Lsi02G006850 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G006850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Receptor-like serine/threonine-protein kinase
Location	chr02: 6142142 .. 6143142 (-)
RNA-Seq Expression	Lsi02G006850
Synteny	Lsi02G006850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAAGATGAGGATGGGTTTTTGAAGATGGAAATGGTTAAAATTTCAGATTCTCCAGCTTGGATTGTTGCTTCAGAAAATGAGTGCAGAGTCCAGTGTTTGTCAAACTGTTCTTGTTCTGCTTATGCATATAAACAGGGATTGGTTGTATGATATGGAGAAGAGACTTAATTGACGTTCAACAGTTCAAGAATGATGGAGCAGACATTTATGTTCGTGTGGCATATTCAGAAATAGCTGGTATAAAATCATTAATCCCGCACAGAATGACTTTCTAAATGCATCGTGAAAACAAAAAGTACTTAGAAAATCATTTCAAACAGAACCTTTAAATTGCCTCATACATTTTTTGGTATCTTCTATCACAGATGAAAGTGGAATCACCAAAGACATGAAAGTGGTCATTATAGTTTCTGTGATAACAGGAACCTTCATACTTATCAGCTGCATATATTGCTTATGTAAATAGAAAACTAGAGAATCAGGTAAAAAAAACTCCCTTTGAAACTATCTTACTGATTTTGCAAGTATTGAACAGGGAAAAGGAAAAAGAAAAGGGAAAGGCAGATCAAGTTTTTGATGAACACTGGTGATATGAAGCATGATAAAGTGACTGAAGTTAAGCTTCAGGAGCTGCCTCTTTTTGATTTTGAGAAGCTGGCAACTGCAACTAACCACTTTCATTTCAATAACAAGCTTGGTCAGGGTGGGTTTGGGCCAGTGTACAAGGTGGGAATATATAGTTCCCTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTGATTAAGTTGAAGAAATATGAAGGAAATTTGCCTTCATTTTTTTAGGGAAAATTTGTAGATGGACAAGAAATAGCAGTAAAGAGGCTTTCAAAGACCTCTGAACAAGGGCTTGAAGAGTTTACAAATGAAGTAATGGTGATTTCAAAACTACAACATAGAAATCTTGTGCAGCTATTTGGTTGTTGTGTGGAAGGAGAAGAAAGGATGCTAGTGTATTAG

mRNA sequence

ATGGGAAAAGATGAGGATGGGTTTTTGAAGATGGAAATGGTTAAAATTTCAGATTCTCCAGCTTGGATTGTTGCTTCAGAAAATGAGTGCAGAGTCCAGTGTTTGTCAAACTGTTCTTGTTCTGCTTATGCATATAAACAGGGATTGGTTGAACCTTCATACTTATCAGCTGCATATATTGCTTATGTAAATAGAAAACTAGAGAATCAGCATGATAAAGTGACTGAAGTTAAGCTTCAGGAGCTGCCTCTTTTTGATTTTGAGAAGCTGGCAACTGCAACTAACCACTTTCATTTCAATAACAAGCTTGGTCAGGGTGGGTTTGGGCCAGTGTACAAGGGAAAATTTGTAGATGGACAAGAAATAGCAGTAAAGAGGCTTTCAAAGACCTCTGAACAAGGGCTTGAAGAGTTTACAAATGAAGTAATGGTGATTTCAAAACTACAACATAGAAATCTTGTGCAGCTATTTGGTTGTTGTGTGGAAGGAGAAGAAAGGATGCTAGTGTATTAG

Coding sequence (CDS)

ATGGGAAAAGATGAGGATGGGTTTTTGAAGATGGAAATGGTTAAAATTTCAGATTCTCCAGCTTGGATTGTTGCTTCAGAAAATGAGTGCAGAGTCCAGTGTTTGTCAAACTGTTCTTGTTCTGCTTATGCATATAAACAGGGATTGGTTGAACCTTCATACTTATCAGCTGCATATATTGCTTATGTAAATAGAAAACTAGAGAATCAGCATGATAAAGTGACTGAAGTTAAGCTTCAGGAGCTGCCTCTTTTTGATTTTGAGAAGCTGGCAACTGCAACTAACCACTTTCATTTCAATAACAAGCTTGGTCAGGGTGGGTTTGGGCCAGTGTACAAGGGAAAATTTGTAGATGGACAAGAAATAGCAGTAAAGAGGCTTTCAAAGACCTCTGAACAAGGGCTTGAAGAGTTTACAAATGAAGTAATGGTGATTTCAAAACTACAACATAGAAATCTTGTGCAGCTATTTGGTTGTTGTGTGGAAGGAGAAGAAAGGATGCTAGTGTATTAG

Protein sequence

MGKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGLVEPSYLSAAYIAYVNRKLENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDGQEIAVKRLSKTSEQGLEEFTNEVMVISKLQHRNLVQLFGCCVEGEERMLVY

Homology

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BLAST of Lsi02G006850 vs. ExPASy Swiss-Prot
Match: Q9SXB8 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 OS=Arabidopsis thaliana OX=3702 GN=At1g11330 PE=1 SV=3)

HSP 1 Score: 163.3 bits (412), Expect = 2.4e-39
Identity = 97/237 (40.93%), Postives = 122/237 (51.48%), Query Frame = 0


Query: 2   GKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL------------ 61
           G   DGFLK++ +K+  S     ASE  C   CL NCSC+AYAY +G+            
Sbjct: 361 GGKADGFLKLQKMKVPISAERSEASEQVCPKVCLDNCSCTAYAYDRGIGCMLWSGDLVDM 420

Query: 62  ---------------------------------VEPSYLSAAYIAYVNRKLE-------- 121
                                            +    ++A  +    RK +        
Sbjct: 421 QSFLGSGIDLFIRVAHSELKTHSNLAVMIAAPVIGVMLIAAVCVLLACRKYKKRPAPAKD 480

Query: 122 ---------------NQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYK 171
                          +      ++KL+ELPLF+F+ LAT+T+ F   NKLGQGGFGPVYK
Sbjct: 481 RSAELMFKRMEALTSDNESASNQIKLKELPLFEFQVLATSTDSFSLRNKLGQGGFGPVYK 540

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BLAST of Lsi02G006850 vs. ExPASy Swiss-Prot
Match: Q9LPZ9 (G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 OS=Arabidopsis thaliana OX=3702 GN=SD113 PE=1 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 1.3e-37
Identity = 91/236 (38.56%), Postives = 124/236 (52.54%), Query Frame = 0


Query: 3   KDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQG-------------- 62
           +  DGF++++ +K+  +P    A+E +C   CL NCSC+AY++ +G              
Sbjct: 350 RKSDGFVRVQKMKVPHNPQRSGANEQDCPESCLKNCSCTAYSFDRGIGCLLWSGNLMDMQ 409

Query: 63  -------------------------------LVEPSYLSAAYIAYVNRKLENQHDK---- 122
                                          L+  ++L A  +     K+    +K    
Sbjct: 410 EFSGTGVVFYIRLADSEFKKRTNRSIVITVTLLVGAFLFAGTVVLALWKIAKHREKNRNT 469

Query: 123 -------------------VTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 171
                              V + KL+ELPLF+F+ LA ATN+F   NKLGQGGFG VYKG
Sbjct: 470 RLLNERMEALSSNDVGAILVNQYKLKELPLFEFQVLAVATNNFSITNKLGQGGFGAVYKG 529

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BLAST of Lsi02G006850 vs. ExPASy Swiss-Prot
Match: Q9SXB4 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11300 OS=Arabidopsis thaliana OX=3702 GN=At1g11300 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.7e-37
Identity = 97/228 (42.54%), Postives = 120/228 (52.63%), Query Frame = 0


Query: 6   DGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQG---------LVEPSYLS 65
           DGFL++  +K+ D      ASE EC   CL  CSC A A+  G         LV+   LS
Sbjct: 355 DGFLRLRRMKLPDFARRSEASEPECLRTCLQTCSCIAAAHGLGYGCMIWNGSLVDSQELS 414

Query: 66  AA----YIAYVNRKLENQHDKVTEV----------------------------------- 125
           A+    YI   + +++ +  +   +                                   
Sbjct: 415 ASGLDLYIRLAHSEIKTKDKRPILIGTILAGGIFVVAACVLLARRIVMKKRAKKKGRDAE 474

Query: 126 ---------------KLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDGQEI 171
                          KL+ELPLF+F+ LA ATN+F   NKLGQGGFGPVYKGK  +GQEI
Sbjct: 475 QIFERVEALAGGNKGKLKELPLFEFQVLAAATNNFSLRNKLGQGGFGPVYKGKLQEGQEI 534

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BLAST of Lsi02G006850 vs. ExPASy Swiss-Prot
Match: Q9SXB5 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11303 OS=Arabidopsis thaliana OX=3702 GN=At1g11303 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 2.9e-37
Identity = 98/228 (42.98%), Postives = 126/228 (55.26%), Query Frame = 0


Query: 6   DGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQG---------LVEPSYLS 65
           D FLK++ +K+ D      ASE EC + CL +CSC A+A+  G         LV+   LS
Sbjct: 355 DRFLKLQRMKMPDFARRSEASEPECFMTCLQSCSCIAFAHGLGYGCMIWNRSLVDSQVLS 414

Query: 66  AA------YIAYVNRKLENQH--------------------------------DKVTEV- 125
           A+       +A+   K +++                                  K T+  
Sbjct: 415 ASGMDLSIRLAHSEFKTQDRRPILIGTSLAGGIFVVATCVLLARRIVMKKRAKKKGTDAE 474

Query: 126 ---------------KLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDGQEI 171
                          KL+ELPLF+F+ LATAT++F  +NKLGQGGFGPVYKG  ++GQEI
Sbjct: 475 QIFKRVEALAGGSREKLKELPLFEFQVLATATDNFSLSNKLGQGGFGPVYKGMLLEGQEI 534

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BLAST of Lsi02G006850 vs. ExPASy Swiss-Prot
Match: O81832 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 OS=Arabidopsis thaliana OX=3702 GN=At4g27290 PE=3 SV=4)

HSP 1 Score: 148.3 bits (373), Expect = 8.0e-35
Identity = 84/196 (42.86%), Postives = 115/196 (58.67%), Query Frame = 0


Query: 2   GKDEDGFLKMEMVKISDS-PAWIVASE--NECRVQCLSNCSCSAYA-------------- 61
           GK EDGFLK+  +K+ D+  +W   +   NEC+  CL NC+CSAY+              
Sbjct: 343 GKGEDGFLKISKLKLPDTRTSWYDKNMDLNECKKVCLRNCTCSAYSPFDIRDGGKGCILW 402

Query: 62  ----------YKQGLVEPSYLSAAYIAYVNRKLENQHDKVTEVKLQELPLFDFEKLATAT 121
                      + G      L+++ I  + R+      +  E +  ELP  D + ++ AT
Sbjct: 403 FGDLIDIREYNENGQDLYVRLASSEIETLQRESSRVSSRKQEEEDLELPFLDLDTVSEAT 462

Query: 122 NHFHFNNKLGQGGFGPVYKGKFVDGQEIAVKRLSKTSEQGLEEFTNEVMVISKLQHRNLV 171
           + F   NKLGQGGFGPVYKG    GQE+AVKRLS+TS QG+EEF NE+ +I+KLQHRNLV
Sbjct: 463 SGFSAGNKLGQGGFGPVYKGTLACGQEVAVKRLSRTSRQGVEEFKNEIKLIAKLQHRNLV 522

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BLAST of Lsi02G006850 vs. ExPASy TrEMBL
Match: A0A1S4DSE6 (uncharacterized protein LOC103489252 OS=Cucumis melo OX=3656 GN=LOC103489252 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.0e-65
Identity = 139/236 (58.90%), Postives = 153/236 (64.83%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL----------- 60
           MGKD+DGFLK+EMVK+ DS  WIVASEN+CRVQCLSNCSCSAYAYK G+           
Sbjct: 346 MGKDQDGFLKLEMVKVPDSAGWIVASENDCRVQCLSNCSCSAYAYKTGIGCMIWRGDLID 405

Query: 61  ------------VEPSYLSAAYIAYVNRKL------------------------------ 120
                       V   Y   AY + +++ +                              
Sbjct: 406 IQQFKNGGADIYVRVPYSEIAYESGISKDMKVVIIASVVTGTFILICSIYCLWKRKRERE 465

Query: 121 -------------ENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 171
                        + +HDKV +VKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG
Sbjct: 466 RQTKTKFLMNNGDDMKHDKVNQVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 525

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BLAST of Lsi02G006850 vs. ExPASy TrEMBL
Match: A0A5A7UQL5 (Receptor-like serine/threonine-protein kinase SD1-8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001010 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.9e-61
Identity = 131/186 (70.43%), Postives = 141/186 (75.81%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSP--AWIVASENECRVQCLSNCSCSAYAYKQGLVEPSYLSAA 60
           MGKD+DGFLK+EMVK+ DS     ++   +   +Q   N     Y       EPSYLSA 
Sbjct: 346 MGKDQDGFLKLEMVKVPDSAGIGCMIWRGDLIDIQQFKNGGADIY-------EPSYLSAP 405

Query: 61  YIAYVNRKLENQ--------------HDKVTEVKLQELPLFDFEKLATATNHFHFNNKLG 120
           YIAYV+RKLENQ              HDKV +VKLQELPLFDFEKLATATNHFHFNNKLG
Sbjct: 406 YIAYVSRKLENQTKTKFLMNNGDDMKHDKVNQVKLQELPLFDFEKLATATNHFHFNNKLG 465

Query: 121 QGGFGPVYKGKFVDGQEIAVKRLSKTSEQGLEEFTNEVMVISKLQHRNLVQLFGCCVEGE 171
           QGGFGPVYKGK VDGQEIAVKRLSKTS QGLEEFTNEVMVISKLQHRNLVQLFGCCV+GE
Sbjct: 466 QGGFGPVYKGKLVDGQEIAVKRLSKTSGQGLEEFTNEVMVISKLQHRNLVQLFGCCVDGE 524

[Close]

BLAST of Lsi02G006850 vs. ExPASy TrEMBL
Match: A0A5D3DRU5 (Receptor-like serine/threonine-protein kinase SD1-8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00150 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.9e-61
Identity = 131/186 (70.43%), Postives = 141/186 (75.81%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSP--AWIVASENECRVQCLSNCSCSAYAYKQGLVEPSYLSAA 60
           MGKD+DGFLK+EMVK+ DS     ++   +   +Q   N     Y       EPSYLSA 
Sbjct: 346 MGKDQDGFLKLEMVKVPDSAGIGCMIWRGDLIDIQQFKNGGADIY-------EPSYLSAP 405

Query: 61  YIAYVNRKLENQ--------------HDKVTEVKLQELPLFDFEKLATATNHFHFNNKLG 120
           YIAYV+RKLENQ              HDKV +VKLQELPLFDFEKLATATNHFHFNNKLG
Sbjct: 406 YIAYVSRKLENQTKTKFLMNNGDDMKHDKVNQVKLQELPLFDFEKLATATNHFHFNNKLG 465

Query: 121 QGGFGPVYKGKFVDGQEIAVKRLSKTSEQGLEEFTNEVMVISKLQHRNLVQLFGCCVEGE 171
           QGGFGPVYKGK VDGQEIAVKRLSKTS QGLEEFTNEVMVISKLQHRNLVQLFGCCV+GE
Sbjct: 466 QGGFGPVYKGKLVDGQEIAVKRLSKTSGQGLEEFTNEVMVISKLQHRNLVQLFGCCVDGE 524

[Close]

BLAST of Lsi02G006850 vs. ExPASy TrEMBL
Match: A0A6J1EHE3 (Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111434315 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.9e-61
Identity = 136/236 (57.63%), Postives = 151/236 (63.98%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL----------- 60
           MG D+DGFLK+ MVK+ D  AW+VASE++CRVQCL+NCSCSAYAY+ G+           
Sbjct: 346 MGTDQDGFLKVGMVKVPDFAAWVVASEDDCRVQCLANCSCSAYAYRTGIGCMIWRGDLID 405

Query: 61  ------------VEPSY------------LSAAYIAYV---------------------- 120
                       V  +Y            + A  +A V                      
Sbjct: 406 IQEFKNGGADIYVRVAYSDIANESGTTKDMKAVIVASVVAGTFILICSIYCLWKVQMQRQ 465

Query: 121 ----NRKL-----ENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 171
                RK      E +HDKV +VKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG
Sbjct: 466 KRKEQRKFLMNSGEMKHDKVNQVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 525

[Close]

BLAST of Lsi02G006850 vs. ExPASy TrEMBL
Match: A0A6J1CFS5 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111010815 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.2e-59
Identity = 129/226 (57.08%), Postives = 145/226 (64.16%), Query Frame = 0


Query: 5   EDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL--------------- 64
           EDGFLK+EMVK+ D   WIVASE+ECRVQCLSNCSCSAY YK G+               
Sbjct: 350 EDGFLKVEMVKVPDFADWIVASEDECRVQCLSNCSCSAYTYKTGIGCMIWRGDLIDIQNF 409

Query: 65  ------------------------------VEPSYLSAAYI---------------AYVN 124
                                         +  S +S  +I                ++ 
Sbjct: 410 KNFGVDIYVRVAYSDLADESGSTTKDVKAVIIASVISGTFILICCIYCFWKRKSQLKFLF 469

Query: 125 RKLENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDGQEIAV 171
           +  + +HDK+ +VKLQELPLFDFEKL TATNHFHFNNKLG+GGFGPVYKGK VDGQEIAV
Sbjct: 470 KTGDMKHDKMDQVKLQELPLFDFEKLETATNHFHFNNKLGEGGFGPVYKGKLVDGQEIAV 529

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BLAST of Lsi02G006850 vs. NCBI nr
Match: XP_016898911.1 (PREDICTED: uncharacterized protein LOC103489252 [Cucumis melo])

HSP 1 Score: 258.5 bits (659), Expect = 4.2e-65
Identity = 139/236 (58.90%), Postives = 153/236 (64.83%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL----------- 60
           MGKD+DGFLK+EMVK+ DS  WIVASEN+CRVQCLSNCSCSAYAYK G+           
Sbjct: 346 MGKDQDGFLKLEMVKVPDSAGWIVASENDCRVQCLSNCSCSAYAYKTGIGCMIWRGDLID 405

Query: 61  ------------VEPSYLSAAYIAYVNRKL------------------------------ 120
                       V   Y   AY + +++ +                              
Sbjct: 406 IQQFKNGGADIYVRVPYSEIAYESGISKDMKVVIIASVVTGTFILICSIYCLWKRKRERE 465

Query: 121 -------------ENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 171
                        + +HDKV +VKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG
Sbjct: 466 RQTKTKFLMNNGDDMKHDKVNQVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 525

[Close]

BLAST of Lsi02G006850 vs. NCBI nr
Match: XP_031738233.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 [Cucumis sativus] >KAE8652693.1 hypothetical protein Csa_013571 [Cucumis sativus])

HSP 1 Score: 254.6 bits (649), Expect = 6.0e-64
Identity = 139/236 (58.90%), Postives = 153/236 (64.83%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL----------- 60
           +GKD+DGFLK+EMVK+ DS  WIVASEN+CRVQCLSNCSCSAYAYK G+           
Sbjct: 348 IGKDQDGFLKLEMVKVPDSAGWIVASENDCRVQCLSNCSCSAYAYKTGIGCMIWRGDLID 407

Query: 61  ------------VEPSYLSAAYIAYVN---------------------------RKLENQ 120
                       V  +Y   AY + ++                           RK E +
Sbjct: 408 IQQFKNGGADIYVRGAYSEIAYESGISKDVKVVIVASVVTGSFILICCIYCLWKRKRERE 467

Query: 121 ----------------HDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 171
                           HDKV +VKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG
Sbjct: 468 RQTKIKFLMNNGDDMKHDKVNQVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 527

[Close]

BLAST of Lsi02G006850 vs. NCBI nr
Match: XP_038894409.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 isoform X2 [Benincasa hispida])

HSP 1 Score: 253.4 bits (646), Expect = 1.3e-63
Identity = 138/230 (60.00%), Postives = 152/230 (66.09%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL----------- 60
           MGKD+DGFLKMEMVK+ DS AWIV SEN+CRVQCLSNCSCSAYAY+ G+           
Sbjct: 345 MGKDQDGFLKMEMVKVPDSAAWIVTSENDCRVQCLSNCSCSAYAYRTGIGCMIWRGGLID 404

Query: 61  ------------VEPSY------------LSAAYIAYV---------------------- 120
                       V  +Y            L  + IA V                      
Sbjct: 405 IQQFKKDGADINVRVAYSEIADESGITKDLKVSIIASVVTGTLILISCIYCLWKRKRQNK 464

Query: 121 ---NRKLENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDGQ 171
              N  ++ +HDKV EVKLQ+LP+FDF+KLA ATNHFH NNKLGQGGFGPVYKGK VDGQ
Sbjct: 465 FLMNTDVDMKHDKVNEVKLQQLPVFDFDKLAIATNHFHSNNKLGQGGFGPVYKGKLVDGQ 524

[Close]

BLAST of Lsi02G006850 vs. NCBI nr
Match: XP_038894412.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 isoform X3 [Benincasa hispida] >XP_038894413.1 G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 isoform X4 [Benincasa hispida])

HSP 1 Score: 245.4 bits (625), Expect = 3.6e-61
Identity = 136/231 (58.87%), Postives = 151/231 (65.37%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSPAW-IVASENECRVQCLSNCSCSAYAYKQGL---------- 60
           MGKDEDGFLK+ MVK+ DS AW +VASEN+CR+QC SNCSCSAYAYK G+          
Sbjct: 346 MGKDEDGFLKLGMVKVPDSAAWVVVASENDCRIQCASNCSCSAYAYKIGIGCMIWRGDLI 405

Query: 61  -------------VEPSY------------LSAAYIAYV--------------------- 120
                        V  +Y            L  + IA V                     
Sbjct: 406 DIQQFKNDGADIYVRVAYSEIADESGITKDLKVSIIASVVTGTLILISCIYCLWKRKRQN 465

Query: 121 ----NRKLENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDG 171
               N  ++ +HDKV EVKLQ+LP+FDF+KLA ATNHFH NNKLGQGGFGPVYKGK VDG
Sbjct: 466 KFLMNTDVDMKHDKVNEVKLQQLPVFDFDKLAIATNHFHSNNKLGQGGFGPVYKGKLVDG 525

[Close]

BLAST of Lsi02G006850 vs. NCBI nr
Match: XP_038894407.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11300 isoform X1 [Benincasa hispida] >XP_038894408.1 G-type lectin S-receptor-like serine/threonine-protein kinase At1g11300 isoform X1 [Benincasa hispida])

HSP 1 Score: 245.4 bits (625), Expect = 3.6e-61
Identity = 136/231 (58.87%), Postives = 151/231 (65.37%), Query Frame = 0


Query: 1   MGKDEDGFLKMEMVKISDSPAW-IVASENECRVQCLSNCSCSAYAYKQGL---------- 60
           MGKDEDGFLK+ MVK+ DS AW +VASEN+CR+QC SNCSCSAYAYK G+          
Sbjct: 346 MGKDEDGFLKLGMVKVPDSAAWVVVASENDCRIQCASNCSCSAYAYKIGIGCMIWRGDLI 405

Query: 61  -------------VEPSY------------LSAAYIAYV--------------------- 120
                        V  +Y            L  + IA V                     
Sbjct: 406 DIQQFKNDGADIYVRVAYSEIADESGITKDLKVSIIASVVTGTLILISCIYCLWKRKRQN 465

Query: 121 ----NRKLENQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDG 171
               N  ++ +HDKV EVKLQ+LP+FDF+KLA ATNHFH NNKLGQGGFGPVYKGK VDG
Sbjct: 466 KFLMNTDVDMKHDKVNEVKLQQLPVFDFDKLAIATNHFHSNNKLGQGGFGPVYKGKLVDG 525

[Close]

BLAST of Lsi02G006850 vs. TAIR 10
Match: AT1G11330.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 164.9 bits (416), Expect = 5.8e-41
Identity = 98/235 (41.70%), Postives = 123/235 (52.34%), Query Frame = 0


Query: 2   GKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL------------ 61
           G   DGFLK++ +K+  S     ASE  C   CL NCSC+AYAY +G+            
Sbjct: 361 GGKADGFLKLQKMKVPISAERSEASEQVCPKVCLDNCSCTAYAYDRGIGCMLWSGDLVDM 420

Query: 62  ---------------------------------VEPSYLSAAYIAYVNRKLENQHDK--- 121
                                            +    ++A  +    RK + +  K   
Sbjct: 421 QSFLGSGIDLFIRVAHSELKTHSNLAVMIAAPVIGVMLIAAVCVLLACRKYKKRPAKDRS 480

Query: 122 ------------------VTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGK 171
                               ++KL+ELPLF+F+ LAT+T+ F   NKLGQGGFGPVYKGK
Sbjct: 481 AELMFKRMEALTSDNESASNQIKLKELPLFEFQVLATSTDSFSLRNKLGQGGFGPVYKGK 540

[Close]

BLAST of Lsi02G006850 vs. TAIR 10
Match: AT1G11330.2 (S-locus lectin protein kinase family protein )

HSP 1 Score: 163.3 bits (412), Expect = 1.7e-40
Identity = 97/237 (40.93%), Postives = 122/237 (51.48%), Query Frame = 0


Query: 2   GKDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQGL------------ 61
           G   DGFLK++ +K+  S     ASE  C   CL NCSC+AYAY +G+            
Sbjct: 361 GGKADGFLKLQKMKVPISAERSEASEQVCPKVCLDNCSCTAYAYDRGIGCMLWSGDLVDM 420

Query: 62  ---------------------------------VEPSYLSAAYIAYVNRKLE-------- 121
                                            +    ++A  +    RK +        
Sbjct: 421 QSFLGSGIDLFIRVAHSELKTHSNLAVMIAAPVIGVMLIAAVCVLLACRKYKKRPAPAKD 480

Query: 122 ---------------NQHDKVTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYK 171
                          +      ++KL+ELPLF+F+ LAT+T+ F   NKLGQGGFGPVYK
Sbjct: 481 RSAELMFKRMEALTSDNESASNQIKLKELPLFEFQVLATSTDSFSLRNKLGQGGFGPVYK 540

[Close]

BLAST of Lsi02G006850 vs. TAIR 10
Match: AT1G11350.1 (S-domain-1 13 )

HSP 1 Score: 157.5 bits (397), Expect = 9.3e-39
Identity = 91/236 (38.56%), Postives = 124/236 (52.54%), Query Frame = 0


Query: 3   KDEDGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQG-------------- 62
           +  DGF++++ +K+  +P    A+E +C   CL NCSC+AY++ +G              
Sbjct: 350 RKSDGFVRVQKMKVPHNPQRSGANEQDCPESCLKNCSCTAYSFDRGIGCLLWSGNLMDMQ 409

Query: 63  -------------------------------LVEPSYLSAAYIAYVNRKLENQHDK---- 122
                                          L+  ++L A  +     K+    +K    
Sbjct: 410 EFSGTGVVFYIRLADSEFKKRTNRSIVITVTLLVGAFLFAGTVVLALWKIAKHREKNRNT 469

Query: 123 -------------------VTEVKLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKG 171
                              V + KL+ELPLF+F+ LA ATN+F   NKLGQGGFG VYKG
Sbjct: 470 RLLNERMEALSSNDVGAILVNQYKLKELPLFEFQVLAVATNNFSITNKLGQGGFGAVYKG 529

[Close]

BLAST of Lsi02G006850 vs. TAIR 10
Match: AT1G11300.1 (protein serine/threonine kinases;protein kinases;ATP binding;sugar binding;kinases;carbohydrate binding )

HSP 1 Score: 156.4 bits (394), Expect = 2.1e-38
Identity = 98/228 (42.98%), Postives = 126/228 (55.26%), Query Frame = 0


Query: 6    DGFLKMEMVKISDSPAWIVASENECRVQCLSNCSCSAYAYKQG---------LVEPSYLS 65
            D FLK++ +K+ D      ASE EC + CL +CSC A+A+  G         LV+   LS
Sbjct: 1185 DRFLKLQRMKMPDFARRSEASEPECFMTCLQSCSCIAFAHGLGYGCMIWNRSLVDSQVLS 1244

Query: 66   AA------YIAYVNRKLENQH--------------------------------DKVTEV- 125
            A+       +A+   K +++                                  K T+  
Sbjct: 1245 ASGMDLSIRLAHSEFKTQDRRPILIGTSLAGGIFVVATCVLLARRIVMKKRAKKKGTDAE 1304

Query: 126  ---------------KLQELPLFDFEKLATATNHFHFNNKLGQGGFGPVYKGKFVDGQEI 171
                           KL+ELPLF+F+ LATAT++F  +NKLGQGGFGPVYKG  ++GQEI
Sbjct: 1305 QIFKRVEALAGGSREKLKELPLFEFQVLATATDNFSLSNKLGQGGFGPVYKGMLLEGQEI 1364

[Close]

BLAST of Lsi02G006850 vs. TAIR 10
Match: AT4G27290.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 148.3 bits (373), Expect = 5.7e-36
Identity = 84/196 (42.86%), Postives = 115/196 (58.67%), Query Frame = 0


Query: 2   GKDEDGFLKMEMVKISDS-PAWIVASE--NECRVQCLSNCSCSAYA-------------- 61
           GK EDGFLK+  +K+ D+  +W   +   NEC+  CL NC+CSAY+              
Sbjct: 343 GKGEDGFLKISKLKLPDTRTSWYDKNMDLNECKKVCLRNCTCSAYSPFDIRDGGKGCILW 402

Query: 62  ----------YKQGLVEPSYLSAAYIAYVNRKLENQHDKVTEVKLQELPLFDFEKLATAT 121
                      + G      L+++ I  + R+      +  E +  ELP  D + ++ AT
Sbjct: 403 FGDLIDIREYNENGQDLYVRLASSEIETLQRESSRVSSRKQEEEDLELPFLDLDTVSEAT 462

Query: 122 NHFHFNNKLGQGGFGPVYKGKFVDGQEIAVKRLSKTSEQGLEEFTNEVMVISKLQHRNLV 171
           + F   NKLGQGGFGPVYKG    GQE+AVKRLS+TS QG+EEF NE+ +I+KLQHRNLV
Sbjct: 463 SGFSAGNKLGQGGFGPVYKGTLACGQEVAVKRLSRTSRQGVEEFKNEIKLIAKLQHRNLV 522

The following BLAST results are available for this feature:

BLAST of Lsi02G006850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXB8	2.4e-39	40.93	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 OS=Arabi...	[more]
Q9LPZ9	1.3e-37	38.56	G-type lectin S-receptor-like serine/threonine-protein kinase SD1-13 OS=Arabidop...	[more]
Q9SXB4	1.7e-37	42.54	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11300 OS=Arabi...	[more]
Q9SXB5	2.9e-37	42.98	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11303 OS=Arabi...	[more]
O81832	8.0e-35	42.86	G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 OS=Arabi...	[more]

BLAST of Lsi02G006850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DSE6	2.0e-65	58.90	uncharacterized protein LOC103489252 OS=Cucumis melo OX=3656 GN=LOC103489252 PE=...	[more]
A0A5A7UQL5	3.9e-61	70.43	Receptor-like serine/threonine-protein kinase SD1-8 OS=Cucumis melo var. makuwa ...	[more]
A0A5D3DRU5	3.9e-61	70.43	Receptor-like serine/threonine-protein kinase SD1-8 OS=Cucumis melo var. makuwa ...	[more]
A0A6J1EHE3	3.9e-61	57.63	Receptor-like serine/threonine-protein kinase OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1CFS5	2.2e-59	57.08	Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=...	[more]

BLAST of Lsi02G006850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016898911.1	4.2e-65	58.90	PREDICTED: uncharacterized protein LOC103489252 [Cucumis melo]	[more]
XP_031738233.1	6.0e-64	58.90	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 [Cucumis...	[more]
XP_038894409.1	1.3e-63	60.00	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 isoform ...	[more]
XP_038894412.1	3.6e-61	58.87	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330 isoform ...	[more]
XP_038894407.1	3.6e-61	58.87	G-type lectin S-receptor-like serine/threonine-protein kinase At1g11300 isoform ...	[more]

BLAST of Lsi02G006850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11330.1	5.8e-41	41.70	S-locus lectin protein kinase family protein	[more]
AT1G11330.2	1.7e-40	40.93	S-locus lectin protein kinase family protein	[more]
AT1G11350.1	9.3e-39	38.56	S-domain-1 13	[more]
AT1G11300.1	2.1e-38	42.98	protein serine/threonine kinases;protein kinases;ATP binding;sugar binding;kinas...	[more]
AT4G27290.1	5.7e-36	42.86	S-locus lectin protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003609	PAN/Apple domain	PFAM	PF08276	PAN_2	coord: 3..47 e-value: 2.1E-11 score: 43.8
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 57..170 e-value: 1.5E-37 score: 129.8
None	No IPR available	PANTHER	PTHR32444	FAMILY NOT NAMED	coord: 65..170
None	No IPR available	PANTHER	PTHR32444:SF69	G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-13	coord: 4..55 coord: 65..170
None	No IPR available	PANTHER	PTHR32444	FAMILY NOT NAMED	coord: 4..55
None	No IPR available	CDD	cd01098	PAN_AP_plant	coord: 2..48 e-value: 3.01249E-14 score: 62.4538
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 98..169 e-value: 1.5E-13 score: 50.7
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 97..170 score: 13.997621
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 82..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G006850.1	Lsi02G006850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016020	membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity