Lsi02G020350 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G020350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWRKY protein
Locationchr02: 26389987 .. 26392231 (+)
RNA-Seq ExpressionLsi02G020350
SyntenyLsi02G020350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCACTGTACTGAATTTCCATTTCCTTGTTCACCAATCTTTTCCTTGTCTTTTCCTCTCAATTTCTTCAATCTCAATCATGGAGTTTCACTTTCTCTCTCCTCTTTCCTTTCTCTTCCTTTATAATCCCTTCCTTCTTCTCTTCTCTCAATTTCACTCTCTTCCTTCTTCCCCCCATTTCCATGCTCATTTCACACCTTCTCACCTTCCATCATGGAGTTTCTCACTTTAATTCAAGAGCTTAACCAAGGCAAAGAACTCGCTAACCAACTCCGCAACCATCTCCATCCTTCTTCTTCTTCTTCTTCCTCCGATGGCATTCTCTTGATCGATAAGATTCTTCGTTCTTACGAGAACGCGCTTTTGGCTCTCGCCGATGGTGGCGTCTCCGCGAAATCCGCCCTTGCTCCTGTGAATGCGGCTGTGAAAGACAGAGTTGTGTCCAAGAAGAGGTAATGATTTGTTTTTTAATGACTGGTCATTGCTAGGTCTTGTGGGTTTTGTTGATTTGGAAGAAATTCTTATTGGGTTTTGGGGGGTTTTGTTTTTTGCAGAAAGGTAATGGCGAAATGGAGTGAGCAGGTTAAGGTTTCTTCTGACTCTGCTAATGAAGGTCCTCGTTGTGATGGCTTCAGCTGGAGAAAGTATGGCCAGAAGGATATTCTTGGATCTAACTTCCCCAGGTCCTTGCCTCTTTAACTTATAATCTTCTTCTCTGCTTCTAATTCTGTTCATAATTTTGTTCCATTATGCATTATAAATGTATGAGTAAGAATGATCACTCATTTCTGAACCGTGTTCATGGTTCGTCTGTGCTTGGATTAGGGGCTTTTGTATGAAAAAAAAACTGTTGTTAAGCTCCGTTTCATGATCATTTAGTCCCCTTCTATTTGGTTCTTAGTTTTTTACTTTTGAAAATTAAGCTTAGTTTCTCTCAATTTCTTACAATGGTTTGCATCTAAAGAGTTGAATTTTTAGCCAAAATTCAAAAACAAAAATAAGTTTTCCAAGAGTTAGCGTGATTTTTGAAAACATTGATAAAAAGTAGATAACAAAGCAAGAAATTTAGAGGTAGAATGAATGTTTATGGGCTTAATTTTCAAAATGAAATGGTTACCGAATAGGGTCTGTTGTGCTGGTTTCTCTAAAAAATTGAACAATTTTGGATTTTATGGATCTGTATCTCTATTTCTTTGAATCAGAACATAGATTATTCCAGAGTTGATTGGAAAGGAATAAAATTGTGTTTGTATTTTTATGTATGTATGTATATCTAGGCTTTAGTTACTAATTTAGAACATTTTGTTATGTTTGGGTAATGCAGAGGCTATTTTAGATGCTCACATCGCTTTACACAAGGATGTTTAGCAACAAAACAAGTTCAAAAATCAGACAATGATCCAACAGTTTATGATATAACCTACAAAGGAAGACACACTTGCAACAGAGCCCATAATTCAAATAAAACACCACAAAAAGACCAAAACTCATTACTTCAACACCCAATGCCACCAAAACAAGAACAAAAGCCATTGCAGCAGCTACATGATCCTTTATGCTTTACGTTCAGCTCCGACGCCATTCGGGTAAAATCTGAAAACTTAGATGATGTCGATGGTGGCCTCTTTCGACCATTTTGTTCCCCGTCCCCAATGTTTGGATCTGAAGTTCAAGATGATCACAGTCCTTTTAGGGAAAGTGAGTTGTCTCCGACGTTTGAATCAAATGATATATTTGGATTCTGTTGTGATTTCGAAACCGGGTTCGTCTCTGTTCCTAATTCAATGACTAATATTTCTATTGGAGACTTGGAGGAGTATTGTAGTTTTGATAACTTGGAGTTGTTCTGTTGAAATTGTTTCCTCACCTTCCAGACAAAACAAGAGGGAAAGAAAAGAAAAGATTGTGAATTAGTATAAGTCAGCATTGAAAGAGAGATGATGGATAATATCAAAGGCCTCCTGCATTGCTGGTTAGAGATTCTTTTTTTTTTTTTTTTCCCTTTTTTTTCCTCTCTATCTCTCTGTTCTTTTTTAATTTTCTTTTTATTCTTCTGTGGATCCTTTGGGAGGCCAAAATTTCAGTGTAATTATTGATCTTAGTGTAGGAGAAAATAGGATTCTGGCAAGTTTCTTAGCAGTTGTGTATTTGAATTAGTGAAGAATAAGATTGAATTTAAATACTAAATATTAATGTCACAGTATTCGTTATTTTCAAATATAGAAAAATGAGTCAAAATATTTAT

mRNA sequence

CCCCACTGTACTGAATTTCCATTTCCTTGTTCACCAATCTTTTCCTTGTCTTTTCCTCTCAATTTCTTCAATCTCAATCATGGAGTTTCACTTTCTCTCTCCTCTTTCCTTTCTCTTCCTTTATAATCCCTTCCTTCTTCTCTTCTCTCAATTTCACTCTCTTCCTTCTTCCCCCCATTTCCATGCTCATTTCACACCTTCTCACCTTCCATCATGGAGTTTCTCACTTTAATTCAAGAGCTTAACCAAGGCAAAGAACTCGCTAACCAACTCCGCAACCATCTCCATCCTTCTTCTTCTTCTTCTTCCTCCGATGGCATTCTCTTGATCGATAAGATTCTTCGTTCTTACGAGAACGCGCTTTTGGCTCTCGCCGATGGTGGCGTCTCCGCGAAATCCGCCCTTGCTCCTGTGAATGCGGCTGTGAAAGACAGAGTTGTGTCCAAGAAGAGAAAGGTAATGGCGAAATGGAGTGAGCAGGTTAAGGTTTCTTCTGACTCTGCTAATGAAGGTCCTCGTTGTGATGGCTTCAGCTGGAGAAAGTATGGCCAGAAGGATATTCTTGGATCTAACTTCCCCAGAGGCTATTTTAGATGCTCACATCGCTTTACACAAGGATGTTTAGCAACAAAACAAGTTCAAAAATCAGACAATGATCCAACAGTTTATGATATAACCTACAAAGGAAGACACACTTGCAACAGAGCCCATAATTCAAATAAAACACCACAAAAAGACCAAAACTCATTACTTCAACACCCAATGCCACCAAAACAAGAACAAAAGCCATTGCAGCAGCTACATGATCCTTTATGCTTTACGTTCAGCTCCGACGCCATTCGGGTAAAATCTGAAAACTTAGATGATGTCGATGGTGGCCTCTTTCGACCATTTTGTTCCCCGTCCCCAATGTTTGGATCTGAAGTTCAAGATGATCACAGTCCTTTTAGGGAAAGTGAGTTGTCTCCGACGTTTGAATCAAATGATATATTTGGATTCTGTTGTGATTTCGAAACCGGGTTCGTCTCTGTTCCTAATTCAATGACTAATATTTCTATTGGAGACTTGGAGGAGTATTGTAGTTTTGATAACTTGGAGTTGTTCTGTTGAAATTGTTTCCTCACCTTCCAGACAAAACAAGAGGGAAAGAAAAGAAAAGATTGTGAATTAGTATAAGTCAGCATTGAAAGAGAGATGATGGATAATATCAAAGGCCTCCTGCATTGCTGGTTAGAGATTCTTTTTTTTTTTTTTTTCCCTTTTTTTTCCTCTCTATCTCTCTGTTCTTTTTTAATTTTCTTTTTATTCTTCTGTGGATCCTTTGGGAGGCCAAAATTTCAGTGTAATTATTGATCTTAGTGTAGGAGAAAATAGGATTCTGGCAAGTTTCTTAGCAGTTGTGTATTTGAATTAGTGAAGAATAAGATTGAATTTAAATACTAAATATTAATGTCACAGTATTCGTTATTTTCAAATATAGAAAAATGAGTCAAAATATTTAT

Coding sequence (CDS)

ATGGAGTTTCTCACTTTAATTCAAGAGCTTAACCAAGGCAAAGAACTCGCTAACCAACTCCGCAACCATCTCCATCCTTCTTCTTCTTCTTCTTCCTCCGATGGCATTCTCTTGATCGATAAGATTCTTCGTTCTTACGAGAACGCGCTTTTGGCTCTCGCCGATGGTGGCGTCTCCGCGAAATCCGCCCTTGCTCCTGTGAATGCGGCTGTGAAAGACAGAGTTGTGTCCAAGAAGAGAAAGGTAATGGCGAAATGGAGTGAGCAGGTTAAGGTTTCTTCTGACTCTGCTAATGAAGGTCCTCGTTGTGATGGCTTCAGCTGGAGAAAGTATGGCCAGAAGGATATTCTTGGATCTAACTTCCCCAGAGGCTATTTTAGATGCTCACATCGCTTTACACAAGGATGTTTAGCAACAAAACAAGTTCAAAAATCAGACAATGATCCAACAGTTTATGATATAACCTACAAAGGAAGACACACTTGCAACAGAGCCCATAATTCAAATAAAACACCACAAAAAGACCAAAACTCATTACTTCAACACCCAATGCCACCAAAACAAGAACAAAAGCCATTGCAGCAGCTACATGATCCTTTATGCTTTACGTTCAGCTCCGACGCCATTCGGGTAAAATCTGAAAACTTAGATGATGTCGATGGTGGCCTCTTTCGACCATTTTGTTCCCCGTCCCCAATGTTTGGATCTGAAGTTCAAGATGATCACAGTCCTTTTAGGGAAAGTGAGTTGTCTCCGACGTTTGAATCAAATGATATATTTGGATTCTGTTGTGATTTCGAAACCGGGTTCGTCTCTGTTCCTAATTCAATGACTAATATTTCTATTGGAGACTTGGAGGAGTATTGTAGTTTTGATAACTTGGAGTTGTTCTGTTGA

Protein sequence

MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALADGGVSAKSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGSNFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSLLQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQDDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLELFC
Homology
BLAST of Lsi02G020350 vs. ExPASy Swiss-Prot
Match: Q9SUP6 (Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.8e-32
Identity = 111/322 (34.47%), Postives = 156/322 (48.45%), Query Frame = 0

Query: 5   TLIQELNQG----KELANQLRNHLHPSSSSSS------SDGILLIDKILRSYENALLAL- 64
           TL+ EL  G    K+L  +LR    PSSS SS          +L+ +I+ SYE +LL L 
Sbjct: 13  TLLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSLLLLN 72

Query: 65  -----------------------------------------ADGGVSAKSALAPVNAAVK 124
                                                    ADGG S++S         +
Sbjct: 73  WSSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHR------Q 132

Query: 125 DRVV-SKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGSNFPRGYFRCSHR 184
           D +  SKKRK++ KWSE+V++S +   EGP+ D FSWRKYGQKDILG+ FPR Y+RC+HR
Sbjct: 133 DYIFNSKKRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFPRSYYRCTHR 192

Query: 185 FTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSN---KTPQKDQNSLLQHPMPPKQ 244
            TQ C ATKQVQ+SD D TV+++TY+G HTC++A        +P+K Q++ ++    P  
Sbjct: 193 STQNCWATKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPLASPEKRQDTRVK----PAI 252

Query: 245 EQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQDDHSPFRES 271
            QKP   L            + V+++ LDD       P   P   +G+     +  F   
Sbjct: 253 TQKPKDILES------LKSNLTVRTDGLDDGKDVFSFPDTPPFYNYGT----INGEFGHV 312

BLAST of Lsi02G020350 vs. ExPASy Swiss-Prot
Match: Q9SKD9 (Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.0e-29
Identity = 84/176 (47.73%), Postives = 108/176 (61.36%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALA---DGG 60
           ME   +I EL  GKELAN+L N+L  +SS  S+    LI  ILR Y+NA+  L+   D  
Sbjct: 3   MEEKLVINELELGKELANRLMNNLKHTSSVDSNK--TLISDILRIYQNAIFMLSFNQDKN 62

Query: 61  VSAKSALAPVNAAVKD-RVVSKKRKVMAKWSEQVKVSSDSANE-GPRCDGFSWRKYGQKD 120
           +  +S    +    KD + V KKRKV  K +E+VKV   +  E G   DG  WRKYGQK+
Sbjct: 63  ILKRS----LEIDGKDSKNVFKKRKVSEKNTEKVKVFVATEQENGSIDDGHCWRKYGQKE 122

Query: 121 ILGSNFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKT 172
           I GS  PR Y+RC+HRFTQ CLA KQVQKSD DP+++++ Y G HTCN   +   T
Sbjct: 123 IHGSKNPRAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHTCNNITSPKTT 172

BLAST of Lsi02G020350 vs. ExPASy Swiss-Prot
Match: Q8H0Y8 (Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 PE=1 SV=2)

HSP 1 Score: 131.0 bits (328), Expect = 2.3e-29
Identity = 86/222 (38.74%), Postives = 120/222 (54.05%), Query Frame = 0

Query: 5   TLIQELNQGKELANQLRNHLHPSSSSSSSDGIL-----LIDKILRSYENALLALADGGVS 64
           +L+ EL  G + A QL+    PS S+SSS         L+  I+ S++ A+L L +G  +
Sbjct: 10  SLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILML-NGSTT 69

Query: 65  AKS---ALAP-------------------------VNAAVKD-RVVSKKRKVMAKWSEQV 124
             +    LAP                          N   K+  + SKKRK++ KW+EQV
Sbjct: 70  QHNPTIELAPDPLAHPGKVPGSPASITGNPRSEEFFNVRSKEFNLSSKKRKMLPKWTEQV 129

Query: 125 KVSSDSANEGPRCDGFSWRKYGQKDILGSNFPRGYFRCSHRFTQGCLATKQVQKSDNDPT 184
           ++S +   EGP  D FSWRKYGQKDILG+ FPR Y+RC+ R TQ C ATKQVQ+SD DPT
Sbjct: 130 RISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQRSDGDPT 189

Query: 185 VYDITYKGRHTCNRAHNSNKTPQKDQNSLLQHPMPPKQEQKP 193
           ++++TY+G HTC++                  P+P K+E KP
Sbjct: 190 IFEVTYRGTHTCSQG----------------IPLPEKRETKP 214

BLAST of Lsi02G020350 vs. ExPASy Swiss-Prot
Match: Q9FL62 (Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.0e-25
Identity = 68/171 (39.77%), Postives = 104/171 (60.82%), Query Frame = 0

Query: 11  NQGKELANQLRNHLHPSSSSSSSD-GILLIDKILRSYENALLAL---------ADGGVSA 70
           N+  EL  + R++ H   S+SS +    L  KIL+SY  +L  +         + GG S 
Sbjct: 15  NEINELMIEGRDYAHQFGSASSQETREHLAKKILQSYHKSLTIMNYSGELDQVSQGGGSP 74

Query: 71  KSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGSN 130
           KS     ++  +  V+   +K M +WS +V+++  +  +    DGFSWRKYGQKDILG+ 
Sbjct: 75  KSD----DSDQEPLVIKSSKKSMPRWSSKVRIAPGAGVDRTLDDGFSWRKYGQKDILGAK 134

Query: 131 FPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKT 172
           FPRGY+RC++R +QGC ATKQVQ+SD +  + +I+Y+G H+C++A N   T
Sbjct: 135 FPRGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYRGIHSCSQAANVGTT 181

BLAST of Lsi02G020350 vs. ExPASy Swiss-Prot
Match: Q0DFT7 (Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.1e-22
Identity = 94/274 (34.31%), Postives = 126/274 (45.99%), Query Frame = 0

Query: 4   LTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALADGGVSAKSA 63
           + L  EL Q + +A +L   + P   ++       + + L S  +  + LA         
Sbjct: 13  IMLATELAQLRAMARELEAKMDPDRVAARE-----LCRALASSVDRSIRLAASCFPPPEH 72

Query: 64  LAPVNAAVKDRVVSKKRKVMAKWSEQVKVSS--DSANEGPRCDGFSWRKYGQKDILGSNF 123
             P           KKRK MAK   QV+V+S  D+A+     DG SWRKYGQKDILG+ +
Sbjct: 73  PPPAAGNAGRDAAFKKRKGMAKVRRQVRVTSVQDTASLD---DGLSWRKYGQKDILGAKY 132

Query: 124 PRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNR---AHNSNKTP------ 183
           PR YFRC+HR TQGC ATKQVQ++D DP ++D+ Y G HTC +   A  +   P      
Sbjct: 133 PRAYFRCTHRHTQGCNATKQVQRADGDPLLFDVVYLGDHTCGQAAVAAAAQSAPPEHAGQ 192

Query: 184 -QKDQNSLLQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPS 243
            Q+ Q+SLL        +Q   + +  P  FT         S     VD G F  F SP+
Sbjct: 193 EQQRQSSLLAAGTEGIHQQVVAEPMAAPFLFT---------STAAGGVDDGYF-SFISPA 252

Query: 244 P---MFGSE-------VQDDHSPFRESELSPTFE 256
                F S+       V  DH    E   S T E
Sbjct: 253 NSDCQFSSDFSAGSVGVDMDHEARFEDLFSSTLE 268

BLAST of Lsi02G020350 vs. ExPASy TrEMBL
Match: A0A5D3C947 (Putative WRKY transcription factor 53 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1737G00520 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 6.7e-141
Identity = 251/299 (83.95%), Postives = 270/299 (90.30%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLAL-ADGGVS 60
           MEFL+LIQELNQGK+LANQLRNHLHP   SSSS GILL+DKILRSYENALLAL A GG S
Sbjct: 1   MEFLSLIQELNQGKQLANQLRNHLHP---SSSSHGILLVDKILRSYENALLALSAGGGAS 60

Query: 61  AKSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGS 120
             SA+APVNAAVKD  VSKKRK+MAKWSEQVKVSS SA EGP CDGFSWRKYGQKDILGS
Sbjct: 61  VNSAIAPVNAAVKDGDVSKKRKLMAKWSEQVKVSSGSAVEGPGCDGFSWRKYGQKDILGS 120

Query: 121 NFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSL 180
            FPRGYFRCSHRFTQGCLATKQVQKSDNDPT+YD+TY+GRHTCN+A +S  TPQ+ QNSL
Sbjct: 121 QFPRGYFRCSHRFTQGCLATKQVQKSDNDPTIYDVTYRGRHTCNKALHSTNTPQEHQNSL 180

Query: 181 LQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQ 240
           LQHP+PPKQE+KPLQQLHDPLCF FSSD IRVKSENLD+ +GGL +PFC PSPMFGSEVQ
Sbjct: 181 LQHPIPPKQEEKPLQQLHDPLCFMFSSDPIRVKSENLDNANGGLLQPFCPPSPMFGSEVQ 240

Query: 241 DDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLELFC 299
           DD SPFRESE S TFESND FG CCDFETGFVS+P+SMTNISIGDLEEYCSFDNLE+FC
Sbjct: 241 DDQSPFRESECSLTFESNDTFGLCCDFETGFVSIPSSMTNISIGDLEEYCSFDNLEMFC 296

BLAST of Lsi02G020350 vs. ExPASy TrEMBL
Match: A0A1S3CGE2 (probable WRKY transcription factor 53 OS=Cucumis melo OX=3656 GN=LOC103500461 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 8.8e-141
Identity = 251/299 (83.95%), Postives = 270/299 (90.30%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLAL-ADGGVS 60
           MEFL+LIQELNQGK+LANQLRNHLHP   SSSS GILL+DKILRSYENALLAL A GG S
Sbjct: 1   MEFLSLIQELNQGKQLANQLRNHLHP---SSSSHGILLVDKILRSYENALLALSAGGGAS 60

Query: 61  AKSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGS 120
             SA+APVNAAVKD  VSKKRK+MAKWSEQVKVSS SA EGP CDGFSWRKYGQKDILGS
Sbjct: 61  VNSAIAPVNAAVKDGDVSKKRKLMAKWSEQVKVSSGSAVEGPCCDGFSWRKYGQKDILGS 120

Query: 121 NFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSL 180
            FPRGYFRCSHRFTQGCLATKQVQKSDNDPT+YD+TY+GRHTCN+A +S  TPQ+ QNSL
Sbjct: 121 QFPRGYFRCSHRFTQGCLATKQVQKSDNDPTIYDVTYRGRHTCNKALHSTNTPQEHQNSL 180

Query: 181 LQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQ 240
           LQHP+PPKQE+KPLQQLHDPLCF FSSD IRVKSENLD+ +GGL +PFC PSPMFGSEVQ
Sbjct: 181 LQHPIPPKQEEKPLQQLHDPLCFMFSSDPIRVKSENLDNANGGLLQPFCPPSPMFGSEVQ 240

Query: 241 DDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLELFC 299
           DD SPFRESE S TFESND FG CCDFETGFVS+P+SMTNISIGDLEEYCSFDNLE+FC
Sbjct: 241 DDQSPFRESECSLTFESNDTFGLCCDFETGFVSIPSSMTNISIGDLEEYCSFDNLEMFC 296

BLAST of Lsi02G020350 vs. ExPASy TrEMBL
Match: E7CEY4 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY50 PE=2 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.8e-131
Identity = 240/299 (80.27%), Postives = 262/299 (87.63%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALADG-GVS 60
           MEFL+LIQELNQGK+LANQLR HLHP   SSSS GILLIDKILRSYENALLAL+ G   S
Sbjct: 1   MEFLSLIQELNQGKQLANQLRTHLHP---SSSSHGILLIDKILRSYENALLALSGGAAAS 60

Query: 61  AKSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGS 120
             SA+APVNAAV+D  VSKKRKVMAKWSEQVKVSS SA EGP CDGFSWRKYGQKDILGS
Sbjct: 61  VNSAIAPVNAAVQDGDVSKKRKVMAKWSEQVKVSSASAVEGPGCDGFSWRKYGQKDILGS 120

Query: 121 NFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSL 180
            FPR YFRCSHRFTQGCLATKQVQKSDNDPT+Y++TYKGRHTCN+A +S  TPQ+ QN+ 
Sbjct: 121 KFPRSYFRCSHRFTQGCLATKQVQKSDNDPTIYEVTYKGRHTCNKALHSTNTPQEHQNTF 180

Query: 181 LQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQ 240
           LQHP+PPKQE KPLQQLHDPLCF FSSD IRVKSE+L+  +GGLF+P  +PSPMFGSEVQ
Sbjct: 181 LQHPIPPKQEDKPLQQLHDPLCFMFSSDPIRVKSEDLEYANGGLFQPLRTPSPMFGSEVQ 240

Query: 241 DDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLELFC 299
           DD SPFRESE SPTFESND+FG  CDFET FVS+P+SMTNISIGDLEE  SFDNLE+FC
Sbjct: 241 DDLSPFRESECSPTFESNDMFGLWCDFETEFVSIPSSMTNISIGDLEECFSFDNLEMFC 296

BLAST of Lsi02G020350 vs. ExPASy TrEMBL
Match: A0A6J1DUY2 (probable WRKY transcription factor 46 OS=Momordica charantia OX=3673 GN=LOC111024269 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.4e-101
Identity = 214/311 (68.81%), Postives = 231/311 (74.28%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALADGGVSA 60
           ME  TLIQELNQGKELA QLRNHL P SSS+   GILLIDKIL SYE AL AL  G V+A
Sbjct: 13  MEQRTLIQELNQGKELAEQLRNHLRPPSSSA---GILLIDKILSSYEKALSALG-GEVTA 72

Query: 61  KSALAPVNAAVKDRVVSKKRKVM--AKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILG 120
                   AA K  VVSKKRKVM  +KWSEQVKV S +  E P  DGFSWRKYGQKDILG
Sbjct: 73  --------AAPKSAVVSKKRKVMGKSKWSEQVKVPSGAGLEAPLGDGFSWRKYGQKDILG 132

Query: 121 SNFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNS 180
           S FPRGYFRCSHR+ QGCLATKQVQKSD DPT+YDITYKG HTCNR  +S  T  ++Q  
Sbjct: 133 SKFPRGYFRCSHRYAQGCLATKQVQKSDKDPTIYDITYKGMHTCNRVLDSVTTKPQEQKP 192

Query: 181 LLQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEV 240
           L+QHPMPPKQEQ PLQ  HDP C  F  DA+RVKSEN DDVDGG+FR F  PSP FGSEV
Sbjct: 193 LIQHPMPPKQEQMPLQ--HDPSCSGFGVDAVRVKSENPDDVDGGVFR-FRVPSPWFGSEV 252

Query: 241 QDDHSPFRESE----LSP-TFESNDIFGFC------CDFETGFVSVPNSMTNISIGDLEE 299
           + DHSPF ESE    LSP TFES D FG C       D E GFVSVP+S +N+ IGD+EE
Sbjct: 253 RGDHSPFEESELGGVLSPTTFESRDAFGLCYIGDPAWDSEFGFVSVPHSASNVPIGDMEE 308

BLAST of Lsi02G020350 vs. ExPASy TrEMBL
Match: A0A6J1JVH2 (probable WRKY transcription factor 46 OS=Cucurbita maxima OX=3661 GN=LOC111488691 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.4e-98
Identity = 203/303 (67.00%), Postives = 227/303 (74.92%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALADGGVSA 60
           ME + LIQEL+QGK LANQLR HLHPSSSS +   ILLID+ILRSY+NAL +LA      
Sbjct: 1   MELMALIQELSQGKHLANQLRTHLHPSSSSHA---ILLIDQILRSYDNALFSLA------ 60

Query: 61  KSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGSN 120
                P NA VKDR +++KRKVM KWSEQVKV+S SA EGP  DGFSWRKYGQKDILGS 
Sbjct: 61  --LPVPTNAPVKDRHIARKRKVMPKWSEQVKVASASAVEGPLHDGFSWRKYGQKDILGSK 120

Query: 121 FPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSLL 180
           FPRGYFRCSHRFT GC ATKQVQKSDNDPT+Y++TYKG HTCNR  +SN T Q+ Q   L
Sbjct: 121 FPRGYFRCSHRFTLGCKATKQVQKSDNDPTIYEVTYKGTHTCNRPLHSN-TQQQGQKPSL 180

Query: 181 QHPMPPKQEQKPLQQLHDPLCFTFSSD--AIRVKSENLD--DVDGGLFRPFCSPSPMFGS 240
           QHPMPPKQEQ+P     +P C +FS +  AI VKSE  D  D+D GLFRP C PS +F S
Sbjct: 181 QHPMPPKQEQRPDDD--EPSCQSFSPEVAAIHVKSETFDDIDIDNGLFRPLCPPSSLFES 240

Query: 241 EVQ-DDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLE 299
           EVQ DD SPFRESEL         FG CCDFETG VS+PNS+TNI I +LEEYCSFDNLE
Sbjct: 241 EVQDDDQSPFRESEL---------FGLCCDFETGVVSIPNSVTNICIEELEEYCSFDNLE 279

BLAST of Lsi02G020350 vs. NCBI nr
Match: XP_038899647.1 (probable WRKY transcription factor 53 [Benincasa hispida])

HSP 1 Score: 515.8 bits (1327), Expect = 2.5e-142
Identity = 260/303 (85.81%), Postives = 277/303 (91.42%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHP--SSSSSSSDGILLIDKILRSYENALLALADGGV 60
           MEFLTLIQELNQGK+LANQLRNHLHP  SSSSSSSDGILLIDKIL SYENALLALA GG 
Sbjct: 1   MEFLTLIQELNQGKQLANQLRNHLHPSSSSSSSSSDGILLIDKILHSYENALLALAGGGA 60

Query: 61  SA---KSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKD 120
           +A   KSALAP+N    DR VSKKRKVM KWSEQVKVSSDSA +GPRCDGFSWRKYGQKD
Sbjct: 61  AAASVKSALAPLN----DRGVSKKRKVMVKWSEQVKVSSDSAIDGPRCDGFSWRKYGQKD 120

Query: 121 ILGSNFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKD 180
           ILGS FPRGYFRCSHRFTQGCLATKQVQKSD+DPT+YD+TYKGRHTCN+A +SN TPQ+D
Sbjct: 121 ILGSKFPRGYFRCSHRFTQGCLATKQVQKSDDDPTIYDVTYKGRHTCNKALHSN-TPQED 180

Query: 181 QNSLLQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFG 240
            NSLLQHPMPPKQEQKPLQQLHDP  FTFSSDAIRVKSENLDDVDGGLF+ FC  SPMFG
Sbjct: 181 PNSLLQHPMPPKQEQKPLQQLHDPSSFTFSSDAIRVKSENLDDVDGGLFQQFCDLSPMFG 240

Query: 241 SEVQDDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLE 299
           SEVQDD SPFRESELSPTFESND+FGFCCDFETGF+S+PNS+TNISIGDLEEY SFDNL+
Sbjct: 241 SEVQDDQSPFRESELSPTFESNDMFGFCCDFETGFISIPNSVTNISIGDLEEYFSFDNLD 298

BLAST of Lsi02G020350 vs. NCBI nr
Match: KAA0050658.1 (putative WRKY transcription factor 53 [Cucumis melo var. makuwa] >TYK07774.1 putative WRKY transcription factor 53 [Cucumis melo var. makuwa])

HSP 1 Score: 510.0 bits (1312), Expect = 1.4e-140
Identity = 251/299 (83.95%), Postives = 270/299 (90.30%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLAL-ADGGVS 60
           MEFL+LIQELNQGK+LANQLRNHLHP   SSSS GILL+DKILRSYENALLAL A GG S
Sbjct: 1   MEFLSLIQELNQGKQLANQLRNHLHP---SSSSHGILLVDKILRSYENALLALSAGGGAS 60

Query: 61  AKSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGS 120
             SA+APVNAAVKD  VSKKRK+MAKWSEQVKVSS SA EGP CDGFSWRKYGQKDILGS
Sbjct: 61  VNSAIAPVNAAVKDGDVSKKRKLMAKWSEQVKVSSGSAVEGPGCDGFSWRKYGQKDILGS 120

Query: 121 NFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSL 180
            FPRGYFRCSHRFTQGCLATKQVQKSDNDPT+YD+TY+GRHTCN+A +S  TPQ+ QNSL
Sbjct: 121 QFPRGYFRCSHRFTQGCLATKQVQKSDNDPTIYDVTYRGRHTCNKALHSTNTPQEHQNSL 180

Query: 181 LQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQ 240
           LQHP+PPKQE+KPLQQLHDPLCF FSSD IRVKSENLD+ +GGL +PFC PSPMFGSEVQ
Sbjct: 181 LQHPIPPKQEEKPLQQLHDPLCFMFSSDPIRVKSENLDNANGGLLQPFCPPSPMFGSEVQ 240

Query: 241 DDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLELFC 299
           DD SPFRESE S TFESND FG CCDFETGFVS+P+SMTNISIGDLEEYCSFDNLE+FC
Sbjct: 241 DDQSPFRESECSLTFESNDTFGLCCDFETGFVSIPSSMTNISIGDLEEYCSFDNLEMFC 296

BLAST of Lsi02G020350 vs. NCBI nr
Match: XP_008461982.1 (PREDICTED: probable WRKY transcription factor 53 [Cucumis melo])

HSP 1 Score: 509.6 bits (1311), Expect = 1.8e-140
Identity = 251/299 (83.95%), Postives = 270/299 (90.30%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLAL-ADGGVS 60
           MEFL+LIQELNQGK+LANQLRNHLHP   SSSS GILL+DKILRSYENALLAL A GG S
Sbjct: 1   MEFLSLIQELNQGKQLANQLRNHLHP---SSSSHGILLVDKILRSYENALLALSAGGGAS 60

Query: 61  AKSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGS 120
             SA+APVNAAVKD  VSKKRK+MAKWSEQVKVSS SA EGP CDGFSWRKYGQKDILGS
Sbjct: 61  VNSAIAPVNAAVKDGDVSKKRKLMAKWSEQVKVSSGSAVEGPCCDGFSWRKYGQKDILGS 120

Query: 121 NFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSL 180
            FPRGYFRCSHRFTQGCLATKQVQKSDNDPT+YD+TY+GRHTCN+A +S  TPQ+ QNSL
Sbjct: 121 QFPRGYFRCSHRFTQGCLATKQVQKSDNDPTIYDVTYRGRHTCNKALHSTNTPQEHQNSL 180

Query: 181 LQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQ 240
           LQHP+PPKQE+KPLQQLHDPLCF FSSD IRVKSENLD+ +GGL +PFC PSPMFGSEVQ
Sbjct: 181 LQHPIPPKQEEKPLQQLHDPLCFMFSSDPIRVKSENLDNANGGLLQPFCPPSPMFGSEVQ 240

Query: 241 DDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLELFC 299
           DD SPFRESE S TFESND FG CCDFETGFVS+P+SMTNISIGDLEEYCSFDNLE+FC
Sbjct: 241 DDQSPFRESECSLTFESNDTFGLCCDFETGFVSIPSSMTNISIGDLEEYCSFDNLEMFC 296

BLAST of Lsi02G020350 vs. NCBI nr
Match: NP_001267693.1 (probable WRKY transcription factor 46-like [Cucumis sativus] >ADU52527.1 WRKY protein [Cucumis sativus] >KGN44546.1 hypothetical protein Csa_015970 [Cucumis sativus])

HSP 1 Score: 477.2 bits (1227), Expect = 1.0e-130
Identity = 240/299 (80.27%), Postives = 262/299 (87.63%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALADG-GVS 60
           MEFL+LIQELNQGK+LANQLR HLHP   SSSS GILLIDKILRSYENALLAL+ G   S
Sbjct: 1   MEFLSLIQELNQGKQLANQLRTHLHP---SSSSHGILLIDKILRSYENALLALSGGAAAS 60

Query: 61  AKSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGS 120
             SA+APVNAAV+D  VSKKRKVMAKWSEQVKVSS SA EGP CDGFSWRKYGQKDILGS
Sbjct: 61  VNSAIAPVNAAVQDGDVSKKRKVMAKWSEQVKVSSASAVEGPGCDGFSWRKYGQKDILGS 120

Query: 121 NFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSL 180
            FPR YFRCSHRFTQGCLATKQVQKSDNDPT+Y++TYKGRHTCN+A +S  TPQ+ QN+ 
Sbjct: 121 KFPRSYFRCSHRFTQGCLATKQVQKSDNDPTIYEVTYKGRHTCNKALHSTNTPQEHQNTF 180

Query: 181 LQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQ 240
           LQHP+PPKQE KPLQQLHDPLCF FSSD IRVKSE+L+  +GGLF+P  +PSPMFGSEVQ
Sbjct: 181 LQHPIPPKQEDKPLQQLHDPLCFMFSSDPIRVKSEDLEYANGGLFQPLRTPSPMFGSEVQ 240

Query: 241 DDHSPFRESELSPTFESNDIFGFCCDFETGFVSVPNSMTNISIGDLEEYCSFDNLELFC 299
           DD SPFRESE SPTFESND+FG  CDFET FVS+P+SMTNISIGDLEE  SFDNLE+FC
Sbjct: 241 DDLSPFRESECSPTFESNDMFGLWCDFETEFVSIPSSMTNISIGDLEECFSFDNLEMFC 296

BLAST of Lsi02G020350 vs. NCBI nr
Match: XP_022157612.1 (probable WRKY transcription factor 46 [Momordica charantia])

HSP 1 Score: 379.4 bits (973), Expect = 2.8e-101
Identity = 214/311 (68.81%), Postives = 231/311 (74.28%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALADGGVSA 60
           ME  TLIQELNQGKELA QLRNHL P SSS+   GILLIDKIL SYE AL AL  G V+A
Sbjct: 13  MEQRTLIQELNQGKELAEQLRNHLRPPSSSA---GILLIDKILSSYEKALSALG-GEVTA 72

Query: 61  KSALAPVNAAVKDRVVSKKRKVM--AKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILG 120
                   AA K  VVSKKRKVM  +KWSEQVKV S +  E P  DGFSWRKYGQKDILG
Sbjct: 73  --------AAPKSAVVSKKRKVMGKSKWSEQVKVPSGAGLEAPLGDGFSWRKYGQKDILG 132

Query: 121 SNFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNS 180
           S FPRGYFRCSHR+ QGCLATKQVQKSD DPT+YDITYKG HTCNR  +S  T  ++Q  
Sbjct: 133 SKFPRGYFRCSHRYAQGCLATKQVQKSDKDPTIYDITYKGMHTCNRVLDSVTTKPQEQKP 192

Query: 181 LLQHPMPPKQEQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEV 240
           L+QHPMPPKQEQ PLQ  HDP C  F  DA+RVKSEN DDVDGG+FR F  PSP FGSEV
Sbjct: 193 LIQHPMPPKQEQMPLQ--HDPSCSGFGVDAVRVKSENPDDVDGGVFR-FRVPSPWFGSEV 252

Query: 241 QDDHSPFRESE----LSP-TFESNDIFGFC------CDFETGFVSVPNSMTNISIGDLEE 299
           + DHSPF ESE    LSP TFES D FG C       D E GFVSVP+S +N+ IGD+EE
Sbjct: 253 RGDHSPFEESELGGVLSPTTFESRDAFGLCYIGDPAWDSEFGFVSVPHSASNVPIGDMEE 308

BLAST of Lsi02G020350 vs. TAIR 10
Match: AT4G23810.1 (WRKY family transcription factor )

HSP 1 Score: 140.2 bits (352), Expect = 2.7e-33
Identity = 111/322 (34.47%), Postives = 156/322 (48.45%), Query Frame = 0

Query: 5   TLIQELNQG----KELANQLRNHLHPSSSSSS------SDGILLIDKILRSYENALLAL- 64
           TL+ EL  G    K+L  +LR    PSSS SS          +L+ +I+ SYE +LL L 
Sbjct: 13  TLLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSLLLLN 72

Query: 65  -----------------------------------------ADGGVSAKSALAPVNAAVK 124
                                                    ADGG S++S         +
Sbjct: 73  WSSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHR------Q 132

Query: 125 DRVV-SKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGSNFPRGYFRCSHR 184
           D +  SKKRK++ KWSE+V++S +   EGP+ D FSWRKYGQKDILG+ FPR Y+RC+HR
Sbjct: 133 DYIFNSKKRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFPRSYYRCTHR 192

Query: 185 FTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSN---KTPQKDQNSLLQHPMPPKQ 244
            TQ C ATKQVQ+SD D TV+++TY+G HTC++A        +P+K Q++ ++    P  
Sbjct: 193 STQNCWATKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPLASPEKRQDTRVK----PAI 252

Query: 245 EQKPLQQLHDPLCFTFSSDAIRVKSENLDDVDGGLFRPFCSPSPMFGSEVQDDHSPFRES 271
            QKP   L            + V+++ LDD       P   P   +G+     +  F   
Sbjct: 253 TQKPKDILES------LKSNLTVRTDGLDDGKDVFSFPDTPPFYNYGT----INGEFGHV 312

BLAST of Lsi02G020350 vs. TAIR 10
Match: AT2G46400.1 (WRKY DNA-binding protein 46 )

HSP 1 Score: 132.1 bits (331), Expect = 7.3e-31
Identity = 84/176 (47.73%), Postives = 108/176 (61.36%), Query Frame = 0

Query: 1   MEFLTLIQELNQGKELANQLRNHLHPSSSSSSSDGILLIDKILRSYENALLALA---DGG 60
           ME   +I EL  GKELAN+L N+L  +SS  S+    LI  ILR Y+NA+  L+   D  
Sbjct: 3   MEEKLVINELELGKELANRLMNNLKHTSSVDSNK--TLISDILRIYQNAIFMLSFNQDKN 62

Query: 61  VSAKSALAPVNAAVKD-RVVSKKRKVMAKWSEQVKVSSDSANE-GPRCDGFSWRKYGQKD 120
           +  +S    +    KD + V KKRKV  K +E+VKV   +  E G   DG  WRKYGQK+
Sbjct: 63  ILKRS----LEIDGKDSKNVFKKRKVSEKNTEKVKVFVATEQENGSIDDGHCWRKYGQKE 122

Query: 121 ILGSNFPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKT 172
           I GS  PR Y+RC+HRFTQ CLA KQVQKSD DP+++++ Y G HTCN   +   T
Sbjct: 123 IHGSKNPRAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHTCNNITSPKTT 172

BLAST of Lsi02G020350 vs. TAIR 10
Match: AT4G11070.1 (WRKY family transcription factor )

HSP 1 Score: 131.0 bits (328), Expect = 1.6e-30
Identity = 86/222 (38.74%), Postives = 120/222 (54.05%), Query Frame = 0

Query: 5   TLIQELNQGKELANQLRNHLHPSSSSSSSDGIL-----LIDKILRSYENALLALADGGVS 64
           +L+ EL  G + A QL+    PS S+SSS         L+  I+ S++ A+L L +G  +
Sbjct: 10  SLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILML-NGSTT 69

Query: 65  AKS---ALAP-------------------------VNAAVKD-RVVSKKRKVMAKWSEQV 124
             +    LAP                          N   K+  + SKKRK++ KW+EQV
Sbjct: 70  QHNPTIELAPDPLAHPGKVPGSPASITGNPRSEEFFNVRSKEFNLSSKKRKMLPKWTEQV 129

Query: 125 KVSSDSANEGPRCDGFSWRKYGQKDILGSNFPRGYFRCSHRFTQGCLATKQVQKSDNDPT 184
           ++S +   EGP  D FSWRKYGQKDILG+ FPR Y+RC+ R TQ C ATKQVQ+SD DPT
Sbjct: 130 RISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQRSDGDPT 189

Query: 185 VYDITYKGRHTCNRAHNSNKTPQKDQNSLLQHPMPPKQEQKP 193
           ++++TY+G HTC++                  P+P K+E KP
Sbjct: 190 IFEVTYRGTHTCSQG----------------IPLPEKRETKP 214

BLAST of Lsi02G020350 vs. TAIR 10
Match: AT4G11070.2 (WRKY family transcription factor )

HSP 1 Score: 129.4 bits (324), Expect = 4.8e-30
Identity = 61/116 (52.59%), Postives = 80/116 (68.97%), Query Frame = 0

Query: 77  SKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGSNFPRGYFRCSHRFTQGC 136
           SKKRK++ KW+EQV++S +   EGP  D FSWRKYGQKDILG+ FPR Y+RC+ R TQ C
Sbjct: 83  SKKRKMLPKWTEQVRISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYC 142

Query: 137 LATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKTPQKDQNSLLQHPMPPKQEQKP 193
            ATKQVQ+SD DPT++++TY+G HTC++                  P+P K+E KP
Sbjct: 143 WATKQVQRSDGDPTIFEVTYRGTHTCSQG----------------IPLPEKRETKP 182

BLAST of Lsi02G020350 vs. TAIR 10
Match: AT5G24110.1 (WRKY DNA-binding protein 30 )

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-26
Identity = 68/171 (39.77%), Postives = 104/171 (60.82%), Query Frame = 0

Query: 11  NQGKELANQLRNHLHPSSSSSSSD-GILLIDKILRSYENALLAL---------ADGGVSA 70
           N+  EL  + R++ H   S+SS +    L  KIL+SY  +L  +         + GG S 
Sbjct: 15  NEINELMIEGRDYAHQFGSASSQETREHLAKKILQSYHKSLTIMNYSGELDQVSQGGGSP 74

Query: 71  KSALAPVNAAVKDRVVSKKRKVMAKWSEQVKVSSDSANEGPRCDGFSWRKYGQKDILGSN 130
           KS     ++  +  V+   +K M +WS +V+++  +  +    DGFSWRKYGQKDILG+ 
Sbjct: 75  KSD----DSDQEPLVIKSSKKSMPRWSSKVRIAPGAGVDRTLDDGFSWRKYGQKDILGAK 134

Query: 131 FPRGYFRCSHRFTQGCLATKQVQKSDNDPTVYDITYKGRHTCNRAHNSNKT 172
           FPRGY+RC++R +QGC ATKQVQ+SD +  + +I+Y+G H+C++A N   T
Sbjct: 135 FPRGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYRGIHSCSQAANVGTT 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUP63.8e-3234.47Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 ... [more]
Q9SKD91.0e-2947.73Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 ... [more]
Q8H0Y82.3e-2938.74Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 ... [more]
Q9FL622.0e-2539.77Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 ... [more]
Q0DFT76.1e-2234.31Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 P... [more]
Match NameE-valueIdentityDescription
A0A5D3C9476.7e-14183.95Putative WRKY transcription factor 53 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CGE28.8e-14183.95probable WRKY transcription factor 53 OS=Cucumis melo OX=3656 GN=LOC103500461 PE... [more]
E7CEY44.8e-13180.27WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY50 PE=2 SV=1[more]
A0A6J1DUY21.4e-10168.81probable WRKY transcription factor 46 OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A6J1JVH21.4e-9867.00probable WRKY transcription factor 46 OS=Cucurbita maxima OX=3661 GN=LOC11148869... [more]
Match NameE-valueIdentityDescription
XP_038899647.12.5e-14285.81probable WRKY transcription factor 53 [Benincasa hispida][more]
KAA0050658.11.4e-14083.95putative WRKY transcription factor 53 [Cucumis melo var. makuwa] >TYK07774.1 put... [more]
XP_008461982.11.8e-14083.95PREDICTED: probable WRKY transcription factor 53 [Cucumis melo][more]
NP_001267693.11.0e-13080.27probable WRKY transcription factor 46-like [Cucumis sativus] >ADU52527.1 WRKY pr... [more]
XP_022157612.12.8e-10168.81probable WRKY transcription factor 46 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G23810.12.7e-3334.47WRKY family transcription factor [more]
AT2G46400.17.3e-3147.73WRKY DNA-binding protein 46 [more]
AT4G11070.11.6e-3038.74WRKY family transcription factor [more]
AT4G11070.24.8e-3052.59WRKY family transcription factor [more]
AT5G24110.11.4e-2639.77WRKY DNA-binding protein 30 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 102..164
e-value: 1.1E-37
score: 141.2
IPR003657WRKY domainPFAMPF03106WRKYcoord: 104..163
e-value: 4.3E-25
score: 87.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 97..165
score: 21.539698
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 86..165
e-value: 4.9E-26
score: 92.3
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 101..164
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..184
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..192
NoneNo IPR availablePANTHERPTHR32096:SF102WRKY TRANSCRIPTION FACTOR 46-RELATEDcoord: 2..292
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 2..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G020350.1Lsi02G020350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042742 defense response to bacterium
biological_process GO:0048527 lateral root development
biological_process GO:1900457 regulation of brassinosteroid mediated signaling pathway
biological_process GO:2000022 regulation of jasmonic acid mediated signaling pathway
biological_process GO:2000070 regulation of response to water deprivation
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0009751 response to salicylic acid
cellular_component GO:0005634 nucleus
molecular_function GO:0000987 cis-regulatory region sequence-specific DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding