Lsi02G021840 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G021840
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionacyl-coenzyme A thioesterase 9, mitochondrial
Locationchr02: 28330927 .. 28335140 (+)
RNA-Seq ExpressionLsi02G021840
SyntenyLsi02G021840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTCATGTAAATGTGGTGATTGAAACAGTTGGATGTTTGTTTATAAAGCAGAGGCAATGTGGAAGATCAAAAGCAAAGTCCAACACTCTGTTCAAGTTCAAGCTTTGAACTCTGTTTTTTCCCTCAAATCTTGGAGTTCAACCCCAAAACCCAAATTCAATTCCCAGTTCCAACGTTCATTTTCCATGGATTCCAATTCTAATTCCTCTCCTTCGAGGACTATTTCAGTATTCTCTACCATTCCTCTGCCGCCGAGTTCCGGCGCCGTCGATGGTTCCGGGAGTTCCACTTCGGCTCGGAAACCGATCAGTCTCTGGCCGGGCATGCACCATTCGCCGGTGACCACCGCCCTTTGGGAAGCTAGGTCGAGTATCTTTGAGAGGTTGCTTGATCCTCCTAAAGATGCGCCGCCGCAGAGTGAATTGCTTACCAAAACGCCTTCTCAGAGTCGGACTATTGTAATTTATAATTTCTCTTCTGATTTTATTCTGAGAGAGCAGTATCGAGATCCGTGGAATGAGGTCAGAATCGGAAAGTTGCTTGAAGATCTTGATGCCTTGGCCGGTACCATCTCCGTTAGAGTATGACACTTTCTTCGTTGCTCCTTTTGCTTTTCCATGGGGTTTTTTTTTCCCTCTTTCTGAATGCTCTGCGTCTGCTGGAATTGATAATGGATATATGATGATTCTTTGGACTGAATGGCTTATTGAATGCAATTTCCTAAACATACTATTCGCCCTAATTTTGTTGTTTTTCCCCCTTTTTGTAATCTTCATCTCTAGGACCTTTTGTTAGGAATTTGAAGTTTCGGAGATGAAACTGTCTTTTGGCTTGAAATTTTCGACTCTGTGAAATGCTTTCATCCGTTATGATTTTTTCCCCCCACATTTTACAGAAATTAAGAGGGAGTTCAAATTCATTACTGTTTAGATGAGGTAGTGATCTTTGAATCATGATGAGGTAGGGATATTTCAATCATTAAATTATAAAGAGGGAAAGATACTTTAGCTGCTAATCATATATGCCCATCATTTTCAGCAACTTAGTGGTTTGAATTTTATGTATATATAAATAAGAGCTTCAAATTGAAGTTAGGTGGAACCTTTTAAGCGGCTTTGACTGAGATGCATGAGTGAGCAAGAAAATGAATTGGTATTTGGTATTTGGTATTTGGTATTTGGTATTTGGTATTTGGTAAAGGGTTTCTGCACTATGAATCTATCAAATTATTTACCGTCCCAGGAATTTATTTGTCCTGGTTTCCTCTTTGACAGCACTGTTCTGATGATGATAGCACAACAAGACCACTTTTGCTAGTGACTGCCTCTGTTGACAGAATTGTTCTAAAGAAGCCAATTAGTGTTGATGCTGATTTGAAAATAGTTGGTTCAGTCATATGGGTTGGGCGATCCTCAATTGAGGTTCAGTTAGACATGATTCAGTCCACAGAAGGTATGAATTAAGAAATATGGTGAATCATGACTATTCGTTCTGTTTGAAAAATTATTGTCCTATTAACCTCTCTATCACTCTCTCTCTTTTAAGAAATGAATTTCAGATTTTCACGTGGTTCATTGGGGCGACTCTGCCTTAATTTATCCTCCTCTCTTAGAATAACATACTATAAACTTTGAGAGTATGATAGACAGTGAAACAAAAGAAAAGTGGACAATCTTTAGTTATTGTTATATCAGCATTGCAGTACAGTAATTTAGTTGTCTTTCCAGCTTGCTAACAGTGACGTTTAGTTGGAGTGCTGCTAATTGACATGAGAACATTCCAGAGTGAGAACTCCAAACAGTTAGAATTTTGATTAGAAATGAAGTCTTCTCGCTTCATGGTAATGTAGAGCTCACTACCACTGTGATCGCATTCTCTTCAAAGGGCCTTTTCGATCTCATCTTCCAGTCATTAGGAATTGGACTCTGGCCCAAACTTTGAAGCATGAATCTTGGGCTAGTTTCTACGATATACAAATTTTTTTTAATTAGTTATAACTTCTATTCTATCTCAATGACTTGATCTTTCTGAAAGGTGTCTACACTACTGATTATCATAGTTTCCTTACTCATTATCTCTATCAAGGGATGTTTATCATCTACTGATCTACTAACAGTTCTGAATGTCCACTTCCAAAGCAAAACCCAAAGAATAAGAGATGATCTCACCATACTCATTCAAAATGGAGACTTTTTACCGTCCTCCAAAAAGCAACAGAAGATTCTTACAAGTTACATCACCTTGTTAGTTTTTAAGAGTCTCCTCTTGCTTACGAAAATTTGTGCCGAACCTCAAATAGTAATACTGAGGTTAAAATTTTATATTTTGGAGTTTAGAGAGATGTTTTGTTCAACATCTTTTTGCTTGGTGTCTGGTGCCAACTACCTATTAGCTGCTAAATTGATAGGAAATGATGCATTTTCATCATCAATCTTGCCTTTTTGGTTCTTCTGCAGATTGCTCAAACCAGTCAGACTCGGTTGCTCTAACAGCAAACTTCATATTTGTGGCACGCGACTCTCAGACTGGGAAAGCTGCTTTGGTTAACAGACTTTCGCCAGAAACTGAGGAAGAAAAATTACTTTTTGAGGGAGCAGAAGCAAGGAGTAAATTAAGGAAAAGGAAGAATAGAGGTGATTTGTGGGGTTTGGATAATGGGTACATAAAAAGAATTGATGCTTTGCTAGCAGAGGGAAGAATCTTCTGTGACATGCCAGCCCTGGCAGACAGAGACAGCATTCTTCTCAGGGATACTCGCCTTGAAAACTCTTTGATTTGTCAACCACAACAAAGAAACATACATGGTCGGATTTTCGGAGGGTTTCTTATGCAACGAGCATTTGAGTTGGCTTTCTCAACAGCATATGTGTTTGCCGGAATGGTGCCTTACTTTTTTGAAGTTGATCATGTTGATTTCTTAAAACCTGTGAGTGCACATCTCTTTTACACTTCTACTTATCTTTCTCTTCGATAGAAAGGAAAAATATCTCAATAGTAAAAGTGTTGGTTGTTTCGATGTGCAGGTGGATGTTGGTGACTTTCTACGTTTCACATCATGTGTTCTTTACACAGAAGTCGACAATCCGGATCAGCCATTGATCAACATTGAAGTTGTTGCCCATGTTACTAGGCCCGAGATCAGGTCTAGCGAGGTAACGATCCTGTATATGCTAATACCATCCCAAAAAATCTATGGTTTAAATGCAGTTTGTCTTTTTACTTTTATATTTATATTGTTATGTTTTTAACATTTGAAATGTTTTGAAATGTTTTGTTTTGGTCTCTAAACATATGTTAAAATGGTCCTTGTCATTACATTTTACATAGCATCCAACTCTAATTGAGACCATGTATTTCCAAACAGGATACCAAACTGATAACATATAGCTCAAAACATAAATATATGTGGTGCCTTGATTGATTGTTGTGAAAATGAGGTACTATAGTAGATTGTTTGAAAATATAAGCTTAAATCTGAATTTTGAACAACGTGTAAAAGTTTAAAAATAGGATTTAAACCTATCTCTACATCTGTCTCATTGACTCATAAAAAACATCAGTCCTATAGGGATGTATCAAATGGCAAAATTGTTTTGTCATAAAAACTGTAAATTTATGTAAAAATGAGGTAAAATGAGAGTTTCTAATGTTAGTTTAGTTACTCATTGACAATCTCTAAAACCTTGTAAACTTACTAATTGGTTGTGATTTACTAATCCAATGCAGGTGTCAAATACATTCTATTTCACTTTCACTGTGCGTCCGGAGGCAAAAGCTAAGGGAAACGGATTCAGACTTCGCAACGTCGTTCCAGCAACAGAAGAAGAAGCTCGTCGTATCATTGAGCGGATGGATGCCGAAAAAGATGCTAAGGGACTTGAGCAAGCAATCTGAATCAGGTCAGGCTTAGGCAGCCAACACAGTTTGTCTTGGTTTAAGAAAATCTTGTCGCATCCTTATTTTGAAATTCGGCTAAGTTATAGAAAACTGATCTTGTAGAATATTTGTGAAGGAAATCAATCTTATTCAATTACTGTTGGAGGGAGAAGAGCAATCAAATTATGTAATGGAGTATTGATTATTTAAGCCCTATTTGGTAATCTTTAGTTTTTAGTTTTTTAAAATTAAGCATATAAACATTCATTTCAACAAAAAAGTGTTGAAATAAAAATTGCTTTGTTATCTACTTTTTATCAATGTTTTCAAAACCAAG

mRNA sequence

TGTTCATGTAAATGTGGTGATTGAAACAGTTGGATGTTTGTTTATAAAGCAGAGGCAATGTGGAAGATCAAAAGCAAAGTCCAACACTCTGTTCAAGTTCAAGCTTTGAACTCTGTTTTTTCCCTCAAATCTTGGAGTTCAACCCCAAAACCCAAATTCAATTCCCAGTTCCAACGTTCATTTTCCATGGATTCCAATTCTAATTCCTCTCCTTCGAGGACTATTTCAGTATTCTCTACCATTCCTCTGCCGCCGAGTTCCGGCGCCGTCGATGGTTCCGGGAGTTCCACTTCGGCTCGGAAACCGATCAGTCTCTGGCCGGGCATGCACCATTCGCCGGTGACCACCGCCCTTTGGGAAGCTAGGTCGAGTATCTTTGAGAGGTTGCTTGATCCTCCTAAAGATGCGCCGCCGCAGAGTGAATTGCTTACCAAAACGCCTTCTCAGAGTCGGACTATTGTAATTTATAATTTCTCTTCTGATTTTATTCTGAGAGAGCAGTATCGAGATCCGTGGAATGAGGTCAGAATCGGAAAGTTGCTTGAAGATCTTGATGCCTTGGCCGGTACCATCTCCGTTAGACACTGTTCTGATGATGATAGCACAACAAGACCACTTTTGCTAGTGACTGCCTCTGTTGACAGAATTGTTCTAAAGAAGCCAATTAGTGTTGATGCTGATTTGAAAATAGTTGGTTCAGTCATATGGGTTGGGCGATCCTCAATTGAGGTTCAGTTAGACATGATTCAGTCCACAGAAGACAGTGAAACAAAAGAAAAGTGGACAATCTTTAGTTATTGTTATATCAGCATTGCAGTACAAGCTCACTACCACTGTGATCGCATTCTCTTCAAAGGGCCTTTTCGATCTCATCTTCCAGTCATTAGGAATTGGACTCTGGCCCAAACTTTGAAGCATGAATCTTGGGCTAATTGCTCAAACCAGTCAGACTCGGTTGCTCTAACAGCAAACTTCATATTTGTGGCACGCGACTCTCAGACTGGGAAAGCTGCTTTGGTTAACAGACTTTCGCCAGAAACTGAGGAAGAAAAATTACTTTTTGAGGGAGCAGAAGCAAGGAGTAAATTAAGGAAAAGGAAGAATAGAGGTGATTTGTGGGGTTTGGATAATGGGTACATAAAAAGAATTGATGCTTTGCTAGCAGAGGGAAGAATCTTCTGTGACATGCCAGCCCTGGCAGACAGAGACAGCATTCTTCTCAGGGATACTCGCCTTGAAAACTCTTTGATTTGTCAACCACAACAAAGAAACATACATGGTCGGATTTTCGGAGGGTTTCTTATGCAACGAGCATTTGAGTTGGCTTTCTCAACAGCATATGTGTTTGCCGGAATGGTGCCTTACTTTTTTGAAGTTGATCATGTTGATTTCTTAAAACCTGTGGATGTTGGTGACTTTCTACGTTTCACATCATGTGTTCTTTACACAGAAGTCGACAATCCGGATCAGCCATTGATCAACATTGAAGTTGTTGCCCATGTTACTAGGCCCGAGATCAGGTCTAGCGAGGTGTCAAATACATTCTATTTCACTTTCACTGTGCGTCCGGAGGCAAAAGCTAAGGGAAACGGATTCAGACTTCGCAACGTCGTTCCAGCAACAGAAGAAGAAGCTCGTCGTATCATTGAGCGGATGGATGCCGAAAAAGATGCTAAGGGACTTGAGCAAGCAATCTGAATCAGGTCAGGCTTAGGCAGCCAACACAGTTTGTCTTGGTTTAAGAAAATCTTGTCGCATCCTTATTTTGAAATTCGGCTAAGTTATAGAAAACTGATCTTGTAGAATATTTGTGAAGGAAATCAATCTTATTCAATTACTGTTGGAGGGAGAAGAGCAATCAAATTATGTAATGGAGTATTGATTATTTAAGCCCTATTTGGTAATCTTTAGTTTTTAGTTTTTTAAAATTAAGCATATAAACATTCATTTCAACAAAAAAGTGTTGAAATAAAAATTGCTTTGTTATCTACTTTTTATCAATGTTTTCAAAACCAAG

Coding sequence (CDS)

ATGTTTGTTTATAAAGCAGAGGCAATGTGGAAGATCAAAAGCAAAGTCCAACACTCTGTTCAAGTTCAAGCTTTGAACTCTGTTTTTTCCCTCAAATCTTGGAGTTCAACCCCAAAACCCAAATTCAATTCCCAGTTCCAACGTTCATTTTCCATGGATTCCAATTCTAATTCCTCTCCTTCGAGGACTATTTCAGTATTCTCTACCATTCCTCTGCCGCCGAGTTCCGGCGCCGTCGATGGTTCCGGGAGTTCCACTTCGGCTCGGAAACCGATCAGTCTCTGGCCGGGCATGCACCATTCGCCGGTGACCACCGCCCTTTGGGAAGCTAGGTCGAGTATCTTTGAGAGGTTGCTTGATCCTCCTAAAGATGCGCCGCCGCAGAGTGAATTGCTTACCAAAACGCCTTCTCAGAGTCGGACTATTGTAATTTATAATTTCTCTTCTGATTTTATTCTGAGAGAGCAGTATCGAGATCCGTGGAATGAGGTCAGAATCGGAAAGTTGCTTGAAGATCTTGATGCCTTGGCCGGTACCATCTCCGTTAGACACTGTTCTGATGATGATAGCACAACAAGACCACTTTTGCTAGTGACTGCCTCTGTTGACAGAATTGTTCTAAAGAAGCCAATTAGTGTTGATGCTGATTTGAAAATAGTTGGTTCAGTCATATGGGTTGGGCGATCCTCAATTGAGGTTCAGTTAGACATGATTCAGTCCACAGAAGACAGTGAAACAAAAGAAAAGTGGACAATCTTTAGTTATTGTTATATCAGCATTGCAGTACAAGCTCACTACCACTGTGATCGCATTCTCTTCAAAGGGCCTTTTCGATCTCATCTTCCAGTCATTAGGAATTGGACTCTGGCCCAAACTTTGAAGCATGAATCTTGGGCTAATTGCTCAAACCAGTCAGACTCGGTTGCTCTAACAGCAAACTTCATATTTGTGGCACGCGACTCTCAGACTGGGAAAGCTGCTTTGGTTAACAGACTTTCGCCAGAAACTGAGGAAGAAAAATTACTTTTTGAGGGAGCAGAAGCAAGGAGTAAATTAAGGAAAAGGAAGAATAGAGGTGATTTGTGGGGTTTGGATAATGGGTACATAAAAAGAATTGATGCTTTGCTAGCAGAGGGAAGAATCTTCTGTGACATGCCAGCCCTGGCAGACAGAGACAGCATTCTTCTCAGGGATACTCGCCTTGAAAACTCTTTGATTTGTCAACCACAACAAAGAAACATACATGGTCGGATTTTCGGAGGGTTTCTTATGCAACGAGCATTTGAGTTGGCTTTCTCAACAGCATATGTGTTTGCCGGAATGGTGCCTTACTTTTTTGAAGTTGATCATGTTGATTTCTTAAAACCTGTGGATGTTGGTGACTTTCTACGTTTCACATCATGTGTTCTTTACACAGAAGTCGACAATCCGGATCAGCCATTGATCAACATTGAAGTTGTTGCCCATGTTACTAGGCCCGAGATCAGGTCTAGCGAGGTGTCAAATACATTCTATTTCACTTTCACTGTGCGTCCGGAGGCAAAAGCTAAGGGAAACGGATTCAGACTTCGCAACGTCGTTCCAGCAACAGAAGAAGAAGCTCGTCGTATCATTGAGCGGATGGATGCCGAAAAAGATGCTAAGGGACTTGAGCAAGCAATCTGA

Protein sequence

MFVYKAEAMWKIKSKVQHSVQVQALNSVFSLKSWSSTPKPKFNSQFQRSFSMDSNSNSSPSRTISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMDAEKDAKGLEQAI
Homology
BLAST of Lsi02G021840 vs. ExPASy Swiss-Prot
Match: S4TDL2 (Acyl-coenzyme A thioesterase 2, chloroplastic OS=Humulus lupulus OX=3486 GN=TE2 PE=2 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 4.2e-186
Identity = 348/490 (71.02%), Postives = 383/490 (78.16%), Query Frame = 0

Query: 57  NSSPSRTISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFE 116
           +SSP+  I+V ST    P  G      S++SARKPISLWPGM+HSPVT ALWEAR  IFE
Sbjct: 4   SSSPNHPITVVSTF-ASPFEGPPSVGDSNSSARKPISLWPGMYHSPVTNALWEARCKIFE 63

Query: 117 RLLDPPKDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDAL 176
           RLLDPPKDAPPQSELLTKTPSQSRT ++YNFS+D+ILREQYRDPWNEVRIGKLLEDLDAL
Sbjct: 64  RLLDPPKDAPPQSELLTKTPSQSRTTILYNFSTDYILREQYRDPWNEVRIGKLLEDLDAL 123

Query: 177 AGTISVRHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLD 236
           AGTISV+HCSDDDSTTRPLLLVTASVD+IVLKKPISVD DLKIVG+VIWVGRSSIE+QL+
Sbjct: 124 AGTISVKHCSDDDSTTRPLLLVTASVDKIVLKKPISVDIDLKIVGAVIWVGRSSIEIQLE 183

Query: 237 MIQSTEDSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHE 296
           + QST++                                                     
Sbjct: 184 VSQSTKEG---------------------------------------------------- 243

Query: 297 SWANCSNQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRK 356
                SN +D VAL+ANFIFVARDS+T KAA VNRLSPETE+EKLLF+ AEARS +RKRK
Sbjct: 244 -----SNAADDVALSANFIFVARDSKTAKAAPVNRLSPETEQEKLLFDEAEARSSMRKRK 303

Query: 357 NRGD--LWGLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNI 416
            RGD      +NG   R+  LLAEGRIFCDMPALADRDSILLRDTR ENSLICQPQQRNI
Sbjct: 304 -RGDQERREFENGEANRLQTLLAEGRIFCDMPALADRDSILLRDTRQENSLICQPQQRNI 363

Query: 417 HGRIFGGFLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEV 476
           HGRIFGGFL+ RAFELAFSTAY FAG+VPYF E+DHVDF +PVDVGDFLR  SCVLYTE+
Sbjct: 364 HGRIFGGFLLHRAFELAFSTAYAFAGLVPYFLEIDHVDFXRPVDVGDFLRLKSCVLYTEL 423

Query: 477 DNPDQPLINIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEA 536
            NPDQPLINIEVVAHVTRPE+RSSEVSNTFYFTFTVRPEAKA  NG+R+RNVVPATEEEA
Sbjct: 424 HNPDQPLINIEVVAHVTRPELRSSEVSNTFYFTFTVRPEAKATKNGYRIRNVVPATEEEA 434

Query: 537 RRIIERMDAE 545
           RRI+ERMDAE
Sbjct: 484 RRILERMDAE 434

BLAST of Lsi02G021840 vs. ExPASy Swiss-Prot
Match: S4TF94 (Acyl-coenzyme A thioesterase 4, mitochondrial OS=Humulus lupulus OX=3486 GN=TE4 PE=1 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.8e-153
Identity = 298/507 (58.78%), Postives = 366/507 (72.19%), Query Frame = 0

Query: 40  PKFNSQFQRSFSMDSNSNSSPSRTISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMH 99
           P       +SFS D +S   PS    V STI    +      +GS  S RKPISLWPGM 
Sbjct: 71  PPIRFTHTKSFSTDPSSLDFPSNQPPVVSTISSHHNISQPIDAGS--SIRKPISLWPGMF 130

Query: 100 HSPVTTALWEARSSIFERLLDPPKDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRD 159
           +SPVT ALWEARS++FE+  +P  D P QSEL+TK+P+QSRT ++YN SSD+ LRE YR+
Sbjct: 131 NSPVTNALWEARSNMFEKYGEPTADPPSQSELVTKSPAQSRTSILYNLSSDYALREHYRN 190

Query: 160 PWNEVRIGKLLEDLDALAGTISVRHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKI 219
           PWN +RIGKLLEDLDALAGTI+ +HC+++D  +RPLLLVTASVD++VLKKPIS+D DL I
Sbjct: 191 PWNMIRIGKLLEDLDALAGTIAFKHCTNEDGMSRPLLLVTASVDKMVLKKPISIDTDLSI 250

Query: 220 VGSVIWVGRSSIEVQLDMIQSTEDSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRS 279
           VG+V WVGRSS+E+QL ++Q+T                                      
Sbjct: 251 VGAVTWVGRSSMEIQLQVLQTT-------------------------------------- 310

Query: 280 HLPVIRNWTLAQTLKHESWANCSNQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEE 339
                          HES    S+ SDSV+L ANF FVARDS+TGK+A++N++SPET EE
Sbjct: 311 ---------------HES----SDPSDSVSLVANFTFVARDSKTGKSAVINQISPETGEE 370

Query: 340 KLLFEGAEARSKLR--KRKNRGDLWGLDNGYIKRIDALLAEGRIFCDMPALADRDSILLR 399
           KLL+  A+ R+K+R  KRK + DL  L+  YI+R++ALLAEGR+FCD+PALADR+SIL++
Sbjct: 371 KLLWREADERNKMRKMKRKIQKDL-ELEKQYIERLNALLAEGRVFCDLPALADRNSILMK 430

Query: 400 DTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPV 459
           DT LENS ICQPQQRNI+GRIFGGFLM+RA ELAFST Y FAG+V +F EVDHVDFL+PV
Sbjct: 431 DTCLENSFICQPQQRNIYGRIFGGFLMRRAVELAFSTTYSFAGVVTHFLEVDHVDFLRPV 490

Query: 460 DVGDFLRFTSCVLYTEVDNPDQPLINIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAK 519
           DVGDFLR  SCVLYTE+ NP +PLIN+EVVAHVTRPE+RSSEVSN FYFTF+V PEA   
Sbjct: 491 DVGDFLRLKSCVLYTELQNPTEPLINVEVVAHVTRPELRSSEVSNKFYFTFSVGPEAVK- 515

Query: 520 GNGFRLRNVVPATEEEARRIIERMDAE 545
            +G  +RNVVPATEEEARR++ERMDAE
Sbjct: 551 -DGLLVRNVVPATEEEARRVLERMDAE 515

BLAST of Lsi02G021840 vs. ExPASy Swiss-Prot
Match: Q9R0X4 (Acyl-coenzyme A thioesterase 9, mitochondrial OS=Mus musculus OX=10090 GN=Acot9 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.8e-35
Identity = 112/377 (29.71%), Postives = 171/377 (45.36%), Query Frame = 0

Query: 143 VIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDDDSTTRPLLLVTASV 202
           V+    +D  LR++Y    N VR G++LEDLD+L   +   H  +  +   PL +VT  V
Sbjct: 89  VLLPLGTDPELRDKYVTVQNTVRFGRILEDLDSLGVLVCYMHNHNHSTKMSPLSIVTVLV 148

Query: 203 DRI-VLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSETKEKWTIFSYCYISIA 262
           D+I + K  +S + D+K  G V WVG +S+EV++ M Q   D   ++ W +         
Sbjct: 149 DKIDMCKHSLSPEQDIKFTGHVSWVGNTSMEVKMKMFQLHND---EKYWPV--------- 208

Query: 263 VQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSDSVALTANFIFVARDS 322
                                                           L A F+ VARDS
Sbjct: 209 ------------------------------------------------LDATFVMVARDS 268

Query: 323 QTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGL-----DNGYIKRIDALL 382
           +    A VN L PE +EE+ LF+  E     R   +   L  +     +   I  +    
Sbjct: 269 ENKGPAFVNPLIPENKEEEELFKQGELNKSRRIAFSTSSLLKVAPSSEERNIIHELFLTT 328

Query: 383 AEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAY 442
            + +       +    ++ + DT+L++  IC PQ+RN+  RIFGGFLM++A+ELA++TA 
Sbjct: 329 LDPKTISFQSRILPPKAVWMEDTKLKSLDICHPQERNVFNRIFGGFLMRKAYELAWATAC 388

Query: 443 VFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEVVAHVTRPEIR 502
            F G  PY   VD + F KPV+VG  L  +S V +T+ DN     I + V + V+  + R
Sbjct: 389 SFGGSRPYVVTVDDIMFQKPVEVGSLLFLSSQVCFTQ-DN----YIQVRVHSEVSSLDSR 400

Query: 503 SSEVSNTFYFTFTVRPE 514
               +N F+FTF    E
Sbjct: 449 EHMTTNVFHFTFMSEKE 400

BLAST of Lsi02G021840 vs. ExPASy Swiss-Prot
Match: Q9Y305 (Acyl-coenzyme A thioesterase 9, mitochondrial OS=Homo sapiens OX=9606 GN=ACOT9 PE=1 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 1.3e-33
Identity = 118/400 (29.50%), Postives = 178/400 (44.50%), Query Frame = 0

Query: 125 APPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRH 184
           A  Q  L  +    S   V+    S+  LRE+Y    N VR G++LEDLD+L   I   H
Sbjct: 72  AKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTVRFGRILEDLDSLGVLICYMH 131

Query: 185 CSDDDSTTRPLLLVTASVDRI-VLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTED 244
                +   PL +VTA VD+I + KK +S + D+K  G V WVG++S+EV++ M Q   D
Sbjct: 132 NKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHVSWVGKTSMEVKMQMFQLHGD 191

Query: 245 SETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSN 304
                      +C                         PV                    
Sbjct: 192 ----------EFC-------------------------PV-------------------- 251

Query: 305 QSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAE----------ARSKLR 364
                 L A F+ VARDS+    A VN L PE+ EE+ LF   E          + S L+
Sbjct: 252 ------LDATFVMVARDSENKGPAFVNPLIPESPEEEELFRQGELNKGRRIAFSSTSLLK 311

Query: 365 KRKNRGDLWGLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRN 424
              +  +   +   ++  +D      R       +   +++ + +++L++  IC PQ+RN
Sbjct: 312 MAPSAEERTTIHEMFLSTLDPKTISFR-----SRVLPSNAVWMENSKLKSLEICHPQERN 371

Query: 425 IHGRIFGGFLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTE 484
           I  RIFGGFLM++A+ELA++TA  F G  P+   VD + F KPV+VG  L  +S V +T+
Sbjct: 372 IFNRIFGGFLMRKAYELAWATACSFGGSRPFVVAVDDIMFQKPVEVGSLLFLSSQVCFTQ 400

Query: 485 VDNPDQPLINIEVVAHVTRPEIRSSEVSNTFYFTFTVRPE 514
                   I + V + V   + +    +N F+FTF    E
Sbjct: 432 -----NNYIQVRVHSEVASLQEKQHTTTNVFHFTFMSEKE 400

BLAST of Lsi02G021840 vs. ExPASy Swiss-Prot
Match: Q32MW3 (Acyl-coenzyme A thioesterase 10, mitochondrial OS=Mus musculus OX=10090 GN=Acot10 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.7e-33
Identity = 108/377 (28.65%), Postives = 169/377 (44.83%), Query Frame = 0

Query: 143 VIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSDDDSTTRPLLLVTASV 202
           V+    +D  LR++Y    N VR G++LEDLD+L   +   H  +  +    L +VT  V
Sbjct: 89  VLLPLGTDPELRDKYVTVQNTVRFGRILEDLDSLGVLVCYMHNHNHSTNMSLLSIVTVLV 148

Query: 203 DRI-VLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSETKEKWTIFSYCYISIA 262
           D+I + K  +S + D+K  G V WVG +++EV++ M Q  +D                  
Sbjct: 149 DKIDMCKHSLSPEQDIKFTGHVSWVGNTTMEVKMKMFQLHDD------------------ 208

Query: 263 VQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSDSVALTANFIFVARDS 322
                            ++ PV                          L A F+ VA+DS
Sbjct: 209 ----------------ETYWPV--------------------------LDATFVMVAQDS 268

Query: 323 QTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGL-----DNGYIKRIDALL 382
           +  + A VN L PE +EE+ LF   E     R   +   L  +     +   I  +    
Sbjct: 269 ENKRPAFVNPLIPENKEEEELFTQGELNKSRRIAFSTSSLLKVAPSSEERNIIHELFLST 328

Query: 383 AEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRAFELAFSTAY 442
            + +       +    ++ + DT+L++  IC PQ+RN+  RIFGGFLM++A+ELA++TA 
Sbjct: 329 LDPKTISFQSRILPPKAVWMEDTKLKSLDICHPQERNVFNRIFGGFLMRKAYELAWATAC 388

Query: 443 VFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEVVAHVTRPEIR 502
            F G  PY   VD + F KPV+VG  L  +S V +T+ +      I + V + V   + R
Sbjct: 389 SFGGSRPYVVTVDDIMFQKPVEVGSLLFLSSQVCFTQGN-----YIQVRVHSEVFSLDSR 400

Query: 503 SSEVSNTFYFTFTVRPE 514
               +N F+FTF    E
Sbjct: 449 EHMTTNVFHFTFMSEKE 400

BLAST of Lsi02G021840 vs. ExPASy TrEMBL
Match: A0A1S3BGC8 (acyl-coenzyme A thioesterase 9, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103489713 PE=4 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.8e-241
Identity = 452/552 (81.88%), Postives = 466/552 (84.42%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSSTPKPKFNSQFQRSFSMDSNS------NSSPSR 68
           M KIK K      VQAL+SVF+LKSW+S PKP FN +FQR F MD+NS      NSSPSR
Sbjct: 1   MLKIKCK------VQALSSVFTLKSWTSIPKPSFNFKFQRLFYMDANSNSNSNFNSSPSR 60

Query: 69  TISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPP 128
           TISVFSTIPLPPSS AV+GSGSSTS+RKPI+LWPGMHHSPVT ALW+ARSSIFERLLDPP
Sbjct: 61  TISVFSTIPLPPSSDAVNGSGSSTSSRKPINLWPGMHHSPVTAALWDARSSIFERLLDPP 120

Query: 129 KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 188
           KDAPPQSELLTKTPSQSR IVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV
Sbjct: 121 KDAPPQSELLTKTPSQSRIIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 180

Query: 189 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTE 248
           RHCSDDDSTTRPLLLVTASVDRIVLKKPISV ADLKIVGSVIWVGRSSIEVQLDMIQ TE
Sbjct: 181 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVVADLKIVGSVIWVGRSSIEVQLDMIQGTE 240

Query: 249 DSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCS 308
           D                                                         CS
Sbjct: 241 D---------------------------------------------------------CS 300

Query: 309 NQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLW 368
           N+SDSVALTANFIFVARDSQTGKAALVNRL PETEEEKLLFEGAEARS LRKRKNRGD+W
Sbjct: 301 NKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLFEGAEARSNLRKRKNRGDMW 360

Query: 369 GLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 428
           GL+NGY+KRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF
Sbjct: 361 GLENGYLKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 420

Query: 429 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI 488
           LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPD PLI
Sbjct: 421 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDHPLI 480

Query: 489 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMD 548
           NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKA G GFRLRNVVPATEEEARRIIERMD
Sbjct: 481 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRLRNVVPATEEEARRIIERMD 489

Query: 549 AEKDAKGLEQAI 555
           AE+DAKGLEQAI
Sbjct: 541 AERDAKGLEQAI 489

BLAST of Lsi02G021840 vs. ExPASy TrEMBL
Match: A0A5D3D148 (Acyl-coenzyme A thioesterase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002180 PE=4 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.8e-241
Identity = 452/552 (81.88%), Postives = 466/552 (84.42%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSSTPKPKFNSQFQRSFSMDSNS------NSSPSR 68
           M KIK K      VQAL+SVF+LKSW+S PKP FN +FQR F MD+NS      NSSPSR
Sbjct: 1   MLKIKCK------VQALSSVFTLKSWTSIPKPSFNFKFQRLFYMDANSNSNSNFNSSPSR 60

Query: 69  TISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPP 128
           TISVFSTIPLPPSS AV+GSGSSTS+RKPI+LWPGMHHSPVT ALW+ARSSIFERLLDPP
Sbjct: 61  TISVFSTIPLPPSSDAVNGSGSSTSSRKPINLWPGMHHSPVTAALWDARSSIFERLLDPP 120

Query: 129 KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 188
           KDAPPQSELLTKTPSQSR IVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV
Sbjct: 121 KDAPPQSELLTKTPSQSRIIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 180

Query: 189 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTE 248
           RHCSDDDSTTRPLLLVTASVDRIVLKKPISV ADLKIVGSVIWVGRSSIEVQLDMIQ TE
Sbjct: 181 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVVADLKIVGSVIWVGRSSIEVQLDMIQGTE 240

Query: 249 DSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCS 308
           D                                                         CS
Sbjct: 241 D---------------------------------------------------------CS 300

Query: 309 NQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLW 368
           N+SDSVALTANFIFVARDSQTGKAALVNRL PETEEEKLLFEGAEARS LRKRKNRGD+W
Sbjct: 301 NKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLFEGAEARSNLRKRKNRGDMW 360

Query: 369 GLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 428
           GL+NGY+KRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF
Sbjct: 361 GLENGYLKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 420

Query: 429 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI 488
           LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPD PLI
Sbjct: 421 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDHPLI 480

Query: 489 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMD 548
           NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKA G GFRLRNVVPATEEEARRIIERMD
Sbjct: 481 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRLRNVVPATEEEARRIIERMD 489

Query: 549 AEKDAKGLEQAI 555
           AE+DAKGLEQAI
Sbjct: 541 AERDAKGLEQAI 489

BLAST of Lsi02G021840 vs. ExPASy TrEMBL
Match: A0A0A0KA74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G373540 PE=4 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 6.2e-241
Identity = 450/552 (81.52%), Postives = 466/552 (84.42%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSSTPKPKFNSQFQRSFSMDS------NSNSSPSR 68
           M KIK K      VQAL+SVF+L SW+  PKP+FN +FQR F MD+      NSNSSPSR
Sbjct: 1   MLKIKGK------VQALSSVFTLNSWNLIPKPRFNFKFQRLFYMDANSSSNPNSNSSPSR 60

Query: 69  TISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPP 128
           TISVFSTIPLPPSS AV+GSGSSTS+RKPI+LWPGMHHSPVT ALW+ARSSIFERLLDPP
Sbjct: 61  TISVFSTIPLPPSSDAVNGSGSSTSSRKPINLWPGMHHSPVTAALWDARSSIFERLLDPP 120

Query: 129 KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 188
           KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV
Sbjct: 121 KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 180

Query: 189 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTE 248
           RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQ T+
Sbjct: 181 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQGTK 240

Query: 249 DSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCS 308
           D                                                         CS
Sbjct: 241 D---------------------------------------------------------CS 300

Query: 309 NQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLW 368
           N+SDSVALTANFIFVARDSQTGKAALVNRL PETEEEKLLFEGAEARS LRKRKNRGD+W
Sbjct: 301 NKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLFEGAEARSNLRKRKNRGDMW 360

Query: 369 GLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 428
            L+NGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF
Sbjct: 361 ALENGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 420

Query: 429 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI 488
           LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI
Sbjct: 421 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI 480

Query: 489 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMD 548
           N+EVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKA G GFRLR+VVPATEEEARRIIERMD
Sbjct: 481 NVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRLRSVVPATEEEARRIIERMD 489

Query: 549 AEKDAKGLEQAI 555
           AEKDAKGLEQ I
Sbjct: 541 AEKDAKGLEQGI 489

BLAST of Lsi02G021840 vs. ExPASy TrEMBL
Match: A0A6J1GS66 (acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111456516 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 7.7e-223
Identity = 426/545 (78.17%), Postives = 443/545 (81.28%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSST-PKPKFNSQFQRSFSMDSNSNSSPSRTISVF 68
           MWKI++K      VQAL     +KSWSS+ PK   N QF R FS   +S SSPSRTISVF
Sbjct: 1   MWKIQTK------VQAL-----IKSWSSSIPKSSINFQFHRPFS-SMDSISSPSRTISVF 60

Query: 69  STIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 128
           STIPLPP S A D SG+ST +RKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP
Sbjct: 61  STIPLPPGSDAADASGNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 120

Query: 129 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSD 188
           QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSD
Sbjct: 121 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSD 180

Query: 189 DDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSETK 248
            DSTTRPLLLVTASVDRIVLKKPISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE     
Sbjct: 181 VDSTTRPLLLVTASVDRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE----- 240

Query: 249 EKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSDS 308
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 309 VALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGLDNG 368
           VALTANFIFVARDS+TGKAALVNR+ PETEEEKLLFEGAEARSKLRKRKNRG +WGL+NG
Sbjct: 301 VALTANFIFVARDSKTGKAALVNRILPETEEEKLLFEGAEARSKLRKRKNRGGMWGLENG 360

Query: 369 YIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 428
            IKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA
Sbjct: 361 DIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 420

Query: 429 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEVV 488
           FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTE+DNPDQPLIN+EVV
Sbjct: 421 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVV 468

Query: 489 AHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMDAEKDA 548
           AHVTRPEIRSSEVSNTFYFTFTVRPEAKA GNGFRLRNVVPATEEEARRIIERMDAEK  
Sbjct: 481 AHVTRPEIRSSEVSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDAEKML 468

Query: 549 KGLEQ 553
           + L +
Sbjct: 541 RDLSK 468

BLAST of Lsi02G021840 vs. ExPASy TrEMBL
Match: A0A6J1JVH9 (acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111488217 PE=4 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.8e-222
Identity = 425/545 (77.98%), Postives = 442/545 (81.10%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSW-SSTPKPKFNSQFQRSFSMDSNSNSSPSRTISVF 68
           MWKI+SK      VQAL     +KSW SS PK   N QF R FS   +S SSPSRTISVF
Sbjct: 1   MWKIQSK------VQAL-----IKSWNSSIPKSSINFQFHRPFS-SMDSISSPSRTISVF 60

Query: 69  STIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 128
           STIPLPP S A D SG+ST +RKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP
Sbjct: 61  STIPLPPGSDAADASGNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 120

Query: 129 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSD 188
           QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSD
Sbjct: 121 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSD 180

Query: 189 DDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSETK 248
            DSTTRPLLLVTASVDRIVLKKPISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE     
Sbjct: 181 VDSTTRPLLLVTASVDRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE----- 240

Query: 249 EKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSDS 308
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 309 VALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGLDNG 368
           VALTANFIFVARDS+TGKAALVNR+ PETE+EKLLFEGAEARSKLRKRKNRG +WGL+NG
Sbjct: 301 VALTANFIFVARDSKTGKAALVNRILPETEKEKLLFEGAEARSKLRKRKNRGGMWGLENG 360

Query: 369 YIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 428
            IKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA
Sbjct: 361 DIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 420

Query: 429 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEVV 488
           FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTE+DNPDQPLIN+EVV
Sbjct: 421 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVV 468

Query: 489 AHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMDAEKDA 548
           AHVTRPEIRSSEVSNTFYFTFTVRPEAKA GNGFRLRNVVPATEEEARRIIERMD+EK  
Sbjct: 481 AHVTRPEIRSSEVSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDSEKML 468

Query: 549 KGLEQ 553
           + L +
Sbjct: 541 RDLSK 468

BLAST of Lsi02G021840 vs. NCBI nr
Match: XP_038899833.1 (LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase 9, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 862.8 bits (2228), Expect = 1.6e-246
Identity = 460/546 (84.25%), Postives = 470/546 (86.08%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSSTPKPKFNSQFQRSFSM--DSNSNSSPSRTISV 68
           MWKIKSKVQHS  VQALNSVFSLKSWSS PKP+FN QF+ SFSM  +SNSNSSPSRTISV
Sbjct: 1   MWKIKSKVQHS--VQALNSVFSLKSWSSIPKPRFNFQFKHSFSMNSNSNSNSSPSRTISV 60

Query: 69  FSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAP 128
           FSTI LPPSS AVDGSGSSTS+RKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAP
Sbjct: 61  FSTITLPPSSDAVDGSGSSTSSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAP 120

Query: 129 PQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCS 188
           PQSEL TKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCS
Sbjct: 121 PQSELRTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCS 180

Query: 189 DDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSET 248
           DDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQ TED   
Sbjct: 181 DDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQGTED--- 240

Query: 249 KEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSD 308
                                                                 CSN+SD
Sbjct: 241 ------------------------------------------------------CSNKSD 300

Query: 309 SVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGLDN 368
           SVALTANFIFVARDSQTGKAALVNRL PETEEEKLLFEGAEARS LRKRKNRGD+WGL+N
Sbjct: 301 SVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLFEGAEARSNLRKRKNRGDMWGLEN 360

Query: 369 GYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQR 428
           GYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQR
Sbjct: 361 GYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQR 420

Query: 429 AFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEV 488
           AFELAFSTAYVFAGMVPYF+EVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEV
Sbjct: 421 AFELAFSTAYVFAGMVPYFYEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEV 480

Query: 489 VAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMDAEKD 548
           VAHVTRPEIRSSEVSNTFYFTFTVRPEAKA G GFRLRNVVPATEEEARRIIERMDAEK 
Sbjct: 481 VAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRLRNVVPATEEEARRIIERMDAEKM 487

Query: 549 AKGLEQ 553
            + L +
Sbjct: 541 LRDLSK 487

BLAST of Lsi02G021840 vs. NCBI nr
Match: XP_008447218.1 (PREDICTED: acyl-coenzyme A thioesterase 9, mitochondrial [Cucumis melo] >XP_008447219.1 PREDICTED: acyl-coenzyme A thioesterase 9, mitochondrial [Cucumis melo] >KAA0041987.1 acyl-coenzyme A thioesterase 9 [Cucumis melo var. makuwa] >TYK17923.1 acyl-coenzyme A thioesterase 9 [Cucumis melo var. makuwa])

HSP 1 Score: 844.3 bits (2180), Expect = 5.8e-241
Identity = 452/552 (81.88%), Postives = 466/552 (84.42%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSSTPKPKFNSQFQRSFSMDSNS------NSSPSR 68
           M KIK K      VQAL+SVF+LKSW+S PKP FN +FQR F MD+NS      NSSPSR
Sbjct: 1   MLKIKCK------VQALSSVFTLKSWTSIPKPSFNFKFQRLFYMDANSNSNSNFNSSPSR 60

Query: 69  TISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPP 128
           TISVFSTIPLPPSS AV+GSGSSTS+RKPI+LWPGMHHSPVT ALW+ARSSIFERLLDPP
Sbjct: 61  TISVFSTIPLPPSSDAVNGSGSSTSSRKPINLWPGMHHSPVTAALWDARSSIFERLLDPP 120

Query: 129 KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 188
           KDAPPQSELLTKTPSQSR IVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV
Sbjct: 121 KDAPPQSELLTKTPSQSRIIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 180

Query: 189 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTE 248
           RHCSDDDSTTRPLLLVTASVDRIVLKKPISV ADLKIVGSVIWVGRSSIEVQLDMIQ TE
Sbjct: 181 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVVADLKIVGSVIWVGRSSIEVQLDMIQGTE 240

Query: 249 DSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCS 308
           D                                                         CS
Sbjct: 241 D---------------------------------------------------------CS 300

Query: 309 NQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLW 368
           N+SDSVALTANFIFVARDSQTGKAALVNRL PETEEEKLLFEGAEARS LRKRKNRGD+W
Sbjct: 301 NKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLFEGAEARSNLRKRKNRGDMW 360

Query: 369 GLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 428
           GL+NGY+KRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF
Sbjct: 361 GLENGYLKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 420

Query: 429 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI 488
           LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPD PLI
Sbjct: 421 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDHPLI 480

Query: 489 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMD 548
           NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKA G GFRLRNVVPATEEEARRIIERMD
Sbjct: 481 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRLRNVVPATEEEARRIIERMD 489

Query: 549 AEKDAKGLEQAI 555
           AE+DAKGLEQAI
Sbjct: 541 AERDAKGLEQAI 489

BLAST of Lsi02G021840 vs. NCBI nr
Match: XP_004148940.2 (acyl-coenzyme A thioesterase 9, mitochondrial [Cucumis sativus] >KGN44711.1 hypothetical protein Csa_016240 [Cucumis sativus])

HSP 1 Score: 843.2 bits (2177), Expect = 1.3e-240
Identity = 450/552 (81.52%), Postives = 466/552 (84.42%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSSTPKPKFNSQFQRSFSMDS------NSNSSPSR 68
           M KIK K      VQAL+SVF+L SW+  PKP+FN +FQR F MD+      NSNSSPSR
Sbjct: 1   MLKIKGK------VQALSSVFTLNSWNLIPKPRFNFKFQRLFYMDANSSSNPNSNSSPSR 60

Query: 69  TISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPP 128
           TISVFSTIPLPPSS AV+GSGSSTS+RKPI+LWPGMHHSPVT ALW+ARSSIFERLLDPP
Sbjct: 61  TISVFSTIPLPPSSDAVNGSGSSTSSRKPINLWPGMHHSPVTAALWDARSSIFERLLDPP 120

Query: 129 KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 188
           KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV
Sbjct: 121 KDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISV 180

Query: 189 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTE 248
           RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQ T+
Sbjct: 181 RHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQGTK 240

Query: 249 DSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCS 308
           D                                                         CS
Sbjct: 241 D---------------------------------------------------------CS 300

Query: 309 NQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLW 368
           N+SDSVALTANFIFVARDSQTGKAALVNRL PETEEEKLLFEGAEARS LRKRKNRGD+W
Sbjct: 301 NKSDSVALTANFIFVARDSQTGKAALVNRLLPETEEEKLLFEGAEARSNLRKRKNRGDMW 360

Query: 369 GLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 428
            L+NGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF
Sbjct: 361 ALENGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGF 420

Query: 429 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI 488
           LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI
Sbjct: 421 LMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLI 480

Query: 489 NIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMD 548
           N+EVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKA G GFRLR+VVPATEEEARRIIERMD
Sbjct: 481 NVEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKANGKGFRLRSVVPATEEEARRIIERMD 489

Query: 549 AEKDAKGLEQAI 555
           AEKDAKGLEQ I
Sbjct: 541 AEKDAKGLEQGI 489

BLAST of Lsi02G021840 vs. NCBI nr
Match: XP_023548726.1 (LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 794.3 bits (2050), Expect = 6.9e-226
Identity = 431/547 (78.79%), Postives = 448/547 (81.90%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSST-PKPKFNSQFQRSFSMDSNSNSSPSRTISVF 68
           MWKI++K      VQAL     +KSWSS+ PK   N QF R FS   +S SSPSRTISVF
Sbjct: 1   MWKIQTK------VQAL-----IKSWSSSIPKSSINFQFHRPFS-SMDSISSPSRTISVF 60

Query: 69  STIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 128
           STIPLPP S A D SG+ST +RKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP
Sbjct: 61  STIPLPPGSDAADASGNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 120

Query: 129 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSD 188
           QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSD
Sbjct: 121 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSD 180

Query: 189 DDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSETK 248
            DSTTRPLLLVTASVDRIVLKKPISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE     
Sbjct: 181 VDSTTRPLLLVTASVDRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE----- 240

Query: 249 EKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSDS 308
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 309 VALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGLDNG 368
           VALTANFIFVARDS+TGKAALVNR+ PET+EEKLLFEGAEARSKLRKRKNRG +WGL+NG
Sbjct: 301 VALTANFIFVARDSKTGKAALVNRILPETDEEKLLFEGAEARSKLRKRKNRGGMWGLENG 360

Query: 369 YIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 428
            IKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA
Sbjct: 361 DIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 420

Query: 429 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEVV 488
           FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTE+DNPDQPLIN+EVV
Sbjct: 421 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVV 470

Query: 489 AHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMDAEKDA 548
           AHVTRPEIRSSEVSNTFYFTFTVRPEAKA GNGFRLRNVVPATEEEARRIIERMDAE DA
Sbjct: 481 AHVTRPEIRSSEVSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDAEXDA 470

Query: 549 KGLEQAI 555
           +GLEQAI
Sbjct: 541 QGLEQAI 470

BLAST of Lsi02G021840 vs. NCBI nr
Match: XP_022954174.1 (acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 783.1 bits (2021), Expect = 1.6e-222
Identity = 426/545 (78.17%), Postives = 443/545 (81.28%), Query Frame = 0

Query: 9   MWKIKSKVQHSVQVQALNSVFSLKSWSST-PKPKFNSQFQRSFSMDSNSNSSPSRTISVF 68
           MWKI++K      VQAL     +KSWSS+ PK   N QF R FS   +S SSPSRTISVF
Sbjct: 1   MWKIQTK------VQAL-----IKSWSSSIPKSSINFQFHRPFS-SMDSISSPSRTISVF 60

Query: 69  STIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 128
           STIPLPP S A D SG+ST +RKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP
Sbjct: 61  STIPLPPGSDAADASGNSTLSRKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDAPP 120

Query: 129 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHCSD 188
           QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNE RIGKLLEDLDALAGTISVRHCSD
Sbjct: 121 QSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEARIGKLLEDLDALAGTISVRHCSD 180

Query: 189 DDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSETK 248
            DSTTRPLLLVTASVDRIVLKKPISVDADLK+VGSVIWVG+SSIEVQLDMIQSTE     
Sbjct: 181 VDSTTRPLLLVTASVDRIVLKKPISVDADLKVVGSVIWVGKSSIEVQLDMIQSTE----- 240

Query: 249 EKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQSDS 308
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 309 VALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDLWGLDNG 368
           VALTANFIFVARDS+TGKAALVNR+ PETEEEKLLFEGAEARSKLRKRKNRG +WGL+NG
Sbjct: 301 VALTANFIFVARDSKTGKAALVNRILPETEEEKLLFEGAEARSKLRKRKNRGGMWGLENG 360

Query: 369 YIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 428
            IKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA
Sbjct: 361 DIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLMQRA 420

Query: 429 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINIEVV 488
           FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTE+DNPDQPLIN+EVV
Sbjct: 421 FELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTELDNPDQPLINVEVV 468

Query: 489 AHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMDAEKDA 548
           AHVTRPEIRSSEVSNTFYFTFTVRPEAKA GNGFRLRNVVPATEEEARRIIERMDAEK  
Sbjct: 481 AHVTRPEIRSSEVSNTFYFTFTVRPEAKANGNGFRLRNVVPATEEEARRIIERMDAEKML 468

Query: 549 KGLEQ 553
           + L +
Sbjct: 541 RDLSK 468

BLAST of Lsi02G021840 vs. TAIR 10
Match: AT5G48370.1 (Thioesterase/thiol ester dehydrase-isomerase superfamily protein )

HSP 1 Score: 629.4 bits (1622), Expect = 2.7e-180
Identity = 341/493 (69.17%), Postives = 375/493 (76.06%), Query Frame = 0

Query: 62  RTISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDP 121
           R ISV ST   P        S +S   RKP+SLWPGM+HSPVTTALWEARS IFE LLDP
Sbjct: 5   RPISVVSTFASP--------SSTSDPTRKPLSLWPGMYHSPVTTALWEARSKIFESLLDP 64

Query: 122 PKDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTIS 181
           PKDAPPQS+LLT+TPS SRT + Y FS+DFILREQYRDPWNEVRIG LLEDLDALAGTIS
Sbjct: 65  PKDAPPQSQLLTRTPSHSRTTIFYPFSTDFILREQYRDPWNEVRIGILLEDLDALAGTIS 124

Query: 182 VRHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQST 241
           V+HCSDDDSTTRPLLLVTASV +IVLKKPI VD DLKIV SVIWVGRSSIE+QL+++Q  
Sbjct: 125 VKHCSDDDSTTRPLLLVTASVHKIVLKKPICVDIDLKIVASVIWVGRSSIEIQLEVMQ-- 184

Query: 242 EDSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANC 301
             SE K+             V+A                                     
Sbjct: 185 --SELKD-------------VKA------------------------------------- 244

Query: 302 SNQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDL 361
              SDSVALTANFIFVARDS+TGKAA +NRLSPETE EKLLFE AEAR+ LRK+K  GD 
Sbjct: 245 --SSDSVALTANFIFVARDSKTGKAAPINRLSPETEVEKLLFEEAEARNNLRKKKRGGDR 304

Query: 362 WGLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGG 421
              D+G  K+++A LAEGRIF DMPALADR+SILL+DTRLENSLICQPQQRNIHGRIFGG
Sbjct: 305 REFDHGECKKLEAWLAEGRIFSDMPALADRNSILLKDTRLENSLICQPQQRNIHGRIFGG 364

Query: 422 FLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPL 481
           FLM RAFELAFSTAY FAG+VPYF EVDHVDFL+PVDVGDFLRF SCVLYT++D  D PL
Sbjct: 365 FLMHRAFELAFSTAYTFAGLVPYFLEVDHVDFLRPVDVGDFLRFKSCVLYTQLDKQDCPL 424

Query: 482 INIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERM 541
           INIEVVAHVT PEIRSSEVSNTFYF FTVRPEAKA+ NGF+LRNVVPATEEEAR I+ERM
Sbjct: 425 INIEVVAHVTSPEIRSSEVSNTFYFKFTVRPEAKARNNGFKLRNVVPATEEEARHILERM 433

Query: 542 DAEKDAKGLEQAI 555
           DAE      +Q +
Sbjct: 485 DAEALKSSKQQCV 433

BLAST of Lsi02G021840 vs. TAIR 10
Match: AT5G48370.2 (Thioesterase/thiol ester dehydrase-isomerase superfamily protein )

HSP 1 Score: 629.4 bits (1622), Expect = 2.7e-180
Identity = 338/493 (68.56%), Postives = 370/493 (75.05%), Query Frame = 0

Query: 62  RTISVFSTIPLPPSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDP 121
           R ISV ST   P        S +S   RKP+SLWPGM+HSPVTTALWEARS IFE LLDP
Sbjct: 5   RPISVVSTFASP--------SSTSDPTRKPLSLWPGMYHSPVTTALWEARSKIFESLLDP 64

Query: 122 PKDAPPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTIS 181
           PKDAPPQS+LLT+TPS SRT + Y FS+DFILREQYRDPWNEVRIG LLEDLDALAGTIS
Sbjct: 65  PKDAPPQSQLLTRTPSHSRTTIFYPFSTDFILREQYRDPWNEVRIGILLEDLDALAGTIS 124

Query: 182 VRHCSDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQST 241
           V+HCSDDDSTTRPLLLVTASV +IVLKKPI VD DLKIV SVIWVGRSSIE+QL+++QS 
Sbjct: 125 VKHCSDDDSTTRPLLLVTASVHKIVLKKPICVDIDLKIVASVIWVGRSSIEIQLEVMQSE 184

Query: 242 EDSETKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANC 301
                                                                     N 
Sbjct: 185 ---------------------------------------------------------LNV 244

Query: 302 SNQSDSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKNRGDL 361
              SDSVALTANFIFVARDS+TGKAA +NRLSPETE EKLLFE AEAR+ LRK+K  GD 
Sbjct: 245 KASSDSVALTANFIFVARDSKTGKAAPINRLSPETEVEKLLFEEAEARNNLRKKKRGGDR 304

Query: 362 WGLDNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGG 421
              D+G  K+++A LAEGRIF DMPALADR+SILL+DTRLENSLICQPQQRNIHGRIFGG
Sbjct: 305 REFDHGECKKLEAWLAEGRIFSDMPALADRNSILLKDTRLENSLICQPQQRNIHGRIFGG 364

Query: 422 FLMQRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPL 481
           FLM RAFELAFSTAY FAG+VPYF EVDHVDFL+PVDVGDFLRF SCVLYT++D  D PL
Sbjct: 365 FLMHRAFELAFSTAYTFAGLVPYFLEVDHVDFLRPVDVGDFLRFKSCVLYTQLDKQDCPL 424

Query: 482 INIEVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERM 541
           INIEVVAHVT PEIRSSEVSNTFYF FTVRPEAKA+ NGF+LRNVVPATEEEAR I+ERM
Sbjct: 425 INIEVVAHVTSPEIRSSEVSNTFYFKFTVRPEAKARNNGFKLRNVVPATEEEARHILERM 432

Query: 542 DAEKDAKGLEQAI 555
           DAE      +Q +
Sbjct: 485 DAEALKSSKQQCV 432

BLAST of Lsi02G021840 vs. TAIR 10
Match: AT2G30720.1 (Thioesterase/thiol ester dehydrase-isomerase superfamily protein )

HSP 1 Score: 495.4 bits (1274), Expect = 6.1e-140
Identity = 274/481 (56.96%), Postives = 338/481 (70.27%), Query Frame = 0

Query: 68  STIPLP--PSSGAVDGSGSSTSARKPISLWPGMHHSPVTTALWEARSSIFERLLDPPKDA 127
           ST+P    P   ++D   + +S RKPISLWPGM+HSPVT ALWEAR ++FE    P  D 
Sbjct: 37  STVPSDENPDQNSID---AGSSMRKPISLWPGMYHSPVTNALWEARRNMFE---IPTGDD 96

Query: 128 PPQSELLTKTPSQSRTIVIYNFSSDFILREQYRDPWNEVRIGKLLEDLDALAGTISVRHC 187
             QS+L  K+PS+SRT ++Y FSSDF+LREQYR+PWNE+R GKL+EDLDALAGTIS +HC
Sbjct: 97  ASQSKLTAKSPSRSRTSILYKFSSDFVLREQYRNPWNEIRTGKLVEDLDALAGTISFKHC 156

Query: 188 SDDDSTTRPLLLVTASVDRIVLKKPISVDADLKIVGSVIWVGRSSIEVQLDMIQSTEDSE 247
              DS+ R ++LVTASVDRI++K+PI VD DL IVG+V WVGRSS+E+QL ++Q  +   
Sbjct: 157 G-GDSSARSMILVTASVDRIIMKRPIRVDTDLSIVGAVTWVGRSSMEMQLQVLQIQD--- 216

Query: 248 TKEKWTIFSYCYISIAVQAHYHCDRILFKGPFRSHLPVIRNWTLAQTLKHESWANCSNQS 307
                                                                   +N S
Sbjct: 217 -------------------------------------------------------TNNSS 276

Query: 308 DSVALTANFIFVARDSQTGKAALVNRLSPETEEEKLLFEGAEARSKLRKRKN-RGDLWGL 367
           +SVAL ANF FVARD+QTGK+A +N++ PETE EK L++ AE R+KLRK+K  +G     
Sbjct: 277 ESVALEANFTFVARDAQTGKSAPINQVVPETEHEKFLWKEAEERNKLRKQKRAQGKEEHE 336

Query: 368 DNGYIKRIDALLAEGRIFCDMPALADRDSILLRDTRLENSLICQPQQRNIHGRIFGGFLM 427
               ++R++ LLAEGR+F DMPALADR+SIL++DT  ENSLICQPQQRNIHGRIFGGFLM
Sbjct: 337 KLKDLERLNELLAEGRVFLDMPALADRNSILIKDTSHENSLICQPQQRNIHGRIFGGFLM 396

Query: 428 QRAFELAFSTAYVFAGMVPYFFEVDHVDFLKPVDVGDFLRFTSCVLYTEVDNPDQPLINI 487
           ++AFELAFS AY FAG+ P F EVD VDF+KPVDVG+FLRF S VLYTE  +  +PLINI
Sbjct: 397 RKAFELAFSNAYTFAGVSPRFLEVDRVDFIKPVDVGNFLRFKSRVLYTEATSSAEPLINI 450

Query: 488 EVVAHVTRPEIRSSEVSNTFYFTFTVRPEAKAKGNGFRLRNVVPATEEEARRIIERMDAE 546
           EVVAHVT PE+RSSEVSN FYFTF+VRPEA    +G ++RNVVPATEEEARR+IERMDAE
Sbjct: 457 EVVAHVTSPELRSSEVSNRFYFTFSVRPEAMK--DGLKIRNVVPATEEEARRVIERMDAE 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
S4TDL24.2e-18671.02Acyl-coenzyme A thioesterase 2, chloroplastic OS=Humulus lupulus OX=3486 GN=TE2 ... [more]
S4TF942.8e-15358.78Acyl-coenzyme A thioesterase 4, mitochondrial OS=Humulus lupulus OX=3486 GN=TE4 ... [more]
Q9R0X41.8e-3529.71Acyl-coenzyme A thioesterase 9, mitochondrial OS=Mus musculus OX=10090 GN=Acot9 ... [more]
Q9Y3051.3e-3329.50Acyl-coenzyme A thioesterase 9, mitochondrial OS=Homo sapiens OX=9606 GN=ACOT9 P... [more]
Q32MW33.7e-3328.65Acyl-coenzyme A thioesterase 10, mitochondrial OS=Mus musculus OX=10090 GN=Acot1... [more]
Match NameE-valueIdentityDescription
A0A1S3BGC82.8e-24181.88acyl-coenzyme A thioesterase 9, mitochondrial OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5D3D1482.8e-24181.88Acyl-coenzyme A thioesterase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0KA746.2e-24181.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G373540 PE=4 SV=1[more]
A0A6J1GS667.7e-22378.17acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1JVH93.8e-22277.98acyl-coenzyme A thioesterase 9, mitochondrial-like OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
XP_038899833.11.6e-24684.25LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase 9, mitochondrial-like [Beninca... [more]
XP_008447218.15.8e-24181.88PREDICTED: acyl-coenzyme A thioesterase 9, mitochondrial [Cucumis melo] >XP_0084... [more]
XP_004148940.21.3e-24081.52acyl-coenzyme A thioesterase 9, mitochondrial [Cucumis sativus] >KGN44711.1 hypo... [more]
XP_023548726.16.9e-22678.79LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbi... [more]
XP_022954174.11.6e-22278.17acyl-coenzyme A thioesterase 9, mitochondrial-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G48370.12.7e-18069.17Thioesterase/thiol ester dehydrase-isomerase superfamily protein [more]
AT5G48370.22.7e-18068.56Thioesterase/thiol ester dehydrase-isomerase superfamily protein [more]
AT2G30720.16.1e-14056.96Thioesterase/thiol ester dehydrase-isomerase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 389..552
e-value: 1.0E-39
score: 137.9
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 287..357
e-value: 1.6E-11
score: 46.4
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 151..269
e-value: 3.9E-27
score: 96.9
NoneNo IPR availablePANTHERPTHR12655ACYL-COA THIOESTERASEcoord: 55..545
NoneNo IPR availablePANTHERPTHR12655:SF3BNAA04G17790D PROTEINcoord: 55..545
NoneNo IPR availableCDDcd03442BFIT_BACHcoord: 140..252
e-value: 2.69507E-24
score: 96.0989
NoneNo IPR availableCDDcd03442BFIT_BACHcoord: 398..513
e-value: 1.82479E-30
score: 113.433
IPR033120Hotdog acyl-CoA thioesterase (ACOT)-type domainPROSITEPS51770HOTDOG_ACOTcoord: 143..265
score: 19.689472
IPR033120Hotdog acyl-CoA thioesterase (ACOT)-type domainPROSITEPS51770HOTDOG_ACOTcoord: 397..514
score: 28.102312
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 395..541
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 150..248
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 301..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G021840.1Lsi02G021840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006637 acyl-CoA metabolic process
molecular_function GO:0047617 acyl-CoA hydrolase activity