4.3e-146
Identity = 265/337 (78.64%), Postives = 290/337 (86.05%), Query Frame = 0


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKR SM E EA +      SS FNNGIQW++G+LIGKGSFGSVFLAS KPH +KY+IFPP
Sbjct: 1   MKRKSMLEKEAAQV--EDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIH+H++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GIVK++TE++ GMPKIPEGLS EA+GFLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of Lsi02G026420 vs. NCBI nr
Match: XP_008461430.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 528.1 bits (1359), Expect = 5.6e-146
Identity = 264/337 (78.34%), Postives = 292/337 (86.65%), Query Frame = 0


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN M E EA +      S+ FNNGIQW+RG+LIGKGSFGSVFLA  KPH +KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQV--EEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNG MIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GI++++TE++ GMPKIPEGLSAEA+GFLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of Lsi02G026420 vs. NCBI nr
Match: KAA0058688.1 (mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa] >TYK10494.1 mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 526.9 bits (1356), Expect = 1.2e-145
Identity = 265/337 (78.64%), Postives = 290/337 (86.05%), Query Frame = 0


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN M E EA +      SS FNNGIQW+RG+LIGKGSFGSVFLA  KPH  KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQV--EEGSSEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFIKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNG M YNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMTYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GIV+++TE++ GMPKIPEGLSAEA+GFLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGIVQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of Lsi02G026420 vs. NCBI nr
Match: KAE8646420.1 (hypothetical protein Csa_015819 [Cucumis sativus])

HSP 1 Score: 523.1 bits (1346), Expect = 1.8e-144
Identity = 257/318 (80.82%), Postives = 281/318 (88.36%), Query Frame = 0


Query: 20  SSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEK 79
           SS FNNGIQW++G+LIGKGSFGSVFLAS KPH +KY+IFPP+MAVKSAEISVS+TLQKEK
Sbjct: 13  SSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVMAVKSAEISVSETLQKEK 72

Query: 80  QNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVR 139
           QNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGTLAHHI NTGGKGL EN VR
Sbjct: 73  QNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLAHHIKNTGGKGLEENVVR 132

Query: 140 RYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRRFVAKIADLGLARRISKTKP 199
            YTKSIIKGLIH+H++QYVHCDLKPANILL+P+ NTTK R+F+AKIADLGLARR SKTK 
Sbjct: 133 NYTKSIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIADLGLARRTSKTKA 192

Query: 200 SYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKAGIVKQITEDY 259
           SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN  W A DK GIVK++TE++
Sbjct: 193 SYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRAWAATDKVGIVKEMTENF 252

Query: 260 -GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQDFVGAPSFV 319
            GMPKIPEGLS EA+GFLKNC VR P FRFTAEMLM VPFVA  ED E+D   V AP+FV
Sbjct: 253 IGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFVAAGEDHEQDSTTVKAPTFV 312

Query: 320 KKRPKQFKRQRIIPIKAV 336
            K P+QFKRQR IPIKAV
Sbjct: 313 TKWPRQFKRQRTIPIKAV 330

[Close]

BLAST of Lsi02G026420 vs. NCBI nr
Match: KAA0058687.1 (mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa] >TYK10493.1 mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 520.0 bits (1338), Expect = 1.5e-143
Identity = 266/339 (78.47%), Postives = 291/339 (85.84%), Query Frame = 0


Query: 1   MKRNSM-EEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNSM EE E E       SS FNNGIQW+RG+LIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVEVE---DGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           P +MAVKSAEISVS+TLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCIVLEMLT 240

Query: 241 GNLPWTAADKAGIVKQITED-YGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVP 300
           GN  W A DK GIVK++TE+ +GMPKIP+GLS EA+GFLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + + AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

[Close]

BLAST of Lsi02G026420 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 210.3 bits (534), Expect = 2.4e-54
Identity = 122/295 (41.36%), Postives = 179/295 (60.68%), Query Frame = 0


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNL- 86
           ++W RG+ IG G+F +V LA+R  + S    FPP+MAVKSA+   + +L  EK   DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGE--FPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 87  KGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSI 146
             CN +++CF E+ T + NG  ++NL LE A+ G+L  ++    G+G+PE+ VRR+T S+
Sbjct: 61  DDCNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 147 IKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISK-TKPSY--CL 206
           ++GL HIH N + HCDLK  NILL             KIAD GLA+RI   T  +Y   +
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDG-------AVKIADFGLAKRIGDLTALNYGVQI 180

Query: 207 GGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKA---GIVKQITEDYG 266
            GT  YMAPE++ D+   S  D+WALGC+V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDE 240

Query: 267 MPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQE---EDQDFV 312
           +P IPE LS +   FL  CFV++P  R+TAEML+  PFV    D +   +++DFV
Sbjct: 241 VPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFV 285

[Close]

BLAST of Lsi02G026420 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 209.5 bits (532), Expect = 4.1e-54
Identity = 121/288 (42.01%), Postives = 174/288 (60.42%), Query Frame = 0


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           ++W R + IG GSF +V LA+     SK   FP +MAVKS+ +  S  L+ E+   D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
            C+ +++CF E  T + NG  IYNL LE A+GG+LA  I ++ G+ LPE EVRR+T+SI+
Sbjct: 61  DCSEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSS-GEALPEFEVRRFTRSIV 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSYCLGGTL 206
           KGL HIH N + HCD+K  N+L+             KI+D GLA+R S  +    + GT 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDGD-------VKISDFGLAKRRS-GEVCVEIRGTP 180

Query: 207 SYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKA-----GIVKQITEDYGMP 266
            YMAPE++     ESP+DIWALGC V++M +G   W   D        ++ +I     +P
Sbjct: 181 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 240

Query: 267 KIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQD 310
           +IP  LS E   F+  CFV+N A R+TAEML+  PF+A ++D+  ++D
Sbjct: 241 RIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLA-VDDESGEED 275

[Close]

BLAST of Lsi02G026420 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 206.5 bits (524), Expect = 3.4e-53
Identity = 115/292 (39.38%), Postives = 175/292 (59.93%), Query Frame = 0


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           ++W RG+ IG G+F +V  A++  +      FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
            C  +I+C+ E+ T + NG  ++NLLLE A+ G+LA ++   GG+GLPE+ VRR+T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRI----SKTKPSYCL 206
           +GL HIH   + HCD+K ANILL    +        KIAD GLA R+    +  + S  +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 207 GGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKA---GIVKQITEDYG 266
            GT  YMAPE + D+   S +D+WALGC V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 267 MPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQDFV 312
           +PKIPE LS E   FL  CFV++PA R+TAEML+   FV    + +  ++FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFV 281

[Close]

BLAST of Lsi02G026420 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 178.3 bits (451), Expect = 1.0e-44
Identity = 105/283 (37.10%), Postives = 158/283 (55.83%), Query Frame = 0


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           I W RG +IG+GS  +V +A         +    + AVKSA++S S  LQKE+     L 
Sbjct: 3   INWTRGPIIGRGSTATVSIA--------ISSSGELFAVKSADLSSSSLLQKEQSILSTLS 62

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
             + +++     +T + NG ++YN+L+E  +GG L   I N+GGK LPE E+R YT+ I+
Sbjct: 63  SPH-MVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSYCLGGTL 206
            GL+++H+   VHCDLK  N+L+           V KIAD+G A+ + K++      GT 
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLVEENG-------VLKIADMGCAKSVDKSE----FSGTP 182

Query: 207 SYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAA-DKAGIVKQITEDYGMPKIPE 266
           ++MAPE      Q  P+D+WALGC +++M+TG+ PW    D    + +I      P IP 
Sbjct: 183 AFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPA 242

Query: 267 GLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQ 309
            +S +A  FLKNC   +   R+T E L+  PF   ++D EE Q
Sbjct: 243 WISDKAKDFLKNCLKEDQKQRWTVEELLKHPF---LDDDEESQ 260

[Close]

BLAST of Lsi02G026420 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.4e-41
Identity = 95/242 (39.26%), Postives = 137/242 (56.61%), Query Frame = 0


Query: 63  AVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITT--DHNGHMIYNLLLEVAAGGT 122
           AVKS++     +L KE      LKGC  +IQCF  ++    D  G+ +Y LLLE A+ G+
Sbjct: 35  AVKSSD--DENSLLKEFHILSELKGCPRIIQCFGNDLEEGFDDKGNRVYKLLLEYASEGS 94

Query: 123 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRF 182
           L+  +NN   + LP+  +R +T+ I++GL+ IH + YVHCDLKP N+L+ P     G  +
Sbjct: 95  LSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIHSHGYVHCDLKPENVLVFP----CGDSY 154

Query: 183 VAKIADLGLARRISKT----KPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQML 242
             KI+D GL+ ++ +     K  Y   GTL+YM PE+L D V     D+W+LGCLVL+M 
Sbjct: 155 EVKISDFGLSLQVGEVPDHWKIEYPFVGTLNYMPPESLHDGVANKTLDLWSLGCLVLEMY 214

Query: 243 TGNLPWTAADKAGIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVP 299
               PW        V  I  +   P+IPE L  +A  F++ CF RNP  R TA  L++  
Sbjct: 215 VCKKPWIGFIPEDFV-YILSNGNPPEIPESLPCDARAFIQKCFSRNPKERGTASELLSHR 269

The following BLAST results are available for this feature:

BLAST of Lsi02G026420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	4.9e-52	39.38	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	2.3e-38	36.04	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9C5H5	2.0e-34	34.14	Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702...	[more]
Q54R82	7.3e-32	30.74	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
O22042	8.9e-30	31.21	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Lsi02G026420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBM3	9.2e-147	78.93	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430...	[more]
A0A1S3CFZ6	2.7e-146	78.34	mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN...	[more]
A0A5D3CH22	6.0e-146	78.64	Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw...	[more]
A0A5D3CF40	7.3e-144	78.47	Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw...	[more]
A0A1S3CED5	9.6e-144	78.47	mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN...	[more]

BLAST of Lsi02G026420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136102.2	4.3e-146	78.64	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus]	[more]
XP_008461430.1	5.6e-146	78.34	PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo]	[more]
KAA0058688.1	1.2e-145	78.64	mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa]...	[more]
KAE8646420.1	1.8e-144	80.82	hypothetical protein Csa_015819 [Cucumis sativus]	[more]
KAA0058687.1	1.5e-143	78.47	mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa]...	[more]

BLAST of Lsi02G026420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67080.1	2.4e-54	41.36	mitogen-activated protein kinase kinase kinase 19	[more]
AT4G36950.1	4.1e-54	42.01	mitogen-activated protein kinase kinase kinase 21	[more]
AT3G50310.1	3.4e-53	39.38	mitogen-activated protein kinase kinase kinase 20	[more]
AT4G26890.1	1.0e-44	37.10	mitogen-activated protein kinase kinase kinase 16	[more]
AT5G27510.1	1.4e-41	39.26	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 29..298 e-value: 4.2E-54 score: 195.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 30..297 e-value: 2.7E-45 score: 154.8
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 29..298 score: 36.866432
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 25..330 e-value: 7.8E-55 score: 188.3
None	No IPR available	PIRSR	PIRSR037014-2	PIRSR037014-2	coord: 106..288 e-value: 1.9E-10 score: 37.5
None	No IPR available	PIRSR	PIRSR037014-1	PIRSR037014-1	coord: 106..288 e-value: 1.9E-10 score: 37.5
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 26..310
None	No IPR available	PANTHER	PTHR48011:SF56	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKE	coord: 26..310
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 157..169
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 35..65
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 28..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G026420.1	Lsi02G026420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity