Lsi02G015340 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G015340
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Metalloendoproteinase 3-MMP-like
Location	chr02: 21203172 .. 21204062 (-)
RNA-Seq Expression	Lsi02G015340
Synteny	Lsi02G015340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTAAATTATGTCTCCAACTCTTCCTACTTCTTGCCTTCATTACACCTCACTTGACCTCAACCCGCCCTCTTCACAACCACAAATCATCTCGACTTCTATTTCCCAAGCATCTTCAAGGAAGTCGTAAAGACGATAACATCGAAGGAATCCATAAGGTCAAGGCTTACCTTCAACGTTATGGTTACTTAAGCAATAAAAGCGAAAACAAATTGAATACAGACTCATTCGACGAGGACTTGGAATCCGCCATTAAATCATACCAAACGTTCTTCAAGCTTAATGTGAGTGGAGTCTTAGACAAGGAGACCCTACAACAAATGTCGAAGCCTCGATGTGGGGTGACAGACAATTTCATAGTATCACAAGGTAAGAATGTGGAAATGAATGGTTCTCATTATTCATTCTTCCCAAAGAAACGAACATGGCCAGTTGAGAAGAAGCATTTGACGTACGGATTTATCCACAATTACCCACCAGAGCACGTGGCTGCAGTGATGCGAGCTTTAGAGACATGGGCAGATAATACGAAATTCACCTTTGCCCAGGCTGCAAGGGTACAATCGGCAGACATTTTGTTAAGCTTTGAGAGAGGAGACCATGGAGATGGGCGTCCGTTTGATGGAGAAGGGGGAGTATTGGCACATGCATTTGGACCAATAGATGGGAGAATACATTTTGATGCGGATGAGCCATGGGAAAGAGGTCCTATTTTGGAACAGTTTGATGTGGAGTCGGTGGCATTGCATGAGTTGGGACATGCACTTGGGCTTGGCCATAGCAACATTGAATCCGCTGTCATGTGGCCTGATATGGACTCTGGTGTCACCAAGACCCGATTGACTATTGACGATATTGAAGGCATCCGTGCTTTATATGGCCGTTAA

mRNA sequence

ATGGCTTCTAAATTATGTCTCCAACTCTTCCTACTTCTTGCCTTCATTACACCTCACTTGACCTCAACCCGCCCTCTTCACAACCACAAATCATCTCGACTTCTATTTCCCAAGCATCTTCAAGGAAGTCGTAAAGACGATAACATCGAAGGAATCCATAAGGTCAAGGCTTACCTTCAACGTTATGGTTACTTAAGCAATAAAAGCGAAAACAAATTGAATACAGACTCATTCGACGAGGACTTGGAATCCGCCATTAAATCATACCAAACGTTCTTCAAGCTTAATGTGAGTGGAGTCTTAGACAAGGAGACCCTACAACAAATGTCGAAGCCTCGATGTGGGGTGACAGACAATTTCATAGTATCACAAGGTAAGAATGTGGAAATGAATGGTTCTCATTATTCATTCTTCCCAAAGAAACGAACATGGCCAGTTGAGAAGAAGCATTTGACGTACGGATTTATCCACAATTACCCACCAGAGCACGTGGCTGCAGTGATGCGAGCTTTAGAGACATGGGCAGATAATACGAAATTCACCTTTGCCCAGGCTGCAAGGGTACAATCGGCAGACATTTTGTTAAGCTTTGAGAGAGGAGACCATGGAGATGGGCGTCCGTTTGATGGAGAAGGGGGAGTATTGGCACATGCATTTGGACCAATAGATGGGAGAATACATTTTGATGCGGATGAGCCATGGGAAAGAGGTCCTATTTTGGAACAGTTTGATGTGGAGTCGGTGGCATTGCATGAGTTGGGACATGCACTTGGGCTTGGCCATAGCAACATTGAATCCGCTGTCATGTGGCCTGATATGGACTCTGGTGTCACCAAGACCCGATTGACTATTGACGATATTGAAGGCATCCGTGCTTTATATGGCCGTTAA

Coding sequence (CDS)

ATGGCTTCTAAATTATGTCTCCAACTCTTCCTACTTCTTGCCTTCATTACACCTCACTTGACCTCAACCCGCCCTCTTCACAACCACAAATCATCTCGACTTCTATTTCCCAAGCATCTTCAAGGAAGTCGTAAAGACGATAACATCGAAGGAATCCATAAGGTCAAGGCTTACCTTCAACGTTATGGTTACTTAAGCAATAAAAGCGAAAACAAATTGAATACAGACTCATTCGACGAGGACTTGGAATCCGCCATTAAATCATACCAAACGTTCTTCAAGCTTAATGTGAGTGGAGTCTTAGACAAGGAGACCCTACAACAAATGTCGAAGCCTCGATGTGGGGTGACAGACAATTTCATAGTATCACAAGGTAAGAATGTGGAAATGAATGGTTCTCATTATTCATTCTTCCCAAAGAAACGAACATGGCCAGTTGAGAAGAAGCATTTGACGTACGGATTTATCCACAATTACCCACCAGAGCACGTGGCTGCAGTGATGCGAGCTTTAGAGACATGGGCAGATAATACGAAATTCACCTTTGCCCAGGCTGCAAGGGTACAATCGGCAGACATTTTGTTAAGCTTTGAGAGAGGAGACCATGGAGATGGGCGTCCGTTTGATGGAGAAGGGGGAGTATTGGCACATGCATTTGGACCAATAGATGGGAGAATACATTTTGATGCGGATGAGCCATGGGAAAGAGGTCCTATTTTGGAACAGTTTGATGTGGAGTCGGTGGCATTGCATGAGTTGGGACATGCACTTGGGCTTGGCCATAGCAACATTGAATCCGCTGTCATGTGGCCTGATATGGACTCTGGTGTCACCAAGACCCGATTGACTATTGACGATATTGAAGGCATCCGTGCTTTATATGGCCGTTAA

Protein sequence

MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEPWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYGR

Homology

[Close]

BLAST of Lsi02G015340 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 5.4e-55
Identity = 116/270 (42.96%), Postives = 151/270 (55.93%), Query Frame = 0


Query: 40  LQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSG 99
           L G    +NI G+ K+K Y +R+GY++        TD FD+ L+SAI +YQ  F L V+G
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGN---CTDDFDDVLQSAINTYQKNFNLKVTG 107

Query: 100 VLDKETLQQMSKPRCG---VTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFI 159
            LD  TL+Q+ KPRCG   + D      G  +      YSFFP K  WP  K+ LTY F 
Sbjct: 108 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFA 167

Query: 160 --HNYPPEHVAAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGV 219
             +N   E      RA   WA+ T   F ++  +  ADI++ F  G+HGDG PFDG  G 
Sbjct: 168 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 227

Query: 220 LAHAFGPIDGRIHFDADEPW--ERG-------PILEQFDVESVALHELGHALGLGHSNIE 279
           LAHA  P  G +H D DE W    G       P+    D+ESVA+HE+GH LGLGHS++E
Sbjct: 228 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 287

Query: 280 SAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
            A+M+P +  G  K  L  DDIEGI+ LYG
Sbjct: 288 DAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Lsi02G015340 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.9e-53
Identity = 126/319 (39.50%), Postives = 179/319 (56.11%), Query Frame = 0


Query: 7   LQLFLLL----AFITPHLTSTRP-LHNHKSSRLLFP--KHLQGSRKDDNIEGIHKVKAYL 66
           L LFL++    A+  P+ T+  P L N  ++R+ +    +  G     N++G++++K Y 
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRN--TTRVFWDAFSNFTGCHHGQNVDGLYRIKKYF 68

Query: 67  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCG---- 126
           QR+GY+         TD FD+ L++A++ YQT F LNV+G LD  T+Q +  PRCG    
Sbjct: 69  QRFGYIPETFSGNF-TDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDV 128

Query: 127 VTDNFIVSQG--KNVEMNGS--------HYSFFPKKRTWPVEKKHLTYGFIHNYP-PEHV 186
           V    ++  G  K  E+N S         Y+ FP +  WP  ++ LTY F    P  E V
Sbjct: 129 VNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEV 188

Query: 187 AAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPID 246
            +V  RA   W+D T   F  +    ++DI + F  GDHGDG PFDG  G LAHAF P  
Sbjct: 189 KSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPS 248

Query: 247 GRIHFDADEPWERGPILEQF-------DVESVALHELGHALGLGHSNIESAVMWPDMDSG 296
           G+ H DADE W     L+ F       D+ESVA+HE+GH LGLGHS++E ++M+P + +G
Sbjct: 249 GKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTG 308

[Close]

BLAST of Lsi02G015340 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 112/279 (40.14%), Postives = 158/279 (56.63%), Query Frame = 0


Query: 42  GSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVL 101
           G       +G++ +K Y Q +GY++  + +   TD FD+ L++A++ YQ  F+LNV+GVL
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 102 DKETLQQMSKPRCGVTD-------------NFIVS-QGKNVEMNG-SHYSFFPKKRTWPV 161
           D+ TL+ +  PRCG  D              F VS  G+    +   HYSFFP +  WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 162 EKKHLTYGF-IHNYPPEHVAAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGD 221
            ++ LTY F   N   E V +V  RA   W + T  TF +  R  ++DI + F  G+HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 222 GRPFDGEGGVLAHAFGPIDGRIHFDADEPW----ERG----PILEQFDVESVALHELGHA 281
           G PFDG    LAHAF P  G  H D +E W    E G     + E  D+ESVA+HE+GH 
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 282 LGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
           LGLGHS++E ++M+P + +G  K  LT DD+EG++ LYG
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Lsi02G015340 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 9.9e-49
Identity = 106/257 (41.25%), Postives = 138/257 (53.70%), Query Frame = 0


Query: 48  NIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQ 107
           N +G+  VK Y    GY+ N        D+FD+ L SAIK+YQ  + LNV+G  D  TL+
Sbjct: 50  NYKGLSNVKNYFHHLGYIPNAPH---FDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLK 109

Query: 108 QMSKPRCGVTDNFIVSQGKNVEMNG-SHYSFFPKKRTWPVEKKHLTYGFI--HNYPPEHV 167
           Q+  PRCGV D  I++  K       S Y+FF     W      LTY F           
Sbjct: 110 QIMTPRCGVPD-IIINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFK 169

Query: 168 AAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDG 227
           +A+ RA   W       F +    ++A+I + F   +HGD  PFDG GG+L HAF P DG
Sbjct: 170 SAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDG 229

Query: 228 RIHFDADEPW------ERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVT 287
           R HFDADE W       + P+   FD+ESVA+HE+GH LGLGHS+   A+M+P +     
Sbjct: 230 RCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTR 289

Query: 288 KTRLTIDDIEGIRALYG 296
           K  L  DDI+GIR LYG
Sbjct: 290 KVNLAQDDIDGIRKLYG 302

[Close]

BLAST of Lsi02G015340 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-45
Identity = 102/259 (39.38%), Postives = 145/259 (55.98%), Query Frame = 0


Query: 48  NIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQ 107
           ++ G+ ++K YL R+GY+++ SE  + +D FD  LESAI  YQ    L ++G LD  T+ 
Sbjct: 66  HVSGVSELKRYLHRFGYVNDGSE--IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVT 125

Query: 108 QMSKPRCGVTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAA- 167
            MS PRCGV+D  +      +    +HY++F  K  W   +  LTY     +  +++ + 
Sbjct: 126 LMSLPRCGVSDTHMTINNDFLHTT-AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSE 185

Query: 168 -----VMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGP 227
                  RA   W+     +F +     +AD+ + F  GDHGDG PFDG  G LAHAF P
Sbjct: 186 DVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAP 245

Query: 228 IDGRIHFDADEPW-----ERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSG 287
            +GR+H DA E W      +G      D+ESVA HE+GH LGLGHS+ ESAVM+P +   
Sbjct: 246 ENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPR 305

Query: 288 VTKTRLTIDDIEGIRALYG 296
             K  LT+DD+ G+  LYG
Sbjct: 306 TKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Lsi02G015340 vs. ExPASy TrEMBL
Match: A0A5D3BUL7 (Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00140 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 9.1e-130
Identity = 229/303 (75.58%), Postives = 254/303 (83.83%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQ 60
           MASKLCLQLFLL AFITPHLTS R L NHK SRLLFP+HL GSRK+DN EGIHKVKAYLQ
Sbjct: 1   MASKLCLQLFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYLQ 60

Query: 61  RYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNF 120
           RYGYLS++ EN L+TD+FD+DLESAIKSYQ  FKLNVSGVLD+ETLQQM++PRCGVTD+F
Sbjct: 61  RYGYLSDEKENNLSTDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDF 120

Query: 121 IV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALET 180
           I+       +   N+E+ GSHY F+P K  WPVEK+HLTYGFIHNYPPEH AAV+RA +T
Sbjct: 121 ILVAQQDHENNNTNIEIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDT 180

Query: 181 WADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEP 240
           W  NTKFTF+ AARVQ+ADILLSFERGDHGDG+PFDG GG L HAFG IDGR+HFD DE 
Sbjct: 181 WEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQ 240

Query: 241 WERGPI-LEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           WE G    E FDVESVALHE+GHALGLGHS   SAVMW +MDSGVTKT LTIDDIEGI A
Sbjct: 241 WETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDDIEGIHA 300

[Close]

BLAST of Lsi02G015340 vs. ExPASy TrEMBL
Match: A0A1S3C8Z2 (metalloendoproteinase 3-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498174 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.9e-128
Identity = 226/303 (74.59%), Postives = 252/303 (83.17%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQ 60
           M SKLCLQLFLL AFITPHLTS R L NHK SRLLFP+HL GSRK+DN EGIHKVKAYLQ
Sbjct: 1   MGSKLCLQLFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYLQ 60

Query: 61  RYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNF 120
           RYGYLS++ EN L+ D+FD+DLESAIKSYQ  FKLNVSGVLD+ETLQQM++PRCGVTD+F
Sbjct: 61  RYGYLSDEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDF 120

Query: 121 IV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALET 180
           I+       +   N+++ GSHY F+P K  WPVEK+HLTYGFIHNYPPEH AAV+RA +T
Sbjct: 121 ILVAQQDHENNNTNIQIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDT 180

Query: 181 WADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEP 240
           W  NTKFTF+ AARVQ+ADILLSFERGDHGDG+PFDG GG L HAFG IDGR+HFD DE 
Sbjct: 181 WEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQ 240

Query: 241 WERGPI-LEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           WE G    E FDVESVALHE+GHALGLGHS   SAVMW +MDSGVTKT LTIDDIEGI A
Sbjct: 241 WETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDDIEGIHA 300

[Close]

BLAST of Lsi02G015340 vs. ExPASy TrEMBL
Match: A0A0A0KS35 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 8.5e-120
Identity = 217/303 (71.62%), Postives = 250/303 (82.51%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MA+KLCLQLFLLLAF+TPHLTS RPLHNHKSSRLLFP+HL GS K+D+I EGIHKVKAYL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++EN L+TD+FD+DLESAIKSYQ F  L VSGVLD+ETLQQMS+PRCGV DN
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120

Query: 121 F---IVSQGKN----VEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F       G+N    V++ GSH+ FFP K  WP  K HLTYGF+HNYP +H AAV+RA +
Sbjct: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
            WA N+KFTF+ A R+Q+ADILLSFERGDHGDG+PFDGEGG+L HAFGPIDGR+HFDADE
Sbjct: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240

Query: 241 PW-ERGPIL-EQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGI 294
            W E G +  E FD ESVALHE+GHALGLGHS   SAVMW +M++GV KT LTIDDIEG+
Sbjct: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300

[Close]

BLAST of Lsi02G015340 vs. ExPASy TrEMBL
Match: A0A5A7T1Y6 (Metalloendoproteinase 5-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00290 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.1e-119
Identity = 216/303 (71.29%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQLFLLLAF+TPHLTS R LHNHK SRLLFP+HL GS K+DNI EGIHKVKAYL
Sbjct: 38  MASKLCLQLFLLLAFVTPHLTSARHLHNHKPSRLLFPEHLIGSSKNDNIVEGIHKVKAYL 97

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++ N L+TD FD DLESAIKSYQ F KL VSGVLD+ETLQQMSKPRCGV D 
Sbjct: 98  QRYGYFSNENANTLSTDVFDNDLESAIKSYQKFSKLKVSGVLDRETLQQMSKPRCGVRDT 157

Query: 121 FI-------VSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F+        ++  N+E+ GSHY+F+    TWP EKKHL+YGFIHNYPP+ V  ++RA E
Sbjct: 158 FVSVAQQEHENKNTNIEIGGSHYTFYFNNVTWPAEKKHLSYGFIHNYPPQFVDPLVRAFE 217

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  A RVQ+ADIL+SFERGDHGD + FDGEGG+LAH+ G +DGR+HFDADE
Sbjct: 218 TWEDNTKFSFFLAPRVQTADILVSFERGDHGDYQAFDGEGGILAHSLGAVDGRVHFDADE 277

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           PWE GPI ++ D+ESVALHELGH LGLGHS   SAVM+  MD GV KT LTIDDIEGI A
Sbjct: 278 PWESGPIEDRVDLESVALHELGHTLGLGHSREPSAVMYAFMDPGVYKTHLTIDDIEGICA 337

[Close]

BLAST of Lsi02G015340 vs. ExPASy TrEMBL
Match: A0A1S3CA77 (metalloendoproteinase 5-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498176 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.1e-119
Identity = 216/303 (71.29%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQLFLLLAF+TPHLTS R LHNHK SRLLFP+HL GS K+DNI EGIHKVKAYL
Sbjct: 1   MASKLCLQLFLLLAFVTPHLTSARHLHNHKPSRLLFPEHLIGSSKNDNIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++ N L+TD FD DLESAIKSYQ F KL VSGVLD+ETLQQMSKPRCGV D 
Sbjct: 61  QRYGYFSNENANTLSTDVFDNDLESAIKSYQKFSKLKVSGVLDRETLQQMSKPRCGVRDT 120

Query: 121 FI-------VSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F+        ++  N+E+ GSHY+F+    TWP EKKHL+YGFIHNYPP+ V  ++RA E
Sbjct: 121 FVSVAQQEHENKNTNIEIGGSHYTFYFNNVTWPAEKKHLSYGFIHNYPPQFVDPLVRAFE 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  A RVQ+ADIL+SFERGDHGD + FDGEGG+LAH+ G +DGR+HFDADE
Sbjct: 181 TWEDNTKFSFFLAPRVQTADILVSFERGDHGDYQAFDGEGGILAHSLGAVDGRVHFDADE 240

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           PWE GPI ++ D+ESVALHELGH LGLGHS   SAVM+  MD GV KT LTIDDIEGI A
Sbjct: 241 PWESGPIEDRVDLESVALHELGHTLGLGHSREPSAVMYAFMDPGVYKTHLTIDDIEGICA 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: KAA0037452.1 (metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa] >TYK01926.1 metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 473.0 bits (1216), Expect = 1.9e-129
Identity = 229/303 (75.58%), Postives = 254/303 (83.83%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQ 60
           MASKLCLQLFLL AFITPHLTS R L NHK SRLLFP+HL GSRK+DN EGIHKVKAYLQ
Sbjct: 1   MASKLCLQLFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYLQ 60

Query: 61  RYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNF 120
           RYGYLS++ EN L+TD+FD+DLESAIKSYQ  FKLNVSGVLD+ETLQQM++PRCGVTD+F
Sbjct: 61  RYGYLSDEKENNLSTDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDF 120

Query: 121 IV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALET 180
           I+       +   N+E+ GSHY F+P K  WPVEK+HLTYGFIHNYPPEH AAV+RA +T
Sbjct: 121 ILVAQQDHENNNTNIEIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDT 180

Query: 181 WADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEP 240
           W  NTKFTF+ AARVQ+ADILLSFERGDHGDG+PFDG GG L HAFG IDGR+HFD DE 
Sbjct: 181 WEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQ 240

Query: 241 WERGPI-LEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           WE G    E FDVESVALHE+GHALGLGHS   SAVMW +MDSGVTKT LTIDDIEGI A
Sbjct: 241 WETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDDIEGIHA 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: XP_008458911.1 (PREDICTED: metalloendoproteinase 3-MMP-like [Cucumis melo])

HSP 1 Score: 468.0 bits (1203), Expect = 6.0e-128
Identity = 226/303 (74.59%), Postives = 252/303 (83.17%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQ 60
           M SKLCLQLFLL AFITPHLTS R L NHK SRLLFP+HL GSRK+DN EGIHKVKAYLQ
Sbjct: 1   MGSKLCLQLFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYLQ 60

Query: 61  RYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNF 120
           RYGYLS++ EN L+ D+FD+DLESAIKSYQ  FKLNVSGVLD+ETLQQM++PRCGVTD+F
Sbjct: 61  RYGYLSDEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDF 120

Query: 121 IV-------SQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALET 180
           I+       +   N+++ GSHY F+P K  WPVEK+HLTYGFIHNYPPEH AAV+RA +T
Sbjct: 121 ILVAQQDHENNNTNIQIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDT 180

Query: 181 WADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEP 240
           W  NTKFTF+ AARVQ+ADILLSFERGDHGDG+PFDG GG L HAFG IDGR+HFD DE 
Sbjct: 181 WEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQ 240

Query: 241 WERGPI-LEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           WE G    E FDVESVALHE+GHALGLGHS   SAVMW +MDSGVTKT LTIDDIEGI A
Sbjct: 241 WETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDDIEGIHA 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: XP_004142466.2 (metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52408.1 hypothetical protein Csa_008745 [Cucumis sativus])

HSP 1 Score: 439.9 bits (1130), Expect = 1.8e-119
Identity = 217/303 (71.62%), Postives = 250/303 (82.51%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MA+KLCLQLFLLLAF+TPHLTS RPLHNHKSSRLLFP+HL GS K+D+I EGIHKVKAYL
Sbjct: 1   MAAKLCLQLFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++EN L+TD+FD+DLESAIKSYQ F  L VSGVLD+ETLQQMS+PRCGV DN
Sbjct: 61  QRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADN 120

Query: 121 F---IVSQGKN----VEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F       G+N    V++ GSH+ FFP K  WP  K HLTYGF+HNYP +H AAV+RA +
Sbjct: 121 FQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFD 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
            WA N+KFTF+ A R+Q+ADILLSFERGDHGDG+PFDGEGG+L HAFGPIDGR+HFDADE
Sbjct: 181 KWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADE 240

Query: 241 PW-ERGPIL-EQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGI 294
            W E G +  E FD ESVALHE+GHALGLGHS   SAVMW +M++GV KT LTIDDIEG+
Sbjct: 241 QWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGV 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: XP_008458913.1 (PREDICTED: metalloendoproteinase 5-MMP-like [Cucumis melo])

HSP 1 Score: 439.5 bits (1129), Expect = 2.3e-119
Identity = 216/303 (71.29%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQLFLLLAF+TPHLTS R LHNHK SRLLFP+HL GS K+DNI EGIHKVKAYL
Sbjct: 1   MASKLCLQLFLLLAFVTPHLTSARHLHNHKPSRLLFPEHLIGSSKNDNIVEGIHKVKAYL 60

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++ N L+TD FD DLESAIKSYQ F KL VSGVLD+ETLQQMSKPRCGV D 
Sbjct: 61  QRYGYFSNENANTLSTDVFDNDLESAIKSYQKFSKLKVSGVLDRETLQQMSKPRCGVRDT 120

Query: 121 FI-------VSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F+        ++  N+E+ GSHY+F+    TWP EKKHL+YGFIHNYPP+ V  ++RA E
Sbjct: 121 FVSVAQQEHENKNTNIEIGGSHYTFYFNNVTWPAEKKHLSYGFIHNYPPQFVDPLVRAFE 180

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  A RVQ+ADIL+SFERGDHGD + FDGEGG+LAH+ G +DGR+HFDADE
Sbjct: 181 TWEDNTKFSFFLAPRVQTADILVSFERGDHGDYQAFDGEGGILAHSLGAVDGRVHFDADE 240

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           PWE GPI ++ D+ESVALHELGH LGLGHS   SAVM+  MD GV KT LTIDDIEGI A
Sbjct: 241 PWESGPIEDRVDLESVALHELGHTLGLGHSREPSAVMYAFMDPGVYKTHLTIDDIEGICA 300

[Close]

BLAST of Lsi02G015340 vs. NCBI nr
Match: KAA0037462.1 (metalloendoproteinase 5-MMP-like [Cucumis melo var. makuwa] >TYK01938.1 metalloendoproteinase 5-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 439.5 bits (1129), Expect = 2.3e-119
Identity = 216/303 (71.29%), Postives = 246/303 (81.19%), Query Frame = 0


Query: 1   MASKLCLQLFLLLAFITPHLTSTRPLHNHKSSRLLFPKHLQGSRKDDNI-EGIHKVKAYL 60
           MASKLCLQLFLLLAF+TPHLTS R LHNHK SRLLFP+HL GS K+DNI EGIHKVKAYL
Sbjct: 38  MASKLCLQLFLLLAFVTPHLTSARHLHNHKPSRLLFPEHLIGSSKNDNIVEGIHKVKAYL 97

Query: 61  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDN 120
           QRYGY SN++ N L+TD FD DLESAIKSYQ F KL VSGVLD+ETLQQMSKPRCGV D 
Sbjct: 98  QRYGYFSNENANTLSTDVFDNDLESAIKSYQKFSKLKVSGVLDRETLQQMSKPRCGVRDT 157

Query: 121 FI-------VSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAAVMRALE 180
           F+        ++  N+E+ GSHY+F+    TWP EKKHL+YGFIHNYPP+ V  ++RA E
Sbjct: 158 FVSVAQQEHENKNTNIEIGGSHYTFYFNNVTWPAEKKHLSYGFIHNYPPQFVDPLVRAFE 217

Query: 181 TWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADE 240
           TW DNTKF+F  A RVQ+ADIL+SFERGDHGD + FDGEGG+LAH+ G +DGR+HFDADE
Sbjct: 218 TWEDNTKFSFFLAPRVQTADILVSFERGDHGDYQAFDGEGGILAHSLGAVDGRVHFDADE 277

Query: 241 PWERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRA 296
           PWE GPI ++ D+ESVALHELGH LGLGHS   SAVM+  MD GV KT LTIDDIEGI A
Sbjct: 278 PWESGPIEDRVDLESVALHELGHTLGLGHSREPSAVMYAFMDPGVYKTHLTIDDIEGICA 337

[Close]

BLAST of Lsi02G015340 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 216.1 bits (549), Expect = 3.8e-56
Identity = 116/270 (42.96%), Postives = 151/270 (55.93%), Query Frame = 0


Query: 40  LQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSG 99
           L G    +NI G+ K+K Y +R+GY++        TD FD+ L+SAI +YQ  F L V+G
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGN---CTDDFDDVLQSAINTYQKNFNLKVTG 107

Query: 100 VLDKETLQQMSKPRCG---VTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFI 159
            LD  TL+Q+ KPRCG   + D      G  +      YSFFP K  WP  K+ LTY F 
Sbjct: 108 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFA 167

Query: 160 --HNYPPEHVAAVMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGV 219
             +N   E      RA   WA+ T   F ++  +  ADI++ F  G+HGDG PFDG  G 
Sbjct: 168 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 227

Query: 220 LAHAFGPIDGRIHFDADEPW--ERG-------PILEQFDVESVALHELGHALGLGHSNIE 279
           LAHA  P  G +H D DE W    G       P+    D+ESVA+HE+GH LGLGHS++E
Sbjct: 228 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 287

Query: 280 SAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
            A+M+P +  G  K  L  DDIEGI+ LYG
Sbjct: 288 DAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Lsi02G015340 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 209.9 bits (533), Expect = 2.8e-54
Identity = 126/319 (39.50%), Postives = 179/319 (56.11%), Query Frame = 0


Query: 7   LQLFLLL----AFITPHLTSTRP-LHNHKSSRLLFP--KHLQGSRKDDNIEGIHKVKAYL 66
           L LFL++    A+  P+ T+  P L N  ++R+ +    +  G     N++G++++K Y 
Sbjct: 9   LSLFLIVSPASAWFFPNSTAVPPSLRN--TTRVFWDAFSNFTGCHHGQNVDGLYRIKKYF 68

Query: 67  QRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQQMSKPRCG---- 126
           QR+GY+         TD FD+ L++A++ YQT F LNV+G LD  T+Q +  PRCG    
Sbjct: 69  QRFGYIPETFSGNF-TDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDV 128

Query: 127 VTDNFIVSQG--KNVEMNGS--------HYSFFPKKRTWPVEKKHLTYGFIHNYP-PEHV 186
           V    ++  G  K  E+N S         Y+ FP +  WP  ++ LTY F    P  E V
Sbjct: 129 VNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEV 188

Query: 187 AAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGPID 246
            +V  RA   W+D T   F  +    ++DI + F  GDHGDG PFDG  G LAHAF P  
Sbjct: 189 KSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPS 248

Query: 247 GRIHFDADEPWERGPILEQF-------DVESVALHELGHALGLGHSNIESAVMWPDMDSG 296
           G+ H DADE W     L+ F       D+ESVA+HE+GH LGLGHS++E ++M+P + +G
Sbjct: 249 GKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTG 308

[Close]

BLAST of Lsi02G015340 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 201.8 bits (512), Expect = 7.5e-52
Identity = 112/279 (40.14%), Postives = 158/279 (56.63%), Query Frame = 0


Query: 42  GSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVL 101
           G       +G++ +K Y Q +GY++  + +   TD FD+ L++A++ YQ  F+LNV+GVL
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 102 DKETLQQMSKPRCGVTD-------------NFIVS-QGKNVEMNG-SHYSFFPKKRTWPV 161
           D+ TL+ +  PRCG  D              F VS  G+    +   HYSFFP +  WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 162 EKKHLTYGF-IHNYPPEHVAAVM-RALETWADNTKFTFAQAARVQSADILLSFERGDHGD 221
            ++ LTY F   N   E V +V  RA   W + T  TF +  R  ++DI + F  G+HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 222 GRPFDGEGGVLAHAFGPIDGRIHFDADEPW----ERG----PILEQFDVESVALHELGHA 281
           G PFDG    LAHAF P  G  H D +E W    E G     + E  D+ESVA+HE+GH 
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 282 LGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
           LGLGHS++E ++M+P + +G  K  LT DD+EG++ LYG
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Lsi02G015340 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 184.9 bits (468), Expect = 9.5e-47
Identity = 102/259 (39.38%), Postives = 145/259 (55.98%), Query Frame = 0


Query: 48  NIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESAIKSYQTFFKLNVSGVLDKETLQ 107
           ++ G+ ++K YL R+GY+++ SE  + +D FD  LESAI  YQ    L ++G LD  T+ 
Sbjct: 66  HVSGVSELKRYLHRFGYVNDGSE--IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVT 125

Query: 108 QMSKPRCGVTDNFIVSQGKNVEMNGSHYSFFPKKRTWPVEKKHLTYGFIHNYPPEHVAA- 167
            MS PRCGV+D  +      +    +HY++F  K  W   +  LTY     +  +++ + 
Sbjct: 126 LMSLPRCGVSDTHMTINNDFLHTT-AHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSE 185

Query: 168 -----VMRALETWADNTKFTFAQAARVQSADILLSFERGDHGDGRPFDGEGGVLAHAFGP 227
                  RA   W+     +F +     +AD+ + F  GDHGDG PFDG  G LAHAF P
Sbjct: 186 DVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAP 245

Query: 228 IDGRIHFDADEPW-----ERGPILEQFDVESVALHELGHALGLGHSNIESAVMWPDMDSG 287
            +GR+H DA E W      +G      D+ESVA HE+GH LGLGHS+ ESAVM+P +   
Sbjct: 246 ENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPR 305

Query: 288 VTKTRLTIDDIEGIRALYG 296
             K  LT+DD+ G+  LYG
Sbjct: 306 TKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Lsi02G015340 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 163.3 bits (412), Expect = 3.0e-40
Identity = 101/283 (35.69%), Postives = 139/283 (49.12%), Query Frame = 0


Query: 26  LHNHKSSRLLFPKHLQGSRKDDNIEGIHKVKAYLQRYGYLSNKSENKLNTDSFDEDLESA 85
           LHN +      P     +   D    I ++K +LQ+YGYL        N +S D   E A
Sbjct: 26  LHNQQIIEARNPSQFTTNPSPD--VSIPEIKRHLQQYGYLPQ------NKESDDVSFEQA 85

Query: 86  IKSYQTFFKLNVSGVLDKETLQQMSKPRCGVTDNFIVSQGKNVEMNGSHYSFFPKKRTWP 145
           +  YQ    L ++G  D +TL Q+  PRCG  D+  V         G  Y +FP +  W 
Sbjct: 86  LVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDD--VEPKTAPFHTGKKYVYFPGRPRWT 145

Query: 146 VE-KKHLTYGFIHNYPPEHVA------AVMRALETWADNTKFTFAQAARVQSADILLSFE 205
            +    LTY F       ++A         RA   WA     +F +      ADI + F 
Sbjct: 146 RDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFF 205

Query: 206 RGDHGDGRPFDGEGGVLAHAFGPIDGRIHFDADEPW------ERGPILEQFDVESVALHE 265
            GDHGDG PFDG  GVLAH F P +GR+H D  E W      E+  +    D+ESVA+HE
Sbjct: 206 NGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSV--AVDLESVAVHE 265

Query: 266 LGHALGLGHSNIESAVMWPDMDSGVTKTRLTIDDIEGIRALYG 296
           +GH LGLGHS+++ A M+P +     K  L +DD+ G+++LYG
Sbjct: 266 IGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYG 296

The following BLAST results are available for this feature:

BLAST of Lsi02G015340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZUJ5	5.4e-55	42.96	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O04529	3.9e-53	39.50	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q5XF51	1.1e-50	40.14	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
P29136	9.9e-49	41.25	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O23507	1.3e-45	39.38	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of Lsi02G015340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BUL7	9.1e-130	75.58	Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C8Z2	2.9e-128	74.59	metalloendoproteinase 3-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498174 PE=3 SV...	[more]
A0A0A0KS35	8.5e-120	71.62	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632070 PE=3 S...	[more]
A0A5A7T1Y6	1.1e-119	71.29	Metalloendoproteinase 5-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CA77	1.1e-119	71.29	metalloendoproteinase 5-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498176 PE=3 SV...	[more]

BLAST of Lsi02G015340 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0037452.1	1.9e-129	75.58	metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa] >TYK01926.1 metalloe...	[more]
XP_008458911.1	6.0e-128	74.59	PREDICTED: metalloendoproteinase 3-MMP-like [Cucumis melo]	[more]
XP_004142466.2	1.8e-119	71.62	metalloendoproteinase 2-MMP [Cucumis sativus] >KGN52408.1 hypothetical protein C...	[more]
XP_008458913.1	2.3e-119	71.29	PREDICTED: metalloendoproteinase 5-MMP-like [Cucumis melo]	[more]
KAA0037462.1	2.3e-119	71.29	metalloendoproteinase 5-MMP-like [Cucumis melo var. makuwa] >TYK01938.1 metalloe...	[more]

BLAST of Lsi02G015340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59970.1	3.8e-56	42.96	Matrixin family protein	[more]
AT1G70170.1	2.8e-54	39.50	matrix metalloproteinase	[more]
AT1G24140.1	7.5e-52	40.14	Matrixin family protein	[more]
AT4G16640.1	9.5e-47	39.38	Matrixin family protein	[more]
AT2G45040.1	3.0e-40	35.69	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 109..122 score: 51.57 coord: 191..219 score: 62.07 coord: 167..182 score: 33.23 coord: 282..295 score: 59.58 coord: 248..273 score: 57.65
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 140..296 e-value: 6.6E-29 score: 112.0
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 50..109 e-value: 1.4E-14 score: 54.0
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 40..296 e-value: 7.5E-81 score: 273.2
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 147..295 e-value: 2.3E-42 score: 144.5
None	No IPR available	PIRSR	PIRSR001191-1	PIRSR001191-1	coord: 53..295 e-value: 1.1E-66 score: 223.3
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 23..295
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 23..295
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 137..295
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 147..295 e-value: 2.73041E-61 score: 189.723
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 46..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G015340.1	Lsi02G015340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity