Lsi02G017370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G017370
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPurple acid phosphatase
Locationchr02: 23086852 .. 23089253 (+)
RNA-Seq ExpressionLsi02G017370
SyntenyLsi02G017370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAATCTCATCCCTCTCATTCTTCTCATCTCCTTCCTCCCCATTTCAATCCAATCCAAGGTCTCCATTTCTTTCTCCCCTTCCATTCTCTCCAAATCCGGCGATCCAGTTCAAATCCAATGGTCTGGAATCGAATCCCCTTCCAAGCTCGATTGGCTCGGAATTTACTCGCCCCCAAATTCCTCCCATAAGCATTTCATCGGCTACGTCTTCCTTTCTTCCTCTCCCACCTGGCAATCCGGCTCTGGATCCGTTTCCATCCCCTTAGTCAACCTCCGATCCAATTACTCCTTCAGGATTTTCCGGTGGACCGAATCGGAGATCGACGACAAGCATCATGACCACGACCACAACCCTCTTCCCGGCACGGCCCACCTTCTCGCGGCCTCGGAGGAGCTCCGATTCTCCACCGGCAGCGGCCCGGAGCAGATCCATCTGGCGTTCACGGATCACGATGACGAAATGAGAGTGATGTTCGTGACGGAGGATGGATCGGAAAGGTATGTGAGATATGGGGAAAAGAAGGAGAAGCTTGATCAGATTGTTGTGGCGCGTGTTGAGAGGTATGAGAGGGAGCATATGTGCGATTCCCCTGCTAATGACAGCATTGGCTGGAGAGATCCTGGTTTTATTCATGATGCTGTCATGAATAAATTGCAGAAAGGAGCAAAAGTTTATTATCAGGTAATTTTGTTTTTTCTTTTGCCTAATTTGGGATTCTGGTGGAATTTGTGATGCATTTGCTTTTGAGTTTTGAACATTGGCCTTGGTAGTTCAGCAGTTCATCAAATGGTTTTTTAGGGTATTTGTTTGGTGAAGATTTATGCAGCTTGTTTTAAATGTTGATTCATTTTTGTACTCTGCTCTGGTATAGCCATGAGGAATCATGGAAGACATTGGTACTATATGATGGTTCTATTCTAGTACATGAATTGCTATTGGTTGACTAATTAAGATTTGAAATGTTAAAGGCCTGATTTCATTCATGGATTCACTCAAATAAGAAAGGAGATTGATGGTGCTTTTTTCATTTTTGTATTGAGTTGACTTATAGTCTAGACCATCTAGGTACAGAACTATAATGCAATGCATTTTGCTTTTACCAGGTTGGAAGTGATTCTAAAGGTTGGAGTAGTATTCTAAACTTCGTGTCACGTAATGAGGACTCGGATGAAACGATTGCTTTCCTTTTCGGTGATATGGGAGCTGCAACACCATACATGACCTTTGTCCGAACACAGGAGGAAAGCATATCTACTGTAAGGTGGATACTTCGCGACATTGAAGCGCTTGGTGACAAACCTGCATTTATCTCTCACATTGGAGATATCAGCTATGCAAGGGGTCATGCGTGGTTATGGGACGTTTTCTTCAATCAGATCGAACCTGTGGCATCAAAAGTTGCATACCATGTATGTATTGGCAATCATGAATATGACTGGCCTGTTCAACCGTGGAAACCCGAGTGGGCATTCGGGATATATGGGAAAGATGGGGGAGGGGAATGTGGGGTGCCATATAGTTTGAAGTTCAACATGCCAGGAAACTCTTCAGAACCTACCGAAAGCCACAGCTTGCCTACTCGAAACCTTTTCTACTCTTTTAACATGGGATCGGTGCATTTTGTGTACATATCAACAGAAACCAATTTCCTTCAAGGAAGTAGCCAATATGAATTCATTAAGCGTGATTTGGAGTCCGTTGATCGGAAGAAGACACCATTCGTAGTGGTACAGGGCCACAGACCAATGTATACAACGAGCAACGAACTTAGAGATGCCCCATTGAGGGAGAAAATGCTGCACCACTTGGAACCATTGTTTGTGAAAAATAATGTGACATTGGCATTATGGGGTCATGTCCATAGATATGAAAGGTTTTGCCCGCTTAATAACTACACTTGTGGAAGTATGGGTGTGGATGGGGAAGGCTGGGAGGCATTACCCGTCCATCTCGTCATCGGAATGGCTGGACAAGATTGGCAACCAATCTGGGAGCCGAGACCAAACCACCCAGATGACCCGATCTTCCCACAACCAAAGCGGTCGATGTACCGTGGAGGCGAATTCGGTTACACCAGATTGTTTGCTACAAAAGAGAAGCTTACAATCTCTTATGTTGGAAATCATGATGGAGAGGTACACGATTCTGTCGAGATTTTGGCGTCGGGACAAGTTCTAAATGGTGGAGTTGGTGCAAAATTCAGCAACAGTAGTATTGCAAACTCGACGACCAGGAACGAGATGCTAGAATTTTCATTCTCCTGGTATGTGATGGGAGCAAGTATTTTGGTTCTTGGAGCCTTCGTTGGATACATTATTGGATTTGTTTCACATGCGAGGAAGAATTCAATATCAAGAAACAACTGGACTCCTGTGAAGACTGAAGAGTTGCAATGA

mRNA sequence

ATGAATAATCTCATCCCTCTCATTCTTCTCATCTCCTTCCTCCCCATTTCAATCCAATCCAAGGTCTCCATTTCTTTCTCCCCTTCCATTCTCTCCAAATCCGGCGATCCAGTTCAAATCCAATGGTCTGGAATCGAATCCCCTTCCAAGCTCGATTGGCTCGGAATTTACTCGCCCCCAAATTCCTCCCATAAGCATTTCATCGGCTACGTCTTCCTTTCTTCCTCTCCCACCTGGCAATCCGGCTCTGGATCCGTTTCCATCCCCTTAGTCAACCTCCGATCCAATTACTCCTTCAGGATTTTCCGGTGGACCGAATCGGAGATCGACGACAAGCATCATGACCACGACCACAACCCTCTTCCCGGCACGGCCCACCTTCTCGCGGCCTCGGAGGAGCTCCGATTCTCCACCGGCAGCGGCCCGGAGCAGATCCATCTGGCGTTCACGGATCACGATGACGAAATGAGAGTGATGTTCGTGACGGAGGATGGATCGGAAAGGTATGTGAGATATGGGGAAAAGAAGGAGAAGCTTGATCAGATTGTTGTGGCGCGTGTTGAGAGGTATGAGAGGGAGCATATGTGCGATTCCCCTGCTAATGACAGCATTGGCTGGAGAGATCCTGGTTTTATTCATGATGCTGTCATGAATAAATTGCAGAAAGGAGCAAAAGTTTATTATCAGGTTGGAAGTGATTCTAAAGGTTGGAGTAGTATTCTAAACTTCGTGTCACGTAATGAGGACTCGGATGAAACGATTGCTTTCCTTTTCGGTGATATGGGAGCTGCAACACCATACATGACCTTTGTCCGAACACAGGAGGAAAGCATATCTACTGTAAGGTGGATACTTCGCGACATTGAAGCGCTTGGTGACAAACCTGCATTTATCTCTCACATTGGAGATATCAGCTATGCAAGGGGTCATGCGTGGTTATGGGACGTTTTCTTCAATCAGATCGAACCTGTGGCATCAAAAGTTGCATACCATGTATGTATTGGCAATCATGAATATGACTGGCCTGTTCAACCGTGGAAACCCGAGTGGGCATTCGGGATATATGGGAAAGATGGGGGAGGGGAATGTGGGGTGCCATATAGTTTGAAGTTCAACATGCCAGGAAACTCTTCAGAACCTACCGAAAGCCACAGCTTGCCTACTCGAAACCTTTTCTACTCTTTTAACATGGGATCGGTGCATTTTGTGTACATATCAACAGAAACCAATTTCCTTCAAGGAAGTAGCCAATATGAATTCATTAAGCGTGATTTGGAGTCCGTTGATCGGAAGAAGACACCATTCGTAGTGGTACAGGGCCACAGACCAATGTATACAACGAGCAACGAACTTAGAGATGCCCCATTGAGGGAGAAAATGCTGCACCACTTGGAACCATTGTTTGTGAAAAATAATGTGACATTGGCATTATGGGGTCATGTCCATAGATATGAAAGGTTTTGCCCGCTTAATAACTACACTTGTGGAAGTATGGGTGTGGATGGGGAAGGCTGGGAGGCATTACCCGTCCATCTCGTCATCGGAATGGCTGGACAAGATTGGCAACCAATCTGGGAGCCGAGACCAAACCACCCAGATGACCCGATCTTCCCACAACCAAAGCGGTCGATGTACCGTGGAGGCGAATTCGGTTACACCAGATTGTTTGCTACAAAAGAGAAGCTTACAATCTCTTATGTTGGAAATCATGATGGAGAGGTACACGATTCTGTCGAGATTTTGGCGTCGGGACAAGTTCTAAATGGTGGAGTTGGTGCAAAATTCAGCAACAGTAGTATTGCAAACTCGACGACCAGGAACGAGATGCTAGAATTTTCATTCTCCTGGTATGTGATGGGAGCAAGTATTTTGGTTCTTGGAGCCTTCGTTGGATACATTATTGGATTTGTTTCACATGCGAGGAAGAATTCAATATCAAGAAACAACTGGACTCCTGTGAAGACTGAAGAGTTGCAATGA

Coding sequence (CDS)

ATGAATAATCTCATCCCTCTCATTCTTCTCATCTCCTTCCTCCCCATTTCAATCCAATCCAAGGTCTCCATTTCTTTCTCCCCTTCCATTCTCTCCAAATCCGGCGATCCAGTTCAAATCCAATGGTCTGGAATCGAATCCCCTTCCAAGCTCGATTGGCTCGGAATTTACTCGCCCCCAAATTCCTCCCATAAGCATTTCATCGGCTACGTCTTCCTTTCTTCCTCTCCCACCTGGCAATCCGGCTCTGGATCCGTTTCCATCCCCTTAGTCAACCTCCGATCCAATTACTCCTTCAGGATTTTCCGGTGGACCGAATCGGAGATCGACGACAAGCATCATGACCACGACCACAACCCTCTTCCCGGCACGGCCCACCTTCTCGCGGCCTCGGAGGAGCTCCGATTCTCCACCGGCAGCGGCCCGGAGCAGATCCATCTGGCGTTCACGGATCACGATGACGAAATGAGAGTGATGTTCGTGACGGAGGATGGATCGGAAAGGTATGTGAGATATGGGGAAAAGAAGGAGAAGCTTGATCAGATTGTTGTGGCGCGTGTTGAGAGGTATGAGAGGGAGCATATGTGCGATTCCCCTGCTAATGACAGCATTGGCTGGAGAGATCCTGGTTTTATTCATGATGCTGTCATGAATAAATTGCAGAAAGGAGCAAAAGTTTATTATCAGGTTGGAAGTGATTCTAAAGGTTGGAGTAGTATTCTAAACTTCGTGTCACGTAATGAGGACTCGGATGAAACGATTGCTTTCCTTTTCGGTGATATGGGAGCTGCAACACCATACATGACCTTTGTCCGAACACAGGAGGAAAGCATATCTACTGTAAGGTGGATACTTCGCGACATTGAAGCGCTTGGTGACAAACCTGCATTTATCTCTCACATTGGAGATATCAGCTATGCAAGGGGTCATGCGTGGTTATGGGACGTTTTCTTCAATCAGATCGAACCTGTGGCATCAAAAGTTGCATACCATGTATGTATTGGCAATCATGAATATGACTGGCCTGTTCAACCGTGGAAACCCGAGTGGGCATTCGGGATATATGGGAAAGATGGGGGAGGGGAATGTGGGGTGCCATATAGTTTGAAGTTCAACATGCCAGGAAACTCTTCAGAACCTACCGAAAGCCACAGCTTGCCTACTCGAAACCTTTTCTACTCTTTTAACATGGGATCGGTGCATTTTGTGTACATATCAACAGAAACCAATTTCCTTCAAGGAAGTAGCCAATATGAATTCATTAAGCGTGATTTGGAGTCCGTTGATCGGAAGAAGACACCATTCGTAGTGGTACAGGGCCACAGACCAATGTATACAACGAGCAACGAACTTAGAGATGCCCCATTGAGGGAGAAAATGCTGCACCACTTGGAACCATTGTTTGTGAAAAATAATGTGACATTGGCATTATGGGGTCATGTCCATAGATATGAAAGGTTTTGCCCGCTTAATAACTACACTTGTGGAAGTATGGGTGTGGATGGGGAAGGCTGGGAGGCATTACCCGTCCATCTCGTCATCGGAATGGCTGGACAAGATTGGCAACCAATCTGGGAGCCGAGACCAAACCACCCAGATGACCCGATCTTCCCACAACCAAAGCGGTCGATGTACCGTGGAGGCGAATTCGGTTACACCAGATTGTTTGCTACAAAAGAGAAGCTTACAATCTCTTATGTTGGAAATCATGATGGAGAGGTACACGATTCTGTCGAGATTTTGGCGTCGGGACAAGTTCTAAATGGTGGAGTTGGTGCAAAATTCAGCAACAGTAGTATTGCAAACTCGACGACCAGGAACGAGATGCTAGAATTTTCATTCTCCTGGTATGTGATGGGAGCAAGTATTTTGGTTCTTGGAGCCTTCGTTGGATACATTATTGGATTTGTTTCACATGCGAGGAAGAATTCAATATCAAGAAACAACTGGACTCCTGTGAAGACTGAAGAGTTGCAATGA

Protein sequence

MNNLIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSSHKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNFVSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSIANSTTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ
Homology
BLAST of Lsi02G017370 vs. ExPASy Swiss-Prot
Match: Q9LMG7 (Probable inactive purple acid phosphatase 2 OS=Arabidopsis thaliana OX=3702 GN=PAP2 PE=2 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 2.2e-274
Identity = 447/654 (68.35%), Postives = 526/654 (80.43%), Query Frame = 0

Query: 7   LILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSSHKH 66
           L+L +S    S  SK +IS SP+ L++SGD V IQWSG++SPS LDWLG+YSPP S + H
Sbjct: 9   LLLFVSVFVSSADSKATISISPNALNRSGDSVVIQWSGVDSPSDLDWLGLYSPPESPNDH 68

Query: 67  FIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPGTAH 126
           FIGY FL+ S TW+ G GS+S+PL NLRSNY+FRIFRW+ESEID KH DHD NPLPGT H
Sbjct: 69  FIGYKFLNESSTWKDGFGSISLPLTNLRSNYTFRIFRWSESEIDPKHKDHDQNPLPGTKH 128

Query: 127 LLAASEELRFSTGSG-PEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIVVA 186
           LLA SE+L F +G G PEQIHL+FT+  + MRVMFV  DG ER+VRYGE K+ L     A
Sbjct: 129 LLAESEQLTFGSGVGMPEQIHLSFTNMVNTMRVMFVAGDGEERFVRYGESKDLLGNSAAA 188

Query: 187 RVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNFVS 246
           R  RYEREHMCDSPAN +IGWRDPG+I D VM  L  G + YYQVGSDSKGWS I ++++
Sbjct: 189 RGMRYEREHMCDSPANSTIGWRDPGWIFDTVMKNLNDGVRYYYQVGSDSKGWSEIHSYIA 248

Query: 247 RNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISHIGDIS 306
           R+  ++ET+AF+FGDMG ATPY TF+RTQ+ESISTV+WILRDIEALGDKPA ISHIGDIS
Sbjct: 249 RDVTAEETVAFMFGDMGCATPYTTFIRTQDESISTVKWILRDIEALGDKPAMISHIGDIS 308

Query: 307 YARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGECGV 366
           YARG++W+WD FF Q+EP+AS V YHVCIGNHEYD+  QPWKP+WA  IYG DGGGECGV
Sbjct: 309 YARGYSWVWDEFFAQVEPIASTVPYHVCIGNHEYDFSTQPWKPDWAASIYGNDGGGECGV 368

Query: 367 PYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKRDL 426
           PYSLKFNMPGNSSE T   + PTRNL+YS++MG+VHFVYISTETNFL+G SQYEFIKRDL
Sbjct: 369 PYSLKFNMPGNSSESTGMKAPPTRNLYYSYDMGTVHFVYISTETNFLKGGSQYEFIKRDL 428

Query: 427 ESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHRYE 486
           ESVDRKKTPFVVVQGHRPMYTTSNE+RD  +R+KM+ HLEPLFVKNNVTLALWGHVHRYE
Sbjct: 429 ESVDRKKTPFVVVQGHRPMYTTSNEVRDTMIRQKMVEHLEPLFVKNNVTLALWGHVHRYE 488

Query: 487 RFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMY 546
           RFCP++N TCG+       W+  PVHLVIGMAGQDWQPIW+PRPNHPD PIFPQP++SMY
Sbjct: 489 RFCPISNNTCGTQ------WQGNPVHLVIGMAGQDWQPIWQPRPNHPDLPIFPQPEQSMY 548

Query: 547 RGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGG-VGAKFSN-SSIANS 606
           R GEFGYTRL A KEKLT+S+VGNHDGEVHD+VE+LASG V++G     K  N  ++  S
Sbjct: 549 RTGEFGYTRLVANKEKLTVSFVGNHDGEVHDTVEMLASGVVISGSKESTKIPNLKTVPAS 608

Query: 607 TTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEE 658
            T     E +  WY  GA ++V+G  +G+IIGF +  +K+S S N W PVK EE
Sbjct: 609 ATLMGKSESNALWYAKGAGLMVVGVLLGFIIGFFTRGKKSS-SGNRWIPVKNEE 655

BLAST of Lsi02G017370 vs. ExPASy Swiss-Prot
Match: Q9ZQ81 (Probable inactive purple acid phosphatase 9 OS=Arabidopsis thaliana OX=3702 GN=PAP9 PE=2 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 2.3e-263
Identity = 437/656 (66.62%), Postives = 517/656 (78.81%), Query Frame = 0

Query: 9   LLISFLPI-SIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSSHKHF 68
           L   FL I S+ SK +IS SP  L++SGD V I+WSG+ESPS LDWLGIYSPP+S H HF
Sbjct: 8   LFFFFLLISSVYSKATISISPQTLNRSGDIVVIKWSGVESPSDLDWLGIYSPPDSPHDHF 67

Query: 69  IGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPGTAHL 128
           IGY FLS SPTWQSGSGS+S+PL NLRSNY+FRIF WT+SEI+ KH DHDHNPLPGT HL
Sbjct: 68  IGYKFLSDSPTWQSGSGSISLPLTNLRSNYTFRIFHWTQSEINPKHQDHDHNPLPGTRHL 127

Query: 129 LAASEELRFSTG-SGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIVVAR 188
           L  S +L F    + PEQIHL++TD+ +EMRV+FVT DG ER  RYGE K+KLD I VAR
Sbjct: 128 LTESNQLNFRFAVNRPEQIHLSYTDNINEMRVVFVTGDGEEREARYGEVKDKLDNIAVAR 187

Query: 189 VERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNFVSR 248
             RYE EHMC +PAN ++GWRDPG+  DAVM  L++G + YYQVGSD KGWS I +FVSR
Sbjct: 188 GVRYEIEHMCHAPANSTVGWRDPGWTFDAVMKNLKQGIRYYYQVGSDLKGWSEIHSFVSR 247

Query: 249 NEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALG-DKPAFISHIGDIS 308
           NE S+ET+AF+FGDMG  TPY TF+R +EES+STV+WILRDIEALG DKP  +SHIGDIS
Sbjct: 248 NEGSEETLAFMFGDMGCYTPYTTFIRGEEESLSTVKWILRDIEALGDDKPVIVSHIGDIS 307

Query: 309 YARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGECGV 368
           YARG++W+WD FF QIEP+ASKV YHVCIGNHEYDWP QPWKP+WA  +YGKD GGECGV
Sbjct: 308 YARGYSWIWDEFFTQIEPIASKVPYHVCIGNHEYDWPNQPWKPDWAAYVYGKDSGGECGV 367

Query: 369 PYSLKFNMPGNSSEPTESHSLP-TRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKRD 428
           PYS+KFNMPGNS+E T     P +RNL+YS++MGSVHFVYISTET+FL+G  QY F+K D
Sbjct: 368 PYSVKFNMPGNSTEATGMVKGPQSRNLYYSYDMGSVHFVYISTETDFLKGGKQYSFLKSD 427

Query: 429 LESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHRY 488
           LESV+R KTPFVVVQGHRPMYTTS ++RDA +REKM+ HLEPL VKNNVT+ALWGHVHRY
Sbjct: 428 LESVNRSKTPFVVVQGHRPMYTTSRKIRDAAIREKMIEHLEPLLVKNNVTVALWGHVHRY 487

Query: 489 ERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSM 548
           ERFC ++N TC      GE W+  PVHLVIGMAG+D QP+WEPR NH D PIFPQP  SM
Sbjct: 488 ERFCAISNNTC------GERWQGNPVHLVIGMAGKDSQPMWEPRANHEDVPIFPQPANSM 547

Query: 549 YRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSIANST 608
           YRGGEFGY RL A KE+LT+SYVGNHDGEVHD VEILASG+V++G      S+    +S 
Sbjct: 548 YRGGEFGYIRLVANKERLTLSYVGNHDGEVHDVVEILASGEVISG------SDDGTKDSN 607

Query: 609 TRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNS---ISRNNWTPVKTEE 658
             +E  +F+  WY+ GAS++V+G   GY +GF+S  +K S    S  +W  VK EE
Sbjct: 608 FGSES-DFAVLWYIEGASVMVVGVIFGYFVGFLSRKKKESGVGSSNRSWIQVKNEE 650

BLAST of Lsi02G017370 vs. ExPASy Swiss-Prot
Match: Q9LMX4 (Probable inactive purple acid phosphatase 1 OS=Arabidopsis thaliana OX=3702 GN=PAP1 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.8e-58
Identity = 180/609 (29.56%), Postives = 279/609 (45.81%), Query Frame = 0

Query: 14  LPISIQSKVSISFSPSILSKSG---DPVQIQWSGIESPSKLDWLGIYSPPNSSHKHFIG- 73
           +   +  K  +  SP++L  +G   + V +Q+S    PS  DW+G++SP + +     G 
Sbjct: 38  ITFGLNEKAYVKASPTVLGSNGQHSELVLVQYSS-PKPSDDDWIGVFSPADFNASTCPGD 97

Query: 74  -----------------YVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDK 133
                            Y   S+     +G+GS+ + L+N RS++SF +F  +   ++ K
Sbjct: 98  NKMVQPPRLCSAPVKFQYANFSNPRYTNTGTGSLKLQLINQRSDFSFALF--SGGLLNPK 157

Query: 134 HHDHDHNPLPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDG---SERY 193
                         L+A S ++ F   + P    LA     DEM V + +  G   +E  
Sbjct: 158 --------------LVAISNKVAFENPNAPVYPRLALGKEWDEMTVTWTSGYGLNLAEPV 217

Query: 194 VRYGEKKEKLDQIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQ 253
           V +G K  +  ++  A    + R  MC +PA  ++GWRDPG+IH A + +L   +K  Y+
Sbjct: 218 VEWGVKGGE-RKLSPAGTLTFARNSMCGAPAR-TVGWRDPGYIHTAFLKELWPNSKYTYR 277

Query: 254 VGSD-SKG---WSSILNFVSRNEDSDETI--AFLFGDMGAA-----TPYMTFVRTQEESI 313
           VG   S G   WS    F S       ++    +FGDMG A     + Y  F   Q  S+
Sbjct: 278 VGHRLSNGALIWSKEYQFKSSPFPGQNSVQQVVIFGDMGKAEVDGSSEYNDF---QRASL 337

Query: 314 STVRWILRDIEALGDKPAFISHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHE 373
           +T + +++D++    K   + HIGDI YA G+   WD F  QIEP+AS V Y +  GNHE
Sbjct: 338 NTTKQLIKDLK----KTDAVFHIGDICYANGYLSQWDQFIAQIEPIASTVPYMIASGNHE 397

Query: 374 YDWPVQPWKPEWAFGIYGKDGGGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMG 433
             WP      E      G D GGECGVP    F +P  +             ++YS + G
Sbjct: 398 RVWPNSGSFYE------GLDSGGECGVPAETMFYVPAQN----------RAKVWYSSDYG 457

Query: 434 SVHFVYISTETNFLQGSSQYEFIKRDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDAPL 493
              F    TE ++ +G+ QY FI+  L SVDR+K P+++   HR +  +S      +   
Sbjct: 458 MFRFCVADTEHDWREGTEQYNFIEHCLASVDRQKQPWLIFLAHRVLGYSSTYFYAEEGSF 517

Query: 494 REKM-LHHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMGVDGEGWEAL---PVHL 553
            E M    L+ L+ K  V +A++GH H YER CP+    C S   +   ++A     +H+
Sbjct: 518 AEPMGRESLQKLWQKYKVDIAIYGHAHNYERTCPVYQSVCTSH--EKSNYKAPLNGTIHI 577

Query: 554 VIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLFA-TKEKLTISYVGNHD 581
           V G  G       + +PN            S++R  ++G+ +L A     L   Y  + D
Sbjct: 578 VAGGGGAGLAEFSDLQPNW-----------SLFRDYDYGFLKLTAIDHSNLLFEYKKSSD 591

BLAST of Lsi02G017370 vs. ExPASy Swiss-Prot
Match: Q5MAU8 (Probable inactive purple acid phosphatase 27 OS=Arabidopsis thaliana OX=3702 GN=PAP27 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 5.2e-58
Identity = 177/600 (29.50%), Postives = 271/600 (45.17%), Query Frame = 0

Query: 24  ISFSPSILSKSGDPVQIQWSGI----ESPSKLDWLGIYSPPN--------SSHKHF---- 83
           I  SP +L   G     +W  +      PS  DW+G++SP          +  K      
Sbjct: 47  IHVSPLVLGSQGQ--DTEWVNVVISNPEPSSDDWVGVFSPAKFDSSSCAPTDDKEIAPFI 106

Query: 84  ----IGYVFLSSSPTW-QSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLP 143
               + Y++  SSP + ++G+  +   L+N R+++SF +F    S           NP  
Sbjct: 107 CSAPVKYMYAKSSPDYMKTGNAVLKFMLINQRADFSFALFTGGLS-----------NPT- 166

Query: 144 GTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE--KKEKLD 203
               L++ S  + F     P    LA     DEM V + +       V + E  +K    
Sbjct: 167 ----LVSVSNHVSFINPKAPVYPRLALGKKWDEMTVTWTSGYNIGEAVPFVEWSRKGTRS 226

Query: 204 QIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSD----SKG 263
           +   A    + R  MC +PA  ++GWRDPGFIH A +  L    K  Y++G +    S  
Sbjct: 227 RRSPAGTLTFTRNSMCGAPAR-TVGWRDPGFIHTASLKDLWPNLKYTYRMGHELMNGSIV 286

Query: 264 WSSILNFVSRNEDSDETI--AFLFGDMG-----AATPYMTFVRTQEESISTVRWILRDIE 323
           WS    F S      +++    +FGDMG      +  Y  +   Q  S++T   +++D++
Sbjct: 287 WSKNFTFKSSPYPGQDSLQRVIIFGDMGKGERDGSNEYNDY---QPGSLNTTDQLIKDLK 346

Query: 324 ALGDKPAFISHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPE 383
            +      + HIGDI+YA G+   WD F  Q+EP+AS V Y V  GNHE DW      P+
Sbjct: 347 NID----IVFHIGDITYANGYISQWDQFTAQVEPIASTVPYMVASGNHERDW------PD 406

Query: 384 WAFGIYGKDGGGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTET 443
                 GKD GGECGVP    F+ P  +              +YS + G   F    TE 
Sbjct: 407 SGSFYGGKDSGGECGVPAETMFDFPAENK----------AKFWYSADYGMFRFCVADTEH 466

Query: 444 NFLQGSSQYEFIKRDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDAPLREKM-LHHLEP 503
           ++ +GS QY+FI+R L SVDR+  P+++   HR +  ++N+   ++    E M    L+ 
Sbjct: 467 DWREGSEQYQFIERCLASVDRRAQPWLIFIAHRVLGYSTNDWYGQEGSFEEPMGRESLQK 526

Query: 504 LFVKNNVTLALWGHVHRYERFCPLNNYTCGSMGVDGE-----GWEALPVHLVIGMAGQDW 563
           L+ K  V +A +GHVH YER CP+    C    +D E     G     +H+V+G AG   
Sbjct: 527 LWQKYKVDIAFYGHVHNYERTCPIYQNQC----MDNEKSHYSGAFKGTIHVVVGGAG--- 586

Query: 564 QPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLFA-TKEKLTISYVGNHDGEVHDSVEI 581
                   +H       +PK S++R  ++G+ +L A     L   Y  + +G VHDS  I
Sbjct: 587 --------SHLSSFSSLKPKWSIFRDYDYGFVKLTAFDHSSLLFEYKKSSNGAVHDSFTI 589

BLAST of Lsi02G017370 vs. ExPASy Swiss-Prot
Match: Q8H1R2 (Probable inactive purple acid phosphatase 24 OS=Arabidopsis thaliana OX=3702 GN=PAP24 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.4e-57
Identity = 171/606 (28.22%), Postives = 272/606 (44.88%), Query Frame = 0

Query: 16  ISIQSKVSISFSPSILSKSGDPVQIQWSGI----ESPSKLDWLGIYSP---------PNS 75
           +++ S V +  SP +L   G+    +W  +      P+  DW+G++SP         P S
Sbjct: 42  LALDSSVKLHASPQVLGSQGE--DTEWVNLAISNPKPTSDDWIGVFSPAKFDSGNCWPTS 101

Query: 76  SHKHF--------IGYVFLSSSPTW-QSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKH 135
             K          I Y++ +S P + +SG+ ++   ++N R++ SF +F     E     
Sbjct: 102 GGKEKTPYICSSPIKYMYCNSHPDYMKSGNVTLKFQIINQRADVSFALFSNGVQE----- 161

Query: 136 HDHDHNPLPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYG 195
                       HLL  S  + F     P    LA   + DEM V + +    +  V + 
Sbjct: 162 -----------PHLLGVSNPVAFFNPKAPVYPRLALGKNWDEMTVTWTSGYNIDEAVPFI 221

Query: 196 EKKEK--LDQIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVG 255
           E   K    +   A    + R  MC +PA   +GWRDPGF H + + +L    +  Y++G
Sbjct: 222 EWSAKGLPARRSPAGTLTFNRNSMCGNPAR-GVGWRDPGFFHTSFLKELWPNREYIYRLG 281

Query: 256 SD----SKGWSSILNFVSR---NEDSDETIAFLFGDMG-----AATPYMTFVRTQEESIS 315
            D    S  WS    FVS     +DS + +  +FGDMG      +  Y  +   Q  S++
Sbjct: 282 HDLVNGSTIWSKNYTFVSSPYPGQDSKQRV-IIFGDMGKGERDGSNEYNDY---QPGSLN 341

Query: 316 TVRWILRDIEALGDKPAFISHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEY 375
           T   +++D++ +      + HIGD++Y+ G+   WD F  Q++P+AS V Y +  GNHE 
Sbjct: 342 TTDQVIKDLKDID----IVFHIGDLTYSNGYLSQWDQFTAQVQPIASTVPYMIASGNHER 401

Query: 376 DWPVQPWKPEWAFGIYGKDGGGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGS 435
           DW      P+      G D GGECGVP    F  P  +              +Y  + G 
Sbjct: 402 DW------PDTGSFYAGTDSGGECGVPAETMFYFPAEN----------RAKFWYKTDYGM 461

Query: 436 VHFVYISTETNFLQGSSQYEFIKRDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDAPLR 495
             F    +E ++ +G+ QY+FI+  L +VDRK  P+++   HR +  ++N+   ++    
Sbjct: 462 FRFCVADSEHDWREGTEQYKFIENCLATVDRKTQPWLIFIAHRVLGYSTNDWYGKEGTFE 521

Query: 496 EKM-LHHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMGVDG-EGWEALPVHLVIG 555
           E M    L+ L+ K  V LA +GHVH YER CP+    C +   D   G     +H+V+G
Sbjct: 522 EPMGRESLQKLWQKYKVDLAFYGHVHNYERTCPIYESQCVNNDKDHYSGTFKGTIHVVVG 581

Query: 556 MAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLFAT-KEKLTISYVGNHDGEV 581
            AG    P                PK S+ R  +FG+ +L A+    L   Y  +  G+V
Sbjct: 582 GAGSHLSPFSS-----------LVPKWSLVRDYDFGFVKLTASDHSSLLFEYKKSSTGQV 593

BLAST of Lsi02G017370 vs. ExPASy TrEMBL
Match: A0A0A0KNH8 (Purple acid phosphatase OS=Cucumis sativus OX=3659 GN=Csa_5G487720 PE=3 SV=1)

HSP 1 Score: 1298.1 bits (3358), Expect = 0.0e+00
Identity = 614/656 (93.60%), Postives = 636/656 (96.95%), Query Frame = 0

Query: 4   LIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSS 63
           +IP +LL SF+PIS QSKVS+SFSPSILSKSGD V IQWSGIESPSKLDWLGIYSPPNSS
Sbjct: 5   IIPFVLLFSFIPISFQSKVSVSFSPSILSKSGDSVHIQWSGIESPSKLDWLGIYSPPNSS 64

Query: 64  HKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 123
           HKHFIGY+FLSSSPTW+SG GSVSIPLVNLRSNY+FRIFRWTESEIDDKHHDHDHNPLPG
Sbjct: 65  HKHFIGYLFLSSSPTWESGYGSVSIPLVNLRSNYAFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 124 TAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 183
           TAHLLAAS+ELRF+ G GPEQIHLAFTD DDEMRVMFVT+DGS+RYVRYGEKKEKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTKDGSKRYVRYGEKKEKLDQIV 184

Query: 184 VARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNF 243
           VA VERYEREHMCDSPANDSIGWRDPGFIHDAVMNKL+KGAKVYYQVGSDSKGWSSILNF
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 244 VSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISHIGD 303
           VSRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPA +SHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 304 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGEC 363
           ISYARGH+WLWDVFFNQ+EPVASKVAYHVCIGNHEYDWP+QPWKPEWA GIYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQVEPVASKVAYHVCIGNHEYDWPLQPWKPEWANGIYGKDGGGEC 364

Query: 364 GVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 423
           GVPYSLKFNMPGNS+EPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR
Sbjct: 365 GVPYSLKFNMPGNSTEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 424 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHR 483
           DLESVDRKKTPF+VVQGHRPMYTTSNELRDAPLREKMLHHLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFIVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 484 YERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 543
           YERFCPLNNYTCGSMG+DGE WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 544 MYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSIANS 603
           MYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKF NSSIANS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFINSSIANS 604

Query: 604 TTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
           TT N MLEFSFSWYVMG SILVLGAF+GYIIGFVSHARKNS+SRNNWTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSLSRNNWTPVKTEELQ 660

BLAST of Lsi02G017370 vs. ExPASy TrEMBL
Match: A0A1S3CPV0 (Purple acid phosphatase OS=Cucumis melo OX=3656 GN=LOC103503324 PE=3 SV=1)

HSP 1 Score: 1295.8 bits (3352), Expect = 0.0e+00
Identity = 616/656 (93.90%), Postives = 633/656 (96.49%), Query Frame = 0

Query: 4   LIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSS 63
           +IP ILL SF+P+S QSKVSIS SPSILSKSGD V IQWSGIESPS LDWLGIYSPPNSS
Sbjct: 5   IIPFILLFSFVPLSFQSKVSISLSPSILSKSGDSVHIQWSGIESPSNLDWLGIYSPPNSS 64

Query: 64  HKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 123
           HKHF+GY+FLSSSPTW+SG GSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG
Sbjct: 65  HKHFVGYLFLSSSPTWESGYGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 124 TAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 183
           TAHLLAAS+ELRF+ G GPEQIHLAFTD DDEMRVMFVTEDGSERYVRYGEKKEKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 184

Query: 184 VARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNF 243
           VA VERYEREHMCDSPANDSIGWRDPGFIHDAVMNKL+KGAKVYYQVGSDSKGWSSILNF
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 244 VSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISHIGD 303
           VSRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPA +SHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 304 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGEC 363
           ISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWP+QPWKPEWA+GIYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYGIYGKDGGGEC 364

Query: 364 GVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 423
           GVPYSLKFNMPGN SEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR
Sbjct: 365 GVPYSLKFNMPGNFSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 424 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHR 483
           DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 484 YERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 543
           YERFCPLNNYTCGSMG+DGE WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 544 MYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSIANS 603
           MYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQVLNG VGAKF +SSIANS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGNVGAKFIDSSIANS 604

Query: 604 TTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
           TT N MLEFSFSWYVMG SILVLGAF+GYIIGFVSHARKNSISRNNWTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSISRNNWTPVKTEELQ 660

BLAST of Lsi02G017370 vs. ExPASy TrEMBL
Match: A0A6J1IE32 (Purple acid phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111471840 PE=3 SV=1)

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 589/659 (89.38%), Postives = 619/659 (93.93%), Query Frame = 0

Query: 1   MNNLIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPP 60
           M NLI LILLIS  PISIQSKVSIS SPS L+KSGDPVQIQWSG+ESPS LDWLGIYSPP
Sbjct: 30  MENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESPSNLDWLGIYSPP 89

Query: 61  NSSHKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNP 120
           NSSHKHFIGYVFLSSSP W+SGSGSVSIPLVNLRS YSFRIFRWTESEIDDKHHDHD+NP
Sbjct: 90  NSSHKHFIGYVFLSSSPGWESGSGSVSIPLVNLRSKYSFRIFRWTESEIDDKHHDHDNNP 149

Query: 121 LPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLD 180
           LPGT HLLAASEELRFS G GPEQIHLAFTD DDEMRVMFVTEDGSER+VRYGEK+E+LD
Sbjct: 150 LPGTKHLLAASEELRFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSERFVRYGEKEEELD 209

Query: 181 QIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSI 240
           QIV ARV RYEREHMCDSPANDSIGWRDPGFIHDAVM KLQ G++VYYQVGS+SKGWSSI
Sbjct: 210 QIVAARVARYEREHMCDSPANDSIGWRDPGFIHDAVMTKLQNGSRVYYQVGSESKGWSSI 269

Query: 241 LNFVSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISH 300
            +F+SRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPAF+SH
Sbjct: 270 QSFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAFVSH 329

Query: 301 IGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGG 360
           IGDISYARGHAWLWDVFFNQIEPVAS+VAYHVCIGNHEYDWP+QPWKP+WA GIYGKDGG
Sbjct: 330 IGDISYARGHAWLWDVFFNQIEPVASRVAYHVCIGNHEYDWPLQPWKPDWASGIYGKDGG 389

Query: 361 GECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF 420
           GECGVPYSLKFNMPGNSSEPT+++ +PTRNLFYSFNMGSVHFVYISTETNFL+GS Q+ F
Sbjct: 390 GECGVPYSLKFNMPGNSSEPTKTNGVPTRNLFYSFNMGSVHFVYISTETNFLKGSRQFSF 449

Query: 421 IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGH 480
           ++RDLESVDRKKTPFVVVQGHRPMYTTSNE RD PLREKMLHHLEPLFVKNNVTLALWGH
Sbjct: 450 LERDLESVDRKKTPFVVVQGHRPMYTTSNERRDTPLREKMLHHLEPLFVKNNVTLALWGH 509

Query: 481 VHRYERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQP 540
           VHRYERFCPL NYTCGSMGVDGE WEALPVHLVIGMAGQDWQPIWEPRPNH D PIFPQP
Sbjct: 510 VHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHLDVPIFPQP 569

Query: 541 KRSMYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSI 600
           KRSMYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQV+NGGVGAKF N S+
Sbjct: 570 KRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVNGGVGAKF-NESV 629

Query: 601 ANSTTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
            NS +RN MLE SFSWYVMG SILVLGAF+GYI+GFVSHARK   SRNNWTPVKTEELQ
Sbjct: 630 ENSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRNNWTPVKTEELQ 684

BLAST of Lsi02G017370 vs. ExPASy TrEMBL
Match: A0A6J1GI52 (Purple acid phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111454362 PE=3 SV=1)

HSP 1 Score: 1232.6 bits (3188), Expect = 0.0e+00
Identity = 588/659 (89.23%), Postives = 619/659 (93.93%), Query Frame = 0

Query: 1   MNNLIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPP 60
           M NLI LILLIS  PISIQSKVSIS SPS L+KSGDPVQIQWSG+ESPSKLDWLGIYSPP
Sbjct: 1   MENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESPSKLDWLGIYSPP 60

Query: 61  NSSHKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNP 120
           NSSHKHFIGYVFLSSSP W+SGSGSV+IPLVNLRS YSFRIFRWTESEIDDKHHDHD+NP
Sbjct: 61  NSSHKHFIGYVFLSSSPGWESGSGSVTIPLVNLRSKYSFRIFRWTESEIDDKHHDHDNNP 120

Query: 121 LPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLD 180
           LPGT HLLA+SEELRFS G GPEQIHLAFTD DDEMRVMFVTEDGSER VRYGEK+E+LD
Sbjct: 121 LPGTKHLLASSEELRFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSERLVRYGEKEEELD 180

Query: 181 QIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSI 240
           QIV ARV RYER+HMCDSPANDSIGWRDPGFIHDAVM  LQ G++VYYQVGS+SKGWSSI
Sbjct: 181 QIVAARVARYERKHMCDSPANDSIGWRDPGFIHDAVMTNLQNGSRVYYQVGSESKGWSSI 240

Query: 241 LNFVSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISH 300
            NF+SRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPAF+SH
Sbjct: 241 QNFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAFVSH 300

Query: 301 IGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGG 360
           IGDISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWP+QPWKP+WA GIYGKDGG
Sbjct: 301 IGDISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPDWASGIYGKDGG 360

Query: 361 GECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF 420
           GECGVPYSLKFNMPGNSSEPT+++ +PTRNLFYSFNMGSVHFVYISTETNFL+GS Q+ F
Sbjct: 361 GECGVPYSLKFNMPGNSSEPTKANGVPTRNLFYSFNMGSVHFVYISTETNFLKGSRQFSF 420

Query: 421 IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGH 480
           ++RDLESVDRKKTPFVVVQGHRPMYTTSNELRD PLREKMLHHLEPLFVKNNVTLALWGH
Sbjct: 421 LERDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLHHLEPLFVKNNVTLALWGH 480

Query: 481 VHRYERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQP 540
           VHRYERFCPL NYTCGSMGVDGE WEALPVHLVIGMAGQDWQPIWEPRPNH D PIFPQP
Sbjct: 481 VHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHLDVPIFPQP 540

Query: 541 KRSMYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSI 600
           KRSMYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQV+NGGVGAKF N S+
Sbjct: 541 KRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVNGGVGAKF-NESV 600

Query: 601 ANSTTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
            NS +RN MLE SFSWYVMG SILVLGAF+GYI+GFVSHARK   SRNNWTPVKTEELQ
Sbjct: 601 KNSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRNNWTPVKTEELQ 655

BLAST of Lsi02G017370 vs. ExPASy TrEMBL
Match: A0A6J1D9S2 (Purple acid phosphatase OS=Momordica charantia OX=3673 GN=LOC111018532 PE=3 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 552/660 (83.64%), Postives = 592/660 (89.70%), Query Frame = 0

Query: 1   MNNLIPLILL-ISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSP 60
           M NL  L+LL ISFLP S  SKV IS SPS LS+SGDPV I WSGIESPSKLDWLGIYSP
Sbjct: 1   MKNLFHLVLLIISFLPFSTPSKVEISLSPSTLSRSGDPVLIHWSGIESPSKLDWLGIYSP 60

Query: 61  PNSSHKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHN 120
            NSSHKHFIGYVFLSSSP W+SG+GS  +PLV+LRS+YSFRIFRWTESEID KH DHD N
Sbjct: 61  ANSSHKHFIGYVFLSSSPGWESGAGSTVLPLVDLRSSYSFRIFRWTESEIDHKHQDHDQN 120

Query: 121 PLPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKL 180
           PLPGTAHLLA SEELRFS G GP+QIHLAFTD+DDEMRVMFVTEDGSER+VRYGE KE+L
Sbjct: 121 PLPGTAHLLATSEELRFSPGRGPDQIHLAFTDNDDEMRVMFVTEDGSERFVRYGETKEQL 180

Query: 181 DQIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSS 240
           DQ+  ARV RYEREH+CDSPANDSIGWRDPGFIHDAVM  L KGA+VYYQVGSDSKGWS 
Sbjct: 181 DQVAAARVGRYEREHLCDSPANDSIGWRDPGFIHDAVMKNLPKGARVYYQVGSDSKGWSD 240

Query: 241 ILNFVSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFIS 300
             +FVSRNEDSDET+AFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPAF+S
Sbjct: 241 TKSFVSRNEDSDETLAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAFVS 300

Query: 301 HIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDG 360
           HIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWP+QPWKP+WA GIYGKDG
Sbjct: 301 HIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPDWASGIYGKDG 360

Query: 361 GGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYE 420
           GGECGVPYSL+FNMPGNSSE T ++SLPTRNLFYSFNMGSVHFVYISTETNFL+GS QYE
Sbjct: 361 GGECGVPYSLRFNMPGNSSERTGTNSLPTRNLFYSFNMGSVHFVYISTETNFLKGSKQYE 420

Query: 421 FIKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWG 480
           F+KRDLESVDRKKTPFV+VQGHRPMYTTSNELRDAPLREK+L HLEPL V+N VT+ALWG
Sbjct: 421 FLKRDLESVDRKKTPFVIVQGHRPMYTTSNELRDAPLREKLLQHLEPLLVENKVTMALWG 480

Query: 481 HVHRYERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQ 540
           HVHRYERFCPL NYTCGSM   GE +EA PVHLVIGMAGQDWQPIWEPR NHPD P+FPQ
Sbjct: 481 HVHRYERFCPLKNYTCGSM---GESFEAFPVHLVIGMAGQDWQPIWEPRLNHPDVPVFPQ 540

Query: 541 PKRSMYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSS 600
           P RSMYRGGEFGYTRL ATKEKLT+SYVGNHDGE+HDSVEILASGQVLNG          
Sbjct: 541 PTRSMYRGGEFGYTRLVATKEKLTLSYVGNHDGEIHDSVEILASGQVLNG---------- 600

Query: 601 IANSTTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
           I N+  RNE +E S SWYVMG SILVLG F+GYI+GFVSHARKNS+SRN+W+PVKTEELQ
Sbjct: 601 IENARARNETVESSVSWYVMGGSILVLGVFIGYIVGFVSHARKNSMSRNSWSPVKTEELQ 647

BLAST of Lsi02G017370 vs. NCBI nr
Match: XP_038890179.1 (probable inactive purple acid phosphatase 2 [Benincasa hispida])

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 629/659 (95.45%), Postives = 640/659 (97.12%), Query Frame = 0

Query: 1   MNNLIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPP 60
           MNNLIPLILLISF PISIQSKVSIS SPSILSKSGD VQIQWSGIESPSKLDWLGIYSPP
Sbjct: 1   MNNLIPLILLISFFPISIQSKVSISVSPSILSKSGDSVQIQWSGIESPSKLDWLGIYSPP 60

Query: 61  NSSHKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNP 120
           NSSHKHFIGYVFLSSSP+WQSGSGSVSIPLVNLRSNYSFRIFRWTESEID KHHDHDHNP
Sbjct: 61  NSSHKHFIGYVFLSSSPSWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDHKHHDHDHNP 120

Query: 121 LPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLD 180
           LPGTAHLLAAS ELRFS G GPEQIHLAFTD+DDEMRVMFVTEDGSERYVRYGE KEKLD
Sbjct: 121 LPGTAHLLAASGELRFSPGGGPEQIHLAFTDYDDEMRVMFVTEDGSERYVRYGENKEKLD 180

Query: 181 QIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSI 240
           QIV ARVERYEREHMCDSPANDSIGWRDPGFIHDAVM KLQKGAKVYYQVGSDSKGWS I
Sbjct: 181 QIVAARVERYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGAKVYYQVGSDSKGWSRI 240

Query: 241 LNFVSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISH 300
           LNFVSRNEDSDETIAFLFGDMGAATPY TFVRTQEESISTVRWILRDIEALGDKPAFISH
Sbjct: 241 LNFVSRNEDSDETIAFLFGDMGAATPYTTFVRTQEESISTVRWILRDIEALGDKPAFISH 300

Query: 301 IGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGG 360
           IGDISYARGHAWLWDVFF+QIEPVASKVAYHVCIGNHEYDWP+QPWKPEWA GIYGKDGG
Sbjct: 301 IGDISYARGHAWLWDVFFHQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWANGIYGKDGG 360

Query: 361 GECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF 420
           GECGVPYS+KFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF
Sbjct: 361 GECGVPYSMKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF 420

Query: 421 IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGH 480
           IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRD PLREKMLHHLEPLFVKNNVTLALWGH
Sbjct: 421 IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLHHLEPLFVKNNVTLALWGH 480

Query: 481 VHRYERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQP 540
           VHRYERFCPLNNYTCGSMGVDGE WEALPVHLVIGMAGQDWQPIWEPRPNHPDDP+FPQP
Sbjct: 481 VHRYERFCPLNNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPVFPQP 540

Query: 541 KRSMYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSI 600
           KRSMYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVG+KF NSSI
Sbjct: 541 KRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGSKFINSSI 600

Query: 601 ANSTTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
            NST+RNEMLE SFSW+VMGAS+LVLGAF+GYIIGFVSHARKNSISRNNWTPVKTEELQ
Sbjct: 601 TNSTSRNEMLESSFSWFVMGASLLVLGAFIGYIIGFVSHARKNSISRNNWTPVKTEELQ 659

BLAST of Lsi02G017370 vs. NCBI nr
Match: XP_004143791.1 (probable inactive purple acid phosphatase 2 [Cucumis sativus] >KGN51195.1 hypothetical protein Csa_007744 [Cucumis sativus])

HSP 1 Score: 1298.1 bits (3358), Expect = 0.0e+00
Identity = 614/656 (93.60%), Postives = 636/656 (96.95%), Query Frame = 0

Query: 4   LIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSS 63
           +IP +LL SF+PIS QSKVS+SFSPSILSKSGD V IQWSGIESPSKLDWLGIYSPPNSS
Sbjct: 5   IIPFVLLFSFIPISFQSKVSVSFSPSILSKSGDSVHIQWSGIESPSKLDWLGIYSPPNSS 64

Query: 64  HKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 123
           HKHFIGY+FLSSSPTW+SG GSVSIPLVNLRSNY+FRIFRWTESEIDDKHHDHDHNPLPG
Sbjct: 65  HKHFIGYLFLSSSPTWESGYGSVSIPLVNLRSNYAFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 124 TAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 183
           TAHLLAAS+ELRF+ G GPEQIHLAFTD DDEMRVMFVT+DGS+RYVRYGEKKEKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTKDGSKRYVRYGEKKEKLDQIV 184

Query: 184 VARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNF 243
           VA VERYEREHMCDSPANDSIGWRDPGFIHDAVMNKL+KGAKVYYQVGSDSKGWSSILNF
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 244 VSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISHIGD 303
           VSRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPA +SHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 304 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGEC 363
           ISYARGH+WLWDVFFNQ+EPVASKVAYHVCIGNHEYDWP+QPWKPEWA GIYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQVEPVASKVAYHVCIGNHEYDWPLQPWKPEWANGIYGKDGGGEC 364

Query: 364 GVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 423
           GVPYSLKFNMPGNS+EPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR
Sbjct: 365 GVPYSLKFNMPGNSTEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 424 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHR 483
           DLESVDRKKTPF+VVQGHRPMYTTSNELRDAPLREKMLHHLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFIVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 484 YERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 543
           YERFCPLNNYTCGSMG+DGE WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 544 MYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSIANS 603
           MYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKF NSSIANS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFINSSIANS 604

Query: 604 TTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
           TT N MLEFSFSWYVMG SILVLGAF+GYIIGFVSHARKNS+SRNNWTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSLSRNNWTPVKTEELQ 660

BLAST of Lsi02G017370 vs. NCBI nr
Match: XP_008465701.1 (PREDICTED: probable inactive purple acid phosphatase 2 [Cucumis melo])

HSP 1 Score: 1295.8 bits (3352), Expect = 0.0e+00
Identity = 616/656 (93.90%), Postives = 633/656 (96.49%), Query Frame = 0

Query: 4   LIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSS 63
           +IP ILL SF+P+S QSKVSIS SPSILSKSGD V IQWSGIESPS LDWLGIYSPPNSS
Sbjct: 5   IIPFILLFSFVPLSFQSKVSISLSPSILSKSGDSVHIQWSGIESPSNLDWLGIYSPPNSS 64

Query: 64  HKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 123
           HKHF+GY+FLSSSPTW+SG GSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG
Sbjct: 65  HKHFVGYLFLSSSPTWESGYGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 124 TAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 183
           TAHLLAAS+ELRF+ G GPEQIHLAFTD DDEMRVMFVTEDGSERYVRYGEKKEKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 184

Query: 184 VARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNF 243
           VA VERYEREHMCDSPANDSIGWRDPGFIHDAVMNKL+KGAKVYYQVGSDSKGWSSILNF
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 244 VSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISHIGD 303
           VSRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPA +SHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 304 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGEC 363
           ISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWP+QPWKPEWA+GIYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYGIYGKDGGGEC 364

Query: 364 GVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 423
           GVPYSLKFNMPGN SEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR
Sbjct: 365 GVPYSLKFNMPGNFSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 424 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHR 483
           DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 484 YERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 543
           YERFCPLNNYTCGSMG+DGE WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 544 MYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSIANS 603
           MYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQVLNG VGAKF +SSIANS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGNVGAKFIDSSIANS 604

Query: 604 TTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
           TT N MLEFSFSWYVMG SILVLGAF+GYIIGFVSHARKNSISRNNWTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSISRNNWTPVKTEELQ 660

BLAST of Lsi02G017370 vs. NCBI nr
Match: XP_022973269.1 (probable inactive purple acid phosphatase 2 [Cucurbita maxima])

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 589/659 (89.38%), Postives = 619/659 (93.93%), Query Frame = 0

Query: 1   MNNLIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPP 60
           M NLI LILLIS  PISIQSKVSIS SPS L+KSGDPVQIQWSG+ESPS LDWLGIYSPP
Sbjct: 30  MENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESPSNLDWLGIYSPP 89

Query: 61  NSSHKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNP 120
           NSSHKHFIGYVFLSSSP W+SGSGSVSIPLVNLRS YSFRIFRWTESEIDDKHHDHD+NP
Sbjct: 90  NSSHKHFIGYVFLSSSPGWESGSGSVSIPLVNLRSKYSFRIFRWTESEIDDKHHDHDNNP 149

Query: 121 LPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLD 180
           LPGT HLLAASEELRFS G GPEQIHLAFTD DDEMRVMFVTEDGSER+VRYGEK+E+LD
Sbjct: 150 LPGTKHLLAASEELRFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSERFVRYGEKEEELD 209

Query: 181 QIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSI 240
           QIV ARV RYEREHMCDSPANDSIGWRDPGFIHDAVM KLQ G++VYYQVGS+SKGWSSI
Sbjct: 210 QIVAARVARYEREHMCDSPANDSIGWRDPGFIHDAVMTKLQNGSRVYYQVGSESKGWSSI 269

Query: 241 LNFVSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISH 300
            +F+SRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPAF+SH
Sbjct: 270 QSFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAFVSH 329

Query: 301 IGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGG 360
           IGDISYARGHAWLWDVFFNQIEPVAS+VAYHVCIGNHEYDWP+QPWKP+WA GIYGKDGG
Sbjct: 330 IGDISYARGHAWLWDVFFNQIEPVASRVAYHVCIGNHEYDWPLQPWKPDWASGIYGKDGG 389

Query: 361 GECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF 420
           GECGVPYSLKFNMPGNSSEPT+++ +PTRNLFYSFNMGSVHFVYISTETNFL+GS Q+ F
Sbjct: 390 GECGVPYSLKFNMPGNSSEPTKTNGVPTRNLFYSFNMGSVHFVYISTETNFLKGSRQFSF 449

Query: 421 IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGH 480
           ++RDLESVDRKKTPFVVVQGHRPMYTTSNE RD PLREKMLHHLEPLFVKNNVTLALWGH
Sbjct: 450 LERDLESVDRKKTPFVVVQGHRPMYTTSNERRDTPLREKMLHHLEPLFVKNNVTLALWGH 509

Query: 481 VHRYERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQP 540
           VHRYERFCPL NYTCGSMGVDGE WEALPVHLVIGMAGQDWQPIWEPRPNH D PIFPQP
Sbjct: 510 VHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHLDVPIFPQP 569

Query: 541 KRSMYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSI 600
           KRSMYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQV+NGGVGAKF N S+
Sbjct: 570 KRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVNGGVGAKF-NESV 629

Query: 601 ANSTTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
            NS +RN MLE SFSWYVMG SILVLGAF+GYI+GFVSHARK   SRNNWTPVKTEELQ
Sbjct: 630 ENSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRNNWTPVKTEELQ 684

BLAST of Lsi02G017370 vs. NCBI nr
Match: XP_022951598.1 (probable inactive purple acid phosphatase 2 [Cucurbita moschata])

HSP 1 Score: 1232.6 bits (3188), Expect = 0.0e+00
Identity = 588/659 (89.23%), Postives = 619/659 (93.93%), Query Frame = 0

Query: 1   MNNLIPLILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPP 60
           M NLI LILLIS  PISIQSKVSIS SPS L+KSGDPVQIQWSG+ESPSKLDWLGIYSPP
Sbjct: 1   MENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESPSKLDWLGIYSPP 60

Query: 61  NSSHKHFIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNP 120
           NSSHKHFIGYVFLSSSP W+SGSGSV+IPLVNLRS YSFRIFRWTESEIDDKHHDHD+NP
Sbjct: 61  NSSHKHFIGYVFLSSSPGWESGSGSVTIPLVNLRSKYSFRIFRWTESEIDDKHHDHDNNP 120

Query: 121 LPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLD 180
           LPGT HLLA+SEELRFS G GPEQIHLAFTD DDEMRVMFVTEDGSER VRYGEK+E+LD
Sbjct: 121 LPGTKHLLASSEELRFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSERLVRYGEKEEELD 180

Query: 181 QIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSI 240
           QIV ARV RYER+HMCDSPANDSIGWRDPGFIHDAVM  LQ G++VYYQVGS+SKGWSSI
Sbjct: 181 QIVAARVARYERKHMCDSPANDSIGWRDPGFIHDAVMTNLQNGSRVYYQVGSESKGWSSI 240

Query: 241 LNFVSRNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISH 300
            NF+SRNEDSDETIAFLFGDMGAATPY TFVRTQ+ESISTVRWILRDIEALGDKPAF+SH
Sbjct: 241 QNFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAFVSH 300

Query: 301 IGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGG 360
           IGDISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWP+QPWKP+WA GIYGKDGG
Sbjct: 301 IGDISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPDWASGIYGKDGG 360

Query: 361 GECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF 420
           GECGVPYSLKFNMPGNSSEPT+++ +PTRNLFYSFNMGSVHFVYISTETNFL+GS Q+ F
Sbjct: 361 GECGVPYSLKFNMPGNSSEPTKANGVPTRNLFYSFNMGSVHFVYISTETNFLKGSRQFSF 420

Query: 421 IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGH 480
           ++RDLESVDRKKTPFVVVQGHRPMYTTSNELRD PLREKMLHHLEPLFVKNNVTLALWGH
Sbjct: 421 LERDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLHHLEPLFVKNNVTLALWGH 480

Query: 481 VHRYERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQP 540
           VHRYERFCPL NYTCGSMGVDGE WEALPVHLVIGMAGQDWQPIWEPRPNH D PIFPQP
Sbjct: 481 VHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHLDVPIFPQP 540

Query: 541 KRSMYRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSI 600
           KRSMYRGGEFGYTRL ATKEKLTISYVGNHDGEVHDSVEILASGQV+NGGVGAKF N S+
Sbjct: 541 KRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVNGGVGAKF-NESV 600

Query: 601 ANSTTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEELQ 660
            NS +RN MLE SFSWYVMG SILVLGAF+GYI+GFVSHARK   SRNNWTPVKTEELQ
Sbjct: 601 KNSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRNNWTPVKTEELQ 655

BLAST of Lsi02G017370 vs. TAIR 10
Match: AT1G13900.1 (Purple acid phosphatases superfamily protein )

HSP 1 Score: 946.0 bits (2444), Expect = 1.6e-275
Identity = 447/654 (68.35%), Postives = 526/654 (80.43%), Query Frame = 0

Query: 7   LILLISFLPISIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSSHKH 66
           L+L +S    S  SK +IS SP+ L++SGD V IQWSG++SPS LDWLG+YSPP S + H
Sbjct: 9   LLLFVSVFVSSADSKATISISPNALNRSGDSVVIQWSGVDSPSDLDWLGLYSPPESPNDH 68

Query: 67  FIGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPGTAH 126
           FIGY FL+ S TW+ G GS+S+PL NLRSNY+FRIFRW+ESEID KH DHD NPLPGT H
Sbjct: 69  FIGYKFLNESSTWKDGFGSISLPLTNLRSNYTFRIFRWSESEIDPKHKDHDQNPLPGTKH 128

Query: 127 LLAASEELRFSTGSG-PEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIVVA 186
           LLA SE+L F +G G PEQIHL+FT+  + MRVMFV  DG ER+VRYGE K+ L     A
Sbjct: 129 LLAESEQLTFGSGVGMPEQIHLSFTNMVNTMRVMFVAGDGEERFVRYGESKDLLGNSAAA 188

Query: 187 RVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNFVS 246
           R  RYEREHMCDSPAN +IGWRDPG+I D VM  L  G + YYQVGSDSKGWS I ++++
Sbjct: 189 RGMRYEREHMCDSPANSTIGWRDPGWIFDTVMKNLNDGVRYYYQVGSDSKGWSEIHSYIA 248

Query: 247 RNEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALGDKPAFISHIGDIS 306
           R+  ++ET+AF+FGDMG ATPY TF+RTQ+ESISTV+WILRDIEALGDKPA ISHIGDIS
Sbjct: 249 RDVTAEETVAFMFGDMGCATPYTTFIRTQDESISTVKWILRDIEALGDKPAMISHIGDIS 308

Query: 307 YARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGECGV 366
           YARG++W+WD FF Q+EP+AS V YHVCIGNHEYD+  QPWKP+WA  IYG DGGGECGV
Sbjct: 309 YARGYSWVWDEFFAQVEPIASTVPYHVCIGNHEYDFSTQPWKPDWAASIYGNDGGGECGV 368

Query: 367 PYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKRDL 426
           PYSLKFNMPGNSSE T   + PTRNL+YS++MG+VHFVYISTETNFL+G SQYEFIKRDL
Sbjct: 369 PYSLKFNMPGNSSESTGMKAPPTRNLYYSYDMGTVHFVYISTETNFLKGGSQYEFIKRDL 428

Query: 427 ESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHRYE 486
           ESVDRKKTPFVVVQGHRPMYTTSNE+RD  +R+KM+ HLEPLFVKNNVTLALWGHVHRYE
Sbjct: 429 ESVDRKKTPFVVVQGHRPMYTTSNEVRDTMIRQKMVEHLEPLFVKNNVTLALWGHVHRYE 488

Query: 487 RFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMY 546
           RFCP++N TCG+       W+  PVHLVIGMAGQDWQPIW+PRPNHPD PIFPQP++SMY
Sbjct: 489 RFCPISNNTCGTQ------WQGNPVHLVIGMAGQDWQPIWQPRPNHPDLPIFPQPEQSMY 548

Query: 547 RGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGG-VGAKFSN-SSIANS 606
           R GEFGYTRL A KEKLT+S+VGNHDGEVHD+VE+LASG V++G     K  N  ++  S
Sbjct: 549 RTGEFGYTRLVANKEKLTVSFVGNHDGEVHDTVEMLASGVVISGSKESTKIPNLKTVPAS 608

Query: 607 TTRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNSISRNNWTPVKTEE 658
            T     E +  WY  GA ++V+G  +G+IIGF +  +K+S S N W PVK EE
Sbjct: 609 ATLMGKSESNALWYAKGAGLMVVGVLLGFIIGFFTRGKKSS-SGNRWIPVKNEE 655

BLAST of Lsi02G017370 vs. TAIR 10
Match: AT2G03450.1 (purple acid phosphatase 9 )

HSP 1 Score: 909.4 bits (2349), Expect = 1.6e-264
Identity = 437/656 (66.62%), Postives = 517/656 (78.81%), Query Frame = 0

Query: 9   LLISFLPI-SIQSKVSISFSPSILSKSGDPVQIQWSGIESPSKLDWLGIYSPPNSSHKHF 68
           L   FL I S+ SK +IS SP  L++SGD V I+WSG+ESPS LDWLGIYSPP+S H HF
Sbjct: 8   LFFFFLLISSVYSKATISISPQTLNRSGDIVVIKWSGVESPSDLDWLGIYSPPDSPHDHF 67

Query: 69  IGYVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPGTAHL 128
           IGY FLS SPTWQSGSGS+S+PL NLRSNY+FRIF WT+SEI+ KH DHDHNPLPGT HL
Sbjct: 68  IGYKFLSDSPTWQSGSGSISLPLTNLRSNYTFRIFHWTQSEINPKHQDHDHNPLPGTRHL 127

Query: 129 LAASEELRFSTG-SGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEKKEKLDQIVVAR 188
           L  S +L F    + PEQIHL++TD+ +EMRV+FVT DG ER  RYGE K+KLD I VAR
Sbjct: 128 LTESNQLNFRFAVNRPEQIHLSYTDNINEMRVVFVTGDGEEREARYGEVKDKLDNIAVAR 187

Query: 189 VERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSDSKGWSSILNFVSR 248
             RYE EHMC +PAN ++GWRDPG+  DAVM  L++G + YYQVGSD KGWS I +FVSR
Sbjct: 188 GVRYEIEHMCHAPANSTVGWRDPGWTFDAVMKNLKQGIRYYYQVGSDLKGWSEIHSFVSR 247

Query: 249 NEDSDETIAFLFGDMGAATPYMTFVRTQEESISTVRWILRDIEALG-DKPAFISHIGDIS 308
           NE S+ET+AF+FGDMG  TPY TF+R +EES+STV+WILRDIEALG DKP  +SHIGDIS
Sbjct: 248 NEGSEETLAFMFGDMGCYTPYTTFIRGEEESLSTVKWILRDIEALGDDKPVIVSHIGDIS 307

Query: 309 YARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPEWAFGIYGKDGGGECGV 368
           YARG++W+WD FF QIEP+ASKV YHVCIGNHEYDWP QPWKP+WA  +YGKD GGECGV
Sbjct: 308 YARGYSWIWDEFFTQIEPIASKVPYHVCIGNHEYDWPNQPWKPDWAAYVYGKDSGGECGV 367

Query: 369 PYSLKFNMPGNSSEPTESHSLP-TRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKRD 428
           PYS+KFNMPGNS+E T     P +RNL+YS++MGSVHFVYISTET+FL+G  QY F+K D
Sbjct: 368 PYSVKFNMPGNSTEATGMVKGPQSRNLYYSYDMGSVHFVYISTETDFLKGGKQYSFLKSD 427

Query: 429 LESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLFVKNNVTLALWGHVHRY 488
           LESV+R KTPFVVVQGHRPMYTTS ++RDA +REKM+ HLEPL VKNNVT+ALWGHVHRY
Sbjct: 428 LESVNRSKTPFVVVQGHRPMYTTSRKIRDAAIREKMIEHLEPLLVKNNVTVALWGHVHRY 487

Query: 489 ERFCPLNNYTCGSMGVDGEGWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSM 548
           ERFC ++N TC      GE W+  PVHLVIGMAG+D QP+WEPR NH D PIFPQP  SM
Sbjct: 488 ERFCAISNNTC------GERWQGNPVHLVIGMAGKDSQPMWEPRANHEDVPIFPQPANSM 547

Query: 549 YRGGEFGYTRLFATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFSNSSIANST 608
           YRGGEFGY RL A KE+LT+SYVGNHDGEVHD VEILASG+V++G      S+    +S 
Sbjct: 548 YRGGEFGYIRLVANKERLTLSYVGNHDGEVHDVVEILASGEVISG------SDDGTKDSN 607

Query: 609 TRNEMLEFSFSWYVMGASILVLGAFVGYIIGFVSHARKNS---ISRNNWTPVKTEE 658
             +E  +F+  WY+ GAS++V+G   GY +GF+S  +K S    S  +W  VK EE
Sbjct: 608 FGSES-DFAVLWYIEGASVMVVGVIFGYFVGFLSRKKKESGVGSSNRSWIQVKNEE 650

BLAST of Lsi02G017370 vs. TAIR 10
Match: AT1G13750.1 (Purple acid phosphatases superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 1.3e-59
Identity = 180/609 (29.56%), Postives = 279/609 (45.81%), Query Frame = 0

Query: 14  LPISIQSKVSISFSPSILSKSG---DPVQIQWSGIESPSKLDWLGIYSPPNSSHKHFIG- 73
           +   +  K  +  SP++L  +G   + V +Q+S    PS  DW+G++SP + +     G 
Sbjct: 38  ITFGLNEKAYVKASPTVLGSNGQHSELVLVQYSS-PKPSDDDWIGVFSPADFNASTCPGD 97

Query: 74  -----------------YVFLSSSPTWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDK 133
                            Y   S+     +G+GS+ + L+N RS++SF +F  +   ++ K
Sbjct: 98  NKMVQPPRLCSAPVKFQYANFSNPRYTNTGTGSLKLQLINQRSDFSFALF--SGGLLNPK 157

Query: 134 HHDHDHNPLPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDG---SERY 193
                         L+A S ++ F   + P    LA     DEM V + +  G   +E  
Sbjct: 158 --------------LVAISNKVAFENPNAPVYPRLALGKEWDEMTVTWTSGYGLNLAEPV 217

Query: 194 VRYGEKKEKLDQIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQ 253
           V +G K  +  ++  A    + R  MC +PA  ++GWRDPG+IH A + +L   +K  Y+
Sbjct: 218 VEWGVKGGE-RKLSPAGTLTFARNSMCGAPAR-TVGWRDPGYIHTAFLKELWPNSKYTYR 277

Query: 254 VGSD-SKG---WSSILNFVSRNEDSDETI--AFLFGDMGAA-----TPYMTFVRTQEESI 313
           VG   S G   WS    F S       ++    +FGDMG A     + Y  F   Q  S+
Sbjct: 278 VGHRLSNGALIWSKEYQFKSSPFPGQNSVQQVVIFGDMGKAEVDGSSEYNDF---QRASL 337

Query: 314 STVRWILRDIEALGDKPAFISHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHE 373
           +T + +++D++    K   + HIGDI YA G+   WD F  QIEP+AS V Y +  GNHE
Sbjct: 338 NTTKQLIKDLK----KTDAVFHIGDICYANGYLSQWDQFIAQIEPIASTVPYMIASGNHE 397

Query: 374 YDWPVQPWKPEWAFGIYGKDGGGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMG 433
             WP      E      G D GGECGVP    F +P  +             ++YS + G
Sbjct: 398 RVWPNSGSFYE------GLDSGGECGVPAETMFYVPAQN----------RAKVWYSSDYG 457

Query: 434 SVHFVYISTETNFLQGSSQYEFIKRDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDAPL 493
              F    TE ++ +G+ QY FI+  L SVDR+K P+++   HR +  +S      +   
Sbjct: 458 MFRFCVADTEHDWREGTEQYNFIEHCLASVDRQKQPWLIFLAHRVLGYSSTYFYAEEGSF 517

Query: 494 REKM-LHHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMGVDGEGWEAL---PVHL 553
            E M    L+ L+ K  V +A++GH H YER CP+    C S   +   ++A     +H+
Sbjct: 518 AEPMGRESLQKLWQKYKVDIAIYGHAHNYERTCPVYQSVCTSH--EKSNYKAPLNGTIHI 577

Query: 554 VIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLFA-TKEKLTISYVGNHD 581
           V G  G       + +PN            S++R  ++G+ +L A     L   Y  + D
Sbjct: 578 VAGGGGAGLAEFSDLQPNW-----------SLFRDYDYGFLKLTAIDHSNLLFEYKKSSD 591

BLAST of Lsi02G017370 vs. TAIR 10
Match: AT5G50400.1 (purple acid phosphatase 27 )

HSP 1 Score: 227.3 bits (578), Expect = 3.7e-59
Identity = 177/600 (29.50%), Postives = 271/600 (45.17%), Query Frame = 0

Query: 24  ISFSPSILSKSGDPVQIQWSGI----ESPSKLDWLGIYSPPN--------SSHKHF---- 83
           I  SP +L   G     +W  +      PS  DW+G++SP          +  K      
Sbjct: 47  IHVSPLVLGSQGQ--DTEWVNVVISNPEPSSDDWVGVFSPAKFDSSSCAPTDDKEIAPFI 106

Query: 84  ----IGYVFLSSSPTW-QSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLP 143
               + Y++  SSP + ++G+  +   L+N R+++SF +F    S           NP  
Sbjct: 107 CSAPVKYMYAKSSPDYMKTGNAVLKFMLINQRADFSFALFTGGLS-----------NPT- 166

Query: 144 GTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE--KKEKLD 203
               L++ S  + F     P    LA     DEM V + +       V + E  +K    
Sbjct: 167 ----LVSVSNHVSFINPKAPVYPRLALGKKWDEMTVTWTSGYNIGEAVPFVEWSRKGTRS 226

Query: 204 QIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVGSD----SKG 263
           +   A    + R  MC +PA  ++GWRDPGFIH A +  L    K  Y++G +    S  
Sbjct: 227 RRSPAGTLTFTRNSMCGAPAR-TVGWRDPGFIHTASLKDLWPNLKYTYRMGHELMNGSIV 286

Query: 264 WSSILNFVSRNEDSDETI--AFLFGDMG-----AATPYMTFVRTQEESISTVRWILRDIE 323
           WS    F S      +++    +FGDMG      +  Y  +   Q  S++T   +++D++
Sbjct: 287 WSKNFTFKSSPYPGQDSLQRVIIFGDMGKGERDGSNEYNDY---QPGSLNTTDQLIKDLK 346

Query: 324 ALGDKPAFISHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPVQPWKPE 383
            +      + HIGDI+YA G+   WD F  Q+EP+AS V Y V  GNHE DW      P+
Sbjct: 347 NID----IVFHIGDITYANGYISQWDQFTAQVEPIASTVPYMVASGNHERDW------PD 406

Query: 384 WAFGIYGKDGGGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTET 443
                 GKD GGECGVP    F+ P  +              +YS + G   F    TE 
Sbjct: 407 SGSFYGGKDSGGECGVPAETMFDFPAENK----------AKFWYSADYGMFRFCVADTEH 466

Query: 444 NFLQGSSQYEFIKRDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDAPLREKM-LHHLEP 503
           ++ +GS QY+FI+R L SVDR+  P+++   HR +  ++N+   ++    E M    L+ 
Sbjct: 467 DWREGSEQYQFIERCLASVDRRAQPWLIFIAHRVLGYSTNDWYGQEGSFEEPMGRESLQK 526

Query: 504 LFVKNNVTLALWGHVHRYERFCPLNNYTCGSMGVDGE-----GWEALPVHLVIGMAGQDW 563
           L+ K  V +A +GHVH YER CP+    C    +D E     G     +H+V+G AG   
Sbjct: 527 LWQKYKVDIAFYGHVHNYERTCPIYQNQC----MDNEKSHYSGAFKGTIHVVVGGAG--- 586

Query: 564 QPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLFA-TKEKLTISYVGNHDGEVHDSVEI 581
                   +H       +PK S++R  ++G+ +L A     L   Y  + +G VHDS  I
Sbjct: 587 --------SHLSSFSSLKPKWSIFRDYDYGFVKLTAFDHSSLLFEYKKSSNGAVHDSFTI 589

BLAST of Lsi02G017370 vs. TAIR 10
Match: AT4G24890.1 (purple acid phosphatase 24 )

HSP 1 Score: 224.6 bits (571), Expect = 2.4e-58
Identity = 171/606 (28.22%), Postives = 272/606 (44.88%), Query Frame = 0

Query: 16  ISIQSKVSISFSPSILSKSGDPVQIQWSGI----ESPSKLDWLGIYSP---------PNS 75
           +++ S V +  SP +L   G+    +W  +      P+  DW+G++SP         P S
Sbjct: 42  LALDSSVKLHASPQVLGSQGE--DTEWVNLAISNPKPTSDDWIGVFSPAKFDSGNCWPTS 101

Query: 76  SHKHF--------IGYVFLSSSPTW-QSGSGSVSIPLVNLRSNYSFRIFRWTESEIDDKH 135
             K          I Y++ +S P + +SG+ ++   ++N R++ SF +F     E     
Sbjct: 102 GGKEKTPYICSSPIKYMYCNSHPDYMKSGNVTLKFQIINQRADVSFALFSNGVQE----- 161

Query: 136 HDHDHNPLPGTAHLLAASEELRFSTGSGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYG 195
                       HLL  S  + F     P    LA   + DEM V + +    +  V + 
Sbjct: 162 -----------PHLLGVSNPVAFFNPKAPVYPRLALGKNWDEMTVTWTSGYNIDEAVPFI 221

Query: 196 EKKEK--LDQIVVARVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLQKGAKVYYQVG 255
           E   K    +   A    + R  MC +PA   +GWRDPGF H + + +L    +  Y++G
Sbjct: 222 EWSAKGLPARRSPAGTLTFNRNSMCGNPAR-GVGWRDPGFFHTSFLKELWPNREYIYRLG 281

Query: 256 SD----SKGWSSILNFVSR---NEDSDETIAFLFGDMG-----AATPYMTFVRTQEESIS 315
            D    S  WS    FVS     +DS + +  +FGDMG      +  Y  +   Q  S++
Sbjct: 282 HDLVNGSTIWSKNYTFVSSPYPGQDSKQRV-IIFGDMGKGERDGSNEYNDY---QPGSLN 341

Query: 316 TVRWILRDIEALGDKPAFISHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEY 375
           T   +++D++ +      + HIGD++Y+ G+   WD F  Q++P+AS V Y +  GNHE 
Sbjct: 342 TTDQVIKDLKDID----IVFHIGDLTYSNGYLSQWDQFTAQVQPIASTVPYMIASGNHER 401

Query: 376 DWPVQPWKPEWAFGIYGKDGGGECGVPYSLKFNMPGNSSEPTESHSLPTRNLFYSFNMGS 435
           DW      P+      G D GGECGVP    F  P  +              +Y  + G 
Sbjct: 402 DW------PDTGSFYAGTDSGGECGVPAETMFYFPAEN----------RAKFWYKTDYGM 461

Query: 436 VHFVYISTETNFLQGSSQYEFIKRDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDAPLR 495
             F    +E ++ +G+ QY+FI+  L +VDRK  P+++   HR +  ++N+   ++    
Sbjct: 462 FRFCVADSEHDWREGTEQYKFIENCLATVDRKTQPWLIFIAHRVLGYSTNDWYGKEGTFE 521

Query: 496 EKM-LHHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMGVDG-EGWEALPVHLVIG 555
           E M    L+ L+ K  V LA +GHVH YER CP+    C +   D   G     +H+V+G
Sbjct: 522 EPMGRESLQKLWQKYKVDLAFYGHVHNYERTCPIYESQCVNNDKDHYSGTFKGTIHVVVG 581

Query: 556 MAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLFAT-KEKLTISYVGNHDGEV 581
            AG    P                PK S+ R  +FG+ +L A+    L   Y  +  G+V
Sbjct: 582 GAGSHLSPFSS-----------LVPKWSLVRDYDFGFVKLTASDHSSLLFEYKKSSTGQV 593

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMG72.2e-27468.35Probable inactive purple acid phosphatase 2 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9ZQ812.3e-26366.62Probable inactive purple acid phosphatase 9 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9LMX41.8e-5829.56Probable inactive purple acid phosphatase 1 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q5MAU85.2e-5829.50Probable inactive purple acid phosphatase 27 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8H1R23.4e-5728.22Probable inactive purple acid phosphatase 24 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KNH80.0e+0093.60Purple acid phosphatase OS=Cucumis sativus OX=3659 GN=Csa_5G487720 PE=3 SV=1[more]
A0A1S3CPV00.0e+0093.90Purple acid phosphatase OS=Cucumis melo OX=3656 GN=LOC103503324 PE=3 SV=1[more]
A0A6J1IE320.0e+0089.38Purple acid phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111471840 PE=3 SV=1[more]
A0A6J1GI520.0e+0089.23Purple acid phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111454362 PE=3 SV=1[more]
A0A6J1D9S20.0e+0083.64Purple acid phosphatase OS=Momordica charantia OX=3673 GN=LOC111018532 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038890179.10.0e+0095.45probable inactive purple acid phosphatase 2 [Benincasa hispida][more]
XP_004143791.10.0e+0093.60probable inactive purple acid phosphatase 2 [Cucumis sativus] >KGN51195.1 hypoth... [more]
XP_008465701.10.0e+0093.90PREDICTED: probable inactive purple acid phosphatase 2 [Cucumis melo][more]
XP_022973269.10.0e+0089.38probable inactive purple acid phosphatase 2 [Cucurbita maxima][more]
XP_022951598.10.0e+0089.23probable inactive purple acid phosphatase 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G13900.11.6e-27568.35Purple acid phosphatases superfamily protein [more]
AT2G03450.11.6e-26466.62purple acid phosphatase 9 [more]
AT1G13750.11.3e-5929.56Purple acid phosphatases superfamily protein [more]
AT5G50400.13.7e-5929.50purple acid phosphatase 27 [more]
AT4G24890.12.4e-5828.22purple acid phosphatase 24 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004843Calcineurin-like phosphoesterase domain, ApaH typePFAMPF00149Metallophoscoord: 258..484
e-value: 2.0E-16
score: 61.1
NoneNo IPR availableGENE3D2.60.40.380coord: 141..248
e-value: 1.0E-16
score: 63.0
NoneNo IPR availablePANTHERPTHR45778:SF11INACTIVE PURPLE ACID PHOSPHATASE 9-RELATEDcoord: 14..606
NoneNo IPR availablePANTHERPTHR45778PURPLE ACID PHOSPHATASE-RELATEDcoord: 14..606
IPR029052Metallo-dependent phosphatase-likeGENE3D3.60.21.10coord: 250..587
e-value: 1.5E-78
score: 266.5
IPR029052Metallo-dependent phosphatase-likeSUPERFAMILY56300Metallo-dependent phosphatasescoord: 257..576
IPR025733Iron/zinc purple acid phosphatase-like C-terminal domainPFAMPF14008Metallophos_Ccoord: 509..577
e-value: 5.1E-15
score: 56.1
IPR015914Purple acid phosphatase, N-terminalPFAMPF16656Pur_ac_phosph_Ncoord: 142..243
e-value: 2.6E-16
score: 59.9
IPR041792Purple acid phosphatase, metallophosphatase domainCDDcd00839MPP_PAPscoord: 249..580
e-value: 5.60553E-102
score: 311.155
IPR008963Purple acid phosphatase-like, N-terminalSUPERFAMILY49363Purple acid phosphatase, N-terminal domaincoord: 128..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G017370.1Lsi02G017370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003993 acid phosphatase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016787 hydrolase activity