Lsi02G021300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G021300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationchr02: 27534594 .. 27535982 (-)
RNA-Seq ExpressionLsi02G021300
SyntenyLsi02G021300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGAGAAGGGTGAGGTTGGAGGAGATGAAGAGATGATGATTATGGAGAATAATAATAAGAAGAAGAAGAAGGAGGAGGAGGAGATGGGTGAAGTTGTGATATCCATAGAAGAAATGCTCATGGAACTGCCTGCTATCAATCAAAAAAGGTTCATGTATAAAGTTCCCAAATTGCACCGCAAGATTAATGAAACAGTCTATACTCCTCAAGTCATTTCAATTGGGCCATTCCACCATGGCCAACCAGAATTGATGGCCGCGGAACAGTATAAGCTTCGGGCTCTCAAAACCTATCTTGCTCGTATAAACATGAGAGTTGAGGAGGCCATGATTATGGCTTGCTCATGGGAGGAAAAAGCTCGTAGTTGCTATGCACATCCCATACACATGAACAAACAAGACTTTGCAAAAATGATACTCTTAGATGGTTGTTTCATATTGGAGTTTATGAATTCAATAATCATTCAGAGATTTTGCGGCGAAATTGATGAGGATTGGATTGAATATTTACTCATCAATGGTCTAATGTACGATCTAAAACGTGACTTAACTATGCTTGAAAATCAACTTCCTTTCTTTGTTCTTCAACGTTTGTTTAACCTATTACCATTTCCACAAAACAATGATGAAGATCCAAATATCTACTTTATGAAATGCATGGAAAAGTTGTTTATTGCAACCACGGATCAATATGAGTTTCCTTCTAATCTTATCTCAAGTGAAGACACGGTAAAACACTTGGTTGATTTATTAAGACTCTTCTACTTGCCTTCACCTGAGACAGAGGAGTATAAGAAATTCATTGGAACACAAGGGATGACGTTTATCCATCCTCCAACTATAACTGAGCTCTGTGATGCTGGTGTCAAGGTACAGAAAGCAGAAAAAGCTAAGAGCTTCATGGACTGCAGCTTTAAAAATGGGGTTTTGAAAATTCCACCTTTCATAATTCATTCTAACTTTGAAATACACATGCGAAACTTGATACTTTCTGAGATTTTCAGGCCAGGAACAACGGATCACATGTACGTATTCCATTATATTGGATTGTTAGATGGGTTGATTAACACAGAGAAAGACGTGAGTATACTTGTGAAGGAGAATATCATAACCAACGATATTGGTGGCAGTGACCAACAAGTTTCAGAATTAATTAACAATCTACGTCTAAATGCCCCAACTTTTCCTCCTAATTTTTACTTCAACAATATGAGCAGAGATTTAAATGACTACTGCAACAACTGGTGGCATCGATCTATAGCTTTATTAAGGCGTGACTATTTCAATACTCCATGGGCTTCCATCTCCTTCTTTGCTGCTACCTTCCTCCTTATCCTCACTTTACTTCAAACCTTATTCTCTACTCCATTAATTTTTCATCAGTAA

mRNA sequence

ATGGATGAGAAGGGTGAGGTTGGAGGAGATGAAGAGATGATGATTATGGAGAATAATAATAAGAAGAAGAAGAAGGAGGAGGAGGAGATGGGTGAAGTTGTGATATCCATAGAAGAAATGCTCATGGAACTGCCTGCTATCAATCAAAAAAGGTTCATGTATAAAGTTCCCAAATTGCACCGCAAGATTAATGAAACAGTCTATACTCCTCAAGTCATTTCAATTGGGCCATTCCACCATGGCCAACCAGAATTGATGGCCGCGGAACAGTATAAGCTTCGGGCTCTCAAAACCTATCTTGCTCGTATAAACATGAGAGTTGAGGAGGCCATGATTATGGCTTGCTCATGGGAGGAAAAAGCTCGTAGTTGCTATGCACATCCCATACACATGAACAAACAAGACTTTGCAAAAATGATACTCTTAGATGGTTGTTTCATATTGGAGTTTATGAATTCAATAATCATTCAGAGATTTTGCGGCGAAATTGATGAGGATTGGATTGAATATTTACTCATCAATGGTCTAATGTACGATCTAAAACGTGACTTAACTATGCTTGAAAATCAACTTCCTTTCTTTGTTCTTCAACGTTTGTTTAACCTATTACCATTTCCACAAAACAATGATGAAGATCCAAATATCTACTTTATGAAATGCATGGAAAAGTTGTTTATTGCAACCACGGATCAATATGAGTTTCCTTCTAATCTTATCTCAAGTGAAGACACGGTAAAACACTTGGTTGATTTATTAAGACTCTTCTACTTGCCTTCACCTGAGACAGAGGAGTATAAGAAATTCATTGGAACACAAGGGATGACGTTTATCCATCCTCCAACTATAACTGAGCTCTGTGATGCTGGTGTCAAGGTACAGAAAGCAGAAAAAGCTAAGAGCTTCATGGACTGCAGCTTTAAAAATGGGGTTTTGAAAATTCCACCTTTCATAATTCATTCTAACTTTGAAATACACATGCGAAACTTGATACTTTCTGAGATTTTCAGGCCAGGAACAACGGATCACATGTACGTATTCCATTATATTGGATTGTTAGATGGGTTGATTAACACAGAGAAAGACGTGAGTATACTTGTGAAGGAGAATATCATAACCAACGATATTGGTGGCAGTGACCAACAAGTTTCAGAATTAATTAACAATCTACGTCTAAATGCCCCAACTTTTCCTCCTAATTTTTACTTCAACAATATGAGCAGAGATTTAAATGACTACTGCAACAACTGGTGGCATCGATCTATAGCTTTATTAAGGCGTGACTATTTCAATACTCCATGGGCTTCCATCTCCTTCTTTGCTGCTACCTTCCTCCTTATCCTCACTTTACTTCAAACCTTATTCTCTACTCCATTAATTTTTCATCAGTAA

Coding sequence (CDS)

ATGGATGAGAAGGGTGAGGTTGGAGGAGATGAAGAGATGATGATTATGGAGAATAATAATAAGAAGAAGAAGAAGGAGGAGGAGGAGATGGGTGAAGTTGTGATATCCATAGAAGAAATGCTCATGGAACTGCCTGCTATCAATCAAAAAAGGTTCATGTATAAAGTTCCCAAATTGCACCGCAAGATTAATGAAACAGTCTATACTCCTCAAGTCATTTCAATTGGGCCATTCCACCATGGCCAACCAGAATTGATGGCCGCGGAACAGTATAAGCTTCGGGCTCTCAAAACCTATCTTGCTCGTATAAACATGAGAGTTGAGGAGGCCATGATTATGGCTTGCTCATGGGAGGAAAAAGCTCGTAGTTGCTATGCACATCCCATACACATGAACAAACAAGACTTTGCAAAAATGATACTCTTAGATGGTTGTTTCATATTGGAGTTTATGAATTCAATAATCATTCAGAGATTTTGCGGCGAAATTGATGAGGATTGGATTGAATATTTACTCATCAATGGTCTAATGTACGATCTAAAACGTGACTTAACTATGCTTGAAAATCAACTTCCTTTCTTTGTTCTTCAACGTTTGTTTAACCTATTACCATTTCCACAAAACAATGATGAAGATCCAAATATCTACTTTATGAAATGCATGGAAAAGTTGTTTATTGCAACCACGGATCAATATGAGTTTCCTTCTAATCTTATCTCAAGTGAAGACACGGTAAAACACTTGGTTGATTTATTAAGACTCTTCTACTTGCCTTCACCTGAGACAGAGGAGTATAAGAAATTCATTGGAACACAAGGGATGACGTTTATCCATCCTCCAACTATAACTGAGCTCTGTGATGCTGGTGTCAAGGTACAGAAAGCAGAAAAAGCTAAGAGCTTCATGGACTGCAGCTTTAAAAATGGGGTTTTGAAAATTCCACCTTTCATAATTCATTCTAACTTTGAAATACACATGCGAAACTTGATACTTTCTGAGATTTTCAGGCCAGGAACAACGGATCACATGTACGTATTCCATTATATTGGATTGTTAGATGGGTTGATTAACACAGAGAAAGACGTGAGTATACTTGTGAAGGAGAATATCATAACCAACGATATTGGTGGCAGTGACCAACAAGTTTCAGAATTAATTAACAATCTACGTCTAAATGCCCCAACTTTTCCTCCTAATTTTTACTTCAACAATATGAGCAGAGATTTAAATGACTACTGCAACAACTGGTGGCATCGATCTATAGCTTTATTAAGGCGTGACTATTTCAATACTCCATGGGCTTCCATCTCCTTCTTTGCTGCTACCTTCCTCCTTATCCTCACTTTACTTCAAACCTTATTCTCTACTCCATTAATTTTTCATCAGTAA

Protein sequence

MDEKGEVGGDEEMMIMENNNKKKKKEEEEMGEVVISIEEMLMELPAINQKRFMYKVPKLHRKINETVYTPQVISIGPFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQDFAKMILLDGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFFVLQRLFNLLPFPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLISSEDTVKHLVDLLRLFYLPSPETEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLRRDYFNTPWASISFFAATFLLILTLLQTLFSTPLIFHQ
Homology
BLAST of Lsi02G021300 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.1e-30
Identity = 117/447 (26.17%), Postives = 199/447 (44.52%), Query Frame = 0

Query: 36  SIEEMLMELPAINQKRFMYKVPKLHRKINETVYTPQVISIGPFHHGQPELMAAEQYKLRA 95
           S E +L+   A  +   +++VP+    +N   Y P+V+SIGP+H+G+  L   +Q+K R 
Sbjct: 31  SKEPVLLLESAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRL 90

Query: 96  LKTYLARINMR-VEEAMIM--ACSWEEKARSCYAHPIHMNKQDFAKMILLDGCFILEFMN 155
           L+ +L     + VEE +++      E+K R  Y+  +     D   M++LDGCFIL    
Sbjct: 91  LQLFLDEAKKKDVEENVLVKAVVDLEDKIRKSYSEELKTG-HDLMFMMVLDGCFIL---- 150

Query: 156 SIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFFVLQRLFNLLPFPQNNDED 215
            ++     G I+        I  L+  ++ DL +LENQ+PFFVLQ L+       ++D  
Sbjct: 151 -MVFLIMSGNIELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSD-- 210

Query: 216 PNIYFMKCMEKLFIATTDQYEFPSNLISSEDT---------VKHLVDLLRLFYLPSPETE 275
                              + F  N I  E +          KHL+DL+R  +LP+  T 
Sbjct: 211 --------------LNRIAFHFFKNPIDKEGSYWEKHRNYKAKHLLDLIRETFLPN--TS 270

Query: 276 EYKKFIGTQGMTFIHP------PTI-----------TELCDAGVKVQ-KAEKAKSFMDCS 335
           E  K         +H       P++             L   G+K + +  K  S ++  
Sbjct: 271 ESDKASSPHVQVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVR 330

Query: 336 FKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSIL 395
            K   L+IP             N +  E F   +++   +  YI  +  L+N E+DV+ L
Sbjct: 331 LKKNKLQIPQLRFDGFISSFFLNCVAFEQFYTDSSNE--ITTYIVFMGCLLNNEEDVTFL 390

Query: 396 VKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLR 453
             + +I  +  GS+ +VSE    +  +        Y NN+ + +N+Y   W++   A  R
Sbjct: 391 RNDKLIIENHFGSNNEVSEFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFR 450

BLAST of Lsi02G021300 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 8.8e-12
Identity = 60/243 (24.69%), Postives = 106/243 (43.62%), Query Frame = 0

Query: 26  EEEEMGEVVISIEEMLMELPAINQKRFMYKVPKLHRKINETVYTPQVISIGPFHHGQPEL 85
           EE ++ EV +SI                + VPK     +   YTP  +SIGP+H  +PEL
Sbjct: 34  EEHDLEEVTVSI----------------FNVPKALMCSHPDSYTPHRVSIGPYHCLKPEL 93

Query: 86  MAAEQYKLRALKTYLARIN-MRVEEAMIMACSWEEKARSCYAHPIHMNKQDFAKMILLDG 145
              E+YKL   +    + N  R  + +    S E K R+CY   I  N +    ++ +D 
Sbjct: 94  HEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDS 153

Query: 146 CFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFFVLQRLFNLLP 205
            F++EF+     ++         +E L+      ++ RD+ M+ENQ+P FVL++      
Sbjct: 154 SFLIEFLKIYSFRK---------VETLINRVGHNEILRDIMMIENQIPLFVLRKTLEFQL 213

Query: 206 FPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLISSEDTVKHLVDLLRLFYLPSPETEE 265
               + +D  +  +  + K       +++    L +      H++D L    +P  E EE
Sbjct: 214 ESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIVPRIEEEE 251

Query: 266 YKK 268
            ++
Sbjct: 274 LEE 251

BLAST of Lsi02G021300 vs. ExPASy TrEMBL
Match: A0A0A0K6U9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G363030 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.8e-145
Identity = 271/460 (58.91%), Postives = 349/460 (75.87%), Query Frame = 0

Query: 6   EVGGDEEMMIMENNNKKKKKEEEEMG--EVVISIEEMLMELPAINQKRFMYKVPKLHRKI 65
           E+ G ++  +MEN  K   +++EE    +VV+SIE+M M+LP IN K F+Y+VP+LHRK+
Sbjct: 6   EIEGHDDQEMMENYKKAGFEDQEENNDDDVVVSIEKMFMKLPPINPKCFIYQVPELHRKM 65

Query: 66  NETVYTPQVISIGPFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARS 125
           N+  YTPQ+ISIGP HHGQ +  AAEQYK+ ALK YL+RINM V EAM +A  WEE AR+
Sbjct: 66  NDIAYTPQLISIGPLHHGQQQYKAAEQYKVYALKRYLSRINMTVGEAMDIAGRWEETARN 125

Query: 126 CYAHPIHMNKQDFAKMILLDGCFILEFMNSIIIQ-RFCGEIDEDWIEYLLINGLMYDLKR 185
           CYA+PI MN+++F KM+LLD CFILEFMNS + + R+ G+ ++ W+   L+ GL++ LKR
Sbjct: 126 CYANPIDMNREEFVKMMLLDSCFILEFMNSTVCRLRYWGQNEDHWMNDTLLCGLLFGLKR 185

Query: 186 DLTMLENQLPFFVLQRLFNLLPFPQNNDEDPN-IYFMKCMEKLFIATTDQYEFPSNLISS 245
           DL+M ENQLPFF LQ LF L    QN    P+ I F+  M  LFIATT  Y+   N++  
Sbjct: 186 DLSMFENQLPFFPLQELFYLSFNRQNVTNPPSPIIFLTSMAHLFIATTGGYQLRCNVLDD 245

Query: 246 EDTVKHLVDLLRLFYLPSPETEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSF 305
              V+HLVDLLR +Y+PSP+TEEYK +   +  ++I+ PTI+ELC+AGV++Q+A+ AKS 
Sbjct: 246 THKVRHLVDLLRFYYIPSPDTEEYKSY-EAEKSSYINTPTISELCEAGVQIQRADDAKSL 305

Query: 306 MDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKD 365
           +D +FK+GVLKIPPF IH  FEI +RNLI+SEIF   T D  ++F YI LLD LINTEKD
Sbjct: 306 LDFNFKDGVLKIPPFNIHIEFEIQIRNLIVSEIFH-DTDDSKFIFDYIALLDDLINTEKD 365

Query: 366 VSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSI 425
           VSILVKE I+TN+IGGSD+ V +LIN+LRLNAPTFP  +Y++NMS+DLN++C  WW+RS+
Sbjct: 366 VSILVKEKILTNEIGGSDEPVYKLINDLRLNAPTFPLAYYYSNMSKDLNEHCRKWWNRSL 425

Query: 426 ALLRRDYFNTPWASISFFAATFLLILTLLQTLFSTPLIFH 462
           A LRRDYFN PWASISF AAT LLILTLLQTLFS P  FH
Sbjct: 426 ASLRRDYFNNPWASISFVAATVLLILTLLQTLFSAPAFFH 463

BLAST of Lsi02G021300 vs. ExPASy TrEMBL
Match: A0A6J1BVD4 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.1e-100
Identity = 216/441 (48.98%), Postives = 291/441 (65.99%), Query Frame = 0

Query: 24  KKEEEEMGEVVISIEEMLMELPAINQKRF-MYKVPKLHRKINETVYTPQVISIGPFHHGQ 83
           ++ EE+   + +SI ++L E P I    + +Y+VPK    +  T YTP+VI+IGPFHHG+
Sbjct: 41  QQSEEDDLSLELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGR 100

Query: 84  PELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQDFAKMILL 143
            +L+A +Q KL   + YL RI   V+  + +A  WE KAR  YA PI+M   DF +M+L+
Sbjct: 101 NDLLATQQSKLHCFRNYLIRIERDVKYVVAIARQWEIKARRLYAEPINMTSDDFVRMMLI 160

Query: 144 DGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFFVLQRLFNL 203
           D CFI+E M  II+   C E  E + + L    +++DL ++LTMLENQLPFFVLQ LF+L
Sbjct: 161 DACFIVELM--IILNFECFETREGF-DLLFAKVILHDLYQELTMLENQLPFFVLQALFDL 220

Query: 204 LPFPQNNDEDPNIYFMKCMEK-LFIATTDQYEFPSNLISSEDTVKHLVDLLRLFYLPSPE 263
            P P  N    NI F++   K L       Y  P   +SS + V HLVDLL  +Y+PSP+
Sbjct: 221 FP-PHKN--KANISFIQLTGKFLSNGLIRGYNLPCG-VSSTEEVNHLVDLLGFYYVPSPD 280

Query: 264 TEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCSFKNGVLKIPPFIIHSN 323
           TEEY++    +   F+ PPTIT+LC+AGVKV+KA  A+S +D SFK GVL+IPPF IH +
Sbjct: 281 TEEYRQNKEIKNRIFLLPPTITKLCEAGVKVKKAIGARSLLDISFKRGVLQIPPFEIHDD 340

Query: 324 FEIHMRNLILSEIF-RPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENIITNDIGGSDQ 383
           FEI++RNL+  E +      D  YV HYI  LDGLI+T +DV++LVKE II N IGGS++
Sbjct: 341 FEIYVRNLMAFEQYCVREPDDDRYVIHYIEFLDGLISTAEDVALLVKEGIIINHIGGSNK 400

Query: 384 QVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLRRDYFNTPWASISFFA 443
           +VSEL NNL  N P  P  FYF   S++L+D+C  WW RS A LRRDYF++PWA IS FA
Sbjct: 401 EVSELFNNLCKNTP-IPFQFYFYATSKNLHDHCRKWWPRSKATLRRDYFHSPWAYISVFA 460

Query: 444 ATFLLILTLLQTLFSTPLIFH 462
           ATFL++L LLQT+F+    F+
Sbjct: 461 ATFLILLALLQTIFTAKSTFN 473

BLAST of Lsi02G021300 vs. ExPASy TrEMBL
Match: A0A6J1BQT6 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004764 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 1.9e-89
Identity = 195/441 (44.22%), Postives = 273/441 (61.90%), Query Frame = 0

Query: 18  NNNKKKKKEEEEMGEVVISIEEMLMEL--PAINQKRFMYKVPKLHRKINETVYTPQVISI 77
           NNN   + +E+    VVISIEEM   L  P I+ +  +Y+VPK    +N   YTPQVISI
Sbjct: 25  NNN---EVDEQHCRHVVISIEEMCKRLPPPPIDPECSIYRVPKRLLNMNRKAYTPQVISI 84

Query: 78  GPFHH-GQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQ 137
           GPFHH  Q  L+  +Q+KL+AL +YL R+ M VE  + +  +WE +ARSCY  PI MN  
Sbjct: 85  GPFHHSNQSNLIVTQQHKLQALDSYLHRVKMTVEAVVKITQNWENRARSCYGEPIKMNND 144

Query: 138 DFAKMILLDGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFF 197
            F  M+LLDGCF++ F   +I+     E DE+  +      +  D+  D+TMLENQLPFF
Sbjct: 145 KFVTMLLLDGCFVVXF---LILDYNNYETDENGFDSSFYEAMSSDIYGDMTMLENQLPFF 204

Query: 198 VLQRLFNLLPFPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLISSEDTVKHLVDLLRL 257
           VLQ L++L+P    + E  N   ++ +E  F  + + +E P ++  S   VKHLVDLL L
Sbjct: 205 VLQGLYDLIP---KDHEIKNNSLIQLIETFFSRSMNNHEIPCHV--SPPNVKHLVDLLSL 264

Query: 258 FYLPSPETEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCSFKNGVLKIP 317
           ++LP  +T++           ++  P +TELC+AGV ++K ++A   MD SFKNGVL+IP
Sbjct: 265 YFLPPCDTKQQH-----DKDEYLVTPCVTELCEAGVTIKKGKEATCLMDISFKNGVLEIP 324

Query: 318 PFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENIITND 377
           P  I  +FE  +RNL+  E + P    H Y   Y   LD +I+TEKDV +LV+  II N 
Sbjct: 325 PLDIDDHFETIVRNLMAFEHY-PAANYHRYTIQYALFLDYMISTEKDVRLLVEAGIIINS 384

Query: 378 IGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLRRDYFNTPWA 437
           IGGSD++VS L N+L           Y N++++ L+D+C  WW RS A L+RDYFN+PWA
Sbjct: 385 IGGSDKEVSRLFNDLGKYVSIPGGVHYLNHITKPLHDHCKKWWPRSKATLKRDYFNSPWA 444

Query: 438 SISFFAATFLLILTLLQTLFS 456
            IS  AAT+++ILTLLQT+F+
Sbjct: 445 FISIVAATYIIILTLLQTIFT 448

BLAST of Lsi02G021300 vs. ExPASy TrEMBL
Match: A0A1S3BBL9 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488293 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 8.1e-85
Identity = 195/444 (43.92%), Postives = 273/444 (61.49%), Query Frame = 0

Query: 33  VVISIEEMLMELPAINQKR-FMYKVPKLHRKINETVYTPQVISIGPFH-HGQPELMAAEQ 92
           VVISIE+ML ++P  ++ +  +Y+VPK  R++N   Y PQ+ISIGPFH H    L+A EQ
Sbjct: 41  VVISIEKMLDQVPPTHENQCSIYRVPKQLREMNPKAYAPQLISIGPFHYHTHKNLIANEQ 100

Query: 93  YKLRALKTYLARI-----------NMRVEEAMIMACSWEEKARSCYAHPIHMNKQDFAKM 152
           YKL+    YL R+              VE+ +  A SW E+AR+CYA  I+MN +DF KM
Sbjct: 101 YKLQGFINYLRRVYKMESLEQLVRTKSVEDLVKRAQSWVEEARNCYAETINMNDEDFIKM 160

Query: 153 ILLDGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMY-------DLKRDLTMLENQLP 212
           +L+DGCFI+EF   I+      E  E     +  N  M        D+  DL  LENQLP
Sbjct: 161 MLVDGCFIVEFF--ILDFEEYNESHESLFPQIENNVSMSFYKERIPDIDEDLIKLENQLP 220

Query: 213 FFVLQRLFNLLPFPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLISSEDTVKHLVDLL 272
           FFVLQ LF+L+P    + + PN +     E L +   + YE PS+++S +   KH +D L
Sbjct: 221 FFVLQHLFDLIP---KHKDAPNCFKQLTYEYLTMGWLENYE-PSDILSIKP--KHFIDFL 280

Query: 273 RLFYLPSPETEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEK-AKSFMDCSFKNGVL 332
             + +P  + E  +K    +    I PP+ITE+C+AGV ++KA+K  K  ++  F+NG+L
Sbjct: 281 SFYLVPEHQYEHDQKSNDEEEWNIIIPPSITEICEAGVTIKKADKNTKCLLNIRFENGIL 340

Query: 333 KIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENII 392
           +IPP  I   FE  MRNL+  E F P    + YV  Y+  +D LI TEKDV++LVKE II
Sbjct: 341 EIPPLHIDDYFEPMMRNLLAFEHF-PVEVKNTYVIPYLTFMDYLIITEKDVNLLVKEKII 400

Query: 393 TNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLRRDYFNT 452
            NDIGGSD++VS+L NNL     + P + YFN+ S+ L D+C+  W+++ A L+ +YFNT
Sbjct: 401 INDIGGSDREVSQLFNNLCKFVSSSPNDNYFNDTSKALRDHCDRRWNKAKASLKHNYFNT 460

Query: 453 PWASISFFAATFLLILTLLQTLFS 456
           PWA+IS  AATFLL+LT+LQT+FS
Sbjct: 461 PWAAISVSAATFLLVLTILQTIFS 475

BLAST of Lsi02G021300 vs. ExPASy TrEMBL
Match: A0A6J1DXD6 (UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 7.6e-83
Identity = 191/450 (42.44%), Postives = 269/450 (59.78%), Query Frame = 0

Query: 13  MMIMENNNKKKKKEEEEMGEVVISIEEMLMELPAINQKRFMYKVPKLHRKINETVYTPQV 72
           ++ ++NN          + EV  SI++ML ELP + ++  +++VP+   K N   Y PQ+
Sbjct: 11  VLAVQNNKPYNNMHMTNVDEVCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQI 70

Query: 73  ISIGPFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMN 132
           ISIGPFHHG+ +LM  EQ+KLR L  YL R N  +E  + +  SWE  AR+CYA PI+M+
Sbjct: 71  ISIGPFHHGRQDLMPMEQHKLRFLDRYLRRTNFGIEVTVGIVRSWETTARNCYAEPINMD 130

Query: 133 KQDFAKMILLDGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLP 192
             +F KM+L+DGCFI+E M  +++ R  G   E   + LL   +M DL  DL MLENQLP
Sbjct: 131 SDEFVKMMLVDGCFIVELM--MMVCRI-GSETETRFDPLLFCAMMTDLYCDLIMLENQLP 190

Query: 193 FFVLQRLFNLLPFPQNNDEDPNIYFMKCMEKLF----IATTDQYEFPSNLISSEDTVKHL 252
           FFVLQ LF+          +  + F++     +    +      E P  ++ S   V HL
Sbjct: 191 FFVLQGLFDQFSL------EAGLSFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHL 250

Query: 253 VDLLRLFYLPSPETEEYKKF---IGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCS 312
           VD L  +Y P+P +  +      I  +  TF  PPT+TEL +AG+  +KA +AK  MD S
Sbjct: 251 VDFLSFYYAPAPASVSFTSHSLAISRKKCTF--PPTVTELWEAGIVFKKAMRAKHIMDIS 310

Query: 313 FKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSIL 372
           FK+ VL+IPP  I   FE ++RNL+  E +     D  Y   Y   L+GLI+ E+DVS+L
Sbjct: 311 FKDRVLQIPPLEIGDVFETYVRNLMAFEQYH---NDGKYAIQYFLFLNGLISREQDVSLL 370

Query: 373 VKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLR 432
           VK NIITN IGG++Q+VS L N+L  +         FN+++  L+++C   W++ +A LR
Sbjct: 371 VKANIITNCIGGNNQEVSTLFNDLCKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLR 430

Query: 433 RDYFNTPWASISFFAATFLLILTLLQTLFS 456
           RDYFNTPWA ISF AA FL++LT LQTLFS
Sbjct: 431 RDYFNTPWAFISFVAAAFLILLTFLQTLFS 446

BLAST of Lsi02G021300 vs. NCBI nr
Match: XP_038899472.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 594.3 bits (1531), Expect = 8.7e-166
Identity = 310/463 (66.95%), Postives = 373/463 (80.56%), Query Frame = 0

Query: 1   MDEKGEVGGDEEMMIMENNNKKKKKEEEEMGEVVISIEEMLMELPAINQKRFMYKVPKLH 60
           M +KGEV GDEE MIMENNN  K  EE +  +VVISIE+M+MELP++N    +YKVPK H
Sbjct: 20  MSKKGEVEGDEE-MIMENNNNNKDVEEGD--DVVISIEKMIMELPSVNPNSSLYKVPKGH 79

Query: 61  RKINETVYTPQVISIGPFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEK 120
            K NET+YTP+ ISIGPFHHGQPELMAAEQYKL ALKTYL R  M V+EAM +ACSWEEK
Sbjct: 80  IKTNETMYTPRFISIGPFHHGQPELMAAEQYKLWALKTYLCRAKMGVKEAMSLACSWEEK 139

Query: 121 ARSCYAHPIHMNKQDFAKMILLDGCFILEFMNSIIIQRFCGEIDEDWI-EYLLINGLMYD 180
           ARSCY HPI MNKQ+FAKM+LLDGCFILEF+N  I      E DE WI +  LI  ++YD
Sbjct: 140 ARSCYGHPIDMNKQNFAKMMLLDGCFILEFLN--ITNDLYIEDDEKWIDDGSLIYRILYD 199

Query: 181 LKRDLTMLENQLPFFVLQRLFNLLPFPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLI 240
           ++RDLT+ ENQLPFFVLQ+L++LLPFP  ND DP ++FM+CM KLFIATT  Y +  N  
Sbjct: 200 VERDLTIYENQLPFFVLQQLYDLLPFPPEND-DPAVHFMECMSKLFIATTTGYSYHYNAT 259

Query: 241 SSEDTVKHLVDLLRLFYLPSPETEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAK 300
           +    V+HLVDLL+ +++PSP+T+EYKKF  ++  +++  P +T+LC+AGVKVQKA++AK
Sbjct: 260 TE---VRHLVDLLKFYFIPSPDTDEYKKFKESEEESYLESPNLTKLCEAGVKVQKADEAK 319

Query: 301 SFMDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTE 360
           S MD SFKNG+LKIPP IIH+ FEIH+RNLI+SEI    + D  Y+FHY+ LLD LI+TE
Sbjct: 320 SMMDFSFKNGILKIPPTIIHNVFEIHIRNLIVSEIV---SEDETYLFHYVALLDDLIDTE 379

Query: 361 KDVSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPN-FYFNNMSRDLNDYCNNWWH 420
           KDVSILVKE II+N  G SDQQV+ELINNLRLNAPTFP   +Y+N+MS++LNDYCN WWH
Sbjct: 380 KDVSILVKEKIISNTSGCSDQQVAELINNLRLNAPTFPDKCYYYNHMSKELNDYCNKWWH 439

Query: 421 RSIALLRRDYFNTPWASISFFAATFLLILTLLQTLFSTPLIFH 462
           RS+A LRRDYFNTPWASISFFAATFLLILT LQTLFSTP IF+
Sbjct: 440 RSMASLRRDYFNTPWASISFFAATFLLILTFLQTLFSTPFIFY 470

BLAST of Lsi02G021300 vs. NCBI nr
Match: XP_004149334.2 (UPF0481 protein At3g47200 [Cucumis sativus] >KGN44649.1 hypothetical protein Csa_016145 [Cucumis sativus])

HSP 1 Score: 525.8 bits (1353), Expect = 3.8e-145
Identity = 271/460 (58.91%), Postives = 349/460 (75.87%), Query Frame = 0

Query: 6   EVGGDEEMMIMENNNKKKKKEEEEMG--EVVISIEEMLMELPAINQKRFMYKVPKLHRKI 65
           E+ G ++  +MEN  K   +++EE    +VV+SIE+M M+LP IN K F+Y+VP+LHRK+
Sbjct: 6   EIEGHDDQEMMENYKKAGFEDQEENNDDDVVVSIEKMFMKLPPINPKCFIYQVPELHRKM 65

Query: 66  NETVYTPQVISIGPFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARS 125
           N+  YTPQ+ISIGP HHGQ +  AAEQYK+ ALK YL+RINM V EAM +A  WEE AR+
Sbjct: 66  NDIAYTPQLISIGPLHHGQQQYKAAEQYKVYALKRYLSRINMTVGEAMDIAGRWEETARN 125

Query: 126 CYAHPIHMNKQDFAKMILLDGCFILEFMNSIIIQ-RFCGEIDEDWIEYLLINGLMYDLKR 185
           CYA+PI MN+++F KM+LLD CFILEFMNS + + R+ G+ ++ W+   L+ GL++ LKR
Sbjct: 126 CYANPIDMNREEFVKMMLLDSCFILEFMNSTVCRLRYWGQNEDHWMNDTLLCGLLFGLKR 185

Query: 186 DLTMLENQLPFFVLQRLFNLLPFPQNNDEDPN-IYFMKCMEKLFIATTDQYEFPSNLISS 245
           DL+M ENQLPFF LQ LF L    QN    P+ I F+  M  LFIATT  Y+   N++  
Sbjct: 186 DLSMFENQLPFFPLQELFYLSFNRQNVTNPPSPIIFLTSMAHLFIATTGGYQLRCNVLDD 245

Query: 246 EDTVKHLVDLLRLFYLPSPETEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSF 305
              V+HLVDLLR +Y+PSP+TEEYK +   +  ++I+ PTI+ELC+AGV++Q+A+ AKS 
Sbjct: 246 THKVRHLVDLLRFYYIPSPDTEEYKSY-EAEKSSYINTPTISELCEAGVQIQRADDAKSL 305

Query: 306 MDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKD 365
           +D +FK+GVLKIPPF IH  FEI +RNLI+SEIF   T D  ++F YI LLD LINTEKD
Sbjct: 306 LDFNFKDGVLKIPPFNIHIEFEIQIRNLIVSEIFH-DTDDSKFIFDYIALLDDLINTEKD 365

Query: 366 VSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSI 425
           VSILVKE I+TN+IGGSD+ V +LIN+LRLNAPTFP  +Y++NMS+DLN++C  WW+RS+
Sbjct: 366 VSILVKEKILTNEIGGSDEPVYKLINDLRLNAPTFPLAYYYSNMSKDLNEHCRKWWNRSL 425

Query: 426 ALLRRDYFNTPWASISFFAATFLLILTLLQTLFSTPLIFH 462
           A LRRDYFN PWASISF AAT LLILTLLQTLFS P  FH
Sbjct: 426 ASLRRDYFNNPWASISFVAATVLLILTLLQTLFSAPAFFH 463

BLAST of Lsi02G021300 vs. NCBI nr
Match: XP_038896405.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 512.7 bits (1319), Expect = 3.3e-141
Identity = 270/446 (60.54%), Postives = 330/446 (73.99%), Query Frame = 0

Query: 26  EEEEMGEVVISIEEMLMELPAINQKRFMYKVPKLHRKINETVYTPQVISIGPFHHGQPEL 85
           ++ E   V ISI++ LMELP  N + F+YKVP+L RK+NETVYTPQ ISIGPFHHG+PEL
Sbjct: 2   DKGEGDHVTISIKKTLMELPPTNPESFLYKVPRLRRKMNETVYTPQFISIGPFHHGRPEL 61

Query: 86  MAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQDFAKMILLDGC 145
           MA EQYKLRALKTYLAR+N++VEEAM M  SWEEKARSCY H I MNKQDFAKM+LLDGC
Sbjct: 62  MAVEQYKLRALKTYLARVNLKVEEAMSMTFSWEEKARSCYGHSIDMNKQDFAKMMLLDGC 121

Query: 146 FILEFMNSI-IIQRFCGEID-EDWIEYLLINGLMYDLKRDLTMLENQLPFFVLQRLFNLL 205
           FILEFMNS   +     + D E+WI+ +L+ G+M D++RDL + ENQLPFFVLQ+L+ L 
Sbjct: 122 FILEFMNSTNYMDSLLSKGDHEEWIDGVLLIGVMDDVERDLIIFENQLPFFVLQQLYELS 181

Query: 206 PFPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLISSEDTVKHLVDLLRLFYLPSPETE 265
                  +D  + FM+ M +LFI+TT  Y  P         V+H+VDLLR +Y+PSPET+
Sbjct: 182 FSSTKKSKDLTVNFMEWMAELFISTTSGYALP---FKETTKVRHVVDLLRFYYIPSPETD 241

Query: 266 EYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCSFKNGVLKIPPFIIHSNFE 325
           EY KF   +G+     PTIT+LC+ G+KVQ+A +A S MD SFKNGVLKIPPF IH+NFE
Sbjct: 242 EYNKFNDNEGIYSYQSPTITKLCETGIKVQRAMEADSMMDFSFKNGVLKIPPFRIHTNFE 301

Query: 326 IHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENIITNDIGGSDQQVS 385
           I +RNLI+SEIF        Y+FHY+ LLD LI TEKDVSIL+KE II N+  GS +QV 
Sbjct: 302 IQIRNLIVSEIFNV-VEGKKYIFHYLLLLDDLIKTEKDVSILMKEKIIINETDGSAEQVL 361

Query: 386 ELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLR-------RDYFNTPWASI 445
           +LI+NL L AP++ P  YFNNMSRDL++YC   W RS+A LR       RDYF+TPWA I
Sbjct: 362 KLIHNLHLIAPSYRPQSYFNNMSRDLDNYCEKRWQRSMASLRRSMASLKRDYFSTPWAFI 421

Query: 446 SFFAATFLLILTLLQTLFSTPLIFHQ 463
           SF AATFLLIL+LLQTLFS P IFH+
Sbjct: 422 SFLAATFLLILSLLQTLFSAPSIFHK 443

BLAST of Lsi02G021300 vs. NCBI nr
Match: XP_022132118.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 377.1 bits (967), Expect = 2.2e-100
Identity = 216/441 (48.98%), Postives = 291/441 (65.99%), Query Frame = 0

Query: 24  KKEEEEMGEVVISIEEMLMELPAINQKRF-MYKVPKLHRKINETVYTPQVISIGPFHHGQ 83
           ++ EE+   + +SI ++L E P I    + +Y+VPK    +  T YTP+VI+IGPFHHG+
Sbjct: 41  QQSEEDDLSLELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGR 100

Query: 84  PELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQDFAKMILL 143
            +L+A +Q KL   + YL RI   V+  + +A  WE KAR  YA PI+M   DF +M+L+
Sbjct: 101 NDLLATQQSKLHCFRNYLIRIERDVKYVVAIARQWEIKARRLYAEPINMTSDDFVRMMLI 160

Query: 144 DGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFFVLQRLFNL 203
           D CFI+E M  II+   C E  E + + L    +++DL ++LTMLENQLPFFVLQ LF+L
Sbjct: 161 DACFIVELM--IILNFECFETREGF-DLLFAKVILHDLYQELTMLENQLPFFVLQALFDL 220

Query: 204 LPFPQNNDEDPNIYFMKCMEK-LFIATTDQYEFPSNLISSEDTVKHLVDLLRLFYLPSPE 263
            P P  N    NI F++   K L       Y  P   +SS + V HLVDLL  +Y+PSP+
Sbjct: 221 FP-PHKN--KANISFIQLTGKFLSNGLIRGYNLPCG-VSSTEEVNHLVDLLGFYYVPSPD 280

Query: 264 TEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCSFKNGVLKIPPFIIHSN 323
           TEEY++    +   F+ PPTIT+LC+AGVKV+KA  A+S +D SFK GVL+IPPF IH +
Sbjct: 281 TEEYRQNKEIKNRIFLLPPTITKLCEAGVKVKKAIGARSLLDISFKRGVLQIPPFEIHDD 340

Query: 324 FEIHMRNLILSEIF-RPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENIITNDIGGSDQ 383
           FEI++RNL+  E +      D  YV HYI  LDGLI+T +DV++LVKE II N IGGS++
Sbjct: 341 FEIYVRNLMAFEQYCVREPDDDRYVIHYIEFLDGLISTAEDVALLVKEGIIINHIGGSNK 400

Query: 384 QVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLRRDYFNTPWASISFFA 443
           +VSEL NNL  N P  P  FYF   S++L+D+C  WW RS A LRRDYF++PWA IS FA
Sbjct: 401 EVSELFNNLCKNTP-IPFQFYFYATSKNLHDHCRKWWPRSKATLRRDYFHSPWAYISVFA 460

Query: 444 ATFLLILTLLQTLFSTPLIFH 462
           ATFL++L LLQT+F+    F+
Sbjct: 461 ATFLILLALLQTIFTAKSTFN 473

BLAST of Lsi02G021300 vs. NCBI nr
Match: XP_022131634.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 339.7 bits (870), Expect = 3.9e-89
Identity = 195/441 (44.22%), Postives = 273/441 (61.90%), Query Frame = 0

Query: 18  NNNKKKKKEEEEMGEVVISIEEMLMEL--PAINQKRFMYKVPKLHRKINETVYTPQVISI 77
           NNN   + +E+    VVISIEEM   L  P I+ +  +Y+VPK    +N   YTPQVISI
Sbjct: 25  NNN---EVDEQHCRHVVISIEEMCKRLPPPPIDPECSIYRVPKRLLNMNRKAYTPQVISI 84

Query: 78  GPFHH-GQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQ 137
           GPFHH  Q  L+  +Q+KL+AL +YL R+ M VE  + +  +WE +ARSCY  PI MN  
Sbjct: 85  GPFHHSNQSNLIVTQQHKLQALDSYLHRVKMTVEAVVKITQNWENRARSCYGEPIKMNND 144

Query: 138 DFAKMILLDGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFF 197
            F  M+LLDGCF++ F   +I+     E DE+  +      +  D+  D+TMLENQLPFF
Sbjct: 145 KFVTMLLLDGCFVVXF---LILDYNNYETDENGFDSSFYEAMSSDIYGDMTMLENQLPFF 204

Query: 198 VLQRLFNLLPFPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLISSEDTVKHLVDLLRL 257
           VLQ L++L+P    + E  N   ++ +E  F  + + +E P ++  S   VKHLVDLL L
Sbjct: 205 VLQGLYDLIP---KDHEIKNNSLIQLIETFFSRSMNNHEIPCHV--SPPNVKHLVDLLSL 264

Query: 258 FYLPSPETEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCSFKNGVLKIP 317
           ++LP  +T++           ++  P +TELC+AGV ++K ++A   MD SFKNGVL+IP
Sbjct: 265 YFLPPCDTKQQH-----DKDEYLVTPCVTELCEAGVTIKKGKEATCLMDISFKNGVLEIP 324

Query: 318 PFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENIITND 377
           P  I  +FE  +RNL+  E + P    H Y   Y   LD +I+TEKDV +LV+  II N 
Sbjct: 325 PLDIDDHFETIVRNLMAFEHY-PAANYHRYTIQYALFLDYMISTEKDVRLLVEAGIIINS 384

Query: 378 IGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLRRDYFNTPWA 437
           IGGSD++VS L N+L           Y N++++ L+D+C  WW RS A L+RDYFN+PWA
Sbjct: 385 IGGSDKEVSRLFNDLGKYVSIPGGVHYLNHITKPLHDHCKKWWPRSKATLKRDYFNSPWA 444

Query: 438 SISFFAATFLLILTLLQTLFS 456
            IS  AAT+++ILTLLQT+F+
Sbjct: 445 FISIVAATYIIILTLLQTIFT 448

BLAST of Lsi02G021300 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 229.2 bits (583), Expect = 6.8e-60
Identity = 150/434 (34.56%), Postives = 231/434 (53.23%), Query Frame = 0

Query: 23  KKKEEEEMGEVVISIEEMLMELPAINQKRFMYKVPKLHRKINETVYTPQVISIGPFHHGQ 82
           ++  + E   +V SI+  L  L +++ K  +YKVP   R++N   YTP+++S GP H G+
Sbjct: 265 ERMNQNEGDALVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGK 324

Query: 83  PELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQDFAKMILL 142
            EL A E  K R L +++ R N  +E+ + +A +WE+ ARSCYA  + ++  +F +M+++
Sbjct: 325 EELQAMEDQKYRYLLSFIPRTNSSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVV 384

Query: 143 DGCFILEFMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFFVLQRLFNL 202
           DG F++E +      R  GE D  +   ++I     D+ RD+ ++ENQLPFFV++ +F L
Sbjct: 385 DGSFLVELLLRSHYPRLRGENDRIFGNSMMIT----DVCRDMILIENQLPFFVVKEIFLL 444

Query: 203 LPFPQNNDEDPNIYFMKCMEKLFIATTDQYEFPSNLISSEDTVKHLVDLLRLFYLPS-PE 262
           L           I   +     F++  D  +F +         +H VDLLR  YLP  P 
Sbjct: 445 LLNYYQQGTPSIIQLAQRHFSYFLSRIDDEKFITE-------PEHFVDLLRSCYLPQFPI 504

Query: 263 TEEYKKFIGTQGMTFIHPPTITELCDAGVKVQKAEKAKSFMDCSFKNGVLKIPPFIIHSN 322
             EY        +   + P  TEL  AGV+ + AE +   +D SF +GVLKIP  ++   
Sbjct: 505 KLEYTT------VKVDNAPEATELHTAGVRFKPAETSSCLLDISFADGVLKIPTIVVDDL 564

Query: 323 FEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKDVSILVKENIITNDIGGSDQQ 382
            E   +N+I  E  R    + +    YI LL   I +  D  +L+   II N +G S   
Sbjct: 565 TESLYKNIIGFEQCRCSNKNFL---DYIMLLGCFIKSPTDADLLIHSGIIVNYLGNS-VD 624

Query: 383 VSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSIALLRRDYFNTPWASISFFAA 442
           VS L N++      +   FYF+ +S +L  YCN  W+R  A+LRRDYF+ PWA  S FAA
Sbjct: 625 VSNLFNSISKEV-IYDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAA 676

Query: 443 TFLLILTLLQTLFS 456
             LL+LT +Q++ S
Sbjct: 685 LLLLLLTFIQSVCS 676

BLAST of Lsi02G021300 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 213.4 bits (542), Expect = 3.9e-55
Identity = 159/460 (34.57%), Postives = 236/460 (51.30%), Query Frame = 0

Query: 23  KKKEEEEMGEV-VISIEEMLMEL-----PAINQKRFMYKVPKLHRKINETVYTPQVISIG 82
           +++ EE  G+  VISI + L +        I  K  +Y+VP   ++ ++  Y PQ +S+G
Sbjct: 79  EERPEETTGDSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKKSYFPQTVSLG 138

Query: 83  PFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHMNKQDF 142
           P+HHG+  L   E++K RAL   L R+  R+E         EEKAR+CY  PI +++ +F
Sbjct: 139 PYHHGKKRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKARACYEGPISLSRNEF 198

Query: 143 AKMILLDGCFILE-FMNSIIIQRFCGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPFFV 202
            +M++LDGCF+LE F  ++      G    D +    + GLM+ ++RD+ MLENQLP FV
Sbjct: 199 TEMLVLDGCFVLELFRGTVEGFTEIGYARNDPV--FAMRGLMHSIQRDMIMLENQLPLFV 258

Query: 203 LQRLFNLLPFPQNNDEDPNIYFMKCMEKLF-----IATTDQYEFPSNLISSEDTV----- 262
           L RL  L    QN         +K  + L      +   DQ +  + L  S DT+     
Sbjct: 259 LDRLLELQLGTQNQTGIVAHVAVKFFDPLMPTGEALTKPDQSKLMNWLEKSLDTLGDKGE 318

Query: 263 KHLVDLLRLFYL---PSPETEEYKK-------FIGTQGMTFIHPPTITELCDAGVKVQKA 322
            H +D+ R   L   P+P T    K        +  +    +H   +TEL +AGVK +K 
Sbjct: 319 LHCLDVFRRSLLQSSPTPNTRSLLKRLTRNTRVVDKRQQQLVH--CVTELREAGVKFRK- 378

Query: 323 EKAKSFMDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGL 382
            K   F D  FKNG L+IP  +IH   +    NLI  E     +++H  +  YI  +D L
Sbjct: 379 RKTDRFWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIESSNH--ITSYIIFMDNL 438

Query: 383 INTEKDVSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNN 442
           IN+ +DVS L    II + + GSD +V++L N L       P + + + +S D+N Y N 
Sbjct: 439 INSSEDVSYLHYCGIIEHWL-GSDSEVADLFNRLCQEVVFDPKDSHLSRLSGDVNRYYNR 498

Query: 443 WWHRSIALLRRDYFNTPWASISFFAATFLLILTLLQTLFS 456
            W+   A L   YFN PWA  SF AA  LL+LTL Q+ ++
Sbjct: 499 KWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYA 530

BLAST of Lsi02G021300 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 204.1 bits (518), Expect = 2.4e-52
Identity = 158/454 (34.80%), Postives = 225/454 (49.56%), Query Frame = 0

Query: 22  KKKKEEEEMGEVVISIE---EMLMELPAIN--QKRFMYKVPKLHRKINETVYTPQVISIG 81
           K++K  E   E VISI+   E  +   A N   K  +Y+VP   ++ ++  Y PQ +SIG
Sbjct: 56  KEEKPRETREEWVISIKDKMEKALSYDATNSWDKLCIYRVPFYLQENDKKSYLPQTVSIG 115

Query: 82  PFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAHPIHM-NKQD 141
           P+HHG+  L   E++K RA+   +AR    +E  +      EE+AR+CY  PI M N  +
Sbjct: 116 PYHHGKVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMKELEEEARACYQGPIDMKNSNE 175

Query: 142 FAKMILLDGCFILEFMNSIIIQRF--CGEIDEDWIEYLLINGLMYDLKRDLTMLENQLPF 201
           F +M++LDGCF+LE      IQ F   G    D +      GLM+ ++RD+ MLENQLP 
Sbjct: 176 FTEMLVLDGCFVLELFKG-TIQGFQKIGYARNDPV--FAKRGLMHSIQRDMIMLENQLPL 235

Query: 202 FVLQRLFNLLPFPQNND----EDPNIYFMKCMEKLFIATTDQYEFPSNLISSE---DTVK 261
           FVL RL  L     N      E    +F   M    + T  +    S   S E   +   
Sbjct: 236 FVLDRLLGLQTGTPNQTGIVAEVAVRFFKTLMPTSEVLTKSERSLDSQEKSDELGDNGGL 295

Query: 262 HLVDLLRLFYLPSPETEEYKKFIGTQGMT-----FIHPPTITELCDAGVKVQKAEKAKSF 321
           H +D+     + S ET           M       IH   +TEL  AGV   + E  +  
Sbjct: 296 HCLDVFHRSLIQSSETTNQGTPYEDMSMVEKQQQLIH--CVTELRGAGVNFMRKETGQ-L 355

Query: 322 MDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDGLINTEKD 381
            D  FKNG LKIP  +IH   +    NLI  E     ++++  +  YI  +D LIN+ +D
Sbjct: 356 WDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNN--ITSYIIFMDNLINSSQD 415

Query: 382 VSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCNNWWHRSI 441
           VS L  + II + + GSD +V++L N L       P + Y + +SR++N Y +  W+   
Sbjct: 416 VSYLHHDGIIEHWL-GSDSEVADLFNRLCKEVIFDPKDGYLSQLSREVNRYYSRKWNSLK 475

Query: 442 ALLRRDYFNTPWASISFFAATFLLILTLLQTLFS 456
           A LR+ YFN PWA  SF AA  LL LT  Q+ F+
Sbjct: 476 ATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFA 500

BLAST of Lsi02G021300 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-50
Identity = 157/461 (34.06%), Postives = 235/461 (50.98%), Query Frame = 0

Query: 1   MDEKGEVGGDEEMMIMENNNKKKKKEEEEMGEVVISIEEMLMEL----PAINQKRFMYKV 60
           ++EK      E ++ +E+ N++K +E       VIS+ + +  L            +Y+V
Sbjct: 55  IEEKPRETQVESVVSIEDKNEQKLRE-----IWVISLNDKMKTLGDNATTSWDNLCIYRV 114

Query: 61  PKLHRKINETVYTPQVISIGPFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACS 120
           P   ++ +   Y PQ++SIGP+HHG   LM  E++K RA+   +AR    +E  +     
Sbjct: 115 PPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYIDAMKE 174

Query: 121 WEEKARSCYAHPIHMNKQDFAKMILLDGCFILE-FMNSIIIQRFCGEIDEDWIEYLLING 180
            EEKAR+CY  PI+MN+ +F +M++LDG FI+E F  +    +  G    D +    + G
Sbjct: 175 LEEKARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSEGFQEIGYAPNDPV--FGMRG 234

Query: 181 LMYDLKRDLTMLENQLPFFVLQRLFNLLPFPQNNDEDPNIYFMKCMEKLFIATTDQYEFP 240
           LM  ++RD+ MLENQLP+ VL+ L  L        + P++   K   +LF     Q   P
Sbjct: 235 LMQSIRRDMVMLENQLPWSVLKGLLQL--------QRPDV-LDKVNVQLF-QPFFQPLLP 294

Query: 241 SNLISSEDTVKHLVDLLRLFYLPSPET-EEYKKFIGTQGMTFIHPPTITELCDAGVKVQK 300
           +  + +E+   H +D+LR   L S  T +E    +  Q    IH   +TEL +AGV+  +
Sbjct: 295 TREVLTEEGGLHCLDVLRRGLLQSSGTSDEDMSMVNKQPQQLIH--CVTELRNAGVEFMR 354

Query: 301 AEKAKSFMDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFHYIGLLDG 360
            E    F D  FKNG LKIP  +IH   +    NLI  E  +        +  YI  +D 
Sbjct: 355 KETG-HFWDIEFKNGYLKIPKLLIHDGTKSLFLNLIAFE--QCHIKSSKKITSYIIFMDN 414

Query: 361 LINTEKDVSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSRDLNDYCN 420
           LIN+ +DVS L    II N + GSD +VS+L N L       P + Y + ++ ++N Y  
Sbjct: 415 LINSSEDVSYLHHYGIIENWL-GSDSEVSDLFNGLGKEVIFDPNDGYLSALTGEVNIYYR 474

Query: 421 NWWHRSIALLRRDYFNTPWASISFFAATFLLILTLLQTLFS 456
             W+   A LR  YFN PWA  SF AA  LLI T  Q+ F+
Sbjct: 475 RKWNYLKATLRHKYFNNPWAYFSFIAAVTLLIFTFCQSFFA 492

BLAST of Lsi02G021300 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 193.4 bits (490), Expect = 4.2e-49
Identity = 145/468 (30.98%), Postives = 234/468 (50.00%), Query Frame = 0

Query: 13  MMIMENNNKKKKKEEEEMGEVVISI----EEMLMELPAIN-QKRFMYKVPKLHRKINETV 72
           +M+ +   K+ +K EE   E VISI    E+ L E    +  K  +Y+VP+  ++ N+  
Sbjct: 96  LMMRQKLQKQNQKPEETREEWVISIRDKMEQALREDATTSWDKLCIYRVPQYLQENNKKS 155

Query: 73  YTPQVISIGPFHHGQPELMAAEQYKLRALKTYLARINMRVEEAMIMACSWEEKARSCYAH 132
           Y PQ +S+GPFHHG   L+  +++K RA+   +AR    +E  +      E++AR+CY  
Sbjct: 156 YFPQTVSLGPFHHGNKHLLPMDRHKWRAVNMVMARTKHDIEMYIDAMKELEDRARACYEG 215

Query: 133 PIHMNKQDFAKMILLDGCFILEFMNSIIIQRFCGEIDEDWIE-YLLINGLMYDLKRDLTM 192
           PI ++   F++M++LDGCF+LE            E+  D  +    + G M+ ++RD+ M
Sbjct: 216 PIDLSSNKFSEMLVLDGCFVLELFRG--ADEGFSELGYDRNDPVFAMRGSMHSIQRDMVM 275

Query: 193 LENQLPFFVLQRLFNLLPFPQNNDEDPNIYFMKCMEKLF-----IATTD----QYEFPSN 252
           LENQLP FVL RL  +    ++     +   ++  + L      +  TD    Q +F + 
Sbjct: 276 LENQLPLFVLNRLLEIQLGKRHQTGLVSRLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNP 335

Query: 253 LISSEDTVKHLVDLLRLFYL-----PSPETEEYK-----KFIGTQGMTFIHPPTITELCD 312
           +   +    H +D+ R   L     P P     +     +    +    IH   +TEL +
Sbjct: 336 IADKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRTRVADKRQQQLIH--CVTELRE 395

Query: 313 AGVKVQKAEKAKSFMDCSFKNGVLKIPPFIIHSNFEIHMRNLILSEIFRPGTTDHMYVFH 372
           AG+K  +  K   F D  FKNG L+IP  +IH   +    NLI  E     +++   +  
Sbjct: 396 AGIKF-RTRKTDRFWDIRFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSND--ITS 455

Query: 373 YIGLLDGLINTEKDVSILVKENIITNDIGGSDQQVSELINNLRLNAPTFPPNFYFNNMSR 432
           YI  +D LI++ +DV  L    II + + G+D +V++L N L       P N Y + +S 
Sbjct: 456 YIIFMDNLIDSSEDVRYLHYCGIIEHWL-GNDYEVADLFNRLCQEVAFDPQNSYLSQLSN 515

Query: 433 DLNDYCNNWWHRSIALLRRDYFNTPWASISFFAATFLLILTLLQTLFS 456
            ++   +  W+   A+L+  YFN PWA  SFFAA  LL+LTL Q+ F+
Sbjct: 516 KVDRNYSRKWNVLKAILKHKYFNNPWAYFSFFAALVLLVLTLFQSFFT 555

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD531.1e-3026.17UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8978.8e-1224.69Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A0A0K6U91.8e-14558.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G363030 PE=4 SV=1[more]
A0A6J1BVD41.1e-10048.98UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE... [more]
A0A6J1BQT61.9e-8944.22UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111004764 PE... [more]
A0A1S3BBL98.1e-8543.92UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488293 PE=4 SV=1[more]
A0A6J1DXD67.6e-8342.44UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_038899472.18.7e-16666.95UPF0481 protein At3g47200-like [Benincasa hispida][more]
XP_004149334.23.8e-14558.91UPF0481 protein At3g47200 [Cucumis sativus] >KGN44649.1 hypothetical protein Csa... [more]
XP_038896405.13.3e-14160.54UPF0481 protein At3g47200-like [Benincasa hispida][more]
XP_022132118.12.2e-10048.98UPF0481 protein At3g47200-like [Momordica charantia][more]
XP_022131634.13.9e-8944.22UPF0481 protein At3g47200-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G31980.16.8e-6034.56unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50170.13.9e-5534.57Plant protein of unknown function (DUF247) [more]
AT3G50150.12.4e-5234.80Plant protein of unknown function (DUF247) [more]
AT3G50160.11.3e-5034.06Plant protein of unknown function (DUF247) [more]
AT3G50130.14.2e-4930.98Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 54..447
e-value: 1.6E-99
score: 333.9
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 29..456
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 29..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G021300.1Lsi02G021300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane