Lsi02G009700 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G009700 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 7.3e-98
Identity = 202/441 (45.80%), Postives = 267/441 (60.54%), Query Frame = 0

Query: 4   LAFIIVSILTFGI-WESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 63
           L F   S L   I    SAN      FLQCLSL      + +  +I+TP ++SFS +L  
Sbjct: 11  LIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQL-NDSNIVSKVIHTPNDTSFSSVLAS 70

Query: 64  YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 123
            I+N RFS    PKP+LI+TP+QP  VQ+ V C +  G  IR RSGGHDYEGLSYV++ P
Sbjct: 71  SIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKP 130

Query: 124 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 183
                                              I +K++TLAFPAG CPTVGVGGH S
Sbjct: 131 FVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFS 190

Query: 184 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 243
           GGG+G LLRK+GLAADHVIDA +VDA G++L+R+ MGED FWAIRGGGG SF +VL+WKI
Sbjct: 191 GGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKI 250

Query: 244 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 303
            L+ VP TVTVF V K  +Q+ LK+IH+WQ+VA K+ D+L    F R        + +  
Sbjct: 251 GLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDL----FIRV-------MLQRY 310

Query: 304 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEE 363
              V ASF  L+LG    L+ ++ K FPELGL ++DC E +WIES++  A     E  EE
Sbjct: 311 KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA-----ELGEE 370

Query: 364 AL--LNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 407
            +  L + T  S   A K KSD+V+EP+ +T +  LW+ L+    E A ++  P+GGKM+
Sbjct: 371 PINVLTKRTRASL--AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMS 430

BLAST of Lsi02G009700 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 9.6e-98
Identity = 201/441 (45.58%), Postives = 271/441 (61.45%), Query Frame = 0

Query: 5   AFIIVSILTFGIWESSANYQKPIEFLQCLSLHS-PKGDDSIFNLIYTPTNSSFSHILNFY 64
           A I+V      IW+SS++      F QCL+ +S PK    I   I+   N S+S +L   
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPK--HPISPAIFFSGNGSYSSVLQAN 63

Query: 65  IRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVS---- 124
           IRNLRF+T +TPKP LII      HVQA + C K H  Q++IRSGGHDY+GLSYV+    
Sbjct: 64  IRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGK 123

Query: 125 -------------NVPIA--------------------EKSKTLAFPAGGCPTVGVGGHL 184
                        +V +A                    EKSKTLA+PAG CPTVGVGGH+
Sbjct: 124 PFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHI 183

Query: 185 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 244
           SGGG+G ++RKYGL  D+ IDA +VD NGK+LDRK MGEDL+WAI GGGGGS+G+VLA+K
Sbjct: 184 SGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYK 243

Query: 245 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRP-NNLKKGGIRR 304
           I L+ VP  VTVF +++ L+QN   +IH+WQ VA KL D L    F R   ++  G +  
Sbjct: 244 INLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDEL----FIRTVIDVVNGTV-- 303

Query: 305 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 364
           +  +TV  +F ++FLG    L+SI+ + FPELGL++ DC E +WI+S+L       G S 
Sbjct: 304 SSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS- 363

Query: 365 EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 407
           E  LL R+   +++   K KSDYV+EPIS TGL+ +WK  +   +E  ++   PYGG+M 
Sbjct: 364 ETLLLQRNQPVNYL---KRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMG 423

BLAST of Lsi02G009700 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.1e-93
Identity = 191/435 (43.91%), Postives = 264/435 (60.69%), Query Frame = 0

Query: 8   IVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSS-FSHILNFYIRN 67
           +V++L   +  SS+  Q+  +F++CL  +S        +      N++ F   L    +N
Sbjct: 14  LVTLLLISVPLSSSTLQQ--DFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 73

Query: 68  LRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSN------ 127
           LR+ T + PKP+ I  PL   HVQA V+C K     +R+RSGGHDYEGLS+V+       
Sbjct: 74  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 133

Query: 128 -------------------------------VPIAEKSKTLAFPAGGCPTVGVGGHLSGG 187
                                            I EKS+T  FPAG C ++G+GGHL GG
Sbjct: 134 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 193

Query: 188 GHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIKL 247
            +G ++RK+GL AD+V+DA +VDANG++LDR +MGED+FWAIRGGGGGSFG++LAWKIKL
Sbjct: 194 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 253

Query: 248 LYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRTR 307
           + VP TVTVFTV K L+Q+  K++++W+ +A KLDD+L    F R          +   R
Sbjct: 254 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDL----FIRVIISPASKTTKPGNR 313

Query: 308 TVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEEAL 367
           T++ S+ + FLG +N L+ +MQKSFPELGL K+DC E +WI+S++ +AGF N  + E  L
Sbjct: 314 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL 373

Query: 368 LNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEISE 405
             +S    F    K KSD+VKEPI   GL+GLW+  R    +S   +  PYGG M+ ISE
Sbjct: 374 AGKSL---FKNHFKAKSDFVKEPIPVEGLEGLWE--RFLEEDSPLTIWNPYGGMMSRISE 433

BLAST of Lsi02G009700 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.4e-93
Identity = 197/459 (42.92%), Postives = 277/459 (60.35%), Query Frame = 0

Query: 1   MNFLAFIIVSILTFGIWES---SANYQKPIEFLQCLSLHSPKGDDSIF-NLIYTPTNSSF 60
           M F +F+  +IL F +  S   SA+     +FL+CLS      D+++   +I+T  +SSF
Sbjct: 1   MEFSSFLF-TILLFSLNISPLVSAHGSNHEDFLKCLSYR--MNDNTVEPKVIHTSKDSSF 60

Query: 61  SHILNFYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLS 120
             IL+  I+N RFS + TPKP+ IITP++   VQ ++ C ++HG  +R RS GH YEGLS
Sbjct: 61  FSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLS 120

Query: 121 YVS-NVP-----------------------------------IAEKSKTLAFPAGGCPTV 180
           Y++ N P                                   I + +K+LAFPAG  PTV
Sbjct: 121 YIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTV 180

Query: 181 GVGGHLSGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFG 240
           GVGG  SGGG+G LLRKYGLAAD++IDAL+VDA+G++LDR++MGED FWAIRGGGG SFG
Sbjct: 181 GVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFG 240

Query: 241 IVLAWKIKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKK 300
           ++L+WK+KL+ VP T+TVF V K   +  +++I +WQY A K+ D+L    F R      
Sbjct: 241 VILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDL----FIRTT---- 300

Query: 301 GGIRRTRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFT 360
             + R+    V+A F  L++G  N L+++M++ FPELGL KE C E +WIES+L  A F 
Sbjct: 301 --LERSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFP 360

Query: 361 NGESLEEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPY 419
            GESL        T+ SF G    K D+V+EPI E  ++ +W+ L         I+L P+
Sbjct: 361 KGESLGVLTNRERTSLSFKG----KDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPF 420

BLAST of Lsi02G009700 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.4e-93
Identity = 203/461 (44.03%), Postives = 275/461 (59.65%), Query Frame = 0

Query: 3   FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFN--LIYTPTNSSFSHIL 62
           F+  II   L+F I  S AN ++   FL+C S H P   +++ N  L+YT  +  +  IL
Sbjct: 10  FVCKIIFFFLSFHIQISIANPRE--NFLKCFSKHIP---NNVANPKLVYTQHDQLYMSIL 69

Query: 63  NFYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSN 122
           N  I+NLRF + TTPKPL+I+TP    H+QA ++C K  G QIR RSGGHD EG+SY+S 
Sbjct: 70  NSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQ 129

Query: 123 VP-----------------------------------IAEKSKTLAFPAGGCPTVGVGGH 182
           VP                                   I EK++ L+FP G CPTVGVGGH
Sbjct: 130 VPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGH 189

Query: 183 LSGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAW 242
            SGGG+G L+R YGLAAD++IDA LV+ +GK+LDRKSMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 190 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 249

Query: 243 KIKLLYVPPTVTVFTVNKKLD-QNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKG-GI 302
           KIKL+ VP   T+F+V K ++    +KL ++WQ +A K D +L     F   N+    G 
Sbjct: 250 KIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGK 309

Query: 303 RRTRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAG---FT 362
            +T   TV+  F S+F G  + L+ +M KSFPELG+ K DC E +WI++ +  +G   F 
Sbjct: 310 NKT---TVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFN 369

Query: 363 NGESLEEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPY 420
                +E LL+RS       A  +K DYVK+PI ET +  + + L   ++ +   VL PY
Sbjct: 370 TANFKKEILLDRSAGKK--TAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPY 429

BLAST of Lsi02G009700 vs. ExPASy TrEMBL
Match: A0A6J1EH36 (cannabidiolic acid synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111434053 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 8.8e-163
Identity = 310/450 (68.89%), Postives = 338/450 (75.11%), Query Frame = 0

Query: 2   NFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILN 61
           +FLA    SILT   WESSAN  + + FL+CLSLHSP GDDSI NLIY  TNSSFS ILN
Sbjct: 6   SFLALTFASILTLETWESSAN--EHLHFLRCLSLHSPNGDDSISNLIYPQTNSSFSSILN 65

Query: 62  FYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNV 121
           F +RNLRFST TTPKPLLI+TP QPFHVQALVICCK HGFQIRIRSGGHDYEGLSYVS+V
Sbjct: 66  FSLRNLRFSTPTTPKPLLIVTPSQPFHVQALVICCKAHGFQIRIRSGGHDYEGLSYVSDV 125

Query: 122 P-----------------------------------IAEKSKTLAFPAGGCPTVGVGGHL 181
           P                                   IAEKS+TLAFPAGGCPTVGVGGH 
Sbjct: 126 PFVLVDLGEFRSIAIDIENNTAWVQSGATLGELHYRIAEKSQTLAFPAGGCPTVGVGGHF 185

Query: 182 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 241
           SGGGHGGL+RKYGLAADHVIDA+LVDANG +LDRK MGEDLFWAIRGGGGGSFGIVL+WK
Sbjct: 186 SGGGHGGLVRKYGLAADHVIDAILVDANGMVLDRKLMGEDLFWAIRGGGGGSFGIVLSWK 245

Query: 242 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLG-GKDFFRPNNLKKGGIRR 301
           I+                           WQYVASK+D+NL  G       N ++    R
Sbjct: 246 IR---------------------------WQYVASKVDENLFIGITLSTGPNSEERQRTR 305

Query: 302 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 361
           TRTRTVNASFFSLFLG+AN+L+SIMQ+SFPEL L K+DCIE +WIES L LAGFTN ESL
Sbjct: 306 TRTRTVNASFFSLFLGKANDLLSIMQQSFPELELAKQDCIEKSWIESNLILAGFTNRESL 365

Query: 362 EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 416
           EEALLNRST PSFIGA KVKSDYVKEPIS+TGL G+W MLRTT+I+SASIVLLPYGGKMN
Sbjct: 366 EEALLNRSTTPSFIGAYKVKSDYVKEPISQTGLPGVWHMLRTTDIDSASIVLLPYGGKMN 425

BLAST of Lsi02G009700 vs. ExPASy TrEMBL
Match: A0A5J4Z8V5 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_018019 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 5.4e-128
Identity = 248/448 (55.36%), Postives = 313/448 (69.87%), Query Frame = 0

Query: 2   NFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILN 61
           + L+ + V +L    WE+SA+  +  +FLQCLSLHS +   SI N+IYTP N+S+  IL 
Sbjct: 7   SLLSLLFVFLLPIS-WEASAHTHE--DFLQCLSLHS-QHSASISNVIYTPNNASYLPILE 66

Query: 62  FYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNV 121
           F I+NLRFS+ATTPKPL+I+TPL    +QA + C + HG QIR+RSGGHDYEGLSYVS++
Sbjct: 67  FSIQNLRFSSATTPKPLVIVTPLHESQIQATIYCSRNHGMQIRVRSGGHDYEGLSYVSDI 126

Query: 122 P-----------------------------------IAEKSKTLAFPAGGCPTVGVGGHL 181
           P                                   IAEKSKTLAFPAG CPT+GVGGH 
Sbjct: 127 PFIIVDLINLRSITVDAENSTAWVQAGATVGELYYRIAEKSKTLAFPAGVCPTIGVGGHF 186

Query: 182 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 241
           SGGG+G LLRKYGLAAD++IDA L+D NG++LDR+SMGEDLFWAIRGGGG SFG++LAWK
Sbjct: 187 SGGGYGTLLRKYGLAADNIIDARLMDVNGRILDRESMGEDLFWAIRGGGGASFGVILAWK 246

Query: 242 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRT 301
           IKL  VP TVTVFTV + L+QN  K++HQWQYVA K  ++L  +   R  N       + 
Sbjct: 247 IKLAPVPSTVTVFTVRRTLEQNATKIVHQWQYVAHKFPEDLFIRILIRSLNSS-----QD 306

Query: 302 RTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE 361
             RT+ ASF SLFLG  ++LIS+MQ+SFPELGL+KEDCIE +WIESIL  AGF +G S  
Sbjct: 307 EKRTIQASFNSLFLGGVDKLISLMQESFPELGLVKEDCIEMSWIESILYFAGFPSGASF- 366

Query: 362 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 415
           + LL+R+  P      K KSDYVKEPISE+GL+G+WK     ++E+A ++L PYGG+M+E
Sbjct: 367 DVLLDRT--PLARSYFKAKSDYVKEPISESGLEGIWKQFYEEDVEAAEMILSPYGGRMDE 426

BLAST of Lsi02G009700 vs. ExPASy TrEMBL
Match: A0A6J1DG91 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020167 PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 1.5e-125
Identity = 243/434 (55.99%), Postives = 294/434 (67.74%), Query Frame = 0

Query: 16  IWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFYIRNLRFSTATTP 75
           ++ S+A+     +FL CLSLHSP G  SI N+IYTPTN S+S +LNF   NLRF+T+ TP
Sbjct: 15  LFVSAASAHNHEDFLHCLSLHSPNG-SSISNIIYTPTNPSYSSVLNFSTHNLRFTTSETP 74

Query: 76  KPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP------------- 135
           KP +I+TPLQ   VQA ++C K +GFQIR RSGGHDYEGLSYVS+V              
Sbjct: 75  KPQVIVTPLQVSQVQASIVCAKNNGFQIRTRSGGHDYEGLSYVSDVSFIVVDLINLRSIN 134

Query: 136 ----------------------IAEKSKTLAFPAGGCPTVGVGGHLSGGGHGGLLRKYGL 195
                                 IAEKSKTL FPAG CPTVGVGGH SGGG+G +LRK+GL
Sbjct: 135 IDVEKKFAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGL 194

Query: 196 AADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIKLLYVPPTVTVFT 255
           AAD+V+DA  +D NGKL DRKSMGED+FWA+RGGGG SFGIVLAWKIKLL VPP VTVFT
Sbjct: 195 AADNVVDAYFIDVNGKLHDRKSMGEDVFWAVRGGGGASFGIVLAWKIKLLPVPPVVTVFT 254

Query: 256 VNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRTRTVNASFFSLFL 315
           V + L+QN   L+H+WQYVA K DD L  +   R  N      +    RT+ A+F SLFL
Sbjct: 255 VTRNLEQNATDLVHRWQYVADKQDDKLFIRIILRGVNSTD---QYGEKRTIEAAFNSLFL 314

Query: 316 GQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEEALLNRSTNPSFIG 375
           G+  +L+SIM K+FP LGL KEDCI+ +WIESIL  AGF  GE LE  L  R   P    
Sbjct: 315 GKVEKLLSIMHKTFPGLGLAKEDCIQMSWIESILYFAGFPRGERLEVLLDRRPLVPKRF- 374

Query: 376 ANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEISESEIPYPHRAGY 415
             K KSDYV EPI + GL+G+WK+    + E+A ++L PYGGKMNEISESEIP+PHRAG 
Sbjct: 375 -FKAKSDYVNEPIPKPGLEGIWKLFNEEDAEAAEMILSPYGGKMNEISESEIPFPHRAGN 434

BLAST of Lsi02G009700 vs. ExPASy TrEMBL
Match: A0A2I4E4X9 (berberine bridge enzyme-like 18 OS=Juglans regia OX=51240 GN=LOC108986335 PE=3 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 5.6e-125
Identity = 240/454 (52.86%), Postives = 313/454 (68.94%), Query Frame = 0

Query: 4   LAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFY 63
           + F+I  + +F +W +SA+  +  +FLQCL+LH+     SI  +IYTP NSS+S +L F 
Sbjct: 10  IPFVIAPLFSF-LWATSAHTHE--DFLQCLALHAGNSTSSISQVIYTPFNSSYSSVLKFS 69

Query: 64  IRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP- 123
           IRN RFST  TPKPL+IITPL    +QA + C + HG QIR+RSGGHD+EGLSYVS VP 
Sbjct: 70  IRNPRFSTPATPKPLVIITPLHVSQIQATIKCSQKHGMQIRVRSGGHDFEGLSYVSYVPF 129

Query: 124 ----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLSG 183
                                             IAEKS+   FPAG CPTVGVGGH SG
Sbjct: 130 VIIDLINLRSINVDVENGTAWVEAGATIGEVYYRIAEKSRNFGFPAGVCPTVGVGGHFSG 189

Query: 184 GGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIK 243
           GG+G +LRKYGLAAD+++DA ++D  G++LDR+SMGEDLFWAIRGGGG SFG+++AWKIK
Sbjct: 190 GGYGTMLRKYGLAADNIVDAQMIDVKGRILDRESMGEDLFWAIRGGGGASFGVIVAWKIK 249

Query: 244 LLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFR-----PNNLKKGGI 303
           L++VP TVTVFTVN+ L+QN  KL+H+WQYVA KLD++L    F R      N+ ++GG 
Sbjct: 250 LVHVPSTVTVFTVNRNLEQNATKLVHRWQYVADKLDEDL----FIRVILSAANSSQEGG- 309

Query: 304 RRTRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGE 363
                RT+ ASF SL+LG  + L+S+MQ+SFPELGL++EDC E +WIES L LAGF +GE
Sbjct: 310 -----RTIQASFKSLYLGGMDNLLSLMQESFPELGLVREDCTEMSWIESTLYLAGFPSGE 369

Query: 364 SLEEALLNRS--TNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYG 416
           SL + LL+R+  T P+F    KVKSDYV EPI E GL+G+W+       E A ++L+PYG
Sbjct: 370 SL-DVLLSRTLLTRPTFF---KVKSDYVMEPIPEVGLEGIWERFYHKEAEEAELLLIPYG 429

BLAST of Lsi02G009700 vs. ExPASy TrEMBL
Match: A0A2I4FDC6 (berberine bridge enzyme-like 18 OS=Juglans regia OX=51240 GN=LOC108997731 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 5.6e-125
Identity = 235/445 (52.81%), Postives = 305/445 (68.54%), Query Frame = 0

Query: 8   IVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNFYIRNL 67
           ++++L+  +W +SA+  +  +FLQCL+LH+     SI  +IYTP NSS+S +L F IRN 
Sbjct: 13  VITLLSSFLWATSAHTHE--DFLQCLTLHAGNSTSSISQVIYTPFNSSYSSVLEFSIRNP 72

Query: 68  RFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP----- 127
           RFST  TPKPL+IITPL    +QA + C + HG QIR+RSGGHDYEGLSYVS VP     
Sbjct: 73  RFSTPATPKPLVIITPLHVSQIQATIKCSQKHGMQIRVRSGGHDYEGLSYVSYVPFVIID 132

Query: 128 ------------------------------IAEKSKTLAFPAGGCPTVGVGGHLSGGGHG 187
                                         IAEKS+   FPAG CPTVGVGGH SGGG+G
Sbjct: 133 LINLRSINVDVENATAWVEAGAIIGEVYYRIAEKSRNFGFPAGVCPTVGVGGHFSGGGYG 192

Query: 188 GLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIKLLYV 247
            +LRKYGLAAD+++DA ++D  G++LDR SMGEDLFWAIRGGGG SFG+++AWKIKL++V
Sbjct: 193 TMLRKYGLAADNIVDAQMIDVKGRILDRDSMGEDLFWAIRGGGGASFGVIVAWKIKLVHV 252

Query: 248 PPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRTRTVN 307
           P TVTVFTVN+ L+QN  KL+H+WQYVA KLD++L  +      N  + G      RT+ 
Sbjct: 253 PSTVTVFTVNRNLEQNATKLVHRWQYVADKLDEDLFIRVILSAANSSQEG-----RRTIQ 312

Query: 308 ASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEEALLNR 367
           ASF SL+LG  + L+S+MQ+SFPELGL++EDC E +WIES L  AGF +GESL + LL+R
Sbjct: 313 ASFNSLYLGGIDNLLSLMQESFPELGLVREDCTEMSWIESTLYFAGFPSGESL-DVLLSR 372

Query: 368 S--TNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEISES 416
           +  T P+F    K KSDYV EPI E GL+G+W+       E A ++L PYGG+M+EISES
Sbjct: 373 TPLTRPTFF---KAKSDYVMEPIPEVGLEGIWERFYQKEAEEAELILSPYGGRMSEISES 432

BLAST of Lsi02G009700 vs. NCBI nr
Match: XP_038894066.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 705.7 bits (1820), Expect = 2.4e-199
Identity = 367/454 (80.84%), Postives = 385/454 (84.80%), Query Frame = 0

Query: 2   NFLAFIIV-SILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHIL 61
           NFLA  IV S+LTFGIWESSAN   P EFLQCLSLHSPKG DSIFNLIYTPTNSSFSHIL
Sbjct: 6   NFLASTIVFSVLTFGIWESSAN-PYPFEFLQCLSLHSPKGADSIFNLIYTPTNSSFSHIL 65

Query: 62  NFYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSN 121
           NF IRNLRFSTATTPKPLLIITPL P HVQALVICCKVHGFQIRIRSGGHDYEGLSYVS+
Sbjct: 66  NFSIRNLRFSTATTPKPLLIITPLHPSHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSD 125

Query: 122 VP-----------------------------------IAEKSKTLAFPAGGCPTVGVGGH 181
           VP                                   IA+KS+TLAFPAGGCPTVG+GGH
Sbjct: 126 VPFMLVDLGDYRSIAIDTQNNTAWVESGATLGELHYRIAKKSQTLAFPAGGCPTVGIGGH 185

Query: 182 LSGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAW 241
           LSGGGHGGLLRKYGLAADHV+DALLVDANGKLLDRKSMGEDLFWAIR GGGGSFGIVLAW
Sbjct: 186 LSGGGHGGLLRKYGLAADHVVDALLVDANGKLLDRKSMGEDLFWAIRAGGGGSFGIVLAW 245

Query: 242 KIKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNL--GGKDFFRPNNLKKGGI 301
           KIKL+YVPPTVTVFTV+KK+DQNTLKLIHQWQYVASKLD+NL  G      PNN ++GG 
Sbjct: 246 KIKLVYVPPTVTVFTVSKKMDQNTLKLIHQWQYVASKLDENLFIGITLIIGPNNSRQGGR 305

Query: 302 R-RTRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNG 361
           R RTRTRTVNASFFSLFLG+ANEL+SIMQKSFPELGLLKEDCIENNWIES L LAGFTNG
Sbjct: 306 RTRTRTRTVNASFFSLFLGKANELLSIMQKSFPELGLLKEDCIENNWIESFLILAGFTNG 365

Query: 362 ESLEEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGG 417
           ESLEEALLNRST PS+IGA KVKSDYVKEPISE GL G+W+MLRTTNIESASIVLLPYGG
Sbjct: 366 ESLEEALLNRSTTPSYIGAYKVKSDYVKEPISEIGLNGMWQMLRTTNIESASIVLLPYGG 425

BLAST of Lsi02G009700 vs. NCBI nr
Match: XP_023519292.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.3 bits (1653), Expect = 5.7e-180
Identity = 330/450 (73.33%), Postives = 364/450 (80.89%), Query Frame = 0

Query: 2   NFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILN 61
           +FLAF   SILT G WESSAN  + + FL+CLSLHSP GDDSI NLIY  TNSSFS ILN
Sbjct: 6   SFLAFTFASILTLGTWESSAN--EDLHFLRCLSLHSPNGDDSISNLIYPQTNSSFSSILN 65

Query: 62  FYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNV 121
           F +RNLRFST TTPKPLLI TP QP HVQALVICCK HGFQIRIRSGGHDYEGLSYVS+V
Sbjct: 66  FSLRNLRFSTPTTPKPLLIATPSQPLHVQALVICCKAHGFQIRIRSGGHDYEGLSYVSDV 125

Query: 122 P-----------------------------------IAEKSKTLAFPAGGCPTVGVGGHL 181
           P                                   IAEKS+TLAFPAGGCPTVGVGGH 
Sbjct: 126 PFVLVDLGEFRSIAIDIENNTAWVQSGATLGELHYRIAEKSQTLAFPAGGCPTVGVGGHF 185

Query: 182 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 241
           SGGGHGGL+RKYGLAADHVIDA+LVDANG +LDRK MGEDLFWAIRGGGGGSFGIVL+WK
Sbjct: 186 SGGGHGGLVRKYGLAADHVIDAILVDANGMVLDRKLMGEDLFWAIRGGGGGSFGIVLSWK 245

Query: 242 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLG-GKDFFRPNNLKKGGIRR 301
           I+L+ +PP VTVFTVN++LD++TLKLIH+WQYVASK+D+NL  G       N K+    R
Sbjct: 246 IRLVSIPPIVTVFTVNRELDEDTLKLIHRWQYVASKVDENLFIGITLSTGPNTKERQRTR 305

Query: 302 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 361
           TRTRTVNASFFSLFLG+AN+L+SIMQ+SFPEL L K+DC E +WIES L LAGFTNGESL
Sbjct: 306 TRTRTVNASFFSLFLGKANDLLSIMQQSFPELELAKQDCKETSWIESNLILAGFTNGESL 365

Query: 362 EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 416
           EEALLNRST PSFIGA KVKSDYVKEPIS+TGL+G+W MLRTT+I+SASIVLLPYGGKMN
Sbjct: 366 EEALLNRSTTPSFIGAYKVKSDYVKEPISQTGLQGVWHMLRTTDIDSASIVLLPYGGKMN 425

BLAST of Lsi02G009700 vs. NCBI nr
Match: XP_022927114.1 (cannabidiolic acid synthase-like [Cucurbita moschata])

HSP 1 Score: 583.2 bits (1502), Expect = 1.8e-162
Identity = 310/450 (68.89%), Postives = 338/450 (75.11%), Query Frame = 0

Query: 2   NFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILN 61
           +FLA    SILT   WESSAN  + + FL+CLSLHSP GDDSI NLIY  TNSSFS ILN
Sbjct: 6   SFLALTFASILTLETWESSAN--EHLHFLRCLSLHSPNGDDSISNLIYPQTNSSFSSILN 65

Query: 62  FYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNV 121
           F +RNLRFST TTPKPLLI+TP QPFHVQALVICCK HGFQIRIRSGGHDYEGLSYVS+V
Sbjct: 66  FSLRNLRFSTPTTPKPLLIVTPSQPFHVQALVICCKAHGFQIRIRSGGHDYEGLSYVSDV 125

Query: 122 P-----------------------------------IAEKSKTLAFPAGGCPTVGVGGHL 181
           P                                   IAEKS+TLAFPAGGCPTVGVGGH 
Sbjct: 126 PFVLVDLGEFRSIAIDIENNTAWVQSGATLGELHYRIAEKSQTLAFPAGGCPTVGVGGHF 185

Query: 182 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 241
           SGGGHGGL+RKYGLAADHVIDA+LVDANG +LDRK MGEDLFWAIRGGGGGSFGIVL+WK
Sbjct: 186 SGGGHGGLVRKYGLAADHVIDAILVDANGMVLDRKLMGEDLFWAIRGGGGGSFGIVLSWK 245

Query: 242 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLG-GKDFFRPNNLKKGGIRR 301
           I+                           WQYVASK+D+NL  G       N ++    R
Sbjct: 246 IR---------------------------WQYVASKVDENLFIGITLSTGPNSEERQRTR 305

Query: 302 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 361
           TRTRTVNASFFSLFLG+AN+L+SIMQ+SFPEL L K+DCIE +WIES L LAGFTN ESL
Sbjct: 306 TRTRTVNASFFSLFLGKANDLLSIMQQSFPELELAKQDCIEKSWIESNLILAGFTNRESL 365

Query: 362 EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 416
           EEALLNRST PSFIGA KVKSDYVKEPIS+TGL G+W MLRTT+I+SASIVLLPYGGKMN
Sbjct: 366 EEALLNRSTTPSFIGAYKVKSDYVKEPISQTGLPGVWHMLRTTDIDSASIVLLPYGGKMN 425

BLAST of Lsi02G009700 vs. NCBI nr
Match: KAG6583830.1 (O-acetylstemmadenine oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 502.7 bits (1293), Expect = 3.1e-138
Identity = 262/372 (70.43%), Postives = 292/372 (78.49%), Query Frame = 0

Query: 2   NFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILN 61
           +FLAF   SILT G WESSAN  + + FL+CLSLHSP GDDSI NLIY  TNSSFS ILN
Sbjct: 6   SFLAFTFASILTLGTWESSAN--EHLHFLRCLSLHSPNGDDSISNLIYPQTNSSFSSILN 65

Query: 62  FYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNV 121
           F +RNLRFST TTPKPLLI+TP QPFHVQALVICCK HGFQIRIRSGGHDYEGLSYVS+V
Sbjct: 66  FSLRNLRFSTPTTPKPLLIVTPSQPFHVQALVICCKAHGFQIRIRSGGHDYEGLSYVSDV 125

Query: 122 P-----------------------------------IAEKSKTLAFPAGGCPTVGVGGHL 181
           P                                   IAEKS+TLAFPAGGCPTVGVGGH 
Sbjct: 126 PFVLVDLGEFRSIAIDIENNTAWVQSGATLGELHYRIAEKSQTLAFPAGGCPTVGVGGHF 185

Query: 182 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 241
           SGGGHGGL+RKYGLAADHVIDA+LVDANG +LDRK MGEDLFWAIRGGGGGSFGIVL+WK
Sbjct: 186 SGGGHGGLVRKYGLAADHVIDAILVDANGMVLDRKLMGEDLFWAIRGGGGGSFGIVLSWK 245

Query: 242 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLG-GKDFFRPNNLKKGGIRR 301
           I+L+ +PP VTVFTVN++LD++TLKLIH+WQYVASK+D+NL  G       N ++    R
Sbjct: 246 IRLVSIPPIVTVFTVNRELDEDTLKLIHRWQYVASKVDENLFIGITLSTGPNSEERQRTR 305

Query: 302 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 338
           TRTRTVNAS FSLFLG+AN+L+SIMQ+SFPEL L K+DCIE +WIES L LAGFTNGESL
Sbjct: 306 TRTRTVNASCFSLFLGKANDLLSIMQQSFPELELAKQDCIEKSWIESNLILAGFTNGESL 365

BLAST of Lsi02G009700 vs. NCBI nr
Match: KAA8514840.1 (hypothetical protein F0562_018019 [Nyssa sinensis])

HSP 1 Score: 467.6 bits (1202), Expect = 1.1e-127
Identity = 248/448 (55.36%), Postives = 313/448 (69.87%), Query Frame = 0

Query: 2   NFLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILN 61
           + L+ + V +L    WE+SA+  +  +FLQCLSLHS +   SI N+IYTP N+S+  IL 
Sbjct: 7   SLLSLLFVFLLPIS-WEASAHTHE--DFLQCLSLHS-QHSASISNVIYTPNNASYLPILE 66

Query: 62  FYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNV 121
           F I+NLRFS+ATTPKPL+I+TPL    +QA + C + HG QIR+RSGGHDYEGLSYVS++
Sbjct: 67  FSIQNLRFSSATTPKPLVIVTPLHESQIQATIYCSRNHGMQIRVRSGGHDYEGLSYVSDI 126

Query: 122 P-----------------------------------IAEKSKTLAFPAGGCPTVGVGGHL 181
           P                                   IAEKSKTLAFPAG CPT+GVGGH 
Sbjct: 127 PFIIVDLINLRSITVDAENSTAWVQAGATVGELYYRIAEKSKTLAFPAGVCPTIGVGGHF 186

Query: 182 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 241
           SGGG+G LLRKYGLAAD++IDA L+D NG++LDR+SMGEDLFWAIRGGGG SFG++LAWK
Sbjct: 187 SGGGYGTLLRKYGLAADNIIDARLMDVNGRILDRESMGEDLFWAIRGGGGASFGVILAWK 246

Query: 242 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRT 301
           IKL  VP TVTVFTV + L+QN  K++HQWQYVA K  ++L  +   R  N       + 
Sbjct: 247 IKLAPVPSTVTVFTVRRTLEQNATKIVHQWQYVAHKFPEDLFIRILIRSLNSS-----QD 306

Query: 302 RTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE 361
             RT+ ASF SLFLG  ++LIS+MQ+SFPELGL+KEDCIE +WIESIL  AGF +G S  
Sbjct: 307 EKRTIQASFNSLFLGGVDKLISLMQESFPELGLVKEDCIEMSWIESILYFAGFPSGASF- 366

Query: 362 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 415
           + LL+R+  P      K KSDYVKEPISE+GL+G+WK     ++E+A ++L PYGG+M+E
Sbjct: 367 DVLLDRT--PLARSYFKAKSDYVKEPISESGLEGIWKQFYEEDVEAAEMILSPYGGRMDE 426

BLAST of Lsi02G009700 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 359.0 bits (920), Expect = 5.2e-99
Identity = 202/441 (45.80%), Postives = 267/441 (60.54%), Query Frame = 0

Query: 4   LAFIIVSILTFGI-WESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 63
           L F   S L   I    SAN      FLQCLSL      + +  +I+TP ++SFS +L  
Sbjct: 11  LIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQL-NDSNIVSKVIHTPNDTSFSSVLAS 70

Query: 64  YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 123
            I+N RFS    PKP+LI+TP+QP  VQ+ V C +  G  IR RSGGHDYEGLSYV++ P
Sbjct: 71  SIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKP 130

Query: 124 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 183
                                              I +K++TLAFPAG CPTVGVGGH S
Sbjct: 131 FVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFS 190

Query: 184 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 243
           GGG+G LLRK+GLAADHVIDA +VDA G++L+R+ MGED FWAIRGGGG SF +VL+WKI
Sbjct: 191 GGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKI 250

Query: 244 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 303
            L+ VP TVTVF V K  +Q+ LK+IH+WQ+VA K+ D+L    F R        + +  
Sbjct: 251 GLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDL----FIRV-------MLQRY 310

Query: 304 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEE 363
              V ASF  L+LG    L+ ++ K FPELGL ++DC E +WIES++  A     E  EE
Sbjct: 311 KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA-----ELGEE 370

Query: 364 AL--LNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 407
            +  L + T  S   A K KSD+V+EP+ +T +  LW+ L+    E A ++  P+GGKM+
Sbjct: 371 PINVLTKRTRASL--AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMS 430

BLAST of Lsi02G009700 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 358.6 bits (919), Expect = 6.8e-99
Identity = 201/441 (45.58%), Postives = 271/441 (61.45%), Query Frame = 0

Query: 5   AFIIVSILTFGIWESSANYQKPIEFLQCLSLHS-PKGDDSIFNLIYTPTNSSFSHILNFY 64
           A I+V      IW+SS++      F QCL+ +S PK    I   I+   N S+S +L   
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPK--HPISPAIFFSGNGSYSSVLQAN 63

Query: 65  IRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVS---- 124
           IRNLRF+T +TPKP LII      HVQA + C K H  Q++IRSGGHDY+GLSYV+    
Sbjct: 64  IRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGK 123

Query: 125 -------------NVPIA--------------------EKSKTLAFPAGGCPTVGVGGHL 184
                        +V +A                    EKSKTLA+PAG CPTVGVGGH+
Sbjct: 124 PFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHI 183

Query: 185 SGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWK 244
           SGGG+G ++RKYGL  D+ IDA +VD NGK+LDRK MGEDL+WAI GGGGGS+G+VLA+K
Sbjct: 184 SGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYK 243

Query: 245 IKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRP-NNLKKGGIRR 304
           I L+ VP  VTVF +++ L+QN   +IH+WQ VA KL D L    F R   ++  G +  
Sbjct: 244 INLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDEL----FIRTVIDVVNGTV-- 303

Query: 305 TRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESL 364
           +  +TV  +F ++FLG    L+SI+ + FPELGL++ DC E +WI+S+L       G S 
Sbjct: 304 SSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS- 363

Query: 365 EEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMN 407
           E  LL R+   +++   K KSDYV+EPIS TGL+ +WK  +   +E  ++   PYGG+M 
Sbjct: 364 ETLLLQRNQPVNYL---KRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMG 423

BLAST of Lsi02G009700 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 345.1 bits (884), Expect = 7.8e-95
Identity = 191/435 (43.91%), Postives = 264/435 (60.69%), Query Frame = 0

Query: 8   IVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSS-FSHILNFYIRN 67
           +V++L   +  SS+  Q+  +F++CL  +S        +      N++ F   L    +N
Sbjct: 14  LVTLLLISVPLSSSTLQQ--DFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 73

Query: 68  LRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSN------ 127
           LR+ T + PKP+ I  PL   HVQA V+C K     +R+RSGGHDYEGLS+V+       
Sbjct: 74  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 133

Query: 128 -------------------------------VPIAEKSKTLAFPAGGCPTVGVGGHLSGG 187
                                            I EKS+T  FPAG C ++G+GGHL GG
Sbjct: 134 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 193

Query: 188 GHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKIKL 247
            +G ++RK+GL AD+V+DA +VDANG++LDR +MGED+FWAIRGGGGGSFG++LAWKIKL
Sbjct: 194 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 253

Query: 248 LYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTRTR 307
           + VP TVTVFTV K L+Q+  K++++W+ +A KLDD+L    F R          +   R
Sbjct: 254 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDL----FIRVIISPASKTTKPGNR 313

Query: 308 TVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLEEAL 367
           T++ S+ + FLG +N L+ +MQKSFPELGL K+DC E +WI+S++ +AGF N  + E  L
Sbjct: 314 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL 373

Query: 368 LNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNEISE 405
             +S    F    K KSD+VKEPI   GL+GLW+  R    +S   +  PYGG M+ ISE
Sbjct: 374 AGKSL---FKNHFKAKSDFVKEPIPVEGLEGLWE--RFLEEDSPLTIWNPYGGMMSRISE 433

BLAST of Lsi02G009700 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 344.7 bits (883), Expect = 1.0e-94
Identity = 197/459 (42.92%), Postives = 277/459 (60.35%), Query Frame = 0

Query: 1   MNFLAFIIVSILTFGIWES---SANYQKPIEFLQCLSLHSPKGDDSIF-NLIYTPTNSSF 60
           M F +F+  +IL F +  S   SA+     +FL+CLS      D+++   +I+T  +SSF
Sbjct: 1   MEFSSFLF-TILLFSLNISPLVSAHGSNHEDFLKCLSYR--MNDNTVEPKVIHTSKDSSF 60

Query: 61  SHILNFYIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLS 120
             IL+  I+N RFS + TPKP+ IITP++   VQ ++ C ++HG  +R RS GH YEGLS
Sbjct: 61  FSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLS 120

Query: 121 YVS-NVP-----------------------------------IAEKSKTLAFPAGGCPTV 180
           Y++ N P                                   I + +K+LAFPAG  PTV
Sbjct: 121 YIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTV 180

Query: 181 GVGGHLSGGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFG 240
           GVGG  SGGG+G LLRKYGLAAD++IDAL+VDA+G++LDR++MGED FWAIRGGGG SFG
Sbjct: 181 GVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFG 240

Query: 241 IVLAWKIKLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKK 300
           ++L+WK+KL+ VP T+TVF V K   +  +++I +WQY A K+ D+L    F R      
Sbjct: 241 VILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDL----FIRTT---- 300

Query: 301 GGIRRTRTRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFT 360
             + R+    V+A F  L++G  N L+++M++ FPELGL KE C E +WIES+L  A F 
Sbjct: 301 --LERSNKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFP 360

Query: 361 NGESLEEALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPY 419
            GESL        T+ SF G    K D+V+EPI E  ++ +W+ L         I+L P+
Sbjct: 361 KGESLGVLTNRERTSLSFKG----KDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPF 420

BLAST of Lsi02G009700 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 342.4 bits (877), Expect = 5.1e-94
Identity = 191/454 (42.07%), Postives = 266/454 (58.59%), Query Frame = 0

Query: 3   FLAFIIVSILTFGIWESSANYQKPIEFLQCLSLHSPKGDDSIFNLIYTPTNSSFSHILNF 62
           F  F +VS+  F      ++      F+QC S  +      I + +++ TN SFS +L  
Sbjct: 12  FFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRA 71

Query: 63  YIRNLRFSTATTPKPLLIITPLQPFHVQALVICCKVHGFQIRIRSGGHDYEGLSYVSNVP 122
           YIRN RF+T++TPKP +I+TP    HV A V C K   F ++IRSGGHDYEGLSY+S+ P
Sbjct: 72  YIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKP 131

Query: 123 -----------------------------------IAEKSKTLAFPAGGCPTVGVGGHLS 182
                                              I EKSK   FPAG CPTVGVGGH+S
Sbjct: 132 FFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHIS 191

Query: 183 GGGHGGLLRKYGLAADHVIDALLVDANGKLLDRKSMGEDLFWAIRGGGGGSFGIVLAWKI 242
           GGG+G +LRK+GL+ D++IDA +VD NG++LDRKSMGEDLFWAI GGGG SFG+VL +K+
Sbjct: 192 GGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKV 251

Query: 243 KLLYVPPTVTVFTVNKKLDQNTLKLIHQWQYVASKLDDNLGGKDFFRPNNLKKGGIRRTR 302
           KL+ VP TVTVF V K +D   + ++H+WQ V  K D NL  +   +P       + R +
Sbjct: 252 KLVPVPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQP-------VTRKK 311

Query: 303 TRTVNASFFSLFLGQANELISIMQKSFPELGLLKEDCIENNWIESILTLAGFTNGESLE- 362
            +TV A+  +LFLG+A E+++++ K FPEL L KE+C E  W +S L      N   ++ 
Sbjct: 312 VKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDP 371

Query: 363 EALLNRSTNPSFIGANKVKSDYVKEPISETGLKGLWKMLRTTNIESASIVLLPYGGKMNE 421
           +  L+R+ + +  G  K KSDYV   I   G++ L+K  + T +    +V  PYGGKM E
Sbjct: 372 KVFLDRNLDRANFG--KRKSDYVASEIPRDGIESLFK--KMTELGKIGLVFNPYGGKMAE 431