Lsi02G007320 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G007320 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.8e-131
Identity = 258/563 (45.83%), Postives = 347/563 (61.63%), Query Frame = 0

Query: 23  KRHCVSYISCRASNGENYQNPQAGGDSVSKFDRRDVLFGLGGLYGVTTLNTNKFALAESL 82
           KR     +SC+A+N  N QN QA     SK DRR+VL GLGGLYGV  + T+ FA A+ +
Sbjct: 39  KRSFARKVSCKATN--NDQNDQA----QSKLDRRNVLLGLGGLYGVAGMGTDPFAFAKPI 98

Query: 83  LSPDVHNCGPAKTPAGEM--DCCPPKINRIIDFTPPIIPKLHVRPALQFVTPKQLEDFQR 142
             PDV  CGPA  P G +  +CCPP   +IIDF  P   KL +RP    V     + + +
Sbjct: 99  APPDVSKCGPADLPQGAVPTNCCPPPSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYK 158

Query: 143 GIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQLGSDPQTDFLVHSNALFFPFHRAYLYF 202
            +E++K L  DDPR+F QQA VHCAYCD AY Q+G  P+ +  +H++ LFFPFHR YLYF
Sbjct: 159 AMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGF-PELELQIHNSWLFFPFHRYYLYF 218

Query: 203 FERILRHFIGDPNFALPFWNWDAPIGMHMPIYYNHNPNSSLYDENRDPRHKAPKILDLNY 262
           FE+IL   I DP FALPFWNWD+P GM +P  Y  +P S LYD+ R   H+ P ++DL+Y
Sbjct: 219 FEKILGKLINDPTFALPFWNWDSPAGMPLPAIY-ADPKSPLYDKLRSANHQPPTLVDLDY 278

Query: 263 DGTTEN-PPYNVVDCNLRWMNKQM--SVKTNRKFLGKVIKLGETPVYDDAGIIEKSPHNN 322
           +GT +N      ++ NL+ M +QM  + K  + F G   + G+ P     G IE +PH  
Sbjct: 279 NGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEP-DPGGGSIEGTPHAP 338

Query: 323 VHDWVGDPNNPHREDMGVFYSAARDPIFYAHHANVDRMWNIWQKMNNDRRHYSDPDWLNF 382
           VH W GD   P+ EDMG FYSA RDPIF+AHH+NVDRMW+IW+ +   R   +D DWL+ 
Sbjct: 339 VHLWTGDNTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDS 398

Query: 383 SFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDIPWLHTKPIPR--------IKRSKDVA 442
            F+FY+E   +VR+KV DCL+++NLGYVY+DVDIPWL +KP PR        + +   VA
Sbjct: 399 GFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVALSKVAKKLGVA 458

Query: 443 EPPTIN---------FPQILNSAITTMVER----QSSGELKENEEEVLVIEDVEFDPSLR 502
                +         FP  L S I+T+V+R    + S + KE+EEE+LVIE +EFD  + 
Sbjct: 459 HAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVA 518

Query: 503 LHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEK--TSLRFVLTEVIEMLGLKDA 558
           + F+V VN VDD     P   EF GSF ++PH  +H++K  T LR  LT+++E +  +D 
Sbjct: 519 VKFDVYVNDVDDLPS-GPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDD 578

BLAST of Lsi02G007320 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.1e-120
Identity = 250/584 (42.81%), Postives = 342/584 (58.56%), Query Frame = 0

Query: 2   TSLSSLLSLSSYFPCK---NIPQNKRHCV---SYISCRA--SNGENYQNPQAGGDS--VS 61
           +SL S  S S++ P K   ++    +H V   S ISC+A  +N  N QN Q    S  + 
Sbjct: 14  SSLISTNSFSTFLPNKSQLSLSGKSKHYVARRSSISCKATNNNNSNNQNEQQEESSRLLG 73

Query: 62  KFDRRDVLFGLGGLYGVTTLNTNKFALAESLLSPDVHNCGPAK-TPAGE--MDCCPPKIN 121
           K DRR++L GLGGLYG TTL+   FA A+ +  PD+  C PA+ TP G   + CCPP   
Sbjct: 74  KLDRRNILIGLGGLYGATTLDRKPFAFADPIAPPDLTTCKPAEITPGGSETVPCCPPVTT 133

Query: 122 RIIDFTPPIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCD 181
           +I  F P +   L   PA   VT + L  F++    ++ L  DDPR+  QQAKVHCAYC+
Sbjct: 134 KIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSMVQQAKVHCAYCN 193

Query: 182 HAYKQLGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMH 241
            AY Q+G     D  VH + LFFPFHR YLYF+ERIL   I DP FALP+WNWD+P+G  
Sbjct: 194 GAYPQVGF-TDNDIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALPYWNWDSPVGFP 253

Query: 242 MPIYYNHNPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNV-VDCNLRWMNKQM--SV 301
           +P  Y  + +S LYD+ R+  H+ P ++DL+Y G  ++      +D NL  M +QM    
Sbjct: 254 IPDIYT-DTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLAIMYRQMVSGA 313

Query: 302 KTNRKFLGKVIKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIF 361
           KT   F G   + G        G IE  PHNN+H WVGDP+  H+EDMG FYSA RDP+F
Sbjct: 314 KTPDLFFGHAYRAGNLNTGKYPGTIENMPHNNIHIWVGDPSQTHQEDMGNFYSAGRDPLF 373

Query: 362 YAHHANVDRMWNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSE-NLGY 421
           YAHHANVDRMWNIW+ +   R+  +D DWL+  F+FYDE   +VR+KV D L+ E  L Y
Sbjct: 374 YAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDENAELVRVKVRDSLEPEKQLRY 433

Query: 422 VYEDVDIPWLHTKPIPRIKRSKDVAEPP----TINFPQILNSAITTMV----ERQSSGEL 481
            YE V +PWL TKP  R  ++K  A+      T  FP  L    T  V     R+ S + 
Sbjct: 434 NYEPVSLPWLFTKPTARKTKNKTKAKVAATQLTSKFPATLVEVTTVEVARPKPRKRSKKE 493

Query: 482 KENEEEVLVIEDVEFDPSLRLHFNVVVNVVDDGDDVC-PGNVEFVGSFKNIPHG--LQHQ 541
           K +EEE+L+I+D+EF+ +  + F+V +N  DD + +      EF GSF ++P G   + +
Sbjct: 494 KVDEEELLIIKDIEFEGTEAVKFDVFIN--DDAESLSRRDKSEFAGSFVHVPQGKTTKAK 553

Query: 542 EKTSLRFVLTEVIEMLGLKDAQKLAVKIDPKTGGDQISIGGIKI 558
            KT+L+  +T+++E LG +D   + V + P+     I+IGG KI
Sbjct: 554 TKTNLKLGITDLLEDLGAEDDSSVLVTLVPRVSNSPITIGGFKI 593

BLAST of Lsi02G007320 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 6.5e-118
Identity = 251/596 (42.11%), Postives = 346/596 (58.05%), Query Frame = 0

Query: 3   SLSSLLSLSSYFPC---KNIPQNKRHCVSYISCRASNGENYQNPQAGGD-SVSKFDRRDV 62
           S +S +S SS +P    ++     R     ++C ++N +N  NP+   + S     RR+V
Sbjct: 16  SSNSKISHSSVYPFLQKQHQSSKLRKPKRQVTCSSNNNQN--NPKEEQELSNIVGHRRNV 75

Query: 63  LFGLGGLYGVTTLNTNKFALAESLLSPDVHNC-GPAKTPAG----EMDCCPPKINRIIDF 122
           L GLGG+YG  TL TN  ALA  +  PD+  C  P+  P+G     ++CCPP   +I DF
Sbjct: 76  LIGLGGIYG--TLATNPSALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDF 135

Query: 123 TPPIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQ 182
             P    L VR A   V  + LE +++  E++K L  +DPRNF QQA +HCAYCD AY Q
Sbjct: 136 KFPSNQPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQ 195

Query: 183 LGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYY 242
           +G  P     VH + LFFPFHR YLYF+ERIL   I DP FALPFWN+DAP GM +P  Y
Sbjct: 196 IGF-PDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIY 255

Query: 243 NHNPNSSLYDENRDPRHKAPKILDLNY-DGTTENPPYNVVDCNLRWMNKQM--SVKTNRK 302
             +  S LYDE R+  H+ P ++DLN+ D  ++     ++  NL  M +Q+  + KT+R 
Sbjct: 256 -ADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRL 315

Query: 303 FLGKVIKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIFYAHHA 362
           FLG   + G+      AG IE  PH  VH W GD    + EDMG+FYSAARDPIFY+HH+
Sbjct: 316 FLGNPYRAGDAEP-QGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHS 375

Query: 363 NVDRMWNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVD 422
           NVDR+W IW+ +   +  ++D DWL   F+FYDE KN+VR+ V D LD + LGY Y+DV 
Sbjct: 376 NVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVP 435

Query: 423 IPWLHTKPIPRIKRSKDVAE----------PPTI------------NFPQILNSAITTMV 482
           IPW   KP+PR  + + + E           PTI             FP +LN+ ++ +V
Sbjct: 436 IPWEKAKPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIV 495

Query: 483 ERQS---SGELKENEEEVLVIEDVEFDPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKN 542
           +R     S + KE EEEVLVIE +EF  ++ + F+V +N  D+ D V  GN EF GSF N
Sbjct: 496 KRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAGSFVN 555

Query: 543 IPHGLQ-HQEK---TSLRFVLTEVIEMLGLKDAQKLAVKIDPKTGGDQISIGGIKI 558
           IPH    H+ K   TSLR  +T+++E L ++    + V + PK G  Q+ I  ++I
Sbjct: 556 IPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 602

BLAST of Lsi02G007320 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.6e-116
Identity = 253/585 (43.25%), Postives = 342/585 (58.46%), Query Frame = 0

Query: 5   SSLLSLSSYFPCKNIPQNKRHCVSYISCRASNGENYQNPQAGGD----SVSKFDRRDVLF 64
           S L   +S+ P     +++R   S +SC ++NG+   N  +  D    S  K DRR+VL 
Sbjct: 26  SPLFQRASHVPVAR-NRSRRFAPSKVSCNSANGD--PNSDSTSDVRETSSGKLDRRNVLL 85

Query: 65  GLGGLYGVT--TLNTNKFALAESLLSPDVHNCGPAKTPAG--EMDCCPPKINRIIDF-TP 124
           G+GGLYG       T   A    + +PD+  CG A  P G    +CCPP   +IIDF  P
Sbjct: 86  GIGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLP 145

Query: 125 PIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQLG 184
                +  RPA   V+ + L  +++ IE+ K L  DDPR+F QQA VHC YC  AY Q+G
Sbjct: 146 SSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVG 205

Query: 185 SDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYYNH 244
                +  VH++ LF PFHR YLYF ERIL   I DP FALP+W WD P GM+MP  Y  
Sbjct: 206 Y-TDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYAS 265

Query: 245 NPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDC-NLRWMNKQM--SVKTNRKFL 304
           +P SSLYDE R+ +H  P ++DL+YDGT    P + +   NL  M KQ+     T + FL
Sbjct: 266 SP-SSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFL 325

Query: 305 GKVIKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIFYAHHANV 364
           G   + G+  +   AG +E +PHN VH W G  + P  EDMG FY+A RDPIF+ HHANV
Sbjct: 326 GYPYRAGDA-IDPGAGTLEHAPHNIVHKWTGLADKP-SEDMGNFYTAGRDPIFFGHHANV 385

Query: 365 DRMWNIWQKM-NNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDI 424
           DRMWNIW+ +   +R+ ++D DWL+ +FVFYDE K +V++KVSDC+D+  L Y Y+D+ I
Sbjct: 386 DRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPI 445

Query: 425 PWL----HTKPIPRIKRSKD----VAEPP--TI----NFPQILNSAITTMVER---QSSG 484
           PWL      K     K SK      AE P  TI    +FP+ LNS I   V R     S 
Sbjct: 446 PWLPKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSK 505

Query: 485 ELKENEEEVLVIEDVEFDPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHG--LQH 544
           + KE+EEEVL+I+ +E D    + F+V +N  +D     P N EF GSF N+PH    + 
Sbjct: 506 KEKEDEEEVLLIKGIELDRENFVKFDVYIN-DEDYSVSRPKNSEFAGSFVNVPHKHMKEM 565

Query: 545 QEKTSLRFVLTEVIEMLGLKDAQKLAVKIDPKTGGDQISIGGIKI 558
           + KT+LRF + E++E LG +D + + V I P+ GGD ++IGGI+I
Sbjct: 566 KTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEI 602

BLAST of Lsi02G007320 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.5e-114
Identity = 245/582 (42.10%), Postives = 336/582 (57.73%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCKNIPQNKRHCVSYISCRASNGENYQNPQAGGDSVSKFDRRDVLF 60
           ++S S LL+    FP    P   R  +  +SC+ASN +N   P    +     DRR++L 
Sbjct: 4   LSSQSKLLATPYSFPYHTKP--SRVSLRRVSCKASN-DNKDKPN-DQEKTFSIDRRNMLI 63

Query: 61  GLGGLYGVTTL-NTNKFALAESLLSPDV-HNCGPAKTPAGEMD--CCPPKINRIIDFTPP 120
           GLGGLYG + +  +N+  LA  +  P +  +C P +  A  ++  CCPP +   IDF  P
Sbjct: 64  GLGGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVNVLCCPPDVKDPIDFQMP 123

Query: 121 IIP-KLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQLG 180
             P +L +RPA     P  +E +++ +  +K L  +DPR+FYQQA +HCAYC+ AY Q+G
Sbjct: 124 SNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQVG 183

Query: 181 SDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYYNH 240
             P  +  VH + LF PFHR YLYF+ERIL   I DP FA+PFWNWDAP GMHMP  +  
Sbjct: 184 F-PDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMPHMF-I 243

Query: 241 NPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYN--VVDCNLRWMNKQ-MSVKTNRKFL 300
           +PNS LYD  R+P H    I+DL++  + E P +N   +  NL  M KQ +  K  R F 
Sbjct: 244 DPNSPLYDAKRNPAHFPDTIVDLDF-SSGEAPSHNPRQIGNNLSIMYKQVVRAKKARLFH 303

Query: 301 GKVIKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIFYAHHANV 360
           G+ ++ G  P     G +E +PH N+H W GDP   + E+MG FYSA RDP+FYAHHANV
Sbjct: 304 GRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANV 363

Query: 361 DRMWNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDIP 420
           DRMW IW K +  R+ Y   DWLN  F+ +DE    VR+ V D LD   LGY Y++VDIP
Sbjct: 364 DRMWYIW-KNSLGRKDYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEVDIP 423

Query: 421 WLHTKPIPRIK-------RSKDVAEPPTIN-FPQILNSAITTMVER---QSSGELKENEE 480
           W+++KP PR         RSK       IN FP  L+S ++  V+R     S   KE+EE
Sbjct: 424 WINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKSRSKSEKEDEE 483

Query: 481 EVLVIEDVEFDPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEK------ 540
           EVLVIE ++ +P   L F+V +N  +D D       EF GSF N+PH  +H +K      
Sbjct: 484 EVLVIEKIKHEPQFPLKFDVYIN-DEDEDPSAADQTEFAGSFVNVPHFHRHGDKKDKRQT 543

Query: 541 TSLRFVLTEVIEMLGLKDAQKLAVKIDPKTGGDQISIGGIKI 558
           T+L   ++EV++ L +     + V + P+ G  QI+IGG KI
Sbjct: 544 TNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKI 576

BLAST of Lsi02G007320 vs. ExPASy TrEMBL
Match: A0A5A7UPD3 (Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00590 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.9e-231
Identity = 401/566 (70.85%), Postives = 452/566 (79.86%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK---NIPQN-KRHCVSYISCRASNGENYQNPQAGGDSVSKFDRR 60
           M SLSSLLSLS Y  CK   +IP   K   ++    +    +N+QNPQA GDS  KFDRR
Sbjct: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60

Query: 61  DVLFGLGGLYGVTTLNTNKFALA---ESLLSPDVHNCGPAKTPAGEMDCCPPKINRIIDF 120
           DVLFGLGGLYGVTTL +NKFALA   +  LSPDV NCGPA TP G MDCCPPKIN+IIDF
Sbjct: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120

Query: 121 TPPIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQ 180
           T PI PKLHVRPA+Q +TPKQL DF+RGIEI+KNLK DDPRNFYQQAK+HCAYCD AYKQ
Sbjct: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180

Query: 181 LGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYY 240
           +GS+ QTDFL                    ILRHFIGDP+FA+PFWNWDAPIGMHMPI+Y
Sbjct: 181 IGSE-QTDFL--------------------ILRHFIGDPDFAIPFWNWDAPIGMHMPIHY 240

Query: 241 NHNPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFLG 300
           N NPNS LYD NR+P HKAPK+LDLNYDG+TENPPY+VVDCNLRWMNKQMSVKT  +FLG
Sbjct: 241 NGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLG 300

Query: 301 KVIKLGETPVYDDAGIIEKSPHNNVHDWVGD-PNNPHREDMGVFYSAARDPIFYAHHANV 360
           KV KLGETP  D  G IE SPHNNVH+WVG+ P  P+ EDMG FY+AARDPIFYAHHANV
Sbjct: 301 KVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANV 360

Query: 361 DRMWNIWQKMNNDRRHY-SDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDI 420
           DR+W IW KMN+ R  Y    DWLN +FVFYDE +N VRIKVSDCLDSENLGYVY+DV+I
Sbjct: 361 DRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEI 420

Query: 421 PWLHTKPIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEEVLVIEDVEF 480
           PWL+TK +PR KRS    EPP INFPQ L+SAITT VER S  E +ENEEEVLVIED+E+
Sbjct: 421 PWLNTKQVPRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEY 480

Query: 481 DPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEMLGL 540
           DP+  LHFNV+V+VVD+GDDVCPGNVEF+GSFKNIPHG +H+ K+SLRFVLTE IE LGL
Sbjct: 481 DPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGL 540

Query: 541 KDAQKLAVKIDPKTGGDQISIGGIKI 558
            DA+KLAV+IDPK  GDQI +GGIKI
Sbjct: 541 HDARKLAVRIDPKLNGDQIKVGGIKI 545

BLAST of Lsi02G007320 vs. ExPASy TrEMBL
Match: A0A1S3BFK3 (polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489307 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.9e-231
Identity = 401/566 (70.85%), Postives = 452/566 (79.86%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK---NIPQN-KRHCVSYISCRASNGENYQNPQAGGDSVSKFDRR 60
           M SLSSLLSLS Y  CK   +IP   K   ++    +    +N+QNPQA GDS  KFDRR
Sbjct: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60

Query: 61  DVLFGLGGLYGVTTLNTNKFALA---ESLLSPDVHNCGPAKTPAGEMDCCPPKINRIIDF 120
           DVLFGLGGLYGVTTL +NKFALA   +  LSPDV NCGPA TP G MDCCPPKIN+IIDF
Sbjct: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120

Query: 121 TPPIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQ 180
           T PI PKLHVRPA+Q +TPKQL DF+RGIEI+KNLK DDPRNFYQQAK+HCAYCD AYKQ
Sbjct: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180

Query: 181 LGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYY 240
           +GS+ QTDFL                    ILRHFIGDP+FA+PFWNWDAPIGMHMPI+Y
Sbjct: 181 IGSE-QTDFL--------------------ILRHFIGDPDFAIPFWNWDAPIGMHMPIHY 240

Query: 241 NHNPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFLG 300
           N NPNS LYD NR+P HKAPK+LDLNYDG+TENPPY+VVDCNLRWMNKQMSVKT  +FLG
Sbjct: 241 NGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLG 300

Query: 301 KVIKLGETPVYDDAGIIEKSPHNNVHDWVGD-PNNPHREDMGVFYSAARDPIFYAHHANV 360
           KV KLGETP  D  G IE SPHNNVH+WVG+ P  P+ EDMG FY+AARDPIFYAHHANV
Sbjct: 301 KVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANV 360

Query: 361 DRMWNIWQKMNNDRRHY-SDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDI 420
           DR+W IW KMN+ R  Y    DWLN +FVFYDE +N VRIKVSDCLDSENLGYVY+DV+I
Sbjct: 361 DRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEI 420

Query: 421 PWLHTKPIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEEVLVIEDVEF 480
           PWL+TK +PR KRS    EPP INFPQ L+SAITT VER S  E +ENEEEVLVIED+E+
Sbjct: 421 PWLNTKQVPRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEY 480

Query: 481 DPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEMLGL 540
           DP+  LHFNV+V+VVD+GDDVCPGNVEF+GSFKNIPHG +H+ K+SLRFVLTE IE LGL
Sbjct: 481 DPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGL 540

Query: 541 KDAQKLAVKIDPKTGGDQISIGGIKI 558
            DA+KLAV+IDPK  GDQI +GGIKI
Sbjct: 541 HDARKLAVRIDPKLNGDQIKVGGIKI 545

BLAST of Lsi02G007320 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 5.4e-192
Identity = 336/564 (59.57%), Postives = 419/564 (74.29%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK---NIPQNKRH-CVSYISCRASNGENYQNPQAGGDSVSKFDRR 60
           M SLSSLL LS Y   K   +I  NKRH  V+Y+SC+A++  N      G DSVSKFDRR
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAASSNN------GEDSVSKFDRR 60

Query: 61  DVLFGLGGLYGVTTLNTNKFALAESLLSPDVHNCGPAKTPAGEMDCCPPKINRIIDFTPP 120
           +VLFGLGGLYGV T   +  A   + L+PDV NC  A+TP G  +CCPP+I +II F PP
Sbjct: 61  EVLFGLGGLYGVVTATGSVLA---APLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFVPP 120

Query: 121 IIPKLHV-RPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQLG 180
            I + +V RPA   +   Q+E+ +RGI+ +K L  DDP +F  QAK+HCAYCD+AY QLG
Sbjct: 121 KIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLG 180

Query: 181 SDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYYNH 240
           S  +T F VHSNALFFPFHRAYLYFFERILRH+IG+P+FA+PFWNWD+P GMHMP +YN 
Sbjct: 181 SITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYN- 240

Query: 241 NPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFLGKV 300
            P+SSLYD  RDPRH   K++DLNY+G TE+  ++VVDCNLRWMN +M V++ R FLG+V
Sbjct: 241 RPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRV 300

Query: 301 IKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIFYAHHANVDRM 360
           +K GETP   D G IE+SPHNN+HDWVG    PH EDMG FYSAA DP+FYAHH N+DR+
Sbjct: 301 LKAGETPKPSDMGTIEQSPHNNIHDWVG-TKTPHFEDMGSFYSAALDPLFYAHHTNIDRL 360

Query: 361 WNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDIPW-- 420
           WNIW+ ++ + + ++DPDWLN SFVFYDE KN VRI+VSDCLD+E LGYVY++V +PW  
Sbjct: 361 WNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLL 420

Query: 421 LHTKPIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEEVLVIEDVEFDP 480
           L+  P P      +  EPP INFPQ LNS ITTMV+R       E  + VLV+EDVE+DP
Sbjct: 421 LNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDVVLVLEDVEYDP 480

Query: 481 SLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEMLGLKD 540
           + R+HF+V+VNVVD+  D C   +EFVGSFKNIPHGL H +KTSLRFV+TEVI+ LGL+ 
Sbjct: 481 TTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLER 540

Query: 541 AQKLAVKIDPKTGGDQISIGGIKI 558
            + LAVKI+P  GG+ +++GGIKI
Sbjct: 541 DKNLAVKIEPMVGGEGVTLGGIKI 553

BLAST of Lsi02G007320 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 9.2e-192
Identity = 335/564 (59.40%), Postives = 417/564 (73.94%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK---NIPQNKRH-CVSYISCRASNGENYQNPQAGGDSVSKFDRR 60
           M SLSSLL LSSY   K   +I  NKRH  V+Y+SC+A++         G DSVSKFDRR
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNN-------GEDSVSKFDRR 60

Query: 61  DVLFGLGGLYGVTTLNTNKFALAESLLSPDVHNCGPAKTPAGEMDCCPPKINRIIDFTPP 120
           +VLFGLGGLYGV T  ++  A   + L+PD+ NC  AKTP G  DCCPP+I +II F PP
Sbjct: 61  EVLFGLGGLYGVVTATSSVLA---APLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPP 120

Query: 121 IIPKLHV-RPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQLG 180
            I + +V RPA   +   Q+E+ + GI+ +K L   DP +F  QAK+HCAYCD+ Y QLG
Sbjct: 121 KIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLG 180

Query: 181 SDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYYNH 240
           S  +T F VHSNALFFPFHRAYLYFFERILRH+IG+PNFA+PFWNWD+P GM+MP +YN 
Sbjct: 181 SITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYN- 240

Query: 241 NPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFLGKV 300
            P+SSLYD  RDPRH   K++ LNY+G TE+  ++VVDCNLRWMN +M V++ R FLG+V
Sbjct: 241 RPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRV 300

Query: 301 IKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIFYAHHANVDRM 360
           +K GETP   D G IE+SPHNN+HDW+G    PH EDMG FYSAA DP+FYAHHAN+DR+
Sbjct: 301 LKAGETPNPTDMGTIEQSPHNNIHDWLG-TKTPHFEDMGSFYSAALDPLFYAHHANIDRL 360

Query: 361 WNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDIPWLH 420
           WNIW+ ++ + + + DPDWLN SFVFYDE KN V IKVSDCLD+E LGYVY++V +PWLH
Sbjct: 361 WNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLH 420

Query: 421 TK--PIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEEVLVIEDVEFDP 480
               P P      + +EPP +NFPQILNS ITTMV+R       E  +EVLV+EDVE+DP
Sbjct: 421 MNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDP 480

Query: 481 SLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEMLGLKD 540
           + R+HF+V+VNVVD   D C   +EFVGSFKNIPHGL H +KTSLRFV+TEVI+ LGL+ 
Sbjct: 481 TTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLER 540

Query: 541 AQKLAVKIDPKTGGDQISIGGIKI 558
            + LAVKI+P  GG+ +++GGIKI
Sbjct: 541 DKNLAVKIEPMVGGEGVTVGGIKI 552

BLAST of Lsi02G007320 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 2.2e-185
Identity = 345/570 (60.53%), Postives = 418/570 (73.33%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK----NIPQNKRHCV-SYISCRASNGENYQNPQAGGDSVSKFDR 60
           M SLSSLLS+S Y  CK     IP NKR  V +++SC+ASN         G DSV+KFDR
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNN--------GEDSVNKFDR 60

Query: 61  RDVLFGLGGLYGVTTLNTNKFALAES-LLSPDVHNCGPAKTPA--GEMDCCPPKINRIID 120
           RD+LFGLGGLYGV TL+    ALA+   LSPDVHNC P K  A   +MDCCPPKI  II+
Sbjct: 61  RDMLFGLGGLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIE 120

Query: 121 FTPPIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYK 180
           FTPP++P   VRPA+  +TP+Q+ D ++GI+++K L  DDPR+F  QAKVHCAYCD AYK
Sbjct: 121 FTPPVVPTPRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYK 180

Query: 181 QLGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIY 240
           Q+  D   DF VH NALFFPFHRAYLYFFERIL H +  P+FA+PFWNWDAP GM MP  
Sbjct: 181 QM--DSTMDFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKI 240

Query: 241 YNHNPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFL 300
           Y   P+SSLYDENRD  H+ PK+L+LNY G     P  +VD NLRWMN QMSV+T ++FL
Sbjct: 241 YT-KPSSSLYDENRDKCHQPPKLLNLNYGGKEVKDP-EIVDFNLRWMNDQMSVETAKEFL 300

Query: 301 GKVIKLGETPVYDDAGIIEKSPHNNVHDWVGDPN-NPHREDMGVFYSAARDPIFYAHHAN 360
           GKVIK G  P  + +G IE SPHNN+H WVGDP    H EDMG FYSA RDP+FYAHHAN
Sbjct: 301 GKVIKRGNGP-ENGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHAN 360

Query: 361 VDRMWNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDI 420
           VDRMWNIWQKM +  R ++D DWLN SFV YDE +  VRIKVS CLDS+ LGYVY+DV I
Sbjct: 361 VDRMWNIWQKMKDRPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPI 420

Query: 421 PWLHTKPIPRIKRSKDVAE-PPTINFPQILNSAITTMVERQ--SSGELKENEEEVLVIED 480
           PW  T PI R K+ + +   PPTI+FPQIL+S ITTMV+R   +S E + N+ EVLV+ED
Sbjct: 421 PWKDTPPIRRPKQERSLGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLED 480

Query: 481 VEFDPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEM 540
           VE+DP+L LHF+V++NV D+G+DV  G++EFVG+F N+PHG     KTSL FV+T+ I+ 
Sbjct: 481 VEYDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQ 540

Query: 541 LGLKDAQKLAVKIDPK-TGGDQISIGGIKI 558
           LGL+  +K+AVKI P   GGD+I IGGIKI
Sbjct: 541 LGLEGDKKVAVKIVPNVNGGDRIKIGGIKI 557

BLAST of Lsi02G007320 vs. NCBI nr
Match: XP_008446638.1 (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056970.1 polyphenol oxidase [Cucumis melo var. makuwa] >TYK26396.1 polyphenol oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 810.1 bits (2091), Expect = 1.2e-230
Identity = 401/566 (70.85%), Postives = 452/566 (79.86%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK---NIPQN-KRHCVSYISCRASNGENYQNPQAGGDSVSKFDRR 60
           M SLSSLLSLS Y  CK   +IP   K   ++    +    +N+QNPQA GDS  KFDRR
Sbjct: 1   MASLSSLLSLSPYSQCKPQSSIPSKYKALSINMSHAKPQKEKNHQNPQAQGDSQIKFDRR 60

Query: 61  DVLFGLGGLYGVTTLNTNKFALA---ESLLSPDVHNCGPAKTPAGEMDCCPPKINRIIDF 120
           DVLFGLGGLYGVTTL +NKFALA   +  LSPDV NCGPA TP G MDCCPPKIN+IIDF
Sbjct: 61  DVLFGLGGLYGVTTLGSNKFALAADDDLSLSPDVQNCGPADTPVGPMDCCPPKINKIIDF 120

Query: 121 TPPIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQ 180
           T PI PKLHVRPA+Q +TPKQL DF+RGIEI+KNLK DDPRNFYQQAK+HCAYCD AYKQ
Sbjct: 121 TKPINPKLHVRPAVQLLTPKQLADFERGIEIMKNLKPDDPRNFYQQAKIHCAYCDSAYKQ 180

Query: 181 LGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYY 240
           +GS+ QTDFL                    ILRHFIGDP+FA+PFWNWDAPIGMHMPI+Y
Sbjct: 181 IGSE-QTDFL--------------------ILRHFIGDPDFAIPFWNWDAPIGMHMPIHY 240

Query: 241 NHNPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFLG 300
           N NPNS LYD NR+P HKAPK+LDLNYDG+TENPPY+VVDCNLRWMNKQMSVKT  +FLG
Sbjct: 241 NGNPNSPLYDANRNPLHKAPKVLDLNYDGSTENPPYDVVDCNLRWMNKQMSVKTPTRFLG 300

Query: 301 KVIKLGETPVYDDAGIIEKSPHNNVHDWVGD-PNNPHREDMGVFYSAARDPIFYAHHANV 360
           KV KLGETP  D  G IE SPHNNVH+WVG+ P  P+ EDMG FY+AARDPIFYAHHANV
Sbjct: 301 KVCKLGETPTLDGMGTIESSPHNNVHNWVGNYPITPNGEDMGAFYAAARDPIFYAHHANV 360

Query: 361 DRMWNIWQKMNNDRRHY-SDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDI 420
           DR+W IW KMN+ R  Y    DWLN +FVFYDE +N VRIKVSDCLDSENLGYVY+DV+I
Sbjct: 361 DRVWYIWNKMNSGRIPYIYRQDWLNSTFVFYDEHRNAVRIKVSDCLDSENLGYVYQDVEI 420

Query: 421 PWLHTKPIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEEVLVIEDVEF 480
           PWL+TK +PR KRS    EPP INFPQ L+SAITT VER S  E +ENEEEVLVIED+E+
Sbjct: 421 PWLNTKQVPRTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEPQENEEEVLVIEDIEY 480

Query: 481 DPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEMLGL 540
           DP+  LHFNV+V+VVD+GDDVCPGNVEF+GSFKNIPHG +H+ K+SLRFVLTE IE LGL
Sbjct: 481 DPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKSSLRFVLTEAIEALGL 540

Query: 541 KDAQKLAVKIDPKTGGDQISIGGIKI 558
            DA+KLAV+IDPK  GDQI +GGIKI
Sbjct: 541 HDARKLAVRIDPKLNGDQIKVGGIKI 545

BLAST of Lsi02G007320 vs. NCBI nr
Match: KAA0056962.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 680.6 bits (1755), Expect = 1.1e-191
Identity = 336/564 (59.57%), Postives = 419/564 (74.29%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK---NIPQNKRH-CVSYISCRASNGENYQNPQAGGDSVSKFDRR 60
           M SLSSLL LS Y   K   +I  NKRH  V+Y+SC+A++  N      G DSVSKFDRR
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAASSNN------GEDSVSKFDRR 60

Query: 61  DVLFGLGGLYGVTTLNTNKFALAESLLSPDVHNCGPAKTPAGEMDCCPPKINRIIDFTPP 120
           +VLFGLGGLYGV T   +  A   + L+PDV NC  A+TP G  +CCPP+I +II F PP
Sbjct: 61  EVLFGLGGLYGVVTATGSVLA---APLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFVPP 120

Query: 121 IIPKLHV-RPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQLG 180
            I + +V RPA   +   Q+E+ +RGI+ +K L  DDP +F  QAK+HCAYCD+AY QLG
Sbjct: 121 KIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLG 180

Query: 181 SDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYYNH 240
           S  +T F VHSNALFFPFHRAYLYFFERILRH+IG+P+FA+PFWNWD+P GMHMP +YN 
Sbjct: 181 SITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYN- 240

Query: 241 NPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFLGKV 300
            P+SSLYD  RDPRH   K++DLNY+G TE+  ++VVDCNLRWMN +M V++ R FLG+V
Sbjct: 241 RPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRV 300

Query: 301 IKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIFYAHHANVDRM 360
           +K GETP   D G IE+SPHNN+HDWVG    PH EDMG FYSAA DP+FYAHH N+DR+
Sbjct: 301 LKAGETPKPSDMGTIEQSPHNNIHDWVG-TKTPHFEDMGSFYSAALDPLFYAHHTNIDRL 360

Query: 361 WNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDIPW-- 420
           WNIW+ ++ + + ++DPDWLN SFVFYDE KN VRI+VSDCLD+E LGYVY++V +PW  
Sbjct: 361 WNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLL 420

Query: 421 LHTKPIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEEVLVIEDVEFDP 480
           L+  P P      +  EPP INFPQ LNS ITTMV+R       E  + VLV+EDVE+DP
Sbjct: 421 LNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDVVLVLEDVEYDP 480

Query: 481 SLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEMLGLKD 540
           + R+HF+V+VNVVD+  D C   +EFVGSFKNIPHGL H +KTSLRFV+TEVI+ LGL+ 
Sbjct: 481 TTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLER 540

Query: 541 AQKLAVKIDPKTGGDQISIGGIKI 558
            + LAVKI+P  GG+ +++GGIKI
Sbjct: 541 DKNLAVKIEPMVGGEGVTLGGIKI 553

BLAST of Lsi02G007320 vs. NCBI nr
Match: XP_004146641.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical protein Csa_013698 [Cucumis sativus])

HSP 1 Score: 679.9 bits (1753), Expect = 1.9e-191
Identity = 335/564 (59.40%), Postives = 417/564 (73.94%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK---NIPQNKRH-CVSYISCRASNGENYQNPQAGGDSVSKFDRR 60
           M SLSSLL LSSY   K   +I  NKRH  V+Y+SC+A++         G DSVSKFDRR
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNN-------GEDSVSKFDRR 60

Query: 61  DVLFGLGGLYGVTTLNTNKFALAESLLSPDVHNCGPAKTPAGEMDCCPPKINRIIDFTPP 120
           +VLFGLGGLYGV T  ++  A   + L+PD+ NC  AKTP G  DCCPP+I +II F PP
Sbjct: 61  EVLFGLGGLYGVVTATSSVLA---APLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPP 120

Query: 121 IIPKLHV-RPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYKQLG 180
            I + +V RPA   +   Q+E+ + GI+ +K L   DP +F  QAK+HCAYCD+ Y QLG
Sbjct: 121 KIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLG 180

Query: 181 SDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIYYNH 240
           S  +T F VHSNALFFPFHRAYLYFFERILRH+IG+PNFA+PFWNWD+P GM+MP +YN 
Sbjct: 181 SITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYN- 240

Query: 241 NPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFLGKV 300
            P+SSLYD  RDPRH   K++ LNY+G TE+  ++VVDCNLRWMN +M V++ R FLG+V
Sbjct: 241 RPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRV 300

Query: 301 IKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPIFYAHHANVDRM 360
           +K GETP   D G IE+SPHNN+HDW+G    PH EDMG FYSAA DP+FYAHHAN+DR+
Sbjct: 301 LKAGETPNPTDMGTIEQSPHNNIHDWLG-TKTPHFEDMGSFYSAALDPLFYAHHANIDRL 360

Query: 361 WNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDIPWLH 420
           WNIW+ ++ + + + DPDWLN SFVFYDE KN V IKVSDCLD+E LGYVY++V +PWLH
Sbjct: 361 WNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLH 420

Query: 421 TK--PIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEEVLVIEDVEFDP 480
               P P      + +EPP +NFPQILNS ITTMV+R       E  +EVLV+EDVE+DP
Sbjct: 421 MNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDP 480

Query: 481 SLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEMLGLKD 540
           + R+HF+V+VNVVD   D C   +EFVGSFKNIPHGL H +KTSLRFV+TEVI+ LGL+ 
Sbjct: 481 TTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLER 540

Query: 541 AQKLAVKIDPKTGGDQISIGGIKI 558
            + LAVKI+P  GG+ +++GGIKI
Sbjct: 541 DKNLAVKIEPMVGGEGVTVGGIKI 552

BLAST of Lsi02G007320 vs. NCBI nr
Match: KAA0056965.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26391.1 polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26393.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 658.7 bits (1698), Expect = 4.5e-185
Identity = 345/570 (60.53%), Postives = 418/570 (73.33%), Query Frame = 0

Query: 1   MTSLSSLLSLSSYFPCK----NIPQNKRHCV-SYISCRASNGENYQNPQAGGDSVSKFDR 60
           M SLSSLLS+S Y  CK     IP NKR  V +++SC+ASN         G DSV+KFDR
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNN--------GEDSVNKFDR 60

Query: 61  RDVLFGLGGLYGVTTLNTNKFALAES-LLSPDVHNCGPAKTPA--GEMDCCPPKINRIID 120
           RD+LFGLGGLYGV TL+    ALA+   LSPDVHNC P K  A   +MDCCPPKI  II+
Sbjct: 61  RDMLFGLGGLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIE 120

Query: 121 FTPPIIPKLHVRPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHCAYCDHAYK 180
           FTPP++P   VRPA+  +TP+Q+ D ++GI+++K L  DDPR+F  QAKVHCAYCD AYK
Sbjct: 121 FTPPVVPTPRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYK 180

Query: 181 QLGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAPIGMHMPIY 240
           Q+  D   DF VH NALFFPFHRAYLYFFERIL H +  P+FA+PFWNWDAP GM MP  
Sbjct: 181 QM--DSTMDFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKI 240

Query: 241 YNHNPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMSVKTNRKFL 300
           Y   P+SSLYDENRD  H+ PK+L+LNY G     P  +VD NLRWMN QMSV+T ++FL
Sbjct: 241 YT-KPSSSLYDENRDKCHQPPKLLNLNYGGKEVKDP-EIVDFNLRWMNDQMSVETAKEFL 300

Query: 301 GKVIKLGETPVYDDAGIIEKSPHNNVHDWVGDPN-NPHREDMGVFYSAARDPIFYAHHAN 360
           GKVIK G  P  + +G IE SPHNN+H WVGDP    H EDMG FYSA RDP+FYAHHAN
Sbjct: 301 GKVIKRGNGP-ENGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHAN 360

Query: 361 VDRMWNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGYVYEDVDI 420
           VDRMWNIWQKM +  R ++D DWLN SFV YDE +  VRIKVS CLDS+ LGYVY+DV I
Sbjct: 361 VDRMWNIWQKMKDRPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPI 420

Query: 421 PWLHTKPIPRIKRSKDVAE-PPTINFPQILNSAITTMVERQ--SSGELKENEEEVLVIED 480
           PW  T PI R K+ + +   PPTI+FPQIL+S ITTMV+R   +S E + N+ EVLV+ED
Sbjct: 421 PWKDTPPIRRPKQERSLGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLED 480

Query: 481 VEFDPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVLTEVIEM 540
           VE+DP+L LHF+V++NV D+G+DV  G++EFVG+F N+PHG     KTSL FV+T+ I+ 
Sbjct: 481 VEYDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQ 540

Query: 541 LGLKDAQKLAVKIDPK-TGGDQISIGGIKI 558
           LGL+  +K+AVKI P   GGD+I IGGIKI
Sbjct: 541 LGLEGDKKVAVKIVPNVNGGDRIKIGGIKI 557

BLAST of Lsi02G007320 vs. NCBI nr
Match: XP_008446626.1 (PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo])

HSP 1 Score: 656.4 bits (1692), Expect = 2.2e-184
Identity = 314/515 (60.97%), Postives = 391/515 (75.92%), Query Frame = 0

Query: 46  GGDSVSKFDRRDVLFGLGGLYGVTTLNTNKFALAESLLSPDVHNCGPAKTPAGEMDCCPP 105
           G DSVSKFDRR+VLFGLGGLYGV T   +  A   + L+PDV NC  A+TP G  +CCPP
Sbjct: 209 GEDSVSKFDRREVLFGLGGLYGVVTATGSVLA---APLTPDVFNCDMAQTPGGIKNCCPP 268

Query: 106 KINRIIDFTPPIIPKLHV-RPALQFVTPKQLEDFQRGIEIIKNLKLDDPRNFYQQAKVHC 165
           +I +II F PP I + +V RPA   +   Q+E+ +RGI+ +K L  DDP +F  QAK+HC
Sbjct: 269 RIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHC 328

Query: 166 AYCDHAYKQLGSDPQTDFLVHSNALFFPFHRAYLYFFERILRHFIGDPNFALPFWNWDAP 225
           AYCD+AY QLGS  +T F VHSNALFFPFHRAYLYFFERILRH+IG+P+FA+PFWNWD+P
Sbjct: 329 AYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSP 388

Query: 226 IGMHMPIYYNHNPNSSLYDENRDPRHKAPKILDLNYDGTTENPPYNVVDCNLRWMNKQMS 285
            GMHMP +YN  P+SSLYD  RDPRH   K++DLNY+G TE+  ++VVDCNLRWMN +M 
Sbjct: 389 PGMHMPEFYN-RPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMK 448

Query: 286 VKTNRKFLGKVIKLGETPVYDDAGIIEKSPHNNVHDWVGDPNNPHREDMGVFYSAARDPI 345
           V++ R FLG+V+K GETP   D G IE+SPHNN+HDWVG    PH EDMG FYSAA DP+
Sbjct: 449 VQSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIHDWVG-TKTPHFEDMGSFYSAALDPL 508

Query: 346 FYAHHANVDRMWNIWQKMNNDRRHYSDPDWLNFSFVFYDERKNVVRIKVSDCLDSENLGY 405
           FYAHH N+DR+WNIW+ ++ + + ++DPDWLN SFVFYDE KN VRI+VSDCLD+E LGY
Sbjct: 509 FYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGY 568

Query: 406 VYEDVDIPW--LHTKPIPRIKRSKDVAEPPTINFPQILNSAITTMVERQSSGELKENEEE 465
           VY++V +PW  L+  P P      +  EPP INFPQ LNS ITTMV+R       E  + 
Sbjct: 569 VYQEVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDV 628

Query: 466 VLVIEDVEFDPSLRLHFNVVVNVVDDGDDVCPGNVEFVGSFKNIPHGLQHQEKTSLRFVL 525
           VLV+EDVE+DP+ R+HF+V+VNVVD+  D C   +EFVGSFKNIPHGL H +KTSLRFV+
Sbjct: 629 VLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVI 688

Query: 526 TEVIEMLGLKDAQKLAVKIDPKTGGDQISIGGIKI 558
           TEVI+ LGL+  + LAVKI+P  GG+ +++GGIKI
Sbjct: 689 TEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKI 718