Lsi02G007260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G007260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	polyphenol oxidase, chloroplastic-like
Location	chr02: 6552482 .. 6560067 (+)
RNA-Seq Expression	Lsi02G007260
Synteny	Lsi02G007260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTTTTAAAAAAAAGAAAAAAAAAGACAAAAAGACAAAACAAACAAACATAAAGAATGACTTTGTAGATACAAATTCTCAAAATATATAACCATCATTTTGTGTTCGCAAGTGTTAGTGTCGTGTGATGATTTTTTTTTTTAATATTAGACTTAGTCGTCAAAATTTGAATAAAAAGTAGGTATATTGATGTAATTCTATGAATTACAAAATTGATTCTGTCTTATGCATGATATATATGTTTCTTTTTTCTTTTGAGCTCAACAACATTTAGAGTTGAGGAAAATTCAAATTTTTCTAATTTCTTGAATTTCATCAAAGAGATAAGTATAGAAGGAGAGATGATGGACATTTTCTTTAGAGAGAGACTTAAATCTCTCTTCAAATGAATTTTTTTAATGAATCCAAAAAGAAATTATCAATTCTTAATGAATTTATTAATATGCAAGCTCAAGGTTATTAAATTTTATCATAGGTCAAAGCGAGCATAGCTATTTGACATATGATGTGTGTTAGATATTTGTTGATCGGCCCTATATTCTTTTTAAGGAAGCTTTTGGTGGTTTTTTTTTATCATTGTCACAAATGTTGTCTTAATATTCTTCTCTGGAAAATTTTTACAGGTTTATTTTGTCAACTATTTTTCTTGTTTGAGGCATAGCTTATTATTTCTGTTTGGAAAGATTGTTTCCTTTCTTGGCCACGGTTTTTAAAAGGAGAATAATGGTGTTCATTTAGGGTTGTCGCATTTGATTATTGTGTGAAGAGTGTTTCTGTTCTTGGTGACCGATTTGTGCAACATCGGAGTTCTCGATCTTTATTCACTGTATTTCGTTGTGCTACTTCATTGATTGAAACATTTAAGAGCTTGAAGATAATTTTTGTCTTAGAGAGAGCCTAAGGCACTACTGTTGAGAAGGCCGGATTCTTGGTCTTAGGGAGAGCCTAAGACTTCATTCTTTGGAGAAGGAGTTCTCCATCTTATAGAGAACATCTAAGATTTGTCAATGAAGGGAGTTCACTAAGTTAGAGGGAAGATAACATTGGTGGAGGGAGTCTCACACACTATCGAAAGCCGAAGTGTTTAACAATAATATTAACATACTTAGGGAGAGTCTAAGTTATTTCGAGGAGTCTCACATTTAGGGGGGCCTAGGTGATCTGTGAGTTGGGCTCCTACGTGAGTCACTGTAGTAGTCGTTGAATTACAGATAAGTGCTACATTGTAATTGCTTGACTCTTTAATATTAGTGGATTATCTTTCCTAGGCATACTGCCCCCTAAACGTAGGTAGATTTTATCAAATGGAGTTACCAACTTCTGTGTGCCTTTACTTTCTTGCTGTTAGTTTTGTTTGTTGTTATAGTGCTTATTTGTGCTTTCTGTTTAACATTCGTGTTTCGAGTGACGAGTCATCTTATAGTTTTTCAATATGTGTTAGTGACCAAGAGGCATGTGGTTCAAATACCCCACCCCTATTGTATTAGAAAAAACTTTTAACCATATCAAAGGTTTTATTTTAGGAAAATTTTTACCGATAGAAAAACTGTCAAACTATTTACAGAAAATAGCAAGAAAAACATTGATAGACATTGATAGACTTCTATCAGCATCTATTAATCTATCACTGATAGACCGTGATAGACTTGTATTAACGTCTATCACACCTATCTAAAAATTTTGCTATTTTGTGTAAACAGTTTCCTTTATTTTTTTATTTTTAAAAATTATCCTTTATTTTACTCTTTTTTATTTTAAAAAAAGTAATGACTATTAATAAATGTCATAAATGTGTAACATTTTAAAACTGTCATTGTATAAAAATCTGAAGGTGCTCCATTTTTTCCCGCCAAAAGTATGAATTTTATGACATTTTTTCTCTCGCCACCCTTAATTTAGCTATACTCTTGTATCATGAAAACTTAGATTCGTTTTAGTACCTATTCTTTTTTGTTATACTTCTAATTTAGTTTTTAATATTTTAATATTTTCATTATAATCTTACTCTTAATGTACACTATATATAAAATGGGCTATGAGGAGAGAATCTTTACGTCTCTAATTTTTACTTATTTTTTAATAATTTTAATTTTGTTCTTCATTCCAGCTACTCGAATCGAAGTGGAAAACAAATTGATAAGAAGGTATATTTTCCACTGATTTGACTACTAGACTATATACACAAAGGTACTAGCACATACGCATGTGATTTACTTTTAAAAGAAAAATTTTCAATGGAATTGATATGATTTTGCATCTTATCAAAGGAAGGAAATTGAAGTATTTAAAGAAAATGGAGAGAAATTTCCTCAACGGTCAAATAATCTAAAGAAGGAAAAGAATAAGTCATGAGCTGAATTTGAGATTAGTATCCAGCTTGATGTTGTATTGATTATGCTTGATGAGTCATGAGAGAATACAATATGTTGCTACCATTATTGTGATTATGGTCAAATTTCTCAAATTTATGGCTTATTTTCTTCCTCTTCGTAATTTCTCAAACTGGAGGGAAGGATAATCCATGTTAGCTCAGCTTTTGCATTTTCCATAGATCTATGCTCTGTTTCTTAATTAGGATGGGACGTTGTTGGTTGTTTCTTTGAAGACCATGAGATTAAGTTTGCTCTAAGATAGGACACAATACTTCGAGATGCTTCTCTTAGTGGTGGGACATCCATCCCAATCTGCATCACAAAATGCATGTAAGTTTAGCGAGCTATTTTTAAAGAAAGAAATATTGTGATTGAGAGTCATCTTTTTAAGATATCTGAGTATTCTTTTGACTTCCCTTAGATTCTTTCCTTGTGGTTATTGAAGATATTGACGAACTTTGTTTACAACATAAGTTATGTTTGGCCTTTTTAGAGTTAAATAATAGAGGGGATACCACTATGCGTCGGTAGAATGTAGGATATATTATTACATCATCCATAAGAGAGCCACTAGGGTTTATAGTCCTTGGTGTGGATATGGGTGATATATTCATCATTTCTGACTTCAAAAGTGTTGGAGTTCTAGATTGGCTATATTTTGTTTAAGAATATTTGGGATGTTGTTGCTCTATTTTGGAGGTTGTCTTAGAAAATATCCCATTGGATGTTTCTAAAAATGTTGATCATATAAGATTATGTTTTTTCTTTTTTGGAATATACTGTGTCTTAAGTATCTCTGGATATTTTCCTTTCTTATTGTCGTGCTGATTTTGTTTTGGGGATGCTTTCCTTGTTCCGTTGCAAGTATTTAAGAGGAAGATAGTGTCTTCCTAGGTTGCTGTTTTGCAATATAAGAAAATACATTGTTTTTTATCTACGGTGGCTGAACTGCTTTGTTCTCTGTGTTTCAAAACTCTGTAGAGCAAAAAATGTAGGCGGTGCTGGGACGACTGTATTCTATATCTTGAAGTTTGAACTGATCTTGAGTAGATTGTACTTCCTTAGGGGACTTGACTGCTAGTTTTACTGCAAAAATATGGCTTGTGTTGTATGGTTTAATTAATGTTTTATGTTTGTATTGCACGAAGAATATTACCTATTGCATCAACCCAAGTATAAATCAATACAATAGCAAGTAGTACTTCGAGTATTCCAAGGTCGATTTCATAGGGATACTTGATGATTGAAGAATGCTAAATCTATTTCACAAGGCTTTCTTCTATGTTGCGTAAAAAAGTGATTTACAAATAGTGATGGTTTGAGTGTTTTAATGCTACAAATGTGCATAATTGACTATTAGACTCCTTATACCTTCACAGCTTCAACATTAATCGGAATAGCCTTCAAATCACAATGCATTCTTTAAGTGAGAAAACCTATTATACCCATCTCCCAATGTAGTATAGAACATCAAATTAGTTGGAGATCATCTGTGCTAAATCTATGTGTTTACTACTGTTTTCTCTATAGCTTATGAGTGTCTATATTATTCCTAATAAAGACTAGTGTTTCATATCTACAGTATTAATTACTGAAAACCAGCGTAATGTTTATACTCTTTCAGCTGCTTTAAATATTTCCACACACTTACTTTTTCTAAAGATTCATGTGATACGATTTTACCTCAATTAGTCAGATAGAGAAAAATACTTTCTGAGTTAAAAAAAATTTTTAAAGCAAAAAGATGCCAATTGAGTTTTAAGATATATTCAATCAATACTGATAATTTGCTACAACATAAGGAGATGATTCTAGTCAAGCCTTTACACTAGAGAATTAGCCACAAATAATCATAATGAAAATAATTTCAACAAAAAAATTTCTAGAAGATAAAAGTTCAGCGAAAACTAGATGCGCAGCAAATGCAAAAAGTTGCAGCTTTGAATTCTAGCTGGGAAATCATCCAACGATCTCCACGGATCTTCTCGAATTATTGTTCCTCTTAGATCTTTTATCCTTGTTTTTTGGCTTCCTCACTCCCCTTCTGGAAGAGAACTTGAGTCTATATATATAGAGGAGATGTGGGACCATAGATGGATGCTGAAGATTTTACCAAACTGATGTGAGAGAACTTTCAAGATAATGTTGGTTCAATGGATGAAGACGTACATCTTCCTGTGCTGCAGCATTACAGTTTTACTTCAATGATATAGCATGACAGTGATGTGACAACTTCATGCACTTCCCCGCTTCTTACTGATGTGACATGTTTAGAGGATAAACTGATATTGTAGGGTAATGATTGTCAATTTGTCGGTTCAAGTCATCAAAAGCAGTTGATTTCTGGAGTATTTGATCATTGACATCCTTGATTCCATCAACACATATTTAGTATCCAAGGGAAATAAATCTTCATCAAATATAACATGTCTAGATATTTAGACTTTATTAGTTTTAGAATCAAAGCATCTATACCCTTTATTGGAATGAGCTATATCATACAAATACACAAGGGTACGTTTTCTTAGCGAACTTGTCGTTAGATTGATATTTCAAGTATGGATAGCATCTACACTTGAACACTTTTAGATTTTTGTAATTTTCATTTTTGTGAAATAACTTTTCAAAGGGAGATTGCATGTTCAAGGATGATGAAGGCATGATATTTATGCAAAAAACCGTGAAAAAAAACTATCTACCCAATACCAATCCATTTTCTACTTTCTTTCGGCTACTCTATTTTGTTGAAGAGTACGAAGGCATGACATTTGATGGTGTCTTCCATTTTGGTCGAAGAATTGTTGAGTCTCAAAGAGGTGAATTCTCCTCCACCATTACTTTGAAATTTGACATAACAATCATATAAATAAGATTTTCTTTTAAGAGGAAAAAGCCAAGTAAATCGTTGGCAAGATAGACAGGTTGACTTTTTGGCCCTTAATATCAAATTAATTAATTTTAATTAATTAATTAATTAATGTTTTGATGATAACAAACCTGCTTTTATTTATTAACAAGTTGAGTGTTTTGAAGTGTCCATTGTGTAGAGCTTGGAACAACGGTTCCAACGCAATTTGAATCACTCAAATCGGAGTTCAAACGAAGAAGATAGGGCTGGAACAAGCTTAACGGAAAAATACCCGATGTCGATGACGTGGCGGCCAAATTATCAAAGTGGACCTACTAGAGGATGACAAGTGGAGAAATAGATAAGTGACACATGGAGATTTTGATTTTTGTTGGTTTTAAAAAGAATGGTTTTATAAAGAAAATGAATTTAATATTATTATATGTGTGTGTTTAACGGCTAGTTGCCTTTAAAAGAAAATGAATTTTATATTATTTTATGTGTGTGTCTAACGGCTAGTTGAGTTTAAATCTCAACCATTCAATTTTCTTTTACCAACATCTAATGGTACAAATTTAATGTAGTTTCCAACAACTTTTCTCTCTTTTTAAACACTCCATCTTCTCCAAATTTTAAGAACTTTTATTATTTTTCAATCCTCCATAATTCATTAAGCTTCCATTGTTGCTCTCTCTCCAAGCTTCAATTTTATTCTTTTCATCTAATTCTTGAGAGAGATTTATTGTATTGTGAGAATTGATTTATTGAGAGCTTAAATTTGTACATTTACTCTTTGAGAGAGTTTTCCTCATCTTATTCTTCATTTCCAAAATTCATTGTAAGGGTTGCTCTTGATCCCTAAAAAGAGTGGGTGTAACGGTTTGATCCTCAACTGTGGAAAGGATCAGGTTTGTTCTTGCGCCCGAAAAAGGAACATTGTAGCGGTTCGACTCTAAGCCGTGGAAAGAGTCAAGGTTTCTAGTGACATACCCAAGAGGAGCTTGGGAAGTGGATGTAGGCCAGTTGTGCCGAACCACTATAAATTTCTAGTGTCATTTTCTCTATCCCTCTCCTATTTAATTTTGCAATTAATTTGCTATTATAGTTTATTGTTTGAAATTAATTGCAATTTTTATTCCTTGAAATTAATTGGTCATATTTGTTTACTTTGATTGGTAGTTCTTAAATTTGCATATCTTTACCAATCTTATTATTAGAATTAAATAGGGTGCTTCAAAGTATTACTTTTAATTTCTTGATTACTTTGGATTGAATTTATTTGCATAAAATTCTTGTTTAAATTACTTGTTAGTATAAAGTGTATTAATTTGTTTAATTGAGCCCACATTAGTAAAAAGTTTTAATTAACTTAATAAAACCCTATTCACCCTCTCTAAGGTTGCCATACCGGTCCAACAAGACACGCTTATTATTTCATTATTAGTAGAGAAAGGAAGTTTGCAACTTTGCTCAATTGACATTACAAATTGCTGTGTTTTTCTTCCAAACATTTTTCTGTGCTTTGCTAAGTTTGCACCGGCTGATTCGTCATTGTAAACAAGCCATAACACCCGCCGACAAGATGGATTGTTGCCCACAGAAAATCGAAGAAATCTTCAACTTCATCCCGCCAGACAACCTAGAACTCCGCATCTGACCTGCCGCCCATTTGATGAATGCAACTGAAATTGCGGAATTAGAAATAGGCATTAAATTAATGAAAGGCCTCAACAAAAACGACCCTTGTAGTTTTTCAAATCAAGTTAAAGTTCATTGTGCTTATTGCGATCATGCCTATAAACAATTAAGGTCTATTCCAAAAACTGATTTTCTAGTTCATCAAAGTTTCTAATTGTTTGGACACTAAAAGGCTGGATATATTTACCAAGACGTGGAGATTCCATGGTTGGACACCAAGAAAATCCCAAGTCCAAATCCGTGGATAAGAACTAAAGAATCTGAGCCTCCAATCATCAACTTCCCACAGATTATGGATTCGGATATAACTACAATGGTAGACAGGCCGGAAGTACCAAGGACCGGAGAGGAAGATGAGGTATTAGTGATTGAAGATGTAGAGTATGATCCAACTACACGAGTGCATTTTGATGTGCTTGTTAATGTTGTTGATGAGGGGCAGAACAAGTGCGCGGCGTTTCTTGAGTTTGTGGGGAGCTTTAAAAACATCTCGCACGGTCTTGAGCATCATAACAAAACCTCTTTGCTCTTTGTGATAACTGAGGTGATTGAAGGGTTGCGTTTGGAGGGTGATAAGAATTTGGCTGTCAGAATTGAACCCGAAGTTTGTGGAAAAAGTGTCACCATTGGAGGAATCAAGATTCAGCTTCAGCGTGTGGAATAA

mRNA sequence

ATGGCTGCTGGATATATTTACCAAGACGTGGAGATTCCATGGTTGGACACCAAGAAAATCCCAAGTCCAAATCCGTGGATAAGAACTAAAGAATCTGAGCCTCCAATCATCAACTTCCCACAGATTATGGATTCGGATATAACTACAATGGTAGACAGGCCGGAAGTACCAAGGACCGGAGAGGAAGATGAGGTATTAGTGATTGAAGATGTAGAGTATGATCCAACTACACGAGTGCATTTTGATGTGCTTGTTAATGTTGTTGATGAGGGGCAGAACAAGTGCGCGGCGTTTCTTGAGTTTGTGGGGAGCTTTAAAAACATCTCGCACGGTCTTGAGCATCATAACAAAACCTCTTTGCTCTTTGTGATAACTGAGGTGATTGAAGGGTTGCGTTTGGAGGGTGATAAGAATTTGGCTGTCAGAATTGAACCCGAAGTTTGTGGAAAAAGTGTCACCATTGGAGGAATCAAGATTCAGCTTCAGCGTGTGGAATAA

Coding sequence (CDS)

ATGGCTGCTGGATATATTTACCAAGACGTGGAGATTCCATGGTTGGACACCAAGAAAATCCCAAGTCCAAATCCGTGGATAAGAACTAAAGAATCTGAGCCTCCAATCATCAACTTCCCACAGATTATGGATTCGGATATAACTACAATGGTAGACAGGCCGGAAGTACCAAGGACCGGAGAGGAAGATGAGGTATTAGTGATTGAAGATGTAGAGTATGATCCAACTACACGAGTGCATTTTGATGTGCTTGTTAATGTTGTTGATGAGGGGCAGAACAAGTGCGCGGCGTTTCTTGAGTTTGTGGGGAGCTTTAAAAACATCTCGCACGGTCTTGAGCATCATAACAAAACCTCTTTGCTCTTTGTGATAACTGAGGTGATTGAAGGGTTGCGTTTGGAGGGTGATAAGAATTTGGCTGTCAGAATTGAACCCGAAGTTTGTGGAAAAAGTGTCACCATTGGAGGAATCAAGATTCAGCTTCAGCGTGTGGAATAA

Protein sequence

MAAGYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGEEDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSLLFVITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRVE

Homology

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BLAST of Lsi02G007260 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.3e-21
Identity = 67/178 (37.64%), Postives = 102/178 (57.30%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIP-SPNPW---IRTKESEPP--IINFPQIMDSDITTMVDRPEVP 63
           GY YQ+V+IPW+++K  P   NPW   +R+K +     I  FP  +DS ++  V RP+  
Sbjct: 406 GYAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKS 465

Query: 64  RT----GEEDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLE 123
           R+     +E+EVLVIE ++++P   + FDV +N  DE  +  A   EF GSF N+ H   
Sbjct: 466 RSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDEDPS-AADQTEFAGSFVNVPHFHR 525

Query: 124 HHNK------TSLLFVITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRVE 166
           H +K      T+L   I+EV++ L ++GD ++ V + P V    +TIGG KI+  R E
Sbjct: 526 HGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIEFVRDE 582

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BLAST of Lsi02G007260 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.3e-18
Identity = 63/184 (34.24%), Postives = 92/184 (50.00%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIP--------------------SPNPWIRTKESEPPIINFPQIM 63
           GY YQDV IPW   K +P                    SP  ++  ++S    + FP ++
Sbjct: 421 GYAYQDVPIPWEKAKPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVL 480

Query: 64  DSDITTMVDRPEVPRT----GEEDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFL 123
           ++ ++ +V RP+  R+     EE+EVLVIE +E+     + FDV +N  DE     A   
Sbjct: 481 NNKVSAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNT 540

Query: 124 EFVGSFKNISHGLE-HHNK---TSLLFVITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIG 160
           EF GSF NI H    H NK   TSL   IT+++E L +EGD N+ V + P+     V I 
Sbjct: 541 EFAGSFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKIN 600

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BLAST of Lsi02G007260 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.1e-16
Identity = 63/182 (34.62%), Postives = 95/182 (52.20%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTK------------ESEPPII---NFPQIMDSDIT 63
           GY+YQDV+IPWL +K  P       +K             S   ++    FP  + S I+
Sbjct: 415 GYVYQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKIS 474

Query: 64  TMVDRPEVPRTG-----EEDEVLVIEDVEYDPTTRVHFDVLVNVVDE---GQNKCAAFLE 123
           T+V RP+  +       +E+E+LVIE +E+D    V FDV VN VD+   G +K     E
Sbjct: 475 TVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDK----TE 534

Query: 124 FVGSFKNISHGLEHHNK--TSLLFVITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIK 161
           F GSF ++ H  +H  K  T L   +T+++E +  E D ++ V + P+    +V IGGIK
Sbjct: 535 FAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKF--GAVKIGGIK 590

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BLAST of Lsi02G007260 vs. ExPASy Swiss-Prot
Match: Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-14
Identity = 58/172 (33.72%), Postives = 92/172 (53.49%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWI-----RTKESEPPIINFPQIMDSDITTMVDRPEVPR 63
           GY+YQDVEIPWL+T+  P  +P +     RT  + P  + FP I+D  +  +V RP+  R
Sbjct: 390 GYVYQDVEIPWLNTRPTPKVSPSLLKKFHRTNTANPRQV-FPAILDRVLKVIVTRPKKTR 449

Query: 64  TGEE----DEVLVIEDVEYD-PTTRVHFDVLVNVVDEGQNKCA-AFLEFVGSFKNISHG- 123
           + +E    +E+LVIE +E +     V FDV +N  ++     +    EF GSF ++ H  
Sbjct: 450 SRKEKDELEEILVIEGIELERDHGHVKFDVYINADEDDLAVISPENAEFAGSFVSLWHKP 509

Query: 124 -LEHHNKTSLLFV-ITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQL 162
                 KT LL + I +++E L  + D  + V + P   G ++ I  +KI+L
Sbjct: 510 IKGKRTKTQLLTLSICDILEDLDADEDDYVLVTLVPRNAGDAIKIHNVKIEL 560

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BLAST of Lsi02G007260 vs. ExPASy Swiss-Prot
Match: Q08307 (Polyphenol oxidase E, chloroplastic OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.5e-11
Identity = 52/169 (30.77%), Postives = 83/169 (49.11%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKI-PSPNPWIRTKESEPPIINFPQI-MDSDITTMVDRPEVPRT-- 63
           G+ Y  +  PW + K I  S +  + T    P    FP   +D  I+  + RP   RT  
Sbjct: 417 GFDYAPMPTPWRNFKPIRKSSSGKVNTASIAPVSKVFPLAKLDRAISFSITRPASSRTTQ 476

Query: 64  --GEEDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNIS--HGLEHH 123
              E++E+L    + YD    V FDV +NV            EF GS+ ++   HG   +
Sbjct: 477 EKNEQEEILTFNKISYDDRNYVRFDVFLNVDKTVNADELDKAEFAGSYTSLPHVHGSNTN 536

Query: 124 NKTSLLF--VITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQ 163
           + TSL F   ITE++E + LE +  +AV + P+  G+ V+I  ++I+L+
Sbjct: 537 HVTSLTFKLAITELLEDIGLEDEDTIAVTLVPKAGGEEVSIESVEIKLE 585

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BLAST of Lsi02G007260 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.7e-66
Identity = 125/160 (78.12%), Postives = 144/160 (90.00%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGEE- 63
           GY+YQ+V +PWL   KIP+PNPWIRT+ESEPPI+NFPQI++SDITTMVDRPE  R+GEE 
Sbjct: 396 GYVYQEVPLPWLHMNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV 455

Query: 64  DEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSLLF 123
           DEVLV+EDVEYDPTTRVHFDVLVNVVD+ Q++CAAFLEFVGSFKNI HGL HH+KTSL F
Sbjct: 456 DEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRF 515

Query: 124 VITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQ 163
           VITEVI+GL LE DKNLAV+IEP V G+ VT+GGIKIQL+
Sbjct: 516 VITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQLE 555

[Close]

BLAST of Lsi02G007260 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.2e-64
Identity = 125/162 (77.16%), Postives = 141/162 (87.04%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGEE- 63
           GY+YQ+V +PWL   KIPSPNPWIRT+E EPPIINFPQ ++SDITTMVDRPE   +GEE 
Sbjct: 397 GYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEV 456

Query: 64  DEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSLLF 123
           D VLV+EDVEYDPTTRVHFDVLVNVVDE Q++CAAFLEFVGSFKNI HGL HH+KTSL F
Sbjct: 457 DVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRF 516

Query: 124 VITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRV 165
           VITEVI+GL LE DKNLAV+IEP V G+ VT+GGIKIQL+ V
Sbjct: 517 VITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 558

[Close]

BLAST of Lsi02G007260 vs. ExPASy TrEMBL
Match: A0A1S3BFJ5 (polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.2e-64
Identity = 125/162 (77.16%), Postives = 141/162 (87.04%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGEE- 63
           GY+YQ+V +PWL   KIPSPNPWIRT+E EPPIINFPQ ++SDITTMVDRPE   +GEE 
Sbjct: 562 GYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEV 621

Query: 64  DEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSLLF 123
           D VLV+EDVEYDPTTRVHFDVLVNVVDE Q++CAAFLEFVGSFKNI HGL HH+KTSL F
Sbjct: 622 DVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRF 681

Query: 124 VITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRV 165
           VITEVI+GL LE DKNLAV+IEP V G+ VT+GGIKIQL+ V
Sbjct: 682 VITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 723

[Close]

BLAST of Lsi02G007260 vs. ExPASy TrEMBL
Match: A0A5A7UPD3 (Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00590 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.1e-43
Identity = 94/167 (56.29%), Postives = 121/167 (72.46%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKES----EPPIINFPQIMDSDITTMVDRPE-VPR 63
           GY+YQDVEIPWL+TK++P      RTK S    EPP+INFPQ + S ITT V+RP  V  
Sbjct: 391 GYVYQDVEIPWLNTKQVP------RTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEP 450

Query: 64  TGEEDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKT 123
              E+EVLVIED+EYDPT  +HF+VLV+VVDEG + C   +EF+GSFKNI HG +H  K+
Sbjct: 451 QENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKS 510

Query: 124 SLLFVITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRVE 166
           SL FV+TE IE L L   + LAVRI+P++ G  + +GGIKIQ++ +E
Sbjct: 511 SLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKVGGIKIQIEPIE 551

[Close]

BLAST of Lsi02G007260 vs. ExPASy TrEMBL
Match: A0A1S3BFK3 (polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489307 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.1e-43
Identity = 94/167 (56.29%), Postives = 121/167 (72.46%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKES----EPPIINFPQIMDSDITTMVDRPE-VPR 63
           GY+YQDVEIPWL+TK++P      RTK S    EPP+INFPQ + S ITT V+RP  V  
Sbjct: 391 GYVYQDVEIPWLNTKQVP------RTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEP 450

Query: 64  TGEEDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKT 123
              E+EVLVIED+EYDPT  +HF+VLV+VVDEG + C   +EF+GSFKNI HG +H  K+
Sbjct: 451 QENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKS 510

Query: 124 SLLFVITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRVE 166
           SL FV+TE IE L L   + LAVRI+P++ G  + +GGIKIQ++ +E
Sbjct: 511 SLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKVGGIKIQIEPIE 551

[Close]

BLAST of Lsi02G007260 vs. NCBI nr
Match: XP_004146641.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical protein Csa_013698 [Cucumis sativus])

HSP 1 Score: 260.0 bits (663), Expect = 1.4e-65
Identity = 125/160 (78.12%), Postives = 144/160 (90.00%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGEE- 63
           GY+YQ+V +PWL   KIP+PNPWIRT+ESEPPI+NFPQI++SDITTMVDRPE  R+GEE 
Sbjct: 396 GYVYQEVPLPWLHMNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV 455

Query: 64  DEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSLLF 123
           DEVLV+EDVEYDPTTRVHFDVLVNVVD+ Q++CAAFLEFVGSFKNI HGL HH+KTSL F
Sbjct: 456 DEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRF 515

Query: 124 VITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQ 163
           VITEVI+GL LE DKNLAV+IEP V G+ VT+GGIKIQL+
Sbjct: 516 VITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQLE 555

[Close]

BLAST of Lsi02G007260 vs. NCBI nr
Match: XP_008446626.1 (PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo])

HSP 1 Score: 253.1 bits (645), Expect = 1.7e-63
Identity = 125/162 (77.16%), Postives = 141/162 (87.04%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGEE- 63
           GY+YQ+V +PWL   KIPSPNPWIRT+E EPPIINFPQ ++SDITTMVDRPE   +GEE 
Sbjct: 562 GYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEV 621

Query: 64  DEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSLLF 123
           D VLV+EDVEYDPTTRVHFDVLVNVVDE Q++CAAFLEFVGSFKNI HGL HH+KTSL F
Sbjct: 622 DVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRF 681

Query: 124 VITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRV 165
           VITEVI+GL LE DKNLAV+IEP V G+ VT+GGIKIQL+ V
Sbjct: 682 VITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 723

[Close]

BLAST of Lsi02G007260 vs. NCBI nr
Match: KAA0056962.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 253.1 bits (645), Expect = 1.7e-63
Identity = 125/162 (77.16%), Postives = 141/162 (87.04%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGEE- 63
           GY+YQ+V +PWL   KIPSPNPWIRT+E EPPIINFPQ ++SDITTMVDRPE   +GEE 
Sbjct: 397 GYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEV 456

Query: 64  DEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSLLF 123
           D VLV+EDVEYDPTTRVHFDVLVNVVDE Q++CAAFLEFVGSFKNI HGL HH+KTSL F
Sbjct: 457 DVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRF 516

Query: 124 VITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRV 165
           VITEVI+GL LE DKNLAV+IEP V G+ VT+GGIKIQL+ V
Sbjct: 517 VITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 558

[Close]

BLAST of Lsi02G007260 vs. NCBI nr
Match: XP_008446638.1 (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056970.1 polyphenol oxidase [Cucumis melo var. makuwa] >TYK26396.1 polyphenol oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 183.7 bits (465), Expect = 1.3e-42
Identity = 94/167 (56.29%), Postives = 121/167 (72.46%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKES----EPPIINFPQIMDSDITTMVDRPE-VPR 63
           GY+YQDVEIPWL+TK++P      RTK S    EPP+INFPQ + S ITT V+RP  V  
Sbjct: 391 GYVYQDVEIPWLNTKQVP------RTKRSTGQPEPPVINFPQTLSSAITTSVERPSGVEP 450

Query: 64  TGEEDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKT 123
              E+EVLVIED+EYDPT  +HF+VLV+VVDEG + C   +EF+GSFKNI HG +H  K+
Sbjct: 451 QENEEEVLVIEDIEYDPTKNLHFNVLVDVVDEGDDVCPGNVEFLGSFKNIPHGSKHEAKS 510

Query: 124 SLLFVITEVIEGLRLEGDKNLAVRIEPEVCGKSVTIGGIKIQLQRVE 166
           SL FV+TE IE L L   + LAVRI+P++ G  + +GGIKIQ++ +E
Sbjct: 511 SLRFVLTEAIEALGLHDARKLAVRIDPKLNGDQIKVGGIKIQIEPIE 551

[Close]

BLAST of Lsi02G007260 vs. NCBI nr
Match: KAA0056963.1 (polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 172.9 bits (437), Expect = 2.2e-39
Identity = 95/166 (57.23%), Postives = 119/166 (71.69%), Query Frame = 0


Query: 4   GYIYQDVEIPWLDTKKIPSPNPWIRTKESEPPIINFPQIMDSDITTMVDRPEVPRTGE-- 63
           GY+YQDV IPW DT  I  P    R+  + PP I+FPQI+ SDITTMVDRPEV    E  
Sbjct: 398 GYVYQDVPIPWKDTPPIRRPKQ-ERSLGNLPPTIDFPQILSSDITTMVDRPEVASPEERI 457

Query: 64  -EDEVLVIEDVEYDPTTRVHFDVLVNVVDEGQNKCAAFLEFVGSFKNISHGLEHHNKTSL 123
            ++EVLV+EDVEYDPT  +HFDVL+NV DEG++  A  +EFVG+F N+ HG   ++KTSL
Sbjct: 458 NKNEVLVLEDVEYDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSL 517

Query: 124 LFVITEVIEGLRLEGDKNLAVRIEPEV-CGKSVTIGGIKIQLQRVE 166
            FVIT+ I+ L LEGDK +AV+I P V  G  + IGGIKIQL+ +E
Sbjct: 518 HFVITKKIQQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQLEDIE 562

The following BLAST results are available for this feature:

BLAST of Lsi02G007260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6UIL3	1.3e-21	37.64	(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S...	[more]
Q06215	1.3e-18	34.24	Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1	[more]
P43309	2.1e-16	34.62	Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1	[more]
Q9FRX6	3.4e-14	33.72	Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1	[more]
Q08307	3.5e-11	30.77	Polyphenol oxidase E, chloroplastic OS=Solanum lycopersicum OX=4081 PE=3 SV=1	[more]

BLAST of Lsi02G007260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUI1	6.7e-66	78.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1	[more]
A0A5A7UM88	8.2e-64	77.16	Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861...	[more]
A0A1S3BFJ5	8.2e-64	77.16	polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300...	[more]
A0A5A7UPD3	6.1e-43	56.29	Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G0...	[more]
A0A1S3BFK3	6.1e-43	56.29	polyphenol oxidase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489307 P...	[more]

BLAST of Lsi02G007260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146641.1	1.4e-65	78.13	polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical pro...	[more]
XP_008446626.1	1.7e-63	77.16	PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo]	[more]
KAA0056962.1	1.7e-63	77.16	polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I...	[more]
XP_008446638.1	1.3e-42	56.29	PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis melo] >KAA0056970.1 p...	[more]
KAA0056963.1	2.2e-39	57.23	polyphenol oxidase I [Cucumis melo var. makuwa]	[more]

BLAST of Lsi02G007260 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022740	Polyphenol oxidase, C-terminal	PFAM	PF12143	PPO1_KFDV	coord: 39..161 e-value: 3.7E-25 score: 88.5
None	No IPR available	PANTHER	PTHR11474	TYROSINASE FAMILY MEMBER	coord: 4..162
None	No IPR available	PANTHER	PTHR11474:SF95	POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKE	coord: 4..162

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G007260.1	Lsi02G007260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004097	catechol oxidase activity