Lsi02G024340 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G024340
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPectinesterase
Locationchr02: 30889968 .. 30896617 (-)
RNA-Seq ExpressionLsi02G024340
SyntenyLsi02G024340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACGGTCTCAAAAACAGAGCTCAAATCCCTTCGAATCCCACCTCCATTTCCACCTTCTTCTTCATTTTCTTCCTCTTTTCCTTCAAACCCATTTCTTCAAATCCCCAAATCCACCACTCCGCTCGATCCCTTTGCAATGGCACTCTCTTTCCAAACCTCTGTTTCTCAAAACTCTCCTCTTTTCCCCAATTGGCTTCCCTCTCGCCGGAAAAGCTCGCTGGCAGTGCCCTCAACTTCACTACCCGCGAGGTCCTCGGTGCCTACGCCAATTGCACCAAGCTCAAGACTGTCCTTTATGGTGGCCTGAATCCCACCGACCGCCGTGCTCTCGACGACTGTCTTGAGCTTCTCGACGGTAGCATTGCCGAGCTCAAAGCTTCGATTTTCGATCTAGCGCTGCCGCAGTCCGCTGCCCGTCACTCCCATGATCTGTTGACATTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTGTTGATGGGTTTTATAATAGTTCCGGGACTGTGAGGAGCAGGGTGGAGCTGTATTTGGGGAAAATTGGACAGCATTTAAGTATTGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTGCGGCGGCGGGGGAGGTGTTGCCGGAGTATGGGACGGTGAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCTGTGAATGAGACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTTACCACTGTCGGTGAGGCCATCGCCGCCGCTCCGAATTCGAGTACCACCAGGTTTTGTTGAACTGAACTCCTGAATTTTTTTTATTAAAAAATTATTAAGTGAAGTGAGAAACTGAACTTTTGATTAAAAAATTATTTGTTATTAAACAGAAATGATTAAAGAAAAAGTAAAATGACTTTTTGATTAAATTTTAAAAATTAAAAGAGAAATAATTGAAAGATTAAGGATTTTTTTTTTAGTACCATAGAGGTGGAAACATTTAAATCACAACCACGTAATTCTTAATTACTAATACATATTCTGGTTTTTTTTTTAAGTTATTTTATTTTAGCTTTTAATTATTTTTAAAAGATTGTTATATTGTAGATAGTGTTTGTCCAGCTACTACCTAGCTCAACTGCTAGTTTAATATAGTTTAAACTATCTATTTAAATAGTAGATGATAGATTTTGTTTTTCAAAAAGTTTCCTACTAAAATAAAACCTTAAATTTAGCAACGTAAATAAAAAATTAAATTAAATTTATTTACCAAAGTATAATCGATAAAATATTTGAAAGTTTGAAGAAGAAAATATAGTATATTTTTCCATTTATATCATATCCAACTTTTATACGCTGATCTAAAAAAAGGAAAAAAAATGTTTATTTTATTTAAATTAATTAATTAATTAATTTGTAGTACTGAAAATACAATTCTAAAAAGTGAGGAAATAAAATTAAAACATTATTAATGAACAAGATACTCTGTTATAAAACACGACAGGATAACTGTATAGTATACTTGCCAAATTTAATCATCCTTAACAAGAAAAAAAAACAAATTTATTTAATCATCACATCGTACAATAAAAGTAATACTTCTAAAAGATCTCTTTATTGATACGAAACCTCAATGCATTATTAAATTATTTGTCATATCACATCAATCCACTTAAAAGTAAGATTTTAAATTACTTTTAATTCTCAAACTTTCGTGAAGGTAACAATTTAGTCTCTAAATTTTAATTTGTAACGATTTAGTTAATATATTTCTAATTTTATAATAATTTAGTTTCTACATTTTAGTATGCAATAATTTAATATATATATAATTATTTAATATTTAGCGTAAACATTTTTGTTAAAATTTAATAAAATTTCATATTTGAATAGATTTTTAATAAATTAATTAGAGATTCAATATGTTTATAATCTGCAAAGTTTAAATTGTTAAATAATAGACATTGACATTTAATTATGTTAGAATTTTTATGTTGTTAAATTATTATATCTTAAAGTAAACCGATTAAATTGTTATTTTTTATGAAAATTGGACAAAAAAAAACATGATTTTTAACTTAAAAACAATCTAAAAGACAAAAATTAAATAAAGTTACCAAGATTAAATTTATAATCATAAGATTTTTAAAATGGTAATTAAGAGAAAATGTGAATTTCTTATGGGACAGGTTTGTAATATACATAAAAGCAGGGGCGTATTTTGAGAACGTGGAAATAGAAAGGGCAAAGAGAAACTTGATGTTTGTCGGCGACGGGATCGGAAAGACGCTGATAAAAGCCGACCGGAACGTCGTGGACGGCTGGACTACTTTCCGATCAGCCACCGTCGGTAAGTTCTCTTTTTAACTCTTTATTTTTTCTTAAAAAGAAATAAAATTATTAATTGCGATTACTAAAATGATGAAACCACCTGACAAAGATCAGATCTTGCGTCTGAAATGATTTCTTTAAGTTCGTAGTTTAATTATTATTTAATAATACAATCTTGGAAAGATTCTATCAGCGGAAGAAAATTTTGGATCTGTTTCAATATTTTATATTTAGAAAACATTTCTTAGAAAAACTTTCTAATAACAGATTTATTGAAAGATTACCTTTTTAATTTCTGAATTTTAAAGAATCCGTCCCTTTGATCTAAGTTTTAAAATGTATTTTTTTAGTTTTTAATTTTTTAAAAATAGATGGGTTTGAATTCCGAGTTTTCAAAATAAGTTTAAGGTTCCTCAAATAATTTATTATTATTTAAAATAATGATATGACATTTTAAATTAACTTGAAGACTAAAAATGTATATTTTTAAGACTCAAGGACCAAATACACATTCTTCAAAACTTATGGACTAAAAAGATAATTTTTTTTAAAATTTATGTTATTTCTAAAAAAAACTTTTTTATTGTAATTTTTACGAAGCTCAAGGAAATAATTGAAGTTGTAGTCAATTTTTAATAACAAAAACAATTTTTTTAAAACTATGATCTAATTTGATTTAATATATTTTTTAAAATTTTGTTCTTAATTTCTGACTATTTCTTAACCGTAGTTTTCATCTTTCGACTTTTATTTTTCATTAATAAGTTTCTCACAATCTCTAATTTTATAATTATTTTCTCACCATTATAAATTTTTTCAAAACATATCTCAATACAAAATTTAAAAGTTTTTTCTTTTTTTTTTTTTTTTTTTGACATACGAAATTTGGAAAGTTTAGCGATTTATTTTAATTCTTGTAATTATTTATTCAGTAGACTAAAGTTTGTAAATGTTAAATGTACCCTCGAAAGAAAGTAAAAATTTAAATTTCTTTTATAAAAGAAGTCAAAAGGAAAGCTTTTAGAAAAGAATCGCAGTAAGGTGTAGGGGTAATGGGAAGGCGAGTAGAGTGGGGTCCAGAGGGAGCAAAAAGGCCTAAAAAATGATCAAACCGCGTGTCGGTTGGATAAGATTGCTACTTACTTGGGACAAAAGGCCCGTTGCCTTTTTCCATTTTTTTTCCTAAGTTTTCTACTTACAAAATTTTGGGAAATATATCTTTCCAATTAAGGATGGCAATGGGTGTGGGTTGGGATGCAATTTTCGTCTCTGTCTCTACCTTCACCTCATTCTCTATTTTAGCACCAAAATTGAAAAATATTGGTTCGGAGATTTAGGTCCCTGTACAAAAATCCTCACTTTTTATTTTATTATTTAAAATTAATTAAATCTTACTATTTATATAGATATTTTAGGTAAGAAGTTATTATTATTAGGGTTGTTTTCAAATATAAAAAAATGTGTCAAACTATTTACACATATAGAAAAAAATTTACAGTATATCAGTGATAGATCACGATAGAATTCTATCGCTTTCTATCGCTCAAGCGATAAAATTTATTTACTTGAGCGATAGGAAGCGATATAATTCTATCGCGGTCTATCATTGATAGACAGTGAAATTTTTCTATATTTGTAAATAGTTTAATATTTTTTCTGTTTATAGTAATTTCTGTCGTTGGTTATTATTATTATTTTAAATGAATAATTAAAAATAATATTTTAGTTCTTTTTATTATAAATTTAATAAAAATAAATAAATTTATTATATTAAAAAAATGAAAATTAGGGGAAAAAATCAAAGTTAAACTAATATAGTTCCTTTGAGTAGCTGAAAAGCTACTGTTTTAGTAGTCTCTCTATATAAATATACATATATATATATATATATATAATTAAAAAATTCGAGGCAGGGGCGAGGTTCGGGGATCTCATCTCCGTCCTCGCCTCGTCCCCATCAGGCACCCAAATTAAATCTCTGATTGATCTCCGTACTCGATCAAACTGGAGATTCTCCACCCCGATTGAGTCGGGGACATATGGAAAATTTTGCCATCCCTATTTCCAACCCTAGGTTTTTTAAACAAATTTTCATTTAGTCTTTGTTTTTTTTGCAAATTACAAAATCACTCTTTTAAATGTTAATAGTTATAATTATACCTCAAAATTTTGATTATAAAAATTGAACCCTTAAACTTATATAACTGAAAAGATTTGACCTTCAAACTTACATAATTGTAGAAATTGTATAAGTTTGATGGTTCAAATTTTACTATTTAGGGGTCCAATTTTTACCACTATTGTAAATTTGAGGGTTCAATTTTTAGTAGCTAACATATATTATATAGAAGTTATATTTGTTGTATCTTTTCATTTGGTTCTTAAATTTCAAAATATCTTACCTTTCAAATTTTGAGTTTAATTTCAATTTGATTTCTATGTTCTAAAATGTTATATTGTTATCCTTACATTTTGAGTTTAGTTTTTGTTTGGTCATTTTATTTCTTTTTATTTTGTATTTTAACCCTCAAGTTTTCACCAATGCTTACTTTTGGTATTTGTCATTAAATTTTAGTTAATTGATTTAAAATAATTATGATGTCAATTTTTTTTTTATAAATTTTAATAATGATATAAAATTAGTGAAAATCTTTTAAATTATAATTAGTCTAATTTTTTTAAATTTATTAATAGACATCCACACAAAAAATTGGAAGTGAACATTTAATTTAGCATAGAAATTAAGAGTAAAAATATAAATCTTGAAACATTGGGACTAAATTGAAACAAAATTCAAAGATAAAAATATAAAATTTTGAAATCTATGGACTAAATAGAAGGTACTCAAAACTCAAATGTAAATGAAACCTAACAACCCAACATAAACTAGACTAAACTATCAATTTTCTATCTAATAAAATCTTAAATTTTAAAAATATTAAATAGATCTTTGAACTTTTGAATGCATTTTTTTTAAATAACAAATCTATAAGACATAAAGTTAAATTTTATATTTACTAAGATATTAAACTTACAATATTGTGTCCAATAGTTTCGTAAATGTTATCAAATAAATCAAAATCTATTAGACATAGTAAAAAATTCTTAAATTACCAATTGACCCAAAAATTTAAACCGATGGATGAAAATAAATTTAATATTATATTCTCTAACACTCCCCTCACTTGTGATGAACTTGAAATATGTAAAACCCAACAAGTGTGAAATCAATATCAACAGAGGAGAAAATAATAGGGTTTAAACACAAGACCTCTTGTCCTGATACTATCTTAATTCACCAATTAAAATTTTTTGGTGACTTATTAGCTACTTTCTAAAATTCAAGGATGTTAAATCTTCTAGCAATAGGTCGAACATAAACATAACTCAACAATAGTTGACACATACTCTTTTTCTTAAGGCCGACCAAAAGTTTAAATCTTCATACTCTACATCACATTTGTTGTACTTAAAAAAACAGTACTTTCTCTATGTAATTAATCGTTATTTAAATATACTCATACTTGTTTGATATTATAGTTTAAAATTAACAAAAGTGGATTCAAATTTTATTCATTTATTAGCAATAACACAAGTTGGTTTATTATGCAGCCGTGGTTGGCACCGGGTTCATAGCAAAAGGCATAACATTCGAAAACTACGCGGGTCCAAGCAAGCACCAAGCCGTAGCCTTACGGAGCAACTCTGACTTCTCTGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTATGTTCACTCTCTCCGTCAATTCTACCGTGACTGCGATATCTACGGTACCATCGACTTCATTTTCGGCAATGCTGCCGTCGTCTTCCAAAACTGCAACCTCTACGCTCGAAAACCCAACCCAAACCAACGCAACATATTCACTGCTCAAGGTCGCGAGGACCCTAACCAAAACACTGGAATCTCAATCCTCAATTGTAAGGTTGAAGCCGCCTCCGACCTAATCCCGGTTCTTTCCTCGTTCAGAACTTACTTGGGCCGACCATGGAAGTTGTACTCGAGGACGGTTTTTCTTCGGTCGTTTATCGGTCAGTTGGCTGACCCAGCTGGGTGGCTTGAGTGGAACGGTACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTGAACCGGGGGCCTGGTTCAAATACGACTGGACGAGTTACTTGGCCAGGTTATCGAGTCATTACTAACGCAACAGAAGCGAATCAGTTTACAGTCGAAGCTTTTATACAAGGTAGCTCGTGGTTGAACTCGACCGGGATTCCTTTCTTCTCCGGTTTGGCTTGA

mRNA sequence

ATGTACGGTCTCAAAAACAGAGCTCAAATCCCTTCGAATCCCACCTCCATTTCCACCTTCTTCTTCATTTTCTTCCTCTTTTCCTTCAAACCCATTTCTTCAAATCCCCAAATCCACCACTCCGCTCGATCCCTTTGCAATGGCACTCTCTTTCCAAACCTCTGTTTCTCAAAACTCTCCTCTTTTCCCCAATTGGCTTCCCTCTCGCCGGAAAAGCTCGCTGGCAGTGCCCTCAACTTCACTACCCGCGAGGTCCTCGGTGCCTACGCCAATTGCACCAAGCTCAAGACTGTCCTTTATGGTGGCCTGAATCCCACCGACCGCCGTGCTCTCGACGACTGTCTTGAGCTTCTCGACGGTAGCATTGCCGAGCTCAAAGCTTCGATTTTCGATCTAGCGCTGCCGCAGTCCGCTGCCCGTCACTCCCATGATCTGTTGACATTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTGTTGATGGGTTTTATAATAGTTCCGGGACTGTGAGGAGCAGGGTGGAGCTGTATTTGGGGAAAATTGGACAGCATTTAAGTATTGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTGCGGCGGCGGGGGAGGTGTTGCCGGAGTATGGGACGGTGAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCTGTGAATGAGACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTTACCACTGTCGGTGAGGCCATCGCCGCCGCTCCGAATTCGAGTACCACCAGGTTTGTAATATACATAAAAGCAGGGGCGTATTTTGAGAACGTGGAAATAGAAAGGGCAAAGAGAAACTTGATGTTTGTCGGCGACGGGATCGGAAAGACGCTGATAAAAGCCGACCGGAACGTCGTGGACGGCTGGACTACTTTCCGATCAGCCACCGTCGCCGTGGTTGGCACCGGGTTCATAGCAAAAGGCATAACATTCGAAAACTACGCGGGTCCAAGCAAGCACCAAGCCGTAGCCTTACGGAGCAACTCTGACTTCTCTGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTATGTTCACTCTCTCCGTCAATTCTACCGTGACTGCGATATCTACGGTACCATCGACTTCATTTTCGGCAATGCTGCCGTCGTCTTCCAAAACTGCAACCTCTACGCTCGAAAACCCAACCCAAACCAACGCAACATATTCACTGCTCAAGGTCGCGAGGACCCTAACCAAAACACTGGAATCTCAATCCTCAATTGTAAGGTTGAAGCCGCCTCCGACCTAATCCCGGTTCTTTCCTCGTTCAGAACTTACTTGGGCCGACCATGGAAGTTGTACTCGAGGACGGTTTTTCTTCGGTCGTTTATCGGTCAGTTGGCTGACCCAGCTGGGTGGCTTGAGTGGAACGGTACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTGAACCGGGGGCCTGGTTCAAATACGACTGGACGAGTTACTTGGCCAGGTTATCGAGTCATTACTAACGCAACAGAAGCGAATCAGTTTACAGTCGAAGCTTTTATACAAGGTAGCTCGTGGTTGAACTCGACCGGGATTCCTTTCTTCTCCGGTTTGGCTTGA

Coding sequence (CDS)

ATGTACGGTCTCAAAAACAGAGCTCAAATCCCTTCGAATCCCACCTCCATTTCCACCTTCTTCTTCATTTTCTTCCTCTTTTCCTTCAAACCCATTTCTTCAAATCCCCAAATCCACCACTCCGCTCGATCCCTTTGCAATGGCACTCTCTTTCCAAACCTCTGTTTCTCAAAACTCTCCTCTTTTCCCCAATTGGCTTCCCTCTCGCCGGAAAAGCTCGCTGGCAGTGCCCTCAACTTCACTACCCGCGAGGTCCTCGGTGCCTACGCCAATTGCACCAAGCTCAAGACTGTCCTTTATGGTGGCCTGAATCCCACCGACCGCCGTGCTCTCGACGACTGTCTTGAGCTTCTCGACGGTAGCATTGCCGAGCTCAAAGCTTCGATTTTCGATCTAGCGCTGCCGCAGTCCGCTGCCCGTCACTCCCATGATCTGTTGACATTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTGTTGATGGGTTTTATAATAGTTCCGGGACTGTGAGGAGCAGGGTGGAGCTGTATTTGGGGAAAATTGGACAGCATTTAAGTATTGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTGCGGCGGCGGGGGAGGTGTTGCCGGAGTATGGGACGGTGAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCTGTGAATGAGACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTTACCACTGTCGGTGAGGCCATCGCCGCCGCTCCGAATTCGAGTACCACCAGGTTTGTAATATACATAAAAGCAGGGGCGTATTTTGAGAACGTGGAAATAGAAAGGGCAAAGAGAAACTTGATGTTTGTCGGCGACGGGATCGGAAAGACGCTGATAAAAGCCGACCGGAACGTCGTGGACGGCTGGACTACTTTCCGATCAGCCACCGTCGCCGTGGTTGGCACCGGGTTCATAGCAAAAGGCATAACATTCGAAAACTACGCGGGTCCAAGCAAGCACCAAGCCGTAGCCTTACGGAGCAACTCTGACTTCTCTGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTATGTTCACTCTCTCCGTCAATTCTACCGTGACTGCGATATCTACGGTACCATCGACTTCATTTTCGGCAATGCTGCCGTCGTCTTCCAAAACTGCAACCTCTACGCTCGAAAACCCAACCCAAACCAACGCAACATATTCACTGCTCAAGGTCGCGAGGACCCTAACCAAAACACTGGAATCTCAATCCTCAATTGTAAGGTTGAAGCCGCCTCCGACCTAATCCCGGTTCTTTCCTCGTTCAGAACTTACTTGGGCCGACCATGGAAGTTGTACTCGAGGACGGTTTTTCTTCGGTCGTTTATCGGTCAGTTGGCTGACCCAGCTGGGTGGCTTGAGTGGAACGGTACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTGAACCGGGGGCCTGGTTCAAATACGACTGGACGAGTTACTTGGCCAGGTTATCGAGTCATTACTAACGCAACAGAAGCGAATCAGTTTACAGTCGAAGCTTTTATACAAGGTAGCTCGTGGTTGAACTCGACCGGGATTCCTTTCTTCTCCGGTTTGGCTTGA

Protein sequence

MYGLKNRAQIPSNPTSISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLELLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPFFSGLA
Homology
BLAST of Lsi02G024340 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 3.1e-152
Identity = 285/520 (54.81%), Postives = 361/520 (69.42%), Query Frame = 0

Query: 40  HSARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVL 99
           H   SL    LF +  FS   +    +S    +L  +ALN T   V  + +N + L   L
Sbjct: 7   HIILSLTFLLLFLSTVFSSHVTQRFTSSDDITELVVTALNQTISNVNLSSSNFSDLLQRL 66

Query: 100 YGGLNPTDRRALDDCLELLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTC 159
              L+  D  A DDCLELLD ++ +L  +I  L   +S +   H++  L+SAAMTN RTC
Sbjct: 67  GSNLSHRDLCAFDDCLELLDDTVFDLTTAISKL---RSHSPELHNVKMLLSAAMTNTRTC 126

Query: 160 VDGFYNSSGT------------VRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAAAAGEV 219
           +DGF +S               V   ++  L  I  H+S  LAML+ IPG       G+V
Sbjct: 127 LDGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPG----HIPGKV 186

Query: 220 LPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTT 279
             +      GFP WVS  DR LLQ  V+ETK NLVVA++G+GN+TT+GEAI+AAPNSS T
Sbjct: 187 KEDV-----GFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSET 246

Query: 280 RFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTG 339
           RFVIYIK G YFEN+EI R K  +MF+GDGIG+T+IKA+R+  DGWT F SATV V G+G
Sbjct: 247 RFVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSG 306

Query: 340 FIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGT 399
           FIAK ++F NYAGP KHQAVALRS+SD SA+Y+CSF  YQDT+YVHS +QFYR+CDIYGT
Sbjct: 307 FIAKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGT 366

Query: 400 IDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPV 459
           +DFIFG+A+VVFQNC+LYAR+PNPNQ+ I+TAQGRE+  + TGISI++ ++ AA DLIPV
Sbjct: 367 VDFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPV 426

Query: 460 LSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNT 519
            ++F+ YLGRPW+LYSRTV ++SFI  L DPAGWL+W   FAL+TLYYGEY+N GPGSN 
Sbjct: 427 QANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNM 486

Query: 520 TGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPF 548
           T RV WPG++ I    EA+QF+V  FI G+ WLNST IPF
Sbjct: 487 TNRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

BLAST of Lsi02G024340 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 6.8e-144
Identity = 275/542 (50.74%), Postives = 349/542 (64.39%), Query Frame = 0

Query: 17  ISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGS 76
           +S  FF+ FL +     S     H  + L      P L  +                   
Sbjct: 19  LSLIFFLIFLSTVVSSQSPSYTTHKTQRLTETKTIPELIIAD------------------ 78

Query: 77  ALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLELLDGSIAELKASIFDLALPQ 136
            LN T  +V  A +N + L+T L+  L   +R A +DCL LLD +I++L+ ++ DL   +
Sbjct: 79  -LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---R 138

Query: 137 SAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSID 196
           S++   +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  
Sbjct: 139 SSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNS 198

Query: 197 LAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGS 256
           L ML+ I     +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+
Sbjct: 199 LHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGT 258

Query: 257 GNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRN 316
           GNFTT+ +A+ AAPN S TRF+IYIK G YFENVE+ + K  +MF+GDGIGKT+IKA+R+
Sbjct: 259 GNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRS 318

Query: 317 VVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQD 376
            +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQD
Sbjct: 319 RIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQD 378

Query: 377 TLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQN 436
           TLYVHS +QFYR+CDIYGTIDFIFGNAAVVFQN +LYARKPNP  +  FTAQ R   +Q 
Sbjct: 379 TLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQP 438

Query: 437 TGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTF 496
           TGISILNC++ AA DLIPV  +F+ YLGRPW+ YSRTV ++SFI  L  PAGWLE    F
Sbjct: 439 TGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDF 498

Query: 497 ALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPFF 551
           AL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA QFTV  FI GS+WLNSTGIPF 
Sbjct: 499 ALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFS 531

BLAST of Lsi02G024340 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.5e-143
Identity = 247/319 (77.43%), Postives = 273/319 (85.58%), Query Frame = 0

Query: 233 AVNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLM 292
           AV +   ++VVAKDGSGNFTTVG A+AAA +SST RFVIYIK GAYFE V++++ K NLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 293 FVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSN 352
           F+GDGIGKT IK +R+VVDGWTTFRS+TVAVVGTGFIA+GI+FENYAGPSKHQAVALRS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 353 SDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPN 412
           +DFSAFYQCSF+GYQDTLYVHSLRQFY +CD+YGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 413 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFI 472
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DLIPVLSSF+TYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 473 GQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEA 532
             L DPAGWLEW+G FAL TLYY EY N GPGSNTT RVTWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 533 FIQGSSWLNSTGIPFFSGL 552
           FIQGS WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

BLAST of Lsi02G024340 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.3e-139
Identity = 269/530 (50.75%), Postives = 351/530 (66.23%), Query Frame = 0

Query: 33  SSNPQIHHSARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTTREVLGAYA 92
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN TT  V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 93  NCTKLKTVLYGGLNPTDRRALDDCLELLDGSIAELKASIFDL---ALPQSAARHSHDLLT 152
              KL  +    L   ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 153 LVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--IGQHLSIDLAMLKKIPG----VNRA 212
           L+SAAMTN  TC+DGF +       R  L  G+  + +  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 213 AAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAA 272
           +   +++ E  TV  G+P W+S  DRRLLQ+  +    N+VVA DGSGNF TV  ++AAA
Sbjct: 240 SNNRKLIEETSTV-DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 299

Query: 273 PNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATV 332
           P   T R++I IKAG Y ENVE+ +  +N+MF+GDG  +T+I   RNVVDG TTF+SATV
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 359

Query: 333 AVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRD 392
           AVVG GF+A+ ITF+N AGPSKHQAVALR  +D SAFY C  + YQDTLYVHS RQF+ +
Sbjct: 360 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 419

Query: 393 CDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAA 452
           C I GT+DFIFGNAA V QNC+++ARKPN  Q+N+ TAQGR DPNQNTGI I   ++ A 
Sbjct: 420 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGAT 479

Query: 453 SDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNR 512
           SDL PV  SF TYLGRPWK YSRTV ++S I  +  PAGW EW+G FAL+TL+YGE+ N 
Sbjct: 480 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNA 539

Query: 513 GPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPFFSGL 552
           G G+ T+GRV W G+RVIT+ATEA  FT  +FI GSSWL STG PF  GL
Sbjct: 540 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lsi02G024340 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 8.6e-139
Identity = 270/530 (50.94%), Postives = 348/530 (65.66%), Query Frame = 0

Query: 33  SSNPQIHHSARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTTREVLGAYA 92
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN TT  V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 93  NCTKLKTVLYGGLNPTDRRALDDCLELLDGSIAELKASIFDL---ALPQSAARHSHDLLT 152
              KL  +    L   ++ AL DCLE +D ++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 153 LVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--IGQHLSIDLAMLKKIPG----VNRA 212
           L+SAAMTN  TC+DGF +       R  L  G+  + +  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 213 AAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAA 272
           +   ++  E  TV  G+P W+S  DRRLLQ+  +    N VVA DGSGNF TV  A+AAA
Sbjct: 240 SNNRKLTEETSTV-DGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAA 299

Query: 273 PNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATV 332
           P   T R++I IKAG Y ENVE+ +  +N+MF+GDG  +T+I   RNVVDG TTF+SAT 
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATA 359

Query: 333 AVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRD 392
           AVVG GF+A+ ITF+N AGPSKHQAVALR  +D SAFY C  + YQDTLYVHS RQF+ +
Sbjct: 360 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 419

Query: 393 CDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAA 452
           C I GT+DFIFGNAA V QNC+++ARKPN  Q+N+ TAQGR DPNQNTGI I   ++ A 
Sbjct: 420 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGAT 479

Query: 453 SDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNR 512
           SDL PV  SF TYLGRPWK YSRTV ++S I  L  PAGW EW+G FAL+TL+YGE+ N 
Sbjct: 480 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNS 539

Query: 513 GPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPFFSGL 552
           G G+ T+GRV W G+RVIT+ATEA  FT  +FI GSSWL STG PF  GL
Sbjct: 540 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lsi02G024340 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 1035.4 bits (2676), Expect = 8.5e-299
Identity = 518/554 (93.50%), Postives = 525/554 (94.77%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTS---ISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFS 60
           MYGL+NRAQI SNPTS   ISTFFFIFFLFS KPISSNPQIH +ARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVL AYANCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL   QSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA    EVLPEYG VKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EA+AAAPNSSTTRFVIYIKAGAYFENVEI RAK NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK DRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYR+CDIYGTIDFIFGNAAVVFQNCNLYARKPN NQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLAD 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL +
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGS 540
           P GWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEA+QFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGL 552
           SWLNSTGIPFFSGL
Sbjct: 541 SWLNSTGIPFFSGL 554

BLAST of Lsi02G024340 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.1e-298
Identity = 517/554 (93.32%), Postives = 525/554 (94.77%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTS---ISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFS 60
           MYGL+NRAQI SNPTS   ISTFFFIFFLFS KPISSNPQIH +ARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVL AYANCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL   QSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA    EVLPEYG VKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EA+AAAPNSSTTRFVIYIKAGAYFENVEI RAK NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK DRNVVDGWTTFRSATVAV+GTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYR+CDIYGTIDFIFGNAAVVFQNCNLYARKPN NQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLAD 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL +
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGS 540
           P GWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEA+QFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGL 552
           SWLNSTGIPFFSGL
Sbjct: 541 SWLNSTGIPFFSGL 554

BLAST of Lsi02G024340 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 499/556 (89.75%), Postives = 520/556 (93.53%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPT----SISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCF 60
           MYGL++RAQ  SNPT    +I T F + FLFSFKPISSNPQ H +ARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV GAYANC+KLKT+ YGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLDGSIAEL+ SI+DL+  QSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RAA A EVLPEYG V+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEA+AAAPNSSTTRFVIYIKAGAYFENVEIER K NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVA VG GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPN NQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLA 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRT+FL+SFIGQL 
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLV 480

Query: 481 DPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQG 540
           +P GWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEA+QFTVEAFIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SSWLNSTGIPFFSGLA 553
           S WLNST IPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Lsi02G024340 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 1.8e-288
Identity = 493/556 (88.67%), Postives = 517/556 (92.99%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPT----SISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCF 60
           MYGL++RAQ  SNPT    +I TFF + FL SFKPISSNPQ H +ARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV GAYANC+KLKT+ YGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLDGSIAEL+ SI+DL+  QSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RAA A EVLPEYG V+GGFP WVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGD 300
           TKFNLVVAKDGSGNFTTVG A+AAAPNSSTTRFVIYIKAGAYFENVEIER K NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATV  VG GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAV+FQNCNLYARKPN NQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLA 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRTVFL+SFIGQL 
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 DPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQG 540
           +P GWLEW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEA+QFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SSWLNSTGIPFFSGLA 553
           S WLNST IPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Lsi02G024340 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.2e-276
Identity = 479/522 (91.76%), Postives = 490/522 (93.87%), Query Frame = 0

Query: 31  PISSNPQIHHSARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYA 90
           P+      H +ARSLCNGTLFP+LCFSKLSSFPQLASLSPEKLAGSALNFT+REVL AY 
Sbjct: 90  PLPLFQTFHQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYT 149

Query: 91  NCTKLKTVLYGGLNPTDRRALDDCLELLDGSIAELKASIFDLALPQSAARHSHDLLTLVS 150
           NCT LKT LY GLNPTDR ALDDCLELLDGSIAELKASIFDLA  QS A HSHDLLTLVS
Sbjct: 150 NCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVS 209

Query: 151 AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAAAAG-EVLP 210
           AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRA     E+LP
Sbjct: 210 AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLP 269

Query: 211 EYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRF 270
           EYG VKGGFPKWVSVKDRRLLQAAVNETKFN+VVAKDGSGNFTTV EA+AAAPN+STTRF
Sbjct: 270 EYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRF 329

Query: 271 VIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFI 330
           VIYIKAGAYFENVEI RAK NLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFI
Sbjct: 330 VIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFI 389

Query: 331 AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTID 390
           AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCD+YGTID
Sbjct: 390 AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTID 449

Query: 391 FIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 450
           FIFGNAAVVFQNCNLYARKPN NQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS
Sbjct: 450 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 509

Query: 451 SFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTG 510
           SFRTYLGRPWKLYSRTVFLRSFIGQL +P GWLEWNGTFALDTLYYGEYLNRGPGSNTT 
Sbjct: 510 SFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTM 569

Query: 511 RVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPFFSGL 552
           RVTWPGYRVITNATEA+QFTVE FIQGSSWLNST IPFFSGL
Sbjct: 570 RVTWPGYRVITNATEASQFTVERFIQGSSWLNSTEIPFFSGL 611

BLAST of Lsi02G024340 vs. NCBI nr
Match: XP_038896904.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.5e-308
Identity = 527/555 (94.95%), Postives = 538/555 (96.94%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTS---ISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFS 60
           MYGLKNRAQIPSNPTS   ISTFFFIFF FSFKPISSNPQIH +ARSLCNGTLFP+LCFS
Sbjct: 1   MYGLKNRAQIPSNPTSISTISTFFFIFFFFSFKPISSNPQIHQTARSLCNGTLFPHLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLEL 120
           KLSSFPQLASLSPEKLAGSA+NFTTREVLGAYANCTKLKT+LYGGLNPTDRRALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLEL 120

Query: 121 LDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAEL+ASI DLA PQSAARHSHDLLTL+SAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA AAGEVLPEYG VKGGFP WVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDG 300
           KFNLVVAKDGSGNFTTVGEA+AAAPNSSTTRFVIYIKAGAYFENVEIER K NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDG 300

Query: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIKADRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIF 420
           FYQCSF+GYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPN NQRNIF
Sbjct: 361 FYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLAD 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQL D
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVD 480

Query: 481 PAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGS 540
           PAGWLEWNGTFALDTLYYGEY+NRGPGSNTTGRVTWPGYRVITNA+EA+QFTVE FIQGS
Sbjct: 481 PAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGS 540

Query: 541 SWLNSTGIPFFSGLA 553
           SWLNSTGIPFFSGLA
Sbjct: 541 SWLNSTGIPFFSGLA 555

BLAST of Lsi02G024340 vs. NCBI nr
Match: XP_008451545.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.8e-298
Identity = 518/554 (93.50%), Postives = 525/554 (94.77%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTS---ISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFS 60
           MYGL+NRAQI SNPTS   ISTFFFIFFLFS KPISSNPQIH +ARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVL AYANCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL   QSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA    EVLPEYG VKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EA+AAAPNSSTTRFVIYIKAGAYFENVEI RAK NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK DRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYR+CDIYGTIDFIFGNAAVVFQNCNLYARKPN NQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLAD 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL +
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGS 540
           P GWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEA+QFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGL 552
           SWLNSTGIPFFSGL
Sbjct: 541 SWLNSTGIPFFSGL 554

BLAST of Lsi02G024340 vs. NCBI nr
Match: KAA0046658.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.3e-298
Identity = 517/554 (93.32%), Postives = 525/554 (94.77%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTS---ISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFS 60
           MYGL+NRAQI SNPTS   ISTFFFIFFLFS KPISSNPQIH +ARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVL AYANCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL   QSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA    EVLPEYG VKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EA+AAAPNSSTTRFVIYIKAGAYFENVEI RAK NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK DRNVVDGWTTFRSATVAV+GTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYR+CDIYGTIDFIFGNAAVVFQNCNLYARKPN NQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLAD 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL +
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGS 540
           P GWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEA+QFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGL 552
           SWLNSTGIPFFSGL
Sbjct: 541 SWLNSTGIPFFSGL 554

BLAST of Lsi02G024340 vs. NCBI nr
Match: XP_004136014.1 (pectinesterase isoform X2 [Cucumis sativus] >KGN45518.2 hypothetical protein Csa_015578 [Cucumis sativus])

HSP 1 Score: 1025.8 bits (2651), Expect = 1.4e-295
Identity = 513/558 (91.94%), Postives = 525/558 (94.09%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTSIST------FFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNL 60
           MYGL+NRAQI SNPTSIST      FFFIFFLFSFKPISSNPQ+H +ARSLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDC 120
           CFSKLSSFPQLASLSPEKLAGSALNFT+REVL AY NCT LKT LY GLNPTDR ALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180
           LELLDGSIAELKASIFDLA  QS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRAAAAG-EVLPEYGTVKGGFPKWVSVKDRRLLQAA 240
           ELYLGKIGQHLSIDLAMLKKIPGVNRA     E+LPEYG VKGGFPKWVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMF 300
           VNETKFN+VVAKDGSGNFTTV EA+AAAPN+STTRFVIYIKAGAYFENVEI RAK NLMF
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360
           VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQ 420
           DFSAFYQCSFIGYQDTLYVHSLRQFYRDCD+YGTIDFIFGNAAVVFQNCNLYARKPN NQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480
           RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480

Query: 481 QLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAF 540
           QL +P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEA+QFTVE F
Sbjct: 481 QLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVERF 540

Query: 541 IQGSSWLNSTGIPFFSGL 552
           IQGSSWLNST IPFFSGL
Sbjct: 541 IQGSSWLNSTEIPFFSGL 558

BLAST of Lsi02G024340 vs. NCBI nr
Match: XP_031744539.1 (pectinesterase isoform X1 [Cucumis sativus])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.3e-293
Identity = 513/564 (90.96%), Postives = 525/564 (93.09%), Query Frame = 0

Query: 1   MYGLKNRAQIPSNPTSIST------FFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNL 60
           MYGL+NRAQI SNPTSIST      FFFIFFLFSFKPISSNPQ+H +ARSLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDC 120
           CFSKLSSFPQLASLSPEKLAGSALNFT+REVL AY NCT LKT LY GLNPTDR ALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180
           LELLDGSIAELKASIFDLA  QS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRAAAAG-EVLPEYGTVKGGFPKWVSVKDRRLLQAA 240
           ELYLGKIGQHLSIDLAMLKKIPGVNRA     E+LPEYG VKGGFPKWVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMF 300
           VNETKFN+VVAKDGSGNFTTV EA+AAAPN+STTRFVIYIKAGAYFENVEI RAK NLMF
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKADRNVVDGWTTFRSAT------VAVVGTGFIAKGITFENYAGPSKHQAV 360
           VGDGIGKTLIKADRNVVDGWTTFRSAT      VAVVGTGFIAKGITFENYAGPSKHQAV
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVGFVYYVAVVGTGFIAKGITFENYAGPSKHQAV 360

Query: 361 ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYAR 420
           ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCD+YGTIDFIFGNAAVVFQNCNLYAR
Sbjct: 361 ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYAR 420

Query: 421 KPNPNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVF 480
           KPN NQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVF
Sbjct: 421 KPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVF 480

Query: 481 LRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQ 540
           LRSFIGQL +P GWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEA+Q
Sbjct: 481 LRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQ 540

Query: 541 FTVEAFIQGSSWLNSTGIPFFSGL 552
           FTVE FIQGSSWLNST IPFFSGL
Sbjct: 541 FTVERFIQGSSWLNSTEIPFFSGL 564

BLAST of Lsi02G024340 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 540.0 bits (1390), Expect = 2.2e-153
Identity = 285/520 (54.81%), Postives = 361/520 (69.42%), Query Frame = 0

Query: 40  HSARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVL 99
           H   SL    LF +  FS   +    +S    +L  +ALN T   V  + +N + L   L
Sbjct: 7   HIILSLTFLLLFLSTVFSSHVTQRFTSSDDITELVVTALNQTISNVNLSSSNFSDLLQRL 66

Query: 100 YGGLNPTDRRALDDCLELLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTC 159
              L+  D  A DDCLELLD ++ +L  +I  L   +S +   H++  L+SAAMTN RTC
Sbjct: 67  GSNLSHRDLCAFDDCLELLDDTVFDLTTAISKL---RSHSPELHNVKMLLSAAMTNTRTC 126

Query: 160 VDGFYNSSGT------------VRSRVELYLGKIGQHLSIDLAMLKKIPGVNRAAAAGEV 219
           +DGF +S               V   ++  L  I  H+S  LAML+ IPG       G+V
Sbjct: 127 LDGFASSDNDENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPG----HIPGKV 186

Query: 220 LPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTT 279
             +      GFP WVS  DR LLQ  V+ETK NLVVA++G+GN+TT+GEAI+AAPNSS T
Sbjct: 187 KEDV-----GFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTIGEAISAAPNSSET 246

Query: 280 RFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTG 339
           RFVIYIK G YFEN+EI R K  +MF+GDGIG+T+IKA+R+  DGWT F SATV V G+G
Sbjct: 247 RFVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWTAFHSATVGVRGSG 306

Query: 340 FIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGT 399
           FIAK ++F NYAGP KHQAVALRS+SD SA+Y+CSF  YQDT+YVHS +QFYR+CDIYGT
Sbjct: 307 FIAKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGT 366

Query: 400 IDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPV 459
           +DFIFG+A+VVFQNC+LYAR+PNPNQ+ I+TAQGRE+  + TGISI++ ++ AA DLIPV
Sbjct: 367 VDFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISIISSRILAAPDLIPV 426

Query: 460 LSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNT 519
            ++F+ YLGRPW+LYSRTV ++SFI  L DPAGWL+W   FAL+TLYYGEY+N GPGSN 
Sbjct: 427 QANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNM 486

Query: 520 TGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPF 548
           T RV WPG++ I    EA+QF+V  FI G+ WLNST IPF
Sbjct: 487 TNRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPF 514

BLAST of Lsi02G024340 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 512.3 bits (1318), Expect = 4.8e-145
Identity = 275/542 (50.74%), Postives = 349/542 (64.39%), Query Frame = 0

Query: 17  ISTFFFIFFLFSFKPISSNPQIHHSARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGS 76
           +S  FF+ FL +     S     H  + L      P L  +                   
Sbjct: 19  LSLIFFLIFLSTVVSSQSPSYTTHKTQRLTETKTIPELIIAD------------------ 78

Query: 77  ALNFTTREVLGAYANCTKLKTVLYGGLNPTDRRALDDCLELLDGSIAELKASIFDLALPQ 136
            LN T  +V  A +N + L+T L+  L   +R A +DCL LLD +I++L+ ++ DL   +
Sbjct: 79  -LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---R 138

Query: 137 SAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSID 196
           S++   +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  
Sbjct: 139 SSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNS 198

Query: 197 LAMLKKIPGVNRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGS 256
           L ML+ I     +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+
Sbjct: 199 LHMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGT 258

Query: 257 GNFTTVGEAIAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRN 316
           GNFTT+ +A+ AAPN S TRF+IYIK G YFENVE+ + K  +MF+GDGIGKT+IKA+R+
Sbjct: 259 GNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRS 318

Query: 317 VVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQD 376
            +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQD
Sbjct: 319 RIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQD 378

Query: 377 TLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQN 436
           TLYVHS +QFYR+CDIYGTIDFIFGNAAVVFQN +LYARKPNP  +  FTAQ R   +Q 
Sbjct: 379 TLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQP 438

Query: 437 TGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTF 496
           TGISILNC++ AA DLIPV  +F+ YLGRPW+ YSRTV ++SFI  L  PAGWLE    F
Sbjct: 439 TGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDF 498

Query: 497 ALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPFF 551
           AL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA QFTV  FI GS+WLNSTGIPF 
Sbjct: 499 ALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFS 531

BLAST of Lsi02G024340 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 492.7 bits (1267), Expect = 4.0e-139
Identity = 269/530 (50.75%), Postives = 339/530 (63.96%), Query Frame = 0

Query: 39  HHSARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTV 98
           H   +S+C+ TL+P LCFS +++       S +++  ++LN TT+ V   Y    KL   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-IA 118

Query: 99  LYGGLNPTDRRALDDCLELLDGSIAELKASIFDL---ALPQSAARHSHDLLTLVSAAMTN 158
              GL P +  AL DCLE +D ++ EL  ++ DL      +S  +H+ DL TL+S+A+TN
Sbjct: 119 KRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 159 HRTCVDGFYNSSGTVRSRVELYLGKIG-QHL-SIDLAMLKKIPGV--------------- 218
             TC+DGF       + R  L  G++  +H+ S  LAM+K +                  
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 219 -NRAAAAGEVLPEYGTVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEA 278
            N      EV  +  +   G+PKW+SV DRRLLQ +    K +  VA DGSG+FTTV  A
Sbjct: 239 NNNNRKLKEVTGDLDS--DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAA 298

Query: 279 IAAAPNSSTTRFVIYIKAGAYFENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFR 338
           +AAAP  S  RFVI+IKAG Y ENVE+ + K N+MF+GDG GKT+I   RNVVDG TTF 
Sbjct: 299 VAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFH 358

Query: 339 SATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQ 398
           SATVA VG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQ
Sbjct: 359 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQ 418

Query: 399 FYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCK 458
           F+  C I GT+DFIFGNAA V Q+C++ AR+PN  Q+N+ TAQGR DPNQNTGI I NC+
Sbjct: 419 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCR 478

Query: 459 VEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGE 518
           +   SDL+ V  +F TYLGRPWK YSRTV ++S I  +  P GW EW+G+FALDTL Y E
Sbjct: 479 IGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYRE 538

Query: 519 YLNRGPGSNTTGRVTWPGYRVITNATEANQFTVEAFIQGSSWLNSTGIPF 548
           YLNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 539 YLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Lsi02G024340 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 477.2 bits (1227), Expect = 1.7e-134
Identity = 252/518 (48.65%), Postives = 327/518 (63.13%), Query Frame = 0

Query: 46  CNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLNP 105
           C+ +L+PNLC   L  FP   +    +L   + N T ++   A    +   T+ Y  + P
Sbjct: 79  CSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS---TITYTQMPP 138

Query: 106 TDRRALDDCLELLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGF-- 165
             R A D CLELLD S+  L  ++  + +      HS D++T +S+AMTNH TC DGF  
Sbjct: 139 RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDTCTDGFDE 198

Query: 166 -YNSSGTVRSRVELYLGKIGQHLSIDLAM-------LKKIPGVNRAAAAGEVLPEYGTVK 225
                G V+ +V   +  + + +S  LA+       L  +P VN     G    E     
Sbjct: 199 IEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE----- 258

Query: 226 GGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIKA 285
              P W+  +DR LL    +  + ++ V+KDGSG F T+ EAI  AP  S+ RFVIY+KA
Sbjct: 259 -ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKA 318

Query: 286 GAY-FENVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGIT 345
           G Y  EN+++ R K NLMF+GDG GKT+I   +++ D  TTF +AT A  G GFI + +T
Sbjct: 319 GRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMT 378

Query: 346 FENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFGN 405
           FENYAGP+KHQAVALR   D +  Y+C+ IGYQD LYVHS RQF+R+C+IYGT+DFIFGN
Sbjct: 379 FENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGN 438

Query: 406 AAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTY 465
           AAV+ Q+CN+YARKP   Q+   TAQ R+DPNQNTGISI  CK+ A  DL     S+ TY
Sbjct: 439 AAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTY 498

Query: 466 LGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWP 525
           LGRPWKLYSR V++ S +G   DP GWLEWNG FALD+LYYGEY+N+G GS    RV WP
Sbjct: 499 LGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWP 558

Query: 526 GYRVITNATEANQFTVEAFIQGSSWLNSTGIPFFSGLA 553
           GY VIT+  EA++FTV  FI GSSWL STG+ FFSGL+
Sbjct: 559 GYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of Lsi02G024340 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 475.3 bits (1222), Expect = 6.6e-134
Identity = 256/519 (49.33%), Postives = 331/519 (63.78%), Query Frame = 0

Query: 46  CNGTLFPNLCFSKLSSFP-QLASLSPEKLAGSALNFTTREVLGAYANCTKLKTVLYGGLN 105
           C  T FP LC   L  FP  LA+ S + L    +N T      A  +   L  V    + 
Sbjct: 91  CELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFV---DMP 150

Query: 106 PTDRRALDDCLELLDGSIAELKASIFDLALPQSAARHSHDLLTLVSAAMTNHRTCVDGFY 165
           P  R A D C+ELLD S+  L  ++  +    S++    D+ T +SAA+TNH TC +GF 
Sbjct: 151 PRARSAYDSCVELLDDSVDALSRALSSVV---SSSAKPQDVTTWLSAALTNHDTCTEGFD 210

Query: 166 N-SSGTVRSRVELYLGKIGQHLSIDLAMLKK---------IPGVNRAAAAGEVLPEYGTV 225
               G V+  +   L  + + +S  LA+            +P  NR     E   E    
Sbjct: 211 GVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREE---- 270

Query: 226 KGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAIAAAPNSSTTRFVIYIK 285
              FP+W+  K+R +L+  V++ + +++V+KDG+G   T+ EAI  AP +ST R +IY+K
Sbjct: 271 --KFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVK 330

Query: 286 AGAYFE-NVEIERAKRNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGI 345
           AG Y E N+++ R K NLMFVGDG GKT+I   +++ D  TTF +A+ A  G GFIA+ I
Sbjct: 331 AGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDI 390

Query: 346 TFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDIYGTIDFIFG 405
           TFEN+AGP+KHQAVALR  +D +  Y+C+ IGYQDTLYVHS RQF+R+CDIYGT+DFIFG
Sbjct: 391 TFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFG 450

Query: 406 NAAVVFQNCNLYARKPNPNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRT 465
           NAAVV QNC++YARKP   Q+N  TAQ R+DPNQNTGISI   +V AASDL     S +T
Sbjct: 451 NAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQT 510

Query: 466 YLGRPWKLYSRTVFLRSFIGQLADPAGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTW 525
           YLGRPWKL+SRTV++ S+IG      GWLEWN TFALDTLYYGEYLN GPGS    RV+W
Sbjct: 511 YLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSW 570

Query: 526 PGYRVITNATEANQFTVEAFIQGSSWLNSTGIPFFSGLA 553
           PGYRVI +  EAN+FTV  FI GSSWL STG+ F +GL+
Sbjct: 571 PGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O813013.1e-15254.81Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
O814156.8e-14450.74Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
P850761.5e-14377.43Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
P839482.3e-13950.75Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O048868.6e-13950.94Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BSU38.5e-29993.50Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1[more]
A0A5D3D1W61.1e-29893.32Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130... [more]
A0A6J1JU831.3e-29189.75Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1[more]
A0A6J1GNU41.8e-28888.67Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1[more]
A0A0A0K5K81.2e-27691.76Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038896904.15.5e-30894.95pectinesterase-like [Benincasa hispida][more]
XP_008451545.21.8e-29893.50PREDICTED: pectinesterase-like [Cucumis melo][more]
KAA0046658.12.3e-29893.32pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [... [more]
XP_004136014.11.4e-29591.94pectinesterase isoform X2 [Cucumis sativus] >KGN45518.2 hypothetical protein Csa... [more]
XP_031744539.11.3e-29390.96pectinesterase isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G02320.12.2e-15354.81Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.14.8e-14550.74Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.14.0e-13950.75pectin methylesterase 2 [more]
AT5G53370.11.7e-13448.65pectin methylesterase PCR fragment F [more]
AT3G49220.16.6e-13449.33Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 37..191
e-value: 1.4E-27
score: 107.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 43..174
e-value: 7.1E-22
score: 78.4
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 17..194
e-value: 2.4E-22
score: 77.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 240..536
e-value: 1.8E-145
score: 483.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 41..197
e-value: 4.4E-26
score: 93.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 43..196
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 234..551
e-value: 1.7E-124
score: 417.3
NoneNo IPR availablePANTHERPTHR31707:SF257PECTINESTERASEcoord: 39..548
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 39..548
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 44..197
e-value: 8.95908E-35
score: 126.399
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 384..393
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 238..550

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G024340.1Lsi02G024340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity