Lsi02G010810 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G010810
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionUbiquinol oxidase
Locationchr02: 12071344 .. 12074743 (-)
RNA-Seq ExpressionLsi02G010810
SyntenyLsi02G010810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAAAATAATAAAACTTGATTGAGGTTGGCAAACAAGGGAAATGGCCTCGGGAAAGTACCAACTGCCGATTAACAATTCTTCTCCGGTTCATCCTCATACTTCCCCCACTTTCTCTTTCACCATTCCAACCTTGATTTCCAAATTCTCTCCCTCGCTCCGAAGCTATGTCCACTGCTTGGTCTTCTTCCTCTTCCGCAGTTTTTGCTATCTCCTCCGTCGAATTGAAGCCACACAACACCTACAACCTCGCCTCCTTCAGAATCTCCACTTCCTTCCCTCGGCGTTCCTTCTTTGCACGCCCTCTAATTTCCAGGTAAGAGTTCCTCTAAATGTTTCTTTAACAACTACAGCTTTGATTTTATCTGCTTCCTTGATTTCCATATCTTGTGTTTGTATAAATGTCTCTTGGTGAGCTGAAAAGGGTTTTTCTACGACTCAGTTTTGGGATTGATTTCGGAGTTTCTAAGCCCGATTTCATTTTGCTTTCTTTGATAAATTGTTGAGAGAATGTTTGATTCTATCATTTTTATCATTTTTCTTTTCACATCGATTGTGATTGGTATCTGATGTAGAAAATCATGTCGGGTGCTGGCAACAGTTCTAAAGGAAGATGAGGAAAAAGTGGTTGTGGAGGATTCATTTCCTCCTAAGGCTTCTCCTGTTCAAGACGGGGAAGGAGGCAGTAACCAAGGACCAGAAAATTCATCCTCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTGTTAATGTCCTTCTCACGGTAATCTCAGTTCAAACTGATCACTGCTCTTCATTTGTGCAAAATTGGTTCTGCAGTACTTGATATGTTGAATCATGACTCCTCTTTATTAATCTTTCTAGGATTCAGTAATCAGAATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTGTTGGAAACTATTGCAAGGGTTCCCTATTTTGGTGAGCTTTTAATTACTTCAAGCCTACCACTTTGTTAGGACCTTCTTTTTTATGTCTTCCTTGAAACAGAGCTAATCTGTAATTAGCTTAAGAAGGGTGGGACTTTTAGGTTTTGTTTGGTATCTTATCCGCATTTTGTTTTTCTGATTTCAGATTCACTTAATCAAATGGATTTAATGAATATCCGTTTGACAGATTTGTTTTTTTTTTCCTTTCTTTTTGGATTTTGTGATCCTAAACACATAATCAGAAACAGTGAAAACATAAAATATAACTCTGATCTTTATTGAATCCCTTGTTTTCAAATTTATGATTTTCGATCACTAACCAAAAGTTATTCTCTTCTCATTAACTGCAAAGTTTGTTATCTAAATGCAATTTGGCAGGCTATAAGAACAATTTTATGTCACCTATTGAAGAACATTCTCATTAACACAAAATTGGATTCTCTAATAAATATGCAGATTGGTTTTTAGTTTAAAATAATTGGTTCTGCTACTAGTTTAATTTTGGAGCTAGATTGTACAGCTTCTGTGGTTATTTATTTGAGGACGATTACTGTAGTGTCTAGTTTGCAGTTGTTTCTAATTTCTTCCTTTTAAATTGCAGCGTTTCTATCAGTTCTACATATGTATGAAAGTTTTGGCTGGTGGAGAAGAGCTGATTATCTGAAAGTTCATTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGTACTTATTATCTTGCCATGATTTCATCCTGGTTAGAGCTGGAAGTATTCCCCTTTTTGTTTGCCCTAGTATGTTGAAGGGGACTGTATTGGTGATAGATGAAGGCTTTGTAAGAATTGCCTTTCTTACAAGATTTTCCTTTTCAATTATCTTGTGAACTGCATGTTTTATTTGGTGAACCCGTCTAGACGTCTAATTATTGAATAAAATAACTCCTTTTCTGACTTGATTCCAGGAACTGGGGGGCAACGATTGGTGGTTTGATCGTTTTCTTGCTCAACATATTGCTGTTGCTTATTATTTTATGACAGTACTTATGTACATGATTAGCCCAAGAATGGCATGTAAGTATTGCTAGAGCTGATAAATAAAACTGCTTATACTCTCTTTCTTGCATCCTTTTGTGGGCTTGTTCAGTTCACTCTTAAATTGTAAGAAGTTACCATGAAAACAGAGAATTGGCTTGGCTTTCCTTCTTAGTAAAACTTACGAAGGAATTAAAATTTAAATTGCAGTTTGGCATCCGTTTTGGTGTAAAACAATAAGAAGTGGCTAGTTACTTTAGGACACCTTTCTATTTTGATGTGCTTTTATCTTCTTCAAGTGTTTTCGTATTAAACTTCTAATGACATCTTTCTCTACAGATCACTTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAATTTCTAAAGGCCCAAGGAGGTATGTTGGTTGCAATCTCGATTTTCTTCTAGTTAACATTATATCTCAAATTAGAATATAATTCTGATATGTTCCATTCCTCGTGTTTATCTATAGAGGAGTTAAAAAAATTGCCTGCTCCCGAGGTTGCTGTGAAGTATTACACTGAAGGAGACTTGTACTTATTTGGTAAGTTCTAGCTTTCTTACCACATGCACAGTTTTTTTTATCAAACTGAGACTAACAAATTGATTTCATTCAGATGAATTTCAGACTTCCAGAGCTCCCAAATCACGAAGACCAAAAATAGGTAAATTTTGAAATCTGGTTTAGTTACTGTAGTTCATAGTGCAAATATCATGATATAGTACAGCCATGAAGGCATGTCTTAAACAAGAAAATGTTCTTTTTCCAAAAAAAATAATAATAACAATAAAGGTTGGCAAAAATAGGAGAAGAGTAGAAGCCCCAAGCATGAGTATGCGGTTCTCTTCTTTCTTTTTAATTGAAGTTTAAGTTCCCTCAGTTTGCTAAATGAGCCCTCAATTTACTGAATGATAAAATAATACGTTATATTTGATGTTGGTGATAGATGGTTTCATTGTGTCTGTGTTGTCTCTATTCGAAAGTTCACTTGACCAAATGTTAATTTGAGGCCATGTTGAGAGTGATTTTGAAATGATTAAAACATTTTTGTCATGTTCAAGATGTTCAAGATCACTCTGTAACATACCTATAATCCTTCAAAATCAATTCAATATTTGATTTGATTTCCCTTTGTTATTCTAGAAAGCTTGTACGATGTGTTTGTGAATGTCAGAGATGACGAAGCTGAGCATTGCAAGACAATGAGAGCTTGTCAATCGCACGGGAATCTCCGGTCACCACATTCTTATTCAGAGAGCTGTCCTGAAGATGATGAGCCACTCTGCAACCTAGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCATTACTTCTTCCCCTCCGGTAAAGCAAAAGCTATGA

mRNA sequence

ACAAAATAATAAAACTTGATTGAGGTTGGCAAACAAGGGAAATGGCCTCGGGAAAGTACCAACTGCCGATTAACAATTCTTCTCCGGTTCATCCTCATACTTCCCCCACTTTCTCTTTCACCATTCCAACCTTGATTTCCAAATTCTCTCCCTCGCTCCGAAGCTATGTCCACTGCTTGGTCTTCTTCCTCTTCCGCAGTTTTTGCTATCTCCTCCGTCGAATTGAAGCCACACAACACCTACAACCTCGCCTCCTTCAGAATCTCCACTTCCTTCCCTCGGCGTTCCTTCTTTGCACGCCCTCTAATTTCCAGAAAATCATGTCGGGTGCTGGCAACAGTTCTAAAGGAAGATGAGGAAAAAGTGGTTGTGGAGGATTCATTTCCTCCTAAGGCTTCTCCTGTTCAAGACGGGGAAGGAGGCAGTAACCAAGGACCAGAAAATTCATCCTCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTGTTAATGTCCTTCTCACGGATTCAGTAATCAGAATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTGTTGGAAACTATTGCAAGGGTTCCCTATTTTGCGTTTCTATCAGTTCTACATATGTATGAAAGTTTTGGCTGGTGGAGAAGAGCTGATTATCTGAAAGTTCATTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGAACTGGGGGGCAACGATTGGTGGTTTGATCGTTTTCTTGCTCAACATATTGCTGTTGCTTATTATTTTATGACAGTACTTATGTACATGATTAGCCCAAGAATGGCATATCACTTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAATTTCTAAAGGCCCAAGGAGAGGAGTTAAAAAAATTGCCTGCTCCCGAGGTTGCTGTGAAGTATTACACTGAAGGAGACTTGTACTTATTTGATGAATTTCAGACTTCCAGAGCTCCCAAATCACGAAGACCAAAAATAGCTGAGCATTGCAAGACAATGAGAGCTTGTCAATCGCACGGGAATCTCCGGTCACCACATTCTTATTCAGAGAGCTGTCCTGAAGATGATGAGCCACTCTGCAACCTAGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCATTACTTCTTCCCCTCCGGTAAAGCAAAAGCTATGA

Coding sequence (CDS)

ATGTCCACTGCTTGGTCTTCTTCCTCTTCCGCAGTTTTTGCTATCTCCTCCGTCGAATTGAAGCCACACAACACCTACAACCTCGCCTCCTTCAGAATCTCCACTTCCTTCCCTCGGCGTTCCTTCTTTGCACGCCCTCTAATTTCCAGAAAATCATGTCGGGTGCTGGCAACAGTTCTAAAGGAAGATGAGGAAAAAGTGGTTGTGGAGGATTCATTTCCTCCTAAGGCTTCTCCTGTTCAAGACGGGGAAGGAGGCAGTAACCAAGGACCAGAAAATTCATCCTCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTGTTAATGTCCTTCTCACGGATTCAGTAATCAGAATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTGTTGGAAACTATTGCAAGGGTTCCCTATTTTGCGTTTCTATCAGTTCTACATATGTATGAAAGTTTTGGCTGGTGGAGAAGAGCTGATTATCTGAAAGTTCATTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGAACTGGGGGGCAACGATTGGTGGTTTGATCGTTTTCTTGCTCAACATATTGCTGTTGCTTATTATTTTATGACAGTACTTATGTACATGATTAGCCCAAGAATGGCATATCACTTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAATTTCTAAAGGCCCAAGGAGAGGAGTTAAAAAAATTGCCTGCTCCCGAGGTTGCTGTGAAGTATTACACTGAAGGAGACTTGTACTTATTTGATGAATTTCAGACTTCCAGAGCTCCCAAATCACGAAGACCAAAAATAGCTGAGCATTGCAAGACAATGAGAGCTTGTCAATCGCACGGGAATCTCCGGTCACCACATTCTTATTCAGAGAGCTGTCCTGAAGATGATGAGCCACTCTGCAACCTAGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCATTACTTCTTCCCCTCCGGTAAAGCAAAAGCTATGA

Protein sequence

MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVLKEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIRILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLKAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIAEHCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Homology
[Close]
BLAST of Lsi02G010810 vs. ExPASy Swiss-Prot
Match: Q56X52 (Ubiquinol oxidase 4, chloroplastic/chromoplastic OS=Arabidopsis thaliana OX=3702 GN=AOX4 PE=1 SV=2)

HSP 1 Score: 416.8 bits (1070), Expect = 2.4e-115
Identity = 225/352 (63.92%), Postives = 257/352 (73.01%), Query Frame = 0 

Query: 10  SAVFAISSVEL---KPHNTYNLASFRISTSFPRRSFFARPLISRK-----SCRVLATVLK 69
           +A+  ISS  L   +P  T   +   +S S   R     PL SR+     + RV AT+L+
Sbjct: 2   AAISGISSGTLTISRPLVTLRRSRAAVSYSSSHRLLHHLPLSSRRLLLRNNHRVQATILQ 61

Query: 70  EDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIRI 129
           +DEEKVVVE+SF  KA      E        +SS++  + WIIKLEQ VNV LTDSVI+I
Sbjct: 62  DDEEKVVVEESF--KAETSTGTEPLEEPNMSSSSTSAFETWIIKLEQGVNVFLTDSVIKI 121

Query: 130 LDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHL 189
           LD  Y DR YARFFVLETIARVPYFAF+SVLHMYE+FGWWRRADYLKVHFAESWNEMHHL
Sbjct: 122 LDTLYRDRTYARFFVLETIARVPYFAFMSVLHMYETFGWWRRADYLKVHFAESWNEMHHL 181

Query: 190 LIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLK 249
           LIMEELGGN WWFDRFLAQHIA  YYFMTV +Y++SPRMAYHFSECVESHA+ TYDKFLK
Sbjct: 182 LIMEELGGNSWWFDRFLAQHIATFYYFMTVFLYILSPRMAYHFSECVESHAYETYDKFLK 241

Query: 250 AQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AEH 309
           A GEELK +PAP++AVKYYT GDLYLFDEFQTSR P +RRP I              AEH
Sbjct: 242 ASGEELKNMPAPDIAVKYYTGGDLYLFDEFQTSRTPNTRRPVIENLYDVFVNIRDDEAEH 301

Query: 310 CKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL-AEADCEGIVDCIKKSITS 339
           CKTMRACQ+ G+LRSPHS  E    ++E  C +  EA CEGIVDC+KKSITS
Sbjct: 302 CKTMRACQTLGSLRSPHSILEDDDTEEESGCVVPEEAHCEGIVDCLKKSITS 351

[Close]
BLAST of Lsi02G010810 vs. ExPASy Swiss-Prot
Match: Q40294 (Ubiquinol oxidase, mitochondrial OS=Mangifera indica OX=29780 GN=AOMI 1 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.8e-09
Identity = 41/148 (27.70%), Postives = 76/148 (51.35%), Query Frame = 0 

Query: 115 TDSVIRI-LDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAE 174
           T  ++R+  D F+  R+  R  +LET+A VP      +LH+       +   ++K    E
Sbjct: 123 TVKILRVPTDIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSLRKLEQSGGWIKALLEE 182

Query: 175 SWNEMHHLLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAF 234
           + NE  HL+ M EL     W++R L   +   ++    ++Y++SP++A+     +E  A 
Sbjct: 183 AENERMHLMTMVEL-VQPKWYERLLVLAVQGVFFNSFFVLYVLSPKLAHRIVGYLEEEAI 242

Query: 235 STYDKFLK-AQGEELKKLPAPEVAVKYY 261
            +Y ++LK      +K +PAP +A+ Y+
Sbjct: 243 HSYTEYLKDIDSGAIKNIPAPAIAIDYW 269

[Close]
BLAST of Lsi02G010810 vs. ExPASy Swiss-Prot
Match: O22048 (Ubiquinol oxidase 1c, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX1C PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.2e-09
Identity = 44/159 (27.67%), Postives = 77/159 (48.43%), Query Frame = 0 

Query: 123 DAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHLL 182
           D F+  R+  R  +LET+A VP      ++H      + +   ++K    E+ NE  HL+
Sbjct: 143 DLFFQRRYGCRAIMLETVAAVPGMVGGMLMHFKSLRRFEQSGGWIKALLEEAENERMHLM 202

Query: 183 IMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLK- 242
              E+     W++R L   +   ++   ++ Y+ISP+ A+     +E  A  +Y +FLK 
Sbjct: 203 TFMEV-AKPKWYERALVISVQGVFFNAYLIGYIISPKFAHRMVGYLEEEAIHSYTEFLKE 262

Query: 243 AQGEELKKLPAPEVAVKYY-TEGDLYLFDEFQTSRAPKS 280
                ++ +PAP +AV Y+  E D  L D     RA ++
Sbjct: 263 LDNGNIENVPAPAIAVDYWRLEADATLRDVVMVVRADEA 300

[Close]
BLAST of Lsi02G010810 vs. ExPASy Swiss-Prot
Match: Q40578 (Ubiquinol oxidase 2, mitochondrial OS=Nicotiana tabacum OX=4097 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 8.9e-09
Identity = 41/142 (28.87%), Postives = 74/142 (52.11%), Query Frame = 0 

Query: 123 DAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHLL 182
           D F+  R+  R  +LET+A VP      +LH      + +   ++K    E+ NE  HL+
Sbjct: 111 DIFFQRRYGCRAMMLETVAAVPGMVGGMLLHCKSLRRFEQSGGWIKALLEEAENERMHLM 170

Query: 183 IMEELGGNDWWFDR---FLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKF 242
              E+   + W++R   F  Q + +  YF+T   Y++SP++A+     +E  A  +Y +F
Sbjct: 171 TFMEVAKPN-WYERALVFAVQGVFINAYFVT---YLLSPKLAHRIVGYLEEEAIHSYTEF 230

Query: 243 LKAQGE-ELKKLPAPEVAVKYY 261
           LK   +  ++ +PAP +A+ Y+
Sbjct: 231 LKELDKGNIENVPAPAIAIDYW 248

[Close]
BLAST of Lsi02G010810 vs. ExPASy Swiss-Prot
Match: O22049 (Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-08
Identity = 40/139 (28.78%), Postives = 70/139 (50.36%), Query Frame = 0 

Query: 123 DAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHLL 182
           D F+  R+  R  +LET+A VP      +LH+     +     ++K    E+ NE  HL+
Sbjct: 167 DIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLM 226

Query: 183 IMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLK- 242
            M EL     W++R L   +   ++    + Y+ISPR+A+     +E  A  +Y +FLK 
Sbjct: 227 TMMEL-VKPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKD 286

Query: 243 AQGEELKKLPAPEVAVKYY 261
               +++ + AP +A+ Y+
Sbjct: 287 IDNGKIENVAAPAIAIDYW 304

[Close]
BLAST of Lsi02G010810 vs. ExPASy TrEMBL
Match: A0A5A7UI87 (Ubiquinol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00950 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.1e-178
Identity = 321/360 (89.17%), Postives = 331/360 (91.94%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS A SSSSSAVFAISSVELKP NT N+ SFRI TSF      ARPLISRKSCRV+ATVL
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEEKVV+E+SFPPKASPVQDGEGGS QGP++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYHFSECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI              AE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 347
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

[Close]
BLAST of Lsi02G010810 vs. ExPASy TrEMBL
Match: A0A1S3C992 (Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103498237 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.1e-178
Identity = 321/360 (89.17%), Postives = 331/360 (91.94%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS A SSSSSAVFAISSVELKP NT N+ SFRI TSF      ARPLISRKSCRV+ATVL
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEEKVV+E+SFPPKASPVQDGEGGS QGP++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYHFSECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI              AE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 347
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

[Close]
BLAST of Lsi02G010810 vs. ExPASy TrEMBL
Match: A0A0A0M130 (Ubiquinol oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G699610 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.8e-177
Identity = 318/360 (88.33%), Postives = 329/360 (91.39%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS A SSSSS +FA SSVELKP NT NLASFRI TSF      ARPLISRKSCRV+ATVL
Sbjct: 1   MSIALSSSSSPLFAFSSVELKPINTKNLASFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEEKVV+E+SFPPKASP+QDGEGGS QGPE+SSSNG DKWI+KLEQSVNVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPIQDGEGGSGQGPESSSSNGFDKWIVKLEQSVNVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYHFSECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI              AE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFMNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 347
           HCKTMRACQSHGNLRSPHSYSESCPE+DEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEEDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

[Close]
BLAST of Lsi02G010810 vs. ExPASy TrEMBL
Match: A0A6J1K2B2 (Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111490026 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 4.9e-175
Identity = 312/361 (86.43%), Postives = 327/361 (90.58%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS AW+SSSSA+F  SSV+L P+NT N+ASFRI T+F RRSF +RPL+SRK CRVLATVL
Sbjct: 1   MSIAWTSSSSALFGTSSVQLNPYNTKNIASFRIGTAFSRRSFSSRPLVSRKLCRVLATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEE+VV+E+SFPPKASPV  GEGGS  G ENSSSN LDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVDRGEGGSEGGAENSSSNALDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV+MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKI              AE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIDNLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 347
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL E ADCEGIVDC+KK++TSSPPVKQK
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKQK 360

[Close]
BLAST of Lsi02G010810 vs. ExPASy TrEMBL
Match: A0A6J1GSZ7 (Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457254 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.8e-174
Identity = 312/361 (86.43%), Postives = 327/361 (90.58%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS AW SSSSA+F  SSV+LKP+NT N+ SFRI T+F RRSF +RPL+SRKSCRVLATVL
Sbjct: 1   MSIAWCSSSSALFGTSSVQLKPYNTINIDSFRIGTAFSRRSFSSRPLVSRKSCRVLATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEE+VV+E+SFPPKASPV+ GEGGS  G ENSSSNGLDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVEKGEGGSEGGAENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV+MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKI              AE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIENLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 347
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK++TSSPPVK K
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESRPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKPK 360

[Close]
BLAST of Lsi02G010810 vs. NCBI nr
Match: XP_038893742.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Benincasa hispida])

HSP 1 Score: 653.7 bits (1685), Expect = 9.0e-184
Identity = 329/360 (91.39%), Postives = 334/360 (92.78%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MSTAWSS SSAVFAISSV+LKP+NT N+ASFRI TSFPRRSF A PLISRKSCRVLATVL
Sbjct: 1   MSTAWSSLSSAVFAISSVQLKPNNTKNVASFRIPTSFPRRSFVAHPLISRKSCRVLATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEEKVVVEDSFPPKASPVQDGEGGSN+GPE S SNG DKWIIKLEQSVNV LTDSVIR
Sbjct: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNEGPEISPSNGFDKWIIKLEQSVNVFLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYHFSECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI              AE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 347
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL+EADCEGIVDCIKKSIT S PVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLSEADCEGIVDCIKKSITFSHPVKQKL 360

[Close]
BLAST of Lsi02G010810 vs. NCBI nr
Match: XP_008458986.1 (PREDICTED: ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Cucumis melo] >KAA0054287.1 ubiquinol oxidase 4 [Cucumis melo var. makuwa] >TYK12325.1 ubiquinol oxidase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 634.8 bits (1636), Expect = 4.3e-178
Identity = 321/360 (89.17%), Postives = 331/360 (91.94%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS A SSSSSAVFAISSVELKP NT N+ SFRI TSF      ARPLISRKSCRV+ATVL
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEEKVV+E+SFPPKASPVQDGEGGS QGP++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYHFSECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI              AE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 347
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

[Close]
BLAST of Lsi02G010810 vs. NCBI nr
Match: XP_004145426.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucumis sativus])

HSP 1 Score: 629.8 bits (1623), Expect = 1.4e-176
Identity = 318/360 (88.33%), Postives = 329/360 (91.39%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS A SSSSS +FA SSVELKP NT NLASFRI TSF      ARPLISRKSCRV+ATVL
Sbjct: 1   MSIALSSSSSPLFAFSSVELKPINTKNLASFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEEKVV+E+SFPPKASP+QDGEGGS QGPE+SSSNG DKWI+KLEQSVNVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPIQDGEGGSGQGPESSSSNGFDKWIVKLEQSVNVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV MYMISPRMAYHFSECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI              AE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFMNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 347
           HCKTMRACQSHGNLRSPHSYSESCPE+DEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEEDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

[Close]
BLAST of Lsi02G010810 vs. NCBI nr
Match: XP_022994224.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita maxima])

HSP 1 Score: 623.6 bits (1607), Expect = 1.0e-174
Identity = 312/361 (86.43%), Postives = 327/361 (90.58%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS AW+SSSSA+F  SSV+L P+NT N+ASFRI T+F RRSF +RPL+SRK CRVLATVL
Sbjct: 1   MSIAWTSSSSALFGTSSVQLNPYNTKNIASFRIGTAFSRRSFSSRPLVSRKLCRVLATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEE+VV+E+SFPPKASPV  GEGGS  G ENSSSN LDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVDRGEGGSEGGAENSSSNALDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV+MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKI              AE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIDNLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 347
           HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL E ADCEGIVDC+KK++TSSPPVKQK
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKQK 360

[Close]
BLAST of Lsi02G010810 vs. NCBI nr
Match: XP_022955227.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita moschata])

HSP 1 Score: 621.7 bits (1602), Expect = 3.8e-174
Identity = 312/361 (86.43%), Postives = 327/361 (90.58%), Query Frame = 0 

Query: 1   MSTAWSSSSSAVFAISSVELKPHNTYNLASFRISTSFPRRSFFARPLISRKSCRVLATVL 60
           MS AW SSSSA+F  SSV+LKP+NT N+ SFRI T+F RRSF +RPL+SRKSCRVLATVL
Sbjct: 1   MSIAWCSSSSALFGTSSVQLKPYNTINIDSFRIGTAFSRRSFSSRPLVSRKSCRVLATVL 60

Query: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KEDEE+VV+E+SFPPKASPV+ GEGGS  G ENSSSNGLDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVEKGEGGSEGGAENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFL 240
           LLIMEELGGNDWWFDRFLAQHIAVAYYFMTV+MYMISPRMAYH SECVESHAFSTYDKFL
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAP SRRPKI              AE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIENLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAE-ADCEGIVDCIKKSITSSPPVKQK 347
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK++TSSPPVK K
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESRPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKPK 360

[Close]
BLAST of Lsi02G010810 vs. TAIR 10
Match: AT4G22260.1 (Alternative oxidase family protein )

HSP 1 Score: 416.8 bits (1070), Expect = 1.7e-116
Identity = 225/352 (63.92%), Postives = 257/352 (73.01%), Query Frame = 0 

Query: 10  SAVFAISSVEL---KPHNTYNLASFRISTSFPRRSFFARPLISRK-----SCRVLATVLK 69
           +A+  ISS  L   +P  T   +   +S S   R     PL SR+     + RV AT+L+
Sbjct: 2   AAISGISSGTLTISRPLVTLRRSRAAVSYSSSHRLLHHLPLSSRRLLLRNNHRVQATILQ 61

Query: 70  EDEEKVVVEDSFPPKASPVQDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIRI 129
           +DEEKVVVE+SF  KA      E        +SS++  + WIIKLEQ VNV LTDSVI+I
Sbjct: 62  DDEEKVVVEESF--KAETSTGTEPLEEPNMSSSSTSAFETWIIKLEQGVNVFLTDSVIKI 121

Query: 130 LDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHL 189
           LD  Y DR YARFFVLETIARVPYFAF+SVLHMYE+FGWWRRADYLKVHFAESWNEMHHL
Sbjct: 122 LDTLYRDRTYARFFVLETIARVPYFAFMSVLHMYETFGWWRRADYLKVHFAESWNEMHHL 181

Query: 190 LIMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLK 249
           LIMEELGGN WWFDRFLAQHIA  YYFMTV +Y++SPRMAYHFSECVESHA+ TYDKFLK
Sbjct: 182 LIMEELGGNSWWFDRFLAQHIATFYYFMTVFLYILSPRMAYHFSECVESHAYETYDKFLK 241

Query: 250 AQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKI--------------AEH 309
           A GEELK +PAP++AVKYYT GDLYLFDEFQTSR P +RRP I              AEH
Sbjct: 242 ASGEELKNMPAPDIAVKYYTGGDLYLFDEFQTSRTPNTRRPVIENLYDVFVNIRDDEAEH 301

Query: 310 CKTMRACQSHGNLRSPHSYSESCPEDDEPLCNL-AEADCEGIVDCIKKSITS 339
           CKTMRACQ+ G+LRSPHS  E    ++E  C +  EA CEGIVDC+KKSITS
Sbjct: 302 CKTMRACQTLGSLRSPHSILEDDDTEEESGCVVPEEAHCEGIVDCLKKSITS 351

[Close]
BLAST of Lsi02G010810 vs. TAIR 10
Match: AT3G27620.1 (alternative oxidase 1C )

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-10
Identity = 44/159 (27.67%), Postives = 77/159 (48.43%), Query Frame = 0 

Query: 123 DAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHLL 182
           D F+  R+  R  +LET+A VP      ++H      + +   ++K    E+ NE  HL+
Sbjct: 143 DLFFQRRYGCRAIMLETVAAVPGMVGGMLMHFKSLRRFEQSGGWIKALLEEAENERMHLM 202

Query: 183 IMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLK- 242
              E+     W++R L   +   ++   ++ Y+ISP+ A+     +E  A  +Y +FLK 
Sbjct: 203 TFMEV-AKPKWYERALVISVQGVFFNAYLIGYIISPKFAHRMVGYLEEEAIHSYTEFLKE 262

Query: 243 AQGEELKKLPAPEVAVKYY-TEGDLYLFDEFQTSRAPKS 280
                ++ +PAP +AV Y+  E D  L D     RA ++
Sbjct: 263 LDNGNIENVPAPAIAVDYWRLEADATLRDVVMVVRADEA 300

[Close]
BLAST of Lsi02G010810 vs. TAIR 10
Match: AT5G64210.1 (alternative oxidase 2 )

HSP 1 Score: 62.4 bits (150), Expect = 8.3e-10
Identity = 40/139 (28.78%), Postives = 70/139 (50.36%), Query Frame = 0 

Query: 123 DAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHLL 182
           D F+  R+  R  +LET+A VP      +LH+     +     ++K    E+ NE  HL+
Sbjct: 167 DIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLM 226

Query: 183 IMEELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLK- 242
            M EL     W++R L   +   ++    + Y+ISPR+A+     +E  A  +Y +FLK 
Sbjct: 227 TMMEL-VKPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKD 286

Query: 243 AQGEELKKLPAPEVAVKYY 261
               +++ + AP +A+ Y+
Sbjct: 287 IDNGKIENVAAPAIAIDYW 304

[Close]
BLAST of Lsi02G010810 vs. TAIR 10
Match: AT1G32350.1 (alternative oxidase 1D )

HSP 1 Score: 60.1 bits (144), Expect = 4.1e-09
Identity = 37/137 (27.01%), Postives = 68/137 (49.64%), Query Frame = 0 

Query: 125 FYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHHLLIM 184
           F+  +H     +LET+A VP      +LH+     +     ++K    E+ NE  HL+  
Sbjct: 134 FFQRKHMCHAMLLETVAAVPGMVGGMLLHLKSLRRFEHSGGWIKALLEEAENERMHLMTF 193

Query: 185 EELGGNDWWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHFSECVESHAFSTYDKFLK-AQ 244
            EL     W++R +   +   ++    L Y+ISP++A+  +  +E  A ++Y +FLK   
Sbjct: 194 IEL-SQPKWYERAIVFTVQGVFFNAYFLAYVISPKLAHRITGYLEEEAVNSYTEFLKDID 253

Query: 245 GEELKKLPAPEVAVKYY 261
             + +  PAP +A+ Y+
Sbjct: 254 AGKFENSPAPAIAIDYW 269

The following BLAST results are available for this feature:
BLAST of Lsi02G010810 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q56X522.4e-11563.92Ubiquinol oxidase 4, chloroplastic/chromoplastic OS=Arabidopsis thaliana OX=3702... [more]
Q402941.8e-0927.70Ubiquinol oxidase, mitochondrial OS=Mangifera indica OX=29780 GN=AOMI 1 PE=1 SV=... [more]
O220485.2e-0927.67Ubiquinol oxidase 1c, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX1C PE=... [more]
Q405788.9e-0928.87Ubiquinol oxidase 2, mitochondrial OS=Nicotiana tabacum OX=4097 GN=AOX2 PE=1 SV=... [more]
O220491.2e-0828.78Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 ... [more]
BLAST of Lsi02G010810 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UI872.1e-17889.17Ubiquinol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00... [more]
A0A1S3C9922.1e-17889.17Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103498237 PE=3 SV=1[more]
A0A0A0M1306.8e-17788.33Ubiquinol oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G699610 PE=3 SV=1[more]
A0A6J1K2B24.9e-17586.43Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111490026 PE=3 SV=1[more]
A0A6J1GSZ71.8e-17486.43Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457254 PE=3 SV=1[more]
BLAST of Lsi02G010810 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038893742.19.0e-18491.39ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Benincasa hispida][more]
XP_008458986.14.3e-17889.17PREDICTED: ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Cucumis melo] ... [more]
XP_004145426.11.4e-17688.33ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucumis sativus][more]
XP_022994224.11.0e-17486.43ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita maxima][more]
XP_022955227.13.8e-17486.43ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita moschata][more]
BLAST of Lsi02G010810 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G22260.11.7e-11663.92Alternative oxidase family protein [more]
AT3G27620.13.7e-1027.67alternative oxidase 1C [more]
AT5G64210.18.3e-1028.78alternative oxidase 2 [more]
AT1G32350.14.1e-0927.01alternative oxidase 1D [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038659Alternative oxidase superfamilyGENE3D1.20.1260.140Alternative oxidasecoord: 61..279
e-value: 2.4E-56
score: 192.7
IPR002680Alternative oxidasePFAMPF01786AOXcoord: 114..261
e-value: 1.1E-30
score: 106.9
IPR002680Alternative oxidasePANTHERPTHR31803ALTERNATIVE OXIDASEcoord: 38..332
IPR002680Alternative oxidaseCDDcd01053AOXcoord: 126..298
e-value: 1.32542E-87
score: 259.062
NoneNo IPR availablePIRSRPIRSR005229-1PIRSR005229-1coord: 128..270
e-value: 6.0E-14
score: 49.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..96
NoneNo IPR availablePANTHERPTHR31803:SF10UBIQUINOL OXIDASE 4, CHLOROPLASTIC/CHROMOPLASTICcoord: 38..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G010810.1Lsi02G010810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010230 alternative respiration
biological_process GO:0016117 carotenoid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0070469 respirasome
cellular_component GO:0009579 thylakoid
molecular_function GO:0009916 alternative oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0102721 ubiquinol:oxygen oxidoreductase activity