Lsi02G010250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G010250
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionO-fucosyltransferase family protein
Locationchr02: 10717247 .. 10718590 (+)
RNA-Seq ExpressionLsi02G010250
SyntenyLsi02G010250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGTATCATCGAATGGTAAACGCTCGATATATTGCCTTCGCTACTTCAATTCAAGAATTTGCAAATGCATTGCCATATTACTTATTATTCCACTGATTGTTAGAGCTATTTTCTTCCCGTCGATCTCGAGTTTTGGTCGGATCGAAGAGAATGGCTTAGTAGTCGTCCGTAATCTGTCTCCATTGTATGGTTCCGATTTCGGTATTCGCATAGACAAGTTTTTGGAGGTCCCTCAGATTGTTTGGGGTTTAAACAATCAAAAGATAGCATTTGCAAGAGCTTGTTTAACTGCAAGAATGTTGAACCGAACTCTTTTGATGCCTAGTTTAAGTGCCTCCCTCTTCTATAAAGAAGTGGAGCGTTTGGAACCAATTTCCTTTGACAAGATCTTTCAATTTGAATTGTTCAATTCTCACTGTAATGGGTTTGTTCGATTGGGTCGTTATATCGATATTTCAAATCGAACAAAACCGATTGAACTGTCAAAAGGAAGCGGAAGGAAGTGGACGATCGAGAGGGATTTGGAACAATTGAAAGAGTATAGCAAGGAACCATTTGATAAGTCAGAGGTGATTAGAATAGTTGGTAAAAATCCATTTCTATGGCATGATCACTGGCCTGTAAAGGACTATGCAAAGGTTTTCGAGTGCTTGGTTTTGGTTGACAAGATAGAGAAAGAAGCAGATAAAGTGATTTCAAGGATTAGAGAAGTAGGATCAGAAGTAAGAAGCAAATTCGACGCCGATGCTAATGGTGTTATATCTGAGAAGTTGTTGCAACCGGTGCCATACGTTGCTGTCCACATGAGAATAGAAATAGACTGGATGATTCATTGTAAGAAGTTAGAGCAGAGATCTAAAATAAACCAAATTTGTAGTAGCAAGGAGGAGATAATGAATAGAGTAGGGAACATTACAGGAATGAAAACGCCAACGGTCGTTTATCTTGCCGTTGCAGATAGTTTACTTAACGATTCTTCGATTTTGAAAGGTTGGAAAGAAGGCTTGCTACCTTTTGAAAAGAAGAAATTAGGCATCGATAAGATCTACAAGAAGTATCCTTACCTCATACAGTCAGCAATCGACTACGAAGTTTGCTTAAGAGCCGATGTGTTCGTTGGAAACAGTTTCTCAACATTTTCGAGTCTTGTAGTTCTCGGGAGAACGCAAAAGCTTATGAAAACAGGGGTCGCAGACCTATGTGGTACAAATGTAAGGTGGCCTTCTTATGCTTATAACATATTAGGAGATGCAAAGGGCCCTCAGAAATGGATATCTAATATGTCTGATATAAGCCTCAAGAAAATTAGCTATGGCTCCAACGATACCTCTTGTCGATAG

mRNA sequence

ATGGAAGTATCATCGAATGGTAAACGCTCGATATATTGCCTTCGCTACTTCAATTCAAGAATTTGCAAATGCATTGCCATATTACTTATTATTCCACTGATTGTTAGAGCTATTTTCTTCCCGTCGATCTCGAGTTTTGGTCGGATCGAAGAGAATGGCTTAGTAGTCGTCCGTAATCTGTCTCCATTGTATGGTTCCGATTTCGGTATTCGCATAGACAAGTTTTTGGAGGTCCCTCAGATTGTTTGGGGTTTAAACAATCAAAAGATAGCATTTGCAAGAGCTTGTTTAACTGCAAGAATGTTGAACCGAACTCTTTTGATGCCTAGTTTAAGTGCCTCCCTCTTCTATAAAGAAGTGGAGCGTTTGGAACCAATTTCCTTTGACAAGATCTTTCAATTTGAATTGTTCAATTCTCACTGTAATGGGTTTGTTCGATTGGGTCGTTATATCGATATTTCAAATCGAACAAAACCGATTGAACTGTCAAAAGGAAGCGGAAGGAAGTGGACGATCGAGAGGGATTTGGAACAATTGAAAGAGTATAGCAAGGAACCATTTGATAAGTCAGAGGTGATTAGAATAGTTGGTAAAAATCCATTTCTATGGCATGATCACTGGCCTGTAAAGGACTATGCAAAGGTTTTCGAGTGCTTGGTTTTGGTTGACAAGATAGAGAAAGAAGCAGATAAAGTGATTTCAAGGATTAGAGAAGTAGGATCAGAAGTAAGAAGCAAATTCGACGCCGATGCTAATGGTGTTATATCTGAGAAGTTGTTGCAACCGGTGCCATACGTTGCTGTCCACATGAGAATAGAAATAGACTGGATGATTCATTGTAAGAAGTTAGAGCAGAGATCTAAAATAAACCAAATTTGTAGTAGCAAGGAGGAGATAATGAATAGAGTAGGGAACATTACAGGAATGAAAACGCCAACGGTCGTTTATCTTGCCGTTGCAGATAGTTTACTTAACGATTCTTCGATTTTGAAAGGTTGGAAAGAAGGCTTGCTACCTTTTGAAAAGAAGAAATTAGGCATCGATAAGATCTACAAGAAGTATCCTTACCTCATACAGTCAGCAATCGACTACGAAGTTTGCTTAAGAGCCGATGTGTTCGTTGGAAACAGTTTCTCAACATTTTCGAGTCTTGTAGTTCTCGGGAGAACGCAAAAGCTTATGAAAACAGGGGTCGCAGACCTATGTGGTACAAATGTAAGGTGGCCTTCTTATGCTTATAACATATTAGGAGATGCAAAGGGCCCTCAGAAATGGATATCTAATATGTCTGATATAAGCCTCAAGAAAATTAGCTATGGCTCCAACGATACCTCTTGTCGATAG

Coding sequence (CDS)

ATGGAAGTATCATCGAATGGTAAACGCTCGATATATTGCCTTCGCTACTTCAATTCAAGAATTTGCAAATGCATTGCCATATTACTTATTATTCCACTGATTGTTAGAGCTATTTTCTTCCCGTCGATCTCGAGTTTTGGTCGGATCGAAGAGAATGGCTTAGTAGTCGTCCGTAATCTGTCTCCATTGTATGGTTCCGATTTCGGTATTCGCATAGACAAGTTTTTGGAGGTCCCTCAGATTGTTTGGGGTTTAAACAATCAAAAGATAGCATTTGCAAGAGCTTGTTTAACTGCAAGAATGTTGAACCGAACTCTTTTGATGCCTAGTTTAAGTGCCTCCCTCTTCTATAAAGAAGTGGAGCGTTTGGAACCAATTTCCTTTGACAAGATCTTTCAATTTGAATTGTTCAATTCTCACTGTAATGGGTTTGTTCGATTGGGTCGTTATATCGATATTTCAAATCGAACAAAACCGATTGAACTGTCAAAAGGAAGCGGAAGGAAGTGGACGATCGAGAGGGATTTGGAACAATTGAAAGAGTATAGCAAGGAACCATTTGATAAGTCAGAGGTGATTAGAATAGTTGGTAAAAATCCATTTCTATGGCATGATCACTGGCCTGTAAAGGACTATGCAAAGGTTTTCGAGTGCTTGGTTTTGGTTGACAAGATAGAGAAAGAAGCAGATAAAGTGATTTCAAGGATTAGAGAAGTAGGATCAGAAGTAAGAAGCAAATTCGACGCCGATGCTAATGGTGTTATATCTGAGAAGTTGTTGCAACCGGTGCCATACGTTGCTGTCCACATGAGAATAGAAATAGACTGGATGATTCATTGTAAGAAGTTAGAGCAGAGATCTAAAATAAACCAAATTTGTAGTAGCAAGGAGGAGATAATGAATAGAGTAGGGAACATTACAGGAATGAAAACGCCAACGGTCGTTTATCTTGCCGTTGCAGATAGTTTACTTAACGATTCTTCGATTTTGAAAGGTTGGAAAGAAGGCTTGCTACCTTTTGAAAAGAAGAAATTAGGCATCGATAAGATCTACAAGAAGTATCCTTACCTCATACAGTCAGCAATCGACTACGAAGTTTGCTTAAGAGCCGATGTGTTCGTTGGAAACAGTTTCTCAACATTTTCGAGTCTTGTAGTTCTCGGGAGAACGCAAAAGCTTATGAAAACAGGGGTCGCAGACCTATGTGGTACAAATGTAAGGTGGCCTTCTTATGCTTATAACATATTAGGAGATGCAAAGGGCCCTCAGAAATGGATATCTAATATGTCTGATATAAGCCTCAAGAAAATTAGCTATGGCTCCAACGATACCTCTTGTCGATAG

Protein sequence

MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNLSPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLKEYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVGSEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIMNRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQSAIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAKGPQKWISNMSDISLKKISYGSNDTSCR
Homology
BLAST of Lsi02G010250 vs. ExPASy Swiss-Prot
Match: Q9MA87 (O-fucosyltransferase 23 OS=Arabidopsis thaliana OX=3702 GN=OFUT23 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.2e-153
Identity = 281/443 (63.43%), Postives = 347/443 (78.33%), Query Frame = 0

Query: 14  LRYFNSRI-CKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNLSPLYGSDFGIRI 73
           LR F+  + CKC+ +L+ I L  RA+   S S    +  + L   R++S    S  GIR 
Sbjct: 10  LRIFSKSVACKCL-VLVGIALFYRALLL-SYSPRNALSNSLLFRDRHMSD-SSSTGGIRT 69

Query: 74  DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIF 133
           DKFLEVPQIVWGLNNQKIAFARACLTARM+NRTLLMPSLSASLFYKEV++L PI FDK+F
Sbjct: 70  DKFLEVPQIVWGLNNQKIAFARACLTARMMNRTLLMPSLSASLFYKEVDKLRPIPFDKVF 129

Query: 134 QFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLKEYSK-EPFDKSE 193
           QFE FNS C+GFVRL R+ D+ NR +  +L KGSGR+WT+ERDLE LK+ ++ E  D+ E
Sbjct: 130 QFERFNSLCSGFVRLSRFSDVKNRAQVFDLEKGSGRRWTVERDLEHLKQSARNESIDEFE 189

Query: 194 VIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVGSEVRSKFDADA 253
           VIR++GKNPFLWHDHWPV+DYAKVFEC+V+VD+I +EADKV+ +IRE G   R+K  +  
Sbjct: 190 VIRLIGKNPFLWHDHWPVEDYAKVFECMVVVDEISREADKVVMKIREAGEAERAKLAS-- 249

Query: 254 NGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIMNRVGNITGMKT 313
                 ++L PVP+VAVHMRIEIDWMIHCKKLEQR KI++ICSSK EI++RVGNI+G+KT
Sbjct: 250 ----KTEILGPVPFVAVHMRIEIDWMIHCKKLEQRKKISEICSSKREILDRVGNISGLKT 309

Query: 314 PTVVYLAVADSLL----NDSSILKGWKEGLLPFEKKKLGI-DKIYKKYPYLIQSAIDYEV 373
           PTV+YLAVAD+LL     DSS+L GW++GL+PFEKKKLG+ ++IY KY YLIQSAIDYEV
Sbjct: 310 PTVLYLAVADTLLEEKEEDSSVLSGWRDGLIPFEKKKLGVKEEIYGKYSYLIQSAIDYEV 369

Query: 374 CLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLC--GTNVRWPSYAYNILGDAKG-PQ 433
           CLRADVFVGNSFSTFSSL+VL RTQK  + G    C  G N +W SYAYN+ G++ G P+
Sbjct: 370 CLRADVFVGNSFSTFSSLIVLERTQKARRLGFMSSCKDGGN-KWRSYAYNLAGESNGVPR 429

Query: 434 KWISNMSDISLKKISYGSNDTSC 447
           +W++NM+  SL+ ISYGSN  SC
Sbjct: 430 RWMTNMTHSSLQAISYGSNSVSC 442

BLAST of Lsi02G010250 vs. ExPASy Swiss-Prot
Match: F4HYR4 (O-fucosyltransferase 15 OS=Arabidopsis thaliana OX=3702 GN=OFUT15 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 7.8e-05
Identity = 57/219 (26.03%), Postives = 99/219 (45.21%), Query Frame = 0

Query: 84  GLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIFQFELFNSHCNG 143
           G+N Q++A     + ARMLN TL++P    S  + +  +     F  I+Q E F  + + 
Sbjct: 232 GINQQRVAVCNIVVVARMLNATLVIPKFMFSDVWTDASQ-----FGDIYQVEHFIKYLSP 291

Query: 144 FVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLKE-----YSKEPFD---KSEVIRI 203
            +R+ + +    ++  +E + GS     +  D++ +KE     Y K       K+ V+  
Sbjct: 292 DIRIVKKLPKELQSLDLE-AIGS-----VVTDIDVMKEAKPGFYMKHILPLLLKNRVVHF 351

Query: 204 VGKNPFLWHDHWPVKDYAKVFEC----LVLVDKIEKEADKVISRIREVGSE--------V 263
           +G    L  D  P +       C    L  V KI++    ++ R+R+ GS         V
Sbjct: 352 LGFGNRLAFDPIPFELQRLRCRCNFHALNFVPKIQETGAILVRRLRDSGSHLAPVDPYLV 411

Query: 264 RSKFDADANGVISEK---LLQPVPYVAVHMRIEIDWMIH 280
             KF   A+ ++ +K   L +   Y+AVH+R EID + H
Sbjct: 412 GPKF---ASFILDKKAGPLHKASKYLAVHLRFEIDMVAH 436

BLAST of Lsi02G010250 vs. ExPASy Swiss-Prot
Match: Q9LIN9 (Protein PECTIC ARABINOGALACTAN SYNTHESIS-RELATED OS=Arabidopsis thaliana OX=3702 GN=PAGR PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.0e-04
Identity = 52/205 (25.37%), Postives = 92/205 (44.88%), Query Frame = 0

Query: 84  GLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIFQFELFNSHCNG 143
           GLN Q+IA   A   A+++N TL++P L     +K+  +     F+ IF  + F  +   
Sbjct: 169 GLNQQRIAICNAVAVAKIMNATLILPVLKQDQIWKDTTK-----FEDIFDVDHFIDYLKD 228

Query: 144 FVRLGRYIDISN-RTKPIELSKGSGRKWTIERDLEQLKEYSKEPFDKSEVI------RIV 203
            VR+ R  DI +  T   EL        +I R ++ + +Y+   F    V+      +I+
Sbjct: 229 DVRIVR--DIPDWFTDKAELFS------SIRRTVKNIPKYAAAQFYIDNVLPRIKEKKIM 288

Query: 204 GKNPF---LWHDHWPVKDYAKV-----FECLVLVDKIEKEADKVISRIREVGSEVRSKFD 263
              PF   L +D+ P ++  ++     +  L  + +IE+ AD ++SR+R           
Sbjct: 289 ALKPFVDRLGYDNVP-QEINRLRCRVNYHALKFLPEIEQMADSLVSRMR----------- 340

Query: 264 ADANGVISEKLLQPVPYVAVHMRIE 274
                    +   P PY+A+H+R E
Sbjct: 349 --------NRTGNPNPYMALHLRFE 340

BLAST of Lsi02G010250 vs. ExPASy TrEMBL
Match: A0A1S4E296 (O-fucosyltransferase family protein OS=Cucumis melo OX=3656 GN=LOC103498309 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.1e-231
Identity = 401/446 (89.91%), Postives = 421/446 (94.39%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           MEVSSNG+RSIYCLR+ N +I KC+A LL++ LI R I FP+ISSFGRIEENGLVVVRNL
Sbjct: 1   MEVSSNGQRSIYCLRHLNPKIFKCLATLLVL-LIFRVILFPTISSFGRIEENGLVVVRNL 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SPLYGSDFGIR+DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SPLYGSDFGIRVDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIF+FE FNS CNGFVRLGRY++ISNRTKPIELSKGSGRKWTIERDLEQL+
Sbjct: 121 ERLEPISFDKIFRFEEFNSRCNGFVRLGRYMNISNRTKPIELSKGSGRKWTIERDLEQLE 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEPFD+SEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVD+IEKE DKVISRIREVG
Sbjct: 181 EYSKEPFDQSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEVDKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           S+VRSKFD+D   V SE LLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM
Sbjct: 241 SKVRSKFDSDGTVVQSENLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI GMK P VVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 NRVGNILGMKMPAVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGV DLC TN+ WPSYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVVDLCDTNLSWPSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSC 447
           GP+KWISNMSD +LK ISYGSNDTSC
Sbjct: 421 GPRKWISNMSDTNLKNISYGSNDTSC 445

BLAST of Lsi02G010250 vs. ExPASy TrEMBL
Match: A0A5A7TPH5 (O-fucosyltransferase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G001110 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.1e-231
Identity = 401/446 (89.91%), Postives = 421/446 (94.39%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           MEVSSNG+RSIYCLR+ N +I KC+A LL++ LI R I FP+ISSFGRIEENGLVVVRNL
Sbjct: 1   MEVSSNGQRSIYCLRHLNPKIFKCLATLLVL-LIFRVILFPTISSFGRIEENGLVVVRNL 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SPLYGSDFGIR+DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SPLYGSDFGIRVDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIF+FE FNS CNGFVRLGRY++ISNRTKPIELSKGSGRKWTIERDLEQL+
Sbjct: 121 ERLEPISFDKIFRFEEFNSRCNGFVRLGRYMNISNRTKPIELSKGSGRKWTIERDLEQLE 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEPFD+SEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVD+IEKE DKVISRIREVG
Sbjct: 181 EYSKEPFDQSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEVDKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           S+VRSKFD+D   V SE LLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM
Sbjct: 241 SKVRSKFDSDGTVVQSENLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI GMK P VVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 NRVGNILGMKMPAVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGV DLC TN+ WPSYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVVDLCDTNLSWPSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSC 447
           GP+KWISNMSD +LK ISYGSNDTSC
Sbjct: 421 GPRKWISNMSDTNLKNISYGSNDTSC 445

BLAST of Lsi02G010250 vs. ExPASy TrEMBL
Match: A0A0A0LYP7 (O-fucosyltransferase family protein OS=Cucumis sativus OX=3659 GN=Csa_1G705610 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.4e-229
Identity = 399/447 (89.26%), Postives = 417/447 (93.29%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           MEVSSNG+RSIYCL   N RI KC+A LL + LIVR I FP+ISSFGRIEENGLVVVRNL
Sbjct: 1   MEVSSNGQRSIYCLHILNPRISKCLATLLFL-LIVRVILFPTISSFGRIEENGLVVVRNL 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SPLYGSDFGIR+DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SPLYGSDFGIRVDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPI FDKIFQFE FNS CNGFVRLGRY+DISN+TKPIEL KGSGRKWTIERDLEQL+
Sbjct: 121 ERLEPIFFDKIFQFEEFNSRCNGFVRLGRYMDISNQTKPIELLKGSGRKWTIERDLEQLE 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEPFD+SEVI IVGKNPFLWHDHWPVKDYAK+FECLVLVD+IEKE DKVISRIREVG
Sbjct: 181 EYSKEPFDQSEVITIVGKNPFLWHDHWPVKDYAKIFECLVLVDEIEKEVDKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           S+VRSKFD+DA  V SE  LQP+PYVAVHMRIEIDWMIHCKKLEQRS+INQICSSKEEIM
Sbjct: 241 SKVRSKFDSDATVVKSENSLQPMPYVAVHMRIEIDWMIHCKKLEQRSRINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI  MK PTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 NRVGNILEMKVPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKT V DLC TN+ WPSYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTDVVDLCDTNLSWPSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSCR 448
           GP+KWISNMSDISLK ISYGSNDTSCR
Sbjct: 421 GPRKWISNMSDISLKNISYGSNDTSCR 446

BLAST of Lsi02G010250 vs. ExPASy TrEMBL
Match: A0A6J1CNX9 (O-fucosyltransferase family protein OS=Momordica charantia OX=3673 GN=LOC111012751 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.7e-223
Identity = 388/446 (87.00%), Postives = 416/446 (93.27%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           MEVSS+GKRSIYCLRY NSRICKC+AILL + L+VRAI FPS+SSF RI ENGLV++RN 
Sbjct: 1   MEVSSSGKRSIYCLRYLNSRICKCVAILLAL-LVVRAIVFPSLSSFSRIGENGLVIIRNH 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           S    SD GIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SLWSSSDSGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIFQFE FNS CNG+VRLGRY+DISNRT+  EL KGSGRKWT+ERDLEQL+
Sbjct: 121 ERLEPISFDKIFQFEEFNSRCNGYVRLGRYMDISNRTRATELPKGSGRKWTVERDLEQLR 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKE FD  EVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVD+IEKEADKVI++IREVG
Sbjct: 181 EYSKELFDHHEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEADKVIAKIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           SEVRSKF +DA GV SEKLLQ VPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM
Sbjct: 241 SEVRSKFGSDATGVESEKLLQLVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           +RVGNI G KTP VVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 DRVGNILGTKTPVVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLR+DVFVGNSFSTFSSLVVL RTQK++KTGV DLC T+VRWPSYAYNILGD+K
Sbjct: 361 AIDYEVCLRSDVFVGNSFSTFSSLVVLERTQKMIKTGVEDLCSTDVRWPSYAYNILGDSK 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSC 447
           GP+KWI+NM+D+SL+KISYGSN+TSC
Sbjct: 421 GPRKWIANMADLSLQKISYGSNETSC 445

BLAST of Lsi02G010250 vs. ExPASy TrEMBL
Match: A0A6J1GT89 (O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC111456905 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 3.1e-214
Identity = 372/447 (83.22%), Postives = 408/447 (91.28%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           M+V SNGKRS+ CLRY NSRICKCIAILL++ LIVRAI FP  SSF RIEENGLVV+RN 
Sbjct: 1   MDVQSNGKRSVLCLRYLNSRICKCIAILLVL-LIVRAIVFP-FSSFDRIEENGLVVIRNR 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SP        R+DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SP-------FRLDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIF++E FNS CNGFVRLGRY+D+SNRTK IE+ KGSGRKWT+ERD EQLK
Sbjct: 121 ERLEPISFDKIFRYEEFNSRCNGFVRLGRYMDVSNRTKAIEIPKGSGRKWTVERDFEQLK 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEPFD++EV+RI GKNPFLWHDHWPVKDYAKVFECLVLVD+IEKEADKVISRIREVG
Sbjct: 181 EYSKEPFDQAEVVRIEGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEADKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           S+VRSK D+ A G  +  LLQPVPYVAVHMRIEIDWMIHCKKLE+RSK+NQICSSKEEI+
Sbjct: 241 SKVRSKLDSSATGAKARMLLQPVPYVAVHMRIEIDWMIHCKKLEKRSKMNQICSSKEEIV 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI GMKTPTVVYLAVADSLLNDSSI KGWK+G +P EK+KLGID+IYKKYPYL+QS
Sbjct: 301 NRVGNIIGMKTPTVVYLAVADSLLNDSSISKGWKDGFVPIEKRKLGIDEIYKKYPYLVQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVL RTQKL KTGV++LCGTNVRW SYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLERTQKLTKTGVSNLCGTNVRWRSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSCR 448
           G ++WI++MSD+SL+KISYGSNDTSCR
Sbjct: 421 GARRWIADMSDLSLEKISYGSNDTSCR 438

BLAST of Lsi02G010250 vs. NCBI nr
Match: XP_038894804.1 (O-fucosyltransferase 23 [Benincasa hispida])

HSP 1 Score: 847.8 bits (2189), Expect = 4.2e-242
Identity = 418/447 (93.51%), Postives = 431/447 (96.42%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           ME SSNGKRSIYCLRYFNSRICKCIAILL+IPLIVRAIFFPSISSFGRIEENGLVVVRNL
Sbjct: 1   MEASSNGKRSIYCLRYFNSRICKCIAILLVIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SP YGSDFGIRIDKFLEVPQIVWGLNNQKIAFARA LTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SPFYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARASLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIFQFE FNS CNGFVRLGRY+DISNRT P+ELSKGSGRKWTIERDLEQL 
Sbjct: 121 ERLEPISFDKIFQFEEFNSRCNGFVRLGRYMDISNRTNPMELSKGSGRKWTIERDLEQLN 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEP D+SEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVD+IEKEADKVISRIREVG
Sbjct: 181 EYSKEPLDQSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEADKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           SEVRSKF++DA  V SEK LQPVPY+AVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM
Sbjct: 241 SEVRSKFNSDATDVKSEKSLQPVPYIAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI+G+ TP V+YLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 NRVGNISGLTTPMVIYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGV DLCGTNVRWPSYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVEDLCGTNVRWPSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSCR 448
           GPQKWISNMSDISLKKISYGSNDTSC+
Sbjct: 421 GPQKWISNMSDISLKKISYGSNDTSCQ 447

BLAST of Lsi02G010250 vs. NCBI nr
Match: XP_008459099.1 (PREDICTED: uncharacterized protein LOC103498309 [Cucumis melo] >XP_016902351.1 PREDICTED: uncharacterized protein LOC103498309 [Cucumis melo] >KAA0043315.1 O-FucT domain-containing protein [Cucumis melo var. makuwa] >TYK29379.1 O-FucT domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 811.2 bits (2094), Expect = 4.4e-231
Identity = 401/446 (89.91%), Postives = 421/446 (94.39%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           MEVSSNG+RSIYCLR+ N +I KC+A LL++ LI R I FP+ISSFGRIEENGLVVVRNL
Sbjct: 1   MEVSSNGQRSIYCLRHLNPKIFKCLATLLVL-LIFRVILFPTISSFGRIEENGLVVVRNL 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SPLYGSDFGIR+DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SPLYGSDFGIRVDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIF+FE FNS CNGFVRLGRY++ISNRTKPIELSKGSGRKWTIERDLEQL+
Sbjct: 121 ERLEPISFDKIFRFEEFNSRCNGFVRLGRYMNISNRTKPIELSKGSGRKWTIERDLEQLE 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEPFD+SEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVD+IEKE DKVISRIREVG
Sbjct: 181 EYSKEPFDQSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEVDKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           S+VRSKFD+D   V SE LLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM
Sbjct: 241 SKVRSKFDSDGTVVQSENLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI GMK P VVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 NRVGNILGMKMPAVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGV DLC TN+ WPSYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVVDLCDTNLSWPSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSC 447
           GP+KWISNMSD +LK ISYGSNDTSC
Sbjct: 421 GPRKWISNMSDTNLKNISYGSNDTSC 445

BLAST of Lsi02G010250 vs. NCBI nr
Match: XP_011660318.1 (O-fucosyltransferase 23 [Cucumis sativus] >XP_011660319.1 O-fucosyltransferase 23 [Cucumis sativus] >XP_011660320.1 O-fucosyltransferase 23 [Cucumis sativus] >KGN66893.1 hypothetical protein Csa_006861 [Cucumis sativus])

HSP 1 Score: 803.9 bits (2075), Expect = 7.0e-229
Identity = 399/447 (89.26%), Postives = 417/447 (93.29%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           MEVSSNG+RSIYCL   N RI KC+A LL + LIVR I FP+ISSFGRIEENGLVVVRNL
Sbjct: 1   MEVSSNGQRSIYCLHILNPRISKCLATLLFL-LIVRVILFPTISSFGRIEENGLVVVRNL 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SPLYGSDFGIR+DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SPLYGSDFGIRVDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPI FDKIFQFE FNS CNGFVRLGRY+DISN+TKPIEL KGSGRKWTIERDLEQL+
Sbjct: 121 ERLEPIFFDKIFQFEEFNSRCNGFVRLGRYMDISNQTKPIELLKGSGRKWTIERDLEQLE 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEPFD+SEVI IVGKNPFLWHDHWPVKDYAK+FECLVLVD+IEKE DKVISRIREVG
Sbjct: 181 EYSKEPFDQSEVITIVGKNPFLWHDHWPVKDYAKIFECLVLVDEIEKEVDKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           S+VRSKFD+DA  V SE  LQP+PYVAVHMRIEIDWMIHCKKLEQRS+INQICSSKEEIM
Sbjct: 241 SKVRSKFDSDATVVKSENSLQPMPYVAVHMRIEIDWMIHCKKLEQRSRINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI  MK PTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 NRVGNILEMKVPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKT V DLC TN+ WPSYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTDVVDLCDTNLSWPSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSCR 448
           GP+KWISNMSDISLK ISYGSNDTSCR
Sbjct: 421 GPRKWISNMSDISLKNISYGSNDTSCR 446

BLAST of Lsi02G010250 vs. NCBI nr
Match: XP_022142703.1 (uncharacterized protein LOC111012751 [Momordica charantia])

HSP 1 Score: 783.5 bits (2022), Expect = 9.8e-223
Identity = 388/446 (87.00%), Postives = 416/446 (93.27%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           MEVSS+GKRSIYCLRY NSRICKC+AILL + L+VRAI FPS+SSF RI ENGLV++RN 
Sbjct: 1   MEVSSSGKRSIYCLRYLNSRICKCVAILLAL-LVVRAIVFPSLSSFSRIGENGLVIIRNH 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           S    SD GIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SLWSSSDSGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIFQFE FNS CNG+VRLGRY+DISNRT+  EL KGSGRKWT+ERDLEQL+
Sbjct: 121 ERLEPISFDKIFQFEEFNSRCNGYVRLGRYMDISNRTRATELPKGSGRKWTVERDLEQLR 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKE FD  EVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVD+IEKEADKVI++IREVG
Sbjct: 181 EYSKELFDHHEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEADKVIAKIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           SEVRSKF +DA GV SEKLLQ VPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM
Sbjct: 241 SEVRSKFGSDATGVESEKLLQLVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           +RVGNI G KTP VVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS
Sbjct: 301 DRVGNILGTKTPVVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLR+DVFVGNSFSTFSSLVVL RTQK++KTGV DLC T+VRWPSYAYNILGD+K
Sbjct: 361 AIDYEVCLRSDVFVGNSFSTFSSLVVLERTQKMIKTGVEDLCSTDVRWPSYAYNILGDSK 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSC 447
           GP+KWI+NM+D+SL+KISYGSN+TSC
Sbjct: 421 GPRKWIANMADLSLQKISYGSNETSC 445

BLAST of Lsi02G010250 vs. NCBI nr
Match: KAG6573448.1 (O-fucosyltransferase 23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 757.7 bits (1955), Expect = 5.7e-215
Identity = 376/447 (84.12%), Postives = 407/447 (91.05%), Query Frame = 0

Query: 1   MEVSSNGKRSIYCLRYFNSRICKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNL 60
           M+VSSNGKRS+ CLRY NSRICKCIAILL++ LIVRAI FP  SSF RIEENGLVV+RN 
Sbjct: 1   MDVSSNGKRSVLCLRYLNSRICKCIAILLVL-LIVRAIVFP-FSSFDRIEENGLVVIRNR 60

Query: 61  SPLYGSDFGIRIDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120
           SP +       +DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV
Sbjct: 61  SPFH-------LDKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEV 120

Query: 121 ERLEPISFDKIFQFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLK 180
           ERLEPISFDKIF++E FNS CNGFVRLGRY+D+SNRTK IE+ KGSGRKWT+ERD EQLK
Sbjct: 121 ERLEPISFDKIFRYEEFNSRCNGFVRLGRYMDVSNRTKAIEMPKGSGRKWTVERDFEQLK 180

Query: 181 EYSKEPFDKSEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG 240
           EYSKEPFD++EV+RI GKNPFLWHDHWPVKDYAKVFECLVLVD+IEKEADKVISRIREVG
Sbjct: 181 EYSKEPFDQAEVVRIEGKNPFLWHDHWPVKDYAKVFECLVLVDEIEKEADKVISRIREVG 240

Query: 241 SEVRSKFDADANGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIM 300
           SEVRSK D  A G  +  LLQPVPYVAVHMRIEIDWMIHCKKLEQRSK+NQICSSKEEI+
Sbjct: 241 SEVRSKLDTSATGAKARMLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKMNQICSSKEEIV 300

Query: 301 NRVGNITGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGIDKIYKKYPYLIQS 360
           NRVGNI GMKTPTVVYLAVADSLLNDSSI KGWK+G +P EKKKLGID IYKKYPYL+QS
Sbjct: 301 NRVGNIIGMKTPTVVYLAVADSLLNDSSISKGWKDGFVPLEKKKLGIDGIYKKYPYLVQS 360

Query: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLCGTNVRWPSYAYNILGDAK 420
           AIDYEVCLRADVFVGNSFSTFSSLVVL RTQKLMKTGV +LCGTNVRW SYAYNILGD+ 
Sbjct: 361 AIDYEVCLRADVFVGNSFSTFSSLVVLERTQKLMKTGVWNLCGTNVRWRSYAYNILGDSN 420

Query: 421 GPQKWISNMSDISLKKISYGSNDTSCR 448
           G ++WI++MSD+SL+KISYGSNDTSCR
Sbjct: 421 GARRWIADMSDLSLEKISYGSNDTSCR 438

BLAST of Lsi02G010250 vs. TAIR 10
Match: AT3G05320.1 (O-fucosyltransferase family protein )

HSP 1 Score: 543.5 bits (1399), Expect = 1.6e-154
Identity = 281/443 (63.43%), Postives = 347/443 (78.33%), Query Frame = 0

Query: 14  LRYFNSRI-CKCIAILLIIPLIVRAIFFPSISSFGRIEENGLVVVRNLSPLYGSDFGIRI 73
           LR F+  + CKC+ +L+ I L  RA+   S S    +  + L   R++S    S  GIR 
Sbjct: 10  LRIFSKSVACKCL-VLVGIALFYRALLL-SYSPRNALSNSLLFRDRHMSD-SSSTGGIRT 69

Query: 74  DKFLEVPQIVWGLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIF 133
           DKFLEVPQIVWGLNNQKIAFARACLTARM+NRTLLMPSLSASLFYKEV++L PI FDK+F
Sbjct: 70  DKFLEVPQIVWGLNNQKIAFARACLTARMMNRTLLMPSLSASLFYKEVDKLRPIPFDKVF 129

Query: 134 QFELFNSHCNGFVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLKEYSK-EPFDKSE 193
           QFE FNS C+GFVRL R+ D+ NR +  +L KGSGR+WT+ERDLE LK+ ++ E  D+ E
Sbjct: 130 QFERFNSLCSGFVRLSRFSDVKNRAQVFDLEKGSGRRWTVERDLEHLKQSARNESIDEFE 189

Query: 194 VIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVGSEVRSKFDADA 253
           VIR++GKNPFLWHDHWPV+DYAKVFEC+V+VD+I +EADKV+ +IRE G   R+K  +  
Sbjct: 190 VIRLIGKNPFLWHDHWPVEDYAKVFECMVVVDEISREADKVVMKIREAGEAERAKLAS-- 249

Query: 254 NGVISEKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIMNRVGNITGMKT 313
                 ++L PVP+VAVHMRIEIDWMIHCKKLEQR KI++ICSSK EI++RVGNI+G+KT
Sbjct: 250 ----KTEILGPVPFVAVHMRIEIDWMIHCKKLEQRKKISEICSSKREILDRVGNISGLKT 309

Query: 314 PTVVYLAVADSLL----NDSSILKGWKEGLLPFEKKKLGI-DKIYKKYPYLIQSAIDYEV 373
           PTV+YLAVAD+LL     DSS+L GW++GL+PFEKKKLG+ ++IY KY YLIQSAIDYEV
Sbjct: 310 PTVLYLAVADTLLEEKEEDSSVLSGWRDGLIPFEKKKLGVKEEIYGKYSYLIQSAIDYEV 369

Query: 374 CLRADVFVGNSFSTFSSLVVLGRTQKLMKTGVADLC--GTNVRWPSYAYNILGDAKG-PQ 433
           CLRADVFVGNSFSTFSSL+VL RTQK  + G    C  G N +W SYAYN+ G++ G P+
Sbjct: 370 CLRADVFVGNSFSTFSSLIVLERTQKARRLGFMSSCKDGGN-KWRSYAYNLAGESNGVPR 429

Query: 434 KWISNMSDISLKKISYGSNDTSC 447
           +W++NM+  SL+ ISYGSN  SC
Sbjct: 430 RWMTNMTHSSLQAISYGSNSVSC 442

BLAST of Lsi02G010250 vs. TAIR 10
Match: AT3G07900.1 (O-fucosyltransferase family protein )

HSP 1 Score: 50.8 bits (120), Expect = 3.2e-06
Identity = 76/328 (23.17%), Postives = 130/328 (39.63%), Query Frame = 0

Query: 84  GLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIFQFELFNSHCNG 143
           GLN QKI    A + AR+L   L++P L  +L + +        F  IF  E F S    
Sbjct: 199 GLNQQKIQIVDAVVIARILGAVLVVPILQINLIWGDES-----EFSDIFDLEQFKSVLAN 258

Query: 144 FVRLGRYIDISN-RTKPIELSKGSGRKWTIERDLEQLKEYSKEPFDKSEVIRIVGKNPFL 203
            V++   +  S   T+P E   GS          + ++ +  + F++  V+ +   +  L
Sbjct: 259 DVKIVSLLPASKVMTRPSE--DGS---MPFNASPQWIRSHYPKRFNREGVLLLRRLDSRL 318

Query: 204 WHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVGSEVRSKFDADANGVISEKLLQP 263
             D   +    +   C     K+  EA K   R+ E+G++            ++E++   
Sbjct: 319 SKD---LPSDLQKLRC-----KVAFEALKFSPRVMEMGTK------------LAERMRSK 378

Query: 264 VPYVAVHMRIEIDWMIHCKKLE------------QRSKINQICSSKEEIMNRVGNITG-- 323
            PY+A+H+R+E D  +    L             +R K  ++ ++K  + +    + G  
Sbjct: 379 GPYIALHLRMEKDVWVRTGCLSGLSSKYDEIVNIERIKRPELLTAKSSMTSNERKLAGLC 438

Query: 324 -MKTPTVVYLAVADSLLNDSSIL------KGWKEGLLPFEKKKLGIDKIYKKY------- 377
            +    V  L  A     D+ I        G KE L P   +      +Y KY       
Sbjct: 439 PLNAKEVTRLLRALGAPRDARIYWAGGEPLGGKEALKPLTSE---FPHLYNKYDIALPLE 493

BLAST of Lsi02G010250 vs. TAIR 10
Match: AT1G62330.1 (O-fucosyltransferase family protein )

HSP 1 Score: 50.1 bits (118), Expect = 5.5e-06
Identity = 57/219 (26.03%), Postives = 99/219 (45.21%), Query Frame = 0

Query: 84  GLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIFQFELFNSHCNG 143
           G+N Q++A     + ARMLN TL++P    S  + +  +     F  I+Q E F  + + 
Sbjct: 232 GINQQRVAVCNIVVVARMLNATLVIPKFMFSDVWTDASQ-----FGDIYQVEHFIKYLSP 291

Query: 144 FVRLGRYIDISNRTKPIELSKGSGRKWTIERDLEQLKE-----YSKEPFD---KSEVIRI 203
            +R+ + +    ++  +E + GS     +  D++ +KE     Y K       K+ V+  
Sbjct: 292 DIRIVKKLPKELQSLDLE-AIGS-----VVTDIDVMKEAKPGFYMKHILPLLLKNRVVHF 351

Query: 204 VGKNPFLWHDHWPVKDYAKVFEC----LVLVDKIEKEADKVISRIREVGSE--------V 263
           +G    L  D  P +       C    L  V KI++    ++ R+R+ GS         V
Sbjct: 352 LGFGNRLAFDPIPFELQRLRCRCNFHALNFVPKIQETGAILVRRLRDSGSHLAPVDPYLV 411

Query: 264 RSKFDADANGVISEK---LLQPVPYVAVHMRIEIDWMIH 280
             KF   A+ ++ +K   L +   Y+AVH+R EID + H
Sbjct: 412 GPKF---ASFILDKKAGPLHKASKYLAVHLRFEIDMVAH 436

BLAST of Lsi02G010250 vs. TAIR 10
Match: AT3G26370.1 (O-fucosyltransferase family protein )

HSP 1 Score: 49.7 bits (117), Expect = 7.2e-06
Identity = 52/205 (25.37%), Postives = 92/205 (44.88%), Query Frame = 0

Query: 84  GLNNQKIAFARACLTARMLNRTLLMPSLSASLFYKEVERLEPISFDKIFQFELFNSHCNG 143
           GLN Q+IA   A   A+++N TL++P L     +K+  +     F+ IF  + F  +   
Sbjct: 169 GLNQQRIAICNAVAVAKIMNATLILPVLKQDQIWKDTTK-----FEDIFDVDHFIDYLKD 228

Query: 144 FVRLGRYIDISN-RTKPIELSKGSGRKWTIERDLEQLKEYSKEPFDKSEVI------RIV 203
            VR+ R  DI +  T   EL        +I R ++ + +Y+   F    V+      +I+
Sbjct: 229 DVRIVR--DIPDWFTDKAELFS------SIRRTVKNIPKYAAAQFYIDNVLPRIKEKKIM 288

Query: 204 GKNPF---LWHDHWPVKDYAKV-----FECLVLVDKIEKEADKVISRIREVGSEVRSKFD 263
              PF   L +D+ P ++  ++     +  L  + +IE+ AD ++SR+R           
Sbjct: 289 ALKPFVDRLGYDNVP-QEINRLRCRVNYHALKFLPEIEQMADSLVSRMR----------- 340

Query: 264 ADANGVISEKLLQPVPYVAVHMRIE 274
                    +   P PY+A+H+R E
Sbjct: 349 --------NRTGNPNPYMALHLRFE 340

BLAST of Lsi02G010250 vs. TAIR 10
Match: AT1G04910.1 (O-fucosyltransferase family protein )

HSP 1 Score: 44.3 bits (103), Expect = 3.0e-04
Identity = 72/311 (23.15%), Postives = 124/311 (39.87%), Query Frame = 0

Query: 84  GLNNQKIAFARACLTARMLNRTLLMPSLSASLFYK---------EVER-LEPISFDKIFQ 143
           GLN Q+ A   A L AR++N TL++P L A+ F+          +VE  +E + +D    
Sbjct: 99  GLNQQRSAICNAVLAARIMNATLVLPELDANSFWHDDSGFQGIYDVEHFIETLKYDVKIV 158

Query: 144 FELFNSHCNGFVRLGRYIDI-SNRTKPIELSKGSGRKWTIERDLEQLKEYSK---EPFDK 203
            ++ + H NG  +  +   I   R  PIE        W +   L+ ++E+S     PF  
Sbjct: 159 GKIPDVHKNGKTKKIKAFQIRPPRDAPIE--------WYLTTALKAMREHSAIYLTPFSH 218

Query: 204 SEVIRIVGKNPFLWHDHWPVKDYAKVFECLVLVDKIEKEADKVISRIREVG--SEVRSKF 263
                I   NP        V  +A  F+       I K ++ ++ ++R  G    +  +F
Sbjct: 219 RLAEEI--DNPEYQRLRCRVNYHALRFK-----PHIMKLSESIVDKLRSQGHFMSIHLRF 278

Query: 264 DADANGVIS-EKLLQPVPYVAVHMRIEIDWMIHCKKLEQRSKINQICSSKEEIMNRVGNI 323
           + D         +  P     +    + ++        +R  I + C    E +  +   
Sbjct: 279 EMDMLAFAGCFDIFNPEEQKILRKYRKENFADKRLIYNERRAIGK-CPLTPEEVGLILRA 338

Query: 324 TGMKTPTVVYLAVADSLLNDSSILKGWKEGLLPFEKKKLGID---KIYKKYPYLIQSAID 375
                 T +YLA A  L      +K ++  L P       +D   ++      LI SA+D
Sbjct: 339 MRFDNSTRIYLA-AGELFGGEQFMKPFRT-LFPRLDNHSSVDPSEELSATSQGLIGSAVD 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9MA872.2e-15363.43O-fucosyltransferase 23 OS=Arabidopsis thaliana OX=3702 GN=OFUT23 PE=2 SV=1[more]
F4HYR47.8e-0526.03O-fucosyltransferase 15 OS=Arabidopsis thaliana OX=3702 GN=OFUT15 PE=2 SV=1[more]
Q9LIN91.0e-0425.37Protein PECTIC ARABINOGALACTAN SYNTHESIS-RELATED OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A1S4E2962.1e-23189.91O-fucosyltransferase family protein OS=Cucumis melo OX=3656 GN=LOC103498309 PE=3... [more]
A0A5A7TPH52.1e-23189.91O-fucosyltransferase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A0A0LYP73.4e-22989.26O-fucosyltransferase family protein OS=Cucumis sativus OX=3659 GN=Csa_1G705610 P... [more]
A0A6J1CNX94.7e-22387.00O-fucosyltransferase family protein OS=Momordica charantia OX=3673 GN=LOC1110127... [more]
A0A6J1GT893.1e-21483.22O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC11145690... [more]
Match NameE-valueIdentityDescription
XP_038894804.14.2e-24293.51O-fucosyltransferase 23 [Benincasa hispida][more]
XP_008459099.14.4e-23189.91PREDICTED: uncharacterized protein LOC103498309 [Cucumis melo] >XP_016902351.1 P... [more]
XP_011660318.17.0e-22989.26O-fucosyltransferase 23 [Cucumis sativus] >XP_011660319.1 O-fucosyltransferase 2... [more]
XP_022142703.19.8e-22387.00uncharacterized protein LOC111012751 [Momordica charantia][more]
KAG6573448.15.7e-21584.12O-fucosyltransferase 23, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT3G05320.11.6e-15463.43O-fucosyltransferase family protein [more]
AT3G07900.13.2e-0623.17O-fucosyltransferase family protein [more]
AT1G62330.15.5e-0626.03O-fucosyltransferase family protein [more]
AT3G26370.17.2e-0625.37O-fucosyltransferase family protein [more]
AT1G04910.13.0e-0423.15O-fucosyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.11350coord: 245..398
e-value: 3.5E-27
score: 97.1
NoneNo IPR availablePANTHERPTHR37220:SF1O-FUCOSYLTRANSFERASE 23coord: 1..446
NoneNo IPR availableCDDcd11296O-FucT_likecoord: 75..390
e-value: 7.2747E-24
score: 96.7175
IPR019378GDP-fucose protein O-fucosyltransferasePFAMPF10250O-FucTcoord: 84..388
e-value: 6.7E-28
score: 98.4
IPR044982O-FUCOSYLTRANSFERASE1-likePANTHERPTHR37220O-FUCOSYLTRANSFERASE 23coord: 1..446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G010250.1Lsi02G010250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006004 fucose metabolic process
biological_process GO:0009875 pollen-pistil interaction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016757 glycosyltransferase activity