Lsi02G021870 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G021870
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionmediator of RNA polymerase II transcription subunit 19a-like
Locationchr02: 28355766 .. 28359869 (-)
RNA-Seq ExpressionLsi02G021870
SyntenyLsi02G021870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACCCCAAATAAAATTGACATTTGAGCAAAAGGAAAGGGGAAGTTCCAAAGGAAGCCCTTGACCATATGTATTAAAAGTCCACTGTACGGTCCATTTGTAGATGTGGCAAAGGGTTATTTGCAGTATTATTCAGTGCCCCCTCTTCTCCTCTCTTCCCCGATCTCAATTGTCTCTCTTCTCCTCTCTCTCTCTCCTCTCTCCGCCGTTCCCGGCCGCCGGAGCTACTTCTTCACTCTTCTCACACCTCATTCTCCAACCCCTTTTCCTCTCAATTTCATCTCATTCTTCGATTCGATCCTCAATACCCTTTTTTCTCGATCGCTCTTTCAATCTCCTCTCATTTCCGGCGCCTCTTTACTTTTCTTCTACCCTTCGCCGCCGAGGTTCGTTTTCGTTTCTTTGTTTCTGGAATGGATTGCTTTAGAGTTGTTATTGGTTTTAAGCACTGCCCTTTTTTTCCCTTTCCCCTTCCAATCCCGGGGTTTTCAGTTCTGTGATTGATTGGTGTCTTTAGGGTTTCTTGCTGCCAGTCCTAGGTGTTTTGTACTTCGAGCTGAATTGGGTGTTTCGAATTTTGTTTTGGTTGCTGGGTTTTTGGGTTCTCGTTGAATTTAAAGAAGGAGACGTTTTTGGAGGTTGGGGTTGGGGTTCTTCTGAAATTGTGTTTCCCGCTTGTTTCATTCTTGTGTTTGAGTTCAGATGGATTTCTTGTTTTCATTTGGATAAGAGTGGTTTTAGCGTGGTCAATATAGGGATTGGGCTAGTTTCTGTTTTGTCTTCCCGATACAATTCTTTTCAAAATTTTAACCACTATTTCTCTTAGTTAATGGTTTTTGGATACATTTTATGACTCAAGTTTTCTTGTTTATAGGATTTGTTATGTGCATGATTTGCTAACAAACTTACAAAATGTTCTCGATTTGGACTGGAGGTTTCTGTATCTTAGATTTTTTTTTGAAGTGACCCAATTACTCCTCCAAGTTCTAATTAAATTTACCCTTTATTTATTTATTTTTATTTTATTTTTACTTTTTTAGTAAAACAAATGTGGGGTAGGGGAGTTTGAACCTCCAACCTCAAGGAAGGAGTACTGCTAAGCTATGCTTATGTTGACCTAGTTATATTTACTTTCTCCTTTAAACTAATCATTTAGTGATAACACATCATTTATGTTGTAACTCCTATATGTTTTTTGTTTTATGAAATGTCTAATGGAGTTAGGCATGCGTGATTCCCTTGTTAGACTCATACTTAAGAAATGCCTAATTTGGTTCACTTCTTTTCCTTTTTATATAAGCCATGGGTTTTAGCCTTTTCTGTTTAGATTGTTCCTTTGTAATTTTAATCTCTCGCGAACAAACAACAAGTTCATTGCATTTTTATCCACGAAATGATTTTTTTCCTTCCCATTATTAGAAAAGATAGAGAAAAATAGTATGTTTGTTGTCTTGGAATTATAATCTCACTACTCTTTGGGGTTTTATGATCGCAGTTATAATGTTACATTGTTCCAAGGAAGAGTGATACATGGAATCTGAAGATAAGAAATTTGGCCGAGGTAAATGTTGATATAGTCAATTTAGTCCCTTCTCCCTTGTGCACTGGCATGTTTAAAGAGTGAGACATAGCTTGGTATATATACATTGATGCTTTTACGTTTAGAAATCTTTAGATTCTGATGATACTATATTATCGTAAATGAGCATGCCTGTTTTGGGTGAAAACATGCCCTTGGTTTATCTATTATGTACCTGGGAACTTAATGATTAATATTTTATTTGGTATGTAAGGATCTCTTATGTGTATCTCTTGACTATTTATGTAGAAATTTATTTTCCTGTGTTTTCTCTTTTAGTTTCTATGAATGTGGTTAGTTGAGCTAGAGCATCTTATTCTAATACAATTTTTCCAAGCCATGCAGAGGGCTGTAGGATTATGGGTTGAAAGATGTAGTAAAACAAAGTTTTGCTTGATCTGGAATTGTGGTAGGAAATTTATAGTTCAAGTTTAGAGCTGAATTAAACCACTTGAGCGTTTAGAAGAGGTTTTACCTTTAGAAGAGGCTTAAGGGTTTACCTATGTTTTTTGATACTTTTTTCTTCCAATGTACAAGAATTATTTATTTATTTAATGTAATGCAAATCAGGGGATTTTGAAATAGCAATTTTGGTGTACTTAAAAATTTTGATATTTTGAATGGGATACTATTATATCTTTGATACTTTGGCTACCACTTTACTGATGGATTTCACAGAGATTGCATAATGAAATTTAGCCCATACAAATCATTTGACTCTTTTTGTTGCAATAATATAGGAGCAACTTTAATTTATCAGATGTCCTCAAAGGGACATATAGGAAGTGAGCTATGATGACTTGACATTTCTTTATTTAGTCTCTCTTTGGCTTGAGCAATGAAAATTGGCCCACGTCTTACTTAGGTCTTCCTATGATTGGGAGCTCAAAGTGTGACCTTCTTACTTCAATTATTGAAAGGACTTTCTGTAATTGAGAACTTGAAGTGTCTTGAATTTTGGATTCCAAGTATTGAAAAAACATTGCGAGCTTTAGTATTGGAGGAGATTGGTCCTCTCATTCATGCCTTCTTGCCAAATATTCTGTGAGACAGAGTGTTCAGTGCAGGAAGTGAATGAGTTCTGTACCCCACTGCAAGCTAAACTCTAAACAGTTTTCCCCTAAAATTACATATGGTCTATGCCATGCCTTGAAAATTGCATTTTAAGATCTTCAGGATTTCTGAAGTGAAAGAATTATTCACGGTTTTCTATTTACTTTAAAGCAATTTGGTCTTTCTTTGCAATGGTTCAATGTTACTTTATAACAACAACAACAATATTCCAAACCAAAAACAACAACAACAATAATAAAAATAATAATATTTTTTTTTCTCTTTACAATTCTTTCTTCACTTTTGTTTATTTTTTTGAATATTATTACTGTAGCTCCTTTTGTCTTTCCATGTTGTACATTTTAATTCTTTAATTGATTTTTTCTAATCAGTTAGCTTTTTCTTTCTTTTGTTAGTATATGTCTTTATTGCTTTTGTTGCAATGTAATTGAAAGGAACATGGAAGTACAAGATATAGCTGTTTTCTGATCCATCTTGTTCATTTTCTATCTGTATATATTATCTTGTCACCTGAATTTTTGGTATATTTTTATTTGTACCTTTAGGACCTAGGGAACTGACTGGTGCTGTGGATCTTATAAGTCACTACAAGCTGTTACCTCACCATGATTTTTTCTGCAAGAAGTCACTTCCACTGTCAATTTCGGACACACATTATCTTCATAACGTGGTTGGAGACACAGAAATTAGAAAAGGAGAAGGGATGCAGTTGAATCAACTCATACAGACTTCTTACCCTAGAGAGACTAATGCCCGCATACAGCCATTTGACCAAGATACTCTCATAGAAGCCTTCCAGCTGAGGGAAACTGGTCCTGTTGATCTGCCTTCTGTAAGAAATCTATTATTAAAATTGTTAAAATGAGCCACTTTTTTCAGCTTTCTTCCATCAGCTGGAATTTAGATGTCACCAAGGCAACGGGTCTGATTATCAGCATTTCTCATCCTGATTCTTTTCGCTGCAAGTGATGGTCCATTTCTTTTTCTTTGGTTAGGCCGAGAAGGGTGTTCCTACTATTCCAGGGAAGTCAAAAAGCGAGTCCAAAGATAAAGACAGAAAGCATAAGAAGCACAAAGATAGGGATAAGGACAAGGATAGAGAGCATAAGAAGCACAAGCATCGACATAAAGACCGAAGTAAGGATAAAGACAAGGACAAGAAGAAAGACAAAAGTGCCCATCAAGATTCTGGTGCTGATCATTCTAAGAAGCATCATGAAAAGGTTGGAAAACCTTTACTCTTTTCCAGCTCCTTTGTAAATACCAACTACCAAGATTTGATAACAATGGGATGGTTCAGGAGTAGATTGTCATTGACATTAACATTATGGATTGTTTAGTGTATCCCAATCCCTAGTTTGTTGTTTCAGTTACAAGATTATCACTTCTTGAAGTGTATCCCTTTCACTGAATTTTGTTCATTTGACCTTAGATTATCAAACCCTTAG

mRNA sequence

AAAAAAACCCCAAATAAAATTGACATTTGAGCAAAAGGAAAGGGGAAGTTCCAAAGGAAGCCCTTGACCATATGTATTAAAAGTCCACTGTACGGTCCATTTGTAGATGTGGCAAAGGGTTATTTGCAGTATTATTCAGTGCCCCCTCTTCTCCTCTCTTCCCCGATCTCAATTGTCTCTCTTCTCCTCTCTCTCTCTCCTCTCTCCGCCGTTCCCGGCCGCCGGAGCTACTTCTTCACTCTTCTCACACCTCATTCTCCAACCCCTTTTCCTCTCAATTTCATCTCATTCTTCGATTCGATCCTCAATACCCTTTTTTCTCGATCGCTCTTTCAATCTCCTCTCATTTCCGGCGCCTCTTTACTTTTCTTCTACCCTTCGCCGCCGAGTTATAATGTTACATTGTTCCAAGGAAGAGTGATACATGGAATCTGAAGATAAGAAATTTGGCCGAGGACCTAGGGAACTGACTGGTGCTGTGGATCTTATAAGTCACTACAAGCTGTTACCTCACCATGATTTTTTCTGCAAGAAGTCACTTCCACTGTCAATTTCGGACACACATTATCTTCATAACGTGGTTGGAGACACAGAAATTAGAAAAGGAGAAGGGATGCAGTTGAATCAACTCATACAGACTTCTTACCCTAGAGAGACTAATGCCCGCATACAGCCATTTGACCAAGATACTCTCATAGAAGCCTTCCAGCTGAGGGAAACTGGTCCTGTTGATCTGCCTTCTGCCGAGAAGGGTGTTCCTACTATTCCAGGGAAGTCAAAAAGCGAGTCCAAAGATAAAGACAGAAAGCATAAGAAGCACAAAGATAGGGATAAGGACAAGGATAGAGAGCATAAGAAGCACAAGCATCGACATAAAGACCGAAGTAAGGATAAAGACAAGGACAAGAAGAAAGACAAAAGTGCCCATCAAGATTCTGGTGCTGATCATTCTAAGAAGCATCATGAAAAGGTTGGAAAACCTTTACTCTTTTCCAGCTCCTTTTGTATCCCAATCCCTAGTTTGTTGTTTCAGTTACAAGATTATCACTTCTTGAAGTGTATCCCTTTCACTGAATTTTGTTCATTTGACCTTAGATTATCAAACCCTTAG

Coding sequence (CDS)

ATGGAATCTGAAGATAAGAAATTTGGCCGAGGACCTAGGGAACTGACTGGTGCTGTGGATCTTATAAGTCACTACAAGCTGTTACCTCACCATGATTTTTTCTGCAAGAAGTCACTTCCACTGTCAATTTCGGACACACATTATCTTCATAACGTGGTTGGAGACACAGAAATTAGAAAAGGAGAAGGGATGCAGTTGAATCAACTCATACAGACTTCTTACCCTAGAGAGACTAATGCCCGCATACAGCCATTTGACCAAGATACTCTCATAGAAGCCTTCCAGCTGAGGGAAACTGGTCCTGTTGATCTGCCTTCTGCCGAGAAGGGTGTTCCTACTATTCCAGGGAAGTCAAAAAGCGAGTCCAAAGATAAAGACAGAAAGCATAAGAAGCACAAAGATAGGGATAAGGACAAGGATAGAGAGCATAAGAAGCACAAGCATCGACATAAAGACCGAAGTAAGGATAAAGACAAGGACAAGAAGAAAGACAAAAGTGCCCATCAAGATTCTGGTGCTGATCATTCTAAGAAGCATCATGAAAAGGTTGGAAAACCTTTACTCTTTTCCAGCTCCTTTTGTATCCCAATCCCTAGTTTGTTGTTTCAGTTACAAGATTATCACTTCTTGAAGTGTATCCCTTTCACTGAATTTTGTTCATTTGACCTTAGATTATCAAACCCTTAG

Protein sequence

MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRKGEGMQLNQLIQTSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSKSESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKHHEKVGKPLLFSSSFCIPIPSLLFQLQDYHFLKCIPFTEFCSFDLRLSNP
Homology
BLAST of Lsi02G021870 vs. ExPASy Swiss-Prot
Match: Q9FMP0 (Mediator of RNA polymerase II transcription subunit 19a OS=Arabidopsis thaliana OX=3702 GN=MED19A PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.1e-67
Identity = 144/190 (75.79%), Postives = 160/190 (84.21%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           ME E  KFG GPREL GA DLIS +KL+ HH+FFCKKSLP+S+SD+HYLHNVVGDTEIRK
Sbjct: 1   MEPERLKFG-GPRELCGAADLISQFKLVQHHEFFCKKSLPVSLSDSHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQT-SYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQL+QLI++ S  RETN RIQPFD D L E+FQL +  PV+LP AEKG PTIP KSK
Sbjct: 61  GEGMQLDQLIESISQSRETNIRIQPFDIDELQESFQLNDMTPVELPPAEKGAPTIPSKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDK----KKDKSAHQDSGADH 180
           SESKD+DRKHKKHKDRDKDKDREHKKHKH+HKDRSKDKDKDK    KKDK+ H DSG DH
Sbjct: 121 SESKDRDRKHKKHKDRDKDKDREHKKHKHKHKDRSKDKDKDKDRDRKKDKNGHHDSG-DH 180

Query: 181 SKKHHEKVGK 186
           SKKHH+K  K
Sbjct: 181 SKKHHDKKRK 188

BLAST of Lsi02G021870 vs. ExPASy Swiss-Prot
Match: Q6NQD9 (Probable mediator of RNA polymerase II transcription subunit 19b OS=Arabidopsis thaliana OX=3702 GN=MED19B PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 7.6e-57
Identity = 125/182 (68.68%), Postives = 146/182 (80.22%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESE  KFG GPREL GA+DLI+ YKLLPHH+FFCK+SLP S+SD HYLHN+VGDTEIRK
Sbjct: 1   MESESVKFG-GPRELGGALDLITQYKLLPHHEFFCKRSLPESLSDAHYLHNLVGDTEIRK 60

Query: 61  GEGMQLNQLIQTS--YPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKS 120
           GEGMQL+QLI  +    R+TNARIQPF  D L EAF+L +T PV+LP AEKG PT   KS
Sbjct: 61  GEGMQLDQLIPNASLSSRDTNARIQPFVLDELKEAFELNDTAPVELPPAEKGAPTTVSKS 120

Query: 121 KSESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKK 180
           KSESKDKDRKH+KHKD+ K+KDREHKKHKH+HKDR KDKDKDK +DK   +    D  +K
Sbjct: 121 KSESKDKDRKHRKHKDK-KEKDREHKKHKHKHKDRIKDKDKDKDRDKKKEKSGHHDKKRK 180

BLAST of Lsi02G021870 vs. ExPASy TrEMBL
Match: A0A1S4DWJ4 (mediator of RNA polymerase II transcription subunit 19a-like OS=Cucumis melo OX=3656 GN=LOC103489709 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.2e-90
Identity = 180/186 (96.77%), Postives = 181/186 (97.31%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLPSAEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDILIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKKKDKS HQDSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKKDKSGHQDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. ExPASy TrEMBL
Match: A0A0A0K716 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G374560 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 9.2e-90
Identity = 179/186 (96.24%), Postives = 181/186 (97.31%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLPSAEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDILIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKKK+KS HQDSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKKEKSGHQDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. ExPASy TrEMBL
Match: A0A6J1H5Q1 (mediator of RNA polymerase II transcription subunit 19a-like OS=Cucurbita moschata OX=3662 GN=LOC111460791 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.3e-88
Identity = 177/186 (95.16%), Postives = 180/186 (96.77%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESEDKKFG+GPRELTGAVDLISHYKLLPHH+FFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MESEDKKFGQGPRELTGAVDLISHYKLLPHHEFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQT-SYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ  SYPRETNARIQPFDQD LIEAFQLRETGPV+LP AEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNPSYPRETNARIQPFDQDILIEAFQLRETGPVELPPAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. ExPASy TrEMBL
Match: A0A6J1KVN1 (mediator of RNA polymerase II transcription subunit 19a-like OS=Cucurbita maxima OX=3661 GN=LOC111498006 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.9e-88
Identity = 175/186 (94.09%), Postives = 180/186 (96.77%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESEDKKFG+GPRELTGAVDLISHYKLLPHH+FFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MESEDKKFGQGPRELTGAVDLISHYKLLPHHEFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQT-SYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ+ SYPRETNARIQPFDQD LIEAFQLRETGPV+LP AEKGVPT+PGKSK
Sbjct: 61  GEGMQLNQLIQSPSYPRETNARIQPFDQDILIEAFQLRETGPVELPPAEKGVPTVPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
            ESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH
Sbjct: 121 GESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. ExPASy TrEMBL
Match: A0A6J1CP39 (mediator of RNA polymerase II transcription subunit 19a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013422 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 8.6e-88
Identity = 175/186 (94.09%), Postives = 180/186 (96.77%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESEDKKFGRGPRELTGAVDLISHYKLLPHH+FFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHEFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLP AEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDILIEAFQLRETGPVDLPPAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDK+RKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKK+DKSAH +SGADHSKKH
Sbjct: 121 SESKDKERKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKRDKSAHHESGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. NCBI nr
Match: XP_016900359.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 19a-like [Cucumis melo])

HSP 1 Score: 341.3 bits (874), Expect = 6.6e-90
Identity = 180/186 (96.77%), Postives = 181/186 (97.31%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLPSAEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDILIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKKKDKS HQDSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKKDKSGHQDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. NCBI nr
Match: XP_038898853.1 (mediator of RNA polymerase II transcription subunit 19a-like isoform X3 [Benincasa hispida])

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-89
Identity = 179/186 (96.24%), Postives = 181/186 (97.31%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           ME+EDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MEAEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLPSAEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDVLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKKKDKSAH DSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKKDKSAHHDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. NCBI nr
Match: XP_038898851.1 (mediator of RNA polymerase II transcription subunit 19a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-89
Identity = 179/186 (96.24%), Postives = 181/186 (97.31%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           ME+EDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MEAEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLPSAEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDVLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKKKDKSAH DSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKKDKSAHHDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. NCBI nr
Match: XP_038898852.1 (mediator of RNA polymerase II transcription subunit 19a-like isoform X2 [Benincasa hispida])

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-89
Identity = 179/186 (96.24%), Postives = 181/186 (97.31%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           ME+EDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MEAEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLPSAEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDVLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKKKDKSAH DSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKKDKSAHHDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. NCBI nr
Match: KGN45175.2 (hypothetical protein Csa_016228 [Cucumis sativus])

HSP 1 Score: 339.7 bits (870), Expect = 1.9e-89
Identity = 179/186 (96.24%), Postives = 181/186 (97.31%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK
Sbjct: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQ-TSYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQLNQLIQ TSYPRETNARIQPFDQD LIEAFQLRETGPVDLPSAEKGVPTIPGKSK
Sbjct: 61  GEGMQLNQLIQNTSYPRETNARIQPFDQDILIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKKH 180
           SESKDKDRKHKKHKDRDK+KDREHKKHKHRHKDRSKDKDKDKKK+KS HQDSGADHSKKH
Sbjct: 121 SESKDKDRKHKKHKDRDKEKDREHKKHKHRHKDRSKDKDKDKKKEKSGHQDSGADHSKKH 180

Query: 181 HEKVGK 186
           HEK  K
Sbjct: 181 HEKKRK 186

BLAST of Lsi02G021870 vs. TAIR 10
Match: AT5G12230.1 (MED19A; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G19480.2); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 256.9 bits (655), Expect = 1.5e-68
Identity = 144/190 (75.79%), Postives = 160/190 (84.21%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           ME E  KFG GPREL GA DLIS +KL+ HH+FFCKKSLP+S+SD+HYLHNVVGDTEIRK
Sbjct: 1   MEPERLKFG-GPRELCGAADLISQFKLVQHHEFFCKKSLPVSLSDSHYLHNVVGDTEIRK 60

Query: 61  GEGMQLNQLIQT-SYPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKSK 120
           GEGMQL+QLI++ S  RETN RIQPFD D L E+FQL +  PV+LP AEKG PTIP KSK
Sbjct: 61  GEGMQLDQLIESISQSRETNIRIQPFDIDELQESFQLNDMTPVELPPAEKGAPTIPSKSK 120

Query: 121 SESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDK----KKDKSAHQDSGADH 180
           SESKD+DRKHKKHKDRDKDKDREHKKHKH+HKDRSKDKDKDK    KKDK+ H DSG DH
Sbjct: 121 SESKDRDRKHKKHKDRDKDKDREHKKHKHKHKDRSKDKDKDKDRDRKKDKNGHHDSG-DH 180

Query: 181 SKKHHEKVGK 186
           SKKHH+K  K
Sbjct: 181 SKKHHDKKRK 188

BLAST of Lsi02G021870 vs. TAIR 10
Match: AT5G19480.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12230.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 221.9 bits (564), Expect = 5.4e-58
Identity = 125/182 (68.68%), Postives = 146/182 (80.22%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESE  KFG GPREL GA+DLI+ YKLLPHH+FFCK+SLP S+SD HYLHN+VGDTEIRK
Sbjct: 1   MESESVKFG-GPRELGGALDLITQYKLLPHHEFFCKRSLPESLSDAHYLHNLVGDTEIRK 60

Query: 61  GEGMQLNQLIQTS--YPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKS 120
           GEGMQL+QLI  +    R+TNARIQPF  D L EAF+L +T PV+LP AEKG PT   KS
Sbjct: 61  GEGMQLDQLIPNASLSSRDTNARIQPFVLDELKEAFELNDTAPVELPPAEKGAPTTVSKS 120

Query: 121 KSESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKK 180
           KSESKDKDRKH+KHKD+ K+KDREHKKHKH+HKDR KDKDKDK +DK   +    D  +K
Sbjct: 121 KSESKDKDRKHRKHKDK-KEKDREHKKHKHKHKDRIKDKDKDKDRDKKKEKSGHHDKKRK 180

BLAST of Lsi02G021870 vs. TAIR 10
Match: AT5G19480.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12230.1); Has 15754 Blast hits to 7644 proteins in 441 species: Archae - 6; Bacteria - 451; Metazoa - 7628; Fungi - 1448; Plants - 1196; Viruses - 38; Other Eukaryotes - 4987 (source: NCBI BLink). )

HSP 1 Score: 221.9 bits (564), Expect = 5.4e-58
Identity = 125/182 (68.68%), Postives = 146/182 (80.22%), Query Frame = 0

Query: 1   MESEDKKFGRGPRELTGAVDLISHYKLLPHHDFFCKKSLPLSISDTHYLHNVVGDTEIRK 60
           MESE  KFG GPREL GA+DLI+ YKLLPHH+FFCK+SLP S+SD HYLHN+VGDTEIRK
Sbjct: 1   MESESVKFG-GPRELGGALDLITQYKLLPHHEFFCKRSLPESLSDAHYLHNLVGDTEIRK 60

Query: 61  GEGMQLNQLIQTS--YPRETNARIQPFDQDTLIEAFQLRETGPVDLPSAEKGVPTIPGKS 120
           GEGMQL+QLI  +    R+TNARIQPF  D L EAF+L +T PV+LP AEKG PT   KS
Sbjct: 61  GEGMQLDQLIPNASLSSRDTNARIQPFVLDELKEAFELNDTAPVELPPAEKGAPTTVSKS 120

Query: 121 KSESKDKDRKHKKHKDRDKDKDREHKKHKHRHKDRSKDKDKDKKKDKSAHQDSGADHSKK 180
           KSESKDKDRKH+KHKD+ K+KDREHKKHKH+HKDR KDKDKDK +DK   +    D  +K
Sbjct: 121 KSESKDKDRKHRKHKDK-KEKDREHKKHKHKHKDRIKDKDKDKDRDKKKEKSGHHDKKRK 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMP02.1e-6775.79Mediator of RNA polymerase II transcription subunit 19a OS=Arabidopsis thaliana ... [more]
Q6NQD97.6e-5768.68Probable mediator of RNA polymerase II transcription subunit 19b OS=Arabidopsis ... [more]
Match NameE-valueIdentityDescription
A0A1S4DWJ43.2e-9096.77mediator of RNA polymerase II transcription subunit 19a-like OS=Cucumis melo OX=... [more]
A0A0A0K7169.2e-9096.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G374560 PE=4 SV=1[more]
A0A6J1H5Q11.3e-8895.16mediator of RNA polymerase II transcription subunit 19a-like OS=Cucurbita moscha... [more]
A0A6J1KVN13.9e-8894.09mediator of RNA polymerase II transcription subunit 19a-like OS=Cucurbita maxima... [more]
A0A6J1CP398.6e-8894.09mediator of RNA polymerase II transcription subunit 19a-like isoform X2 OS=Momor... [more]
Match NameE-valueIdentityDescription
XP_016900359.16.6e-9096.77PREDICTED: mediator of RNA polymerase II transcription subunit 19a-like [Cucumis... [more]
XP_038898853.11.5e-8996.24mediator of RNA polymerase II transcription subunit 19a-like isoform X3 [Beninca... [more]
XP_038898851.11.5e-8996.24mediator of RNA polymerase II transcription subunit 19a-like isoform X1 [Beninca... [more]
XP_038898852.11.5e-8996.24mediator of RNA polymerase II transcription subunit 19a-like isoform X2 [Beninca... [more]
KGN45175.21.9e-8996.24hypothetical protein Csa_016228 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G12230.11.5e-6875.79MED19A; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G19... [more]
AT5G19480.15.4e-5868.68unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G19480.25.4e-5868.68unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019403Mediator complex, subunit Med19, metazoaPFAMPF10278Med19coord: 12..179
e-value: 1.4E-8
score: 34.8
IPR019403Mediator complex, subunit Med19, metazoaPANTHERPTHR22536LUNG CANCER METASTASIS-RELATED LCMR1 PROTEINcoord: 1..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 157..180
NoneNo IPR availablePANTHERPTHR22536:SF4MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19A-LIKE ISOFORM X1coord: 1..182

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G021870.1Lsi02G021870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006777 Mo-molybdopterin cofactor biosynthetic process
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0016592 mediator complex
cellular_component GO:0019008 molybdopterin synthase complex
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0061798 GTP 3',8'-cyclase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003712 transcription coregulator activity