Lsi02G026260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G026260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	chr02: 32687522 .. 32689219 (+)
RNA-Seq Expression	Lsi02G026260
Synteny	Lsi02G026260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGATTTCATTTGACTTGGAAATGGATTTTGTTAACGCTGCTGCTAACCTCACCGACGGTGAGCTTTGTAGGTGGAGATGATGAGAAAGACAAAGAGGAATGCACGCAGCAATTGGCTGGGATGGCCACATGTCTGCCGTATGTGAGCGGTGATGCAAAGGCTCCGACGCCTGACTGTTGCACTGGACTGAAGGAAGTGCTTAAGAACAACAAGAAATGCTTATGCGTTATAGTAAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATAAATGTCACACTGGCCTTGGGCCTGCCTGATACTTGCCATGCCACTGCCAATGTCTCCAATTGTCCAGGTAAATTCAAGATAAAGTTTATCCGTGATAGATTTCTATCGCTGATAGACCACCTAGACCATAAAAATCTATCATTGATAAACTTTGTTATATTTATAATTTTTTTTTTAAAATGTTGCTATATATGCTAATACTTTGAATCTGATTGTTATATATGCAACTGTCCCTAATCAAATTTTACATGATTTTATTTTATTTGTTTTGGTTAGATATATGTTTTAGTCCTTAAAGTTTAAGTTTTTCGATTTATTTTTTTAGTCCCTATCATTTACAATGTTTAGATTTAGTTTTGTATGTACATGTCTAACTAGGCGCTTGAAGTGGAAAGGAGTGGAGTTGTTTAGTATGAAAAATTGTTTGATCGTAGTTAATAAGTCATGAAATTGATATTTTTTTAGTTATGGGACCCATAAACTCTTTGGGGGCATGTCTTGAGAATTCCGCCTCTCATCGATGAAAGTTTAAATTGGCTGATAACAAGTTGACAAAATATAATGTTCAGTAAATTGACACAATAAAGATTAAAAAAAAAAAAAATCATCAACTAGATGAAAGTTTGAAAATTTCTACCGAATTCTTAAAAGTTTGGAGGAATTAAGAAACTCTTATCTCTCATATTCTTTAGACGAAGAATTAGAACTTGAGATGTTGGTTAATGATAACTATACATTGCATAATCTTAAGCTTAACATTAGTTAATCTAACAATCACTCATATGAAAGATATAATTGGAAAATATGAAAAGTGTGGGTTTAAATTGTAACACATTCAAGTTTAAGGATTAAATAAAAGTATATAGACTAAATTGTTACAAATCAAATATAGAGATTAAATTGTTACTTTTACGAAAGTTTGAGACTAAAAATGATTCTTAACCAACGTATATAAATGGGGTTGATGCAGCTCTTTTAAACTTGCCACCCAATTCAGCAGAGGCTCAAGTTTTCTATCAATTGGGCAAGGGAAATAGCTCCTCTGCTGTAGCAGCTGCAGCGCCCATAAGCCCTTCTCCTACCATTAATTCCACAGGTAATTTGGCTCTTTCAATTAATTTTATTTATTATTATAATAATAAAGTTTGGAAAAGAAATTTATGGTCATTAATGGTTTTTTTATTTTTATTTTTTATTCTTCTTCTTCACCAAAATTGCAGTTGGAGGGGGAAGTAGAATTGGGCAAGAGAAGGGTGAAGCTTACAACATAGGAAAGAAATGGCTGCCATTAGAGACTTTGGTTGTTGGAGCTCTATTGCTTTTGTTTTGGTTGGACTCATACCATGTTTAGAACAGACTGTTTCAGCATATTCATGCTTTTATATCTATCACATATAATATAATATTCGACGTTTTTGGTCT

mRNA sequence

ATGGAGGGATTTCATTTGACTTGGAAATGGATTTTGTTAACGCTGCTGCTAACCTCACCGACGGTGAGCTTTGTAGGTGGAGATGATGAGAAAGACAAAGAGGAATGCACGCAGCAATTGGCTGGGATGGCCACATGTCTGCCGTATGTGAGCGGTGATGCAAAGGCTCCGACGCCTGACTGTTGCACTGGACTGAAGGAAGTGCTTAAGAACAACAAGAAATGCTTATGCGTTATAGTAAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATAAATGGAAATAGCTCCTCTGCTGTAGCAGCTGCAGCGCCCATAAGCCCTTCTCCTACCATTAATTCCACAGTTGGAGGGGGAAGTAGAATTGGGCAAGAGAAGGGTGAAGCTTACAACATAGGAAAGAAATGGCTGCCATTAGAGACTTTGGTTGTTGGAGCTCTATTGCTTTTGTTTTGGTTGGACTCATACCATGTTTAGAACAGACTGTTTCAGCATATTCATGCTTTTATATCTATCACATATAATATAATATTCGACGTTTTTGGTCT

Coding sequence (CDS)

ATGGAGGGATTTCATTTGACTTGGAAATGGATTTTGTTAACGCTGCTGCTAACCTCACCGACGGTGAGCTTTGTAGGTGGAGATGATGAGAAAGACAAAGAGGAATGCACGCAGCAATTGGCTGGGATGGCCACATGTCTGCCGTATGTGAGCGGTGATGCAAAGGCTCCGACGCCTGACTGTTGCACTGGACTGAAGGAAGTGCTTAAGAACAACAAGAAATGCTTATGCGTTATAGTAAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATAAATGGAAATAGCTCCTCTGCTGTAGCAGCTGCAGCGCCCATAAGCCCTTCTCCTACCATTAATTCCACAGTTGGAGGGGGAAGTAGAATTGGGCAAGAGAAGGGTGAAGCTTACAACATAGGAAAGAAATGGCTGCCATTAGAGACTTTGGTTGTTGGAGCTCTATTGCTTTTGTTTTGGTTGGACTCATACCATGTTTAG

Protein sequence

MEGFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLKNNKKCLCVIVKDRNDPDLGLQINGNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLETLVVGALLLLFWLDSYHV

Homology

[Close]

BLAST of Lsi02G026260 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.9e-28
Identity = 60/101 (59.41%), Postives = 74/101 (73.27%), Query Frame = 0


Query: 16  LLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLKNNKKC 75
           L+    V     D  KDKEECT+QL GMATCLPYV G AK+PTPDCC+GLK+VL +NKKC
Sbjct: 15  LIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNKKC 74

Query: 76  LCVIVKDRNDPDLGLQINGNSSSAVA----AAAPISPSPTI 113
           LCVI++DRNDPDLGLQIN + + A+     AAA ++  P +
Sbjct: 75  LCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPAL 115

[Close]

BLAST of Lsi02G026260 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-26
Identity = 55/104 (52.88%), Postives = 75/104 (72.12%), Query Frame = 0


Query: 13  LTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLKNN 72
           + L++ +  V   G D  KDKEECT QL GMATCLPYV G AK+PTPDCC+GLK+V+ ++
Sbjct: 13  IALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSD 72

Query: 73  KKCLCVIVKDRNDPDLGLQINGNSSSAVA----AAAPISPSPTI 113
            KCLC+I+++RNDPDLGLQ+N + + A+     A A I+  P +
Sbjct: 73  MKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPAL 116

[Close]

BLAST of Lsi02G026260 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 2.5e-22
Identity = 44/106 (41.51%), Postives = 75/106 (70.75%), Query Frame = 0


Query: 11  ILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLK 70
           + +T+++TS  + F   D  +D+EECT QL  ++TC+PYV GDAKAPT DCC G  +V++
Sbjct: 8   LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 67

Query: 71  NNKKCLCVIVKDRNDPDLGLQINGNSSSAVAAAAPISPSPTINSTV 117
            ++KC+C++V+D++DP LG++IN   ++ + +A  I+ +P I   +
Sbjct: 68  KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHIT-APNITDCI 112

[Close]

BLAST of Lsi02G026260 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.7e-11
Identity = 35/99 (35.35%), Postives = 59/99 (59.60%), Query Frame = 0


Query: 12  LLTLLLTSPTVSFVGGDDE--KDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVL 71
           +L L +T   V F+G   E  +D + C   ++ + +CLP+V+  AKAP   CC+ LK  +
Sbjct: 13  MLALCIT-VAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKI 72

Query: 72  K--NNKKCLCVIVKDRNDPDLGLQINGNSSSAVAAAAPI 107
                +KCLC +VKDR+DP LG +++ N + ++ +A  +
Sbjct: 73  DKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHV 110

[Close]

BLAST of Lsi02G026260 vs. ExPASy Swiss-Prot
Match: Q9LJ86 (Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=3702 GN=LTPG5 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 8.5e-07
Identity = 24/87 (27.59%), Postives = 43/87 (49.43%), Query Frame = 0


Query: 33  KEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLKNNKKCLCVIVKDRNDPDLGLQI 92
           +  CT  L  M+ CL Y++G++ +P   CC  L  V++++  CLC ++ +     LG+ +
Sbjct: 25  QSSCTNALISMSPCLNYITGNSTSPNQQCCNQLSRVVQSSPDCLCQVL-NGGGSQLGINV 84

Query: 93  NGNSSSAVAAAAPISPSPTINSTVGGG 120
           N   +  +  A  +   P      GGG
Sbjct: 85  NQTQALGLPRACNVQTPPVSRCNTGGG 110

[Close]

BLAST of Lsi02G026260 vs. ExPASy TrEMBL
Match: A0A6J1KZ76 (protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.2e-58
Identity = 123/195 (63.08%), Postives = 134/195 (68.72%), Query Frame = 0


Query: 3   GFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCC 62
           GFH   KW+LLTLLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSGDAKAPTPDCC
Sbjct: 2   GFHFGCKWLLLTLLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61

Query: 63  TGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN----------------------------- 122
           +GLK VLK+NKKCLCVIVKDRNDPDLGLQIN                             
Sbjct: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSTCPALLNLPPS 121

Query: 123 ----------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLETLV 159
                     G + S+ AAAAPISPSP INST GGGSR G +KGEAY +G KWLPL+TL 
Sbjct: 122 SPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTVGNKWLPLKTLF 181

[Close]

BLAST of Lsi02G026260 vs. ExPASy TrEMBL
Match: A0A6J1H6P3 (protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 9.3e-57
Identity = 120/192 (62.50%), Postives = 132/192 (68.75%), Query Frame = 0


Query: 3   GFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCC 62
           GFH   KW+LL LLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSGDAKAPTPDCC
Sbjct: 2   GFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61

Query: 63  TGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN----------------------------- 122
           +GLK VLK+NKKCLCVIVKDRNDPDLGLQIN                             
Sbjct: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPS 121

Query: 123 ----------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLETLV 156
                     G + S+ AAAAPISPSP I+ST GGGSR G +KGEAY IG KWLPL++LV
Sbjct: 122 SPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSLV 181

[Close]

BLAST of Lsi02G026260 vs. ExPASy TrEMBL
Match: A0A6J1D8S3 (protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.3e-55
Identity = 122/197 (61.93%), Postives = 132/197 (67.01%), Query Frame = 0


Query: 4   FHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCT 63
           FH  +K +LLTLLLTSP V  V  D EKDKEECTQQLAGMATCLPYVSGDAK+PTPDCC 
Sbjct: 3   FHFAFKLLLLTLLLTSPAVRLVTADAEKDKEECTQQLAGMATCLPYVSGDAKSPTPDCCN 62

Query: 64  GLKEVLKNNKKCLCVIVKDRNDPDLGLQIN------------------------------ 123
           GLKEVLK+NKKCLCVIVKDRNDPDLGLQIN                              
Sbjct: 63  GLKEVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDSCHASANVSNCPALLNLPPNS 122

Query: 124 ------------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLET 159
                        +SS+A +AAAPISPS T NSTVGGGSRIG +KGEA   G KWLPLE 
Sbjct: 123 PEAQVFYQLGKSNSSSAAASAAAPISPSSTTNSTVGGGSRIGPDKGEACYRGNKWLPLEN 182

[Close]

BLAST of Lsi02G026260 vs. ExPASy TrEMBL
Match: A0A1S3CDS0 (protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.0e-47
Identity = 116/194 (59.79%), Postives = 123/194 (63.40%), Query Frame = 0


Query: 1   MEGFHLTWKWILLTLLLTSP-TVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTP 60
           MEGF L +KW LL LLL SP +V FVGGDD+KDKEECT QLAGMATCLPYVSGDAK PTP
Sbjct: 1   MEGFRLCFKWFLLMLLLRSPSSVRFVGGDDKKDKEECTPQLAGMATCLPYVSGDAKTPTP 60

Query: 61  DCCTGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN-------------------------- 120
           DCC+GLKEVLKNNKKCLCVIVKDRNDPDLGLQIN                          
Sbjct: 61  DCCSGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNL 120

Query: 121 ---------------GNSSSAVAAAAPISPS--PTINSTVGGGSRIGQEKGEAYNIGKKW 151
                          G SSSA+A A  ISPS   TI+STVGGGS     K   Y IGKKW
Sbjct: 121 PSNSSEAQVFYQLGKGKSSSALAPAPIISPSSPATISSTVGGGS----TKSGGYKIGKKW 180

[Close]

BLAST of Lsi02G026260 vs. ExPASy TrEMBL
Match: A0A0A0K6P6 (Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.8e-45
Identity = 110/196 (56.12%), Postives = 124/196 (63.27%), Query Frame = 0


Query: 1   MEG---FHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAP 60
           MEG   F   W W+++ LL +   V FVGGDD+KD+EECT QLAGMATCLPYVSGDAKAP
Sbjct: 1   MEGCRRFSFKWLWLIMLLLRSPSWVRFVGGDDKKDREECTPQLAGMATCLPYVSGDAKAP 60

Query: 61  TPDCCTGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN------------------------ 120
           TPDCC+GLKEVL+N+KKCLCVIV+DRNDPDLGLQIN                        
Sbjct: 61  TPDCCSGLKEVLQNDKKCLCVIVRDRNDPDLGLQINVTLALSLPDICHATANVSNCPALL 120

Query: 121 -----------------GNSSSAVAAAAPISP--SPTINSTVGGGSRIGQEKGEAYNIGK 151
                            G SSSA+A A  +SP  SPTINSTVGGGS+ G E GE Y  GK
Sbjct: 121 NMPSNSSDAQVFYQLGKGKSSSALAPAPMLSPSSSPTINSTVGGGSKSGAE-GEGYKRGK 180

[Close]

BLAST of Lsi02G026260 vs. NCBI nr
Match: XP_038899115.1 (non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida])

HSP 1 Score: 287.3 bits (734), Expect = 7.8e-74
Identity = 145/199 (72.86%), Postives = 150/199 (75.38%), Query Frame = 0


Query: 1   MEGFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPD 60
           MEGFH TWKW+LLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSG AKAPTPD
Sbjct: 1   MEGFHFTWKWLLLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGGAKAPTPD 60

Query: 61  CCTGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN--------------------------- 120
           CC+GLKEVLKNNKKCLCVI+KDRNDPDLGLQIN                           
Sbjct: 61  CCSGLKEVLKNNKKCLCVIIKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNLP 120

Query: 121 --------------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPL 159
                         GNSSS+  A APISPSPTINSTVGGGSRIG +KG AYNIGKKWLPL
Sbjct: 121 PNSAEAQVFYQLGKGNSSSSAVAPAPISPSPTINSTVGGGSRIGPDKGGAYNIGKKWLPL 180

[Close]

BLAST of Lsi02G026260 vs. NCBI nr
Match: KAG6593339.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235.0 bits (598), Expect = 4.6e-58
Identity = 125/195 (64.10%), Postives = 135/195 (69.23%), Query Frame = 0


Query: 3   GFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCC 62
           GFH   KW+LL LLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSGDAKAPTPDCC
Sbjct: 2   GFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61

Query: 63  TGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN----------------------------- 122
           +GLK VLK+NKKCLCVIVKDRNDPDLGLQIN                             
Sbjct: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPS 121

Query: 123 ----------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLETLV 159
                     G + S+ AAAAPISPSP INST GGGSR G +KGEAY IG KWLPL+TLV
Sbjct: 122 SPEAQVFYQFGKAQSSSAAAAPISPSPAINST-GGGSRNGTDKGEAYTIGNKWLPLKTLV 181

[Close]

BLAST of Lsi02G026260 vs. NCBI nr
Match: XP_023004343.1 (protein YLS3-like [Cucurbita maxima])

HSP 1 Score: 235.0 bits (598), Expect = 4.6e-58
Identity = 123/195 (63.08%), Postives = 134/195 (68.72%), Query Frame = 0


Query: 3   GFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCC 62
           GFH   KW+LLTLLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSGDAKAPTPDCC
Sbjct: 2   GFHFGCKWLLLTLLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61

Query: 63  TGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN----------------------------- 122
           +GLK VLK+NKKCLCVIVKDRNDPDLGLQIN                             
Sbjct: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSTCPALLNLPPS 121

Query: 123 ----------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLETLV 159
                     G + S+ AAAAPISPSP INST GGGSR G +KGEAY +G KWLPL+TL 
Sbjct: 122 SPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTVGNKWLPLKTLF 181

[Close]

BLAST of Lsi02G026260 vs. NCBI nr
Match: XP_023513729.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 230.7 bits (587), Expect = 8.6e-57
Identity = 121/192 (63.02%), Postives = 132/192 (68.75%), Query Frame = 0


Query: 3   GFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCC 62
           GFH   KW+LL LLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSGDAKAPTPDCC
Sbjct: 2   GFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61

Query: 63  TGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN----------------------------- 122
           +GLK VLK+NKKCLCVIVKDRNDPDLGLQIN                             
Sbjct: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPN 121

Query: 123 ----------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLETLV 156
                     G + S+ AAAAPISPSP INST GGGSR G +KGEAY IG KWLPL++LV
Sbjct: 122 SPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTIGNKWLPLKSLV 181

[Close]

BLAST of Lsi02G026260 vs. NCBI nr
Match: XP_022959693.1 (protein YLS3-like [Cucurbita moschata])

HSP 1 Score: 229.6 bits (584), Expect = 1.9e-56
Identity = 120/192 (62.50%), Postives = 132/192 (68.75%), Query Frame = 0


Query: 3   GFHLTWKWILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCC 62
           GFH   KW+LL LLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSGDAKAPTPDCC
Sbjct: 2   GFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61

Query: 63  TGLKEVLKNNKKCLCVIVKDRNDPDLGLQIN----------------------------- 122
           +GLK VLK+NKKCLCVIVKDRNDPDLGLQIN                             
Sbjct: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPS 121

Query: 123 ----------GNSSSAVAAAAPISPSPTINSTVGGGSRIGQEKGEAYNIGKKWLPLETLV 156
                     G + S+ AAAAPISPSP I+ST GGGSR G +KGEAY IG KWLPL++LV
Sbjct: 122 SPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSLV 181

[Close]

BLAST of Lsi02G026260 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 125.9 bits (315), Expect = 2.8e-29
Identity = 60/101 (59.41%), Postives = 74/101 (73.27%), Query Frame = 0


Query: 16  LLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLKNNKKC 75
           L+    V     D  KDKEECT+QL GMATCLPYV G AK+PTPDCC+GLK+VL +NKKC
Sbjct: 15  LIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNKKC 74

Query: 76  LCVIVKDRNDPDLGLQINGNSSSAVA----AAAPISPSPTI 113
           LCVI++DRNDPDLGLQIN + + A+     AAA ++  P +
Sbjct: 75  LCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPAL 115

[Close]

BLAST of Lsi02G026260 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 9.0e-28
Identity = 55/104 (52.88%), Postives = 75/104 (72.12%), Query Frame = 0


Query: 13  LTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLKNN 72
           + L++ +  V   G D  KDKEECT QL GMATCLPYV G AK+PTPDCC+GLK+V+ ++
Sbjct: 13  IALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSD 72

Query: 73  KKCLCVIVKDRNDPDLGLQINGNSSSAVA----AAAPISPSPTI 113
            KCLC+I+++RNDPDLGLQ+N + + A+     A A I+  P +
Sbjct: 73  MKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPAL 116

[Close]

BLAST of Lsi02G026260 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 1.8e-23
Identity = 44/106 (41.51%), Postives = 75/106 (70.75%), Query Frame = 0


Query: 11  ILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLK 70
           + +T+++TS  + F   D  +D+EECT QL  ++TC+PYV GDAKAPT DCC G  +V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 71  NNKKCLCVIVKDRNDPDLGLQINGNSSSAVAAAAPISPSPTINSTV 117
            ++KC+C++V+D++DP LG++IN   ++ + +A  I+ +P I   +
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHIT-APNITDCI 155

[Close]

BLAST of Lsi02G026260 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 1.8e-23
Identity = 44/106 (41.51%), Postives = 75/106 (70.75%), Query Frame = 0


Query: 11  ILLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVLK 70
           + +T+++TS  + F   D  +D+EECT QL  ++TC+PYV GDAKAPT DCC G  +V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 71  NNKKCLCVIVKDRNDPDLGLQINGNSSSAVAAAAPISPSPTINSTV 117
            ++KC+C++V+D++DP LG++IN   ++ + +A  I+ +P I   +
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHIT-APNITDCI 155

[Close]

BLAST of Lsi02G026260 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 6.9e-12
Identity = 35/99 (35.35%), Postives = 59/99 (59.60%), Query Frame = 0


Query: 12  LLTLLLTSPTVSFVGGDDE--KDKEECTQQLAGMATCLPYVSGDAKAPTPDCCTGLKEVL 71
           +L L +T   V F+G   E  +D + C   ++ + +CLP+V+  AKAP   CC+ LK  +
Sbjct: 13  MLALCIT-VAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKI 72

Query: 72  K--NNKKCLCVIVKDRNDPDLGLQINGNSSSAVAAAAPI 107
                +KCLC +VKDR+DP LG +++ N + ++ +A  +
Sbjct: 73  DKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHV 110

The following BLAST results are available for this feature:

BLAST of Lsi02G026260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64865	3.9e-28	59.41	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
O64864	1.3e-26	52.88	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
F4I082	2.5e-22	41.51	Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37...	[more]
Q9M2G1	9.7e-11	35.35	Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3...	[more]
Q9LJ86	8.5e-07	27.59	Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Lsi02G026260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KZ76	2.2e-58	63.08	protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1	[more]
A0A6J1H6P3	9.3e-57	62.50	protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1	[more]
A0A6J1D8S3	1.3e-55	61.93	protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1	[more]
A0A1S3CDS0	1.0e-47	59.79	protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1	[more]
A0A0A0K6P6	4.8e-45	56.12	Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 P...	[more]

BLAST of Lsi02G026260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899115.1	7.8e-74	72.86	non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida]	[more]
KAG6593339.1	4.6e-58	64.10	Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Prot...	[more]
XP_023004343.1	4.6e-58	63.08	protein YLS3-like [Cucurbita maxima]	[more]
XP_023513729.1	8.6e-57	63.02	protein YLS3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022959693.1	1.9e-56	62.50	protein YLS3-like [Cucurbita moschata]	[more]

BLAST of Lsi02G026260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44300.1	2.8e-29	59.41	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	9.0e-28	52.88	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.1	1.8e-23	41.51	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.2	1.8e-23	41.51	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G58550.1	6.9e-12	35.35	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 29..103 e-value: 4.7E-11 score: 42.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 33..110 e-value: 2.7E-11 score: 45.2
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 35..89
None	No IPR available	PANTHER	PTHR33044:SF94	PROTEIN YLS3	coord: 14..96
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 43..90 e-value: 1.00369E-13 score: 60.5183
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 14..96

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G026260.1	Lsi02G026260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008643	carbohydrate transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0015293	symporter activity