Lsi02G015220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G015220
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description2S albumin
Locationchr02: 21127668 .. 21128102 (+)
RNA-Seq ExpressionLsi02G015220
SyntenyLsi02G015220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGATCGGTAGTGATCAGAACTATCATTGCTCTGTTGGCAGCGGCCTTGGTGGTTGCAGATGCCCACCGCACCATCATCAGGACGGTGGAAGTGGAGGAAGAGAACCAAGGGCGGGGGCAGCAGCAGAGGTGCCGGCAAATGAGGGCCCAGGAGGAGATAGGGAGCTGCCAAGAGTACCTGAGGCAGCAGAGCAGATATGTTTTGGAGATGCGTGGAATTGAAAACCAGAGGAGGAGAGGAGGGGATTTGTTCAATGAGTGTTGCCGTGAACTGAGGAATGTGGATGAGGAATGTAGGTGTGAACTGTTGGAAGAGATTGCTGGGATGGAGCGGAGGAAAGGAGGAGGGCCAGAAGAGAGGCAATTGTTTCAGAGAGCTAGAAACTTGCCATCCATGTGTGGAATCCGCCCACAGCAATGCTACTTCTAA

mRNA sequence

ATGGGAAGATCGGTAGTGATCAGAACTATCATTGCTCTGTTGGCAGCGGCCTTGGTGGTTGCAGATGCCCACCGCACCATCATCAGGACGGTGGAAGTGGAGGAAGAGAACCAAGGGCGGGGGCAGCAGCAGAGGTGCCGGCAAATGAGGGCCCAGGAGGAGATAGGGAGCTGCCAAGAGTACCTGAGGCAGCAGAGCAGATATGTTTTGGAGATGCGTGGAATTGAAAACCAGAGGAGGAGAGGAGGGGATTTGTTCAATGAGTGTTGCCGTGAACTGAGGAATGTGGATGAGGAATGTAGGTGTGAACTGTTGGAAGAGATTGCTGGGATGGAGCGGAGGAAAGGAGGAGGGCCAGAAGAGAGGCAATTGTTTCAGAGAGCTAGAAACTTGCCATCCATGTGTGGAATCCGCCCACAGCAATGCTACTTCTAA

Coding sequence (CDS)

ATGGGAAGATCGGTAGTGATCAGAACTATCATTGCTCTGTTGGCAGCGGCCTTGGTGGTTGCAGATGCCCACCGCACCATCATCAGGACGGTGGAAGTGGAGGAAGAGAACCAAGGGCGGGGGCAGCAGCAGAGGTGCCGGCAAATGAGGGCCCAGGAGGAGATAGGGAGCTGCCAAGAGTACCTGAGGCAGCAGAGCAGATATGTTTTGGAGATGCGTGGAATTGAAAACCAGAGGAGGAGAGGAGGGGATTTGTTCAATGAGTGTTGCCGTGAACTGAGGAATGTGGATGAGGAATGTAGGTGTGAACTGTTGGAAGAGATTGCTGGGATGGAGCGGAGGAAAGGAGGAGGGCCAGAAGAGAGGCAATTGTTTCAGAGAGCTAGAAACTTGCCATCCATGTGTGGAATCCGCCCACAGCAATGCTACTTCTAA

Protein sequence

MGRSVVIRTIIALLAAALVVADAHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQQSRYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQLFQRARNLPSMCGIRPQQCYF
Homology
BLAST of Lsi02G015220 vs. ExPASy Swiss-Prot
Match: Q39649 (2S albumin OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.7e-41
Identity = 93/140 (66.43%), Postives = 113/140 (80.71%), Query Frame = 0

Query: 7   IRTIIALLAAALVVAD--AHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQ 66
           + +IIAL A AL+VAD  A+RT I TVEVEE  QGR  ++RCRQM A+EE+ SC++YLRQ
Sbjct: 4   LTSIIALFAVALLVADAYAYRTTITTVEVEENRQGR--EERCRQMSAREELRSCEQYLRQ 63

Query: 67  QSRYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQL 126
           QSR VL+MRGIEN  RR G  F+ECCREL+NVDEECRC++LEEIA  E+R+  G E RQ+
Sbjct: 64  QSRDVLQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQARGQEGRQM 123

Query: 127 FQRARNLPSMCGIRPQQCYF 145
            Q+ARNLPSMCGIRPQ+C F
Sbjct: 124 LQKARNLPSMCGIRPQRCDF 141

BLAST of Lsi02G015220 vs. ExPASy Swiss-Prot
Match: P93198 (2S albumin seed storage protein (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.3e-13
Identity = 53/137 (38.69%), Postives = 84/137 (61.31%), Query Frame = 0

Query: 12  ALLAAALVVAD--AHRTIIRTVEVEEE-NQGRGQQQRCR-QMRAQEEIGSCQEYLRQQSR 71
           ALL A L VA+  A RT I T+E++E+ +  R + + CR Q++ Q+ +  CQ YLRQQSR
Sbjct: 2   ALLVALLFVANAAAFRTTITTMEIDEDIDNPRRRGEGCREQIQRQQNLNHCQYYLRQQSR 61

Query: 72  YVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGG--GPEERQLF 131
                 G +   +R    F +CC++L  +DE+C+CE L ++   ++++ G  G E  ++ 
Sbjct: 62  ----SGGYDEDNQR--QHFRQCCQQLSQMDEQCQCEGLRQVVRRQQQQQGLRGEEMEEMV 121

Query: 132 QRARNLPSMCGIRPQQC 143
           Q AR+LP+ CGI  Q+C
Sbjct: 122 QSARDLPNECGISSQRC 132

BLAST of Lsi02G015220 vs. ExPASy Swiss-Prot
Match: P0C8Y8 (2S sulfur-rich seed storage protein 2 OS=Bertholletia excelsa OX=3645 GN=BE2S2 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-10
Identity = 55/147 (37.41%), Postives = 81/147 (55.10%), Query Frame = 0

Query: 4   SVVIRTIIALLAAALVVADAHRTIIRTV---EVEEENQGRGQQQRCRQMRAQEEIGSCQE 63
           SVV   ++ALL   L  A A RT + T    E EE  +GR +QQ   QM  Q+++  C+ 
Sbjct: 5   SVVAAALLALL--VLGQATAFRTTVTTTLEEEQEENPRGRSEQQCREQMERQQQLNHCRM 64

Query: 64  YLRQQSRYVLEMRGIENQR--RRGGD-LFNECCRELRNVDEECRCELLEEIAGMERRKG- 123
           YLRQQ    +E    +N R  RRG +   +ECC +L  +DE CRCE L  +   +R +  
Sbjct: 65  YLRQQ----MEESPYQNPRPLRRGEEPHLDECCEQLERMDEMCRCEGLRMMLRRQREEME 124

Query: 124 -GGPEERQLFQRARNLPSMCGIRPQQC 143
             G + +++ ++A NL S C + PQ+C
Sbjct: 125 LQGEQMQRIMRKAENLLSRCNLSPQRC 145

BLAST of Lsi02G015220 vs. ExPASy Swiss-Prot
Match: P04403 (2S sulfur-rich seed storage protein 1 OS=Bertholletia excelsa OX=3645 GN=BE2S1 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 2.6e-10
Identity = 51/143 (35.66%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 11  IALLAAALVV------ADAHRTIIRTVEVEEENQGRGQQQRCR-QMRAQEEIGSCQEYLR 70
           I++ AAAL+V      A A R  + T  VEEEN     Q+ CR QM+ Q+ +  C+ Y+R
Sbjct: 4   ISVAAAALLVLMALGHATAFRATVTTTVVEEEN-----QEECREQMQRQQMLSHCRMYMR 63

Query: 71  QQSRYVLEMRGIENQRRRGGD-LFNECCRELRNVDEECRCELLEEI---AGMERRKGGGP 130
           QQ    +E    +   RRG +   +ECC +L  +DE CRCE L  +      E  +  G 
Sbjct: 64  QQ----MEESPYQTMPRRGMEPHMSECCEQLEGMDESCRCEGLRMMMMRMQQEEMQPRGE 123

Query: 131 EERQLFQRARNLPSMCGIRPQQC 143
           + R++ + A N+PS C + P +C
Sbjct: 124 QMRRMMRLAENIPSRCNLSPMRC 137

BLAST of Lsi02G015220 vs. ExPASy Swiss-Prot
Match: P15461 (2S seed storage protein OS=Helianthus annuus OX=4232 GN=HAG5 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.7e-09
Identity = 42/122 (34.43%), Postives = 63/122 (51.64%), Query Frame = 0

Query: 39  GRGQQQRCRQMRAQEEIGSCQEYLRQQ-------SRYVLEMRGIENQRRRGGDLFNECCR 98
           G G QQ CR+   Q  +G CQ ++ QQ       +R   +  G + Q++RG  L  +CC 
Sbjct: 172 GTGSQQ-CRETEIQRPVGECQRFVEQQMQQSPRSTRPYQQRPGQQQQQQRG--LQQQCCN 231

Query: 99  ELRNVDEECRCELLEEIA---------GMERRKG--GGPEERQLFQRARNLPSMCGIRPQ 143
           EL+NV  EC CE ++E+A           ++R+G  GG E     +  +NLP+ C +  Q
Sbjct: 232 ELQNVKRECHCEAIQEVARRVMRQPQQQQQQRRGQFGGQEMETARRVIQNLPNQCDLEVQ 290

BLAST of Lsi02G015220 vs. ExPASy TrEMBL
Match: A0A0A0L7Q2 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G080340 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.0e-45
Identity = 99/138 (71.74%), Postives = 116/138 (84.06%), Query Frame = 0

Query: 7   IRTIIALLAAALVVADAHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQQS 66
           + +IIALLA ALV+ADAHRT I TVEV+EEN GR   +RCRQMRA+EEIGSC +YL QQS
Sbjct: 4   LTSIIALLALALVIADAHRTTITTVEVDEENPGR--MERCRQMRAREEIGSCGQYLSQQS 63

Query: 67  RYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQLFQ 126
           RYVL+MRGI+NQRRRGG LF+ECC EL NV+EECRCELL+EIA  E+R+  G E  Q+ Q
Sbjct: 64  RYVLQMRGIDNQRRRGGQLFDECCSELTNVEEECRCELLQEIAMEEQRRARGQERTQMLQ 123

Query: 127 RARNLPSMCGIRPQQCYF 145
            A+NLPSMCG+RPQQCYF
Sbjct: 124 TAKNLPSMCGMRPQQCYF 139

BLAST of Lsi02G015220 vs. ExPASy TrEMBL
Match: A0A5A7SLZ0 (2S albumin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1112G00590 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.4e-45
Identity = 102/138 (73.91%), Postives = 115/138 (83.33%), Query Frame = 0

Query: 7   IRTIIALLAAALVVADAHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQQS 66
           + +IIALLA ALV+ADAHRT I TVEV+EEN  RG  +RCRQMRA+EEIGSC EYL QQS
Sbjct: 4   LTSIIALLALALVIADAHRTTITTVEVDEENP-RG-MERCRQMRAREEIGSCGEYLSQQS 63

Query: 67  RYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQLFQ 126
           RYVL+MRGI+NQRRRGG  F+ECCREL NV+EECRCELL+EIA  E+RK  G E  Q+ Q
Sbjct: 64  RYVLQMRGIDNQRRRGGQAFDECCRELMNVEEECRCELLQEIAMEEQRKARGQERMQMLQ 123

Query: 127 RARNLPSMCGIRPQQCYF 145
            ARNLPSMCG RPQQCYF
Sbjct: 124 TARNLPSMCGTRPQQCYF 139

BLAST of Lsi02G015220 vs. ExPASy TrEMBL
Match: A0A6J1JVR2 (2S albumin OS=Cucurbita maxima OX=3661 GN=LOC111489309 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 3.6e-39
Identity = 94/140 (67.14%), Postives = 113/140 (80.71%), Query Frame = 0

Query: 7   IRTIIALLAAALVVAD--AHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQ 66
           + +IIAL A AL+VAD  A+RT I TVEVEE  QGR  ++RCRQM A+EE+ SC++YLRQ
Sbjct: 4   LTSIIALFAVALLVADAYAYRTTITTVEVEENRQGR--EERCRQMSAREELRSCEKYLRQ 63

Query: 67  QSRYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQL 126
           QSR VL+MRGIEN  RR G  F+ECCRELRNVDEECRC++LEEIA  E+R+  G E RQ+
Sbjct: 64  QSRDVLQMRGIENPWRREGGSFDECCRELRNVDEECRCDMLEEIAREEQRQARGQEGRQM 123

Query: 127 FQRARNLPSMCGIRPQQCYF 145
            Q+ARNLPSMCGIRPQ+C F
Sbjct: 124 LQKARNLPSMCGIRPQRCDF 141

BLAST of Lsi02G015220 vs. ExPASy TrEMBL
Match: A0A6J1EW73 (2S albumin-like OS=Cucurbita moschata OX=3662 GN=LOC111438467 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.4e-38
Identity = 92/136 (67.65%), Postives = 109/136 (80.15%), Query Frame = 0

Query: 10  IIALLAAALVVADAHR-TIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQQSRY 69
           IIAL+  A++V+DA+    I TVEV ++N+ R   +RC QMRA EEIGSC +YL QQSR 
Sbjct: 7   IIALILMAVLVSDAYAYRTITTVEVNKDNERRA--ERCSQMRADEEIGSCVQYLTQQSRD 66

Query: 70  VLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQLFQRA 129
           VL+MRGIENQRRR G +F+ECCRELRNVDE+CRCELLE+IA  E RKG   E RQ+ QRA
Sbjct: 67  VLQMRGIENQRRREGQVFDECCRELRNVDEQCRCELLEQIAMWETRKGRPQETRQMQQRA 126

Query: 130 RNLPSMCGIRPQQCYF 145
           RNLPSMCG+RPQQCYF
Sbjct: 127 RNLPSMCGLRPQQCYF 140

BLAST of Lsi02G015220 vs. ExPASy TrEMBL
Match: A0A6J1FV75 (2S albumin OS=Cucurbita moschata OX=3662 GN=LOC111447584 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.8e-38
Identity = 93/140 (66.43%), Postives = 112/140 (80.00%), Query Frame = 0

Query: 7   IRTIIALLAAALVVAD--AHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQ 66
           + +IIAL A AL+VAD  A+RT I TVEVEE    RG ++RCRQM A+EE+ SC++YLRQ
Sbjct: 4   LTSIIALFAVALLVADAYAYRTTITTVEVEENR--RGGEERCRQMSAREELRSCEQYLRQ 63

Query: 67  QSRYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQL 126
           QSR VL+MRGIEN  RR G  F+ECCRELRNVDEECRC++LEEIA  E+R+  G E RQ+
Sbjct: 64  QSREVLQMRGIENPWRREGRSFDECCRELRNVDEECRCDMLEEIAREEQRQARGQEGRQM 123

Query: 127 FQRARNLPSMCGIRPQQCYF 145
            Q+ARNLPSMCGIRPQ+C F
Sbjct: 124 LQKARNLPSMCGIRPQRCDF 141

BLAST of Lsi02G015220 vs. NCBI nr
Match: XP_038889520.1 (2S albumin-like [Benincasa hispida])

HSP 1 Score: 209.9 bits (533), Expect = 1.4e-50
Identity = 112/146 (76.71%), Postives = 119/146 (81.51%), Query Frame = 0

Query: 1   MGRSVVIRTIIALLAAALVVADAHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQE 60
           M +   I  I AL    LVVADAHRTII T+EVEEE   + Q +RCRQMRA+EEIG C E
Sbjct: 1   MAKLTSIVIIAALGVLGLVVADAHRTIITTMEVEEEEYNQRQHERCRQMRAREEIGRCAE 60

Query: 61  YLRQQSR--YVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGG 120
           YL QQSR  YVLEMRGIENQRRRGG+L NECC ELRNVDEECRCELLEEI  MERRKGGG
Sbjct: 61  YLTQQSRRPYVLEMRGIENQRRRGGELLNECCNELRNVDEECRCELLEEIVSMERRKGGG 120

Query: 121 PEERQLFQRARNLPSMCGIRPQQCYF 145
            EERQ+FQRARNLPSMCGIRPQQCYF
Sbjct: 121 QEERQMFQRARNLPSMCGIRPQQCYF 146

BLAST of Lsi02G015220 vs. NCBI nr
Match: XP_011650534.1 (2S albumin [Cucumis sativus])

HSP 1 Score: 191.8 bits (486), Expect = 4.1e-45
Identity = 99/138 (71.74%), Postives = 116/138 (84.06%), Query Frame = 0

Query: 7   IRTIIALLAAALVVADAHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQQS 66
           + +IIALLA ALV+ADAHRT I TVEV+EEN GR   +RCRQMRA+EEIGSC +YL QQS
Sbjct: 4   LTSIIALLALALVIADAHRTTITTVEVDEENPGR--MERCRQMRAREEIGSCGQYLSQQS 63

Query: 67  RYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQLFQ 126
           RYVL+MRGI+NQRRRGG LF+ECC EL NV+EECRCELL+EIA  E+R+  G E  Q+ Q
Sbjct: 64  RYVLQMRGIDNQRRRGGQLFDECCSELTNVEEECRCELLQEIAMEEQRRARGQERTQMLQ 123

Query: 127 RARNLPSMCGIRPQQCYF 145
            A+NLPSMCG+RPQQCYF
Sbjct: 124 TAKNLPSMCGMRPQQCYF 139

BLAST of Lsi02G015220 vs. NCBI nr
Match: KAA0025475.1 (2S albumin precursor [Cucumis melo var. makuwa] >TYK00390.1 hypothetical protein E5676_scaffold1112G00590 [Cucumis melo var. makuwa])

HSP 1 Score: 190.7 bits (483), Expect = 9.0e-45
Identity = 102/138 (73.91%), Postives = 115/138 (83.33%), Query Frame = 0

Query: 7   IRTIIALLAAALVVADAHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQQS 66
           + +IIALLA ALV+ADAHRT I TVEV+EEN  RG  +RCRQMRA+EEIGSC EYL QQS
Sbjct: 4   LTSIIALLALALVIADAHRTTITTVEVDEENP-RG-MERCRQMRAREEIGSCGEYLSQQS 63

Query: 67  RYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQLFQ 126
           RYVL+MRGI+NQRRRGG  F+ECCREL NV+EECRCELL+EIA  E+RK  G E  Q+ Q
Sbjct: 64  RYVLQMRGIDNQRRRGGQAFDECCRELMNVEEECRCELLQEIAMEEQRKARGQERMQMLQ 123

Query: 127 RARNLPSMCGIRPQQCYF 145
            ARNLPSMCG RPQQCYF
Sbjct: 124 TARNLPSMCGTRPQQCYF 139

BLAST of Lsi02G015220 vs. NCBI nr
Match: XP_038904176.1 (2S albumin-like [Benincasa hispida])

HSP 1 Score: 181.4 bits (459), Expect = 5.5e-42
Identity = 99/135 (73.33%), Postives = 111/135 (82.22%), Query Frame = 0

Query: 10  IIALLAAALVVADAHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQQSRYV 69
           I A LA AL+VADAHRT I TVEV+E+NQGR   +RCRQMRA+EEIGSC +YL QQSRY 
Sbjct: 7   IFAFLAVALLVADAHRTTITTVEVDEDNQGR--MERCRQMRAREEIGSCAQYLSQQSRY- 66

Query: 70  LEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQLFQRAR 129
           L+MRGI+NQRRR G  F ECC ELRNVDEECRCELLEEIA  E+R+  G +  Q+ QRAR
Sbjct: 67  LQMRGIDNQRRREGQAFEECCSELRNVDEECRCELLEEIAREEQRRARG-QGTQMMQRAR 126

Query: 130 NLPSMCGIRPQQCYF 145
           NLPSMCGIRPQQCYF
Sbjct: 127 NLPSMCGIRPQQCYF 137

BLAST of Lsi02G015220 vs. NCBI nr
Match: XP_022993226.1 (2S albumin [Cucurbita maxima])

HSP 1 Score: 171.0 bits (432), Expect = 7.4e-39
Identity = 94/140 (67.14%), Postives = 113/140 (80.71%), Query Frame = 0

Query: 7   IRTIIALLAAALVVAD--AHRTIIRTVEVEEENQGRGQQQRCRQMRAQEEIGSCQEYLRQ 66
           + +IIAL A AL+VAD  A+RT I TVEVEE  QGR  ++RCRQM A+EE+ SC++YLRQ
Sbjct: 4   LTSIIALFAVALLVADAYAYRTTITTVEVEENRQGR--EERCRQMSAREELRSCEKYLRQ 63

Query: 67  QSRYVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGGPEERQL 126
           QSR VL+MRGIEN  RR G  F+ECCRELRNVDEECRC++LEEIA  E+R+  G E RQ+
Sbjct: 64  QSRDVLQMRGIENPWRREGGSFDECCRELRNVDEECRCDMLEEIAREEQRQARGQEGRQM 123

Query: 127 FQRARNLPSMCGIRPQQCYF 145
            Q+ARNLPSMCGIRPQ+C F
Sbjct: 124 LQKARNLPSMCGIRPQRCDF 141

BLAST of Lsi02G015220 vs. TAIR 10
Match: AT4G27140.1 (seed storage albumin 1 )

HSP 1 Score: 47.0 bits (110), Expect = 1.5e-05
Identity = 37/141 (26.24%), Postives = 76/141 (53.90%), Query Frame = 0

Query: 10  IIALLAAALVVADA--HRTIIRTVEVEEENQGRGQQQRCR-QMRAQEEIGSCQEYLRQQS 69
           + A LA   ++ +A  +RT++   E +  N    + ++CR + + ++ + +CQ+ + QQ+
Sbjct: 8   VCAALALCFLLTNASIYRTVVEFEEDDATNPIGPKMRKCRKEFQKEQHLRACQQLMLQQA 67

Query: 70  R--------YVLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKGGG 129
           R        +  +M   + Q++    LF +CC ELR  + +C C  L++ A   R +G  
Sbjct: 68  RQGRSDEFDFEDDMENPQGQQQE-QQLFQQCCNELRQEEPDCVCPTLKQAAKAVRLQGQH 127

Query: 130 P--EERQLFQRARNLPSMCGI 138
              + R+++Q A++LP++C I
Sbjct: 128 QPMQVRKIYQTAKHLPNVCDI 147

BLAST of Lsi02G015220 vs. TAIR 10
Match: AT5G54740.1 (seed storage albumin 5 )

HSP 1 Score: 44.3 bits (103), Expect = 9.7e-05
Identity = 45/150 (30.00%), Postives = 73/150 (48.67%), Query Frame = 0

Query: 8   RTIIALLAAALVVADAHRTIIRT-VEVEEENQ-GRGQQQRC-RQMRAQEEIGSCQEYLR- 67
           + I+     AL +  A+ +I RT VE EE++     QQ +C R+    +++  C++++R 
Sbjct: 3   KLILVFATLALFILLANASIYRTVVEFEEDDDVSNPQQGKCQREFMKHQQLRGCKQWIRK 62

Query: 68  --QQSRYVLEMRGIE---------NQRRRG---GDLFNECCRELRNVDEECRCELLEEIA 127
             QQ R   E    E         ++   G         CC ELR VD+ C C  L++ A
Sbjct: 63  RAQQGRIGYEADDFELTLDVDLEDDENPMGPQQQSSLKMCCNELRQVDKMCVCPTLKKAA 122

Query: 128 GMERRKG--GGPEERQLFQRARNLPSMCGI 138
              R +G  G  + + +FQ A+NLP++C I
Sbjct: 123 QQVRFQGMHGQQQVQHVFQTAKNLPNVCKI 152

BLAST of Lsi02G015220 vs. TAIR 10
Match: AT4G27160.1 (seed storage albumin 3 )

HSP 1 Score: 41.2 bits (95), Expect = 8.2e-04
Identity = 40/141 (28.37%), Postives = 73/141 (51.77%), Query Frame = 0

Query: 10  IIALLAAALVVADAHRTIIRT-VEVEEENQGR--GQQQRC-RQMRAQEEIGSCQEYLRQQ 69
           + A LA   ++ +A  +I RT VE EE++     G +QRC ++ +  + + +CQ ++ +Q
Sbjct: 8   VCATLALCFLLTNA--SIYRTVVEFEEDDASNPVGPRQRCQKEFQQSQHLRACQRWMSKQ 67

Query: 70  SRY------VLEMRGIENQRRRGGDLFNECCRELRNVDEECRCELLEEIAGMERRKG--G 129
            R        L+        ++G  L  +CC ELR  +  C C  L++ A     +G  G
Sbjct: 68  MRQGRGGGPSLDDEFDFEGPQQGYQLLQQCCNELRQEEPVCVCPTLKQAARAVSLQGQHG 127

Query: 130 GPEERQLFQRARNLPSMCGIR 139
             + R+++Q A+ LP++C I+
Sbjct: 128 PFQSRKIYQSAKYLPNICKIQ 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q396491.7e-4166.432S albumin OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
P931983.3e-1338.692S albumin seed storage protein (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1[more]
P0C8Y81.2e-1037.412S sulfur-rich seed storage protein 2 OS=Bertholletia excelsa OX=3645 GN=BE2S2 P... [more]
P044032.6e-1035.662S sulfur-rich seed storage protein 1 OS=Bertholletia excelsa OX=3645 GN=BE2S1 P... [more]
P154613.7e-0934.432S seed storage protein OS=Helianthus annuus OX=4232 GN=HAG5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L7Q22.0e-4571.74AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G080340 PE=4 SV... [more]
A0A5A7SLZ04.4e-4573.912S albumin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1112G00590 PE... [more]
A0A6J1JVR23.6e-3967.142S albumin OS=Cucurbita maxima OX=3661 GN=LOC111489309 PE=4 SV=1[more]
A0A6J1EW731.4e-3867.652S albumin-like OS=Cucurbita moschata OX=3662 GN=LOC111438467 PE=4 SV=1[more]
A0A6J1FV751.8e-3866.432S albumin OS=Cucurbita moschata OX=3662 GN=LOC111447584 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038889520.11.4e-5076.712S albumin-like [Benincasa hispida][more]
XP_011650534.14.1e-4571.742S albumin [Cucumis sativus][more]
KAA0025475.19.0e-4573.912S albumin precursor [Cucumis melo var. makuwa] >TYK00390.1 hypothetical protein... [more]
XP_038904176.15.5e-4273.332S albumin-like [Benincasa hispida][more]
XP_022993226.17.4e-3967.142S albumin [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G27140.11.5e-0526.24seed storage albumin 1 [more]
AT5G54740.19.7e-0530.00seed storage albumin 5 [more]
AT4G27160.18.2e-0428.37seed storage albumin 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000617Napin/ Bra allergenPRINTSPR00496NAPINcoord: 88..105
score: 40.28
coord: 121..135
score: 34.67
coord: 44..65
score: 18.64
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainSMARTSM00499aai_6coord: 58..142
e-value: 3.8E-9
score: 46.4
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainPFAMPF00234Tryp_alpha_amylcoord: 58..142
e-value: 8.5E-9
score: 35.7
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 39..144
e-value: 1.1E-20
score: 76.0
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 12..143
NoneNo IPR availablePANTHERPTHR35496:SF42S SEED STORAGE PROTEIN 1-RELATEDcoord: 9..144
NoneNo IPR availablePANTHERPTHR354962S SEED STORAGE PROTEIN 1-RELATEDcoord: 9..144
IPR044723AAI/SS protein, conserved domainCDDcd00261AAI_SScoord: 41..144
e-value: 4.58514E-6
score: 41.2343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G015220.1Lsi02G015220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
cellular_component GO:0033095 aleurone grain
cellular_component GO:0000322 storage vacuole
molecular_function GO:0015066 alpha-amylase inhibitor activity
molecular_function GO:0045735 nutrient reservoir activity