Lsi02G008020 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G008020 vs. ExPASy Swiss-Prot
Match: O81787 (Plant-specific TFIIB-related protein PTF2 OS=Arabidopsis thaliana OX=3702 GN=PTF2 PE=1 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 5.9e-129
Identity = 249/532 (46.80%), Postives = 350/532 (65.79%), Query Frame = 0

Query: 5   CKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGSVLNY 64
           CK C+  +  RD+ +GN  C  CG ++E+DNYEAQL GI GPQGT++RVGT G GSVL+Y
Sbjct: 3   CKRCNGSNFERDEDTGNSYCGGCGTLREYDNYEAQLGGIRGPQGTYIRVGTIGRGSVLDY 62

Query: 65  KDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAYVSMR 124
           KDKKI+EA  +IE+ T RL    +K+  ++ ++S +T+GE+G G+WFPIL+GAC Y  +R
Sbjct: 63  KDKKIYEANNLIEETTERLNL-GNKTEVIKSMISKLTDGEFGQGEWFPILIGACCYAVVR 122

Query: 125 KDNR-PLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNSPSF 184
           ++ +  L M EVA  V CDLH+LG M+ RVVD LDL   E   FD+VG   +   NSP  
Sbjct: 123 EEGKGVLSMEEVAYEVGCDLHQLGPMIKRVVDHLDL---ELREFDLVGLFTKTVTNSPRL 182

Query: 185 SSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNVAME 244
           + VD D  E+I+KQG FL+ CA+KWFL+TGR+P+P+V AVL  V ++N V V ID++A +
Sbjct: 183 TDVDRDKKEKIIKQGTFLMNCALKWFLSTGRRPMPLVVAVLAFVVQVNGVKVKIDDLAKD 242

Query: 245 VHAKVSTCKKRYRELLEALVEVGKK--LPWGKDITTKNIVKNAPFVIQYMELKSM-SNAS 304
               ++TCK RY+EL E LV+V ++  LPW KD+T KN++K++  +   ME KSM     
Sbjct: 243 ASVSLTTCKTRYKELSEKLVKVAEEVGLPWAKDVTVKNVLKHSGTLFALMEAKSMKKRKQ 302

Query: 305 GKGKDLENV-GIDLQSAVSECLRKELDYESEVNNLEDD-SQYFELQGSSWHDESSCDNGN 364
           G GK+L    G  ++  V +CL KE  Y  + +  +D  S+YF+++G      S C+  +
Sbjct: 303 GTGKELVRTDGFCVEDLVMDCLSKESMYCYDDDARQDTMSRYFDVEGE--RQLSLCNYDD 362

Query: 365 KLNISHECLSLIYHKFLDEMA----HLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSEL 424
             NIS   LS  Y++F D +       R  G  + ++ R+     G+ S+  WW+GKSEL
Sbjct: 363 --NISENQLSTKYNEFEDRVCGGTLAKRSQGSSQSMWQRRS--VFGMVSTENWWKGKSEL 422

Query: 425 SKKLLLQQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPS-SAPTGDVN 484
           SK+LLL+ LLE D+G + +PPS++  C A +RR+EK+ AAK RI  I HPS +   G ++
Sbjct: 423 SKRLLLKDLLEKDVGLEALPPSYIKGCVAVERRREKIKAAKLRINAIQHPSDNVSEGALS 482

Query: 485 TE---KKRKRKGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
            E    K+KRK  + I+WED++I+TL+LH V EEEIEKGHY  LLDLHVFNS
Sbjct: 483 LELEHSKKKRKKGSEIDWEDLVIQTLVLHNVNEEEIEKGHYKTLLDLHVFNS 524

BLAST of Lsi02G008020 vs. ExPASy Swiss-Prot
Match: Q4R318 (Transcription factor IIIB 50 kDa subunit OS=Macaca fascicularis OX=9541 GN=BRF2 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.2e-09
Identity = 65/309 (21.04%), Postives = 133/309 (43.04%), Query Frame = 0

Query: 3   GSCKNCHSRSLFRDD--VSGNLICSSCG-IVQE-------FDNYEAQLCGINGPQGTFVR 62
           G C +C S  L  D       L+CS CG +V E        D    +    +   G   +
Sbjct: 5   GRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTFSDEGNLREVTYSRSTGENEQ 64

Query: 63  VGTSGSGSVLNYKDK-KIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF 122
           +  S    +   +D  ++ +     ED        A + + +R   + + + E       
Sbjct: 65  ISRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYRHSGIR--AARLQKKE------- 124

Query: 123 PILVGACAYVSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVG 182
            +LVG C  ++ R+ N PL M  + + +  DL     + +++V  L L  P   + ++V 
Sbjct: 125 -VLVGCCVLITCRQHNWPLTMGAICTLLYADLDVFSSIYMQIVKLLGLDMPSLCLAELVK 184

Query: 183 ---SLERAARNSPSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVL-- 242
              S  +  + SPS  +   +  E+++ + + L++ A + +L TGR PLP++ A   L  
Sbjct: 185 TYCSSFKLFQASPSVPAKYVEDKEKMLSRTLQLVELANETWLVTGRHPLPVITAATFLAW 244

Query: 243 --VSKLNEVNVSIDNVAMEVHAKVS-TCKKRYRELLEALVEVGKKLPWGK--DITTKNIV 291
             +   + ++ S+       +  +      R +ELL  L+ + ++L W +   +  +++V
Sbjct: 245 QSLQPADRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLRVLRLDKRSVV 303

BLAST of Lsi02G008020 vs. ExPASy Swiss-Prot
Match: Q9HAW0 (Transcription factor IIIB 50 kDa subunit OS=Homo sapiens OX=9606 GN=BRF2 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.6e-09
Identity = 66/311 (21.22%), Postives = 133/311 (42.77%), Query Frame = 0

Query: 3   GSCKNCHSRSLFRDD--VSGNLICSSCG-IVQE-------FDNYEAQLCGINGPQGTFVR 62
           G C +C S  L  D       L+CS CG +V E        D    +    +   G   +
Sbjct: 5   GRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTFSDEGNLREVTYSRSTGENEQ 64

Query: 63  VGTSGSGSVLNYKDK-KIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF 122
           V  S    +   +D  ++ +     ED        A + + +R   + + + E       
Sbjct: 65  VSRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYRHSGIR--AARLQKKE------- 124

Query: 123 PILVGACAYVSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVG 182
            +LVG C  ++ R+ N PL M  + + +  DL       +++V  L L  P   + ++V 
Sbjct: 125 -VLVGCCVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLLGLDVPSLCLAELVK 184

Query: 183 ---SLERAARNSPSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVL-- 242
              S  +  + SPS  +   +  E+++ + + L++ A + +L TGR PLP++ A   L  
Sbjct: 185 TYCSSFKLFQASPSVPAKYVEDKEKMLSRTMQLVELANETWLVTGRHPLPVITAATFLAW 244

Query: 243 --VSKLNEVNVSIDNVAMEVHAKVS-TCKKRYRELLEALVEVGKKLPWGK--DITTKNIV 293
             +   + ++ S+       +  +      R +ELL  L+ + ++L W +   +  +++V
Sbjct: 245 QSLQPADRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLRVLRLDKRSVV 304

BLAST of Lsi02G008020 vs. ExPASy Swiss-Prot
Match: Q3UAW9 (Transcription factor IIIB 50 kDa subunit OS=Mus musculus OX=10090 GN=Brf2 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 6.1e-09
Identity = 66/307 (21.50%), Postives = 128/307 (41.69%), Query Frame = 0

Query: 5   CKNCHSRSLFRDD--VSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRV----GTSGS 64
           C +C S  L  D       L+CS CG V      E  L      +G F  V     T  +
Sbjct: 7   CPDCGSSELVEDSHYSQSQLVCSDCGCVVT----EGVLTTTFSDEGNFREVTYSRSTGEN 66

Query: 65  GSVLNYKDKKIFEAQKIIEDITFRLGF--SASKSNDVRILVSTITEGEYGLGDWFPILVG 124
             V   + + +   + +   +   L F  +A         +S I        +   +LVG
Sbjct: 67  EQVSRCQQRDLRRVRDLCRILKLPLTFEDTAISYYQKAYQLSGIRAARLQKKE---VLVG 126

Query: 125 ACAYVSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSL--- 184
            C  ++ R+ N PL M  + + +  DL       +++V  L L  P   + D+V S    
Sbjct: 127 CCVLITCRQHNWPLTMGTICTLLYADLDLFSGTYMQMVKLLGLDVPSLCLADLVKSYCSS 186

Query: 185 ERAARNSPSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVL----VSK 244
            +  + SPS  +   +  ++++ + + L++ A + +L TGR PLP++ A   L    +  
Sbjct: 187 FKLFQASPSVPAKYVEDKDKMLSRTLLLVELADETWLVTGRHPLPIITAATFLAWQSLRP 246

Query: 245 LNEVNVSIDNVAMEVHAKVS-TCKKRYRELLEALVEVGKKLPWGK--DITTKNIVKNAPF 294
            + +  S+       +  +      R +ELL  L+++  +L W +   +  +++VK+   
Sbjct: 247 SDRLTCSLAQFCKLANVDLPYPAASRLQELLAVLLQMAGQLAWLQVLKLNKRSVVKHIGD 306

BLAST of Lsi02G008020 vs. ExPASy Swiss-Prot
Match: Q29S07 (Transcription factor IIIB 50 kDa subunit OS=Bos taurus OX=9913 GN=BRF2 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.3e-08
Identity = 66/312 (21.15%), Postives = 129/312 (41.35%), Query Frame = 0

Query: 3   GSCKNCHSRSLFRDD--VSGNLICSSCG-IVQE-------FDNYEAQLCGINGPQGTFVR 62
           G C +C S  L  D       L+CS CG +V E        D    +    +   G   +
Sbjct: 5   GRCPDCGSAELVEDSHYSQNQLVCSDCGCVVTEGVLTTTFSDEGNLREVTYSRSTGENEQ 64

Query: 63  VGTSGSGSVLNYKDK-KIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWF 122
           V  S    +   +D  ++ +     ED        A +   +R   + + + E       
Sbjct: 65  VSRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAHQLAGIR--TARLQKKE------- 124

Query: 123 PILVGACAYVSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVG 182
            +L G C  ++ R+ N PL M  + + +  DL       +++V  L L  P   + D+V 
Sbjct: 125 -VLAGCCVLITCRQRNWPLTMGTICTLLYADLDVFSGTYMQIVKLLGLDVPSLCLVDLVK 184

Query: 183 ---SLERAARNSPSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVL-- 242
              S  +    SPS  +   +  E+++ + + L++ A + +L TGR PLP++ A   L  
Sbjct: 185 TYCSSFKLFEASPSVPAKYVEDKEKMLSRTLQLVELADETWLVTGRHPLPVITAATFLAW 244

Query: 243 --VSKLNEVNVSIDNVAMEVHAKVS-TCKKRYRELLEALVEVGKKLPWGK--DITTKNIV 294
             +   + +  S+       +  +      R +ELL  L+ + ++L W +   +  +++V
Sbjct: 245 QSLRPSDRLTCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLQVLKLDKRSVV 304

BLAST of Lsi02G008020 vs. ExPASy TrEMBL
Match: A0A5A7UMA0 (Transcription factor IIIB 50 kDa subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002190 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 480/522 (91.95%), Postives = 505/522 (96.74%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCK+C+SRS+FRDDVSGNLICSSCGIVQEF+NYEAQL GINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKK++EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPL MSEVASAVECDLHELGRMV+RVVDFLDL+G EFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS ++AD LERIVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVSKLNEV+VS++NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           GKGKDLENVGIDLQSAVSECLRKEL+YESEVNNLEDDSQYFELQGS W DES+CDNGN+L
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY+KFLDEMA LR SGGI +VYGRKQGRKAG YSSTEWWEGKS LSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRI+RIMHPS+AP GDVN +KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDLHVF S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTS 522

BLAST of Lsi02G008020 vs. ExPASy TrEMBL
Match: A0A1S3B6X4 (transcription factor IIIB 50 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103486826 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 480/522 (91.95%), Postives = 505/522 (96.74%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCK+C+SRS+FRDDVSGNLICSSCGIVQEF+NYEAQL GINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKK++EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPL MSEVASAVECDLHELGRMV+RVVDFLDL+G EFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS ++AD LERIVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVSKLNEV+VS++NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           GKGKDLENVGIDLQSAVSECLRKEL+YESEVNNLEDDSQYFELQGS W DES+CDNGN+L
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY+KFLDEMA LR SGGI +VYGRKQGRKAG YSSTEWWEGKS LSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRI+RIMHPS+AP GDVN +KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDLHVF S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTS 522

BLAST of Lsi02G008020 vs. ExPASy TrEMBL
Match: A0A0A0M0A0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G477510 PE=4 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.6e-270
Identity = 471/522 (90.23%), Postives = 495/522 (94.83%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCKNCHSRS+FRDDVSGN ICSSCGIVQEFDNY+AQL GINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKI+EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKD+RPL MSEVASAVECDLHELGRMV+RVV+FLDL+G EFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS ++AD LERIVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVSKLNEV+VSI+NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
            MEVHA VSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMS AS
Sbjct: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           GKGKDLENV IDLQSAVSECLRKEL+YESEV NLEDDSQYFELQ S W DES+ DNGN+L
Sbjct: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY+KFLDEMA LR SGGI EVYG KQGRK G YSSTEWWEGKSELSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAY+RRKEKVNAAKKRI+RIMHPS+AP  DVN +KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDL+VF S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 522

BLAST of Lsi02G008020 vs. ExPASy TrEMBL
Match: A0A6J1JZD6 (plant-specific TFIIB-related protein PTF2 OS=Cucurbita maxima OX=3661 GN=LOC111490217 PE=4 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.1e-262
Identity = 457/522 (87.55%), Postives = 483/522 (92.53%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKI+EAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPLPMSEVAS +ECDLHELGRMV+RVVDFLDLKGP FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS V+ADTL+ IVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVS LNEVNVSID+V
Sbjct: 181 PSFSKVEADTLDIIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           G+GK+ E+VGIDLQ+AVSECLRKEL YESEVNNLEDDSQYFEL G+SWHDESSCD+ NKL
Sbjct: 301 GQGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWHDESSCDDRNKL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMAHLR SG I E+YGRK GRKAG  SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGEINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
            QLLETD+G  G+PPSFV+SCNAYKRRKEK+NAAK RI+RIM PS A  GDVN EK+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSHAAIGDVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVF+S
Sbjct: 481 KRAVGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSS 522

BLAST of Lsi02G008020 vs. ExPASy TrEMBL
Match: A0A6J1GSR8 (plant-specific TFIIB-related protein PTF2 OS=Cucurbita moschata OX=3662 GN=LOC111457096 PE=4 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.9e-261
Identity = 454/522 (86.97%), Postives = 482/522 (92.34%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKI+EAQ IIEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQNIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPLPMSEVAS +ECDLHELGRMV+RVVDFLDLKGP FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS V+ADTL+ IVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVS LNEVNVSID+V
Sbjct: 181 PSFSKVEADTLDVIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           G+GK+ E+VGIDLQ+AVSECLRKEL YESEVNNLEDDSQYFEL G+SW DESSCD+ NKL
Sbjct: 301 GRGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMAHLR SGGI E+YGRK GRKAG  SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
            QLLETD+G  G+PPSFV+SCNAYKRRKEK+NAAK RI+RIM PS+   G+VN EK+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSNTAIGNVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVF+S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSS 522

BLAST of Lsi02G008020 vs. NCBI nr
Match: XP_038893651.1 (plant-specific TFIIB-related protein PTF2 [Benincasa hispida])

HSP 1 Score: 991.9 bits (2563), Expect = 2.1e-285
Identity = 495/521 (95.01%), Postives = 505/521 (96.93%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQL GINGP GTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLSGINGPLGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERA RNS
Sbjct: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERATRNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS VDAD LERIVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVSKLNEV+VSIDNV
Sbjct: 181 PSFSRVDADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELLEALV+VGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVKVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           GKGKDLENVG+DLQSAVSECLRKEL+YESEVNNLEDDSQYFELQGSSW DESSCDNGN+L
Sbjct: 301 GKGKDLENVGVDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSSWRDESSCDNGNRL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIYHKFLDEMAHL+ SGGI EVYGRKQGRKAG YSSTEWWEGKSELSKKLLL
Sbjct: 361 NISHECLSLIYHKFLDEMAHLKSSGGINEVYGRKQGRKAGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
           QQLLE DIGSQGIPPSFVSSCNAYKRRKEK+ AAKKRI+RIM PS+AP GDVN EKKRKR
Sbjct: 421 QQLLEIDIGSQGIPPSFVSSCNAYKRRKEKIIAAKKRIQRIMLPSNAPIGDVNIEKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFN 522
           KG NGIEWEDIIIETLLLHGVKEEEIEKGHYN LLDLHVFN
Sbjct: 481 KGTNGIEWEDIIIETLLLHGVKEEEIEKGHYNALLDLHVFN 521

BLAST of Lsi02G008020 vs. NCBI nr
Match: XP_008443157.1 (PREDICTED: transcription factor IIIB 50 kDa subunit [Cucumis melo] >KAA0057033.1 transcription factor IIIB 50 kDa subunit [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 4.7e-277
Identity = 480/522 (91.95%), Postives = 505/522 (96.74%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCK+C+SRS+FRDDVSGNLICSSCGIVQEF+NYEAQL GINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKK++EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPL MSEVASAVECDLHELGRMV+RVVDFLDL+G EFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS ++AD LERIVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVSKLNEV+VS++NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           GKGKDLENVGIDLQSAVSECLRKEL+YESEVNNLEDDSQYFELQGS W DES+CDNGN+L
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY+KFLDEMA LR SGGI +VYGRKQGRKAG YSSTEWWEGKS LSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRI+RIMHPS+AP GDVN +KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDLHVF S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTS 522

BLAST of Lsi02G008020 vs. NCBI nr
Match: XP_004149065.1 (plant-specific TFIIB-related protein PTF2 [Cucumis sativus] >KGN65641.1 hypothetical protein Csa_019679 [Cucumis sativus])

HSP 1 Score: 939.9 bits (2428), Expect = 9.5e-270
Identity = 471/522 (90.23%), Postives = 495/522 (94.83%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCKNCHSRS+FRDDVSGN ICSSCGIVQEFDNY+AQL GINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKI+EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKD+RPL MSEVASAVECDLHELGRMV+RVV+FLDL+G EFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS ++AD LERIVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVSKLNEV+VSI+NV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
            MEVHA VSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMS AS
Sbjct: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           GKGKDLENV IDLQSAVSECLRKEL+YESEV NLEDDSQYFELQ S W DES+ DNGN+L
Sbjct: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY+KFLDEMA LR SGGI EVYG KQGRK G YSSTEWWEGKSELSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAY+RRKEKVNAAKKRI+RIMHPS+AP  DVN +KKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           KGA+ IEWEDIIIETLLLHGV+EEEIEKGHY VLLDL+VF S
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 522

BLAST of Lsi02G008020 vs. NCBI nr
Match: XP_023542889.1 (plant-specific TFIIB-related protein PTF2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915.2 bits (2364), Expect = 2.5e-262
Identity = 457/522 (87.55%), Postives = 483/522 (92.53%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKI+EAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPLPMSEVAS +ECDLHELGRMV+RVVDFLDLKGP FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS V+ADTL+ IVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVS LNEVNVSID+V
Sbjct: 181 PSFSKVEADTLDIIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           G+GK+ E+VGIDLQ+AVSECLRKEL YESEVNNLEDDSQYFEL G+SW DESSCD+ NKL
Sbjct: 301 GRGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMAHLR SGGI E+YGRK GRKAG  SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
            QLLETD+G  G+PPSFV+SCNAYKRRKEK+NAAK RI+RIM PS+A  GDVN EK+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSNAAIGDVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVF S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFGS 522

BLAST of Lsi02G008020 vs. NCBI nr
Match: KAG6573303.1 (Plant-specific TFIIB-related protein PTF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 914.8 bits (2363), Expect = 3.3e-262
Identity = 458/522 (87.74%), Postives = 483/522 (92.53%), Query Frame = 0

Query: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RSLFRDDVSGNLICSSCGIVQEFDNY+AQL G+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKI+EAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNS 180
           VSMRKDNRPLPMSEVAS +ECDLHELGRMV+RVVDFLDLKGP FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNV 240
           PSFS V+ADTL+ IVKQGIFLLQCA+KWFLTTGRQPLPMVAAVLVLVS LNEVNVSID+V
Sbjct: 181 PSFSKVEADTLDVIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300
           AMEVHAKVSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMSN  
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVGIDLQSAVSECLRKELDYESEVNNLEDDSQYFELQGSSWHDESSCDNGNKL 360
           G+GK+ E+VGIDLQ+AVSECLRKEL YESEVNNLEDDSQYFEL G+SW DESSCD+ NKL
Sbjct: 301 GQGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYHKFLDEMAHLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMAHLR SGGI E+YGRK GRKAG  SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPSSAPTGDVNTEKKRKR 480
            QLLETDIG  G+PPSFV+SCNAYKRRKEK+NAAK RI+RIM PS A  GDVN EK+RKR
Sbjct: 421 HQLLETDIGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSHAAIGDVNAEKERKR 480

Query: 481 KGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
           K A GIEWEDIIIETLLLH VKEEEIEKGHYN LLDLHVF+S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSS 522

BLAST of Lsi02G008020 vs. TAIR 10
Match: AT4G35540.1 (zinc ion binding;transcription regulators )

HSP 1 Score: 462.6 bits (1189), Expect = 4.2e-130
Identity = 249/532 (46.80%), Postives = 350/532 (65.79%), Query Frame = 0

Query: 5   CKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLCGINGPQGTFVRVGTSGSGSVLNY 64
           CK C+  +  RD+ +GN  C  CG ++E+DNYEAQL GI GPQGT++RVGT G GSVL+Y
Sbjct: 3   CKRCNGSNFERDEDTGNSYCGGCGTLREYDNYEAQLGGIRGPQGTYIRVGTIGRGSVLDY 62

Query: 65  KDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAYVSMR 124
           KDKKI+EA  +IE+ T RL    +K+  ++ ++S +T+GE+G G+WFPIL+GAC Y  +R
Sbjct: 63  KDKKIYEANNLIEETTERLNL-GNKTEVIKSMISKLTDGEFGQGEWFPILIGACCYAVVR 122

Query: 125 KDNR-PLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERAARNSPSF 184
           ++ +  L M EVA  V CDLH+LG M+ RVVD LDL   E   FD+VG   +   NSP  
Sbjct: 123 EEGKGVLSMEEVAYEVGCDLHQLGPMIKRVVDHLDL---ELREFDLVGLFTKTVTNSPRL 182

Query: 185 SSVDADTLERIVKQGIFLLQCAVKWFLTTGRQPLPMVAAVLVLVSKLNEVNVSIDNVAME 244
           + VD D  E+I+KQG FL+ CA+KWFL+TGR+P+P+V AVL  V ++N V V ID++A +
Sbjct: 183 TDVDRDKKEKIIKQGTFLMNCALKWFLSTGRRPMPLVVAVLAFVVQVNGVKVKIDDLAKD 242

Query: 245 VHAKVSTCKKRYRELLEALVEVGKK--LPWGKDITTKNIVKNAPFVIQYMELKSM-SNAS 304
               ++TCK RY+EL E LV+V ++  LPW KD+T KN++K++  +   ME KSM     
Sbjct: 243 ASVSLTTCKTRYKELSEKLVKVAEEVGLPWAKDVTVKNVLKHSGTLFALMEAKSMKKRKQ 302

Query: 305 GKGKDLENV-GIDLQSAVSECLRKELDYESEVNNLEDD-SQYFELQGSSWHDESSCDNGN 364
           G GK+L    G  ++  V +CL KE  Y  + +  +D  S+YF+++G      S C+  +
Sbjct: 303 GTGKELVRTDGFCVEDLVMDCLSKESMYCYDDDARQDTMSRYFDVEGE--RQLSLCNYDD 362

Query: 365 KLNISHECLSLIYHKFLDEMA----HLRLSGGIKEVYGRKQGRKAGVYSSTEWWEGKSEL 424
             NIS   LS  Y++F D +       R  G  + ++ R+     G+ S+  WW+GKSEL
Sbjct: 363 --NISENQLSTKYNEFEDRVCGGTLAKRSQGSSQSMWQRRS--VFGMVSTENWWKGKSEL 422

Query: 425 SKKLLLQQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIERIMHPS-SAPTGDVN 484
           SK+LLL+ LLE D+G + +PPS++  C A +RR+EK+ AAK RI  I HPS +   G ++
Sbjct: 423 SKRLLLKDLLEKDVGLEALPPSYIKGCVAVERRREKIKAAKLRINAIQHPSDNVSEGALS 482

Query: 485 TE---KKRKRKGANGIEWEDIIIETLLLHGVKEEEIEKGHYNVLLDLHVFNS 523
            E    K+KRK  + I+WED++I+TL+LH V EEEIEKGHY  LLDLHVFNS
Sbjct: 483 LELEHSKKKRKKGSEIDWEDLVIQTLVLHNVNEEEIEKGHYKTLLDLHVFNS 524