Homology
BLAST of Lsi02G017140 vs. ExPASy Swiss-Prot
Match:
F4HW04 (DNA polymerase epsilon catalytic subunit A OS=Arabidopsis thaliana OX=3702 GN=POL2A PE=1 SV=1)
HSP 1 Score: 1895.9 bits (4910), Expect = 0.0e+00
Identity = 985/1505 (65.45%), Postives = 1114/1505 (74.02%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S NRRRD +D + KK ++ +AE+++ESK GF LFSEG+ RLGWLLTF+SSS +D D
Sbjct: 2 SGDNRRRDRKDTRWS-KKPKVVNTAEDELESKLGFGLFSEGETRLGWLLTFSSSSWEDRD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KVYSC+DLYFVTQDG SFK+KYKFRPYFYAATK+KME ++EAYLRRRY+ Q+ADIEI+
Sbjct: 62 TGKVYSCVDLYFVTQDGFSFKTKYKFRPYFYAATKDKMELELEAYLRRRYERQVADIEIV 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL K YLK+SFDTVQ+LM VK +LLH+VE N+ K DA EAY+SIL GK
Sbjct: 122 EKEDLDLKNHLSGLQKKYLKISFDTVQQLMEVKRDLLHIVERNQAKFDALEAYESILAGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQR QD L+ IVDLREYDVPYHVRFAID+D+R GQWY+VS+SST + LEKR DLLQRAE
Sbjct: 182 REQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
VR+CAFDIETTKLPLKFPDAEYD IMMISYMVDGQG+LIINRECVGED+E+LEYTPKPE+
Sbjct: 242 VRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQGFLIINRECVGEDVEDLEYTPKPEF 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+G+FKV NVKNE+ELL+ WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+G
Sbjct: 302 EGYFKVTNVKNEVELLQRWFYHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
F+CD+NQGECR+KFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 362 FRCDQNQGECRAKFACHLDCFAWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFA EKPQ MASYSVSDAVATYYLYMTYV+PFIFSLATIIPM PDEVLRKG
Sbjct: 482 LEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVNPFIFSLATIIPMVPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMV+AY+ANVVCPNK+Q+DPEKF+ N+LLESETYIGGHVECLESGVFRSD+P
Sbjct: 542 SGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQNQLLESETYIGGHVECLESGVFRSDIP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSF+LDSSAY QLI+NL RDL+YAI VEGKM ++ +SNY +VK I EK+ ++ D
Sbjct: 602 TSFKLDSSAYQQLIDNLGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
+P+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 DPIREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P +++TDE+CTACDFNRPGKTCLR+LEWVW G F K+ DYYH
Sbjct: 782 --PPSIVTDEICTACDFNRPGKTCLRKLEWVWRGVTFMGKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSG-TVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKA 907
LK+QIESEFVD+G + SK FL+LPK++QQ+KLKERLKKYCQKAYKRVLDKP+TEVR+A
Sbjct: 842 LKKQIESEFVDAGANIMSSKSFLDLPKVDQQSKLKERLKKYCQKAYKRVLDKPITEVREA 901
Query: 908 GICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSL 967
GICMREN FY+DTVR LNKVWKGKLSEAKASGNSIKIQEAQDMVV+YDSL
Sbjct: 902 GICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNSIKIQEAQDMVVVYDSL 961
Query: 968 QLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGI 1027
QLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGI
Sbjct: 962 QLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGI 1021
Query: 1028 WCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSE 1087
WC LPGSFPENFTFKT + KKLTISYPCVMLNVDVA+NNTNDQYQTLVDPVRK Y+ SE
Sbjct: 1022 WCCLPGSFPENFTFKTIDMKKLTISYPCVMLNVDVAKNNTNDQYQTLVDPVRKTYKSHSE 1081
Query: 1088 CSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ 1147
CSIEFEVDGPYKAMI+PASKEEGILIKKRYAVFN DGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 CSIEFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTLAELKGFEIKRRGELKLIKVFQ 1141
Query: 1148 IYLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSS 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 KAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLAD 1267
AELFDKFLHGSTLEECYS VA+VA+RWLDLLD+QGKDIADSELLDYISESSTMSKSLAD
Sbjct: 1202 -AELFDKFLHGSTLEECYSAVAAVADRWLDLLDNQGKDIADSELLDYISESSTMSKSLAD 1241
Query: 1268 YGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEI 1327
YGEQKSCAVTTAKRLA+FLG TMV+DKGLRCQYIVACEPKGTPVSERAVPVAIF T+PE+
Sbjct: 1262 YGEQKSCAVTTAKRLAEFLGVTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFTTNPEV 1241
Query: 1328 MKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWL 1387
MKF++RKWCK SS+VGIR IIDWSYYKQRLSSAIQK+ITIPAAMQKV+NPVPRV+HPDWL
Sbjct: 1322 MKFHLRKWCKTSSDVGIRLIIDWSYYKQRLSSAIQKVITIPAAMQKVANPVPRVLHPDWL 1241
Query: 1388 HKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSS- 1447
HKKVREKDDKFRQ+K+V+MF+S+ N D + + N +DIEDF K+ S
Sbjct: 1382 HKKVREKDDKFRQRKLVDMFSSA------NKDVVLDTDLPVTKDNVEDIEDFCKENRPSV 1241
Query: 1448 SGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGW 1500
GPKP+ RSY+V + Q + + ES + F++ID++ +Y GW
Sbjct: 1442 KGPKPIARSYEV--------------NKKQSECEQQESWDTEFHDISFQNIDKSVNYQGW 1241
BLAST of Lsi02G017140 vs. ExPASy Swiss-Prot
Match:
F4IFN6 (DNA polymerase epsilon catalytic subunit B OS=Arabidopsis thaliana OX=3702 GN=POL2B PE=2 SV=1)
HSP 1 Score: 1786.5 bits (4626), Expect = 0.0e+00
Identity = 937/1482 (63.23%), Postives = 1069/1482 (72.13%), Query Frame = 0
Query: 31 SAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSK 90
SA +++E+K GF LFS+G+ RLGWLLTF SSS +D DT K +SC+DL+FVTQDGSSFK+K
Sbjct: 16 SAADELETKLGFGLFSQGETRLGWLLTFASSSWEDADTGKTFSCVDLFFVTQDGSSFKTK 75
Query: 91 YKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSF 150
YKFRPY YAATK+ ME +VEAYLRRRY+ Q+ADI+I+ KEDL LKNHLSGL K YLK+SF
Sbjct: 76 YKFRPYLYAATKDNMELEVEAYLRRRYERQVADIQIVHKEDLYLKNHLSGLQKKYLKVSF 135
Query: 151 DTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRLQDFLECIVDLREYDVPYH 210
DTVQ+L+ VK +LLH+VE N K +A EAY+SIL GKREQR QD L+ +VDLREYDVPYH
Sbjct: 136 DTVQQLVEVKRDLLHIVERNLAKFNALEAYESILSGKREQRPQDCLDSVVDLREYDVPYH 195
Query: 211 VRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICAFDIETTKLPLKFPDAEYD 270
VRFAID+D+R GQWY+VS+SST + LEKR DLLQRAEVR+CAFDIET KLPLKFPDAEYD
Sbjct: 196 VRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETVKLPLKFPDAEYD 255
Query: 271 MIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNELELLKLWFAHM 330
IMMISYMVDGQG+LI NRECVG+DIE+LEYTPKPE++G+FKV NV NE+ELL+ WF+HM
Sbjct: 256 QIMMISYMVDGQGFLITNRECVGKDIEDLEYTPKPEFEGYFKVTNVTNEVELLRKWFSHM 315
Query: 331 REVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRSKFACHLDCFAW 390
+E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+GF+CD+NQGECR+KF CHLDCF+W
Sbjct: 316 QELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFVCHLDCFSW 375
Query: 391 VKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMKSEIVNNEVMQYLFNIVIAPLSAP 450
VKRDSYLPQGS GLK
Sbjct: 376 VKRDSYLPQGSQGLK--------------------------------------------- 435
Query: 451 VSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYS 510
AVTK KLGYDPLEVNPEDMVRFA EKPQ MASYS
Sbjct: 436 --------------------------AVTKVKLGYDPLEVNPEDMVRFAMEKPQTMASYS 495
Query: 511 VSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNK 570
VSDAVATYYLYMTYVHPF+FSLATIIPM PDEVLRKGSGTLCEMLLMV+AY+ANVVCPNK
Sbjct: 496 VSDAVATYYLYMTYVHPFVFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNK 555
Query: 571 HQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINNLDRDLQY 630
+Q+DPEKF+ +LLESETYIGGHVECL+SGVFRSD+PTSF+LD+SAY QLI+NL RDL+Y
Sbjct: 556 NQADPEKFYQGKLLESETYIGGHVECLQSGVFRSDIPTSFKLDASAYQQLIDNLGRDLEY 615
Query: 631 AIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGDEPMREECPLIYHLDVAAMYPNII 690
AI VEGKM ++ VSN+ +VK I EK+ ++ D+P+REE PLIYHLDVAAMYPNII
Sbjct: 616 AITVEGKMRMDSVSNFDEVKEVIREKLEKLR-----DDPIREEGPLIYHLDVAAMYPNII 675
Query: 691 LTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRSFAGRFHFLTTLRHVTTYKSIEVT 750
LTNRLQ
Sbjct: 676 LTNRLQ------------------------------------------------------ 735
Query: 751 LGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSCLIPAAVITDEVCTACDFNRPGKT 810
P +++TDEVCTACDFN P KT
Sbjct: 736 ---------------------------------------PPSIVTDEVCTACDFNGPEKT 795
Query: 811 CLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYHLKRQIESEFVDSG-TVRLSKQFL 870
CLR+LEWVW G F+ + +YYHLK+QIESE VD+G ++ SK FL
Sbjct: 796 CLRKLEWVWRGVTFKGNKS--------------EYYHLKKQIESESVDAGANMQSSKPFL 855
Query: 871 ELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYIDTVR-------- 930
+LPK+EQQ+KLKERLKKYCQKAY RVLDKP+TEVR+AGICMREN FY+DTVR
Sbjct: 856 DLPKVEQQSKLKERLKKYCQKAYSRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYE 915
Query: 931 ---LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWY 990
LNKVWKGKLSEAKASGN IKIQEA DMVV+YDSLQLAHKCILNSFYGYVMRKGARWY
Sbjct: 916 YKTLNKVWKGKLSEAKASGNLIKIQEAHDMVVVYDSLQLAHKCILNSFYGYVMRKGARWY 975
Query: 991 SMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPENFTFKTKESKKLT 1050
SMEMAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGIWCALPGSFPENFTFKT + KK T
Sbjct: 976 SMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCALPGSFPENFTFKTIDMKKFT 1035
Query: 1051 ISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPYKAMILPASKEEG 1110
ISYPCV+LNVDVA+NN+NDQYQTLVDPVRK Y RSECSIEFEVDGPYKAMI+PASKEEG
Sbjct: 1036 ISYPCVILNVDVAKNNSNDQYQTLVDPVRKTYNSRSECSIEFEVDGPYKAMIIPASKEEG 1095
Query: 1111 ILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQIYLYPYSSGMAKSYNIDAHADMH 1170
ILIKKRYAVFN DGT+AELKGFE+KRRGELKLIKVFQ
Sbjct: 1096 ILIKKRYAVFNHDGTIAELKGFEMKRRGELKLIKVFQ----------------------- 1155
Query: 1171 PKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSKAELFDKFLHGSTLEECYSVVAS 1230
AELFDKFLHGSTLEECYS VA+
Sbjct: 1156 --------------------------------------AELFDKFLHGSTLEECYSAVAA 1215
Query: 1231 VANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAKRLADFLGDTM 1290
VANRWLDLL+ QGKDIADSELLDYISESSTMSKSLADYG+QKSCAVTTAKRLADFLGDTM
Sbjct: 1216 VANRWLDLLEGQGKDIADSELLDYISESSTMSKSLADYGQQKSCAVTTAKRLADFLGDTM 1225
Query: 1291 VRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKISSEVGIRSIIDW 1350
V+DKGLRCQYIVA EP+GTPVSERAVPVAIF+TD KFY++KWCKISS GIRSIIDW
Sbjct: 1276 VKDKGLRCQYIVAREPEGTPVSERAVPVAIFQTDDPEKKFYLQKWCKISSYTGIRSIIDW 1225
Query: 1351 SYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDKFRQQKIVNMFASS 1410
YYKQRL SAIQK+ITIPAAMQKV+NPV RV HP WL KKV DKFRQ KIV+MF+S+
Sbjct: 1336 MYYKQRLHSAIQKVITIPAAMQKVANPVLRVRHPYWLEKKV---CDKFRQGKIVDMFSSA 1225
Query: 1411 RGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSS-SGPKPVVRSYDVRAMKHSVTRDS 1470
N D +++ NV+ DIE+F K+ S GPKPV RS++V HS
Sbjct: 1396 ------NKDHSTTQDNVV-----ADIEEFCKENRPSVKGPKPVARSFEV-DRNHS----- 1225
Query: 1471 QVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1500
+ K ES + +++D+N DY GWLE++K
Sbjct: 1456 --------EGKQQESWDPEFHDISLQNVDKNVDYQGWLELEK 1225
BLAST of Lsi02G017140 vs. ExPASy Swiss-Prot
Match:
Q07864 (DNA polymerase epsilon catalytic subunit A OS=Homo sapiens OX=9606 GN=POLE PE=1 SV=5)
HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 651/1425 (45.68%), Postives = 850/1425 (59.65%), Query Frame = 0
Query: 34 EDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKF 93
+ ++ +FGF E ++ GWL+ + DED +++ S +D YF+ DGS FK +
Sbjct: 42 DKMDLRFGFERLKEPGEKTGWLINMHPTEILDED-KRLGSAVDYYFIQDDGSRFKVALPY 101
Query: 94 RPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSFDTV 153
+PYFY AT+ E +V ++L ++++G+IA +E + KEDLDL NHL GL + Y++LSF TV
Sbjct: 102 KPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTV 161
Query: 154 QELMNVKSELLHVVESNREKSDAAEAYDSILFG------------KREQRLQDFLECIVD 213
++L+ V+ E+ V+ NRE+ A++AY ++L + +++ D L+ IVD
Sbjct: 162 EDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVD 221
Query: 214 LREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLE--KRKDLLQRAEVRICAFDIETTK 273
+REYDVPYH+R +ID + WY+V +E +R DL++R + + AFDIETTK
Sbjct: 222 MREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTK 281
Query: 274 LPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNE 333
LPLKFPDAE D IMMISYM+DGQGYLI NRE V EDIE+ E+TPKPEY+G F V N +E
Sbjct: 282 LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDE 341
Query: 334 LELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRS 393
L++ WF H++E KP I VTYNGD+FDWPF+E+RAA HGL M E+GFQ D +QGE ++
Sbjct: 342 AHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKD-SQGEYKA 401
Query: 394 KFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMKSEIVNNEVMQYL 453
H+DC WVKRDSYLP GSH
Sbjct: 402 PQCIHMDCLRWVKRDSYLPVGSH------------------------------------- 461
Query: 454 FNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDPLEVNPEDMVRFA 513
NL A KAKLGYDP+E++PEDM R A
Sbjct: 462 ----------------------------------NLKAAAKAKLGYDPVELDPEDMCRMA 521
Query: 514 KEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQ 573
E+PQ +A+YSVSDAVATYYLYM YVHPFIF+L TIIPM PDEVLRKGSGTLCE LLMVQ
Sbjct: 522 TEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQ 581
Query: 574 AYEANVVCPNKHQSDPEKF-HNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYD 633
A+ AN++ PNK + + K + +L+SETY+GGHVE LESGVFRSD+P FR++ +A+D
Sbjct: 582 AFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFD 641
Query: 634 QLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGDEPMREECPLIY 693
L+ +++ L++A++ E K+ +E V+N+ +V I K+A + D P R ECPLIY
Sbjct: 642 FLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLK-----DVPSRIECPLIY 701
Query: 694 HLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRSFAGRFHFLTTL 753
HLDV AMYPNIILTNRLQ
Sbjct: 702 HLDVGAMYPNIILTNRLQ------------------------------------------ 761
Query: 754 RHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSCLIPAAVITDEV 813
P+A++ +
Sbjct: 762 ---------------------------------------------------PSAMVDEAT 821
Query: 814 CTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYHLKRQIESE-FV 873
C ACDFN+PG C R++ W W GE A R +Y+ ++ Q+ESE F
Sbjct: 822 CAACDFNKPGANCQRKMAWQWRGEFMPASRS--------------EYHRIQHQLESEKFP 881
Query: 874 DSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYI 933
++ F EL + EQ K RL YC+KAYK++ V E R IC RENSFY+
Sbjct: 882 PLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEE-RLTTICQRENSFYV 941
Query: 934 DTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSF 993
DTVR L+KVWK KLS A G++ +++ ++M VLYDSLQLAHKCILNSF
Sbjct: 942 DTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSF 1001
Query: 994 YGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPEN 1053
YGYVMRKGARWYSMEMAG+V +TGA II AR LIE+IG+PLELDTDGIWC LP SFPEN
Sbjct: 1002 YGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPEN 1061
Query: 1054 FTFKTKESK--KLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDG 1113
F FKT K K+TISYP MLN+ V TNDQYQ L +P Y RSE SI FEVDG
Sbjct: 1062 FVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDG 1121
Query: 1114 PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQIYLYPYSSG 1173
PY AMILPASKEEG +KKRYAVFN+DG+LAELKGFE+KRRGEL+LIK+FQ
Sbjct: 1122 PYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQ--------- 1181
Query: 1174 MAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSKAELFDKFL 1233
+ +F+ FL
Sbjct: 1182 ----------------------------------------------------SSVFEAFL 1215
Query: 1234 HGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAV 1293
GSTLEE Y VA VA+ WLD+L S+ ++ DSEL + ISE+ +MS+ L DYGEQKS ++
Sbjct: 1242 KGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSI 1215
Query: 1294 TTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWC 1353
+TAKRLA+FLGD MV+D GL C+YI++ +P+G+PV+ERA+P+AIF+ +P + K ++RKW
Sbjct: 1302 STAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWL 1215
Query: 1354 KISS--EVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREK 1413
K SS + IR+I+DW YY +RL SAIQKIITIPAA+Q+V NPVPRV HPDWLHKK+ EK
Sbjct: 1362 KSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEK 1215
Query: 1414 DDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDF 1428
+D ++Q+KI +F + + + + S ++ D+EDF
Sbjct: 1422 NDVYKQKKISELFTLEGRRQVTMAEASEDSPR----PSAPDMEDF 1215
BLAST of Lsi02G017140 vs. ExPASy Swiss-Prot
Match:
Q9WVF7 (DNA polymerase epsilon catalytic subunit A OS=Mus musculus OX=10090 GN=Pole PE=1 SV=3)
HSP 1 Score: 1176.8 bits (3043), Expect = 0.0e+00
Identity = 642/1393 (46.09%), Postives = 835/1393 (59.94%), Query Frame = 0
Query: 34 EDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKF 93
+ ++ +FGF E +R GWL+ + DED +++ S +D YF+ DGS FK +
Sbjct: 42 DKMDLRFGFERLKEPGERTGWLINMHPTEILDED-KRLVSAVDYYFIQDDGSRFKVALPY 101
Query: 94 RPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSFDTV 153
PYFY A + + +V ++L ++++G+IA +E + KEDLDL NHL GL ++Y+KLSF TV
Sbjct: 102 MPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTV 161
Query: 154 QELMNVKSELLHVVESNREKSDAAEAY----DSILFG--------KREQRLQDFLECIVD 213
++L+ V+ E+ V+ NRE+ A++ Y SIL G + +++ D L+ IVD
Sbjct: 162 EDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKKIADQLDNIVD 221
Query: 214 LREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLE--KRKDLLQRAEVRICAFDIETTK 273
+REYDVPYH+R +ID + WY+V +E +R DL++R + + AFDIETTK
Sbjct: 222 MREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEITRRDDLVERPDPVVLAFDIETTK 281
Query: 274 LPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNE 333
LPLKFPDAE D IMMISYM+DGQGYLI NRE V EDIE+ E+TPKPEY+G F V N +E
Sbjct: 282 LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDE 341
Query: 334 LELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRS 393
+ L++ WF H++E KP I VTYNGD+FDWPF+E+RAA HGL M E+GFQ D +QGE ++
Sbjct: 342 VHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKD-SQGEYKA 401
Query: 394 KFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMKSEIVNNEVMQYL 453
H+DC WVKRDSYLP GSH
Sbjct: 402 PQCIHMDCLRWVKRDSYLPVGSH------------------------------------- 461
Query: 454 FNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDPLEVNPEDMVRFA 513
NL A KAKLGYDP+E++PEDM R A
Sbjct: 462 ----------------------------------NLKAAAKAKLGYDPVELDPEDMCRMA 521
Query: 514 KEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQ 573
E+PQ +A+YSVSDAVATYYLYM YVHPFIF+L TIIPM PDEVLRKGSGTLCE LLMVQ
Sbjct: 522 TEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQ 581
Query: 574 AYEANVVCPNKHQSDPEKF-HNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYD 633
A+ AN++ PNK + + K + +L++ETY+GGHVE LESGVFRSD+P FR++ +A+D
Sbjct: 582 AFHANIIFPNKQEQEFNKLTDDGHMLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFD 641
Query: 634 QLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGDEPMREECPLIY 693
L+ +++ +++AI+ E K+ +E +N+ +V I K+ + D P R ECPLIY
Sbjct: 642 FLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLK-----DVPNRIECPLIY 701
Query: 694 HLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRSFAGRFHFLTTL 753
HLDV AMYPNIILTNRLQ
Sbjct: 702 HLDVGAMYPNIILTNRLQ------------------------------------------ 761
Query: 754 RHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSCLIPAAVITDEV 813
P+A++ +
Sbjct: 762 ---------------------------------------------------PSAIVDEAT 821
Query: 814 CTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYHLKRQIESE-FV 873
C ACDFN+PG +C R++ W W GE A R +Y+ ++ Q+ESE F
Sbjct: 822 CAACDFNKPGASCQRKMAWQWRGEFMPASRS--------------EYHRIQHQLESEKFP 881
Query: 874 DSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYI 933
++ F EL + EQ K RL YC+KAYK++ V E R IC RENSFY+
Sbjct: 882 PLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEE-RLTTICQRENSFYV 941
Query: 934 DTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSF 993
DTVR L+KVWK KLS A G++ +++ ++M +LYDSLQLAHKCILNSF
Sbjct: 942 DTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSF 1001
Query: 994 YGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPEN 1053
YGYVMRKGARWYSMEMAG+V +TGA II AR LIE+IG+PLELDTDGIWC LP SFPEN
Sbjct: 1002 YGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPEN 1061
Query: 1054 FTFKTKESK--KLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDG 1113
F KT +K KLTISYP MLN+ V TN QYQ L +P Y SE SI FEVDG
Sbjct: 1062 FVIKTTNAKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTYVTHSENSIFFEVDG 1121
Query: 1114 PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQIYLYPYSSG 1173
PY AMILPASKEEG +KKRYAVFN+DG+LAELKGFE+KRRGEL+LIK+FQ
Sbjct: 1122 PYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQ--------- 1181
Query: 1174 MAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSKAELFDKFL 1233
+ +F+ FL
Sbjct: 1182 ----------------------------------------------------SSVFEAFL 1187
Query: 1234 HGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAV 1293
GSTLEE Y VA VA+ WLD+L S+ ++ DSEL + ISE+ +MS+ L DYGEQKS ++
Sbjct: 1242 KGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSI 1187
Query: 1294 TTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWC 1353
+TAKRLA+FLGD MV+D GL C+YI++ +P+G+PV+ERA+P+AIF+ +P + K ++RKW
Sbjct: 1302 STAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWL 1187
Query: 1354 KISS--EVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREK 1396
K SS + IR+I+DW YY +RL SAIQKIITIPAA+Q+V NPVPRV HPDWLHKK+ EK
Sbjct: 1362 KSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEK 1187
BLAST of Lsi02G017140 vs. ExPASy Swiss-Prot
Match:
Q4WXH8 (DNA polymerase epsilon catalytic subunit A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=pol2 PE=3 SV=1)
HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 634/1400 (45.29%), Postives = 820/1400 (58.57%), Query Frame = 0
Query: 36 IESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRP 95
I+ GF F G+KR+GWL+ S+S +D + + +D YF+ G SFK+ ++ P
Sbjct: 54 IDEALGFPRFESGEKRVGWLINMHSTSIEDPNVPGGRAGVDYYFLEDGGGSFKATVEYDP 113
Query: 96 YFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQE 155
YF A K E +VE + RR ++G I I+ +EKEDL L NHL G + +L+L+F V
Sbjct: 114 YFLLAVKKGHEAEVEEWCRRMFEGLIKTIKRVEKEDLQLPNHLLGHRRTFLQLNFANVSH 173
Query: 156 LMNVKSELLHVVESNREKSDAAEAY--------------DSILFGKREQRLQDFLECIVD 215
L++V+ LL + E N++ + + Y D ++ R + + I+D
Sbjct: 174 LLDVRKTLLPLAEKNKKNVNVMDTYAEISSANAGFDLFDDELINESRPNSNMNASDYIID 233
Query: 216 LREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICAFDIETTKLP 275
+REYDVPYHVR AID D+R G+WY V I+L ++ LQRA+ I AFDIETTKLP
Sbjct: 234 IREYDVPYHVRVAIDKDIRIGKWYTVEAKHGVISLTCLEERLQRADPVILAFDIETTKLP 293
Query: 276 LKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNELE 335
LKFPD+ D IMMISYM+DGQG+LI NRE V EDI + EYTPKPEY G F + N NE
Sbjct: 294 LKFPDSVIDQIMMISYMIDGQGFLITNREIVSEDIRDFEYTPKPEYSGPFMIFNEPNERS 353
Query: 336 LLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGEC-RSK 395
+++ +F H++E KP + TYNGD+FDWPF+E+RA+ G+ M E+GF+ KN + +S
Sbjct: 354 VIERFFEHIKEAKPTVIATYNGDFFDWPFVEARASVLGIDMYKEIGFR--KNSEDIYQSD 413
Query: 396 FACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMKSEIVNNEVMQYLF 455
H+DCFAWV RDSYLPQGS GLK
Sbjct: 414 HCVHMDCFAWVNRDSYLPQGSRGLK----------------------------------- 473
Query: 456 NIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDPLEVNPEDMVRFAK 515
AVT AKLGYDP E++PE M +A
Sbjct: 474 ------------------------------------AVTVAKLGYDPDELDPELMTPYAS 533
Query: 516 EKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQA 575
E+PQ +A YSVSDAVATYYLYM YVHPFIFSL TIIP++PD+ LRKG+GTLCEMLLMVQA
Sbjct: 534 ERPQTLAEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNPDDTLRKGTGTLCEMLLMVQA 593
Query: 576 YEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQL 635
Y+ +V PNKH+ PE F+ LLESETY+GGHVE +E+GVFRSD+P +F +D++A D+L
Sbjct: 594 YKGEIVLPNKHKDPPESFYEGHLLESETYVGGHVESIEAGVFRSDIPVTFNIDTTAIDEL 653
Query: 636 INNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGDEPMREECPLIYHL 695
+ +LD L+++I+VE K ++ V NY DVK I E++ + ++P R+E P IYHL
Sbjct: 654 LRDLDAALKFSIEVEEKKSMDDVVNYEDVKAQIAERLVNLR-----EKPHRDEVPSIYHL 713
Query: 696 DVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRSFAGRFHFLTTLRH 755
DVA+MYPNI++TNRLQ
Sbjct: 714 DVASMYPNIMITNRLQ-------------------------------------------- 773
Query: 756 VTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSCLIPAAVITDEVCT 815
P ++I + C
Sbjct: 774 -------------------------------------------------PDSMIQESDCA 833
Query: 816 ACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYHLKRQIESEFVDSG 875
ACDFNRPGKTC R L W W GE AKR D Y++ RQ + + G
Sbjct: 834 ACDFNRPGKTCDRRLPWAWRGEFLPAKR---------------DEYNMIRQAVANELFPG 893
Query: 876 TVRLS--KQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYID 935
+ S + F E+ EQ A +K+RL+ Y +K Y ++ D T VR+A IC REN FY+D
Sbjct: 894 RTKNSPMRSFGEMSAEEQAAIIKKRLQDYSKKIYHKIHDSK-TIVREAIICQRENPFYVD 953
Query: 936 TVR-----------LNKVWKGKLSEAKASG-NSIKIQEAQDMVVLYDSLQLAHKCILNSF 995
TVR KVWKGK KA+G ++ +I+EA+ M+VL+DSLQLAHK ILNSF
Sbjct: 954 TVRSFRDRRYDFKGKQKVWKGKTEALKAAGASAAEIEEAKKMIVLFDSLQLAHKVILNSF 1013
Query: 996 YGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPEN 1055
YGYVMRKG+RWYSMEMAGV TGA IIQ AR L+E+IG+PLELDTDGIWC LPGSFPE+
Sbjct: 1014 YGYVMRKGSRWYSMEMAGVTCLTGAHIIQMARELVERIGRPLELDTDGIWCMLPGSFPED 1073
Query: 1056 FTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPY 1115
F+F K KKL ISYPCVMLN V TN QYQTLVDP YE S+ SI FEVDGPY
Sbjct: 1074 FSFTLKNGKKLGISYPCVMLNHLVHGKYTNHQYQTLVDPKTFRYETHSDNSIFFEVDGPY 1133
Query: 1116 KAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQIYLYPYSSGMA 1175
KAMILP SKEE +KKRYAVFN DG+LAELKGFE+KRRGELKLIK+FQ
Sbjct: 1134 KAMILPTSKEEDKNLKKRYAVFNHDGSLAELKGFEVKRRGELKLIKIFQ----------- 1193
Query: 1176 KSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSKAELFDKFLHG 1235
++F FL G
Sbjct: 1194 --------------------------------------------------TQIFRFFLEG 1205
Query: 1236 STLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTT 1295
STLEE Y+ VA VA+RWLD+L G +AD EL++ ISE+ +M+K+L +YG QKS ++TT
Sbjct: 1254 STLEETYAAVARVADRWLDVLYDHGATLADEELIELISENRSMTKTLEEYGNQKSTSITT 1205
Query: 1296 AKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKI 1355
A+RLA+FLG+ MV+DKGL C+YI++ +PK TPV+ERA+PV IF + + +F++RKW K
Sbjct: 1314 ARRLAEFLGEQMVKDKGLNCKYIISAKPKNTPVTERAIPVTIFSAEEPVKRFFLRKWLKD 1205
Query: 1356 S-SEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDK 1406
++ RS+IDW YY +RL S +QK+ITIPAA+QKV NPVPRV HPDWL +++ K+DK
Sbjct: 1374 DPGDMDPRSVIDWDYYLERLGSVVQKLITIPAALQKVRNPVPRVAHPDWLQRRINMKEDK 1205
BLAST of Lsi02G017140 vs. ExPASy TrEMBL
Match:
A0A6J1C732 (DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111008850 PE=3 SV=1)
HSP 1 Score: 2157.9 bits (5590), Expect = 0.0e+00
Identity = 1127/1510 (74.64%), Postives = 1187/1510 (78.61%), Query Frame = 0
Query: 3 TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 62
T + +S +RRR+ D N+K KKQ I++S AEE+IESKFGF LFSEGDKRLGWLLTF
Sbjct: 17 TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76
Query: 63 SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 122
SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77 SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136
Query: 123 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 182
IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196
Query: 183 DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 242
DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197 DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256
Query: 243 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 302
DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316
Query: 303 YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 362
YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317 YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376
Query: 363 KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNC 422
KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 377 KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK--------------- 436
Query: 423 RGVNKMKSEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTK 482
AVTK
Sbjct: 437 --------------------------------------------------------AVTK 496
Query: 483 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSP 542
AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+P
Sbjct: 497 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTP 556
Query: 543 DEVLRKGSGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESG 602
DEVLRKGSGTLCEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESG
Sbjct: 557 DEVLRKGSGTLCEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESG 616
Query: 603 VFRSDLPTSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARI 662
VFRSD+PTSFRLDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK+ R+
Sbjct: 617 VFRSDIPTSFRLDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRL 676
Query: 663 SLFDCGDEPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLF 722
D P+REECPLIYHLDVAAMYPNIILTNRLQ
Sbjct: 677 R-----DGPVREECPLIYHLDVAAMYPNIILTNRLQ------------------------ 736
Query: 723 TVSHWRSFAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFM 782
Sbjct: 737 ------------------------------------------------------------ 796
Query: 783 ASTTYSCLIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFF 842
P +++TDEVCTACDFN P KTCLR+LEWVW GEVF AK+
Sbjct: 797 ---------PPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS----------- 856
Query: 843 HLFDYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPV 902
DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPV
Sbjct: 857 ---DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPV 916
Query: 903 TEVRKAGICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMV 962
TEVR+AGICMRENSFYIDTVR LNKVWKGKLSEAK SGNSIKIQEAQDM+
Sbjct: 917 TEVREAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDML 976
Query: 963 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1022
VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE
Sbjct: 977 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1036
Query: 1023 LDTDGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKI 1082
LDTDGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KI
Sbjct: 1037 LDTDGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKI 1096
Query: 1083 YEMRSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1142
Y+ SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK
Sbjct: 1097 YKTHSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1156
Query: 1143 LIKVFQIYLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEEL 1202
LIKVFQ
Sbjct: 1157 LIKVFQ------------------------------------------------------ 1216
Query: 1203 RNRSSSKAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1262
AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM
Sbjct: 1217 -------AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1276
Query: 1263 SKSLADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1322
SKSLADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF
Sbjct: 1277 SKSLADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1279
Query: 1323 ETDPEIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRV 1382
ETD EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRV
Sbjct: 1337 ETDAEIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRV 1279
Query: 1383 VHPDWLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQK 1442
VHPDWLHKKVREKDDKFRQQKIV+MFASS G N G S SK+VMN +NS+D+EDFQK
Sbjct: 1397 VHPDWLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQK 1279
Query: 1443 KTSSSSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNE 1500
KTSS +GPKPVVR YDVR MKHSV R+SQ+DS MQEK G PSSPL+F++IDRN
Sbjct: 1457 KTSSINGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNV 1279
BLAST of Lsi02G017140 vs. ExPASy TrEMBL
Match:
A0A6J1C6H8 (DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111008850 PE=3 SV=1)
HSP 1 Score: 2157.9 bits (5590), Expect = 0.0e+00
Identity = 1127/1510 (74.64%), Postives = 1187/1510 (78.61%), Query Frame = 0
Query: 3 TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 62
T + +S +RRR+ D N+K KKQ I++S AEE+IESKFGF LFSEGDKRLGWLLTF
Sbjct: 17 TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76
Query: 63 SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 122
SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77 SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136
Query: 123 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 182
IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196
Query: 183 DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 242
DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197 DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256
Query: 243 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 302
DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316
Query: 303 YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 362
YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317 YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376
Query: 363 KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNC 422
KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 377 KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK--------------- 436
Query: 423 RGVNKMKSEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTK 482
AVTK
Sbjct: 437 --------------------------------------------------------AVTK 496
Query: 483 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSP 542
AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+P
Sbjct: 497 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTP 556
Query: 543 DEVLRKGSGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESG 602
DEVLRKGSGTLCEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESG
Sbjct: 557 DEVLRKGSGTLCEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESG 616
Query: 603 VFRSDLPTSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARI 662
VFRSD+PTSFRLDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK+ R+
Sbjct: 617 VFRSDIPTSFRLDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRL 676
Query: 663 SLFDCGDEPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLF 722
D P+REECPLIYHLDVAAMYPNIILTNRLQ
Sbjct: 677 R-----DGPVREECPLIYHLDVAAMYPNIILTNRLQ------------------------ 736
Query: 723 TVSHWRSFAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFM 782
Sbjct: 737 ------------------------------------------------------------ 796
Query: 783 ASTTYSCLIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFF 842
P +++TDEVCTACDFN P KTCLR+LEWVW GEVF AK+
Sbjct: 797 ---------PPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS----------- 856
Query: 843 HLFDYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPV 902
DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPV
Sbjct: 857 ---DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPV 916
Query: 903 TEVRKAGICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMV 962
TEVR+AGICMRENSFYIDTVR LNKVWKGKLSEAK SGNSIKIQEAQDM+
Sbjct: 917 TEVREAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDML 976
Query: 963 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1022
VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE
Sbjct: 977 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1036
Query: 1023 LDTDGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKI 1082
LDTDGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KI
Sbjct: 1037 LDTDGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKI 1096
Query: 1083 YEMRSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1142
Y+ SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK
Sbjct: 1097 YKTHSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1156
Query: 1143 LIKVFQIYLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEEL 1202
LIKVFQ
Sbjct: 1157 LIKVFQ------------------------------------------------------ 1216
Query: 1203 RNRSSSKAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1262
AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM
Sbjct: 1217 -------AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1276
Query: 1263 SKSLADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1322
SKSLADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF
Sbjct: 1277 SKSLADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1279
Query: 1323 ETDPEIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRV 1382
ETD EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRV
Sbjct: 1337 ETDAEIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRV 1279
Query: 1383 VHPDWLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQK 1442
VHPDWLHKKVREKDDKFRQQKIV+MFASS G N G S SK+VMN +NS+D+EDFQK
Sbjct: 1397 VHPDWLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQK 1279
Query: 1443 KTSSSSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNE 1500
KTSS +GPKPVVR YDVR MKHSV R+SQ+DS MQEK G PSSPL+F++IDRN
Sbjct: 1457 KTSSINGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNV 1279
BLAST of Lsi02G017140 vs. ExPASy TrEMBL
Match:
A0A5A7SVB8 (DNA polymerase epsilon catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00410 PE=3 SV=1)
HSP 1 Score: 2142.5 bits (5550), Expect = 0.0e+00
Identity = 1114/1504 (74.07%), Postives = 1181/1504 (78.52%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S +RRR + + + KKQ +I+SAEE+IESK GF+LFSEGDKRLGWLLTF SSS +D+D
Sbjct: 2 SGESRRRGRAEKSSRSKKQRVIRSAEEEIESKLGFDLFSEGDKRLGWLLTFASSSLEDQD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62 TGKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGRIADIEIL 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL KAYLKLSFDTVQELMNVKSELLHVVE+N KSDAAEAYDSILFGK
Sbjct: 122 EKEDLDLKNHLSGLRKAYLKLSFDTVQELMNVKSELLHVVENNWAKSDAAEAYDSILFGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQ+ QDFLECI+DLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182 REQKPQDFLECIIDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDG GYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242 VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGNGYLIINRECVGEDIENLEYTPKPEY 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KMSDEVG
Sbjct: 302 EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMSDEVG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
FQCDKNQGECRSKFACHLDCF+WVKRDSYLPQGSHGLK
Sbjct: 362 FQCDKNQGECRSKFACHLDCFSWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG
Sbjct: 482 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMVQAY+ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLP
Sbjct: 542 SGTLCEMLLMVQAYKANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSFRLD SAYD LINNLDRDLQYAIQVEGKMDLE VSNYSDVK AI EK+ R+ D
Sbjct: 602 TSFRLDPSAYDLLINNLDRDLQYAIQVEGKMDLESVSNYSDVKNAIMEKLIRLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
EP+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 EPVREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P++++TDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+ DYYH
Sbjct: 782 --PSSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAG 907
LK+QIESEFVDSG VRLSK FLELPKLEQQAKLK+RLKKYCQKAYKRVLDKPVTEVR+AG
Sbjct: 842 LKKQIESEFVDSGNVRLSKSFLELPKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEVREAG 901
Query: 908 ICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 967
ICMRENSFYIDTVR LNKVWKGKLSEAK+SGNSIKIQEAQDMVVLYDSLQ
Sbjct: 902 ICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKSSGNSIKIQEAQDMVVLYDSLQ 961
Query: 968 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1027
LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW
Sbjct: 962 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1021
Query: 1028 CALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSEC 1087
CALPGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+ SEC
Sbjct: 1022 CALPGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSEC 1081
Query: 1088 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQI 1147
SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ- 1141
Query: 1148 YLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSK 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADY 1267
AELFDKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DY
Sbjct: 1202 AELFDKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDY 1259
Query: 1268 GEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIM 1327
GEQKSCAVTTA+RLADFLG+TMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIM
Sbjct: 1262 GEQKSCAVTTARRLADFLGETMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIM 1259
Query: 1328 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLH 1387
KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLH
Sbjct: 1322 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLH 1259
Query: 1388 KKVREKDDKFRQQKIVNMFA-SSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSS 1447
KKVREKDDKFRQQK+ +MFA SSR KY + N S SKN M+ NS+D+EDFQKKTSS++
Sbjct: 1382 KKVREKDDKFRQQKVADMFASSSRVKYSKKNSDVSCSKNAMDEDNSEDLEDFQKKTSSTN 1259
Query: 1448 GPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWL 1500
GPKPVVR YDV+ KHS+T D +++S MQ+ F + G +PSSPL ++ID+N DY GWL
Sbjct: 1442 GPKPVVRCYDVKGKKHSLTSDVEINSEMQDNFN--DGGFEPSSPLDVENIDKNVDYSGWL 1259
BLAST of Lsi02G017140 vs. ExPASy TrEMBL
Match:
A0A1S4E210 (DNA polymerase epsilon catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103497954 PE=3 SV=1)
HSP 1 Score: 2142.5 bits (5550), Expect = 0.0e+00
Identity = 1114/1504 (74.07%), Postives = 1181/1504 (78.52%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S +RRR + + + KKQ +I+SAEE+IESK GF+LFSEGDKRLGWLLTF SSS +D+D
Sbjct: 2 SGESRRRGRAEKSSRSKKQRVIRSAEEEIESKLGFDLFSEGDKRLGWLLTFASSSLEDQD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62 TGKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGRIADIEIL 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL KAYLKLSFDTVQELMNVKSELLHVVE+N KSDAAEAYDSILFGK
Sbjct: 122 EKEDLDLKNHLSGLRKAYLKLSFDTVQELMNVKSELLHVVENNWAKSDAAEAYDSILFGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQ+ QDFLECI+DLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182 REQKPQDFLECIIDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDG GYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242 VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGNGYLIINRECVGEDIENLEYTPKPEY 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KMSDEVG
Sbjct: 302 EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMSDEVG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
FQCDKNQGECRSKFACHLDCF+WVKRDSYLPQGSHGLK
Sbjct: 362 FQCDKNQGECRSKFACHLDCFSWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG
Sbjct: 482 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMVQAY+ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLP
Sbjct: 542 SGTLCEMLLMVQAYKANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSFRLD SAYD LINNLDRDLQYAIQVEGKMDLE VSNYSDVK AI EK+ R+ D
Sbjct: 602 TSFRLDPSAYDLLINNLDRDLQYAIQVEGKMDLESVSNYSDVKNAIMEKLIRLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
EP+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 EPVREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P++++TDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+ DYYH
Sbjct: 782 --PSSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAG 907
LK+QIESEFVDSG VRLSK FLELPKLEQQAKLK+RLKKYCQKAYKRVLDKPVTEVR+AG
Sbjct: 842 LKKQIESEFVDSGNVRLSKSFLELPKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEVREAG 901
Query: 908 ICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 967
ICMRENSFYIDTVR LNKVWKGKLSEAK+SGNSIKIQEAQDMVVLYDSLQ
Sbjct: 902 ICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKSSGNSIKIQEAQDMVVLYDSLQ 961
Query: 968 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1027
LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW
Sbjct: 962 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1021
Query: 1028 CALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSEC 1087
CALPGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+ SEC
Sbjct: 1022 CALPGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSEC 1081
Query: 1088 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQI 1147
SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ- 1141
Query: 1148 YLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSK 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADY 1267
AELFDKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DY
Sbjct: 1202 AELFDKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDY 1259
Query: 1268 GEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIM 1327
GEQKSCAVTTA+RLADFLG+TMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIM
Sbjct: 1262 GEQKSCAVTTARRLADFLGETMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIM 1259
Query: 1328 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLH 1387
KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLH
Sbjct: 1322 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLH 1259
Query: 1388 KKVREKDDKFRQQKIVNMFA-SSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSS 1447
KKVREKDDKFRQQK+ +MFA SSR KY + N S SKN M+ NS+D+EDFQKKTSS++
Sbjct: 1382 KKVREKDDKFRQQKVADMFASSSRVKYSKKNSDVSCSKNAMDEDNSEDLEDFQKKTSSTN 1259
Query: 1448 GPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWL 1500
GPKPVVR YDV+ KHS+T D +++S MQ+ F + G +PSSPL ++ID+N DY GWL
Sbjct: 1442 GPKPVVRCYDVKGKKHSLTSDVEINSEMQDNFN--DGGFEPSSPLDVENIDKNVDYSGWL 1259
BLAST of Lsi02G017140 vs. ExPASy TrEMBL
Match:
A0A1S3C879 (DNA polymerase epsilon catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103497954 PE=3 SV=1)
HSP 1 Score: 2142.5 bits (5550), Expect = 0.0e+00
Identity = 1114/1504 (74.07%), Postives = 1181/1504 (78.52%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S +RRR + + + KKQ +I+SAEE+IESK GF+LFSEGDKRLGWLLTF SSS +D+D
Sbjct: 2 SGESRRRGRAEKSSRSKKQRVIRSAEEEIESKLGFDLFSEGDKRLGWLLTFASSSLEDQD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62 TGKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGRIADIEIL 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL KAYLKLSFDTVQELMNVKSELLHVVE+N KSDAAEAYDSILFGK
Sbjct: 122 EKEDLDLKNHLSGLRKAYLKLSFDTVQELMNVKSELLHVVENNWAKSDAAEAYDSILFGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQ+ QDFLECI+DLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182 REQKPQDFLECIIDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDG GYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242 VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGNGYLIINRECVGEDIENLEYTPKPEY 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KMSDEVG
Sbjct: 302 EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMSDEVG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
FQCDKNQGECRSKFACHLDCF+WVKRDSYLPQGSHGLK
Sbjct: 362 FQCDKNQGECRSKFACHLDCFSWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG
Sbjct: 482 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMVQAY+ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLP
Sbjct: 542 SGTLCEMLLMVQAYKANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSFRLD SAYD LINNLDRDLQYAIQVEGKMDLE VSNYSDVK AI EK+ R+ D
Sbjct: 602 TSFRLDPSAYDLLINNLDRDLQYAIQVEGKMDLESVSNYSDVKNAIMEKLIRLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
EP+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 EPVREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P++++TDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+ DYYH
Sbjct: 782 --PSSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAG 907
LK+QIESEFVDSG VRLSK FLELPKLEQQAKLK+RLKKYCQKAYKRVLDKPVTEVR+AG
Sbjct: 842 LKKQIESEFVDSGNVRLSKSFLELPKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEVREAG 901
Query: 908 ICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 967
ICMRENSFYIDTVR LNKVWKGKLSEAK+SGNSIKIQEAQDMVVLYDSLQ
Sbjct: 902 ICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKSSGNSIKIQEAQDMVVLYDSLQ 961
Query: 968 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1027
LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW
Sbjct: 962 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1021
Query: 1028 CALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSEC 1087
CALPGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+ SEC
Sbjct: 1022 CALPGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSEC 1081
Query: 1088 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQI 1147
SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ- 1141
Query: 1148 YLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSK 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADY 1267
AELFDKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DY
Sbjct: 1202 AELFDKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDY 1259
Query: 1268 GEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIM 1327
GEQKSCAVTTA+RLADFLG+TMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIM
Sbjct: 1262 GEQKSCAVTTARRLADFLGETMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIM 1259
Query: 1328 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLH 1387
KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLH
Sbjct: 1322 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLH 1259
Query: 1388 KKVREKDDKFRQQKIVNMFA-SSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSS 1447
KKVREKDDKFRQQK+ +MFA SSR KY + N S SKN M+ NS+D+EDFQKKTSS++
Sbjct: 1382 KKVREKDDKFRQQKVADMFASSSRVKYSKKNSDVSCSKNAMDEDNSEDLEDFQKKTSSTN 1259
Query: 1448 GPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWL 1500
GPKPVVR YDV+ KHS+T D +++S MQ+ F + G +PSSPL ++ID+N DY GWL
Sbjct: 1442 GPKPVVRCYDVKGKKHSLTSDVEINSEMQDNFN--DGGFEPSSPLDVENIDKNVDYSGWL 1259
BLAST of Lsi02G017140 vs. NCBI nr
Match:
XP_022137385.1 (DNA polymerase epsilon catalytic subunit A-like isoform X2 [Momordica charantia])
HSP 1 Score: 2157.9 bits (5590), Expect = 0.0e+00
Identity = 1127/1510 (74.64%), Postives = 1187/1510 (78.61%), Query Frame = 0
Query: 3 TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 62
T + +S +RRR+ D N+K KKQ I++S AEE+IESKFGF LFSEGDKRLGWLLTF
Sbjct: 17 TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76
Query: 63 SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 122
SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77 SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136
Query: 123 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 182
IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196
Query: 183 DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 242
DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197 DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256
Query: 243 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 302
DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316
Query: 303 YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 362
YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317 YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376
Query: 363 KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNC 422
KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 377 KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK--------------- 436
Query: 423 RGVNKMKSEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTK 482
AVTK
Sbjct: 437 --------------------------------------------------------AVTK 496
Query: 483 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSP 542
AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+P
Sbjct: 497 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTP 556
Query: 543 DEVLRKGSGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESG 602
DEVLRKGSGTLCEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESG
Sbjct: 557 DEVLRKGSGTLCEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESG 616
Query: 603 VFRSDLPTSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARI 662
VFRSD+PTSFRLDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK+ R+
Sbjct: 617 VFRSDIPTSFRLDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRL 676
Query: 663 SLFDCGDEPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLF 722
D P+REECPLIYHLDVAAMYPNIILTNRLQ
Sbjct: 677 R-----DGPVREECPLIYHLDVAAMYPNIILTNRLQ------------------------ 736
Query: 723 TVSHWRSFAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFM 782
Sbjct: 737 ------------------------------------------------------------ 796
Query: 783 ASTTYSCLIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFF 842
P +++TDEVCTACDFN P KTCLR+LEWVW GEVF AK+
Sbjct: 797 ---------PPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS----------- 856
Query: 843 HLFDYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPV 902
DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPV
Sbjct: 857 ---DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPV 916
Query: 903 TEVRKAGICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMV 962
TEVR+AGICMRENSFYIDTVR LNKVWKGKLSEAK SGNSIKIQEAQDM+
Sbjct: 917 TEVREAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDML 976
Query: 963 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1022
VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE
Sbjct: 977 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1036
Query: 1023 LDTDGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKI 1082
LDTDGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KI
Sbjct: 1037 LDTDGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKI 1096
Query: 1083 YEMRSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1142
Y+ SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK
Sbjct: 1097 YKTHSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1156
Query: 1143 LIKVFQIYLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEEL 1202
LIKVFQ
Sbjct: 1157 LIKVFQ------------------------------------------------------ 1216
Query: 1203 RNRSSSKAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1262
AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM
Sbjct: 1217 -------AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1276
Query: 1263 SKSLADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1322
SKSLADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF
Sbjct: 1277 SKSLADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1279
Query: 1323 ETDPEIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRV 1382
ETD EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRV
Sbjct: 1337 ETDAEIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRV 1279
Query: 1383 VHPDWLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQK 1442
VHPDWLHKKVREKDDKFRQQKIV+MFASS G N G S SK+VMN +NS+D+EDFQK
Sbjct: 1397 VHPDWLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQK 1279
Query: 1443 KTSSSSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNE 1500
KTSS +GPKPVVR YDVR MKHSV R+SQ+DS MQEK G PSSPL+F++IDRN
Sbjct: 1457 KTSSINGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNV 1279
BLAST of Lsi02G017140 vs. NCBI nr
Match:
XP_022137384.1 (DNA polymerase epsilon catalytic subunit A-like isoform X1 [Momordica charantia])
HSP 1 Score: 2157.9 bits (5590), Expect = 0.0e+00
Identity = 1127/1510 (74.64%), Postives = 1187/1510 (78.61%), Query Frame = 0
Query: 3 TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 62
T + +S +RRR+ D N+K KKQ I++S AEE+IESKFGF LFSEGDKRLGWLLTF
Sbjct: 17 TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76
Query: 63 SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 122
SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77 SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136
Query: 123 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 182
IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137 IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196
Query: 183 DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 242
DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197 DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256
Query: 243 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 302
DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257 DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316
Query: 303 YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 362
YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317 YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376
Query: 363 KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNC 422
KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 377 KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK--------------- 436
Query: 423 RGVNKMKSEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTK 482
AVTK
Sbjct: 437 --------------------------------------------------------AVTK 496
Query: 483 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSP 542
AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+P
Sbjct: 497 AKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTP 556
Query: 543 DEVLRKGSGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESG 602
DEVLRKGSGTLCEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESG
Sbjct: 557 DEVLRKGSGTLCEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESG 616
Query: 603 VFRSDLPTSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARI 662
VFRSD+PTSFRLDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK+ R+
Sbjct: 617 VFRSDIPTSFRLDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRL 676
Query: 663 SLFDCGDEPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLF 722
D P+REECPLIYHLDVAAMYPNIILTNRLQ
Sbjct: 677 R-----DGPVREECPLIYHLDVAAMYPNIILTNRLQ------------------------ 736
Query: 723 TVSHWRSFAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFM 782
Sbjct: 737 ------------------------------------------------------------ 796
Query: 783 ASTTYSCLIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFF 842
P +++TDEVCTACDFN P KTCLR+LEWVW GEVF AK+
Sbjct: 797 ---------PPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS----------- 856
Query: 843 HLFDYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPV 902
DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPV
Sbjct: 857 ---DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPV 916
Query: 903 TEVRKAGICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMV 962
TEVR+AGICMRENSFYIDTVR LNKVWKGKLSEAK SGNSIKIQEAQDM+
Sbjct: 917 TEVREAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDML 976
Query: 963 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1022
VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE
Sbjct: 977 VLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLE 1036
Query: 1023 LDTDGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKI 1082
LDTDGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KI
Sbjct: 1037 LDTDGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKI 1096
Query: 1083 YEMRSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1142
Y+ SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK
Sbjct: 1097 YKTHSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELK 1156
Query: 1143 LIKVFQIYLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEEL 1202
LIKVFQ
Sbjct: 1157 LIKVFQ------------------------------------------------------ 1216
Query: 1203 RNRSSSKAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1262
AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM
Sbjct: 1217 -------AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTM 1276
Query: 1263 SKSLADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1322
SKSLADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF
Sbjct: 1277 SKSLADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIF 1279
Query: 1323 ETDPEIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRV 1382
ETD EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRV
Sbjct: 1337 ETDAEIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRV 1279
Query: 1383 VHPDWLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQK 1442
VHPDWLHKKVREKDDKFRQQKIV+MFASS G N G S SK+VMN +NS+D+EDFQK
Sbjct: 1397 VHPDWLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQK 1279
Query: 1443 KTSSSSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNE 1500
KTSS +GPKPVVR YDVR MKHSV R+SQ+DS MQEK G PSSPL+F++IDRN
Sbjct: 1457 KTSSINGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNV 1279
BLAST of Lsi02G017140 vs. NCBI nr
Match:
XP_038901114.1 (DNA polymerase epsilon catalytic subunit A-like isoform X1 [Benincasa hispida])
HSP 1 Score: 2146.7 bits (5561), Expect = 0.0e+00
Identity = 1121/1504 (74.53%), Postives = 1178/1504 (78.32%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S +RRR D + + KKQ II+SAEE+IESK GF+LFSEGDKRLGWLLTF SS +D+D
Sbjct: 2 SGESRRRGRTDKSSRSKKQRIIRSAEEEIESKLGFDLFSEGDKRLGWLLTFAPSSLEDQD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KV SCIDLYFVTQDGSSFKSKYKFRPYFY ATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62 TGKVNSCIDLYFVTQDGSSFKSKYKFRPYFYIATKDKMEIDVEAYLRRRYEGRIADIEIL 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL KAYLKLSFDTVQELMN+KSELLHVVESN KSDAAEAYDSILFGK
Sbjct: 122 EKEDLDLKNHLSGLCKAYLKLSFDTVQELMNLKSELLHVVESNWAKSDAAEAYDSILFGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182 REQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242 VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KM DEVG
Sbjct: 302 EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMIDEVG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 362 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG
Sbjct: 482 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMVQAY ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLP
Sbjct: 542 SGTLCEMLLMVQAYNANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSFRLD SAYDQLI NLDRDLQYAIQVEGKMDLELVSNYSDVK AI EK+ R+ D
Sbjct: 602 TSFRLDPSAYDQLIYNLDRDLQYAIQVEGKMDLELVSNYSDVKNAILEKLIRLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
EP+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 EPVREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P +++TDE+CTACDFNRPGKTCLR+LEWVW GEVF AK+ DYYH
Sbjct: 782 --PPSIVTDEICTACDFNRPGKTCLRKLEWVWRGEVFMAKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAG 907
LK+QIESEFVDSG VRLSK FLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVR+AG
Sbjct: 842 LKKQIESEFVDSGNVRLSKSFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVREAG 901
Query: 908 ICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 967
ICMRENSFYIDTVR LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ
Sbjct: 902 ICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 961
Query: 968 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1027
LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW
Sbjct: 962 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1021
Query: 1028 CALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSEC 1087
CALPGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+ SEC
Sbjct: 1022 CALPGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSEC 1081
Query: 1088 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQI 1147
SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ- 1141
Query: 1148 YLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSK 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADY 1267
AELFDKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DY
Sbjct: 1202 AELFDKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDY 1259
Query: 1268 GEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIM 1327
GEQKSCAVTTA+RLADFLGDTMV+DKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIM
Sbjct: 1262 GEQKSCAVTTARRLADFLGDTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIM 1259
Query: 1328 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLH 1387
KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLH
Sbjct: 1322 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLH 1259
Query: 1388 KKVREKDDKFRQQKIVNMFASS-RGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSS 1447
KKVREKDDKFRQQK+ +MFASS R KY + N S SK+ MN NS+D+EDFQKKTS +
Sbjct: 1382 KKVREKDDKFRQQKVADMFASSNRVKYSKKNSDVSCSKHAMNEDNSEDLEDFQKKTSPRN 1259
Query: 1448 GPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWL 1500
GPKPVVR YDVR MKHS+T +S+++S MQE F + G +PSSPLH ++ID+N DY GWL
Sbjct: 1442 GPKPVVRCYDVRGMKHSLTSNSEINSEMQENFN--DGGCEPSSPLHVENIDKNVDYSGWL 1259
BLAST of Lsi02G017140 vs. NCBI nr
Match:
XP_038901115.1 (DNA polymerase epsilon catalytic subunit A-like isoform X2 [Benincasa hispida])
HSP 1 Score: 2146.7 bits (5561), Expect = 0.0e+00
Identity = 1121/1504 (74.53%), Postives = 1178/1504 (78.32%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S +RRR D + + KKQ II+SAEE+IESK GF+LFSEGDKRLGWLLTF SS +D+D
Sbjct: 2 SGESRRRGRTDKSSRSKKQRIIRSAEEEIESKLGFDLFSEGDKRLGWLLTFAPSSLEDQD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KV SCIDLYFVTQDGSSFKSKYKFRPYFY ATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62 TGKVNSCIDLYFVTQDGSSFKSKYKFRPYFYIATKDKMEIDVEAYLRRRYEGRIADIEIL 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL KAYLKLSFDTVQELMN+KSELLHVVESN KSDAAEAYDSILFGK
Sbjct: 122 EKEDLDLKNHLSGLCKAYLKLSFDTVQELMNLKSELLHVVESNWAKSDAAEAYDSILFGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182 REQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242 VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KM DEVG
Sbjct: 302 EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMIDEVG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 362 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG
Sbjct: 482 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMVQAY ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLP
Sbjct: 542 SGTLCEMLLMVQAYNANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSFRLD SAYDQLI NLDRDLQYAIQVEGKMDLELVSNYSDVK AI EK+ R+ D
Sbjct: 602 TSFRLDPSAYDQLIYNLDRDLQYAIQVEGKMDLELVSNYSDVKNAILEKLIRLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
EP+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 EPVREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P +++TDE+CTACDFNRPGKTCLR+LEWVW GEVF AK+ DYYH
Sbjct: 782 --PPSIVTDEICTACDFNRPGKTCLRKLEWVWRGEVFMAKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAG 907
LK+QIESEFVDSG VRLSK FLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVR+AG
Sbjct: 842 LKKQIESEFVDSGNVRLSKSFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVREAG 901
Query: 908 ICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 967
ICMRENSFYIDTVR LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ
Sbjct: 902 ICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 961
Query: 968 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1027
LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW
Sbjct: 962 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1021
Query: 1028 CALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSEC 1087
CALPGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+ SEC
Sbjct: 1022 CALPGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSEC 1081
Query: 1088 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQI 1147
SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ- 1141
Query: 1148 YLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSK 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADY 1267
AELFDKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DY
Sbjct: 1202 AELFDKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDY 1259
Query: 1268 GEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIM 1327
GEQKSCAVTTA+RLADFLGDTMV+DKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIM
Sbjct: 1262 GEQKSCAVTTARRLADFLGDTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIM 1259
Query: 1328 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLH 1387
KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLH
Sbjct: 1322 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLH 1259
Query: 1388 KKVREKDDKFRQQKIVNMFASS-RGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSS 1447
KKVREKDDKFRQQK+ +MFASS R KY + N S SK+ MN NS+D+EDFQKKTS +
Sbjct: 1382 KKVREKDDKFRQQKVADMFASSNRVKYSKKNSDVSCSKHAMNEDNSEDLEDFQKKTSPRN 1259
Query: 1448 GPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWL 1500
GPKPVVR YDVR MKHS+T +S+++S MQE F + G +PSSPLH ++ID+N DY GWL
Sbjct: 1442 GPKPVVRCYDVRGMKHSLTSNSEINSEMQENFN--DGGCEPSSPLHVENIDKNVDYSGWL 1259
BLAST of Lsi02G017140 vs. NCBI nr
Match:
KAA0033391.1 (DNA polymerase epsilon catalytic subunit A-like isoform X1 [Cucumis melo var. makuwa])
HSP 1 Score: 2142.5 bits (5550), Expect = 0.0e+00
Identity = 1114/1504 (74.07%), Postives = 1181/1504 (78.52%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S +RRR + + + KKQ +I+SAEE+IESK GF+LFSEGDKRLGWLLTF SSS +D+D
Sbjct: 2 SGESRRRGRAEKSSRSKKQRVIRSAEEEIESKLGFDLFSEGDKRLGWLLTFASSSLEDQD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62 TGKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGRIADIEIL 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL KAYLKLSFDTVQELMNVKSELLHVVE+N KSDAAEAYDSILFGK
Sbjct: 122 EKEDLDLKNHLSGLRKAYLKLSFDTVQELMNVKSELLHVVENNWAKSDAAEAYDSILFGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQ+ QDFLECI+DLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182 REQKPQDFLECIIDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDG GYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242 VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGNGYLIINRECVGEDIENLEYTPKPEY 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KMSDEVG
Sbjct: 302 EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMSDEVG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
FQCDKNQGECRSKFACHLDCF+WVKRDSYLPQGSHGLK
Sbjct: 362 FQCDKNQGECRSKFACHLDCFSWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG
Sbjct: 482 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMVQAY+ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLP
Sbjct: 542 SGTLCEMLLMVQAYKANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSFRLD SAYD LINNLDRDLQYAIQVEGKMDLE VSNYSDVK AI EK+ R+ D
Sbjct: 602 TSFRLDPSAYDLLINNLDRDLQYAIQVEGKMDLESVSNYSDVKNAIMEKLIRLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
EP+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 EPVREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P++++TDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+ DYYH
Sbjct: 782 --PSSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAG 907
LK+QIESEFVDSG VRLSK FLELPKLEQQAKLK+RLKKYCQKAYKRVLDKPVTEVR+AG
Sbjct: 842 LKKQIESEFVDSGNVRLSKSFLELPKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEVREAG 901
Query: 908 ICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQ 967
ICMRENSFYIDTVR LNKVWKGKLSEAK+SGNSIKIQEAQDMVVLYDSLQ
Sbjct: 902 ICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKSSGNSIKIQEAQDMVVLYDSLQ 961
Query: 968 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1027
LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW
Sbjct: 962 LAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIW 1021
Query: 1028 CALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSEC 1087
CALPGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+ SEC
Sbjct: 1022 CALPGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSEC 1081
Query: 1088 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQI 1147
SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 SIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ- 1141
Query: 1148 YLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSK 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 AELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADY 1267
AELFDKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DY
Sbjct: 1202 AELFDKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDY 1259
Query: 1268 GEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIM 1327
GEQKSCAVTTA+RLADFLG+TMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIM
Sbjct: 1262 GEQKSCAVTTARRLADFLGETMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIM 1259
Query: 1328 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLH 1387
KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLH
Sbjct: 1322 KFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLH 1259
Query: 1388 KKVREKDDKFRQQKIVNMFA-SSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSS 1447
KKVREKDDKFRQQK+ +MFA SSR KY + N S SKN M+ NS+D+EDFQKKTSS++
Sbjct: 1382 KKVREKDDKFRQQKVADMFASSSRVKYSKKNSDVSCSKNAMDEDNSEDLEDFQKKTSSTN 1259
Query: 1448 GPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWL 1500
GPKPVVR YDV+ KHS+T D +++S MQ+ F + G +PSSPL ++ID+N DY GWL
Sbjct: 1442 GPKPVVRCYDVKGKKHSLTSDVEINSEMQDNFN--DGGFEPSSPLDVENIDKNVDYSGWL 1259
BLAST of Lsi02G017140 vs. TAIR 10
Match:
AT1G08260.1 (DNA polymerase epsilon catalytic subunit )
HSP 1 Score: 1895.9 bits (4910), Expect = 0.0e+00
Identity = 985/1505 (65.45%), Postives = 1114/1505 (74.02%), Query Frame = 0
Query: 8 SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 67
S NRRRD +D + KK ++ +AE+++ESK GF LFSEG+ RLGWLLTF+SSS +D D
Sbjct: 2 SGDNRRRDRKDTRWS-KKPKVVNTAEDELESKLGFGLFSEGETRLGWLLTFSSSSWEDRD 61
Query: 68 TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 127
T KVYSC+DLYFVTQDG SFK+KYKFRPYFYAATK+KME ++EAYLRRRY+ Q+ADIEI+
Sbjct: 62 TGKVYSCVDLYFVTQDGFSFKTKYKFRPYFYAATKDKMELELEAYLRRRYERQVADIEIV 121
Query: 128 EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 187
EKEDLDLKNHLSGL K YLK+SFDTVQ+LM VK +LLH+VE N+ K DA EAY+SIL GK
Sbjct: 122 EKEDLDLKNHLSGLQKKYLKISFDTVQQLMEVKRDLLHIVERNQAKFDALEAYESILAGK 181
Query: 188 REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 247
REQR QD L+ IVDLREYDVPYHVRFAID+D+R GQWY+VS+SST + LEKR DLLQRAE
Sbjct: 182 REQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAE 241
Query: 248 VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 307
VR+CAFDIETTKLPLKFPDAEYD IMMISYMVDGQG+LIINRECVGED+E+LEYTPKPE+
Sbjct: 242 VRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQGFLIINRECVGEDVEDLEYTPKPEF 301
Query: 308 QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 367
+G+FKV NVKNE+ELL+ WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+G
Sbjct: 302 EGYFKVTNVKNEVELLQRWFYHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELG 361
Query: 368 FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMK 427
F+CD+NQGECR+KFACHLDCFAWVKRDSYLPQGSHGLK
Sbjct: 362 FRCDQNQGECRAKFACHLDCFAWVKRDSYLPQGSHGLK---------------------- 421
Query: 428 SEIVNNEVMQYLFNIVIAPLSAPVSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDP 487
AVTKAKLGYDP
Sbjct: 422 -------------------------------------------------AVTKAKLGYDP 481
Query: 488 LEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKG 547
LEVNPEDMVRFA EKPQ MASYSVSDAVATYYLYMTYV+PFIFSLATIIPM PDEVLRKG
Sbjct: 482 LEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVNPFIFSLATIIPMVPDEVLRKG 541
Query: 548 SGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLP 607
SGTLCEMLLMV+AY+ANVVCPNK+Q+DPEKF+ N+LLESETYIGGHVECLESGVFRSD+P
Sbjct: 542 SGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQNQLLESETYIGGHVECLESGVFRSDIP 601
Query: 608 TSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGD 667
TSF+LDSSAY QLI+NL RDL+YAI VEGKM ++ +SNY +VK I EK+ ++ D
Sbjct: 602 TSFKLDSSAYQQLIDNLGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLR-----D 661
Query: 668 EPMREECPLIYHLDVAAMYPNIILTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRS 727
+P+REE PLIYHLDVAAMYPNIILTNRLQ
Sbjct: 662 DPIREEGPLIYHLDVAAMYPNIILTNRLQ------------------------------- 721
Query: 728 FAGRFHFLTTLRHVTTYKSIEVTLGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSC 787
Sbjct: 722 ------------------------------------------------------------ 781
Query: 788 LIPAAVITDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYH 847
P +++TDE+CTACDFNRPGKTCLR+LEWVW G F K+ DYYH
Sbjct: 782 --PPSIVTDEICTACDFNRPGKTCLRKLEWVWRGVTFMGKKS--------------DYYH 841
Query: 848 LKRQIESEFVDSG-TVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKA 907
LK+QIESEFVD+G + SK FL+LPK++QQ+KLKERLKKYCQKAYKRVLDKP+TEVR+A
Sbjct: 842 LKKQIESEFVDAGANIMSSKSFLDLPKVDQQSKLKERLKKYCQKAYKRVLDKPITEVREA 901
Query: 908 GICMRENSFYIDTVR-----------LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSL 967
GICMREN FY+DTVR LNKVWKGKLSEAKASGNSIKIQEAQDMVV+YDSL
Sbjct: 902 GICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNSIKIQEAQDMVVVYDSL 961
Query: 968 QLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGI 1027
QLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGI
Sbjct: 962 QLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGI 1021
Query: 1028 WCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSE 1087
WC LPGSFPENFTFKT + KKLTISYPCVMLNVDVA+NNTNDQYQTLVDPVRK Y+ SE
Sbjct: 1022 WCCLPGSFPENFTFKTIDMKKLTISYPCVMLNVDVAKNNTNDQYQTLVDPVRKTYKSHSE 1081
Query: 1088 CSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQ 1147
CSIEFEVDGPYKAMI+PASKEEGILIKKRYAVFN DGTLAELKGFEIKRRGELKLIKVFQ
Sbjct: 1082 CSIEFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTLAELKGFEIKRRGELKLIKVFQ 1141
Query: 1148 IYLYPYSSGMAKSYNIDAHADMHPKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSS 1207
Sbjct: 1142 ------------------------------------------------------------ 1201
Query: 1208 KAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLAD 1267
AELFDKFLHGSTLEECYS VA+VA+RWLDLLD+QGKDIADSELLDYISESSTMSKSLAD
Sbjct: 1202 -AELFDKFLHGSTLEECYSAVAAVADRWLDLLDNQGKDIADSELLDYISESSTMSKSLAD 1241
Query: 1268 YGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEI 1327
YGEQKSCAVTTAKRLA+FLG TMV+DKGLRCQYIVACEPKGTPVSERAVPVAIF T+PE+
Sbjct: 1262 YGEQKSCAVTTAKRLAEFLGVTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFTTNPEV 1241
Query: 1328 MKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWL 1387
MKF++RKWCK SS+VGIR IIDWSYYKQRLSSAIQK+ITIPAAMQKV+NPVPRV+HPDWL
Sbjct: 1322 MKFHLRKWCKTSSDVGIRLIIDWSYYKQRLSSAIQKVITIPAAMQKVANPVPRVLHPDWL 1241
Query: 1388 HKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSS- 1447
HKKVREKDDKFRQ+K+V+MF+S+ N D + + N +DIEDF K+ S
Sbjct: 1382 HKKVREKDDKFRQRKLVDMFSSA------NKDVVLDTDLPVTKDNVEDIEDFCKENRPSV 1241
Query: 1448 SGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGW 1500
GPKP+ RSY+V + Q + + ES + F++ID++ +Y GW
Sbjct: 1442 KGPKPIARSYEV--------------NKKQSECEQQESWDTEFHDISFQNIDKSVNYQGW 1241
BLAST of Lsi02G017140 vs. TAIR 10
Match:
AT2G27120.1 (DNA polymerase epsilon catalytic subunit )
HSP 1 Score: 1786.5 bits (4626), Expect = 0.0e+00
Identity = 937/1482 (63.23%), Postives = 1069/1482 (72.13%), Query Frame = 0
Query: 31 SAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSK 90
SA +++E+K GF LFS+G+ RLGWLLTF SSS +D DT K +SC+DL+FVTQDGSSFK+K
Sbjct: 16 SAADELETKLGFGLFSQGETRLGWLLTFASSSWEDADTGKTFSCVDLFFVTQDGSSFKTK 75
Query: 91 YKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSF 150
YKFRPY YAATK+ ME +VEAYLRRRY+ Q+ADI+I+ KEDL LKNHLSGL K YLK+SF
Sbjct: 76 YKFRPYLYAATKDNMELEVEAYLRRRYERQVADIQIVHKEDLYLKNHLSGLQKKYLKVSF 135
Query: 151 DTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRLQDFLECIVDLREYDVPYH 210
DTVQ+L+ VK +LLH+VE N K +A EAY+SIL GKREQR QD L+ +VDLREYDVPYH
Sbjct: 136 DTVQQLVEVKRDLLHIVERNLAKFNALEAYESILSGKREQRPQDCLDSVVDLREYDVPYH 195
Query: 211 VRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICAFDIETTKLPLKFPDAEYD 270
VRFAID+D+R GQWY+VS+SST + LEKR DLLQRAEVR+CAFDIET KLPLKFPDAEYD
Sbjct: 196 VRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETVKLPLKFPDAEYD 255
Query: 271 MIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNELELLKLWFAHM 330
IMMISYMVDGQG+LI NRECVG+DIE+LEYTPKPE++G+FKV NV NE+ELL+ WF+HM
Sbjct: 256 QIMMISYMVDGQGFLITNRECVGKDIEDLEYTPKPEFEGYFKVTNVTNEVELLRKWFSHM 315
Query: 331 REVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRSKFACHLDCFAW 390
+E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+GF+CD+NQGECR+KF CHLDCF+W
Sbjct: 316 QELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFVCHLDCFSW 375
Query: 391 VKRDSYLPQGSHGLKLPSFFLVFCGFCLNCRGVNKMKSEIVNNEVMQYLFNIVIAPLSAP 450
VKRDSYLPQGS GLK
Sbjct: 376 VKRDSYLPQGSQGLK--------------------------------------------- 435
Query: 451 VSISLSLERVRAEGNADADIYCFNLMAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYS 510
AVTK KLGYDPLEVNPEDMVRFA EKPQ MASYS
Sbjct: 436 --------------------------AVTKVKLGYDPLEVNPEDMVRFAMEKPQTMASYS 495
Query: 511 VSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNK 570
VSDAVATYYLYMTYVHPF+FSLATIIPM PDEVLRKGSGTLCEMLLMV+AY+ANVVCPNK
Sbjct: 496 VSDAVATYYLYMTYVHPFVFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNK 555
Query: 571 HQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINNLDRDLQY 630
+Q+DPEKF+ +LLESETYIGGHVECL+SGVFRSD+PTSF+LD+SAY QLI+NL RDL+Y
Sbjct: 556 NQADPEKFYQGKLLESETYIGGHVECLQSGVFRSDIPTSFKLDASAYQQLIDNLGRDLEY 615
Query: 631 AIQVEGKMDLELVSNYSDVKTAITEKVARISLFDCGDEPMREECPLIYHLDVAAMYPNII 690
AI VEGKM ++ VSN+ +VK I EK+ ++ D+P+REE PLIYHLDVAAMYPNII
Sbjct: 616 AITVEGKMRMDSVSNFDEVKEVIREKLEKLR-----DDPIREEGPLIYHLDVAAMYPNII 675
Query: 691 LTNRLQVQQTFFSLLVEEYLIGLCFEPRLFTVSHWRSFAGRFHFLTTLRHVTTYKSIEVT 750
LTNRLQ
Sbjct: 676 LTNRLQ------------------------------------------------------ 735
Query: 751 LGQFKPSVKLLLPKIILFLNVLSISYMFFMASTTYSCLIPAAVITDEVCTACDFNRPGKT 810
P +++TDEVCTACDFN P KT
Sbjct: 736 ---------------------------------------PPSIVTDEVCTACDFNGPEKT 795
Query: 811 CLRELEWVWHGEVFEAKRRSTLSLSLLVFFHLFDYYHLKRQIESEFVDSG-TVRLSKQFL 870
CLR+LEWVW G F+ + +YYHLK+QIESE VD+G ++ SK FL
Sbjct: 796 CLRKLEWVWRGVTFKGNKS--------------EYYHLKKQIESESVDAGANMQSSKPFL 855
Query: 871 ELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYIDTVR-------- 930
+LPK+EQQ+KLKERLKKYCQKAY RVLDKP+TEVR+AGICMREN FY+DTVR
Sbjct: 856 DLPKVEQQSKLKERLKKYCQKAYSRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYE 915
Query: 931 ---LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWY 990
LNKVWKGKLSEAKASGN IKIQEA DMVV+YDSLQLAHKCILNSFYGYVMRKGARWY
Sbjct: 916 YKTLNKVWKGKLSEAKASGNLIKIQEAHDMVVVYDSLQLAHKCILNSFYGYVMRKGARWY 975
Query: 991 SMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPENFTFKTKESKKLT 1050
SMEMAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGIWCALPGSFPENFTFKT + KK T
Sbjct: 976 SMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCALPGSFPENFTFKTIDMKKFT 1035
Query: 1051 ISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPYKAMILPASKEEG 1110
ISYPCV+LNVDVA+NN+NDQYQTLVDPVRK Y RSECSIEFEVDGPYKAMI+PASKEEG
Sbjct: 1036 ISYPCVILNVDVAKNNSNDQYQTLVDPVRKTYNSRSECSIEFEVDGPYKAMIIPASKEEG 1095
Query: 1111 ILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQIYLYPYSSGMAKSYNIDAHADMH 1170
ILIKKRYAVFN DGT+AELKGFE+KRRGELKLIKVFQ
Sbjct: 1096 ILIKKRYAVFNHDGTIAELKGFEMKRRGELKLIKVFQ----------------------- 1155
Query: 1171 PKLLFGGLVNVKIIMNLKLPFSSIRFWKEELRNRSSSKAELFDKFLHGSTLEECYSVVAS 1230
AELFDKFLHGSTLEECYS VA+
Sbjct: 1156 --------------------------------------AELFDKFLHGSTLEECYSAVAA 1215
Query: 1231 VANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAKRLADFLGDTM 1290
VANRWLDLL+ QGKDIADSELLDYISESSTMSKSLADYG+QKSCAVTTAKRLADFLGDTM
Sbjct: 1216 VANRWLDLLEGQGKDIADSELLDYISESSTMSKSLADYGQQKSCAVTTAKRLADFLGDTM 1225
Query: 1291 VRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKISSEVGIRSIIDW 1350
V+DKGLRCQYIVA EP+GTPVSERAVPVAIF+TD KFY++KWCKISS GIRSIIDW
Sbjct: 1276 VKDKGLRCQYIVAREPEGTPVSERAVPVAIFQTDDPEKKFYLQKWCKISSYTGIRSIIDW 1225
Query: 1351 SYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDKFRQQKIVNMFASS 1410
YYKQRL SAIQK+ITIPAAMQKV+NPV RV HP WL KKV DKFRQ KIV+MF+S+
Sbjct: 1336 MYYKQRLHSAIQKVITIPAAMQKVANPVLRVRHPYWLEKKV---CDKFRQGKIVDMFSSA 1225
Query: 1411 RGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSS-SGPKPVVRSYDVRAMKHSVTRDS 1470
N D +++ NV+ DIE+F K+ S GPKPV RS++V HS
Sbjct: 1396 ------NKDHSTTQDNVV-----ADIEEFCKENRPSVKGPKPVARSFEV-DRNHS----- 1225
Query: 1471 QVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1500
+ K ES + +++D+N DY GWLE++K
Sbjct: 1456 --------EGKQQESWDPEFHDISLQNVDKNVDYQGWLELEK 1225
BLAST of Lsi02G017140 vs. TAIR 10
Match:
AT5G63960.1 (DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases;DNA-directed DNA polymerases )
HSP 1 Score: 54.3 bits (129), Expect = 9.8e-07
Identity = 72/318 (22.64%), Postives = 124/318 (38.99%), Query Frame = 0
Query: 75 IDLYFVTQDGSSFKS-KYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLD 134
I ++ VT++G+S + F PYFY A M D + + +G++ + K
Sbjct: 124 IRMFGVTREGNSVCCFVHGFEPYFYIACPPGMGPDDISNFHQSLEGRMRESNKNAKVPKF 183
Query: 135 LKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRLQ 194
+K ++ + Q + + L +V S R D D G + Q
Sbjct: 184 VKRIEMVQKRSIMYYQQQKSQTFLKITVALPTMVASCRGILDRGLQID----GLGMKSFQ 243
Query: 195 DFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVS--------------------STG 254
+ E ++ + +RF +D D+ G W +V S
Sbjct: 244 TY--------ESNILFVLRFMVDCDIVGGNWIEVPTGKYKKNARTLSYCQLEFHCLYSDL 303
Query: 255 ITLEKRKDLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQG-------YLI 314
I+ + + A R+ +FDIE FP+A++D ++ I+ +V QG ++
Sbjct: 304 ISHAAEGEYSKMAPFRVLSFDIECAGRKGHFPEAKHDPVIQIANLVTLQGEDHPFVRNVM 363
Query: 315 INREC---VGEDIENLEYTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNG 362
+ C VG D+ + E E E+L W +R+V P I + YN
Sbjct: 364 TLKSCAPIVGVDVMSFE-----------------TEREVLLAWRDLIRDVDPDIIIGYNI 412
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4HW04 | 0.0e+00 | 65.45 | DNA polymerase epsilon catalytic subunit A OS=Arabidopsis thaliana OX=3702 GN=PO... | [more] |
F4IFN6 | 0.0e+00 | 63.23 | DNA polymerase epsilon catalytic subunit B OS=Arabidopsis thaliana OX=3702 GN=PO... | [more] |
Q07864 | 0.0e+00 | 45.68 | DNA polymerase epsilon catalytic subunit A OS=Homo sapiens OX=9606 GN=POLE PE=1 ... | [more] |
Q9WVF7 | 0.0e+00 | 46.09 | DNA polymerase epsilon catalytic subunit A OS=Mus musculus OX=10090 GN=Pole PE=1... | [more] |
Q4WXH8 | 0.0e+00 | 45.29 | DNA polymerase epsilon catalytic subunit A OS=Neosartorya fumigata (strain ATCC ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C732 | 0.0e+00 | 74.64 | DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A6J1C6H8 | 0.0e+00 | 74.64 | DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A5A7SVB8 | 0.0e+00 | 74.07 | DNA polymerase epsilon catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 ... | [more] |
A0A1S4E210 | 0.0e+00 | 74.07 | DNA polymerase epsilon catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103497954... | [more] |
A0A1S3C879 | 0.0e+00 | 74.07 | DNA polymerase epsilon catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103497954... | [more] |
Match Name | E-value | Identity | Description | |
XP_022137385.1 | 0.0e+00 | 74.64 | DNA polymerase epsilon catalytic subunit A-like isoform X2 [Momordica charantia] | [more] |
XP_022137384.1 | 0.0e+00 | 74.64 | DNA polymerase epsilon catalytic subunit A-like isoform X1 [Momordica charantia] | [more] |
XP_038901114.1 | 0.0e+00 | 74.53 | DNA polymerase epsilon catalytic subunit A-like isoform X1 [Benincasa hispida] | [more] |
XP_038901115.1 | 0.0e+00 | 74.53 | DNA polymerase epsilon catalytic subunit A-like isoform X2 [Benincasa hispida] | [more] |
KAA0033391.1 | 0.0e+00 | 74.07 | DNA polymerase epsilon catalytic subunit A-like isoform X1 [Cucumis melo var. ma... | [more] |
Match Name | E-value | Identity | Description | |
AT1G08260.1 | 0.0e+00 | 65.45 | DNA polymerase epsilon catalytic subunit | [more] |
AT2G27120.1 | 0.0e+00 | 63.23 | DNA polymerase epsilon catalytic subunit | [more] |
AT5G63960.1 | 9.8e-07 | 22.64 | DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases... | [more] |