Lsi02G024620 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G024620
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionLipase_3 domain-containing protein
Locationchr02: 31121448 .. 31124928 (+)
RNA-Seq ExpressionLsi02G024620
SyntenyLsi02G024620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGTAGGTGACCTGTTTGGATACGGAAATAGCATGAACCTCGTCTTGGCAAGTCTATAAGAACACAATTCCTGATTTCTCTCTCTGCCTCTCTCTTGCTCTAAATTTCTGTTTACGAATTTCTCGTCGGGGTTCAACAAGACAACCCAAATTCAAAGATCGTGGTGATCGTCGGAGTCTTTTTTATAGGCTAATTCTCGCGTGAATCTCGTTTTTAAATATCGGAATCTGATTCTGATTCGTGATCGATTAATCAATTTTTGTTGTTGTTAGAAACAGAGTATTCTATTGGATTTTGAGAAAACGAAAATAATGGATAGCCTCTGTTTGAATCCTGGAATACATGGGATTGCTTCGTCTTTATCAGTGAATGCGGCTCTTGATGTTCGTGTAAATCCGTCTCAGGTCAGTACGACCGGTAGGTCGTCGTCGTCGGCAGCGGTTGAGAAATCGTCGAAAACAATATCTCCGTCTCCGTCTCCGTCTCCGTCGCCATCGTCTTCGTCTTCATCGTTTCTGAAGTTTTCTTTGAAGTATCCATTGCAATCGCTGTGGAGTCGGAGTGGTGAAAGTGGGAATTCGAGGCGTAGTGGTTTGGCGCTTGACGACGCCGTTCTGATGGACAGTGAAGACGATCGGAGAGTCGTTTGTGAGGAGGAAAGTGAAAATGTAGCAACCGGATCGGACTGGAGAAATGCAAACTGGGTAATGAAGATTTTGCGGGTGAGATCTCTGTGGAGAGAGGAGGAGAAGCAGGGAATTGCAGAAGATGAGTTTAGAGAGGAGAGGGAGGAGGACCGTGTGGTGGAAGATCGAGAAATTTCTTGTGATGACGAGGAATTTTGTGATACTTGCGGAATCGTTGAGGAAGAAGATGAAAAGGAGGTTGAATTTGATAAACATTCGTTTTCGAGATTGCTTCGACGGGTTTCCTTGGCTGAAGCGAGGTTATATGCTCAAATGTCGTATTTAGGGTGCCTCGCATACTCCATTTCCGAAATTAAGGTACAGAATTTAATTCAATTACGATAATTTTGTTATTTGTTTTATAAATTAGAGAACCCCAGGTTAAGATTTGTATGCTAGTCAAGAAATCTGGTTGGCTGTCAACAATAAATTGCTTCATTCTCCAATGAAAATTTACAATTATAAGAATTAAGAAACAATCAGGAATGCATCTGCTCTGTTGCTCTGTTGGTTTATCAAATCAGTTTTTCTTTTGTTCAATCTTTTGTTCGTTTTAGTTTCTTTTAATTACATGTTCTTGAGCTTCAGCTAGCTTGATAAACTAAAGTTTTGCTTCTTTTGTTGAAAATTCTTATAAATCTGATTTCCTTTTTTTTAATCCAGCCAAAGAATCTGCTGAGATATTATGGTCTACGGTATATAACTTCGTCAATAGAAAATAGGGAATTGGCTTTGAAAACTGAGAAAACCCAAGAGTCAGATGAATCTGAAGAGGCTGAAAAGGACATAAACAATGACGTAGAGTGTGAAGAAGGGCAGAAAAAGGATGGAATTAGTGCATCTACTGCTTATGAGATTGCTGCCTCTGCTGCTTCTTATTTGCATTCTCATACCAGAAAAATACTACCATTCAGATCTTCTAAAACTGAGAATTCACTTGAAGCAAGTCAGAATAATGTTGATATGATGAACTCAGAGATGGCTTCTTTGATGGCAACCACAGATTCGGTTACCGCTGTCGTTGCTGCAAAGGAGGAAGTGAAGCAGGCGGTTGCAGACAATTTGAATTCAACTCGGTCGTCACCGTGCGAATGGTTTGTGTGCGACGACGTTGAGAGCAGTACAAGATTCTTTGTCATTCAGGTACACTTCACTTCCCTCTTGCCAAAAAAAGAACATATTAAAGAGTAGCTTAGGCATGATTACTGGAGGTTGCCATTTGATTGATTTGAAAGATCTTTTGTTCTATTTGGATTGGTCCTCGGAATGTTAATCTGATGCTAAAACAGTATTCTAAACATTAACAATCACCTGTTATCATTTGATGTATTTGACCTAATTTATATGGACTTTCTCTCATTGGTGCAGGGATCTGAATCACTAGCATCGTGGCAAGCAAATTTGCTTTTTGAACCAATCGATTTTGAGGTACAAATTCATGCTTCAAACTTTTTAAATCAATTGCTGTAGTTTGTTGCTTATTAGTTGCCATAAATTTTGAGATATGATGAATCTTTAAATATGCAACAAACTGATATATGTGTTTAAATGTCTTGTTATATAGGGACTAGGAGTCCTTGTCCACAGAGGAATCTATGAGGCTGCTAAAGGAATGTATGAACAGATGTTGCCTGATGTTCTCGAACACCTAAAATCCCATGGTGACCGCGCAACCTTCCGATTTACTGGACATTCTCTTGGGGGAAGTTTGGCGCTGCTAGTAAATCTCATGCTGTTGATAAGAAATGAGGTTCCGGTTTCGTCCTTGCTTCCTGTCATTACATTTGGTGCACCATCCATAATGTGTGGAGGCGACCGCTTACTTCGTAAACTTGGTTTGCCACGGAACCACCTTCAAGCTGTAACATTACATAGGGACATAGTACCACGAGCTTTCTCGTGCCAGTATCCGAACCATGTAGCAGAACTTCTTAAAGCCGTCAATGGGAACTTCAGGAATCATCCATGTCTAAGGAACCAGGTGAATTTCAAATGTTATACTGATCAAACAGATTTTTCTATGTTACAAATGCCTGATTAAGCGTCATAATCTAATATTGTGAACTATATGAACTGCAGAAATTGTTGTATGCTCCAATGGGCGAGTTTCTAATTCTTCAGCCCGATGAGAAATTCTCTCCAAGCCACGATCTCCTTCCTTCAGGCAGTGGTCTGTATCTTTTAACCTGTCCACTATCTGATGCCAATGATGGAGAGAAGCAGCTCCGAGCAGCACAGATGGTATTCTTAAACACGCCTCATCCACTCGAGACTCTTAGCGATCGATCCGCTTATGGCTCAGGTGGAACAATCCAAAGAGATCACGACATGAATTCATACCTAAAGTCAGTTCGAGGAGTGATTCGTCAAGAACTAAACCGTATAAGGAAAGCAAGGAGACAACATCGGCGCAAGGTATGGTGGGCTCTTGTGGCTCCAGGAAAAGTTGATATAGGAATTGTAATCGGACGGCCCGCCATTTCGATCAACCTTGGGCAGGACCAGTTCAACTTCTCCGGGATCCTGCAAACGGGAAGAGAGTCATTGAAACGGTTCAGTAGGCTTGTTGCTTCACAACATATGAATCTGCTTGTGGTGCTGTTGCTCCCTGCTAGATTGCTATTCTTTGAAGTCAACAGGGTGGTTGGCTAAAATTTAAAAGATAGAAGATTATGTTATTGTTGTTCTTGTTGTTGTTCATCTGGTTTCTTGTCACTTTTTTGGTGGAGCTGGATGAATGGCCTGTTTGTACAAATGGGGGTTCAATTGGTTGGCCAAACAGTTAGAAGA

mRNA sequence

ATGGAAGACAACCCAAATTCAAAGATCGTGGTGATCGTCGGAAGTATTCTATTGGATTTTGAGAAAACGAAAATAATGGATAGCCTCTGTTTGAATCCTGGAATACATGGGATTGCTTCGTCTTTATCAGTGAATGCGGCTCTTGATGTTCGTGTAAATCCGTCTCAGGTCAGTACGACCGGTAGGTCGTCGTCGTCGGCAGCGGTTGAGAAATCGTCGAAAACAATATCTCCGTCTCCGTCTCCGTCTCCGTCGCCATCGTCTTCGTCTTCATCGTTTCTGAAGTTTTCTTTGAAGTATCCATTGCAATCGCTGTGGAGTCGGAGTGGTGAAAGTGGGAATTCGAGGCGTAGTGGTTTGGCGCTTGACGACGCCGTTCTGATGGACAGTGAAGACGATCGGAGAGTCGTTTGTGAGGAGGAAAGTGAAAATGTAGCAACCGGATCGGACTGGAGAAATGCAAACTGGGTAATGAAGATTTTGCGGGTGAGATCTCTGTGGAGAGAGGAGGAGAAGCAGGGAATTGCAGAAGATGAGTTTAGAGAGGAGAGGGAGGAGGACCGTGTGGTGGAAGATCGAGAAATTTCTTGTGATGACGAGGAATTTTGTGATACTTGCGGAATCGTTGAGGAAGAAGATGAAAAGGAGGTTGAATTTGATAAACATTCGTTTTCGAGATTGCTTCGACGGGTTTCCTTGGCTGAAGCGAGGTTATATGCTCAAATGTCGTATTTAGGGTGCCTCGCATACTCCATTTCCGAAATTAAGCCAAAGAATCTGCTGAGATATTATGGTCTACGGTATATAACTTCGTCAATAGAAAATAGGGAATTGGCTTTGAAAACTGAGAAAACCCAAGAGTCAGATGAATCTGAAGAGGCTGAAAAGGACATAAACAATGACGTAGAGTGTGAAGAAGGGCAGAAAAAGGATGGAATTAGTGCATCTACTGCTTATGAGATTGCTGCCTCTGCTGCTTCTTATTTGCATTCTCATACCAGAAAAATACTACCATTCAGATCTTCTAAAACTGAGAATTCACTTGAAGCAAGTCAGAATAATGTTGATATGATGAACTCAGAGATGGCTTCTTTGATGGCAACCACAGATTCGGTTACCGCTGTCGTTGCTGCAAAGGAGGAAGTGAAGCAGGCGGTTGCAGACAATTTGAATTCAACTCGGTCGTCACCGTGCGAATGGTTTGTGTGCGACGACGTTGAGAGCAGTACAAGATTCTTTGTCATTCAGGGATCTGAATCACTAGCATCGTGGCAAGCAAATTTGCTTTTTGAACCAATCGATTTTGAGGGACTAGGAGTCCTTGTCCACAGAGGAATCTATGAGGCTGCTAAAGGAATGTATGAACAGATGTTGCCTGATGTTCTCGAACACCTAAAATCCCATGGTGACCGCGCAACCTTCCGATTTACTGGACATTCTCTTGGGGGAAGTTTGGCGCTGCTAGTAAATCTCATGCTGTTGATAAGAAATGAGGTTCCGGTTTCGTCCTTGCTTCCTGTCATTACATTTGGTGCACCATCCATAATGTGTGGAGGCGACCGCTTACTTCGTAAACTTGGTTTGCCACGGAACCACCTTCAAGCTGTAACATTACATAGGGACATAGTACCACGAGCTTTCTCGTGCCAGTATCCGAACCATGTAGCAGAACTTCTTAAAGCCGTCAATGGGAACTTCAGGAATCATCCATGTCTAAGGAACCAGAAATTGTTGTATGCTCCAATGGGCGAGTTTCTAATTCTTCAGCCCGATGAGAAATTCTCTCCAAGCCACGATCTCCTTCCTTCAGGCAGTGGTCTGTATCTTTTAACCTGTCCACTATCTGATGCCAATGATGGAGAGAAGCAGCTCCGAGCAGCACAGATGGTATTCTTAAACACGCCTCATCCACTCGAGACTCTTAGCGATCGATCCGCTTATGGCTCAGGTGGAACAATCCAAAGAGATCACGACATGAATTCATACCTAAAGTCAGTTCGAGGAGTGATTCGTCAAGAACTAAACCGTATAAGGAAAGCAAGGAGACAACATCGGCGCAAGGTATGGTGGGCTCTTGTGGCTCCAGGAAAAGTTGATATAGGAATTGTAATCGGACGGCCCGCCATTTCGATCAACCTTGGGCAGGACCAGTTCAACTTCTCCGGGATCCTGCAAACGGGAAGAGAGTCATTGAAACGGTTCAGTAGGCTTGTTGCTTCACAACATATGAATCTGCTTGTGGTGCTGTTGCTCCCTGCTAGATTGCTATTCTTTGAAGTCAACAGGGTGGTTGGCTAAAATTTAAAAGATAGAAGATTATGTTATTGTTGTTCTTGTTGTTGTTCATCTGGTTTCTTGTCACTTTTTTGGTGGAGCTGGATGAATGGCCTGTTTGTACAAATGGGGGTTCAATTGGTTGGCCAAACAGTTAGAAGA

Coding sequence (CDS)

ATGGAAGACAACCCAAATTCAAAGATCGTGGTGATCGTCGGAAGTATTCTATTGGATTTTGAGAAAACGAAAATAATGGATAGCCTCTGTTTGAATCCTGGAATACATGGGATTGCTTCGTCTTTATCAGTGAATGCGGCTCTTGATGTTCGTGTAAATCCGTCTCAGGTCAGTACGACCGGTAGGTCGTCGTCGTCGGCAGCGGTTGAGAAATCGTCGAAAACAATATCTCCGTCTCCGTCTCCGTCTCCGTCGCCATCGTCTTCGTCTTCATCGTTTCTGAAGTTTTCTTTGAAGTATCCATTGCAATCGCTGTGGAGTCGGAGTGGTGAAAGTGGGAATTCGAGGCGTAGTGGTTTGGCGCTTGACGACGCCGTTCTGATGGACAGTGAAGACGATCGGAGAGTCGTTTGTGAGGAGGAAAGTGAAAATGTAGCAACCGGATCGGACTGGAGAAATGCAAACTGGGTAATGAAGATTTTGCGGGTGAGATCTCTGTGGAGAGAGGAGGAGAAGCAGGGAATTGCAGAAGATGAGTTTAGAGAGGAGAGGGAGGAGGACCGTGTGGTGGAAGATCGAGAAATTTCTTGTGATGACGAGGAATTTTGTGATACTTGCGGAATCGTTGAGGAAGAAGATGAAAAGGAGGTTGAATTTGATAAACATTCGTTTTCGAGATTGCTTCGACGGGTTTCCTTGGCTGAAGCGAGGTTATATGCTCAAATGTCGTATTTAGGGTGCCTCGCATACTCCATTTCCGAAATTAAGCCAAAGAATCTGCTGAGATATTATGGTCTACGGTATATAACTTCGTCAATAGAAAATAGGGAATTGGCTTTGAAAACTGAGAAAACCCAAGAGTCAGATGAATCTGAAGAGGCTGAAAAGGACATAAACAATGACGTAGAGTGTGAAGAAGGGCAGAAAAAGGATGGAATTAGTGCATCTACTGCTTATGAGATTGCTGCCTCTGCTGCTTCTTATTTGCATTCTCATACCAGAAAAATACTACCATTCAGATCTTCTAAAACTGAGAATTCACTTGAAGCAAGTCAGAATAATGTTGATATGATGAACTCAGAGATGGCTTCTTTGATGGCAACCACAGATTCGGTTACCGCTGTCGTTGCTGCAAAGGAGGAAGTGAAGCAGGCGGTTGCAGACAATTTGAATTCAACTCGGTCGTCACCGTGCGAATGGTTTGTGTGCGACGACGTTGAGAGCAGTACAAGATTCTTTGTCATTCAGGGATCTGAATCACTAGCATCGTGGCAAGCAAATTTGCTTTTTGAACCAATCGATTTTGAGGGACTAGGAGTCCTTGTCCACAGAGGAATCTATGAGGCTGCTAAAGGAATGTATGAACAGATGTTGCCTGATGTTCTCGAACACCTAAAATCCCATGGTGACCGCGCAACCTTCCGATTTACTGGACATTCTCTTGGGGGAAGTTTGGCGCTGCTAGTAAATCTCATGCTGTTGATAAGAAATGAGGTTCCGGTTTCGTCCTTGCTTCCTGTCATTACATTTGGTGCACCATCCATAATGTGTGGAGGCGACCGCTTACTTCGTAAACTTGGTTTGCCACGGAACCACCTTCAAGCTGTAACATTACATAGGGACATAGTACCACGAGCTTTCTCGTGCCAGTATCCGAACCATGTAGCAGAACTTCTTAAAGCCGTCAATGGGAACTTCAGGAATCATCCATGTCTAAGGAACCAGAAATTGTTGTATGCTCCAATGGGCGAGTTTCTAATTCTTCAGCCCGATGAGAAATTCTCTCCAAGCCACGATCTCCTTCCTTCAGGCAGTGGTCTGTATCTTTTAACCTGTCCACTATCTGATGCCAATGATGGAGAGAAGCAGCTCCGAGCAGCACAGATGGTATTCTTAAACACGCCTCATCCACTCGAGACTCTTAGCGATCGATCCGCTTATGGCTCAGGTGGAACAATCCAAAGAGATCACGACATGAATTCATACCTAAAGTCAGTTCGAGGAGTGATTCGTCAAGAACTAAACCGTATAAGGAAAGCAAGGAGACAACATCGGCGCAAGGTATGGTGGGCTCTTGTGGCTCCAGGAAAAGTTGATATAGGAATTGTAATCGGACGGCCCGCCATTTCGATCAACCTTGGGCAGGACCAGTTCAACTTCTCCGGGATCCTGCAAACGGGAAGAGAGTCATTGAAACGGTTCAGTAGGCTTGTTGCTTCACAACATATGAATCTGCTTGTGGTGCTGTTGCTCCCTGCTAGATTGCTATTCTTTGAAGTCAACAGGGTGGTTGGCTAA

Protein sequence

MEDNPNSKIVVIVGSILLDFEKTKIMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSPSPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESENVATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCDTCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYYGLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAASAASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMNLLVVLLLPARLLFFEVNRVVG
Homology
BLAST of Lsi02G024620 vs. ExPASy Swiss-Prot
Match: F4HXL0 (Phospholipase A1 PLIP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP2 PE=1 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.7e-222
Identity = 436/744 (58.60%), Postives = 541/744 (72.72%), Query Frame = 0

Query: 26  MDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSPS 85
           MDSLCLN G+HG+  +               ++  G       VE  +   +PS    P 
Sbjct: 1   MDSLCLNSGLHGVIPA---------------ITAVGNGGCGGVVEVRATASAPSQKRGP- 60

Query: 86  PSSSSSSFLKFSLKYPLQSLWSRSGESG--NSRRSGLALDDAVLMDSEDDRRVVCEEESE 145
                     FS KYPL   WSR G  G  + RRSGL LDDAVL+DS D R+ + EE + 
Sbjct: 61  --------FGFSFKYPLTPFWSRGGGGGIASRRRSGLCLDDAVLVDSGDSRKPIAEETA- 120

Query: 146 NVATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFC 205
            V   ++ RN +WV+KIL V+S W+ EE++   E E  +  E++ V  D  +  +D+  C
Sbjct: 121 -VEMDTERRNGSWVLKILDVQSTWKHEEEEDDDEVEDEDGDEDEEVELDDAVVSEDDGGC 180

Query: 206 DTCGIVEEE--DEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLL 265
           D C ++E++  +  + + D+ SFS+LLRRV+L E++LYAQ+SYLG LAYSIS+IKP NL 
Sbjct: 181 DVCSVLEDDGNEANKFQLDRESFSKLLRRVTLPESKLYAQLSYLGNLAYSISKIKPANLS 240

Query: 266 RYYGLRYITSSIENRELALKTEKTQESDESE---EAEKDINNDVECEEGQKKDGISASTA 325
           +YYGLR++TSS E  E ALK E  + S E++   EAE+    +VE EE  K   ISAS A
Sbjct: 241 KYYGLRFVTSSAEKTESALKAENGEVSGETKPIVEAEE----EVEEEEKNKSRKISASAA 300

Query: 326 YEIAASAASYLHSHTRKILPFR-SSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVA 385
           YEI ASAASYLHS T  ILPF  SSK ENS    +++V++ N+E +S +A   SVT+VVA
Sbjct: 301 YEIVASAASYLHSRTNNILPFNSSSKAENS---DKHDVNLTNAESSSDVAY--SVTSVVA 360

Query: 386 AKEEVKQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEG 445
           A+E+VKQAVAD+L ST SSPC+WF+CDD +S TRF VIQGSESLASWQANLLFEPI+FEG
Sbjct: 361 AEEDVKQAVADDLKSTISSPCDWFICDDDQSHTRFVVIQGSESLASWQANLLFEPIEFEG 420

Query: 446 LGVLVHRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRN 505
           LG +VHRGIYEAAKGMYEQMLP+V  H+K+HG  A FRFTGHSLGGSL+LL+NLMLL+R 
Sbjct: 421 LGAIVHRGIYEAAKGMYEQMLPEVKAHIKTHGTSAKFRFTGHSLGGSLSLLLNLMLLVRG 480

Query: 506 EVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAE 565
           EVP SSLLPVIT+GAP ++CGGDRLL+KLGLP++H+QA+ +HRDIVPRAFSC YP HVAE
Sbjct: 481 EVPASSLLPVITYGAPFVLCGGDRLLKKLGLPKSHVQAIVMHRDIVPRAFSCNYPYHVAE 540

Query: 566 LLKAVNGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLS-- 625
           LLKAVNGNFR+HPCL  Q +LY+PMGE LILQPDE FSP H+LLPSG+GLYLLT      
Sbjct: 541 LLKAVNGNFRSHPCLNKQSMLYSPMGELLILQPDETFSPGHELLPSGNGLYLLTSDFESP 600

Query: 626 DANDG-EKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQEL 685
           D  D  E++LRAAQ VFLNTPHPL+ LSDRSAYGS GTIQRDHDMNSYLK+VR VIR+E+
Sbjct: 601 DIEDSDEERLRAAQTVFLNTPHPLDILSDRSAYGSSGTIQRDHDMNSYLKAVRSVIRKEV 660

Query: 686 NRIRKARRQHRRKVWW-ALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKR 745
           N+IR+A+R+HRR +WW  LVA      GI +    I+   GQD   FSG++QTGR+SL+R
Sbjct: 661 NQIRRAKREHRRSLWWPILVARESGSSGIAVSNGQIN---GQD---FSGMMQTGRKSLQR 703

Query: 746 FSRLVASQHMNLLVVLLLPARLLF 758
           FSRLVASQHM L+VV+L P +LLF
Sbjct: 721 FSRLVASQHMPLIVVMLFPVKLLF 703

BLAST of Lsi02G024620 vs. ExPASy Swiss-Prot
Match: Q940L4 (Phospholipase A1 PLIP3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP3 PE=1 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 5.0e-169
Identity = 342/644 (53.11%), Postives = 437/644 (67.86%), Query Frame = 0

Query: 123 DDAVLMDSEDDRRVVCEEESENVATGSDWRNANWVMKILRVRSLWR-EEEKQGIAEDEFR 182
           DDAVL++  D  R      +EN        N NWV+KIL V S+W+ + ++ G       
Sbjct: 71  DDAVLLERRDRNR------NEN-------DNGNWVLKILEVGSIWKGKRQRSGGGGGGEE 130

Query: 183 EEREEDRVVEDREISCDDEEFCDTCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQ 242
           +E EE    + +E  C++   CD C I ++++++E E     FS +L ++ + +A+++A+
Sbjct: 131 DEEEEVAEPKKKEDLCEE---CDFCRIDDDDEDEEKEKTVFEFSEMLSKIPVEDAQMFAK 190

Query: 243 MSYLGCLAYSISEIKPKNLLRYYGLRYITSSIENRELALKTEKTQESDESEEAEKDINND 302
           +S+LG LAYSI +IKP+NLL+Y  LR++TSSIE R ++LK E+    +E EE +K IN  
Sbjct: 191 LSFLGNLAYSIPKIKPENLLKYQKLRFVTSSIEKR-MSLKVEENNNGEEDEEKKKLIN-- 250

Query: 303 VECEEGQKKDGISASTAYEIAASAASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSE 362
                         + AY IAASAAS L SH++ +LPF SSK +             ++E
Sbjct: 251 -------------PAVAYRIAASAASRLFSHSKSVLPFGSSKRQ-------------DNE 310

Query: 363 MASLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESL 422
            ASL+AT DSVTAVVAAKEEVKQAVAD+L S RS PCEWFVCDD +S TRFF IQGS+SL
Sbjct: 311 EASLLATADSVTAVVAAKEEVKQAVADDLKSNRSPPCEWFVCDDDKSGTRFFFIQGSDSL 370

Query: 423 ASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSHG-DRATFRFTGHS 482
           ASWQANLLFEP+ FE L VLVHRGIYEAAKG+YEQMLP+V  HL S G +RA  RF+GHS
Sbjct: 371 ASWQANLLFEPVPFEDLDVLVHRGIYEAAKGIYEQMLPEVHAHLNSRGKNRAFLRFSGHS 430

Query: 483 LGGSLALLVNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHR 542
           LGGSL+LLVNLMLLIR +VP SSLLPVITFG+P IMCGGDRLL+KLGLP++HL  +++HR
Sbjct: 431 LGGSLSLLVNLMLLIRGQVPASSLLPVITFGSPCIMCGGDRLLQKLGLPKSHLLGISMHR 490

Query: 543 DIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDL 602
           DIVPRAFSC YPN  A+LLKA+NGNFRNHPCL NQ +LY+PMG+ LILQP E+FSP H L
Sbjct: 491 DIVPRAFSCNYPNRAAKLLKALNGNFRNHPCLNNQNVLYSPMGKLLILQPSERFSPPHPL 550

Query: 603 LPSGSGLYLLTCPLSDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMN 662
           LP GSGLYLL    +D  + EK LRAA+++F N+PHPLE LSDR +YGS G I+R+HDM+
Sbjct: 551 LPPGSGLYLLASKNTD--ETEKSLRAAKILFFNSPHPLEILSDRRSYGSEGKIKRNHDMS 610

Query: 663 SYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNF 722
           SYLK++R VIR+EL +++  R Q  RK                              F  
Sbjct: 611 SYLKALRHVIRKELKQMKAERDQWLRK------------------------------FFI 637

Query: 723 SGILQTGRESLKRFSRLVASQHMNLLVVLLLPARLLFFEVNRVV 765
             IL +GR+SLK  +R VAS+   L+++  LP RLL   V  VV
Sbjct: 671 INILFSGRDSLKLITRFVASRSSQLVIIFFLPIRLLIMSVYSVV 637

BLAST of Lsi02G024620 vs. ExPASy Swiss-Prot
Match: Q7Y220 (Phospholipase A1 PLIP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP1 PE=1 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 4.3e-128
Identity = 261/569 (45.87%), Postives = 349/569 (61.34%), Query Frame = 0

Query: 129 DSEDDRRVVCEEESENVATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDR 188
           D+  D +   E E E        + ANWV ++L +R  W+ E+K      +  EE     
Sbjct: 100 DTSQDEQESDEIEIETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDVAEES---- 159

Query: 189 VVEDREISCDDEEFCDTCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCL 248
              D    C++EE C     +        ++ + SFSRLL +VS +EA+  +Q++YL  L
Sbjct: 160 --VDVTCGCEEEEGC-----IANYGSVNGDWGRESFSRLLVKVSWSEAKKLSQLAYLCNL 219

Query: 249 AYSISEIKPKNLLRYYGLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQ 308
           AY+I EIK ++L R YGL+++TSS+E +  A    +  E D +       + D+E E+  
Sbjct: 220 AYTIPEIKGEDLRRNYGLKFVTSSLEKKAKAAILREKLEQDPT-HVPVITSPDLESEKQS 279

Query: 309 KKDGISASTAYEIAASAASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMAT 368
           ++   S+++AY+IAASAASY+HS     L                  + +    A+  A 
Sbjct: 280 QRSASSSASAYKIAASAASYIHSCKEYDLS-----------------EPIYKSAAAAQAA 339

Query: 369 TDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANL 428
             ++TAVVAA EE K   A  L S +SSPCEWFVCDD  + TR FVIQGS+SLASW+ANL
Sbjct: 340 ASTMTAVVAAGEEEKLEAARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANL 399

Query: 429 LFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALL 488
            FEP  FE   VLVHRGIYEAAKG+YEQ LP++ EHL  HGDRA F+FTGHSLGGSL+L+
Sbjct: 400 FFEPTKFEDTDVLVHRGIYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLI 459

Query: 489 VNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFS 548
           VNLML+ R  V   ++  V+TFG+P + CGG+++L +LGL  +H+  V +HRDIVPRAFS
Sbjct: 460 VNLMLISRGLVSSEAMKSVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFS 519

Query: 549 CQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLY 608
           C YP+HVA +LK +NG+FR HPCL   KLLY+PMG+  ILQP E  SP+H  LP G+ LY
Sbjct: 520 CNYPDHVALVLKRLNGSFRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALY 579

Query: 609 LLTCPLSDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRG 668
           +L       N  E     A   FLN PHPLETLS R+AYGS G++ RDHD  +Y+K+V G
Sbjct: 580 IL------ENSNEGYSPTALRAFLNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNG 633

Query: 669 VIRQELNRIRKARRQHRRKVWWALVAPGK 698
           V+RQ    I +  R  RR VW  L + G+
Sbjct: 640 VLRQHTKLIVRKARIQRRSVWPVLTSAGR 633

BLAST of Lsi02G024620 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.3e-04
Identity = 35/91 (38.46%), Postives = 48/91 (52.75%), Query Frame = 0

Query: 461 VLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPV-SSLLPVITFGAPSIMCGG 520
           +L+ L S  + A F  TGHSLGG+LA+L   +L++  E  +   LL V TFG P I   G
Sbjct: 320 ILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRI---G 379

Query: 521 DR-----LLRKLGLPRNHLQAVTLHRDIVPR 546
           +R     +  +L  P +    V    DIVPR
Sbjct: 380 NREVGLFMKAQLNQPVDRYFRVVYCNDIVPR 407

BLAST of Lsi02G024620 vs. ExPASy TrEMBL
Match: A0A1S3BRN4 (uncharacterized protein LOC103492768 OS=Cucumis melo OX=3656 GN=LOC103492768 PE=4 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 682/741 (92.04%), Postives = 705/741 (95.14%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQVST  RSSSS+  EKSSKTI  +PSPS 
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVSTAVRSSSSSVGEKSSKTI--TPSPSS 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
           S SSSSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++SEDDRRVV EE SEN
Sbjct: 61  SSSSSSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVESEDDRRVVREEGSEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLW+EEEKQGI EDE   EREEDRVVEDRE  C+DEEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWKEEEKQGIGEDELGNEREEDRVVEDRETCCEDEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 264
            C IVEEEDEKE+EFDKHSFSRLLRRVSLAE RLYAQMSYLGCLAYSISEIKPKNLLRYY
Sbjct: 181 ACKIVEEEDEKEIEFDKHSFSRLLRRVSLAETRLYAQMSYLGCLAYSISEIKPKNLLRYY 240

Query: 265 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAAS 324
           GLRYITSSIE RELALKTEKTQE DES EAEK+INNDV+CEEGQKKDGISASTAYEIAAS
Sbjct: 241 GLRYITSSIEKRELALKTEKTQEPDESREAEKEINNDVDCEEGQKKDGISASTAYEIAAS 300

Query: 325 AASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQ 384
           AASYLHS T KILPFRSSKTE+SLEA QNN DMMNS+M SLMATTDSVTAVVAAKEEVKQ
Sbjct: 301 AASYLHSRTVKILPFRSSKTEDSLEAGQNNGDMMNSDMVSLMATTDSVTAVVAAKEEVKQ 360

Query: 385 AVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 444
           AVADNLNSTRSSPCEW+VCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR
Sbjct: 361 AVADNLNSTRSSPCEWYVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 420

Query: 445 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 504
           GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL
Sbjct: 421 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 480

Query: 505 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 564
           LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG
Sbjct: 481 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 540

Query: 565 NFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQL 624
           NFRNHPCLRNQKLLYAPMGE LILQPDEKFSPSHDLLPSGSGLYLL CP SDAND EK+L
Sbjct: 541 NFRNHPCLRNQKLLYAPMGELLILQPDEKFSPSHDLLPSGSGLYLLNCPQSDANDAEKEL 600

Query: 625 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 684
           RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH
Sbjct: 601 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 660

Query: 685 RRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMN 744
           RRKVWWALVAPGKVD+GIV+GRP+ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHMN
Sbjct: 661 RRKVWWALVAPGKVDLGIVVGRPSISINLGQDQFNFSGILQTGRESLRRFSRLVASQHMN 720

Query: 745 LLVVLLLPARLLFFEVNRVVG 766
           LLVVLLLPARLLFFEVNRVVG
Sbjct: 721 LLVVLLLPARLLFFEVNRVVG 739

BLAST of Lsi02G024620 vs. ExPASy TrEMBL
Match: A0A5A7TTC6 (Lipase, class 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold427G00500 PE=4 SV=1)

HSP 1 Score: 1307.7 bits (3383), Expect = 0.0e+00
Identity = 680/741 (91.77%), Postives = 705/741 (95.14%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQV+T  RSSSS+ VEKSSKTI+PSPS   
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVTTAVRSSSSSVVEKSSKTITPSPS--- 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
             SSSSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++ EDD+RVV EEESEN
Sbjct: 61  --SSSSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVEREDDQRVVREEESEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLW+EEEKQGI EDE   EREEDRVVEDRE  C+DEEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWKEEEKQGIGEDELGNEREEDRVVEDRETCCEDEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 264
            C IVEEEDEKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY
Sbjct: 181 ACKIVEEEDEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 240

Query: 265 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAAS 324
           GLRYITSSIE RELALKTEKTQE DES EAEK+INNDV+CEEGQKKDGISASTAYEIAAS
Sbjct: 241 GLRYITSSIEKRELALKTEKTQEPDESREAEKEINNDVDCEEGQKKDGISASTAYEIAAS 300

Query: 325 AASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQ 384
           AASYLHS T KILPFRSSKTE+SLEA QNN DMMNS+M SLMATTDSVTAVVAAKEEVKQ
Sbjct: 301 AASYLHSRTVKILPFRSSKTEDSLEAGQNNGDMMNSDMVSLMATTDSVTAVVAAKEEVKQ 360

Query: 385 AVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 444
           AVADNLNSTRSSPCEW+VCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR
Sbjct: 361 AVADNLNSTRSSPCEWYVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 420

Query: 445 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 504
           GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL
Sbjct: 421 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 480

Query: 505 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 564
           L VITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG
Sbjct: 481 LSVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 540

Query: 565 NFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQL 624
           NFRNHPCLRNQKLLYAPMGE LILQPDEKFSPSHDLLPSGSGLYLL CP SDAND EK+L
Sbjct: 541 NFRNHPCLRNQKLLYAPMGELLILQPDEKFSPSHDLLPSGSGLYLLNCPQSDANDAEKEL 600

Query: 625 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 684
           RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH
Sbjct: 601 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 660

Query: 685 RRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMN 744
           RRKVWWALVAPGKVD+GIV+GRP+ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHMN
Sbjct: 661 RRKVWWALVAPGKVDLGIVVGRPSISINLGQDQFNFSGILQTGRESLRRFSRLVASQHMN 720

Query: 745 LLVVLLLPARLLFFEVNRVVG 766
           LLVVLLLPARLLFFEVNRVVG
Sbjct: 721 LLVVLLLPARLLFFEVNRVVG 736

BLAST of Lsi02G024620 vs. ExPASy TrEMBL
Match: A0A0A0K9H8 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407550 PE=4 SV=1)

HSP 1 Score: 1304.7 bits (3375), Expect = 0.0e+00
Identity = 683/751 (90.95%), Postives = 709/751 (94.41%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQVST  RSSSS+ VEKSSKTI  +PSPS 
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVSTAVRSSSSSVVEKSSKTI--TPSPSS 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
           S S+SSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++SE DRRVV EEESEN
Sbjct: 61  SSSASSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVESEVDRRVVPEEESEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLWRE+EKQG  EDE   EREEDRVVEDRE SCD+EEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWREDEKQGSGEDELGSEREEDRVVEDRETSCDEEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 264
           TC IVEEEDEKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY
Sbjct: 181 TCKIVEEEDEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 240

Query: 265 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAAS 324
           GLRYITSSIE RELALKTEKTQE DES+EAEKDINNDV+CEEGQKKDGISASTAYEIAAS
Sbjct: 241 GLRYITSSIEKRELALKTEKTQEPDESKEAEKDINNDVDCEEGQKKDGISASTAYEIAAS 300

Query: 325 AASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQ 384
           AASYLHS T KILPFRSSKTE+SLEASQNN DMMNS+M SLMATTDSVTAVVAAKEEVKQ
Sbjct: 301 AASYLHSRTIKILPFRSSKTEDSLEASQNNDDMMNSDMVSLMATTDSVTAVVAAKEEVKQ 360

Query: 385 AVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 444
           AVADNLNSTRSSPCEW+VCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR
Sbjct: 361 AVADNLNSTRSSPCEWYVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 420

Query: 445 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 504
           GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL
Sbjct: 421 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 480

Query: 505 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 564
           LPVITFGAPSIMCGGDRLL KLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG
Sbjct: 481 LPVITFGAPSIMCGGDRLLHKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 540

Query: 565 NFRNHPCLRNQ----------KLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPL 624
           NFRNHPCLRNQ          KLLYAPMGE LILQPDEKFSPSHDLLPSGSGLYLL+CP 
Sbjct: 541 NFRNHPCLRNQVSFKSYIDFSKLLYAPMGELLILQPDEKFSPSHDLLPSGSGLYLLSCPQ 600

Query: 625 SDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQEL 684
           SDAND EK+LRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQEL
Sbjct: 601 SDANDAEKELRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQEL 660

Query: 685 NRIRKARRQHRRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRF 744
           NRIRKARRQHRRKVWWALV+PGKVD+GIV+GRP ISINLGQDQFNFSGILQTGRESL+RF
Sbjct: 661 NRIRKARRQHRRKVWWALVSPGKVDLGIVVGRPTISINLGQDQFNFSGILQTGRESLRRF 720

Query: 745 SRLVASQHMNLLVVLLLPARLLFFEVNRVVG 766
           SRLVASQHMNLLVVLLLPAR+LFFEVNRVVG
Sbjct: 721 SRLVASQHMNLLVVLLLPARMLFFEVNRVVG 749

BLAST of Lsi02G024620 vs. ExPASy TrEMBL
Match: A0A5D3D3D9 (Lipase, class 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001570 PE=4 SV=1)

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 680/777 (87.52%), Postives = 705/777 (90.73%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQV+T  RSSSS+ VEKSSKTI+PSPS   
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVTTAVRSSSSSVVEKSSKTITPSPS--- 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
             SSSSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++ EDD+RVV EEESEN
Sbjct: 61  --SSSSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVEREDDQRVVREEESEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLW+EEEKQGI EDE   EREEDRVVEDRE  C+DEEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWKEEEKQGIGEDELGNEREEDRVVEDRETCCEDEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIK-------- 264
            C IVEEEDEKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIK        
Sbjct: 181 ACKIVEEEDEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKKIWVALDN 240

Query: 265 ----------------------------PKNLLRYYGLRYITSSIENRELALKTEKTQES 324
                                       PKNLLRYYGLRYITSSIE RELALKTEKTQE 
Sbjct: 241 RLLRSPVKIYNCSYKSDFFFFFXFWLIQPKNLLRYYGLRYITSSIEKRELALKTEKTQEP 300

Query: 325 DESEEAEKDINNDVECEEGQKKDGISASTAYEIAASAASYLHSHTRKILPFRSSKTENSL 384
           DES EAEK+INNDV+CEEGQKKDGISASTAYEIAASAASYLHS T KILPFRSSKTE+SL
Sbjct: 301 DESREAEKEINNDVDCEEGQKKDGISASTAYEIAASAASYLHSRTVKILPFRSSKTEDSL 360

Query: 385 EASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWFVCDDVES 444
           EA QNN DMMNS+M SLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEW+VCDDVES
Sbjct: 361 EAGQNNGDMMNSDMVSLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWYVCDDVES 420

Query: 445 STRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSH 504
           STRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSH
Sbjct: 421 STRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSH 480

Query: 505 GDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGL 564
           GDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLL VITFGAPSIMCGGDRLLRKLGL
Sbjct: 481 GDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLLSVITFGAPSIMCGGDRLLRKLGL 540

Query: 565 PRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGEFLIL 624
           PRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGE LIL
Sbjct: 541 PRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGELLIL 600

Query: 625 QPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYG 684
           QPDEKFSPSHDLLPSGSGLYLL CP SDAND EK+LRAAQMVFLNTPHPLETLSDRSAYG
Sbjct: 601 QPDEKFSPSHDLLPSGSGLYLLNCPQSDANDAEKELRAAQMVFLNTPHPLETLSDRSAYG 660

Query: 685 SGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVDIGIVIGRPA 744
           SGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVD+GIV+GRP+
Sbjct: 661 SGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVDLGIVVGRPS 720

Query: 745 ISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMNLLVVLLLPARLLFFEVNRVVG 766
           ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHMNLLVVLLLPARLLFFEVNRVVG
Sbjct: 721 ISINLGQDQFNFSGILQTGRESLRRFSRLVASQHMNLLVVLLLPARLLFFEVNRVVG 772

BLAST of Lsi02G024620 vs. ExPASy TrEMBL
Match: A0A6J1GN82 (uncharacterized protein LOC111455991 OS=Cucurbita moschata OX=3662 GN=LOC111455991 PE=4 SV=1)

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 659/743 (88.69%), Postives = 695/743 (93.54%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           +MDS CLNPGIHGIASSLSVNAALDVR NPS+VST GRSSS  AVEKS KTI    SPSP
Sbjct: 1   MMDSFCLNPGIHGIASSLSVNAALDVRGNPSKVSTAGRSSS--AVEKSQKTI----SPSP 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
           S +SSSSSFLKFSLKYPLQSLW+RSGE G+SRR GLALDDAVL++SED RR+V EEES N
Sbjct: 61  SSTSSSSSFLKFSLKYPLQSLWNRSGEGGDSRRGGLALDDAVLVESEDGRRIVPEEESRN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKIL VRSLWREE KQG +EDE R E ++DRV EDREISCD+EEFCD
Sbjct: 121 VATGSEWRSENWVMKILHVRSLWREEGKQGNSEDELRNEMDKDRVAEDREISCDEEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 264
           TC IVEEEDEKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLG LAYSISEIKPKNLLR+Y
Sbjct: 181 TCRIVEEEDEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGSLAYSISEIKPKNLLRHY 240

Query: 265 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDG--ISASTAYEIA 324
           G RY+TSSIE RELA+KTEKTQE  ES+EAEKD+NND + EE QKK+G  ISASTAY IA
Sbjct: 241 GQRYVTSSIEKRELAMKTEKTQELAESKEAEKDLNNDEQWEEVQKKNGHQISASTAYGIA 300

Query: 325 ASAASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEV 384
           ASAASYLHSHTRKILPFRS+KTE+SLEA+Q++VD MNS+MASLMATTDSVTAVVAAKEEV
Sbjct: 301 ASAASYLHSHTRKILPFRSTKTEDSLEANQDDVDKMNSDMASLMATTDSVTAVVAAKEEV 360

Query: 385 KQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLV 444
           KQAVADNLNSTRSSPCEWFVCDD ESSTRFFVIQGSESLASWQANLLFEP+DFEGLGVLV
Sbjct: 361 KQAVADNLNSTRSSPCEWFVCDDDESSTRFFVIQGSESLASWQANLLFEPVDFEGLGVLV 420

Query: 445 HRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVS 504
           HRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVS
Sbjct: 421 HRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVS 480

Query: 505 SLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAV 564
           SLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAV
Sbjct: 481 SLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAV 540

Query: 565 NGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEK 624
           NGNFRNHPCL NQKLLYAPMGE +ILQPDEKFSPSHDLLPSGSGLYLL CP SDAND EK
Sbjct: 541 NGNFRNHPCLSNQKLLYAPMGELVILQPDEKFSPSHDLLPSGSGLYLLRCPQSDANDAEK 600

Query: 625 QLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARR 684
           QL+AAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDM+SYLKSVRGVIRQELNRIRKARR
Sbjct: 601 QLQAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMSSYLKSVRGVIRQELNRIRKARR 660

Query: 685 QHRRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQH 744
           +HRRKVWWAL+APGKVDIGIVIGRP ISINLGQDQF FSGILQTGRES +RFSRLVASQH
Sbjct: 661 EHRRKVWWALMAPGKVDIGIVIGRPTISINLGQDQFKFSGILQTGRESFQRFSRLVASQH 720

Query: 745 MNLLVVLLLPARLLFFEVNRVVG 766
           MNLLV+LLLPARLL FE NRVVG
Sbjct: 721 MNLLVLLLLPARLLLFEANRVVG 737

BLAST of Lsi02G024620 vs. NCBI nr
Match: XP_038899170.1 (phospholipase A1 PLIP2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 692/742 (93.26%), Postives = 714/742 (96.23%), Query Frame = 0

Query: 26  MDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSPS 85
           MDS CLNPGIHGIASSLSVN ALDVRVNPSQVSTTGRSS++AAVEKSSKTIS   S S S
Sbjct: 1   MDSFCLNPGIHGIASSLSVNGALDVRVNPSQVSTTGRSSAAAAVEKSSKTIS---SSSSS 60

Query: 86  PSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESENV 145
            SSSSSSFLKFSLKYPLQSLWSRSGE+GNSRR GLALDDAVL++SEDDRRVVCEEE++NV
Sbjct: 61  SSSSSSSFLKFSLKYPLQSLWSRSGENGNSRRGGLALDDAVLVESEDDRRVVCEEENKNV 120

Query: 146 ATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREERE-EDRVVEDREISCDDEEFCD 205
           ATGS+WR+ NWVMKIL+VRSLWREEEKQGI EDE R+ERE ED VVEDREISCDD+EFCD
Sbjct: 121 ATGSEWRSGNWVMKILQVRSLWREEEKQGIEEDELRKEREKEDHVVEDREISCDDDEFCD 180

Query: 206 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 265
           TC IVEEE+EKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY
Sbjct: 181 TCRIVEEENEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 240

Query: 266 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAAS 325
           GLRYITSSIE REL LKTEKTQE DES+E EKDINN+VECEEGQKKDGISASTAYEIAAS
Sbjct: 241 GLRYITSSIERRELTLKTEKTQEPDESKEVEKDINNNVECEEGQKKDGISASTAYEIAAS 300

Query: 326 AASYLHSHTRKILPFRSSKTENSL-EASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVK 385
           AASYLHSHTRKILPFRSSKTE+SL E +QNNVDMM+SEMASLMATTDSVTAVVAAKEEVK
Sbjct: 301 AASYLHSHTRKILPFRSSKTEDSLEEENQNNVDMMSSEMASLMATTDSVTAVVAAKEEVK 360

Query: 386 QAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVH 445
           QAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVH
Sbjct: 361 QAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVH 420

Query: 446 RGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSS 505
           RGIYEAAKGMYEQMLPDVLEHLKSHGDRA FRFTGHSLGGSLALLVNLMLLIRNEVPVSS
Sbjct: 421 RGIYEAAKGMYEQMLPDVLEHLKSHGDRANFRFTGHSLGGSLALLVNLMLLIRNEVPVSS 480

Query: 506 LLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVN 565
           LLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVN
Sbjct: 481 LLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVN 540

Query: 566 GNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQ 625
           GNFRNHPCL NQKLLYAPMGE LILQPDEKFSPSHDLLPSGSGLYLL+CP SD +D EKQ
Sbjct: 541 GNFRNHPCLMNQKLLYAPMGELLILQPDEKFSPSHDLLPSGSGLYLLSCPQSDVSDAEKQ 600

Query: 626 LRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQ 685
           LRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQ
Sbjct: 601 LRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQ 660

Query: 686 HRRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQHM 745
           HRRKVWWALVAPGKVDIGIVIGRP ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHM
Sbjct: 661 HRRKVWWALVAPGKVDIGIVIGRPTISINLGQDQFNFSGILQTGRESLRRFSRLVASQHM 720

Query: 746 NLLVVLLLPARLLFFEVNRVVG 766
           NLLVVLLLPARLLFFEVNRVVG
Sbjct: 721 NLLVVLLLPARLLFFEVNRVVG 739

BLAST of Lsi02G024620 vs. NCBI nr
Match: XP_011659388.1 (phospholipase A1 PLIP2, chloroplastic [Cucumis sativus] >KAE8646362.1 hypothetical protein Csa_016469 [Cucumis sativus])

HSP 1 Score: 1312.7 bits (3396), Expect = 0.0e+00
Identity = 683/741 (92.17%), Postives = 709/741 (95.68%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQVST  RSSSS+ VEKSSKTI  +PSPS 
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVSTAVRSSSSSVVEKSSKTI--TPSPSS 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
           S S+SSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++SE DRRVV EEESEN
Sbjct: 61  SSSASSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVESEVDRRVVPEEESEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLWRE+EKQG  EDE   EREEDRVVEDRE SCD+EEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWREDEKQGSGEDELGSEREEDRVVEDRETSCDEEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 264
           TC IVEEEDEKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY
Sbjct: 181 TCKIVEEEDEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 240

Query: 265 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAAS 324
           GLRYITSSIE RELALKTEKTQE DES+EAEKDINNDV+CEEGQKKDGISASTAYEIAAS
Sbjct: 241 GLRYITSSIEKRELALKTEKTQEPDESKEAEKDINNDVDCEEGQKKDGISASTAYEIAAS 300

Query: 325 AASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQ 384
           AASYLHS T KILPFRSSKTE+SLEASQNN DMMNS+M SLMATTDSVTAVVAAKEEVKQ
Sbjct: 301 AASYLHSRTIKILPFRSSKTEDSLEASQNNDDMMNSDMVSLMATTDSVTAVVAAKEEVKQ 360

Query: 385 AVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 444
           AVADNLNSTRSSPCEW+VCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR
Sbjct: 361 AVADNLNSTRSSPCEWYVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 420

Query: 445 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 504
           GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL
Sbjct: 421 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 480

Query: 505 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 564
           LPVITFGAPSIMCGGDRLL KLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG
Sbjct: 481 LPVITFGAPSIMCGGDRLLHKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 540

Query: 565 NFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQL 624
           NFRNHPCLRNQKLLYAPMGE LILQPDEKFSPSHDLLPSGSGLYLL+CP SDAND EK+L
Sbjct: 541 NFRNHPCLRNQKLLYAPMGELLILQPDEKFSPSHDLLPSGSGLYLLSCPQSDANDAEKEL 600

Query: 625 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 684
           RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH
Sbjct: 601 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 660

Query: 685 RRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMN 744
           RRKVWWALV+PGKVD+GIV+GRP ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHMN
Sbjct: 661 RRKVWWALVSPGKVDLGIVVGRPTISINLGQDQFNFSGILQTGRESLRRFSRLVASQHMN 720

Query: 745 LLVVLLLPARLLFFEVNRVVG 766
           LLVVLLLPAR+LFFEVNRVVG
Sbjct: 721 LLVVLLLPARMLFFEVNRVVG 739

BLAST of Lsi02G024620 vs. NCBI nr
Match: XP_008451496.1 (PREDICTED: uncharacterized protein LOC103492768 [Cucumis melo])

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 682/741 (92.04%), Postives = 705/741 (95.14%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQVST  RSSSS+  EKSSKTI  +PSPS 
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVSTAVRSSSSSVGEKSSKTI--TPSPSS 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
           S SSSSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++SEDDRRVV EE SEN
Sbjct: 61  SSSSSSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVESEDDRRVVREEGSEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLW+EEEKQGI EDE   EREEDRVVEDRE  C+DEEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWKEEEKQGIGEDELGNEREEDRVVEDRETCCEDEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 264
            C IVEEEDEKE+EFDKHSFSRLLRRVSLAE RLYAQMSYLGCLAYSISEIKPKNLLRYY
Sbjct: 181 ACKIVEEEDEKEIEFDKHSFSRLLRRVSLAETRLYAQMSYLGCLAYSISEIKPKNLLRYY 240

Query: 265 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAAS 324
           GLRYITSSIE RELALKTEKTQE DES EAEK+INNDV+CEEGQKKDGISASTAYEIAAS
Sbjct: 241 GLRYITSSIEKRELALKTEKTQEPDESREAEKEINNDVDCEEGQKKDGISASTAYEIAAS 300

Query: 325 AASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQ 384
           AASYLHS T KILPFRSSKTE+SLEA QNN DMMNS+M SLMATTDSVTAVVAAKEEVKQ
Sbjct: 301 AASYLHSRTVKILPFRSSKTEDSLEAGQNNGDMMNSDMVSLMATTDSVTAVVAAKEEVKQ 360

Query: 385 AVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 444
           AVADNLNSTRSSPCEW+VCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR
Sbjct: 361 AVADNLNSTRSSPCEWYVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 420

Query: 445 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 504
           GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL
Sbjct: 421 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 480

Query: 505 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 564
           LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG
Sbjct: 481 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 540

Query: 565 NFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQL 624
           NFRNHPCLRNQKLLYAPMGE LILQPDEKFSPSHDLLPSGSGLYLL CP SDAND EK+L
Sbjct: 541 NFRNHPCLRNQKLLYAPMGELLILQPDEKFSPSHDLLPSGSGLYLLNCPQSDANDAEKEL 600

Query: 625 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 684
           RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH
Sbjct: 601 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 660

Query: 685 RRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMN 744
           RRKVWWALVAPGKVD+GIV+GRP+ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHMN
Sbjct: 661 RRKVWWALVAPGKVDLGIVVGRPSISINLGQDQFNFSGILQTGRESLRRFSRLVASQHMN 720

Query: 745 LLVVLLLPARLLFFEVNRVVG 766
           LLVVLLLPARLLFFEVNRVVG
Sbjct: 721 LLVVLLLPARLLFFEVNRVVG 739

BLAST of Lsi02G024620 vs. NCBI nr
Match: KAA0046682.1 (Lipase, class 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1307.7 bits (3383), Expect = 0.0e+00
Identity = 680/741 (91.77%), Postives = 705/741 (95.14%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQV+T  RSSSS+ VEKSSKTI+PSPS   
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVTTAVRSSSSSVVEKSSKTITPSPS--- 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
             SSSSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++ EDD+RVV EEESEN
Sbjct: 61  --SSSSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVEREDDQRVVREEESEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLW+EEEKQGI EDE   EREEDRVVEDRE  C+DEEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWKEEEKQGIGEDELGNEREEDRVVEDRETCCEDEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 264
            C IVEEEDEKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY
Sbjct: 181 ACKIVEEEDEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLLRYY 240

Query: 265 GLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQKKDGISASTAYEIAAS 324
           GLRYITSSIE RELALKTEKTQE DES EAEK+INNDV+CEEGQKKDGISASTAYEIAAS
Sbjct: 241 GLRYITSSIEKRELALKTEKTQEPDESREAEKEINNDVDCEEGQKKDGISASTAYEIAAS 300

Query: 325 AASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQ 384
           AASYLHS T KILPFRSSKTE+SLEA QNN DMMNS+M SLMATTDSVTAVVAAKEEVKQ
Sbjct: 301 AASYLHSRTVKILPFRSSKTEDSLEAGQNNGDMMNSDMVSLMATTDSVTAVVAAKEEVKQ 360

Query: 385 AVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 444
           AVADNLNSTRSSPCEW+VCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR
Sbjct: 361 AVADNLNSTRSSPCEWYVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHR 420

Query: 445 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 504
           GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL
Sbjct: 421 GIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSL 480

Query: 505 LPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 564
           L VITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG
Sbjct: 481 LSVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNG 540

Query: 565 NFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQL 624
           NFRNHPCLRNQKLLYAPMGE LILQPDEKFSPSHDLLPSGSGLYLL CP SDAND EK+L
Sbjct: 541 NFRNHPCLRNQKLLYAPMGELLILQPDEKFSPSHDLLPSGSGLYLLNCPQSDANDAEKEL 600

Query: 625 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 684
           RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH
Sbjct: 601 RAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQH 660

Query: 685 RRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMN 744
           RRKVWWALVAPGKVD+GIV+GRP+ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHMN
Sbjct: 661 RRKVWWALVAPGKVDLGIVVGRPSISINLGQDQFNFSGILQTGRESLRRFSRLVASQHMN 720

Query: 745 LLVVLLLPARLLFFEVNRVVG 766
           LLVVLLLPARLLFFEVNRVVG
Sbjct: 721 LLVVLLLPARLLFFEVNRVVG 736

BLAST of Lsi02G024620 vs. NCBI nr
Match: TYK18218.1 (Lipase, class 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 680/777 (87.52%), Postives = 705/777 (90.73%), Query Frame = 0

Query: 25  IMDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSP 84
           ++DS CLNPGIHGI SSLS+NAALDVRVNPSQV+T  RSSSS+ VEKSSKTI+PSPS   
Sbjct: 1   MIDSFCLNPGIHGITSSLSLNAALDVRVNPSQVTTAVRSSSSSVVEKSSKTITPSPS--- 60

Query: 85  SPSSSSSSFLKFSLKYPLQSLWSRSGESGNSRRSGLALDDAVLMDSEDDRRVVCEEESEN 144
             SSSSSSFLKFSLKYPLQSLWSR GE+GNSRR GLALDDAVL++ EDD+RVV EEESEN
Sbjct: 61  --SSSSSSFLKFSLKYPLQSLWSRGGENGNSRRGGLALDDAVLVEREDDQRVVREEESEN 120

Query: 145 VATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFCD 204
           VATGS+WR+ NWVMKILRVRSLW+EEEKQGI EDE   EREEDRVVEDRE  C+DEEFCD
Sbjct: 121 VATGSEWRSGNWVMKILRVRSLWKEEEKQGIGEDELGNEREEDRVVEDRETCCEDEEFCD 180

Query: 205 TCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIK-------- 264
            C IVEEEDEKE+EFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIK        
Sbjct: 181 ACKIVEEEDEKEIEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKKIWVALDN 240

Query: 265 ----------------------------PKNLLRYYGLRYITSSIENRELALKTEKTQES 324
                                       PKNLLRYYGLRYITSSIE RELALKTEKTQE 
Sbjct: 241 RLLRSPVKIYNCSYKSDFFFFFXFWLIQPKNLLRYYGLRYITSSIEKRELALKTEKTQEP 300

Query: 325 DESEEAEKDINNDVECEEGQKKDGISASTAYEIAASAASYLHSHTRKILPFRSSKTENSL 384
           DES EAEK+INNDV+CEEGQKKDGISASTAYEIAASAASYLHS T KILPFRSSKTE+SL
Sbjct: 301 DESREAEKEINNDVDCEEGQKKDGISASTAYEIAASAASYLHSRTVKILPFRSSKTEDSL 360

Query: 385 EASQNNVDMMNSEMASLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWFVCDDVES 444
           EA QNN DMMNS+M SLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEW+VCDDVES
Sbjct: 361 EAGQNNGDMMNSDMVSLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWYVCDDVES 420

Query: 445 STRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSH 504
           STRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSH
Sbjct: 421 STRFFVIQGSESLASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSH 480

Query: 505 GDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGL 564
           GDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLL VITFGAPSIMCGGDRLLRKLGL
Sbjct: 481 GDRATFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLLSVITFGAPSIMCGGDRLLRKLGL 540

Query: 565 PRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGEFLIL 624
           PRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGE LIL
Sbjct: 541 PRNHLQAVTLHRDIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGELLIL 600

Query: 625 QPDEKFSPSHDLLPSGSGLYLLTCPLSDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYG 684
           QPDEKFSPSHDLLPSGSGLYLL CP SDAND EK+LRAAQMVFLNTPHPLETLSDRSAYG
Sbjct: 601 QPDEKFSPSHDLLPSGSGLYLLNCPQSDANDAEKELRAAQMVFLNTPHPLETLSDRSAYG 660

Query: 685 SGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVDIGIVIGRPA 744
           SGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVD+GIV+GRP+
Sbjct: 661 SGGTIQRDHDMNSYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVDLGIVVGRPS 720

Query: 745 ISINLGQDQFNFSGILQTGRESLKRFSRLVASQHMNLLVVLLLPARLLFFEVNRVVG 766
           ISINLGQDQFNFSGILQTGRESL+RFSRLVASQHMNLLVVLLLPARLLFFEVNRVVG
Sbjct: 721 ISINLGQDQFNFSGILQTGRESLRRFSRLVASQHMNLLVVLLLPARLLFFEVNRVVG 772

BLAST of Lsi02G024620 vs. TAIR 10
Match: AT1G02660.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 773.9 bits (1997), Expect = 1.2e-223
Identity = 436/744 (58.60%), Postives = 541/744 (72.72%), Query Frame = 0

Query: 26  MDSLCLNPGIHGIASSLSVNAALDVRVNPSQVSTTGRSSSSAAVEKSSKTISPSPSPSPS 85
           MDSLCLN G+HG+  +               ++  G       VE  +   +PS    P 
Sbjct: 1   MDSLCLNSGLHGVIPA---------------ITAVGNGGCGGVVEVRATASAPSQKRGP- 60

Query: 86  PSSSSSSFLKFSLKYPLQSLWSRSGESG--NSRRSGLALDDAVLMDSEDDRRVVCEEESE 145
                     FS KYPL   WSR G  G  + RRSGL LDDAVL+DS D R+ + EE + 
Sbjct: 61  --------FGFSFKYPLTPFWSRGGGGGIASRRRSGLCLDDAVLVDSGDSRKPIAEETA- 120

Query: 146 NVATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDRVVEDREISCDDEEFC 205
            V   ++ RN +WV+KIL V+S W+ EE++   E E  +  E++ V  D  +  +D+  C
Sbjct: 121 -VEMDTERRNGSWVLKILDVQSTWKHEEEEDDDEVEDEDGDEDEEVELDDAVVSEDDGGC 180

Query: 206 DTCGIVEEE--DEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCLAYSISEIKPKNLL 265
           D C ++E++  +  + + D+ SFS+LLRRV+L E++LYAQ+SYLG LAYSIS+IKP NL 
Sbjct: 181 DVCSVLEDDGNEANKFQLDRESFSKLLRRVTLPESKLYAQLSYLGNLAYSISKIKPANLS 240

Query: 266 RYYGLRYITSSIENRELALKTEKTQESDESE---EAEKDINNDVECEEGQKKDGISASTA 325
           +YYGLR++TSS E  E ALK E  + S E++   EAE+    +VE EE  K   ISAS A
Sbjct: 241 KYYGLRFVTSSAEKTESALKAENGEVSGETKPIVEAEE----EVEEEEKNKSRKISASAA 300

Query: 326 YEIAASAASYLHSHTRKILPFR-SSKTENSLEASQNNVDMMNSEMASLMATTDSVTAVVA 385
           YEI ASAASYLHS T  ILPF  SSK ENS    +++V++ N+E +S +A   SVT+VVA
Sbjct: 301 YEIVASAASYLHSRTNNILPFNSSSKAENS---DKHDVNLTNAESSSDVAY--SVTSVVA 360

Query: 386 AKEEVKQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANLLFEPIDFEG 445
           A+E+VKQAVAD+L ST SSPC+WF+CDD +S TRF VIQGSESLASWQANLLFEPI+FEG
Sbjct: 361 AEEDVKQAVADDLKSTISSPCDWFICDDDQSHTRFVVIQGSESLASWQANLLFEPIEFEG 420

Query: 446 LGVLVHRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRN 505
           LG +VHRGIYEAAKGMYEQMLP+V  H+K+HG  A FRFTGHSLGGSL+LL+NLMLL+R 
Sbjct: 421 LGAIVHRGIYEAAKGMYEQMLPEVKAHIKTHGTSAKFRFTGHSLGGSLSLLLNLMLLVRG 480

Query: 506 EVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFSCQYPNHVAE 565
           EVP SSLLPVIT+GAP ++CGGDRLL+KLGLP++H+QA+ +HRDIVPRAFSC YP HVAE
Sbjct: 481 EVPASSLLPVITYGAPFVLCGGDRLLKKLGLPKSHVQAIVMHRDIVPRAFSCNYPYHVAE 540

Query: 566 LLKAVNGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLYLLTCPLS-- 625
           LLKAVNGNFR+HPCL  Q +LY+PMGE LILQPDE FSP H+LLPSG+GLYLLT      
Sbjct: 541 LLKAVNGNFRSHPCLNKQSMLYSPMGELLILQPDETFSPGHELLPSGNGLYLLTSDFESP 600

Query: 626 DANDG-EKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRGVIRQEL 685
           D  D  E++LRAAQ VFLNTPHPL+ LSDRSAYGS GTIQRDHDMNSYLK+VR VIR+E+
Sbjct: 601 DIEDSDEERLRAAQTVFLNTPHPLDILSDRSAYGSSGTIQRDHDMNSYLKAVRSVIRKEV 660

Query: 686 NRIRKARRQHRRKVWW-ALVAPGKVDIGIVIGRPAISINLGQDQFNFSGILQTGRESLKR 745
           N+IR+A+R+HRR +WW  LVA      GI +    I+   GQD   FSG++QTGR+SL+R
Sbjct: 661 NQIRRAKREHRRSLWWPILVARESGSSGIAVSNGQIN---GQD---FSGMMQTGRKSLQR 703

Query: 746 FSRLVASQHMNLLVVLLLPARLLF 758
           FSRLVASQHM L+VV+L P +LLF
Sbjct: 721 FSRLVASQHMPLIVVMLFPVKLLF 703

BLAST of Lsi02G024620 vs. TAIR 10
Match: AT3G62590.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 596.3 bits (1536), Expect = 3.5e-170
Identity = 342/644 (53.11%), Postives = 437/644 (67.86%), Query Frame = 0

Query: 123 DDAVLMDSEDDRRVVCEEESENVATGSDWRNANWVMKILRVRSLWR-EEEKQGIAEDEFR 182
           DDAVL++  D  R      +EN        N NWV+KIL V S+W+ + ++ G       
Sbjct: 71  DDAVLLERRDRNR------NEN-------DNGNWVLKILEVGSIWKGKRQRSGGGGGGEE 130

Query: 183 EEREEDRVVEDREISCDDEEFCDTCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQ 242
           +E EE    + +E  C++   CD C I ++++++E E     FS +L ++ + +A+++A+
Sbjct: 131 DEEEEVAEPKKKEDLCEE---CDFCRIDDDDEDEEKEKTVFEFSEMLSKIPVEDAQMFAK 190

Query: 243 MSYLGCLAYSISEIKPKNLLRYYGLRYITSSIENRELALKTEKTQESDESEEAEKDINND 302
           +S+LG LAYSI +IKP+NLL+Y  LR++TSSIE R ++LK E+    +E EE +K IN  
Sbjct: 191 LSFLGNLAYSIPKIKPENLLKYQKLRFVTSSIEKR-MSLKVEENNNGEEDEEKKKLIN-- 250

Query: 303 VECEEGQKKDGISASTAYEIAASAASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSE 362
                         + AY IAASAAS L SH++ +LPF SSK +             ++E
Sbjct: 251 -------------PAVAYRIAASAASRLFSHSKSVLPFGSSKRQ-------------DNE 310

Query: 363 MASLMATTDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESL 422
            ASL+AT DSVTAVVAAKEEVKQAVAD+L S RS PCEWFVCDD +S TRFF IQGS+SL
Sbjct: 311 EASLLATADSVTAVVAAKEEVKQAVADDLKSNRSPPCEWFVCDDDKSGTRFFFIQGSDSL 370

Query: 423 ASWQANLLFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSHG-DRATFRFTGHS 482
           ASWQANLLFEP+ FE L VLVHRGIYEAAKG+YEQMLP+V  HL S G +RA  RF+GHS
Sbjct: 371 ASWQANLLFEPVPFEDLDVLVHRGIYEAAKGIYEQMLPEVHAHLNSRGKNRAFLRFSGHS 430

Query: 483 LGGSLALLVNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHR 542
           LGGSL+LLVNLMLLIR +VP SSLLPVITFG+P IMCGGDRLL+KLGLP++HL  +++HR
Sbjct: 431 LGGSLSLLVNLMLLIRGQVPASSLLPVITFGSPCIMCGGDRLLQKLGLPKSHLLGISMHR 490

Query: 543 DIVPRAFSCQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDL 602
           DIVPRAFSC YPN  A+LLKA+NGNFRNHPCL NQ +LY+PMG+ LILQP E+FSP H L
Sbjct: 491 DIVPRAFSCNYPNRAAKLLKALNGNFRNHPCLNNQNVLYSPMGKLLILQPSERFSPPHPL 550

Query: 603 LPSGSGLYLLTCPLSDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMN 662
           LP GSGLYLL    +D  + EK LRAA+++F N+PHPLE LSDR +YGS G I+R+HDM+
Sbjct: 551 LPPGSGLYLLASKNTD--ETEKSLRAAKILFFNSPHPLEILSDRRSYGSEGKIKRNHDMS 610

Query: 663 SYLKSVRGVIRQELNRIRKARRQHRRKVWWALVAPGKVDIGIVIGRPAISINLGQDQFNF 722
           SYLK++R VIR+EL +++  R Q  RK                              F  
Sbjct: 611 SYLKALRHVIRKELKQMKAERDQWLRK------------------------------FFI 637

Query: 723 SGILQTGRESLKRFSRLVASQHMNLLVVLLLPARLLFFEVNRVV 765
             IL +GR+SLK  +R VAS+   L+++  LP RLL   V  VV
Sbjct: 671 INILFSGRDSLKLITRFVASRSSQLVIIFFLPIRLLIMSVYSVV 637

BLAST of Lsi02G024620 vs. TAIR 10
Match: AT3G61680.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 460.3 bits (1183), Expect = 3.0e-129
Identity = 261/569 (45.87%), Postives = 349/569 (61.34%), Query Frame = 0

Query: 129 DSEDDRRVVCEEESENVATGSDWRNANWVMKILRVRSLWREEEKQGIAEDEFREEREEDR 188
           D+  D +   E E E        + ANWV ++L +R  W+ E+K      +  EE     
Sbjct: 100 DTSQDEQESDEIEIETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDVAEES---- 159

Query: 189 VVEDREISCDDEEFCDTCGIVEEEDEKEVEFDKHSFSRLLRRVSLAEARLYAQMSYLGCL 248
              D    C++EE C     +        ++ + SFSRLL +VS +EA+  +Q++YL  L
Sbjct: 160 --VDVTCGCEEEEGC-----IANYGSVNGDWGRESFSRLLVKVSWSEAKKLSQLAYLCNL 219

Query: 249 AYSISEIKPKNLLRYYGLRYITSSIENRELALKTEKTQESDESEEAEKDINNDVECEEGQ 308
           AY+I EIK ++L R YGL+++TSS+E +  A    +  E D +       + D+E E+  
Sbjct: 220 AYTIPEIKGEDLRRNYGLKFVTSSLEKKAKAAILREKLEQDPT-HVPVITSPDLESEKQS 279

Query: 309 KKDGISASTAYEIAASAASYLHSHTRKILPFRSSKTENSLEASQNNVDMMNSEMASLMAT 368
           ++   S+++AY+IAASAASY+HS     L                  + +    A+  A 
Sbjct: 280 QRSASSSASAYKIAASAASYIHSCKEYDLS-----------------EPIYKSAAAAQAA 339

Query: 369 TDSVTAVVAAKEEVKQAVADNLNSTRSSPCEWFVCDDVESSTRFFVIQGSESLASWQANL 428
             ++TAVVAA EE K   A  L S +SSPCEWFVCDD  + TR FVIQGS+SLASW+ANL
Sbjct: 340 ASTMTAVVAAGEEEKLEAARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANL 399

Query: 429 LFEPIDFEGLGVLVHRGIYEAAKGMYEQMLPDVLEHLKSHGDRATFRFTGHSLGGSLALL 488
            FEP  FE   VLVHRGIYEAAKG+YEQ LP++ EHL  HGDRA F+FTGHSLGGSL+L+
Sbjct: 400 FFEPTKFEDTDVLVHRGIYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLI 459

Query: 489 VNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNHLQAVTLHRDIVPRAFS 548
           VNLML+ R  V   ++  V+TFG+P + CGG+++L +LGL  +H+  V +HRDIVPRAFS
Sbjct: 460 VNLMLISRGLVSSEAMKSVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFS 519

Query: 549 CQYPNHVAELLKAVNGNFRNHPCLRNQKLLYAPMGEFLILQPDEKFSPSHDLLPSGSGLY 608
           C YP+HVA +LK +NG+FR HPCL   KLLY+PMG+  ILQP E  SP+H  LP G+ LY
Sbjct: 520 CNYPDHVALVLKRLNGSFRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALY 579

Query: 609 LLTCPLSDANDGEKQLRAAQMVFLNTPHPLETLSDRSAYGSGGTIQRDHDMNSYLKSVRG 668
           +L       N  E     A   FLN PHPLETLS R+AYGS G++ RDHD  +Y+K+V G
Sbjct: 580 IL------ENSNEGYSPTALRAFLNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNG 633

Query: 669 VIRQELNRIRKARRQHRRKVWWALVAPGK 698
           V+RQ    I +  R  RR VW  L + G+
Sbjct: 640 VLRQHTKLIVRKARIQRRSVWPVLTSAGR 633

BLAST of Lsi02G024620 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 9.4e-06
Identity = 35/91 (38.46%), Postives = 48/91 (52.75%), Query Frame = 0

Query: 461 VLEHLKSHGDRATFRFTGHSLGGSLALLVNLMLLIRNEVPV-SSLLPVITFGAPSIMCGG 520
           +L+ L S  + A F  TGHSLGG+LA+L   +L++  E  +   LL V TFG P I   G
Sbjct: 320 ILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRI---G 379

Query: 521 DR-----LLRKLGLPRNHLQAVTLHRDIVPR 546
           +R     +  +L  P +    V    DIVPR
Sbjct: 380 NREVGLFMKAQLNQPVDRYFRVVYCNDIVPR 407

BLAST of Lsi02G024620 vs. TAIR 10
Match: AT5G18640.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.8e-04
Identity = 36/132 (27.27%), Postives = 59/132 (44.70%), Query Frame = 0

Query: 420 SLASWQANLLFEPIDF---EGLGVLVHRGIYEAAKGMYEQMLPDVLEHLK----SHGDRA 479
           S+ +W ++L ++ +D    +    +VH G Y A       + P VL+ +K    S+G   
Sbjct: 111 SIQNWVSDLFWKQLDLNYPDMPDAMVHHGFYSAYHN--TTVRPAVLDAVKRAKESYGANL 170

Query: 480 TFRFTGHSLGGSLALLVNLMLLIRNEVPVSSLLPVITFGAPSIMCGGDRLLRKLGLPRNH 539
               TGHS+GG++A    L L++         + V+TFG P +  G         L   +
Sbjct: 171 NIMVTGHSMGGAMASFCALDLVVNEG---EENVQVMTFGQPRV--GNAAFASYFNLLVPN 230

Query: 540 LQAVTLHRDIVP 545
              +   RDIVP
Sbjct: 231 TFRIIHDRDIVP 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4HXL01.7e-22258.60Phospholipase A1 PLIP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP2 P... [more]
Q940L45.0e-16953.11Phospholipase A1 PLIP3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP3 P... [more]
Q7Y2204.3e-12845.87Phospholipase A1 PLIP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP1 P... [more]
F4JFU81.3e-0438.46Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BRN40.0e+0092.04uncharacterized protein LOC103492768 OS=Cucumis melo OX=3656 GN=LOC103492768 PE=... [more]
A0A5A7TTC60.0e+0091.77Lipase, class 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold427G0050... [more]
A0A0A0K9H80.0e+0090.95Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407550 PE... [more]
A0A5D3D3D90.0e+0087.52Lipase, class 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G0015... [more]
A0A6J1GN820.0e+0088.69uncharacterized protein LOC111455991 OS=Cucurbita moschata OX=3662 GN=LOC1114559... [more]
Match NameE-valueIdentityDescription
XP_038899170.10.0e+0093.26phospholipase A1 PLIP2, chloroplastic [Benincasa hispida][more]
XP_011659388.10.0e+0092.17phospholipase A1 PLIP2, chloroplastic [Cucumis sativus] >KAE8646362.1 hypothetic... [more]
XP_008451496.10.0e+0092.04PREDICTED: uncharacterized protein LOC103492768 [Cucumis melo][more]
KAA0046682.10.0e+0091.77Lipase, class 3 [Cucumis melo var. makuwa][more]
TYK18218.10.0e+0087.52Lipase, class 3 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G02660.11.2e-22358.60alpha/beta-Hydrolases superfamily protein [more]
AT3G62590.13.5e-17053.11alpha/beta-Hydrolases superfamily protein [more]
AT3G61680.13.0e-12945.87alpha/beta-Hydrolases superfamily protein [more]
AT3G14360.19.4e-0638.46alpha/beta-Hydrolases superfamily protein [more]
AT5G18640.16.8e-0427.27alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 348..368
NoneNo IPR availableCOILSCoilCoilcoord: 760..765
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..78
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..90
NoneNo IPR availablePANTHERPTHR46483:SF4PHOSPHOLIPASE A1 PLIP2, CHLOROPLASTICcoord: 51..761
NoneNo IPR availableCDDcd00519Lipase_3coord: 394..545
e-value: 7.16992E-25
score: 101.784
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 348..577
e-value: 1.1E-28
score: 102.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 396..579
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 413..550
e-value: 1.1E-25
score: 90.2
IPR043367Phospholipase A1 PLIP1/2/3, chloroplasticPANTHERPTHR46483PHOSPHOLIPASE A1 PLIP2, CHLOROPLASTICcoord: 51..761

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G024620.1Lsi02G024620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0008970 phospholipase A1 activity