Lsi02G022560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G022560
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionC2H2-type domain-containing protein
Locationchr02: 29097778 .. 29100956 (-)
RNA-Seq ExpressionLsi02G022560
SyntenyLsi02G022560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGCATTTCACCTCAAACCAAAAAAAAAAAAAGGATTTTTAATATGTTTGCTTCAACAATGTCCAATTCAACTTCTTTGTCTGAAGAAGCTAGTGTCTCTTCTACCACAAGAGTCCACCCTGATTTCACCTGCTTAAACCCTCTCCTTCCATCTTTTTCTGATATTCACCACTCCCCACAAAAGCCCAAGAAGAAACGAAGCCTCCCCGGTAACCCAGGTAACGTTCCTGTTTCCTGTTTCTTGTTTCTTGTTTTTTGTTCAATGTTTTTCGAGATTAACGATCGAGAATTATATACGTGTGTAGACCCGGATGCGGAAGTGATAACGCTTTCGCCGAAAACACTATTAGCAACAAATAGATTTGTGTGTGAGATTTGCAACAAGGGTTTCCAAAGGGATCAAAATTTGCAGTTGCATAGGAGAGGTCACAATTTACCATGGAAATTGAAGCAAAGAAACAACAAAGAAGTGAAGAAGAAAGCCTATGTTTGTCCAGAACCAAGTTGTGTTCATCATCATCCTTCAAGAGCATTAGGAGATCTCACAGGAATCAAAAAACATTATTGCAGAAAACATGGTGAAAAGAAATGGAAATGTGATAAATGTTCCAAAGTTTATGCTGTTCAATCTGATTGGAAAGCTCACTCCAAAACTTGTGGCACTAGGGAATATAGATGTGATTGTGGAACCCTTTTCTCCAGGTAATTTTTTTTTTCATTTAAAATTTCGATAATATCAAGAGAAATAGTACATGTCAATTATCGTTGAATTATGCTGACTATTTAGTTATTGAATTCTTGTTTGTACTCTTTCTTGTTTTTGTTGGAGGTAAGAGGTAACCCAAAAAGATTGGTCTTTTGTTTTGAATGGGAACCCTTTAGTGTCTTTAATTTTGGCAAAATTTTCAAAATTTAATTCCATTTTCTTTCACTAAAATGGTAACCCTTCTATGCATGGAAAATGTCTATACACAAATACATATCTTTGAATTTTCATTTTTTGGGGAGAAAAATGAAGTAATACAAAAAAAAGAAATATATTTAGTGGGTATGGTGTAGATTTATGGGAAATTTATGAAAACTTGATTTTAAAAAATATATTTGTTTTTTTTAGGGGTTTGAACTTTGAATTAAGGGTTTGTGTGTGATTTATGAGATTCCTTTGGAATGATTGGAAAAAGAGAACATTTTTGACTGATTTTTGTTATGTGGAGTAGAAGAATTGAAATTTTTTAATTTCAAAATCGATAATACAAATTTTACGTGTCGATCTCTATTTATATTGGTCATTTTTTTGCAGAAATTTGACTGAATTGATTGTGAAGAATTGGGATGGATGATGTTTAAGGATATTATGGATCCGTGACTACTTTGTATTATTATTTTGAGAGGGATATTGTTCATAAGAATTATTTGCTTGCAGAGCTCTGAAATTCTGAACCCTTCATCATTATGAAAACTGGATAAATCTGCAAGAAAATCTGATATTTCATCAGTTGCTGATTCTTTTTTTTTTTTTTTTTAATATATGTATATATATATATATATATATATATATATATATATTTTCTTGCTATTTGTTTCTTATTGATCTTATCTCTCTCTCTCTCTCTCTTTCTATTTTGATTCACAGAAGTAAAAAGAGAAATAAATGAAAGAAAGATTTAGAGTATTATTTTAGAGCCTTGTTTTTGTGTATGAATGAAAATGAATACATGACTCAGTATTCTTTGTTTTGCATGCATGTGCTACAGTACACTAAAGGAAATAAATAAATAAAATAAATATAAAATAAAAGTCTTATAAAGTTTACTGATTCGTGGGCTTCTTAGTCTCATTTGTTGCTCTACTTTTTATTTTGACCAAAAGAGAGAGAGTTTTTCATTTGGAACCATGTTTTTGCTTTTTGAGTCAATGTGAGATTTTTATGAACAATATGGTATGAATTTTCTTGAGCTCTTCACCAAAGTCCCACCATATATATGAAAACAAAACAAAACACAATCTAAATTTGTTGAAATTACACATATTATTCTTTTCATATAATTAATTAACTTCATGCAATGTAACACAATTACTTTTCAACAAACACTTTTTTTTTTTTTTTGCTTTTTTTTTTCTATTGTTTCTTTTTGCAATTCAATTATGTTTGGTGAATCTCTAATCATTGCATGGGAAATATATTATATGTGTGTAGGAAGGATAGCTTCATAACCCATAGAGCTTTTTGTGATGCATTGGCTGAAGAAAGTGCAAGGCTCTCAGCTAACCAATTGGAAATGGCTGCTGCCGCGGTGGCTGCTGCCTCGGGTGCCACTGACATCAACATAAACTCGTCCTCGACCACCGCCGCCCAATCGCTCTTTCTTTATGGTAATCACCATCAATTCTCAAACTCGTCATTATCACTACCACTACCACCACCACCTCTGTCGCAAACCCACATGTCTTTAAACCCTTGGGACCATCCCCAAAACCCTAACTCTAATCACACCCTCCAAATCATTAAGCCAGAGGATTCAAATTCTCATAATTTTCACCAAATTCCAAATTTGCCCCTCTCCAATCCCTCATCTTCCAACAAGAACAACCCTTTTGGTCTAATTCAAGACCACCACCAACAAAAGGCAGGCTCATCCTCCTCCTCTTCCATGATAACCTCACCCTTCAGGAACCTCCACGTGTCGGTGCACCCGGGATCAAATGCTGCCACGTCAGCTCACCTATCAGCCACAGCCCTACTCCAAAAGGCAGCAACAGTTGGCGTTGGCGCTGCAAAATCCGGTCACCATCAGCCCGAGTCAGTGGGTCACATGACTCACTTCAACATTCCCAGCCTCCCCGAGTTCGGGCCACCGAATCACATTGACTCACTGAGTCAACTCGATCCCGACTATGCCACATGGCAGAAGACTGACCGGCTGACCAGGGACTTTCTCGGTCTGACTGGAGATGGGAACAATGGCGTTAACGGTGGTGGTGGTTCTGGCGGCGGGGGAGTGGTGCAGGTTAGCATGAGCGTTAAAGATATGCTAACGTACGCAAGTGGGTTGGAGATGTTTAAGCCTCACAATAATAATAATAATAATAATAGTAATAATAATTTTGGATACGCGGAGCCAGCTGCCAATGAAACTTGGGGAGACTGTTGAATGAAACGCTGTCGTTTCACTAGAG

mRNA sequence

AAAGGCATTTCACCTCAAACCAAAAAAAAAAAAAGGATTTTTAATATGTTTGCTTCAACAATGTCCAATTCAACTTCTTTGTCTGAAGAAGCTAGTGTCTCTTCTACCACAAGAGTCCACCCTGATTTCACCTGCTTAAACCCTCTCCTTCCATCTTTTTCTGATATTCACCACTCCCCACAAAAGCCCAAGAAGAAACGAAGCCTCCCCGGTAACCCAGACCCGGATGCGGAAGTGATAACGCTTTCGCCGAAAACACTATTAGCAACAAATAGATTTGTGTGTGAGATTTGCAACAAGGGTTTCCAAAGGGATCAAAATTTGCAGTTGCATAGGAGAGGTCACAATTTACCATGGAAATTGAAGCAAAGAAACAACAAAGAAGTGAAGAAGAAAGCCTATGTTTGTCCAGAACCAAGTTGTGTTCATCATCATCCTTCAAGAGCATTAGGAGATCTCACAGGAATCAAAAAACATTATTGCAGAAAACATGGTGAAAAGAAATGGAAATGTGATAAATGTTCCAAAGTTTATGCTGTTCAATCTGATTGGAAAGCTCACTCCAAAACTTGTGGCACTAGGGAATATAGATGTGATTGTGGAACCCTTTTCTCCAGGAAGGATAGCTTCATAACCCATAGAGCTTTTTGTGATGCATTGGCTGAAGAAAGTGCAAGGCTCTCAGCTAACCAATTGGAAATGGCTGCTGCCGCGGTGGCTGCTGCCTCGGGTGCCACTGACATCAACATAAACTCGTCCTCGACCACCGCCGCCCAATCGCTCTTTCTTTATGGTAATCACCATCAATTCTCAAACTCGTCATTATCACTACCACTACCACCACCACCTCTGTCGCAAACCCACATGTCTTTAAACCCTTGGGACCATCCCCAAAACCCTAACTCTAATCACACCCTCCAAATCATTAAGCCAGAGGATTCAAATTCTCATAATTTTCACCAAATTCCAAATTTGCCCCTCTCCAATCCCTCATCTTCCAACAAGAACAACCCTTTTGGTCTAATTCAAGACCACCACCAACAAAAGGCAGGCTCATCCTCCTCCTCTTCCATGATAACCTCACCCTTCAGGAACCTCCACGTGTCGGTGCACCCGGGATCAAATGCTGCCACGTCAGCTCACCTATCAGCCACAGCCCTACTCCAAAAGGCAGCAACAGTTGGCGTTGGCGCTGCAAAATCCGGTCACCATCAGCCCGAGTCAGTGGGTCACATGACTCACTTCAACATTCCCAGCCTCCCCGAGTTCGGGCCACCGAATCACATTGACTCACTGAGTCAACTCGATCCCGACTATGCCACATGGCAGAAGACTGACCGGCTGACCAGGGACTTTCTCGGTCTGACTGGAGATGGGAACAATGGCGTTAACGGTGGTGGTGGTTCTGGCGGCGGGGGAGTGGTGCAGGTTAGCATGAGCGTTAAAGATATGCTAACGTACGCAAGTGGGTTGGAGATGTTTAAGCCTCACAATAATAATAATAATAATAATAGTAATAATAATTTTGGATACGCGGAGCCAGCTGCCAATGAAACTTGGGGAGACTGTTGAATGAAACGCTGTCGTTTCACTAGAG

Coding sequence (CDS)

ATGTTTGCTTCAACAATGTCCAATTCAACTTCTTTGTCTGAAGAAGCTAGTGTCTCTTCTACCACAAGAGTCCACCCTGATTTCACCTGCTTAAACCCTCTCCTTCCATCTTTTTCTGATATTCACCACTCCCCACAAAAGCCCAAGAAGAAACGAAGCCTCCCCGGTAACCCAGACCCGGATGCGGAAGTGATAACGCTTTCGCCGAAAACACTATTAGCAACAAATAGATTTGTGTGTGAGATTTGCAACAAGGGTTTCCAAAGGGATCAAAATTTGCAGTTGCATAGGAGAGGTCACAATTTACCATGGAAATTGAAGCAAAGAAACAACAAAGAAGTGAAGAAGAAAGCCTATGTTTGTCCAGAACCAAGTTGTGTTCATCATCATCCTTCAAGAGCATTAGGAGATCTCACAGGAATCAAAAAACATTATTGCAGAAAACATGGTGAAAAGAAATGGAAATGTGATAAATGTTCCAAAGTTTATGCTGTTCAATCTGATTGGAAAGCTCACTCCAAAACTTGTGGCACTAGGGAATATAGATGTGATTGTGGAACCCTTTTCTCCAGGAAGGATAGCTTCATAACCCATAGAGCTTTTTGTGATGCATTGGCTGAAGAAAGTGCAAGGCTCTCAGCTAACCAATTGGAAATGGCTGCTGCCGCGGTGGCTGCTGCCTCGGGTGCCACTGACATCAACATAAACTCGTCCTCGACCACCGCCGCCCAATCGCTCTTTCTTTATGGTAATCACCATCAATTCTCAAACTCGTCATTATCACTACCACTACCACCACCACCTCTGTCGCAAACCCACATGTCTTTAAACCCTTGGGACCATCCCCAAAACCCTAACTCTAATCACACCCTCCAAATCATTAAGCCAGAGGATTCAAATTCTCATAATTTTCACCAAATTCCAAATTTGCCCCTCTCCAATCCCTCATCTTCCAACAAGAACAACCCTTTTGGTCTAATTCAAGACCACCACCAACAAAAGGCAGGCTCATCCTCCTCCTCTTCCATGATAACCTCACCCTTCAGGAACCTCCACGTGTCGGTGCACCCGGGATCAAATGCTGCCACGTCAGCTCACCTATCAGCCACAGCCCTACTCCAAAAGGCAGCAACAGTTGGCGTTGGCGCTGCAAAATCCGGTCACCATCAGCCCGAGTCAGTGGGTCACATGACTCACTTCAACATTCCCAGCCTCCCCGAGTTCGGGCCACCGAATCACATTGACTCACTGAGTCAACTCGATCCCGACTATGCCACATGGCAGAAGACTGACCGGCTGACCAGGGACTTTCTCGGTCTGACTGGAGATGGGAACAATGGCGTTAACGGTGGTGGTGGTTCTGGCGGCGGGGGAGTGGTGCAGGTTAGCATGAGCGTTAAAGATATGCTAACGTACGCAAGTGGGTTGGAGATGTTTAAGCCTCACAATAATAATAATAATAATAATAGTAATAATAATTTTGGATACGCGGAGCCAGCTGCCAATGAAACTTGGGGAGACTGTTGA

Protein sequence

MFASTMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDPDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYVCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTREYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSSTTAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSNSHNFHQIPNLPLSNPSSSNKNNPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVSVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPNHIDSLSQLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLTYASGLEMFKPHNNNNNNNSNNNFGYAEPAANETWGDC
Homology
BLAST of Lsi02G022560 vs. ExPASy Swiss-Prot
Match: O22759 (Protein indeterminate-domain 12 OS=Arabidopsis thaliana OX=3702 GN=IDD12 PE=2 SV=2)

HSP 1 Score: 350.1 bits (897), Expect = 4.1e-95
Identity = 226/480 (47.08%), Postives = 264/480 (55.00%), Query Frame = 0

Query: 10  TSLSEEASVSS---TTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDPDAEVIT 69
           +SLS EAS SS   T     +F+  + ++ S    H    KPKKKR LPGNPDPDAEVI 
Sbjct: 12  SSLSTEASASSGNNTLSTIQEFSGFHNVISSVC-THTETHKPKKKRGLPGNPDPDAEVIA 71

Query: 70  LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKE-VKKKAYVCPEPS 129
           LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQ+N KE  KKK YVCPE +
Sbjct: 72  LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQKNTKEQQKKKVYVCPETN 131

Query: 130 CVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTREYRCDC 189
           C HHHPSRALGDLTGIKKH+CRKHGEKKWKC+KCSK YAVQSDWKAH+K CGTR+YRCDC
Sbjct: 132 CAHHHPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKFYAVQSDWKAHTKICGTRDYRCDC 191

Query: 190 GTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSSTTAAQS 249
           GTLFSRKD+FITHRAFCDALAEESARL +           ++S  T+ N N         
Sbjct: 192 GTLFSRKDTFITHRAFCDALAEESARLHS----------TSSSNLTNPNPN--------- 251

Query: 250 LFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSNSHNFH 309
              +  HH   N S SL     PL                        I+P         
Sbjct: 252 ---FQGHHFMFNKSSSLLFTSSPL-----------------------FIEP--------- 311

Query: 310 QIPNLPLSNPSSSNKNNPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVSVHPGSNAATSA 369
                                          S S++++ T P                +A
Sbjct: 312 -------------------------------SLSTAALSTPP----------------TA 371

Query: 370 HLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPNHIDSLSQLDPDY- 429
            LSATALLQKA ++       G  Q  S+GH  H ++ ++ EF   + +   S    +Y 
Sbjct: 372 ALSATALLQKATSLS-STTFGGGGQTRSIGH--HRHLTNVNEFLGVDRVMMTSASSSEYD 371

Query: 430 --------ATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLTYASGL 477
                   +TWQK DRLTRDFLGLTG G +               VS+   DML YA G+
Sbjct: 432 QLVVDGFTSTWQKADRLTRDFLGLTGHGGH---------------VSVRPGDMLEYAGGV 371

BLAST of Lsi02G022560 vs. ExPASy Swiss-Prot
Match: Q9ZWA6 (Zinc finger protein MAGPIE OS=Arabidopsis thaliana OX=3702 GN=MGP PE=1 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 9.8e-89
Identity = 201/389 (51.67%), Postives = 240/389 (61.70%), Query Frame = 0

Query: 40  DIHHSPQKP--KKKRSLPGNPDPDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHR 99
           D H S   P  KKKR+LPGNPDP+AEVI LSPKTL+ATNRF+CEIC KGFQRDQNLQLHR
Sbjct: 30  DQHESLNPPLVKKKRNLPGNPDPEAEVIALSPKTLMATNRFLCEICGKGFQRDQNLQLHR 89

Query: 100 RGHNLPWKLKQRNNKEVKKKAYVCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCD 159
           RGHNLPWKLKQR +KEV+K+ YVCPE SCVHHHP+RALGDLTGIKKH+CRKHGEKKWKC+
Sbjct: 90  RGHNLPWKLKQRTSKEVRKRVYVCPEKSCVHHHPTRALGDLTGIKKHFCRKHGEKKWKCE 149

Query: 160 KCSKVYAVQSDWKAHSKTCGTREYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQL 219
           KC+K YAVQSDWKAHSKTCGTREYRCDCGT+FSR+DSFITHRAFCDALAEE+ARL+A   
Sbjct: 150 KCAKRYAVQSDWKAHSKTCGTREYRCDCGTIFSRRDSFITHRAFCDALAEETARLNAAS- 209

Query: 220 EMAAAAVAAASGATDININSSSTTAAQSLFLYGNHHQFSNS---SLSLPLPP-----PPL 279
                           ++ S + TA  +L    N+H    +   S SLP PP     PP 
Sbjct: 210 ----------------HLKSFAATAGSNL----NYHYLMGTLIPSPSLPQPPSFPFGPPQ 269

Query: 280 SQTH------MSLNPWDHPQNPNSNHTLQIIKPEDSNSHNFHQIPNLPLSNPSSSNK--N 339
            Q H      ++ N +DH        TL +    + N H    I +     P S  +  N
Sbjct: 270 PQHHHHHQFPITTNNFDHQDVMKPASTLSLWSGGNINHHQQVTIEDRMAPQPHSPQEDYN 329

Query: 340 NPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVSVHPGSNAATS----------------- 387
             FG   +H +     ++S S+IT       V     +N ATS                 
Sbjct: 330 WVFGNANNHGEL---ITTSDSLITHDNNINIVQSKENANGATSLSVPSLFSSVDQITQDA 389

BLAST of Lsi02G022560 vs. ExPASy Swiss-Prot
Match: Q8H1F5 (Protein indeterminate-domain 7 OS=Arabidopsis thaliana OX=3702 GN=IDD7 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.0e-85
Identity = 177/307 (57.65%), Postives = 213/307 (69.38%), Query Frame = 0

Query: 6   MSNSTSLS-EEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQ------KPKKKRSLPGNP 65
           MSN TS S ++ASVSS  R     + +N         HH  Q        K+KR+ PGNP
Sbjct: 21  MSNLTSASGDQASVSSGNRTETSGSNINQ--------HHQEQCFVPQSSLKRKRNQPGNP 80

Query: 66  DPDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKE-VKKK 125
           DP+AEV+ LSPKTL+ATNRF+CE+CNKGFQRDQNLQLH+RGHNLPWKLKQR+NK+ V+KK
Sbjct: 81  DPEAEVMALSPKTLMATNRFICEVCNKGFQRDQNLQLHKRGHNLPWKLKQRSNKDVVRKK 140

Query: 126 AYVCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCG 185
            YVCPEP CVHHHPSRALGDLTGIKKH+ RKHGEKKWKC+KCSK YAVQSDWKAH+KTCG
Sbjct: 141 VYVCPEPGCVHHHPSRALGDLTGIKKHFFRKHGEKKWKCEKCSKKYAVQSDWKAHAKTCG 200

Query: 186 TREYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAA-AVAAASGATDININ 245
           T+EY+CDCGTLFSR+DSFITHRAFCDALAEESAR   N + + A+ +       T  NI 
Sbjct: 201 TKEYKCDCGTLFSRRDSFITHRAFCDALAEESARAMPNPIMIQASNSPHHHHHQTQQNIG 260

Query: 246 SSSTTAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKP 304
            SS+          + +  SNS+L  P+        + ++ PW    NPN N     + P
Sbjct: 261 FSSS----------SQNIISNSNLHGPMKQEESQHHYQNIPPWLISSNPNPNGNNGNLFP 309

BLAST of Lsi02G022560 vs. ExPASy Swiss-Prot
Match: Q8GYC1 (Protein indeterminate-domain 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IDD4 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.1e-84
Identity = 210/508 (41.34%), Postives = 280/508 (55.12%), Query Frame = 0

Query: 4   STMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDPDAE 63
           S + +S+S ++   ++S+     DF   +  +      + S   PKK+R+ PGNP+PDAE
Sbjct: 9   SVIPSSSSSAQPFFITSSGTGDNDFNRKDTFMSMIQQPNSSAPPPKKRRNQPGNPNPDAE 68

Query: 64  VITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYVCPE 123
           V+ LSPKTL+ATNRF+C++CNKGFQR+QNLQLHRRGHNLPWKLKQ++ KEVK+K Y+CPE
Sbjct: 69  VVALSPKTLMATNRFICDVCNKGFQREQNLQLHRRGHNLPWKLKQKSTKEVKRKVYLCPE 128

Query: 124 PSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTREYRC 183
           P+CVHH PSRALGDLTGIKKHY RKHGEKKWKC+KCSK YAVQSDWKAHSKTCGT+EYRC
Sbjct: 129 PTCVHHDPSRALGDLTGIKKHYYRKHGEKKWKCEKCSKRYAVQSDWKAHSKTCGTKEYRC 188

Query: 184 DCGTLFSRKDSFITHRAFCDALAEESAR-LSANQLEMAAAAVAAASGATDININSSSTTA 243
           DCGT+FSR+DS+ITHRAFCDAL +E+AR  + +   M AA+    SG     +   S  +
Sbjct: 189 DCGTIFSRRDSYITHRAFCDALIQETARNPTVSFTSMTAASSGVGSGGIYGRLGGGSALS 248

Query: 244 AQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDH-----PQNPNSNHTLQIIKPE 303
                   +HH   + +        PL   ++++   D+     PQ+ N N  +Q    +
Sbjct: 249 --------HHHLSDHPNFGF----NPLVGYNLNIASSDNRRDFIPQSSNPNFLIQSASSQ 308

Query: 304 D-------------SNSHNFHQIPNLPLSNPSSSNKNNPFGLIQDHHQQKAGSSSSSSMI 363
                          N H   Q   +   N  SS  NN F  +    +    S +S   +
Sbjct: 309 GMLNTTPNNNNQSFMNQHGLIQFDPVDNINLKSSGTNNSFFNLGFFQENTKNSETSLPSL 368

Query: 364 TSPFRNLHVSVHPGSNAATSAHLSATALLQKAATVGV-----------GAAKSGHHQPES 423
            S    +H   H   N    +++SATALLQKA  +G            G A S +     
Sbjct: 369 YSTDVLVH---HREENLNAGSNVSATALLQKATQMGSVTSNDPSALFRGLASSSNSSSVI 428

Query: 424 VGHMTHFNIPSLPEFGP-PNHIDSLSQLD--------------PDYATWQKTDRLTRDFL 467
             H     I      G     ++SL+ ++               D      +D+LT DFL
Sbjct: 429 ANHFGGGRIMENDNNGNLQGLMNSLAAVNGGGGSGGSIFDVQFGDNGNMSGSDKLTLDFL 488

BLAST of Lsi02G022560 vs. ExPASy Swiss-Prot
Match: Q9SCQ6 (Zinc finger protein GAI-ASSOCIATED FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN=GAF1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.2e-83
Identity = 217/468 (46.37%), Postives = 269/468 (57.48%), Query Frame = 0

Query: 6   MSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDPDAEVI 65
           + NS+++S EASVS ++  +      NPL  S           KKKR+LPG PDP++EVI
Sbjct: 5   LDNSSTVSGEASVSISSTGNQ-----NPLPNSTG---------KKKRNLPGMPDPESEVI 64

Query: 66  TLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYVCPEPS 125
            LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKL+Q++NKEVKKK YVCPE S
Sbjct: 65  ALSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQKSNKEVKKKVYVCPEVS 124

Query: 126 CVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTREYRCDC 185
           CVHH PSRALGDLTGIKKH+CRKHGEKKWKCDKCSK YAVQSDWKAHSK CGT+EY+CDC
Sbjct: 125 CVHHDPSRALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHSKICGTKEYKCDC 184

Query: 186 GTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSSTTAAQS 245
           GTLFSR+DSFITHRAFCDALAEE+AR   +Q +     +          + +   T +  
Sbjct: 185 GTLFSRRDSFITHRAFCDALAEENARSHHSQSKKQNPEILTRKNPVPNPVPAPVDTESAK 244

Query: 246 LFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSNSHNFH 305
           +         S+S+L+       + Q+     P +  Q      +L ++      +  F 
Sbjct: 245 I--------KSSSTLT-------IKQSESPKTPPEIVQEAPKPTSLNVVTSNGVFAGLFE 304

Query: 306 QIPNLPLSNPSSSNKNNPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVSVHPGSNA-ATS 365
                P    +SS+  + F            SSSS   I+      H S   GSN     
Sbjct: 305 SSSASPSIYTTSSSSKSLF-----------ASSSSIEPISLGLSTSHGSSFLGSNRFHAQ 364

Query: 366 AHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFN------IPSLPEFGPP---NHID 425
             +SATALLQKAA   +GAA SG      +G ++  +      +P     G P       
Sbjct: 365 PAMSATALLQKAAQ--MGAASSGGSLLHGLGIVSSTSTSIDAIVPHGLGLGLPCGGESSS 424

Query: 426 SLSQLDPDYATWQKTDRLTRDFLGL---TGDGNNGVNG-----GGGSG 456
            L +L    ++     + T DFLGL    G+GN   NG     GGG+G
Sbjct: 425 GLKELMMGNSSVFGPKQTTLDFLGLGRAVGNGNGPSNGLSTLVGGGTG 430

BLAST of Lsi02G022560 vs. ExPASy TrEMBL
Match: A0A0A0K7A5 (C2H2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G387730 PE=4 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.2e-217
Identity = 408/479 (85.18%), Postives = 425/479 (88.73%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEE-ASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPD 60
           MFA+TMSNSTSLSEE ASVSSTTR+HPDFT LNPLLPSFSDIHH PQKPKKKRSLPGNPD
Sbjct: 1   MFAATMSNSTSLSEEAASVSSTTRIHPDFTSLNPLLPSFSDIHHPPQKPKKKRSLPGNPD 60

Query: 61  PDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY 120
           PDA+VI LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY
Sbjct: 61  PDADVIALSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY 120

Query: 121 VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR 180
           VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR
Sbjct: 121 VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR 180

Query: 181 EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAA-AVAAASGATDININSS 240
           EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQL MAAA A AA SGATD+NINSS
Sbjct: 181 EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLAMAAAVAAAAVSGATDVNINSS 240

Query: 241 STTAA-QSLFLYGN-HHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNS-NHTLQIIK 300
           S TAA QSLF YGN HHQ+S+ SLS     P  SQTH+SLNPW+H QNPN+ NH LQIIK
Sbjct: 241 SATAAVQSLFPYGNHHHQYSSPSLS---SLPMASQTHVSLNPWEHHQNPNNPNHNLQIIK 300

Query: 301 PEDS------NSHNFHQIPNLPLSNPSSS--NKNNPFGLIQDH-----HQQKAGSSSSSS 360
           PEDS      NSHNFHQIPN  LSN +SS  N NNPFGLIQ+H      QQK  SSS+SS
Sbjct: 301 PEDSNSHPPHNSHNFHQIPNFALSNSASSNNNNNNPFGLIQEHQHHHQQQQKVPSSSTSS 360

Query: 361 MITSPFRNLHVSVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNI 420
           MITSPFRNLHVSVH GSNAATSAHLSATALLQKAATVGV A+KSGHHQ ES GH+THFN+
Sbjct: 361 MITSPFRNLHVSVHQGSNAATSAHLSATALLQKAATVGVSASKSGHHQAESAGHITHFNM 420

Query: 421 PSLPEFGPPNHIDSLSQLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQ 462
           P++PEFGPPNHIDSLSQL PDYATWQKTDRLTRDFLGLTGDGN G   GGGS G GVVQ
Sbjct: 421 PNIPEFGPPNHIDSLSQLGPDYATWQKTDRLTRDFLGLTGDGNGG--AGGGSAGDGVVQ 474

BLAST of Lsi02G022560 vs. ExPASy TrEMBL
Match: A0A6J1H8L3 (protein indeterminate-domain 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460644 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 2.1e-206
Identity = 396/524 (75.57%), Postives = 427/524 (81.49%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDP 60
           MFA+TMSNSTSLSEEA+VS           LN LLP        PQK KKKRSLPGNPDP
Sbjct: 1   MFATTMSNSTSLSEEATVS-----------LNSLLP--------PQKIKKKRSLPGNPDP 60

Query: 61  DAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV 120
           DAEVI LSP+TLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRN+KEVKKKAYV
Sbjct: 61  DAEVIALSPRTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNSKEVKKKAYV 120

Query: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE 180
           CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKC+KCSKVYAVQSDWKAHSKTCGTRE
Sbjct: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCEKCSKVYAVQSDWKAHSKTCGTRE 180

Query: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSST 240
           YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQ  +AA   A A G       S+++
Sbjct: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQFVVAAEVEAVAEGGA---ATSTAS 240

Query: 241 TAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSN 300
            +AQSLF +GN +QF +SSLS P       QT+MSLNPWD+PQNPN N T QIIKPEDS+
Sbjct: 241 ASAQSLFPFGNLNQFPSSSLSRP------PQTYMSLNPWDNPQNPNPNQTHQIIKPEDSH 300

Query: 301 SHNFHQIPNLPLSNPS-SSNKNNPFGLIQDHHQ-------QKAGSSSSSSMITSPFRNLH 360
           SH+FHQIP LPL+  S S+  NNPFG+IQDHH+       QKAGSSSSSSMI SPFRNLH
Sbjct: 301 SHHFHQIPTLPLAGSSPSTATNNPFGIIQDHHRRHHPHHHQKAGSSSSSSMIASPFRNLH 360

Query: 361 VSVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPN 420
           VSV+P    A SAHLSATALLQKAATVGVGA KS  HQPESVGHMTHFN+P +PEF  P 
Sbjct: 361 VSVNP----AMSAHLSATALLQKAATVGVGAVKSSQHQPESVGHMTHFNMPDIPEFAAPT 420

Query: 421 HIDSLS-QLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLT 480
           H+DSL+ QL PDYATWQKTDRLTRDFLGLTGDGN      GG GG G +QVSMSVKDMLT
Sbjct: 421 HMDSLTHQLGPDYATWQKTDRLTRDFLGLTGDGN------GGGGGSGAMQVSMSVKDMLT 480

Query: 481 YASGLEMFKPHNNNNNNNSNNN-------FGYAEPAANETWGDC 509
           YA GLE+FKPHNNNNNNN+NN+       FG+AEPAANETW DC
Sbjct: 481 YAGGLEIFKPHNNNNNNNNNNSNNNNHSAFGFAEPAANETW-DC 485

BLAST of Lsi02G022560 vs. ExPASy TrEMBL
Match: A0A6J1GN29 (protein indeterminate-domain 9-like OS=Cucurbita moschata OX=3662 GN=LOC111455911 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 5.1e-205
Identity = 389/515 (75.53%), Postives = 425/515 (82.52%), Query Frame = 0

Query: 1   MFAST-MSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPD 60
           MF+ T MSNSTSLS++A++                   FS+IHHSPQK KKKRSLPGNPD
Sbjct: 30  MFSPTIMSNSTSLSDQATI-------------------FSNIHHSPQKLKKKRSLPGNPD 89

Query: 61  PDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY 120
           PDAEVI LSP TLLATNRFVCEICNKGFQRDQNLQLHRRGH LPWKLKQR++KE+KKKAY
Sbjct: 90  PDAEVIALSPTTLLATNRFVCEICNKGFQRDQNLQLHRRGHKLPWKLKQRSSKEMKKKAY 149

Query: 121 VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR 180
           VCPE  CVHH+PSRALGDLTGIKKHY RKHGEKKWKC KCSK+YAVQSDWKAHSKTCGTR
Sbjct: 150 VCPEEGCVHHNPSRALGDLTGIKKHYSRKHGEKKWKCLKCSKLYAVQSDWKAHSKTCGTR 209

Query: 181 EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSS 240
           EYRCDCGTLFSRKD F+THRAFCDALAEESARLSANQL +A    +AASGAT ++++ SS
Sbjct: 210 EYRCDCGTLFSRKDRFLTHRAFCDALAEESARLSANQLAIA----SAASGATGVDVSLSS 269

Query: 241 TTAAQSLFLYGNHHQFSNSSLSLPLPPPPL----SQTHMSLNPWDHPQNPNSNHTLQIIK 300
           TT AQSLF +GNH+QFSNS   +P  P  L    SQT +SLNPW+HP NPN NHTLQ+IK
Sbjct: 270 TTVAQSLFPFGNHYQFSNS--LIPSSPSSLSLSSSQTLVSLNPWNHPLNPNPNHTLQMIK 329

Query: 301 PEDSNSHNFHQIPNLPLSNPSSSNKN-NPFGLIQDHHQQ-KAGSSSSSSMITSPFRNLHV 360
           PEDSNSH+F QIPNLPLS+ SS+N N +PFGLIQDHH + ++ SSSSSSMITSPFRNLHV
Sbjct: 330 PEDSNSHHFQQIPNLPLSDDSSNNNNYHPFGLIQDHHHKARSSSSSSSSMITSPFRNLHV 389

Query: 361 SVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPNH 420
           SV P SN ATSAHLSATALLQKAATVG+ AAKSGHHQPESVGH+THFNI   PEF P NH
Sbjct: 390 SVQPASNVATSAHLSATALLQKAATVGIDAAKSGHHQPESVGHITHFNI---PEFRPSNH 449

Query: 421 IDSLSQLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLTYA 480
           I SLSQL PDYATWQK+DRLTRDFLGLTG GN     G GSG GG V VSM VKDMLTYA
Sbjct: 450 ISSLSQLGPDYATWQKSDRLTRDFLGLTGHGN---GTGSGSGSGGSVHVSMIVKDMLTYA 509

Query: 481 SGLEMFKPHNNNNNNNSNNNFGYAEPAANETWGDC 509
            G+EMFKPHNNNNN   +N FGY E AAN TWGDC
Sbjct: 510 GGIEMFKPHNNNNN---SNGFGYGEAAANGTWGDC 510

BLAST of Lsi02G022560 vs. ExPASy TrEMBL
Match: A0A6J1KRQ3 (protein indeterminate-domain 12-like OS=Cucurbita maxima OX=3661 GN=LOC111497661 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 2.5e-204
Identity = 395/519 (76.11%), Postives = 423/519 (81.50%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDP 60
           MFA+TMSNSTSLSEE +VS           LN LLP        PQK KKKRSLPGNPDP
Sbjct: 1   MFATTMSNSTSLSEEVTVS-----------LNSLLP--------PQKIKKKRSLPGNPDP 60

Query: 61  DAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV 120
           DAEVI LSP+TLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRN+KEVKKKAYV
Sbjct: 61  DAEVIALSPRTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNSKEVKKKAYV 120

Query: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE 180
           CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKC+KCSKVYAVQSDWKAHSKTCGTRE
Sbjct: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCEKCSKVYAVQSDWKAHSKTCGTRE 180

Query: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSST 240
           YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQ  +A    AA  GA          
Sbjct: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQFVIAPEVEAAEVGA---------A 240

Query: 241 TAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSN 300
           T+AQSLF +G+H+QF +SSLS         QT+MSLNPWD+PQNPN N T QIIKPEDS+
Sbjct: 241 TSAQSLFPFGSHNQFPSSSLSRS------PQTYMSLNPWDNPQNPNPNQTHQIIKPEDSH 300

Query: 301 SHNFHQIPNLPLSNPSSSN-KNNPFGLIQDHHQ------QKAGSSSSSSMITSPFRNLHV 360
           SH+FHQIP LPL+  S SN  NNPFG+IQDHH+      QKA SSSSSSMI +PFRNLHV
Sbjct: 301 SHHFHQIPALPLAGSSPSNATNNPFGIIQDHHRRHHHHHQKAASSSSSSMIATPFRNLHV 360

Query: 361 SVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPNH 420
           SV+P    A SAHLSATALLQKAATVGVGA KS  HQPESVGHMTHFN+P + EF  P H
Sbjct: 361 SVNP----AMSAHLSATALLQKAATVGVGAVKSSQHQPESVGHMTHFNMPDIAEFAAPTH 420

Query: 421 IDSLS-QLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLTY 480
           +DSL+ QL PDYATWQKTDRLTRDFLGLTGDGN    GGGGSGG   +QVSMSVKDMLTY
Sbjct: 421 MDSLTHQLGPDYATWQKTDRLTRDFLGLTGDGN---GGGGGSGG---MQVSMSVKDMLTY 474

Query: 481 ASGLEMFKPHNNNNNNNSNNN---FGYAEPAANETWGDC 509
           A GLE+FKPHNNNNNNN+NNN   FG+AEPAANETW DC
Sbjct: 481 AGGLEIFKPHNNNNNNNNNNNHSAFGFAEPAANETW-DC 474

BLAST of Lsi02G022560 vs. ExPASy TrEMBL
Match: A0A6J1JNM0 (protein indeterminate-domain 9-like OS=Cucurbita maxima OX=3661 GN=LOC111488512 PE=4 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.5e-196
Identity = 385/517 (74.47%), Postives = 417/517 (80.66%), Query Frame = 0

Query: 1   MFAST-MSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPD 60
           MF+ T MSNSTSLS++A++                   FS+IHH PQK KKKRSLPGNPD
Sbjct: 1   MFSPTIMSNSTSLSDQATI-------------------FSNIHHPPQKLKKKRSLPGNPD 60

Query: 61  PDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY 120
           PDAEVI LSP TLLATNRFVCEICNKGFQRDQNLQLHRRGH LPWKLKQR++KEVKKKAY
Sbjct: 61  PDAEVIALSPTTLLATNRFVCEICNKGFQRDQNLQLHRRGHKLPWKLKQRSSKEVKKKAY 120

Query: 121 VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR 180
           VCPE +CVHH+PSRALGDLTGIKKHY RKHGEKKWKC KCSK+YAVQSDWKAHSKTCGTR
Sbjct: 121 VCPEEACVHHNPSRALGDLTGIKKHYSRKHGEKKWKCLKCSKLYAVQSDWKAHSKTCGTR 180

Query: 181 EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSS 240
           EYRCDCGTLFSRKD F+THRAFCDALAEESARLSANQL +A    +AASGA  +++N SS
Sbjct: 181 EYRCDCGTLFSRKDRFVTHRAFCDALAEESARLSANQLAIA----SAASGAMGVDVNLSS 240

Query: 241 TTAAQSLFLYGNHHQFSNSSLSLPLPPPPL----SQTHMSLNPWDHPQNPNSNHTLQIIK 300
           TT AQSLF +GNH+QFSNS   +P  P  L    SQT +SLNPW+HP NP  NHTLQ+IK
Sbjct: 241 TTVAQSLFPFGNHYQFSNS--LIPSSPSSLSLSSSQTLVSLNPWNHPLNP--NHTLQMIK 300

Query: 301 PEDSNSHNFHQIPNLPLSNPSSSNKN-NPFGLIQDHHQQ-KAGSSSSSSMITSPFRNLHV 360
           PEDSNSH+FHQIPNLPLS+ SS+N N +PFGLIQDHH +  + SSSSSSMITSPFRNLHV
Sbjct: 301 PEDSNSHHFHQIPNLPLSDDSSNNNNYHPFGLIQDHHHKASSSSSSSSSMITSPFRNLHV 360

Query: 361 SVHPGSNAATSAHLSATALLQKAAT--VGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPP 420
           SV P SNAATSAHLSATALLQKAAT  VGVGAAKSG HQPES GH+THFNI   PEF P 
Sbjct: 361 SVQPASNAATSAHLSATALLQKAATVGVGVGAAKSGQHQPESAGHITHFNI---PEFRPS 420

Query: 421 NHIDSLSQLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLT 480
           NHI SLSQL PDYATWQK+DRLTRDFLGLTG GN     G GSG GG V  SM VKDML 
Sbjct: 421 NHISSLSQLGPDYATWQKSDRLTRDFLGLTGHGN---GTGSGSGSGGSVHFSMIVKDMLM 478

Query: 481 YASGLEMFKPHNNNNNNNSNNNFGYAEPAANETWGDC 509
           YA G    KPH  NNNNNS+N FGY E AAN TW DC
Sbjct: 481 YADG----KPH--NNNNNSSNGFGYGEAAANGTWEDC 478

BLAST of Lsi02G022560 vs. NCBI nr
Match: XP_038897540.1 (zinc finger protein GAI-ASSOCIATED FACTOR 1-like [Benincasa hispida])

HSP 1 Score: 897.5 bits (2318), Expect = 5.3e-257
Identity = 461/516 (89.34%), Postives = 474/516 (91.86%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDP 60
           MFA+TMSNSTSLSEEASVSSTTR+HPDFT LNPLLPSFSDIHH PQK KKKRSLPGNPDP
Sbjct: 1   MFAATMSNSTSLSEEASVSSTTRIHPDFTSLNPLLPSFSDIHHPPQKVKKKRSLPGNPDP 60

Query: 61  DAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV 120
           DAEVI LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV
Sbjct: 61  DAEVIVLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV 120

Query: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE 180
           CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE
Sbjct: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE 180

Query: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSST 240
           YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQL MAAAA        DININSSST
Sbjct: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLAMAAAA--------DININSSST 240

Query: 241 TAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSN 300
           T+AQSLF Y NHHQFSNSSLSL   P PLSQTHMSLNPWDH QNPN NHTLQIIKPE+SN
Sbjct: 241 TSAQSLFPYANHHQFSNSSLSL---PTPLSQTHMSLNPWDHLQNPNPNHTLQIIKPEESN 300

Query: 301 -----SHNFHQIPNLPLSNPSSS-NKNNPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVS 360
                SHNFHQIPN PLSN SSS N NNPFGLIQDHH Q+AGSSSSSSMITSPFRNLHVS
Sbjct: 301 SHTSHSHNFHQIPNWPLSNSSSSNNNNNPFGLIQDHHHQRAGSSSSSSMITSPFRNLHVS 360

Query: 361 VHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPNHI 420
           VHPG NAATSAHLSATALLQKAATVGVGA+KSGHHQPESVGH+THFN+P++PEFGPPN I
Sbjct: 361 VHPGLNAATSAHLSATALLQKAATVGVGASKSGHHQPESVGHITHFNMPNIPEFGPPNQI 420

Query: 421 DSLSQLDPDYATWQKTDRLTRDFLGLTGDGNNGVNG-GGGSGGGGVVQVSMSVKDMLTYA 480
           DSLSQL PDY TWQKTDRLTRDFLGLTGDGN GV G  GGSGGGGVVQVSM VKDML+YA
Sbjct: 421 DSLSQLGPDYTTWQKTDRLTRDFLGLTGDGNGGVGGTDGGSGGGGVVQVSMRVKDMLSYA 480

Query: 481 SGLEMFKPHNNNNN-NNSNNNFGYAEPAANETWGDC 509
            G+EMFKPHNNNNN NN+NNNFGYAEPAANETWGDC
Sbjct: 481 GGMEMFKPHNNNNNSNNNNNNFGYAEPAANETWGDC 505

BLAST of Lsi02G022560 vs. NCBI nr
Match: XP_011659290.1 (zinc finger protein BALDIBIS [Cucumis sativus] >KAE8646309.1 hypothetical protein Csa_016902 [Cucumis sativus])

HSP 1 Score: 840.5 bits (2170), Expect = 7.7e-240
Identity = 447/530 (84.34%), Postives = 467/530 (88.11%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEE-ASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPD 60
           MFA+TMSNSTSLSEE ASVSSTTR+HPDFT LNPLLPSFSDIHH PQKPKKKRSLPGNPD
Sbjct: 1   MFAATMSNSTSLSEEAASVSSTTRIHPDFTSLNPLLPSFSDIHHPPQKPKKKRSLPGNPD 60

Query: 61  PDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY 120
           PDA+VI LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY
Sbjct: 61  PDADVIALSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAY 120

Query: 121 VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR 180
           VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR
Sbjct: 121 VCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTR 180

Query: 181 EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAA-AVAAASGATDININSS 240
           EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQL MAAA A AA SGATD+NINSS
Sbjct: 181 EYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLAMAAAVAAAAVSGATDVNINSS 240

Query: 241 STTAA-QSLFLYGN-HHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNS-NHTLQIIK 300
           S TAA QSLF YGN HHQ+S+ SLS     P  SQTH+SLNPW+H QNPN+ NH LQIIK
Sbjct: 241 SATAAVQSLFPYGNHHHQYSSPSLS---SLPMASQTHVSLNPWEHHQNPNNPNHNLQIIK 300

Query: 301 PEDS------NSHNFHQIPNLPLSNPSSS--NKNNPFGLIQDH-----HQQKAGSSSSSS 360
           PEDS      NSHNFHQIPN  LSN +SS  N NNPFGLIQ+H      QQK  SSS+SS
Sbjct: 301 PEDSNSHPPHNSHNFHQIPNFALSNSASSNNNNNNPFGLIQEHQHHHQQQQKVPSSSTSS 360

Query: 361 MITSPFRNLHVSVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNI 420
           MITSPFRNLHVSVH GSNAATSAHLSATALLQKAATVGV A+KSGHHQ ES GH+THFN+
Sbjct: 361 MITSPFRNLHVSVHQGSNAATSAHLSATALLQKAATVGVSASKSGHHQAESAGHITHFNM 420

Query: 421 PSLPEFGPPNHIDSLSQLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQV 480
           P++PEFGPPNHIDSLSQL PDYATWQKTDRLTRDFLGLTGDGN G   GGGS G GVVQV
Sbjct: 421 PNIPEFGPPNHIDSLSQLGPDYATWQKTDRLTRDFLGLTGDGNGG--AGGGSAGDGVVQV 480

Query: 481 SMSVKDMLTYASGLEMFKPHNNN----NNNNSNNNFGYAEPAANETWGDC 509
           SMSVKDML+YA GLEMFK HNNN    NNNN++NNFGY EP ANE WGDC
Sbjct: 481 SMSVKDMLSYAGGLEMFKRHNNNNNNTNNNNNSNNFGYGEPPANEAWGDC 525

BLAST of Lsi02G022560 vs. NCBI nr
Match: KAG7025505.1 (Protein indeterminate-domain 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 731.9 bits (1888), Expect = 3.8e-207
Identity = 397/522 (76.05%), Postives = 429/522 (82.18%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDP 60
           MFA+TMSNSTSLSEEA+VS           LN LLP        PQK KKKRSLPGNPDP
Sbjct: 1   MFATTMSNSTSLSEEATVS-----------LNSLLP--------PQKIKKKRSLPGNPDP 60

Query: 61  DAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV 120
           DAEVI LSP+TLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRN+KEVKKKAYV
Sbjct: 61  DAEVIALSPRTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNSKEVKKKAYV 120

Query: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE 180
           CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKC+KCSKVYAVQSDWKAHSKTCGTRE
Sbjct: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCEKCSKVYAVQSDWKAHSKTCGTRE 180

Query: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSST 240
           YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQ  +AA   AAA G       S+++
Sbjct: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQFVVAAEVEAAAEGGA---ATSTAS 240

Query: 241 TAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSN 300
            +AQSLF +G+ +QFS+SSLS P       Q +MSLNPWD+PQNPN N T QIIKPEDS+
Sbjct: 241 ASAQSLFPFGSFNQFSSSSLSRP------PQIYMSLNPWDNPQNPNPNQTHQIIKPEDSH 300

Query: 301 SHNFHQIPNLPLSNPS-SSNKNNPFGLIQDHHQ-------QKAGSSSSSSMITSPFRNLH 360
           SH+FHQIP LPL+  S S+  NNPFG+IQDHH+       QKAGSSSSSSMI SPFRNLH
Sbjct: 301 SHHFHQIPTLPLAGSSPSTATNNPFGIIQDHHRRHHPHHHQKAGSSSSSSMIASPFRNLH 360

Query: 361 VSVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPN 420
           VSV+P    A SAHLSATALLQKAATVGVGA KS  HQPESVGHMTHFN+P +PEF  P 
Sbjct: 361 VSVNP----AMSAHLSATALLQKAATVGVGAVKSSQHQPESVGHMTHFNMPDIPEFAAPT 420

Query: 421 HIDSLS-QLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLT 480
           H+DSL+ QLDPDYATWQKTDRLTRDFLGLTGDGN      GG GG G +QVSMSVKDMLT
Sbjct: 421 HMDSLTHQLDPDYATWQKTDRLTRDFLGLTGDGN------GGGGGSGAMQVSMSVKDMLT 480

Query: 481 YASGLEMFKPHNNNNNNNSNNN-----FGYAEPAANETWGDC 509
           YA GLE+FKPHNNNNNNN++NN     FG+AEPAANETW DC
Sbjct: 481 YAGGLEIFKPHNNNNNNNNSNNNNHSAFGFAEPAANETW-DC 483

BLAST of Lsi02G022560 vs. NCBI nr
Match: XP_023513763.1 (protein indeterminate-domain 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 729.6 bits (1882), Expect = 1.9e-206
Identity = 397/521 (76.20%), Postives = 428/521 (82.15%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDP 60
           MFA+TMSNSTSLSEEA+VS           LN LLP        PQK KKKRSLPGNPDP
Sbjct: 1   MFATTMSNSTSLSEEATVS-----------LNSLLP--------PQKIKKKRSLPGNPDP 60

Query: 61  DAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV 120
           DAEVI LSP+TLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRN+KEVKKKAYV
Sbjct: 61  DAEVIALSPRTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNSKEVKKKAYV 120

Query: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE 180
           CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKC+KCSKVYAVQSDWKAHSKTCGTRE
Sbjct: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCEKCSKVYAVQSDWKAHSKTCGTRE 180

Query: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSST 240
           YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQ   A AA   A+GA      S+++
Sbjct: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQ--FAIAAEVEAAGAEGGAATSTAS 240

Query: 241 TAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSN 300
            +AQSLF +G+ +QF +SSLS P       QT+MSLNPWD+PQNPN N T QIIKPEDS+
Sbjct: 241 ASAQSLFPFGSFNQFPSSSLSRP------PQTYMSLNPWDNPQNPNPNQTHQIIKPEDSH 300

Query: 301 SHNFHQIPNLPLSNPS-SSNKNNPFGLIQDHHQ-------QKAGSSSSSSMITSPFRNLH 360
           SH+FHQIP LPL+  S S+  NNPFG+IQDHH+       QKAGSSSSSSMI SPFRNLH
Sbjct: 301 SHHFHQIPTLPLAGSSPSTATNNPFGIIQDHHRRHHPHHHQKAGSSSSSSMIASPFRNLH 360

Query: 361 VSVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPN 420
           VSV+P    A SAHLSATALLQKAATVGVGA KS  HQPESVGHMTHFN+P +PEF  P 
Sbjct: 361 VSVNP----AMSAHLSATALLQKAATVGVGAVKSSQHQPESVGHMTHFNMPDIPEFAAPT 420

Query: 421 HIDSLS-QLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLT 480
           H+DSL+ QL PDYATWQKTDRLTRDFLGLTGDGN      GG GG G +QVSMSVKDMLT
Sbjct: 421 HMDSLTHQLGPDYATWQKTDRLTRDFLGLTGDGN------GGGGGSGAMQVSMSVKDMLT 480

Query: 481 YASGLEMFKPHNNNNNNNSNNN----FGYAEPAANETWGDC 509
           YA GLE+FKPHNNNNNNN+NNN    FG+AEPAANETW DC
Sbjct: 481 YAGGLEIFKPHNNNNNNNNNNNNHSAFGFAEPAANETW-DC 483

BLAST of Lsi02G022560 vs. NCBI nr
Match: XP_022959619.1 (protein indeterminate-domain 2-like [Cucurbita moschata])

HSP 1 Score: 728.4 bits (1879), Expect = 4.2e-206
Identity = 396/524 (75.57%), Postives = 427/524 (81.49%), Query Frame = 0

Query: 1   MFASTMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDP 60
           MFA+TMSNSTSLSEEA+VS           LN LLP        PQK KKKRSLPGNPDP
Sbjct: 1   MFATTMSNSTSLSEEATVS-----------LNSLLP--------PQKIKKKRSLPGNPDP 60

Query: 61  DAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYV 120
           DAEVI LSP+TLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRN+KEVKKKAYV
Sbjct: 61  DAEVIALSPRTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNSKEVKKKAYV 120

Query: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTRE 180
           CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKC+KCSKVYAVQSDWKAHSKTCGTRE
Sbjct: 121 CPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCEKCSKVYAVQSDWKAHSKTCGTRE 180

Query: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSST 240
           YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQ  +AA   A A G       S+++
Sbjct: 181 YRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQFVVAAEVEAVAEGGA---ATSTAS 240

Query: 241 TAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSN 300
            +AQSLF +GN +QF +SSLS P       QT+MSLNPWD+PQNPN N T QIIKPEDS+
Sbjct: 241 ASAQSLFPFGNLNQFPSSSLSRP------PQTYMSLNPWDNPQNPNPNQTHQIIKPEDSH 300

Query: 301 SHNFHQIPNLPLSNPS-SSNKNNPFGLIQDHHQ-------QKAGSSSSSSMITSPFRNLH 360
           SH+FHQIP LPL+  S S+  NNPFG+IQDHH+       QKAGSSSSSSMI SPFRNLH
Sbjct: 301 SHHFHQIPTLPLAGSSPSTATNNPFGIIQDHHRRHHPHHHQKAGSSSSSSMIASPFRNLH 360

Query: 361 VSVHPGSNAATSAHLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPN 420
           VSV+P    A SAHLSATALLQKAATVGVGA KS  HQPESVGHMTHFN+P +PEF  P 
Sbjct: 361 VSVNP----AMSAHLSATALLQKAATVGVGAVKSSQHQPESVGHMTHFNMPDIPEFAAPT 420

Query: 421 HIDSLS-QLDPDYATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLT 480
           H+DSL+ QL PDYATWQKTDRLTRDFLGLTGDGN      GG GG G +QVSMSVKDMLT
Sbjct: 421 HMDSLTHQLGPDYATWQKTDRLTRDFLGLTGDGN------GGGGGSGAMQVSMSVKDMLT 480

Query: 481 YASGLEMFKPHNNNNNNNSNNN-------FGYAEPAANETWGDC 509
           YA GLE+FKPHNNNNNNN+NN+       FG+AEPAANETW DC
Sbjct: 481 YAGGLEIFKPHNNNNNNNNNNSNNNNHSAFGFAEPAANETW-DC 485

BLAST of Lsi02G022560 vs. TAIR 10
Match: AT4G02670.1 (indeterminate(ID)-domain 12 )

HSP 1 Score: 350.1 bits (897), Expect = 2.9e-96
Identity = 226/480 (47.08%), Postives = 264/480 (55.00%), Query Frame = 0

Query: 10  TSLSEEASVSS---TTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDPDAEVIT 69
           +SLS EAS SS   T     +F+  + ++ S    H    KPKKKR LPGNPDPDAEVI 
Sbjct: 12  SSLSTEASASSGNNTLSTIQEFSGFHNVISSVC-THTETHKPKKKRGLPGNPDPDAEVIA 71

Query: 70  LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKE-VKKKAYVCPEPS 129
           LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQ+N KE  KKK YVCPE +
Sbjct: 72  LSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQKNTKEQQKKKVYVCPETN 131

Query: 130 CVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTREYRCDC 189
           C HHHPSRALGDLTGIKKH+CRKHGEKKWKC+KCSK YAVQSDWKAH+K CGTR+YRCDC
Sbjct: 132 CAHHHPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKFYAVQSDWKAHTKICGTRDYRCDC 191

Query: 190 GTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAAAVAAASGATDININSSSTTAAQS 249
           GTLFSRKD+FITHRAFCDALAEESARL +           ++S  T+ N N         
Sbjct: 192 GTLFSRKDTFITHRAFCDALAEESARLHS----------TSSSNLTNPNPN--------- 251

Query: 250 LFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKPEDSNSHNFH 309
              +  HH   N S SL     PL                        I+P         
Sbjct: 252 ---FQGHHFMFNKSSSLLFTSSPL-----------------------FIEP--------- 311

Query: 310 QIPNLPLSNPSSSNKNNPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVSVHPGSNAATSA 369
                                          S S++++ T P                +A
Sbjct: 312 -------------------------------SLSTAALSTPP----------------TA 371

Query: 370 HLSATALLQKAATVGVGAAKSGHHQPESVGHMTHFNIPSLPEFGPPNHIDSLSQLDPDY- 429
            LSATALLQKA ++       G  Q  S+GH  H ++ ++ EF   + +   S    +Y 
Sbjct: 372 ALSATALLQKATSLS-STTFGGGGQTRSIGH--HRHLTNVNEFLGVDRVMMTSASSSEYD 371

Query: 430 --------ATWQKTDRLTRDFLGLTGDGNNGVNGGGGSGGGGVVQVSMSVKDMLTYASGL 477
                   +TWQK DRLTRDFLGLTG G +               VS+   DML YA G+
Sbjct: 432 QLVVDGFTSTWQKADRLTRDFLGLTGHGGH---------------VSVRPGDMLEYAGGV 371

BLAST of Lsi02G022560 vs. TAIR 10
Match: AT1G03840.1 (C2H2 and C2HC zinc fingers superfamily protein )

HSP 1 Score: 328.9 bits (842), Expect = 7.0e-90
Identity = 201/389 (51.67%), Postives = 240/389 (61.70%), Query Frame = 0

Query: 40  DIHHSPQKP--KKKRSLPGNPDPDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHR 99
           D H S   P  KKKR+LPGNPDP+AEVI LSPKTL+ATNRF+CEIC KGFQRDQNLQLHR
Sbjct: 30  DQHESLNPPLVKKKRNLPGNPDPEAEVIALSPKTLMATNRFLCEICGKGFQRDQNLQLHR 89

Query: 100 RGHNLPWKLKQRNNKEVKKKAYVCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCD 159
           RGHNLPWKLKQR +KEV+K+ YVCPE SCVHHHP+RALGDLTGIKKH+CRKHGEKKWKC+
Sbjct: 90  RGHNLPWKLKQRTSKEVRKRVYVCPEKSCVHHHPTRALGDLTGIKKHFCRKHGEKKWKCE 149

Query: 160 KCSKVYAVQSDWKAHSKTCGTREYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQL 219
           KC+K YAVQSDWKAHSKTCGTREYRCDCGT+FSR+DSFITHRAFCDALAEE+ARL+A   
Sbjct: 150 KCAKRYAVQSDWKAHSKTCGTREYRCDCGTIFSRRDSFITHRAFCDALAEETARLNAAS- 209

Query: 220 EMAAAAVAAASGATDININSSSTTAAQSLFLYGNHHQFSNS---SLSLPLPP-----PPL 279
                           ++ S + TA  +L    N+H    +   S SLP PP     PP 
Sbjct: 210 ----------------HLKSFAATAGSNL----NYHYLMGTLIPSPSLPQPPSFPFGPPQ 269

Query: 280 SQTH------MSLNPWDHPQNPNSNHTLQIIKPEDSNSHNFHQIPNLPLSNPSSSNK--N 339
            Q H      ++ N +DH        TL +    + N H    I +     P S  +  N
Sbjct: 270 PQHHHHHQFPITTNNFDHQDVMKPASTLSLWSGGNINHHQQVTIEDRMAPQPHSPQEDYN 329

Query: 340 NPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVSVHPGSNAATS----------------- 387
             FG   +H +     ++S S+IT       V     +N ATS                 
Sbjct: 330 WVFGNANNHGEL---ITTSDSLITHDNNINIVQSKENANGATSLSVPSLFSSVDQITQDA 389

BLAST of Lsi02G022560 vs. TAIR 10
Match: AT1G03840.2 (C2H2 and C2HC zinc fingers superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 5.5e-87
Identity = 199/389 (51.16%), Postives = 238/389 (61.18%), Query Frame = 0

Query: 40  DIHHSPQKP--KKKRSLPGNPDPDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHR 99
           D H S   P  KKKR+LPGN  P+AEVI LSPKTL+ATNRF+CEIC KGFQRDQNLQLHR
Sbjct: 30  DQHESLNPPLVKKKRNLPGN--PEAEVIALSPKTLMATNRFLCEICGKGFQRDQNLQLHR 89

Query: 100 RGHNLPWKLKQRNNKEVKKKAYVCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCD 159
           RGHNLPWKLKQR +KEV+K+ YVCPE SCVHHHP+RALGDLTGIKKH+CRKHGEKKWKC+
Sbjct: 90  RGHNLPWKLKQRTSKEVRKRVYVCPEKSCVHHHPTRALGDLTGIKKHFCRKHGEKKWKCE 149

Query: 160 KCSKVYAVQSDWKAHSKTCGTREYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQL 219
           KC+K YAVQSDWKAHSKTCGTREYRCDCGT+FSR+DSFITHRAFCDALAEE+ARL+A   
Sbjct: 150 KCAKRYAVQSDWKAHSKTCGTREYRCDCGTIFSRRDSFITHRAFCDALAEETARLNAAS- 209

Query: 220 EMAAAAVAAASGATDININSSSTTAAQSLFLYGNHHQFSNS---SLSLPLPP-----PPL 279
                           ++ S + TA  +L    N+H    +   S SLP PP     PP 
Sbjct: 210 ----------------HLKSFAATAGSNL----NYHYLMGTLIPSPSLPQPPSFPFGPPQ 269

Query: 280 SQTH------MSLNPWDHPQNPNSNHTLQIIKPEDSNSHNFHQIPNLPLSNPSSSNK--N 339
            Q H      ++ N +DH        TL +    + N H    I +     P S  +  N
Sbjct: 270 PQHHHHHQFPITTNNFDHQDVMKPASTLSLWSGGNINHHQQVTIEDRMAPQPHSPQEDYN 329

Query: 340 NPFGLIQDHHQQKAGSSSSSSMITSPFRNLHVSVHPGSNAATS----------------- 387
             FG   +H +     ++S S+IT       V     +N ATS                 
Sbjct: 330 WVFGNANNHGEL---ITTSDSLITHDNNINIVQSKENANGATSLSVPSLFSSVDQITQDA 389

BLAST of Lsi02G022560 vs. TAIR 10
Match: AT1G55110.1 (indeterminate(ID)-domain 7 )

HSP 1 Score: 317.4 bits (812), Expect = 2.1e-86
Identity = 177/307 (57.65%), Postives = 213/307 (69.38%), Query Frame = 0

Query: 6   MSNSTSLS-EEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQ------KPKKKRSLPGNP 65
           MSN TS S ++ASVSS  R     + +N         HH  Q        K+KR+ PGNP
Sbjct: 21  MSNLTSASGDQASVSSGNRTETSGSNINQ--------HHQEQCFVPQSSLKRKRNQPGNP 80

Query: 66  DPDAEVITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKE-VKKK 125
           DP+AEV+ LSPKTL+ATNRF+CE+CNKGFQRDQNLQLH+RGHNLPWKLKQR+NK+ V+KK
Sbjct: 81  DPEAEVMALSPKTLMATNRFICEVCNKGFQRDQNLQLHKRGHNLPWKLKQRSNKDVVRKK 140

Query: 126 AYVCPEPSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCG 185
            YVCPEP CVHHHPSRALGDLTGIKKH+ RKHGEKKWKC+KCSK YAVQSDWKAH+KTCG
Sbjct: 141 VYVCPEPGCVHHHPSRALGDLTGIKKHFFRKHGEKKWKCEKCSKKYAVQSDWKAHAKTCG 200

Query: 186 TREYRCDCGTLFSRKDSFITHRAFCDALAEESARLSANQLEMAAA-AVAAASGATDININ 245
           T+EY+CDCGTLFSR+DSFITHRAFCDALAEESAR   N + + A+ +       T  NI 
Sbjct: 201 TKEYKCDCGTLFSRRDSFITHRAFCDALAEESARAMPNPIMIQASNSPHHHHHQTQQNIG 260

Query: 246 SSSTTAAQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDHPQNPNSNHTLQIIKP 304
            SS+          + +  SNS+L  P+        + ++ PW    NPN N     + P
Sbjct: 261 FSSS----------SQNIISNSNLHGPMKQEESQHHYQNIPPWLISSNPNPNGNNGNLFP 309

BLAST of Lsi02G022560 vs. TAIR 10
Match: AT2G02080.1 (indeterminate(ID)-domain 4 )

HSP 1 Score: 315.5 bits (807), Expect = 8.0e-86
Identity = 210/508 (41.34%), Postives = 280/508 (55.12%), Query Frame = 0

Query: 4   STMSNSTSLSEEASVSSTTRVHPDFTCLNPLLPSFSDIHHSPQKPKKKRSLPGNPDPDAE 63
           S + +S+S ++   ++S+     DF   +  +      + S   PKK+R+ PGNP+PDAE
Sbjct: 9   SVIPSSSSSAQPFFITSSGTGDNDFNRKDTFMSMIQQPNSSAPPPKKRRNQPGNPNPDAE 68

Query: 64  VITLSPKTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKEVKKKAYVCPE 123
           V+ LSPKTL+ATNRF+C++CNKGFQR+QNLQLHRRGHNLPWKLKQ++ KEVK+K Y+CPE
Sbjct: 69  VVALSPKTLMATNRFICDVCNKGFQREQNLQLHRRGHNLPWKLKQKSTKEVKRKVYLCPE 128

Query: 124 PSCVHHHPSRALGDLTGIKKHYCRKHGEKKWKCDKCSKVYAVQSDWKAHSKTCGTREYRC 183
           P+CVHH PSRALGDLTGIKKHY RKHGEKKWKC+KCSK YAVQSDWKAHSKTCGT+EYRC
Sbjct: 129 PTCVHHDPSRALGDLTGIKKHYYRKHGEKKWKCEKCSKRYAVQSDWKAHSKTCGTKEYRC 188

Query: 184 DCGTLFSRKDSFITHRAFCDALAEESAR-LSANQLEMAAAAVAAASGATDININSSSTTA 243
           DCGT+FSR+DS+ITHRAFCDAL +E+AR  + +   M AA+    SG     +   S  +
Sbjct: 189 DCGTIFSRRDSYITHRAFCDALIQETARNPTVSFTSMTAASSGVGSGGIYGRLGGGSALS 248

Query: 244 AQSLFLYGNHHQFSNSSLSLPLPPPPLSQTHMSLNPWDH-----PQNPNSNHTLQIIKPE 303
                   +HH   + +        PL   ++++   D+     PQ+ N N  +Q    +
Sbjct: 249 --------HHHLSDHPNFGF----NPLVGYNLNIASSDNRRDFIPQSSNPNFLIQSASSQ 308

Query: 304 D-------------SNSHNFHQIPNLPLSNPSSSNKNNPFGLIQDHHQQKAGSSSSSSMI 363
                          N H   Q   +   N  SS  NN F  +    +    S +S   +
Sbjct: 309 GMLNTTPNNNNQSFMNQHGLIQFDPVDNINLKSSGTNNSFFNLGFFQENTKNSETSLPSL 368

Query: 364 TSPFRNLHVSVHPGSNAATSAHLSATALLQKAATVGV-----------GAAKSGHHQPES 423
            S    +H   H   N    +++SATALLQKA  +G            G A S +     
Sbjct: 369 YSTDVLVH---HREENLNAGSNVSATALLQKATQMGSVTSNDPSALFRGLASSSNSSSVI 428

Query: 424 VGHMTHFNIPSLPEFGP-PNHIDSLSQLD--------------PDYATWQKTDRLTRDFL 467
             H     I      G     ++SL+ ++               D      +D+LT DFL
Sbjct: 429 ANHFGGGRIMENDNNGNLQGLMNSLAAVNGGGGSGGSIFDVQFGDNGNMSGSDKLTLDFL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O227594.1e-9547.08Protein indeterminate-domain 12 OS=Arabidopsis thaliana OX=3702 GN=IDD12 PE=2 SV... [more]
Q9ZWA69.8e-8951.67Zinc finger protein MAGPIE OS=Arabidopsis thaliana OX=3702 GN=MGP PE=1 SV=1[more]
Q8H1F53.0e-8557.65Protein indeterminate-domain 7 OS=Arabidopsis thaliana OX=3702 GN=IDD7 PE=2 SV=1[more]
Q8GYC11.1e-8441.34Protein indeterminate-domain 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SCQ61.2e-8346.37Zinc finger protein GAI-ASSOCIATED FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Match NameE-valueIdentityDescription
A0A0A0K7A51.2e-21785.18C2H2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G387730 P... [more]
A0A6J1H8L32.1e-20675.57protein indeterminate-domain 2-like OS=Cucurbita moschata OX=3662 GN=LOC11146064... [more]
A0A6J1GN295.1e-20575.53protein indeterminate-domain 9-like OS=Cucurbita moschata OX=3662 GN=LOC11145591... [more]
A0A6J1KRQ32.5e-20476.11protein indeterminate-domain 12-like OS=Cucurbita maxima OX=3661 GN=LOC111497661... [more]
A0A6J1JNM01.5e-19674.47protein indeterminate-domain 9-like OS=Cucurbita maxima OX=3661 GN=LOC111488512 ... [more]
Match NameE-valueIdentityDescription
XP_038897540.15.3e-25789.34zinc finger protein GAI-ASSOCIATED FACTOR 1-like [Benincasa hispida][more]
XP_011659290.17.7e-24084.34zinc finger protein BALDIBIS [Cucumis sativus] >KAE8646309.1 hypothetical protei... [more]
KAG7025505.13.8e-20776.05Protein indeterminate-domain 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023513763.11.9e-20676.20protein indeterminate-domain 2-like [Cucurbita pepo subsp. pepo][more]
XP_022959619.14.2e-20675.57protein indeterminate-domain 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G02670.12.9e-9647.08indeterminate(ID)-domain 12 [more]
AT1G03840.17.0e-9051.67C2H2 and C2HC zinc fingers superfamily protein [more]
AT1G03840.25.5e-8751.16C2H2 and C2HC zinc fingers superfamily protein [more]
AT1G55110.12.1e-8657.65indeterminate(ID)-domain 7 [more]
AT2G02080.18.0e-8641.34indeterminate(ID)-domain 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 154..174
e-value: 130.0
score: 3.4
coord: 78..100
e-value: 0.017
score: 24.3
coord: 119..149
e-value: 280.0
score: 0.5
IPR013087Zinc finger C2H2-typePFAMPF00096zf-C2H2coord: 78..100
e-value: 0.005
score: 17.2
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 80..100
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 78..100
score: 10.948853
NoneNo IPR availableGENE3D3.30.160.60Classic Zinc Fingercoord: 147..210
e-value: 3.3E-5
score: 25.8
NoneNo IPR availableGENE3D3.30.160.60Classic Zinc Fingercoord: 57..126
e-value: 2.8E-6
score: 29.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..63
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 483..508
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 483..498
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..343
NoneNo IPR availablePANTHERPTHR10593SERINE/THREONINE-PROTEIN KINASE RIOcoord: 8..490
NoneNo IPR availablePANTHERPTHR10593:SF136PROTEIN INDETERMINATE-DOMAIN 12coord: 8..490
IPR036236Zinc finger C2H2 superfamilySUPERFAMILY57667beta-beta-alpha zinc fingerscoord: 77..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G022560.1Lsi02G022560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding