Lsi02G021650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G021650
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionSodium Bile acid symporter family
Locationchr02: 28082590 .. 28087748 (-)
RNA-Seq ExpressionLsi02G021650
SyntenyLsi02G021650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGAAAGTTTTAAAATATAGAGACGCAATTGAAATTAAAGAGAGGGACTAATTTGCAATTTCTTAAAGAAAAACAAAATAAAATTAAAATATTGAGACTGTCAGCACTTTCTTCCCAACACTTGGACGGATATTTCTCTCTCTTTGGCCTCCACTGTTCCCTTTTTCTTCCCCCTCGTTATCATCTATTGTACGAACCAACAAACCTCACCGGCGGCCAACCATTCACTTTCCGGCGACAATACTCCGGCGACTTCACTGTTTACACTCCCTTCCCATTTCCATGTCCGCCATAGCTCTTTTCTCTTCTCCGGTTCCCTTTCTTTCTCAATCGCCGATCAAACCTAGTAGAAAACCAAAATTGCATTCATCCGATTGCCACTGCCTTTCGATTTCGTGTAGCTTCACCAGATTGTGCCCTTCTTCTCACTGTTCGACGTCGAAGAATTGGAATCCGGTGTGTAATGGAGCTTTGTCGACGTTTGCTTGCTCGACGCCGTTCATTGGGAGAGTTGGTTCGCGGTTTCGTCAGGGGAATTTCTCGTTGCTGTCCTTTGGATTGAACTCCAATGCGGCGGCGGAGGCTGCTGGTGGCGATTCGTCTCAGCTTTTGTCGGCGTTGCTTCCTTTTGTGGTGGCTGCTACTGGGATTGCGGCTTTGGCTTGGCCTTCTACTTTTACTTGGTAAATTTGGATCTTACTGATTTAATTATTGTAATGTAATTCCCAATTTTCAATGTTAGCCACTTAGCCCTTTTTGGTTTAATGTTTTAAATTAATTTTTAATAAATTCTCTCTCTCCCTTGTTCTTGATTGAAGTCAGCATTCCATTCTTGTCTCTGGTAAATGATTTTTATTAATTTACTATTGTCTTTTTATAGGCTAAATATTAGATAGTACTCTGAATTCTTACCTATACTTTAAAAAATTACAATATTTACCCAAAACATTTCAAAACTAAATTGGAGCTAATATTTGAGACCTAAACGATATGGCTGTGATCTAGAGCAGAATTCTGGATCACCATTTAGATCGTGGTCATGCTGTTTTAAATTCCAATTCTATTTGAAATTCAGGAACATCTCTAATAATTTTTTAAATATGTACAAAAGTTCAAGGGTGATATTGCAACTTTTGGGAGTATAAAAATATTTTTGAAACAGAAGTCAAAGTTAAGTGGTATTTTCTATAAATTACCTTTTTAATTAAATAATTAAATAATAATAATAATTATTAATCTTATTCATCCTAAAAGGTTGAAACTGCTATATCCTTGGTACTAAATATAGCTGGTGTTGTACTCAATGTATATATATATATGTATATATTTATTGATTAATAATCTGCCACTGCTAGTCAAATATCTAATAAATAACGGTTTCTTTCATTTTTCAACCAGTCAACTTTCTGGGTGTTTTTTTTCTTCCTTAAATTCGTTTTAAATTTTGAATGTCATTTTCTGTATTTCTTTACTTTATGAGTTTCAGTTTTGGAACTTTCAATTTGCAGGGTTCACAAAGGAGCTATATGATGGCACATAGCTTTCCAGTTACCCCTTTTATATGTTGCTATCTTATTTAGAAGATTAGATCTTTCAATAAAACTAACTATTAGATCTTTCAATAAAACTAACTGTTATATTGATATGTTGCTGTTTGAGGTAGTGCATGTAGTTTATACAAATATATCAACCAAATTTGAATTTGTGAAGAGTTTCTTATGAAGTTCTTGACTTGGCATTTTGCAGGGTTCCTAAGGATCTCTATGCCCCAGCTCTTGGTGGGATAATGTTATCTATTGGGATTAAGCTCTCAATTAATGATTTTGCTCTAGCAATTAAGAGGTTAGACATATGAATTGCCAATAATATCAACTGCATTTGAAAACTTGAGTCTGTTCATGAGAATCATTTCATTTTGTTTGTTATTATTATTTGCAGGCCTCTACCACTTTCTGTTGGGTTTATTGCTCAGTATGTTTTGAAACCAGCTCTTGGTGTATTGATAGCAAGAGCATTTGGCATATCTCCAATGTTCTATGCAGGATTTCTTCTCACAGCCTGTGTTGGAGGGGCCCAGCTGTCAAGTTATGCCAGTTTCTTGAGTAAAGGAGATATTGCCTTGAGTATTCTCCTTACCAGCTTCACCACCATCTCATCCGTGCTTCTAACACCTCTATTGACTGGCATTCTGATCGGTTCGGTTGTTCCGGTTGATGCAGTTGCCATGTCAAAGTCAATATTGCAGGTAATGGTACTTCAATGAATAATGTATAGTAGATATGATTCGTGGATTTCAAATAAAAAAATCCTACTACGATTGTAGTACATACTTCTTTCGTTAATTTGTTTAGTAAGAAACCGAGCTTTCATTAATAAAAATGGAAGAATGTACAAGGACATCCGAAAACCAAATCTACAAAAGGAGTCCTCAACTACAGGAAATGACTCCAACCTAAAAGAATACAACCAAGTTGATAATTAAGAAAAATCCGATTGATGGACACCAATAAAGACCTATTAAATCTCACCACCTCCAAAAACCTCTCCGCTACTCTAAAAATCCTACTATGCTCTCGAGCTGCATACACCACATGATAACAAAACAACATATCTACCGCAAAACTCTTCCCTTGTTCCGGAAAGGATACTCCTTCCCGAGCATGTACTAGAGTAGTATTTTCTTGAAAAAAAATTTCCCTACTTTGTAGTTGATGTCCAAAATTATGCACGCAAAAAGATAGTGCAGAAAGGTCTTGATATTTTGGGTTTCTCATTATTTAGGCATCTAGTTATATGAAGGAGTTTTTCTAGTCTTCATATAAAGAGACTCTTCTTAGCAAATGAGTTAGTTAAACAGAAACTTCTTTTATGAAGATTTTCTTGTGTGTTCTTTTCCTTCTACAATTCTTCAGTTAAGTTTTTTACTGTTTTTGCGCATATTTTCCTATTTCTGGGCTGTAACTAAATTTTATCTCCTCTTTACGATCAAGAAATGGTGTATTTTAAGGTTTCAAGGGCATTCTGCACTATACTGATATAATCATTAATGTTTGGTTATTAATGTTCTGGCATTGTTATATAGGTTGTTCTTCTTCCAGTCGCTCTTGGACTTGTCCTTAATTCTTATGCAAAGCCGATCGTAGCCGTTCTTCAGCCTGTGATGCCATTTGTTGCAATGATATGTACTTCATTGTGCATCGGTAGCCCTCTTGCTATAAACAGAAGCCAAATTTTGTCAATAGATGGAGCCCGGTTGGTTCTTCCAGTATTAGCATTTCATACTATGGCATTCACTTTGGGATACTGGGCGTCGAAAATCCCAATCGTCAGGTAAATTTTATATCTCTTTACATAAAATCCCATCTATTTTGATGAACTTTGGGGATCAGTATCAGTTCCTTCAGAATGATCTTTTTGTAGCAGAAATCAGACTTCATATAATTGATCCCACCAGCATTCTATCAGGCATGAAAATAGTAGTATCTCTCTATTGATAAAATGGTTACAAAAGTTTAGCAGGGCAGGTAGAAACCATCTGATCTTTGCTTTGATCAATTGCCTTCGCCTTGAATCTTTTTAGAAAACTGTTAAAGCATGTGACTATAATCAGTGAAGGCTCTTCAACCCTTTATTCACATGAAAACTATCTACTGTCATAATAATATTGCTTCCTTTTCTGTTCAAGTGGGTCATAGATAACAACACTTTTTGCCATAGTACATGAAATAGGTAGCATCAATCAAATCTTCAGAGTATGCCTTGTTGGGAGCAGTTTATAAAAATTATTTTTAAGCTTAATGATCAAAAGTAGAAGCGCTATTTGAGAGAAAATAACTCTGTATTTTAGAATAAAATTTAGCGAAAACGTGTTTGCTAGAACCTTTGAAAATTGTTTATTCTTGTATTTTATTCTAGTTTATTTCCACTGATCTGTTTTTCAGAACAACTGAAGCCATTTTTTAATCCCCAAACTATGAGTCTTCTATTATTAATTTCCAGTGCATAACAACTATAACCTTTACAAGCCATCAAAAATCATGTTCCAAAATTCTCAAAACATAAGTAGAAAACTATTTTTTCAAATAGCTCCCAAGCAACAAAATTACATGAAAGTCAAATATTCAAGTATCTGTTGGTGGCTTAAGAATTGCATAAAAACTTTTATCGCCTTTGTCTAAACAAACTTTCTCATTCCTATAGAGATAATGTTTTGAATTTTCATGACTATACTGTGTTTTAGGGAGTAGTCATGTCATTTTGTTTGTTACACTGGTTTGGAATGAATTTGCATTGATTTCCAACTAGAGGTAGTAGGAAGTCGGGACTCATAGCTCCTGATTTCGGGTAGAAGAAAGCTTAATAAATGAAAAACATTGATGTCCTCCGACATTAGATGCACAGTTCCCCTTGTAAATGAATGAGGTTGGCTCAATACAAAGGTTAACGAAAACTATATTAGACTGAATATTGAATTCAAACAAATATTTTGGTTTCAAAATATGATGAACATTGCTTGATACAATGTTGTGGATGATGTCACAGGCAAGAAGAAGAAGCTAGCAGAACAATTTCACTATGCACTGGGATGCAGAGCTCCACTCTAGCAGGACTTCTTGCTACTCAGTTCCTCGGGAGCACTCAGGCCGTTCCACCCGCGTGTTCGGTTGTTGCCATGGCCATCATGGGCCTTTGTCTAGCTTCTTTCTGGGGCAGTGGCTCTAGAATTAGAGACTTGCCATCTCTTTTGATACAGAAAACCAGTTCAATTGTCAGTGCTTCTTGAATCTGCTTGAATTAGCAACTCTCCCATCTCTTAAACTGGTTGAAATGTTTCACATTGCAACAGCAAAAAGTCCATTTGAAGGCAAAGGAAAAAAGTGCTTCAACCAGCACATGTGATTCTCAGGCACCATATAGAAGATAGCAGGAAATAAATTTACTTGTGTAATGTTCATATGATAACAGGAAATCTCATGGGCGTTACCATGAGAATGAGGCAGAATAATATAAAGTATCATGATAGTTTTCAAATAAAAGCAAGAAAAAAAAAAGGTTGTTTGTCATCATTTCAGTTTATTATGATGTATTCTTTATTTGTACAGAAAATATAGTAAATACCCCACTTTTTAATCATTAAAAAAATGGTCATCTTTTGCTTC

mRNA sequence

TTGAAAGTTTTAAAATATAGAGACGCAATTGAAATTAAAGAGAGGGACTAATTTGCAATTTCTTAAAGAAAAACAAAATAAAATTAAAATATTGAGACTGTCAGCACTTTCTTCCCAACACTTGGACGGATATTTCTCTCTCTTTGGCCTCCACTGTTCCCTTTTTCTTCCCCCTCGTTATCATCTATTGTACGAACCAACAAACCTCACCGGCGGCCAACCATTCACTTTCCGGCGACAATACTCCGGCGACTTCACTGTTTACACTCCCTTCCCATTTCCATGTCCGCCATAGCTCTTTTCTCTTCTCCGGTTCCCTTTCTTTCTCAATCGCCGATCAAACCTAGTAGAAAACCAAAATTGCATTCATCCGATTGCCACTGCCTTTCGATTTCGTGTAGCTTCACCAGATTGTGCCCTTCTTCTCACTGTTCGACGTCGAAGAATTGGAATCCGGTGTGTAATGGAGCTTTGTCGACGTTTGCTTGCTCGACGCCGTTCATTGGGAGAGTTGGTTCGCGGTTTCGTCAGGGGAATTTCTCGTTGCTGTCCTTTGGATTGAACTCCAATGCGGCGGCGGAGGCTGCTGGTGGCGATTCGTCTCAGCTTTTGTCGGCGTTGCTTCCTTTTGTGGTGGCTGCTACTGGGATTGCGGCTTTGGCTTGGCCTTCTACTTTTACTTGGGTTCCTAAGGATCTCTATGCCCCAGCTCTTGGTGGGATAATGTTATCTATTGGGATTAAGCTCTCAATTAATGATTTTGCTCTAGCAATTAAGAGGCCTCTACCACTTTCTGTTGGGTTTATTGCTCAGTATGTTTTGAAACCAGCTCTTGGTGTATTGATAGCAAGAGCATTTGGCATATCTCCAATGTTCTATGCAGGATTTCTTCTCACAGCCTGTGTTGGAGGGGCCCAGCTGTCAAGTTATGCCAGTTTCTTGAGTAAAGGAGATATTGCCTTGAGTATTCTCCTTACCAGCTTCACCACCATCTCATCCGTGCTTCTAACACCTCTATTGACTGGCATTCTGATCGGTTCGGTTGTTCCGGTTGATGCAGTTGCCATGTCAAAGTCAATATTGCAGGTTGTTCTTCTTCCAGTCGCTCTTGGACTTGTCCTTAATTCTTATGCAAAGCCGATCGTAGCCGTTCTTCAGCCTGTGATGCCATTTGTTGCAATGATATGTACTTCATTGTGCATCGGTAGCCCTCTTGCTATAAACAGAAGCCAAATTTTGTCAATAGATGGAGCCCGGTTGGTTCTTCCAGTATTAGCATTTCATACTATGGCATTCACTTTGGGATACTGGGCGTCGAAAATCCCAATCGTCAGGCAAGAAGAAGAAGCTAGCAGAACAATTTCACTATGCACTGGGATGCAGAGCTCCACTCTAGCAGGACTTCTTGCTACTCAGTTCCTCGGGAGCACTCAGGCCGTTCCACCCGCGTGTTCGGTTGTTGCCATGGCCATCATGGGCCTTTGTCTAGCTTCTTTCTGGGGCAGTGGCTCTAGAATTAGAGACTTGCCATCTCTTTTGATACAGAAAACCAGTTCAATTGTCAGTGCTTCTTGAATCTGCTTGAATTAGCAACTCTCCCATCTCTTAAACTGGTTGAAATGTTTCACATTGCAACAGCAAAAAGTCCATTTGAAGGCAAAGGAAAAAAGTGCTTCAACCAGCACATGTGATTCTCAGGCACCATATAGAAGATAGCAGGAAATAAATTTACTTGTGTAATGTTCATATGATAACAGGAAATCTCATGGGCGTTACCATGAGAATGAGGCAGAATAATATAAAGTATCATGATAGTTTTCAAATAAAAGCAAGAAAAAAAAAAGGTTGTTTGTCATCATTTCAGTTTATTATGATGTATTCTTTATTTGTACAGAAAATATAGTAAATACCCCACTTTTTAATCATTAAAAAAATGGTCATCTTTTGCTTC

Coding sequence (CDS)

ATGTCCGCCATAGCTCTTTTCTCTTCTCCGGTTCCCTTTCTTTCTCAATCGCCGATCAAACCTAGTAGAAAACCAAAATTGCATTCATCCGATTGCCACTGCCTTTCGATTTCGTGTAGCTTCACCAGATTGTGCCCTTCTTCTCACTGTTCGACGTCGAAGAATTGGAATCCGGTGTGTAATGGAGCTTTGTCGACGTTTGCTTGCTCGACGCCGTTCATTGGGAGAGTTGGTTCGCGGTTTCGTCAGGGGAATTTCTCGTTGCTGTCCTTTGGATTGAACTCCAATGCGGCGGCGGAGGCTGCTGGTGGCGATTCGTCTCAGCTTTTGTCGGCGTTGCTTCCTTTTGTGGTGGCTGCTACTGGGATTGCGGCTTTGGCTTGGCCTTCTACTTTTACTTGGGTTCCTAAGGATCTCTATGCCCCAGCTCTTGGTGGGATAATGTTATCTATTGGGATTAAGCTCTCAATTAATGATTTTGCTCTAGCAATTAAGAGGCCTCTACCACTTTCTGTTGGGTTTATTGCTCAGTATGTTTTGAAACCAGCTCTTGGTGTATTGATAGCAAGAGCATTTGGCATATCTCCAATGTTCTATGCAGGATTTCTTCTCACAGCCTGTGTTGGAGGGGCCCAGCTGTCAAGTTATGCCAGTTTCTTGAGTAAAGGAGATATTGCCTTGAGTATTCTCCTTACCAGCTTCACCACCATCTCATCCGTGCTTCTAACACCTCTATTGACTGGCATTCTGATCGGTTCGGTTGTTCCGGTTGATGCAGTTGCCATGTCAAAGTCAATATTGCAGGTTGTTCTTCTTCCAGTCGCTCTTGGACTTGTCCTTAATTCTTATGCAAAGCCGATCGTAGCCGTTCTTCAGCCTGTGATGCCATTTGTTGCAATGATATGTACTTCATTGTGCATCGGTAGCCCTCTTGCTATAAACAGAAGCCAAATTTTGTCAATAGATGGAGCCCGGTTGGTTCTTCCAGTATTAGCATTTCATACTATGGCATTCACTTTGGGATACTGGGCGTCGAAAATCCCAATCGTCAGGCAAGAAGAAGAAGCTAGCAGAACAATTTCACTATGCACTGGGATGCAGAGCTCCACTCTAGCAGGACTTCTTGCTACTCAGTTCCTCGGGAGCACTCAGGCCGTTCCACCCGCGTGTTCGGTTGTTGCCATGGCCATCATGGGCCTTTGTCTAGCTTCTTTCTGGGGCAGTGGCTCTAGAATTAGAGACTTGCCATCTCTTTTGATACAGAAAACCAGTTCAATTGTCAGTGCTTCTTGA

Protein sequence

MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVCNGALSTFACSTPFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLSALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPSLLIQKTSSIVSAS
Homology
BLAST of Lsi02G021650 vs. ExPASy Swiss-Prot
Match: Q8RXE8 (Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS3 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.3e-137
Identity = 280/439 (63.78%), Postives = 337/439 (76.77%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSF---TRLCPSSHCSTSKNW- 60
           M+ IA  S P P + +  +      + H+      S +C F   +    S H S+  N+ 
Sbjct: 1   MTLIASLSLPPPAIQRQSLS-----EFHNLPAKSQSFNCQFRSSSSPLSSLHSSSLSNFL 60

Query: 61  ----NPVCNGALSTFACS-TPFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLS 120
                   +G +   ACS TPF+GRVG  +R GN SLLSF   ++   +A   DSSQ  S
Sbjct: 61  DFRLRRRNSGLVPVVACSTTPFMGRVGLHWRDGNMSLLSFCGGTDVTEKA---DSSQFWS 120

Query: 121 ALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLS 180
           ALLPFVVA T +AAL++P +FTWV KDLYAPALGGIMLSIGI+LS++DFALA KRP+PLS
Sbjct: 121 ALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVPLS 180

Query: 181 VGFIAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILL 240
           VGF+AQYVLKP LGVL+A AFG+   FYAGF+LT CV GAQLSSYAS LSK D+A+SILL
Sbjct: 181 VGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVAMSILL 240

Query: 241 TSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVL 300
           TS TTI+SV+ TPLL+G+LIGSVVPVDAVAMSKSILQVVL+P+ LGLVLN+YAKP+V +L
Sbjct: 241 TSSTTIASVIFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKPVVTLL 300

Query: 301 QPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVR 360
           QPVMPFVAM+CTSLCIGSPL+INRSQILS +G  L++P++ FH +AF LGYW SKIP +R
Sbjct: 301 QPVMPFVAMVCTSLCIGSPLSINRSQILSAEGLGLIVPIVTFHAVAFALGYWFSKIPGLR 360

Query: 361 QEEEASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSR 420
           QEEE SRTISLCTGMQSSTLAGLLA+QFLGS+QAVP ACSVV MAIMGLCLASFWG+G R
Sbjct: 361 QEEEVSRTISLCTGMQSSTLAGLLASQFLGSSQAVPAACSVVVMAIMGLCLASFWGNGFR 420

Query: 421 IRDLPSLLIQKTSSIVSAS 431
           IRD+ SL   +++   + S
Sbjct: 421 IRDVLSLSTPQSTGYTAES 431

BLAST of Lsi02G021650 vs. ExPASy Swiss-Prot
Match: Q6K739 (Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS3 PE=2 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.3e-129
Identity = 255/375 (68.00%), Postives = 309/375 (82.40%), Query Frame = 0

Query: 56  WNPVCNGALSTFACSTPFIGRVGSRFRQGNFSLLSFGLNS-NAAAEAAGGDSSQLLSALL 115
           W      A +TF CS P +GRVG   R+G   LLSF     ++ + AA GD SQ LSALL
Sbjct: 50  WRRPRRVAPTTF-CSAPSLGRVGWPRREGAAWLLSFRAGPVSSPSSAAAGDPSQALSALL 109

Query: 116 PFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGF 175
           P VVAAT +AAL  P+TF+WV K+ YAPALGGIMLSIGIKLSI+DFALA KRP+PL++G+
Sbjct: 110 PLVVAATAVAALGNPATFSWVSKEYYAPALGGIMLSIGIKLSIDDFALAFKRPVPLTIGY 169

Query: 176 IAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSF 235
           +AQY++KP +GVLIARAFG+   F+AGF+LT CV GAQLSSYASFLSKGD+ALSILLTS 
Sbjct: 170 MAQYIVKPLMGVLIARAFGMPSAFFAGFVLTCCVSGAQLSSYASFLSKGDVALSILLTSC 229

Query: 236 TTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPV 295
           +TISSV++TP+LTG+LIGSVVPVD +AM+KSILQVVL+PV LGL+LN+YAK +V V+QPV
Sbjct: 230 STISSVVVTPVLTGLLIGSVVPVDGIAMAKSILQVVLVPVTLGLLLNTYAKAVVNVIQPV 289

Query: 296 MPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEE 355
           MPFVAM+CTSLCIGSPLAINRS+ILS +G  L+LP++ FH  AF +GYW SK+P++RQEE
Sbjct: 290 MPFVAMLCTSLCIGSPLAINRSKILSSEGFLLLLPIVTFHIAAFIVGYWISKLPMLRQEE 349

Query: 356 EASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRD 415
              RTIS+CTGMQSSTLAGLLATQFLGS+QAVP ACSVV MAI GL LAS+WG+G RIRD
Sbjct: 350 PVCRTISVCTGMQSSTLAGLLATQFLGSSQAVPAACSVVIMAIFGLTLASYWGNGLRIRD 409

Query: 416 LPSLLIQKTSSIVSA 430
           + S  + + S+ VS+
Sbjct: 410 IGSRFVPQASAGVSS 423

BLAST of Lsi02G021650 vs. ExPASy Swiss-Prot
Match: B8BDK4 (Probable sodium/metabolite cotransporter BASS5, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=BASS5 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.8e-51
Identity = 129/312 (41.35%), Postives = 188/312 (60.26%), Query Frame = 0

Query: 111 SALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPL 170
           + ++P VV  + I AL +P +FTW     YAPALG +M ++G+  S+ DF  AI+RP  +
Sbjct: 92  NTIIPHVVLGSTILALVYPPSFTWFTTRYYAPALGFLMFAVGVNSSVKDFIEAIQRPDAI 151

Query: 171 SVGFIAQYVLKPAL----GVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIA 230
           + G++ Q+++KP L    G L    F +     AG +L +CV GAQLS+YA+FL+   +A
Sbjct: 152 AAGYVGQFIIKPFLGFLFGTLAVTIFNLPTALGAGIMLVSCVSGAQLSNYATFLTDPHMA 211

Query: 231 -LSILLTSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAK 290
            LSI++TS +T ++V +TP L+  LIG  +PVD   M  SI+Q+V+ P+A GL+LN Y  
Sbjct: 212 PLSIVMTSLSTATAVFVTPTLSYFLIGKKLPVDVKGMMSSIVQIVVAPIAAGLLLNRYLP 271

Query: 291 PIVAVLQPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGY--- 350
            + + +QP +P +++  T+LC+GSPLAIN   +LS  G   VL + AFHT +F  GY   
Sbjct: 272 RLCSAIQPFLPPLSVFVTALCVGSPLAINIKAVLSPFGLATVLLLFAFHTSSFIAGYHLA 331

Query: 351 --WASKIPIVRQEEEASRTISLCTGMQSSTLAGLLATQFL-GSTQAVPPACSVVAMAIMG 410
             W  +   V+      RT+S  TGMQSS LA  LA +F       VPPA SVV M++MG
Sbjct: 332 GTWFRESADVK---ALQRTVSFETGMQSSLLALALANRFFPDPLVGVPPAISVVLMSLMG 391

Query: 411 LCLASFWGSGSR 412
             L   W   ++
Sbjct: 392 FALVMVWSKRTK 400

BLAST of Lsi02G021650 vs. ExPASy Swiss-Prot
Match: Q650U0 (Probable sodium/metabolite cotransporter BASS5, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS5 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.8e-51
Identity = 129/312 (41.35%), Postives = 188/312 (60.26%), Query Frame = 0

Query: 111 SALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPL 170
           + ++P VV  + I AL +P +FTW     YAPALG +M ++G+  S+ DF  AI+RP  +
Sbjct: 92  NTIIPHVVLGSTILALVYPPSFTWFTTRYYAPALGFLMFAVGVNSSVKDFIEAIQRPDAI 151

Query: 171 SVGFIAQYVLKPAL----GVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIA 230
           + G++ Q+++KP L    G L    F +     AG +L +CV GAQLS+YA+FL+   +A
Sbjct: 152 AAGYVGQFIIKPFLGFLFGTLAVTIFNLPTALGAGIMLVSCVSGAQLSNYATFLTDPHMA 211

Query: 231 -LSILLTSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAK 290
            LSI++TS +T ++V +TP L+  LIG  +PVD   M  SI+Q+V+ P+A GL+LN Y  
Sbjct: 212 PLSIVMTSLSTATAVFVTPTLSYFLIGKKLPVDVKGMMSSIVQIVVAPIAAGLLLNRYLP 271

Query: 291 PIVAVLQPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGY--- 350
            + + +QP +P +++  T+LC+GSPLAIN   +LS  G   VL + AFHT +F  GY   
Sbjct: 272 RLCSAIQPFLPPLSVFVTALCVGSPLAINIKAVLSPFGLATVLLLFAFHTSSFIAGYHLA 331

Query: 351 --WASKIPIVRQEEEASRTISLCTGMQSSTLAGLLATQFL-GSTQAVPPACSVVAMAIMG 410
             W  +   V+      RT+S  TGMQSS LA  LA +F       VPPA SVV M++MG
Sbjct: 332 GTWFRESADVK---ALQRTVSFETGMQSSLLALALANRFFPDPLVGVPPAISVVLMSLMG 391

Query: 411 LCLASFWGSGSR 412
             L   W   ++
Sbjct: 392 FALVMVWSKRTK 400

BLAST of Lsi02G021650 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.1e-51
Identity = 124/300 (41.33%), Postives = 180/300 (60.00%), Query Frame = 0

Query: 108 QLLSALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRP 167
           +LL+ L P  V    +  +  PS  TW+  DL+   LG +MLS+G+ L+  DF   ++ P
Sbjct: 99  ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNP 158

Query: 168 LPLSVGFIAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIAL 227
             + VGF+AQY++KP LG LIA    +S     G +L +C  G Q S+ A+++SKG++AL
Sbjct: 159 WTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVAL 218

Query: 228 SILLTSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPI 287
           S+L+T+ +TI ++++TPLLT +L G +VPVDA  ++ S  QVVL+P  +G++ N +    
Sbjct: 219 SVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKF 278

Query: 288 VAVLQPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKI 347
            + +  V P + +I T+L   SP+    + +L   GA+L+LPV   H  AF +GYW SK 
Sbjct: 279 TSKIITVTPLIGVILTTLLCASPIG-QVADVLKTQGAQLILPVALLHAAAFAIGYWISKF 338

Query: 348 PIVRQEEEASRTISLCTGMQSSTLAGLLATQ-FLGSTQAVPPACSVVAMAIMGLCLASFW 407
                 E  SRTIS+  GMQSS L  LLA + F     AVP A SVV MA+ G  LA FW
Sbjct: 339 SF---GESTSRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFW 394

BLAST of Lsi02G021650 vs. ExPASy TrEMBL
Match: A0A5D3D222 (Putative sodium/metabolite cotransporter BASS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002340 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 3.9e-206
Identity = 394/433 (90.99%), Postives = 408/433 (94.23%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MS I L SSPVPFLSQSPIKPSRKPK  SSDCHCLSISC+FTRL PSSHCSTSKN  PVC
Sbjct: 1   MSPIPLCSSPVPFLSQSPIKPSRKPKFFSSDCHCLSISCTFTRLWPSSHCSTSKNLKPVC 60

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAA--EAAGGDSSQLLSALLPFV 120
           NGALSTFACST PFIGRVGSR R GNFSLLSFGLNSNAAA  E AGGDSSQ  S LLPFV
Sbjct: 61  NGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAEEGAGGDSSQFFSGLLPFV 120

Query: 121 VAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQ 180
           VAATGI+AL  PSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALA+KRP+PLS+GF+AQ
Sbjct: 121 VAATGISALTCPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSIGFVAQ 180

Query: 181 YVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240
           YVLKPALGVLIARAFGISP+FYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI
Sbjct: 181 YVLKPALGVLIARAFGISPLFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240

Query: 241 SSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPF 300
           SSVLLTPLLTGILIGSVVPVDA AMSKSILQVVLLPVALGLVLNSYAKPIVA++QPVMPF
Sbjct: 241 SSVLLTPLLTGILIGSVVPVDAAAMSKSILQVVLLPVALGLVLNSYAKPIVAIIQPVMPF 300

Query: 301 VAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEAS 360
           VA+ICTSLCIGSPLAINRSQILSI GA+LVLPVLAFH MAFTLGYWASKIPI+RQEEEAS
Sbjct: 301 VAIICTSLCIGSPLAINRSQILSIHGAQLVLPVLAFHAMAFTLGYWASKIPIIRQEEEAS 360

Query: 361 RTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPS 420
           RTISLCTGMQSSTLAGLLATQFLGST AVPPACSVVAMAIMGLCLASFWGSGS+IRDLPS
Sbjct: 361 RTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPS 420

Query: 421 LLIQKTSSIVSAS 431
           LL+QKTSSI  AS
Sbjct: 421 LLVQKTSSIAGAS 433

BLAST of Lsi02G021650 vs. ExPASy TrEMBL
Match: A0A1S3BGX9 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489720 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 5.1e-206
Identity = 394/433 (90.99%), Postives = 408/433 (94.23%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MSAI L SSPVPFLSQSPIKPSRKPK  SSDCHCLSISC+FTRL P SHCSTSKN  PVC
Sbjct: 1   MSAIPLCSSPVPFLSQSPIKPSRKPKFFSSDCHCLSISCTFTRLWPFSHCSTSKNLKPVC 60

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAA--EAAGGDSSQLLSALLPFV 120
           NGALSTFACST PFIGRVGSR R GNFSLLSFGLNSNAAA  E AGGDSSQ  S LLPFV
Sbjct: 61  NGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAEEGAGGDSSQFFSGLLPFV 120

Query: 121 VAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQ 180
           VAATGI+AL  PSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALA+KRP+PLS+GF+AQ
Sbjct: 121 VAATGISALTCPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSIGFVAQ 180

Query: 181 YVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240
           YVLKPALGVLIARAFGISP+FYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI
Sbjct: 181 YVLKPALGVLIARAFGISPLFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240

Query: 241 SSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPF 300
           SSVLLTPLLTGILIGSVVPVDA AMSKSILQVVLLPVALGLVLNSYAKPIVA++QPVMPF
Sbjct: 241 SSVLLTPLLTGILIGSVVPVDAAAMSKSILQVVLLPVALGLVLNSYAKPIVAIIQPVMPF 300

Query: 301 VAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEAS 360
           VA+ICTSLCIGSPLAINRSQILSI GA+LVLPVLAFH MAFTLGYWASKIPI+RQEEEAS
Sbjct: 301 VAIICTSLCIGSPLAINRSQILSIHGAQLVLPVLAFHAMAFTLGYWASKIPIIRQEEEAS 360

Query: 361 RTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPS 420
           RTISLCTGMQSSTLAGLLATQFLGST AVPPACSVVAMAIMGLCLASFWGSGS+IRDLPS
Sbjct: 361 RTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPS 420

Query: 421 LLIQKTSSIVSAS 431
           LL+QKTSSI  AS
Sbjct: 421 LLVQKTSSIAGAS 433

BLAST of Lsi02G021650 vs. ExPASy TrEMBL
Match: A0A6J1JWY1 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488209 PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.4e-203
Identity = 394/431 (91.42%), Postives = 408/431 (94.66%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MSAIAL SSPVPF SQ PIKP RKPKL+SSDC CLS SC+ TRL  SS+CSTSKNW+ V 
Sbjct: 1   MSAIALCSSPVPFPSQLPIKPRRKPKLYSSDCLCLSFSCNLTRLW-SSYCSTSKNWSTVR 60

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLSALLPFVVA 120
           NGA+STFACST PF+GR+GSR RQGNFSLLSFGLNSNA AEA  GDSSQLLSALLPFVVA
Sbjct: 61  NGAVSTFACSTSPFVGRIGSRIRQGNFSLLSFGLNSNAEAEATDGDSSQLLSALLPFVVA 120

Query: 121 ATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV 180
           AT IAAL  PSTFTWV KD YAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV
Sbjct: 121 ATAIAALTRPSTFTWVSKDFYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV 180

Query: 181 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTISS 240
           LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALS+LLTSFTTISS
Sbjct: 181 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSVLLTSFTTISS 240

Query: 241 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVA 300
           VLLTPLLTGILIGSVVPVDAVAMSKSILQVVL+P++LGLVLNSYAKPIVA+LQPVMPFVA
Sbjct: 241 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLVPISLGLVLNSYAKPIVALLQPVMPFVA 300

Query: 301 MICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEASRT 360
           MICTSLCIGSPLAINRSQILSIDGARLV PVL FHTMAFTLGYWASKIPIVRQEEEASRT
Sbjct: 301 MICTSLCIGSPLAINRSQILSIDGARLVFPVLVFHTMAFTLGYWASKIPIVRQEEEASRT 360

Query: 361 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPSLL 420
           ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGS+IRDLPSLL
Sbjct: 361 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSLL 420

Query: 421 IQKTSSIVSAS 431
           +QKTSSIVSAS
Sbjct: 421 MQKTSSIVSAS 430

BLAST of Lsi02G021650 vs. ExPASy TrEMBL
Match: A0A6J1GQ81 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456499 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 9.9e-202
Identity = 393/431 (91.18%), Postives = 406/431 (94.20%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MSAIAL SSPVPF SQ PIKP RKPKL+SSDC CLS SC+ TRL  SSHCSTSKNW+ V 
Sbjct: 1   MSAIALCSSPVPFPSQLPIKPRRKPKLYSSDCLCLSFSCNLTRLW-SSHCSTSKNWSTVR 60

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLSALLPFVVA 120
           NGA+STFACST  F GR+GSR RQGN SLLSFGLNSNA AEA  GDSSQLLSALLPFVVA
Sbjct: 61  NGAVSTFACSTSSFAGRIGSRIRQGNSSLLSFGLNSNAEAEATDGDSSQLLSALLPFVVA 120

Query: 121 ATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV 180
           AT IAAL  PSTFTWV KD YAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV
Sbjct: 121 ATAIAALTRPSTFTWVSKDFYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV 180

Query: 181 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTISS 240
           LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALS+LLTSFTTISS
Sbjct: 181 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSVLLTSFTTISS 240

Query: 241 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVA 300
           VLLTPLLTGILIGSVVPVDAVAMSKSILQVVL+P++LGLVLNSYAKPIVA+LQPVMPFVA
Sbjct: 241 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLVPISLGLVLNSYAKPIVALLQPVMPFVA 300

Query: 301 MICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEASRT 360
           MICTSLCIGSPLAINRSQILSIDGARLV PVLAFHTMAFTLGYWASKIPIVRQEEEASRT
Sbjct: 301 MICTSLCIGSPLAINRSQILSIDGARLVFPVLAFHTMAFTLGYWASKIPIVRQEEEASRT 360

Query: 361 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPSLL 420
           ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGS+IRDLPSLL
Sbjct: 361 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSLL 420

Query: 421 IQKTSSIVSAS 431
           ++KTSSIVSAS
Sbjct: 421 MKKTSSIVSAS 430

BLAST of Lsi02G021650 vs. ExPASy TrEMBL
Match: A0A6J1DPN9 (probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023064 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 6.9e-187
Identity = 372/430 (86.51%), Postives = 389/430 (90.47%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQ--SPIKPSRKPKLHSSDCHCL---SISCSFTRLCPSSHCSTSKN 60
           M AI L SSPVP L Q  SPI  + K K HSS C  +   SIS S +RL  SSHCSTSKN
Sbjct: 1   MFAIPLCSSPVPLLPQPPSPINHA-KSKFHSSHCSYVSVSSISSSQSRLWSSSHCSTSKN 60

Query: 61  WNPVCNGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLSALL 120
                NGAL +FACST PF+GRVGSR RQGNFSLLSFGLNS+AAA+  GGDSSQLLSALL
Sbjct: 61  CRTASNGALFSFACSTSPFVGRVGSRIRQGNFSLLSFGLNSHAAADGGGGDSSQLLSALL 120

Query: 121 PFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGF 180
           PFVVAAT IAALA PSTFTWVPKDLYAPALGGIMLSIGIKLSI+DFALAIKRPLPLSVGF
Sbjct: 121 PFVVAATAIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSIDDFALAIKRPLPLSVGF 180

Query: 181 IAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSF 240
           I QY LKPALG+LIARA GISPMFYAGFLLTACVGGAQLSSYASFLSKGD+ALSILLTSF
Sbjct: 181 ITQYGLKPALGILIARACGISPMFYAGFLLTACVGGAQLSSYASFLSKGDVALSILLTSF 240

Query: 241 TTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPV 300
           TTISSVLLTPLLTG+LIGSVVPVDAVAMSKSILQVVLLPV LGLVLNSYAKPIVA++QPV
Sbjct: 241 TTISSVLLTPLLTGVLIGSVVPVDAVAMSKSILQVVLLPVTLGLVLNSYAKPIVAIIQPV 300

Query: 301 MPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEE 360
           MPFVAMICTSLCIGSPLAINRSQILSIDG RLV PVLAFHT+AFT GYWASKIPI+RQEE
Sbjct: 301 MPFVAMICTSLCIGSPLAINRSQILSIDGVRLVFPVLAFHTLAFTFGYWASKIPIIRQEE 360

Query: 361 EASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRD 420
           EASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGS+IRD
Sbjct: 361 EASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRD 420

Query: 421 LPSLLIQKTS 425
           LPSLLIQKT+
Sbjct: 421 LPSLLIQKTN 429

BLAST of Lsi02G021650 vs. NCBI nr
Match: XP_038899090.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Benincasa hispida])

HSP 1 Score: 764.2 bits (1972), Expect = 5.9e-217
Identity = 415/431 (96.29%), Postives = 421/431 (97.68%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MSAI L+SSPVPFLSQSP KPSRKPKL+SSDCHCLSISCSFTRL PSSHCSTSKNW PVC
Sbjct: 69  MSAITLYSSPVPFLSQSPTKPSRKPKLYSSDCHCLSISCSFTRLWPSSHCSTSKNWKPVC 128

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLSALLPFVVA 120
           NGALSTFACST PFIGRVGSR R GNFSLLSFGLNSNAAAEAAGGDSSQLLSALLPFVV 
Sbjct: 129 NGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAEAAGGDSSQLLSALLPFVVL 188

Query: 121 ATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV 180
           ATGIAALA PSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRP+PLSVGFIAQYV
Sbjct: 189 ATGIAALARPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPVPLSVGFIAQYV 248

Query: 181 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTISS 240
           LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTS TTISS
Sbjct: 249 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSITTISS 308

Query: 241 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVA 300
           VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVA
Sbjct: 309 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVA 368

Query: 301 MICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEASRT 360
           MICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHT+AFTLGYWASKIPIVRQEEEASRT
Sbjct: 369 MICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTLAFTLGYWASKIPIVRQEEEASRT 428

Query: 361 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPSLL 420
           ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGS+IRDLPSLL
Sbjct: 429 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSLL 488

Query: 421 IQKTSSIVSAS 431
           IQK+SSIVSAS
Sbjct: 489 IQKSSSIVSAS 499

BLAST of Lsi02G021650 vs. NCBI nr
Match: XP_004150363.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucumis sativus] >KAE8646275.1 hypothetical protein Csa_016116 [Cucumis sativus])

HSP 1 Score: 740.3 bits (1910), Expect = 9.1e-210
Identity = 400/433 (92.38%), Postives = 415/433 (95.84%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSD-CHCLSISCSFTRLCPSSHCSTSKNWNPV 60
           MSAI+L SSP+PFLS  PIKPSRKPKL+SSD CHCLS+SCS TRL PSSHCSTSKNW P+
Sbjct: 1   MSAISLCSSPLPFLSHLPIKPSRKPKLYSSDYCHCLSVSCSLTRLWPSSHCSTSKNWKPL 60

Query: 61  CNGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSN--AAAEAAGGDSSQLLSALLPF 120
           CNGALSTFACST PFIGRVGSR R GNFSLLSFGLNSN  AAAE AGGDSSQLLSALLPF
Sbjct: 61  CNGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAAEGAGGDSSQLLSALLPF 120

Query: 121 VVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIA 180
           VVAATGI+AL+WPSTF WVPKDLYAPALGGIMLSIGIKLSINDFALA+KRP+PLSVGF+A
Sbjct: 121 VVAATGISALSWPSTFNWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSVGFVA 180

Query: 181 QYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTT 240
           QYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTT
Sbjct: 181 QYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTT 240

Query: 241 ISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMP 300
           ISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVA+LQPVMP
Sbjct: 241 ISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAILQPVMP 300

Query: 301 FVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEA 360
           FVAM+CTSLCIGSPLAIN+SQILSIDGARLVLPVL FHTMAFTLGYWASKIPIVRQEEEA
Sbjct: 301 FVAMVCTSLCIGSPLAINKSQILSIDGARLVLPVLVFHTMAFTLGYWASKIPIVRQEEEA 360

Query: 361 SRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLP 420
           SRTISLCTGMQSSTLAGLLATQFLGST AVPPACSVV MAIMGLCLASFWGSGS+IRDLP
Sbjct: 361 SRTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVVMAIMGLCLASFWGSGSKIRDLP 420

Query: 421 SLLIQKTSSIVSA 430
           SLL+QKTSSI SA
Sbjct: 421 SLLVQKTSSIASA 433

BLAST of Lsi02G021650 vs. NCBI nr
Match: KAA0042001.1 (putative sodium/metabolite cotransporter BASS3 [Cucumis melo var. makuwa] >TYK17937.1 putative sodium/metabolite cotransporter BASS3 [Cucumis melo var. makuwa])

HSP 1 Score: 727.2 bits (1876), Expect = 8.0e-206
Identity = 394/433 (90.99%), Postives = 408/433 (94.23%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MS I L SSPVPFLSQSPIKPSRKPK  SSDCHCLSISC+FTRL PSSHCSTSKN  PVC
Sbjct: 1   MSPIPLCSSPVPFLSQSPIKPSRKPKFFSSDCHCLSISCTFTRLWPSSHCSTSKNLKPVC 60

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAA--EAAGGDSSQLLSALLPFV 120
           NGALSTFACST PFIGRVGSR R GNFSLLSFGLNSNAAA  E AGGDSSQ  S LLPFV
Sbjct: 61  NGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAEEGAGGDSSQFFSGLLPFV 120

Query: 121 VAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQ 180
           VAATGI+AL  PSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALA+KRP+PLS+GF+AQ
Sbjct: 121 VAATGISALTCPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSIGFVAQ 180

Query: 181 YVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240
           YVLKPALGVLIARAFGISP+FYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI
Sbjct: 181 YVLKPALGVLIARAFGISPLFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240

Query: 241 SSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPF 300
           SSVLLTPLLTGILIGSVVPVDA AMSKSILQVVLLPVALGLVLNSYAKPIVA++QPVMPF
Sbjct: 241 SSVLLTPLLTGILIGSVVPVDAAAMSKSILQVVLLPVALGLVLNSYAKPIVAIIQPVMPF 300

Query: 301 VAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEAS 360
           VA+ICTSLCIGSPLAINRSQILSI GA+LVLPVLAFH MAFTLGYWASKIPI+RQEEEAS
Sbjct: 301 VAIICTSLCIGSPLAINRSQILSIHGAQLVLPVLAFHAMAFTLGYWASKIPIIRQEEEAS 360

Query: 361 RTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPS 420
           RTISLCTGMQSSTLAGLLATQFLGST AVPPACSVVAMAIMGLCLASFWGSGS+IRDLPS
Sbjct: 361 RTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPS 420

Query: 421 LLIQKTSSIVSAS 431
           LL+QKTSSI  AS
Sbjct: 421 LLVQKTSSIAGAS 433

BLAST of Lsi02G021650 vs. NCBI nr
Match: XP_008447226.1 (PREDICTED: probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucumis melo])

HSP 1 Score: 726.9 bits (1875), Expect = 1.0e-205
Identity = 394/433 (90.99%), Postives = 408/433 (94.23%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MSAI L SSPVPFLSQSPIKPSRKPK  SSDCHCLSISC+FTRL P SHCSTSKN  PVC
Sbjct: 1   MSAIPLCSSPVPFLSQSPIKPSRKPKFFSSDCHCLSISCTFTRLWPFSHCSTSKNLKPVC 60

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAA--EAAGGDSSQLLSALLPFV 120
           NGALSTFACST PFIGRVGSR R GNFSLLSFGLNSNAAA  E AGGDSSQ  S LLPFV
Sbjct: 61  NGALSTFACSTSPFIGRVGSRIRLGNFSLLSFGLNSNAAAAEEGAGGDSSQFFSGLLPFV 120

Query: 121 VAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQ 180
           VAATGI+AL  PSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALA+KRP+PLS+GF+AQ
Sbjct: 121 VAATGISALTCPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAMKRPVPLSIGFVAQ 180

Query: 181 YVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240
           YVLKPALGVLIARAFGISP+FYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI
Sbjct: 181 YVLKPALGVLIARAFGISPLFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTI 240

Query: 241 SSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPF 300
           SSVLLTPLLTGILIGSVVPVDA AMSKSILQVVLLPVALGLVLNSYAKPIVA++QPVMPF
Sbjct: 241 SSVLLTPLLTGILIGSVVPVDAAAMSKSILQVVLLPVALGLVLNSYAKPIVAIIQPVMPF 300

Query: 301 VAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEAS 360
           VA+ICTSLCIGSPLAINRSQILSI GA+LVLPVLAFH MAFTLGYWASKIPI+RQEEEAS
Sbjct: 301 VAIICTSLCIGSPLAINRSQILSIHGAQLVLPVLAFHAMAFTLGYWASKIPIIRQEEEAS 360

Query: 361 RTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPS 420
           RTISLCTGMQSSTLAGLLATQFLGST AVPPACSVVAMAIMGLCLASFWGSGS+IRDLPS
Sbjct: 361 RTISLCTGMQSSTLAGLLATQFLGSTHAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPS 420

Query: 421 LLIQKTSSIVSAS 431
           LL+QKTSSI  AS
Sbjct: 421 LLVQKTSSIAGAS 433

BLAST of Lsi02G021650 vs. NCBI nr
Match: XP_022991663.1 (probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 718.0 bits (1852), Expect = 4.9e-203
Identity = 394/431 (91.42%), Postives = 408/431 (94.66%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSFTRLCPSSHCSTSKNWNPVC 60
           MSAIAL SSPVPF SQ PIKP RKPKL+SSDC CLS SC+ TRL  SS+CSTSKNW+ V 
Sbjct: 1   MSAIALCSSPVPFPSQLPIKPRRKPKLYSSDCLCLSFSCNLTRLW-SSYCSTSKNWSTVR 60

Query: 61  NGALSTFACST-PFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLSALLPFVVA 120
           NGA+STFACST PF+GR+GSR RQGNFSLLSFGLNSNA AEA  GDSSQLLSALLPFVVA
Sbjct: 61  NGAVSTFACSTSPFVGRIGSRIRQGNFSLLSFGLNSNAEAEATDGDSSQLLSALLPFVVA 120

Query: 121 ATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV 180
           AT IAAL  PSTFTWV KD YAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV
Sbjct: 121 ATAIAALTRPSTFTWVSKDFYAPALGGIMLSIGIKLSINDFALAIKRPLPLSVGFIAQYV 180

Query: 181 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILLTSFTTISS 240
           LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALS+LLTSFTTISS
Sbjct: 181 LKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSVLLTSFTTISS 240

Query: 241 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVA 300
           VLLTPLLTGILIGSVVPVDAVAMSKSILQVVL+P++LGLVLNSYAKPIVA+LQPVMPFVA
Sbjct: 241 VLLTPLLTGILIGSVVPVDAVAMSKSILQVVLVPISLGLVLNSYAKPIVALLQPVMPFVA 300

Query: 301 MICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVRQEEEASRT 360
           MICTSLCIGSPLAINRSQILSIDGARLV PVL FHTMAFTLGYWASKIPIVRQEEEASRT
Sbjct: 301 MICTSLCIGSPLAINRSQILSIDGARLVFPVLVFHTMAFTLGYWASKIPIVRQEEEASRT 360

Query: 361 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSRIRDLPSLL 420
           ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGS+IRDLPSLL
Sbjct: 361 ISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSKIRDLPSLL 420

Query: 421 IQKTSSIVSAS 431
           +QKTSSIVSAS
Sbjct: 421 MQKTSSIVSAS 430

BLAST of Lsi02G021650 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 491.1 bits (1263), Expect = 9.0e-139
Identity = 280/439 (63.78%), Postives = 337/439 (76.77%), Query Frame = 0

Query: 1   MSAIALFSSPVPFLSQSPIKPSRKPKLHSSDCHCLSISCSF---TRLCPSSHCSTSKNW- 60
           M+ IA  S P P + +  +      + H+      S +C F   +    S H S+  N+ 
Sbjct: 1   MTLIASLSLPPPAIQRQSLS-----EFHNLPAKSQSFNCQFRSSSSPLSSLHSSSLSNFL 60

Query: 61  ----NPVCNGALSTFACS-TPFIGRVGSRFRQGNFSLLSFGLNSNAAAEAAGGDSSQLLS 120
                   +G +   ACS TPF+GRVG  +R GN SLLSF   ++   +A   DSSQ  S
Sbjct: 61  DFRLRRRNSGLVPVVACSTTPFMGRVGLHWRDGNMSLLSFCGGTDVTEKA---DSSQFWS 120

Query: 121 ALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLS 180
           ALLPFVVA T +AAL++P +FTWV KDLYAPALGGIMLSIGI+LS++DFALA KRP+PLS
Sbjct: 121 ALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVPLS 180

Query: 181 VGFIAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIALSILL 240
           VGF+AQYVLKP LGVL+A AFG+   FYAGF+LT CV GAQLSSYAS LSK D+A+SILL
Sbjct: 181 VGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVAMSILL 240

Query: 241 TSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPIVAVL 300
           TS TTI+SV+ TPLL+G+LIGSVVPVDAVAMSKSILQVVL+P+ LGLVLN+YAKP+V +L
Sbjct: 241 TSSTTIASVIFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKPVVTLL 300

Query: 301 QPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKIPIVR 360
           QPVMPFVAM+CTSLCIGSPL+INRSQILS +G  L++P++ FH +AF LGYW SKIP +R
Sbjct: 301 QPVMPFVAMVCTSLCIGSPLSINRSQILSAEGLGLIVPIVTFHAVAFALGYWFSKIPGLR 360

Query: 361 QEEEASRTISLCTGMQSSTLAGLLATQFLGSTQAVPPACSVVAMAIMGLCLASFWGSGSR 420
           QEEE SRTISLCTGMQSSTLAGLLA+QFLGS+QAVP ACSVV MAIMGLCLASFWG+G R
Sbjct: 361 QEEEVSRTISLCTGMQSSTLAGLLASQFLGSSQAVPAACSVVVMAIMGLCLASFWGNGFR 420

Query: 421 IRDLPSLLIQKTSSIVSAS 431
           IRD+ SL   +++   + S
Sbjct: 421 IRDVLSLSTPQSTGYTAES 431

BLAST of Lsi02G021650 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 204.1 bits (518), Expect = 2.2e-52
Identity = 124/300 (41.33%), Postives = 180/300 (60.00%), Query Frame = 0

Query: 108 QLLSALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRP 167
           +LL+ L P  V    +  +  PS  TW+  DL+   LG +MLS+G+ L+  DF   ++ P
Sbjct: 99  ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLFTLGLGFLMLSMGLTLTFEDFRRCLRNP 158

Query: 168 LPLSVGFIAQYVLKPALGVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDIAL 227
             + VGF+AQY++KP LG LIA    +S     G +L +C  G Q S+ A+++SKG++AL
Sbjct: 159 WTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKGNVAL 218

Query: 228 SILLTSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKPI 287
           S+L+T+ +TI ++++TPLLT +L G +VPVDA  ++ S  QVVL+P  +G++ N +    
Sbjct: 219 SVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKF 278

Query: 288 VAVLQPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGYWASKI 347
            + +  V P + +I T+L   SP+    + +L   GA+L+LPV   H  AF +GYW SK 
Sbjct: 279 TSKIITVTPLIGVILTTLLCASPIG-QVADVLKTQGAQLILPVALLHAAAFAIGYWISKF 338

Query: 348 PIVRQEEEASRTISLCTGMQSSTLAGLLATQ-FLGSTQAVPPACSVVAMAIMGLCLASFW 407
                 E  SRTIS+  GMQSS L  LLA + F     AVP A SVV MA+ G  LA FW
Sbjct: 339 SF---GESTSRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFW 394

BLAST of Lsi02G021650 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 201.1 bits (510), Expect = 1.9e-51
Identity = 127/311 (40.84%), Postives = 192/311 (61.74%), Query Frame = 0

Query: 105 DSSQLLSALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAI 164
           D  +  +++LP VV A+ I AL +P +FTW     + PALG +M ++GI  +  DF  A 
Sbjct: 92  DRMKKANSILPHVVLASTILALIYPPSFTWFTSRYFVPALGFLMFAVGINSNEKDFLEAF 151

Query: 165 KRPLPLSVGFIAQYVLKPALGVLIARA----FGISPMFYAGFLLTACVGGAQLSSYASFL 224
           KRP  + +G++ QY++KP LG +   A    F +     AG +L +CV GAQLS+YA+FL
Sbjct: 152 KRPKAILLGYVGQYLVKPVLGFIFGLAAVSLFQLPTPIGAGIMLVSCVSGAQLSNYATFL 211

Query: 225 SKGDIA-LSILLTSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLV 284
           +   +A LSI++TS +T ++VL+TP+L+ +LIG  +PVD   M  SILQVV+ P+A GL+
Sbjct: 212 TDPALAPLSIVMTSLSTATAVLVTPMLSLLLIGKKLPVDVKGMISSILQVVIAPIAAGLL 271

Query: 285 LNSYAKPIVAVLQPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFT 344
           LN     +   ++P +P ++++ T+ C+G+PLA+N + ++S  GA ++L V  FH  AF 
Sbjct: 272 LNKLFPKVSNAIRPFLPILSVLDTACCVGAPLALNINSVMSPFGATILLLVTMFHLSAFL 331

Query: 345 LGYWASKIPIVRQEEEA---SRTISLCTGMQSSTLAGLLATQFL-GSTQAVPPACSVVAM 404
            GY+ +   + R   +A    RT+S  TGMQSS LA  LAT+F       +PPA S V M
Sbjct: 332 AGYFLTG-SVFRNAPDAKAMQRTLSYETGMQSSLLALALATKFFQDPLVGIPPAISTVVM 391

Query: 405 AIMGLCLASFW 407
           ++MG  L   W
Sbjct: 392 SLMGFTLVLIW 401

BLAST of Lsi02G021650 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-49
Identity = 128/306 (41.83%), Postives = 184/306 (60.13%), Query Frame = 0

Query: 112 ALLPFVVAATGIAALAWPSTFTWVPKDLYAPALGGIMLSIGIKLSINDFALAIKRPLPLS 171
           + +P  +  + I AL +P +FTW     + P LG +M ++GI  +  DF  A+KRP  + 
Sbjct: 100 SFIPHGILLSTILALVYPPSFTWFKPRYFVPGLGFMMFAVGINSNERDFLEALKRPDAIF 159

Query: 172 VGFIAQYVLKPAL----GVLIARAFGISPMFYAGFLLTACVGGAQLSSYASFLSKGDI-A 231
            G+I QY++KP L    GV+    F +     AG +L +CV GAQLS+Y +FL+   + A
Sbjct: 160 AGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVSGAQLSNYTTFLTDPSLAA 219

Query: 232 LSILLTSFTTISSVLLTPLLTGILIGSVVPVDAVAMSKSILQVVLLPVALGLVLNSYAKP 291
           LSI++TS +T ++VL+TP+L+ +LIG  +PVD   M  SILQVV+ P+A GL+LN     
Sbjct: 220 LSIVMTSISTATAVLVTPMLSLLLIGKKLPVDVFGMISSILQVVITPIAAGLLLNRLFPR 279

Query: 292 IVAVLQPVMPFVAMICTSLCIGSPLAINRSQILSIDGARLVLPVLAFHTMAFTLGY---- 351
           +   ++P +P + +I  S CIG+PLA+N   ILS  GA ++  V+ FH +AF  GY    
Sbjct: 280 LSNAIKPFLPALTVIDMSCCIGAPLALNIDSILSPFGATILFLVITFHLLAFVAGYFFTG 339

Query: 352 -WASKIPIVRQEEEASRTISLCTGMQSSTLAGLLATQFL-GSTQAVPPACSVVAMAIMGL 407
            + SK P V+      RTIS  TGMQSS LA  LAT+F       VPPA S V M++MG+
Sbjct: 340 FFFSKAPDVK---ALQRTISYETGMQSSLLALALATKFFQDPLVGVPPAISTVVMSLMGV 399

BLAST of Lsi02G021650 vs. TAIR 10
Match: AT4G12030.1 (bile acid transporter 5 )

HSP 1 Score: 174.1 bits (440), Expect = 2.4e-43
Identity = 117/271 (43.17%), Postives = 166/271 (61.25%), Query Frame = 0

Query: 147 IMLSIGIKLSINDFALAIKRPLPLSVGFIAQYVLKPAL----GVLIARAFGISPMFYAGF 206
           +M ++GI  +  DF  A+KRP  +  G+I QY++KP L    GV+    F +     AG 
Sbjct: 1   MMFAVGINSNERDFLEALKRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGI 60

Query: 207 LLTACVGGAQLSSYASFLSKGDI-ALSILLTSFTTISSVLLTPLLTGILIGSVVPVDAVA 266
           +L +CV GAQLS+Y +FL+   + ALSI++TS +T ++VL+TP+L+ +LIG  +PVD   
Sbjct: 61  MLVSCVSGAQLSNYTTFLTDPSLAALSIVMTSISTATAVLVTPMLSLLLIGKKLPVDVFG 120

Query: 267 MSKSILQVVLLPVALGLVLNSYAKPIVAVLQPVMPFVAMICTSLCIGSPLAINRSQILSI 326
           M  SILQVV+ P+A GL+LN     +   ++P +P + +I  S CIG+PLA+N   ILS 
Sbjct: 121 MISSILQVVITPIAAGLLLNRLFPRLSNAIKPFLPALTVIDMSCCIGAPLALNIDSILSP 180

Query: 327 DGARLVLPVLAFHTMAFTLGY-----WASKIPIVRQEEEASRTISLCTGMQSSTLAGLLA 386
            GA ++  V+ FH +AF  GY     + SK P V+      RTIS  TGMQSS LA  LA
Sbjct: 181 FGATILFLVITFHLLAFVAGYFFTGFFFSKAPDVK---ALQRTISYETGMQSSLLALALA 240

Query: 387 TQFL-GSTQAVPPACSVVAMAIMGLCLASFW 407
           T+F       VPPA S V M++MG+ L + W
Sbjct: 241 TKFFQDPLVGVPPAISTVVMSLMGVSLVTIW 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXE81.3e-13763.78Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Arabidopsis tha... [more]
Q6K7391.3e-12968.00Probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Oryza sativa su... [more]
B8BDK41.8e-5141.35Probable sodium/metabolite cotransporter BASS5, chloroplastic OS=Oryza sativa su... [more]
Q650U01.8e-5141.35Probable sodium/metabolite cotransporter BASS5, chloroplastic OS=Oryza sativa su... [more]
Q1EBV73.1e-5141.33Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A5D3D2223.9e-20690.99Putative sodium/metabolite cotransporter BASS3 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BGX95.1e-20690.99probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucumis melo OX... [more]
A0A6J1JWY12.4e-20391.42probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita maxim... [more]
A0A6J1GQ819.9e-20291.18probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Cucurbita mosch... [more]
A0A6J1DPN96.9e-18786.51probable sodium/metabolite cotransporter BASS3, chloroplastic OS=Momordica chara... [more]
Match NameE-valueIdentityDescription
XP_038899090.15.9e-21796.29probable sodium/metabolite cotransporter BASS3, chloroplastic [Benincasa hispida... [more]
XP_004150363.19.1e-21092.38probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucumis sativus] ... [more]
KAA0042001.18.0e-20690.99putative sodium/metabolite cotransporter BASS3 [Cucumis melo var. makuwa] >TYK17... [more]
XP_008447226.11.0e-20590.99PREDICTED: probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucumi... [more]
XP_022991663.14.9e-20391.42probable sodium/metabolite cotransporter BASS3, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G25410.19.0e-13963.78Sodium Bile acid symporter family [more]
AT2G26900.12.2e-5241.33Sodium Bile acid symporter family [more]
AT4G22840.11.9e-5140.84Sodium Bile acid symporter family [more]
AT4G12030.21.0e-4941.83bile acid transporter 5 [more]
AT4G12030.12.4e-4343.17bile acid transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002657Bile acid:sodium symporter/arsenical resistance protein Acr3PFAMPF01758SBFcoord: 142..320
e-value: 4.6E-31
score: 108.0
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 100..408
e-value: 1.3E-68
score: 233.3
NoneNo IPR availablePANTHERPTHR10361:SF33SODIUM/METABOLITE COTRANSPORTER BASS3, CHLOROPLASTIC-RELATEDcoord: 24..428
IPR004710Bile acid:sodium symporterPANTHERPTHR10361SODIUM-BILE ACID COTRANSPORTERcoord: 24..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G021650.1Lsi02G021650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane