Lsi02G005370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G005370
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCationic amino acid transporter like
Locationchr02: 4663610 .. 4665373 (-)
RNA-Seq ExpressionLsi02G005370
SyntenyLsi02G005370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGAAGAAGTTGTTCAGAAGCAGAGAAGCTATTGGTGGAGATGGAACAAACAAGATTTCTTACCAGAAGAATCTTTCCAGAGTTGGAGCAATTACGGAACTGCTCTGTCACAGACATGGTTTCGGTTCCTGGACCGTCTTCAAAGCAGATCACTCGATGAAAATGAAATTGGAGAGCTAAGAAAACGAAGTGAGAATGAAATGAAACGTTGCCTTACTTGGTGGGATCTCACTTGGTTTGGATTTGGAGCTGTAATTGGTGCAGGAATCTTCGTCCTCACAGGCCAAGAAGCCAACAAACATGCTGGACCAGCCATTGTTCTGTCTTATGTAGCTTCAGGCATTTCTGCAATGCTCTCTGTTTTTTGCTATACAGAGTTCGCTATTGAAATCCCAGTTGCAGGGGGATCTTTTGCATACTTAAGAATTGAATTGGGGGACTTTGCAGCTTTCATTACTGCAGGCAATATTCTCCTCGAGAGCATTGTCGGTACTGCCGCCGTCGCTCGAGCTTGGACTTCTTACTTTACCTCGCTTTTGGACCGACCCGACAACTCGTTGCGTATCCACACGAATCTCAAAGATGGATTCAATCTTCTCGACCCAATTGCCGTTGCGGTTTTAGCGATTGCTGCGACAATAGCGATGACCAGCACGAGAAAAACTTCATACCTGAATTGGATTGCTTCAGCTGTTAATACAGTTGTGATTCTGTTTGTTATAATTGCAGGTTTTGTACATGCTGATAAATCAAATTTGACTCCTTTTATGCCTTTTGGAGTTAAAGGTGTCTTCCAAGCTGCTGCAATTGTTTACTTTGCTTATGGAGGTTTTGATAACATTGCTACAATGGCTGAGGAAACTAAAAATCCATCAAAAGATATACCTTTGGGATTGTTGGGATCAATGTCGATTATCACTGTGATATACTGTTTGATGGCTCTATCGCTTAGTATGATGCAGAAGTATACTGATATAAACCCTGATGCAGCTTACTCTGTTGCCTTCGAGCGTGTTGGAATGAAATGGGCTAAGTATTTGGTGGCTCTTGGTGCTTTGAAGGGGATGACTACTGTTCTTCTGGTCGGGGCACTCGGGCAAGCTCGGTATACAACTCACATTGCTCGAGCCCATATGATTCCTCCTTGGTTTGCTCTTGTCCATCCAAAGACTGGAACTCCCATTAATGCTACACTCTTGATTACCATAGCAGGTGGCTGTATTGCTTTCTTCTCTAGCTTGGATATTCTGGCGAGCTTGTTGTCGGTCAGCACGCTCTTCGTCTTCATGATGATGGCCGTGGCACTTCTGGTAAGGAGATACTATGCAAGAGGGGTCACACCACGGATAGACCAGTTGAAGCTTTTCATTCTCCTGATAACCATCATTGGTTCCTCCATGGCTACATCTGCATACTGGGGGCTTTATCCAAATGGTTGGATAGGATACGTCGTGACCGTCCCGATTTGGTTTCTGGGGACTTTGGGGATTTCATTGCTTCTGCCAATGCAAAGAAAGCCAAAAGTTTGGGGCGTGCCATTGGTGCCATGGCTGCCGAGTTTGTCGATTGCGACGAACATCTTTCTCATGGGATCTTTGGGTCCTGAGGCTTTTGAAAGATTTGGAATCTGCACATTGGTGATGCTTATCTATTATTTTTTTTTTGGTCTTCATGCAACGTATGATATGGCTCATCAACAAGACAAATTAATTACCCAAAAGCAGGTTAATGAGGAAACCACGCCCAGTGCAGGTCCTTAG

mRNA sequence

ATGGGGGAAGAAGTTGTTCAGAAGCAGAGAAGCTATTGGTGGAGATGGAACAAACAAGATTTCTTACCAGAAGAATCTTTCCAGAGTTGGAGCAATTACGGAACTGCTCTGTCACAGACATGGTTTCGGTTCCTGGACCGTCTTCAAAGCAGATCACTCGATGAAAATGAAATTGGAGAGCTAAGAAAACGAAGTGAGAATGAAATGAAACGTTGCCTTACTTGGTGGGATCTCACTTGGTTTGGATTTGGAGCTGTAATTGGTGCAGGAATCTTCGTCCTCACAGGCCAAGAAGCCAACAAACATGCTGGACCAGCCATTGTTCTGTCTTATGTAGCTTCAGGCATTTCTGCAATGCTCTCTGTTTTTTGCTATACAGAGTTCGCTATTGAAATCCCAGTTGCAGGGGGATCTTTTGCATACTTAAGAATTGAATTGGGGGACTTTGCAGCTTTCATTACTGCAGGCAATATTCTCCTCGAGAGCATTGTCGGTACTGCCGCCGTCGCTCGAGCTTGGACTTCTTACTTTACCTCGCTTTTGGACCGACCCGACAACTCGTTGCGTATCCACACGAATCTCAAAGATGGATTCAATCTTCTCGACCCAATTGCCGTTGCGGTTTTAGCGATTGCTGCGACAATAGCGATGACCAGCACGAGAAAAACTTCATACCTGAATTGGATTGCTTCAGCTGTTAATACAGTTGTGATTCTGTTTGTTATAATTGCAGGTTTTGTACATGCTGATAAATCAAATTTGACTCCTTTTATGCCTTTTGGAGTTAAAGGTGTCTTCCAAGCTGCTGCAATTGTTTACTTTGCTTATGGAGGTTTTGATAACATTGCTACAATGGCTGAGGAAACTAAAAATCCATCAAAAGATATACCTTTGGGATTGTTGGGATCAATGTCGATTATCACTGTGATATACTGTTTGATGGCTCTATCGCTTAGTATGATGCAGAAGTATACTGATATAAACCCTGATGCAGCTTACTCTGTTGCCTTCGAGCGTGTTGGAATGAAATGGGCTAAGTATTTGGTGGCTCTTGGTGCTTTGAAGGGGATGACTACTGTTCTTCTGGTCGGGGCACTCGGGCAAGCTCGGTATACAACTCACATTGCTCGAGCCCATATGATTCCTCCTTGGTTTGCTCTTGTCCATCCAAAGACTGGAACTCCCATTAATGCTACACTCTTGATTACCATAGCAGGTGGCTGTATTGCTTTCTTCTCTAGCTTGGATATTCTGGCGAGCTTGTTGTCGGTCAGCACGCTCTTCGTCTTCATGATGATGGCCGTGGCACTTCTGGTAAGGAGATACTATGCAAGAGGGGTCACACCACGGATAGACCAGTTGAAGCTTTTCATTCTCCTGATAACCATCATTGGTTCCTCCATGGCTACATCTGCATACTGGGGGCTTTATCCAAATGGTTGGATAGGATACGTCGTGACCGTCCCGATTTGGTTTCTGGGGACTTTGGGGATTTCATTGCTTCTGCCAATGCAAAGAAAGCCAAAAGTTTGGGGCGTGCCATTGGTGCCATGGCTGCCGAGTTTGTCGATTGCGACGAACATCTTTCTCATGGGATCTTTGGGTCCTGAGGCTTTTGAAAGATTTGGAATCTGCACATTGGTGATGCTTATCTATTATTTTTTTTTTGGTCTTCATGCAACGTATGATATGGCTCATCAACAAGACAAATTAATTACCCAAAAGCAGGTTAATGAGGAAACCACGCCCAGTGCAGGTCCTTAG

Coding sequence (CDS)

ATGGGGGAAGAAGTTGTTCAGAAGCAGAGAAGCTATTGGTGGAGATGGAACAAACAAGATTTCTTACCAGAAGAATCTTTCCAGAGTTGGAGCAATTACGGAACTGCTCTGTCACAGACATGGTTTCGGTTCCTGGACCGTCTTCAAAGCAGATCACTCGATGAAAATGAAATTGGAGAGCTAAGAAAACGAAGTGAGAATGAAATGAAACGTTGCCTTACTTGGTGGGATCTCACTTGGTTTGGATTTGGAGCTGTAATTGGTGCAGGAATCTTCGTCCTCACAGGCCAAGAAGCCAACAAACATGCTGGACCAGCCATTGTTCTGTCTTATGTAGCTTCAGGCATTTCTGCAATGCTCTCTGTTTTTTGCTATACAGAGTTCGCTATTGAAATCCCAGTTGCAGGGGGATCTTTTGCATACTTAAGAATTGAATTGGGGGACTTTGCAGCTTTCATTACTGCAGGCAATATTCTCCTCGAGAGCATTGTCGGTACTGCCGCCGTCGCTCGAGCTTGGACTTCTTACTTTACCTCGCTTTTGGACCGACCCGACAACTCGTTGCGTATCCACACGAATCTCAAAGATGGATTCAATCTTCTCGACCCAATTGCCGTTGCGGTTTTAGCGATTGCTGCGACAATAGCGATGACCAGCACGAGAAAAACTTCATACCTGAATTGGATTGCTTCAGCTGTTAATACAGTTGTGATTCTGTTTGTTATAATTGCAGGTTTTGTACATGCTGATAAATCAAATTTGACTCCTTTTATGCCTTTTGGAGTTAAAGGTGTCTTCCAAGCTGCTGCAATTGTTTACTTTGCTTATGGAGGTTTTGATAACATTGCTACAATGGCTGAGGAAACTAAAAATCCATCAAAAGATATACCTTTGGGATTGTTGGGATCAATGTCGATTATCACTGTGATATACTGTTTGATGGCTCTATCGCTTAGTATGATGCAGAAGTATACTGATATAAACCCTGATGCAGCTTACTCTGTTGCCTTCGAGCGTGTTGGAATGAAATGGGCTAAGTATTTGGTGGCTCTTGGTGCTTTGAAGGGGATGACTACTGTTCTTCTGGTCGGGGCACTCGGGCAAGCTCGGTATACAACTCACATTGCTCGAGCCCATATGATTCCTCCTTGGTTTGCTCTTGTCCATCCAAAGACTGGAACTCCCATTAATGCTACACTCTTGATTACCATAGCAGGTGGCTGTATTGCTTTCTTCTCTAGCTTGGATATTCTGGCGAGCTTGTTGTCGGTCAGCACGCTCTTCGTCTTCATGATGATGGCCGTGGCACTTCTGGTAAGGAGATACTATGCAAGAGGGGTCACACCACGGATAGACCAGTTGAAGCTTTTCATTCTCCTGATAACCATCATTGGTTCCTCCATGGCTACATCTGCATACTGGGGGCTTTATCCAAATGGTTGGATAGGATACGTCGTGACCGTCCCGATTTGGTTTCTGGGGACTTTGGGGATTTCATTGCTTCTGCCAATGCAAAGAAAGCCAAAAGTTTGGGGCGTGCCATTGGTGCCATGGCTGCCGAGTTTGTCGATTGCGACGAACATCTTTCTCATGGGATCTTTGGGTCCTGAGGCTTTTGAAAGATTTGGAATCTGCACATTGGTGATGCTTATCTATTATTTTTTTTTTGGTCTTCATGCAACGTATGATATGGCTCATCAACAAGACAAATTAATTACCCAAAAGCAGGTTAATGAGGAAACCACGCCCAGTGCAGGTCCTTAG

Protein sequence

MGEEVVQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP
Homology
BLAST of Lsi02G005370 vs. ExPASy Swiss-Prot
Match: O64759 (Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 1.3e-249
Identity = 440/562 (78.29%), Postives = 489/562 (87.01%), Query Frame = 0

Query: 8   KQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSEN 67
           ++R Y WRW+K+DF PEESFQS+ +Y  ALSQT  RF +RL SRS DENE  EL+K+SE+
Sbjct: 4   EERGY-WRWSKRDFFPEESFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSEH 63

Query: 68  EMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTE 127
           EMKRCLTWWDL WFGFG+VIGAGIFVLTGQEA++ AGPAIVLSYV SG+SAMLSVFCYTE
Sbjct: 64  EMKRCLTWWDLVWFGFGSVIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTE 123

Query: 128 FAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNS 187
           FA+EIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYF +LL+R  N+
Sbjct: 124 FAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPNA 183

Query: 188 LRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFV 247
           LRI T+L  GFNLLDPIAV V+A +ATIA  STRKTS LNWIASA+NT+VI FVIIAGF+
Sbjct: 184 LRIKTDLSSGFNLLDPIAVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFI 243

Query: 248 HADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSII 307
           HAD SNLTPF+PFG +GVF+AAA+VYFAYGGFD+IATMAEETKNPS+DIP+GLLGSMSII
Sbjct: 244 HADTSNLTPFLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSII 303

Query: 308 TVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLLVGALG 367
           TVIYCLMALSLSMMQKYTDI+P+AAYSVAF+ VGMKW KYLVALGALKGMTTVLLVGALG
Sbjct: 304 TVIYCLMALSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALG 363

Query: 368 QARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTL 427
           QARY THIAR HMIPP FALVHPKTGTPINA LL+ I    IAFFS LD+LASLLS+STL
Sbjct: 364 QARYVTHIARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSISTL 423

Query: 428 FVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG-WIGYVV 487
           F+F MM +ALLVRRYY R  TPR+  +KL   L+ ++ SSM TSAYWG+   G WIGY V
Sbjct: 424 FIFTMMPIALLVRRYYVRQDTPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGYTV 483

Query: 488 TVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICT 547
           TVP WFLGTLGI   +P QR PKVWGVPLVPWLP LSIATNIFLMGSLG  AF RFG+CT
Sbjct: 484 TVPFWFLGTLGIVFFVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGVCT 543

Query: 548 LVMLIYYFFFGLHATYDMAHQQ 569
           L ML+YYF  GLHAT+DMAHQQ
Sbjct: 544 LAMLLYYFLLGLHATFDMAHQQ 564

BLAST of Lsi02G005370 vs. ExPASy Swiss-Prot
Match: Q9SHH0 (Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=CAT8 PE=2 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 2.0e-202
Identity = 356/560 (63.57%), Postives = 436/560 (77.86%), Query Frame = 0

Query: 9   QRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENE 68
           QR  +WRW KQDF PE SFQS+S Y +ALS T  R  DRL SRS D  E+   R+ SEN 
Sbjct: 21  QRRSYWRWRKQDFFPEFSFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENP 80

Query: 69  MKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEF 128
           M+RCLTWWDL W  FG+V+G+G+FV+TGQEA   AGPA+VLSY  SG+SA+LSV CY EF
Sbjct: 81  MRRCLTWWDLLWLSFGSVVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEF 140

Query: 129 AIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSL 188
            +EIPVAGGSF+YLR+ELGDF AFI AGNILLE++VG A + R+W+SY  SL+    +  
Sbjct: 141 GVEIPVAGGSFSYLRVELGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYF 200

Query: 189 RIHT-NLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFV 248
           RI   +   GF+LLDP+AVAVL +A  IAMT T++TS+LN I S V   +I+F+++ GF 
Sbjct: 201 RIKVDSFAKGFDLLDPVAVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFT 260

Query: 249 HADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSII 308
           H+  SNL PF P+G KGV Q+AA+VY++Y GFD +A MAEET+ PS+DIP+GL+GSMS+I
Sbjct: 261 HSKTSNLVPFFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMI 320

Query: 309 TVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLLVGALG 368
           TV+YCLMAL+L+MM KYT+I+ +AAYSVAF ++GMKWAKYLV + ALKGMTT LLVG+LG
Sbjct: 321 TVVYCLMALALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLG 380

Query: 369 QARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTL 428
           QARYTT IAR+HMIPPWFALVHPKTGTPI ATLL+TI    I+FF+SL++L+S+ S +TL
Sbjct: 381 QARYTTQIARSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATL 440

Query: 429 FVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWIGYVVT 488
           F+FM++AVALLVRRYY + VTP    LK    L  II SS+  SA W     GWI Y VT
Sbjct: 441 FIFMLVAVALLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVT 500

Query: 489 VPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICTL 548
             IWF+GTLG++ LLP  R PKVWGVPLVPWLPS SIA N+FL+GSLG  AF RF ICT+
Sbjct: 501 GVIWFIGTLGLA-LLPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTM 560

Query: 549 VMLIYYFFFGLHATYDMAHQ 568
           VML+YY F GLHATYD+AHQ
Sbjct: 561 VMLLYYLFVGLHATYDVAHQ 579

BLAST of Lsi02G005370 vs. ExPASy Swiss-Prot
Match: Q84MA5 (Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 1.1e-197
Identity = 351/549 (63.93%), Postives = 426/549 (77.60%), Query Frame = 0

Query: 18  KQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENEMKRCLTWWD 77
           K DFLPEESFQS  NY  AL +T  RF+DR+ +RSLD +EI E++ RS +EMK+ LTWWD
Sbjct: 19  KDDFLPEESFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWD 78

Query: 78  LTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEFAIEIPVAGG 137
           L WFG GAVIG+GIFVLTG EA  H+GPA+VLSYV SG+SAMLSVFCYTEFA+EIPVAGG
Sbjct: 79  LMWFGIGAVIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGG 138

Query: 138 SFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSLRIHTN-LKD 197
           SFAYLR+ELGDF AFI AGNI+LE +VG AAVAR+WTSYF +LL+      RI  + L +
Sbjct: 139 SFAYLRVELGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATLLNHKPEDFRIIVHKLGE 198

Query: 198 GFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFVHADKSNLTP 257
            ++ LDPIAV V AI   +A+  T+ +S  N+IAS ++ VVILFVIIAGF  AD  N + 
Sbjct: 199 DYSHLDPIAVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNYSD 258

Query: 258 FMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSIITVIYCLMAL 317
           F P+GV+GVF++AA+++FAY GFD ++TMAEETKNP +DIP+GL+GSM + TV YCLMA+
Sbjct: 259 FTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMAV 318

Query: 318 SLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLLVGALGQARYTTHIA 377
           +L +MQ Y  I+PDA +SVAF  VG  WAKY+VA GALKGMTTVLLVGA+GQARY THIA
Sbjct: 319 TLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHIA 378

Query: 378 RAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTLFVFMMMAVA 437
           RAHM+PPW A V+ KTGTPINAT+++  A   IAFF+ L ILA LLSVSTLF+FM +AVA
Sbjct: 379 RAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSVSTLFIFMFVAVA 438

Query: 438 LLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWIGYVVTVPIWFLGTL 497
           LLVRRYY  G T   D+ K  + L  I+ SS AT+ YW L   GWIGY +TVPIWFL T+
Sbjct: 439 LLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEEGWIGYCITVPIWFLSTV 498

Query: 498 GISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICTLVMLIYYFFF 557
            +  L+P  R PK+WGVPLVPWLPS SIA NIFL+GS+  ++F RF I T ++LIYY  F
Sbjct: 499 AMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAIWTGILLIYYVLF 558

Query: 558 GLHATYDMA 566
           GLHATYD A
Sbjct: 559 GLHATYDTA 567

BLAST of Lsi02G005370 vs. ExPASy Swiss-Prot
Match: Q9SQZ0 (Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT7 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.1e-115
Identity = 240/563 (42.63%), Postives = 350/563 (62.17%), Query Frame = 0

Query: 24  EESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENEMKRCLTWWDLTWFGF 83
           + SF S   Y  +LS T  RF  R  S S   +E+  +R  S  +M+R L W+DL   G 
Sbjct: 11  DTSFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGI 70

Query: 84  GAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEFAIEIPVAGGSFAYLR 143
           G +IGAG+FV TG+ +  +AGP+IV+SY  +G+ A+LS FCYTEFA+ +PVAGG+F+Y+R
Sbjct: 71  GGMIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIR 130

Query: 144 IELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSLR-IHTNLKDGFNLLD 203
           I  G+F AFIT  N++++ ++  AAV+R +T+Y  S      +  R I + L +GFN +D
Sbjct: 131 ITFGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEID 190

Query: 204 PIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFVHADKSNLT------- 263
           PIAV V+     +   STR++S +N + +A++   I+FVI+ GF   D  NLT       
Sbjct: 191 PIAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPEN 250

Query: 264 --PFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSIITVIYCL 323
              F PFGV GVF  AA+VY +Y G+D ++TMAEE K+P KDIP+G+ GS++I+ V+YCL
Sbjct: 251 PSGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCL 310

Query: 324 MALSLSMMQKYTDINPDAAYSVAFER-VGMKWAKYLVALGALKGMTTVLLVGALGQARYT 383
           MA+S+SM+  Y  I+ +A YS AF +  G +W   +V +GA  G+ T L+V  LGQARY 
Sbjct: 311 MAISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYM 370

Query: 384 THIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTLFVFMM 443
             I R+ ++P WFA VHPKT TP+NA+  + I    +A F+ L++L +L+S+ TLFVF M
Sbjct: 371 CVIGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYM 430

Query: 444 MAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWIGYVVTVPIWF 503
           +A A++ RRY   G T     L  F+ L +I  +S+  +  W L P+G        P WF
Sbjct: 431 VANAVIFRRYVTVGYTEPWPTLS-FLCLFSI--TSILFTLVWQLAPSG-------PPKWF 490

Query: 504 LGTLGIS------------LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFE 563
           +  LG S             ++P  R P+ WGVPL+PW P +SI  NIFL+GSL   ++ 
Sbjct: 491 I--LGASTVTAIAIVQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYI 550

BLAST of Lsi02G005370 vs. ExPASy Swiss-Prot
Match: Q9LZ20 (Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT6 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 7.1e-115
Identity = 234/554 (42.24%), Postives = 345/554 (62.27%), Query Frame = 0

Query: 26  SFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENEMKRCLTWWDLTWFGFGA 85
           SF S   Y  +LS T  R   R  S S   +E+  +R  S  +M+R L W+DL   G G 
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 86  VIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEFAIEIPVAGGSFAYLRIE 145
           ++GAG+FV TG+ +   AGP+IV+SY  +G+ A+LS FCYTEFA+ +PVAGG+F+Y+RI 
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 146 LGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSLR-IHTNLKDGFNLLDPI 205
            G+F AF T  N++++ ++  AAV+R++T+Y  +      +  R + + L  GFN +DP+
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDPV 193

Query: 206 AVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFVHADKSNLTP-------- 265
           AV V+ +   I   STR++S +N I +A +   I FVI+ GF+  D  NL+         
Sbjct: 194 AVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPS 253

Query: 266 -FMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSIITVIYCLMA 325
            F PFG  GVF  AA+VY +Y G+D ++TMAEE +NP KDIP+G+ GS++I+TV+YCLMA
Sbjct: 254 GFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMA 313

Query: 326 LSLSMMQKYTDINPDAAYSVAFE-RVGMKWAKYLVALGALKGMTTVLLVGALGQARYTTH 385
           +S+SM+  Y  I+P+A +S AF    G +W   +V +GA  G+ T LLV  LGQARY   
Sbjct: 314 VSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCV 373

Query: 386 IARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTLFVFMMMA 445
           I R+ ++P WFA +HPKT TP+NA+  + I    +A F+ L++L +L+S+ TLFVF M+A
Sbjct: 374 IGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVA 433

Query: 446 VALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG-----WIGYVVTVP 505
            AL+ RRY   G T     L  F+ L +I  +S+  +  W L P G      +G    V 
Sbjct: 434 NALIFRRYVPVGPTKPWPTL-CFLTLFSI--TSLVFTLIWKLVPEGKPKAFMLGASAVVA 493

Query: 506 IWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICTLVM 564
           I  +  L    ++P  RKP++WGVP +PW P +SI  NIFL+GSL   ++ RFG  + ++
Sbjct: 494 IAIV--LSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLI 553

BLAST of Lsi02G005370 vs. ExPASy TrEMBL
Match: A0A0A0LWU6 (AA_permease_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G056920 PE=4 SV=1)

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 558/588 (94.90%), Postives = 572/588 (97.28%), Query Frame = 0

Query: 1   MGEEV-VQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIG 60
           MGEEV V +QRSYWWRW+KQDFLPEESFQSWSNY TALSQTWFRF+DRLQSRS DENEIG
Sbjct: 1   MGEEVIVHQQRSYWWRWSKQDFLPEESFQSWSNYRTALSQTWFRFIDRLQSRSFDENEIG 60

Query: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAM 120
           ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEAN+HAGPAIVLSYVASGISAM
Sbjct: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANEHAGPAIVLSYVASGISAM 120

Query: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTS 180
           LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVAR+WTSYFTS
Sbjct: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARSWTSYFTS 180

Query: 181 LLDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVIL 240
           LLDRPD SL IHTNLKDG+NLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASA+NTVVIL
Sbjct: 181 LLDRPDKSLLIHTNLKDGYNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAINTVVIL 240

Query: 241 FVIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300
           FVIIAGF+HADKSNLTPF PFGVKG+FQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG
Sbjct: 241 FVIIAGFIHADKSNLTPFTPFGVKGIFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300

Query: 301 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360
           LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT
Sbjct: 301 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360

Query: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILA 420
           VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLI I  GCIAFFSSLD+LA
Sbjct: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLIAITSGCIAFFSSLDVLA 420

Query: 421 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPN 480
           SLLSVSTLFVFMMMAVALLVRRYYARGVTPR+DQLKLFILLI IIGSSMATSAYWGLYPN
Sbjct: 421 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRLDQLKLFILLILIIGSSMATSAYWGLYPN 480

Query: 481 GWIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF 540
           GWIGYVVTVP+WFLGTLGI+LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLG EAF
Sbjct: 481 GWIGYVVTVPVWFLGTLGIALLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGREAF 540

Query: 541 ERFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           ERFGICTLVMLIYY FFGLHATYDMAHQQDKL+TQKQV EET PSA P
Sbjct: 541 ERFGICTLVMLIYYVFFGLHATYDMAHQQDKLVTQKQVKEETPPSAVP 588

BLAST of Lsi02G005370 vs. ExPASy TrEMBL
Match: A0A6J1EAW6 (cationic amino acid transporter 5 OS=Cucurbita moschata OX=3662 GN=LOC111431495 PE=4 SV=1)

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 553/587 (94.21%), Postives = 570/587 (97.10%), Query Frame = 0

Query: 1   MGEEVVQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGE 60
           MG+++VQ+QRSYWWRW+KQDFLPEESFQS SNY TALSQTWFRF+DRLQSRS DENEIGE
Sbjct: 9   MGDDIVQQQRSYWWRWSKQDFLPEESFQSLSNYRTALSQTWFRFMDRLQSRSFDENEIGE 68

Query: 61  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAML 120
           LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASG+SAML
Sbjct: 69  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGVSAML 128

Query: 121 SVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSL 180
           SVFCYTEFA+EIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSY TSL
Sbjct: 129 SVFCYTEFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYLTSL 188

Query: 181 LDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILF 240
           LDRPDNSLRIHTNLKDGFNLLDPIAV VL IAATIAM STRKTSYLNWIASAVNTVVILF
Sbjct: 189 LDRPDNSLRIHTNLKDGFNLLDPIAVGVLTIAATIAMISTRKTSYLNWIASAVNTVVILF 248

Query: 241 VIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 300
           VIIAGFVHADKSNLTPF+PFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL
Sbjct: 249 VIIAGFVHADKSNLTPFVPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 308

Query: 301 LGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTV 360
           LGSMS+ITVIYCLMALSLSMMQKYTDINPDAAYSVAFE VGMKWAKYLVALGALKGMTTV
Sbjct: 309 LGSMSVITVIYCLMALSLSMMQKYTDINPDAAYSVAFESVGMKWAKYLVALGALKGMTTV 368

Query: 361 LLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILAS 420
           LLVGALGQ RY THIARAHMIPPWFALVHPKTGTPINATLLIT+A G +AFFSSLD+LAS
Sbjct: 369 LLVGALGQGRYITHIARAHMIPPWFALVHPKTGTPINATLLITVASGSVAFFSSLDVLAS 428

Query: 421 LLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG 480
           LLSVSTLFVFMMMAVALLVRRYYARGVTPR  QLKLFILLITII SSMATSAYWGLYPNG
Sbjct: 429 LLSVSTLFVFMMMAVALLVRRYYARGVTPRSHQLKLFILLITIIASSMATSAYWGLYPNG 488

Query: 481 WIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFE 540
           W+GYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATN+FLMGSLGPEAFE
Sbjct: 489 WVGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNVFLMGSLGPEAFE 548

Query: 541 RFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           RFGICTLVMLIYY FFGLHATYDMAHQ DKL+TQKQ+NEETTPSAGP
Sbjct: 549 RFGICTLVMLIYYVFFGLHATYDMAHQDDKLMTQKQINEETTPSAGP 595

BLAST of Lsi02G005370 vs. ExPASy TrEMBL
Match: A0A5D3BJV9 (Cationic amino acid transporter 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005070 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 556/588 (94.56%), Postives = 571/588 (97.11%), Query Frame = 0

Query: 1   MGEEV-VQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIG 60
           MGEEV V +QRSYWWRW+KQDFLPEESFQSWSNY TALSQTWFRF+DRLQSRS DENEIG
Sbjct: 1   MGEEVIVHEQRSYWWRWSKQDFLPEESFQSWSNYRTALSQTWFRFIDRLQSRSFDENEIG 60

Query: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAM 120
           ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEAN+HAGPAIVLSYVASGISAM
Sbjct: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANEHAGPAIVLSYVASGISAM 120

Query: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTS 180
           LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVAR+WTSYFTS
Sbjct: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARSWTSYFTS 180

Query: 181 LLDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVIL 240
           LLDRPD SL IHTNLKDG+NLLDPIAV VLAIAATIAMTSTRKTSYLNWIASA+NTVVIL
Sbjct: 181 LLDRPDKSLLIHTNLKDGYNLLDPIAVGVLAIAATIAMTSTRKTSYLNWIASAINTVVIL 240

Query: 241 FVIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300
           FVIIAGFVHADKSNLTPF PFGVKG+FQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG
Sbjct: 241 FVIIAGFVHADKSNLTPFTPFGVKGIFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300

Query: 301 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360
           LLGSMSIITVIYCLMALSLSMMQKYTDI+PDAAYSVAFERVGMKWAKYLVALGALKGMTT
Sbjct: 301 LLGSMSIITVIYCLMALSLSMMQKYTDISPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360

Query: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILA 420
           VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLI I  GCIAFFSSLD+LA
Sbjct: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLIAITSGCIAFFSSLDVLA 420

Query: 421 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPN 480
           SLLSVSTLFVF MMAVALLVRRYYARGVTPR+DQLKLFILLI IIGSSMATSAYWGLYPN
Sbjct: 421 SLLSVSTLFVFTMMAVALLVRRYYARGVTPRLDQLKLFILLIIIIGSSMATSAYWGLYPN 480

Query: 481 GWIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF 540
           GWIGYVVTVP+WFLGTLGI+LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF
Sbjct: 481 GWIGYVVTVPVWFLGTLGIALLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF 540

Query: 541 ERFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           ERFGICTLVML+YY FFGLHATYDMAHQQDKLIT K+V EET PSAGP
Sbjct: 541 ERFGICTLVMLVYYVFFGLHATYDMAHQQDKLITPKKVQEETMPSAGP 588

BLAST of Lsi02G005370 vs. ExPASy TrEMBL
Match: A0A1S3B1F8 (cationic amino acid transporter 5 OS=Cucumis melo OX=3656 GN=LOC103484797 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 556/588 (94.56%), Postives = 571/588 (97.11%), Query Frame = 0

Query: 1   MGEEV-VQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIG 60
           MGEEV V +QRSYWWRW+KQDFLPEESFQSWSNY TALSQTWFRF+DRLQSRS DENEIG
Sbjct: 11  MGEEVIVHEQRSYWWRWSKQDFLPEESFQSWSNYRTALSQTWFRFIDRLQSRSFDENEIG 70

Query: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAM 120
           ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEAN+HAGPAIVLSYVASGISAM
Sbjct: 71  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANEHAGPAIVLSYVASGISAM 130

Query: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTS 180
           LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVAR+WTSYFTS
Sbjct: 131 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARSWTSYFTS 190

Query: 181 LLDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVIL 240
           LLDRPD SL IHTNLKDG+NLLDPIAV VLAIAATIAMTSTRKTSYLNWIASA+NTVVIL
Sbjct: 191 LLDRPDKSLLIHTNLKDGYNLLDPIAVGVLAIAATIAMTSTRKTSYLNWIASAINTVVIL 250

Query: 241 FVIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300
           FVIIAGFVHADKSNLTPF PFGVKG+FQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG
Sbjct: 251 FVIIAGFVHADKSNLTPFTPFGVKGIFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 310

Query: 301 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360
           LLGSMSIITVIYCLMALSLSMMQKYTDI+PDAAYSVAFERVGMKWAKYLVALGALKGMTT
Sbjct: 311 LLGSMSIITVIYCLMALSLSMMQKYTDISPDAAYSVAFERVGMKWAKYLVALGALKGMTT 370

Query: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILA 420
           VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLI I  GCIAFFSSLD+LA
Sbjct: 371 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLIAITSGCIAFFSSLDVLA 430

Query: 421 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPN 480
           SLLSVSTLFVF MMAVALLVRRYYARGVTPR+DQLKLFILLI IIGSSMATSAYWGLYPN
Sbjct: 431 SLLSVSTLFVFTMMAVALLVRRYYARGVTPRLDQLKLFILLIIIIGSSMATSAYWGLYPN 490

Query: 481 GWIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF 540
           GWIGYVVTVP+WFLGTLGI+LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF
Sbjct: 491 GWIGYVVTVPVWFLGTLGIALLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF 550

Query: 541 ERFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           ERFGICTLVML+YY FFGLHATYDMAHQQDKLIT K+V EET PSAGP
Sbjct: 551 ERFGICTLVMLVYYVFFGLHATYDMAHQQDKLITPKKVQEETMPSAGP 598

BLAST of Lsi02G005370 vs. ExPASy TrEMBL
Match: A0A6J1KGQ1 (cationic amino acid transporter 5 OS=Cucurbita maxima OX=3661 GN=LOC111495108 PE=4 SV=1)

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 552/587 (94.04%), Postives = 568/587 (96.76%), Query Frame = 0

Query: 1   MGEEVVQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGE 60
           MG+ +VQ+QRSYWWRW+KQDFLPEESFQS SNY TALSQTWFRF+DRLQSRS DENEIGE
Sbjct: 1   MGDHIVQQQRSYWWRWSKQDFLPEESFQSLSNYRTALSQTWFRFMDRLQSRSFDENEIGE 60

Query: 61  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAML 120
           LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASG+SAML
Sbjct: 61  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGVSAML 120

Query: 121 SVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSL 180
           SVFCYTEFA+EIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSY TSL
Sbjct: 121 SVFCYTEFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYLTSL 180

Query: 181 LDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILF 240
           LDRPDNSLRIHTNLKDGFNLLDPIAV VL IAATIAM STRKTSYLNWIASAVNTVVILF
Sbjct: 181 LDRPDNSLRIHTNLKDGFNLLDPIAVGVLTIAATIAMISTRKTSYLNWIASAVNTVVILF 240

Query: 241 VIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 300
           VIIAGFVHADKSNLTPF+PFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL
Sbjct: 241 VIIAGFVHADKSNLTPFVPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 300

Query: 301 LGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTV 360
           LGSMS+ITVIYCLMALSLSMMQKYTDINPDAAYSVAFE VGMKWAKYLVALGALKGMTTV
Sbjct: 301 LGSMSVITVIYCLMALSLSMMQKYTDINPDAAYSVAFESVGMKWAKYLVALGALKGMTTV 360

Query: 361 LLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILAS 420
           LLVGALGQ RY THIARAHMIPPWFALVHPKTGTPINATLLIT+A G +AFFSSLD+LAS
Sbjct: 361 LLVGALGQGRYITHIARAHMIPPWFALVHPKTGTPINATLLITVASGSVAFFSSLDVLAS 420

Query: 421 LLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG 480
           LLSVSTLFVFMMMAVALLVRRYYARGVTPR  QLKLFILLITII SSMATSAYWGLYPNG
Sbjct: 421 LLSVSTLFVFMMMAVALLVRRYYARGVTPRSHQLKLFILLITIIASSMATSAYWGLYPNG 480

Query: 481 WIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFE 540
           W+GYVVTVPIWFLGTLGISLLLPMQRKP VWGVPLVPWLPSLSIATN+FLMGSLGPEAFE
Sbjct: 481 WVGYVVTVPIWFLGTLGISLLLPMQRKPNVWGVPLVPWLPSLSIATNVFLMGSLGPEAFE 540

Query: 541 RFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           RFGICTLVMLIYY FFGLHATYDMAHQ DKL+TQKQ+NEETTPSAGP
Sbjct: 541 RFGICTLVMLIYYVFFGLHATYDMAHQDDKLMTQKQINEETTPSAGP 587

BLAST of Lsi02G005370 vs. NCBI nr
Match: XP_038876967.1 (cationic amino acid transporter 5 [Benincasa hispida])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 562/585 (96.07%), Postives = 575/585 (98.29%), Query Frame = 0

Query: 3   EEVVQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELR 62
           EE+VQ+ RSYWWRW+KQDFLPEESFQSWSNY TALSQTWFRF+DRLQSRS DENEIGELR
Sbjct: 50  EEIVQQHRSYWWRWSKQDFLPEESFQSWSNYRTALSQTWFRFMDRLQSRSFDENEIGELR 109

Query: 63  KRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSV 122
           KRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEAN+HAGPAIVLSYVASGISAMLSV
Sbjct: 110 KRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANQHAGPAIVLSYVASGISAMLSV 169

Query: 123 FCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLD 182
           FCYTEFAIEIPVAGGSFAYLRIELGDF AFITAGNILLESIVGTAAVAR+WTSYFTSLL+
Sbjct: 170 FCYTEFAIEIPVAGGSFAYLRIELGDFVAFITAGNILLESIVGTAAVARSWTSYFTSLLN 229

Query: 183 RPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVI 242
           RPD SL IHTNLKDG+NLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVN+VVILFVI
Sbjct: 230 RPDKSLLIHTNLKDGYNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNSVVILFVI 289

Query: 243 IAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLG 302
           IAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLG
Sbjct: 290 IAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLG 349

Query: 303 SMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLL 362
           SMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFE VGMKWAKYLVALGALKGMTTVLL
Sbjct: 350 SMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFESVGMKWAKYLVALGALKGMTTVLL 409

Query: 363 VGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLL 422
           VGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITI  GCIAFFSSLD+LASLL
Sbjct: 410 VGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITITSGCIAFFSSLDVLASLL 469

Query: 423 SVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWI 482
           SVSTLFVFMMMAVALLVRRYYARGVTPR+DQLKLFILLITIIGSSMATSAYWGLYPNGWI
Sbjct: 470 SVSTLFVFMMMAVALLVRRYYARGVTPRLDQLKLFILLITIIGSSMATSAYWGLYPNGWI 529

Query: 483 GYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERF 542
           GYVVTVPIWFLGTLGI+LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERF
Sbjct: 530 GYVVTVPIWFLGTLGIALLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERF 589

Query: 543 GICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           GICTLVMLIYY FFGLHATYDMAHQQ+KLITQKQVNEETTPSAGP
Sbjct: 590 GICTLVMLIYYVFFGLHATYDMAHQQNKLITQKQVNEETTPSAGP 634

BLAST of Lsi02G005370 vs. NCBI nr
Match: XP_004145133.2 (cationic amino acid transporter 5 [Cucumis sativus])

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 558/588 (94.90%), Postives = 572/588 (97.28%), Query Frame = 0

Query: 1   MGEEV-VQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIG 60
           MGEEV V +QRSYWWRW+KQDFLPEESFQSWSNY TALSQTWFRF+DRLQSRS DENEIG
Sbjct: 11  MGEEVIVHQQRSYWWRWSKQDFLPEESFQSWSNYRTALSQTWFRFIDRLQSRSFDENEIG 70

Query: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAM 120
           ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEAN+HAGPAIVLSYVASGISAM
Sbjct: 71  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANEHAGPAIVLSYVASGISAM 130

Query: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTS 180
           LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVAR+WTSYFTS
Sbjct: 131 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARSWTSYFTS 190

Query: 181 LLDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVIL 240
           LLDRPD SL IHTNLKDG+NLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASA+NTVVIL
Sbjct: 191 LLDRPDKSLLIHTNLKDGYNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAINTVVIL 250

Query: 241 FVIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300
           FVIIAGF+HADKSNLTPF PFGVKG+FQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG
Sbjct: 251 FVIIAGFIHADKSNLTPFTPFGVKGIFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 310

Query: 301 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360
           LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT
Sbjct: 311 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 370

Query: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILA 420
           VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLI I  GCIAFFSSLD+LA
Sbjct: 371 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLIAITSGCIAFFSSLDVLA 430

Query: 421 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPN 480
           SLLSVSTLFVFMMMAVALLVRRYYARGVTPR+DQLKLFILLI IIGSSMATSAYWGLYPN
Sbjct: 431 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRLDQLKLFILLILIIGSSMATSAYWGLYPN 490

Query: 481 GWIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF 540
           GWIGYVVTVP+WFLGTLGI+LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLG EAF
Sbjct: 491 GWIGYVVTVPVWFLGTLGIALLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGREAF 550

Query: 541 ERFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           ERFGICTLVMLIYY FFGLHATYDMAHQQDKL+TQKQV EET PSA P
Sbjct: 551 ERFGICTLVMLIYYVFFGLHATYDMAHQQDKLVTQKQVKEETPPSAVP 598

BLAST of Lsi02G005370 vs. NCBI nr
Match: KGN64456.1 (hypothetical protein Csa_013223 [Cucumis sativus])

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 558/588 (94.90%), Postives = 572/588 (97.28%), Query Frame = 0

Query: 1   MGEEV-VQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIG 60
           MGEEV V +QRSYWWRW+KQDFLPEESFQSWSNY TALSQTWFRF+DRLQSRS DENEIG
Sbjct: 1   MGEEVIVHQQRSYWWRWSKQDFLPEESFQSWSNYRTALSQTWFRFIDRLQSRSFDENEIG 60

Query: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAM 120
           ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEAN+HAGPAIVLSYVASGISAM
Sbjct: 61  ELRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANEHAGPAIVLSYVASGISAM 120

Query: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTS 180
           LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVAR+WTSYFTS
Sbjct: 121 LSVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARSWTSYFTS 180

Query: 181 LLDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVIL 240
           LLDRPD SL IHTNLKDG+NLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASA+NTVVIL
Sbjct: 181 LLDRPDKSLLIHTNLKDGYNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAINTVVIL 240

Query: 241 FVIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300
           FVIIAGF+HADKSNLTPF PFGVKG+FQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG
Sbjct: 241 FVIIAGFIHADKSNLTPFTPFGVKGIFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLG 300

Query: 301 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360
           LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT
Sbjct: 301 LLGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTT 360

Query: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILA 420
           VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLI I  GCIAFFSSLD+LA
Sbjct: 361 VLLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLIAITSGCIAFFSSLDVLA 420

Query: 421 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPN 480
           SLLSVSTLFVFMMMAVALLVRRYYARGVTPR+DQLKLFILLI IIGSSMATSAYWGLYPN
Sbjct: 421 SLLSVSTLFVFMMMAVALLVRRYYARGVTPRLDQLKLFILLILIIGSSMATSAYWGLYPN 480

Query: 481 GWIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAF 540
           GWIGYVVTVP+WFLGTLGI+LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLG EAF
Sbjct: 481 GWIGYVVTVPVWFLGTLGIALLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGREAF 540

Query: 541 ERFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           ERFGICTLVMLIYY FFGLHATYDMAHQQDKL+TQKQV EET PSA P
Sbjct: 541 ERFGICTLVMLIYYVFFGLHATYDMAHQQDKLVTQKQVKEETPPSAVP 588

BLAST of Lsi02G005370 vs. NCBI nr
Match: XP_022923923.1 (cationic amino acid transporter 5 [Cucurbita moschata])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 553/587 (94.21%), Postives = 570/587 (97.10%), Query Frame = 0

Query: 1   MGEEVVQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGE 60
           MG+++VQ+QRSYWWRW+KQDFLPEESFQS SNY TALSQTWFRF+DRLQSRS DENEIGE
Sbjct: 9   MGDDIVQQQRSYWWRWSKQDFLPEESFQSLSNYRTALSQTWFRFMDRLQSRSFDENEIGE 68

Query: 61  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAML 120
           LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASG+SAML
Sbjct: 69  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGVSAML 128

Query: 121 SVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSL 180
           SVFCYTEFA+EIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSY TSL
Sbjct: 129 SVFCYTEFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYLTSL 188

Query: 181 LDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILF 240
           LDRPDNSLRIHTNLKDGFNLLDPIAV VL IAATIAM STRKTSYLNWIASAVNTVVILF
Sbjct: 189 LDRPDNSLRIHTNLKDGFNLLDPIAVGVLTIAATIAMISTRKTSYLNWIASAVNTVVILF 248

Query: 241 VIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 300
           VIIAGFVHADKSNLTPF+PFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL
Sbjct: 249 VIIAGFVHADKSNLTPFVPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 308

Query: 301 LGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTV 360
           LGSMS+ITVIYCLMALSLSMMQKYTDINPDAAYSVAFE VGMKWAKYLVALGALKGMTTV
Sbjct: 309 LGSMSVITVIYCLMALSLSMMQKYTDINPDAAYSVAFESVGMKWAKYLVALGALKGMTTV 368

Query: 361 LLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILAS 420
           LLVGALGQ RY THIARAHMIPPWFALVHPKTGTPINATLLIT+A G +AFFSSLD+LAS
Sbjct: 369 LLVGALGQGRYITHIARAHMIPPWFALVHPKTGTPINATLLITVASGSVAFFSSLDVLAS 428

Query: 421 LLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG 480
           LLSVSTLFVFMMMAVALLVRRYYARGVTPR  QLKLFILLITII SSMATSAYWGLYPNG
Sbjct: 429 LLSVSTLFVFMMMAVALLVRRYYARGVTPRSHQLKLFILLITIIASSMATSAYWGLYPNG 488

Query: 481 WIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFE 540
           W+GYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATN+FLMGSLGPEAFE
Sbjct: 489 WVGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNVFLMGSLGPEAFE 548

Query: 541 RFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           RFGICTLVMLIYY FFGLHATYDMAHQ DKL+TQKQ+NEETTPSAGP
Sbjct: 549 RFGICTLVMLIYYVFFGLHATYDMAHQDDKLMTQKQINEETTPSAGP 595

BLAST of Lsi02G005370 vs. NCBI nr
Match: KAG6584181.1 (Cationic amino acid transporter 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019772.1 Cationic amino acid transporter 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 553/587 (94.21%), Postives = 570/587 (97.10%), Query Frame = 0

Query: 1   MGEEVVQKQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGE 60
           MG+++VQ+QRSYWWRW+KQDFLPEESFQS SNY TALSQTWFRF+DRLQSRS DENEIGE
Sbjct: 1   MGDDIVQQQRSYWWRWSKQDFLPEESFQSLSNYRTALSQTWFRFMDRLQSRSFDENEIGE 60

Query: 61  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAML 120
           LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASG+SAML
Sbjct: 61  LRKRSENEMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGVSAML 120

Query: 121 SVFCYTEFAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSL 180
           SVFCYTEFA+EIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSY TSL
Sbjct: 121 SVFCYTEFAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYLTSL 180

Query: 181 LDRPDNSLRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILF 240
           LDRPDNSLRIHTNLKDGFNLLDPIAV VL IAATIAM STRKTSYLNWIASAVNTVVILF
Sbjct: 181 LDRPDNSLRIHTNLKDGFNLLDPIAVGVLTIAATIAMISTRKTSYLNWIASAVNTVVILF 240

Query: 241 VIIAGFVHADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 300
           VIIAGFVHADKSNLTPF+PFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL
Sbjct: 241 VIIAGFVHADKSNLTPFVPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGL 300

Query: 301 LGSMSIITVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTV 360
           LGSMS+ITVIYCLMALSLSMMQKYTDINPDAAYSVAFE VGMKWAKYLVALGALKGMTTV
Sbjct: 301 LGSMSVITVIYCLMALSLSMMQKYTDINPDAAYSVAFESVGMKWAKYLVALGALKGMTTV 360

Query: 361 LLVGALGQARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILAS 420
           LLVGALGQ RY THIARAHMIPPWFALVHPKTGTPINATLLIT+A G +AFFSSLD+LAS
Sbjct: 361 LLVGALGQGRYITHIARAHMIPPWFALVHPKTGTPINATLLITVASGSVAFFSSLDVLAS 420

Query: 421 LLSVSTLFVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG 480
           LLSVSTLFVFMMMAVALLVRRYYARGVTPR  QLKLFILLITII SSMATSAYWGLYPNG
Sbjct: 421 LLSVSTLFVFMMMAVALLVRRYYARGVTPRSHQLKLFILLITIIASSMATSAYWGLYPNG 480

Query: 481 WIGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFE 540
           W+GYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATN+FLMGSLGPEAFE
Sbjct: 481 WVGYVVTVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNVFLMGSLGPEAFE 540

Query: 541 RFGICTLVMLIYYFFFGLHATYDMAHQQDKLITQKQVNEETTPSAGP 588
           RFGICTLVMLIYY FFGLHATYDMAHQ DKL+TQKQ+NEETTPSAGP
Sbjct: 541 RFGICTLVMLIYYVFFGLHATYDMAHQDDKLMTQKQINEETTPSAGP 587

BLAST of Lsi02G005370 vs. TAIR 10
Match: AT2G34960.1 (cationic amino acid transporter 5 )

HSP 1 Score: 863.6 bits (2230), Expect = 9.1e-251
Identity = 440/562 (78.29%), Postives = 489/562 (87.01%), Query Frame = 0

Query: 8   KQRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSEN 67
           ++R Y WRW+K+DF PEESFQS+ +Y  ALSQT  RF +RL SRS DENE  EL+K+SE+
Sbjct: 4   EERGY-WRWSKRDFFPEESFQSFGSYRAALSQTCSRFKNRLVSRSDDENERFELKKQSEH 63

Query: 68  EMKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTE 127
           EMKRCLTWWDL WFGFG+VIGAGIFVLTGQEA++ AGPAIVLSYV SG+SAMLSVFCYTE
Sbjct: 64  EMKRCLTWWDLVWFGFGSVIGAGIFVLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTE 123

Query: 128 FAIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNS 187
           FA+EIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYF +LL+R  N+
Sbjct: 124 FAVEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFATLLNRSPNA 183

Query: 188 LRIHTNLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFV 247
           LRI T+L  GFNLLDPIAV V+A +ATIA  STRKTS LNWIASA+NT+VI FVIIAGF+
Sbjct: 184 LRIKTDLSSGFNLLDPIAVVVIAASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFI 243

Query: 248 HADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSII 307
           HAD SNLTPF+PFG +GVF+AAA+VYFAYGGFD+IATMAEETKNPS+DIP+GLLGSMSII
Sbjct: 244 HADTSNLTPFLPFGPEGVFRAAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSII 303

Query: 308 TVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLLVGALG 367
           TVIYCLMALSLSMMQKYTDI+P+AAYSVAF+ VGMKW KYLVALGALKGMTTVLLVGALG
Sbjct: 304 TVIYCLMALSLSMMQKYTDIDPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALG 363

Query: 368 QARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTL 427
           QARY THIAR HMIPP FALVHPKTGTPINA LL+ I    IAFFS LD+LASLLS+STL
Sbjct: 364 QARYVTHIARTHMIPPIFALVHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSISTL 423

Query: 428 FVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG-WIGYVV 487
           F+F MM +ALLVRRYY R  TPR+  +KL   L+ ++ SSM TSAYWG+   G WIGY V
Sbjct: 424 FIFTMMPIALLVRRYYVRQDTPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGYTV 483

Query: 488 TVPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICT 547
           TVP WFLGTLGI   +P QR PKVWGVPLVPWLP LSIATNIFLMGSLG  AF RFG+CT
Sbjct: 484 TVPFWFLGTLGIVFFVPQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGVCT 543

Query: 548 LVMLIYYFFFGLHATYDMAHQQ 569
           L ML+YYF  GLHAT+DMAHQQ
Sbjct: 544 LAMLLYYFLLGLHATFDMAHQQ 564

BLAST of Lsi02G005370 vs. TAIR 10
Match: AT1G17120.1 (cationic amino acid transporter 8 )

HSP 1 Score: 706.8 bits (1823), Expect = 1.4e-203
Identity = 356/560 (63.57%), Postives = 436/560 (77.86%), Query Frame = 0

Query: 9   QRSYWWRWNKQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENE 68
           QR  +WRW KQDF PE SFQS+S Y +ALS T  R  DRL SRS D  E+   R+ SEN 
Sbjct: 21  QRRSYWRWRKQDFFPEFSFQSFSTYKSALSATCPRLADRLLSRSSDAYELDAARRESENP 80

Query: 69  MKRCLTWWDLTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEF 128
           M+RCLTWWDL W  FG+V+G+G+FV+TGQEA   AGPA+VLSY  SG+SA+LSV CY EF
Sbjct: 81  MRRCLTWWDLLWLSFGSVVGSGVFVITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEF 140

Query: 129 AIEIPVAGGSFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSL 188
            +EIPVAGGSF+YLR+ELGDF AFI AGNILLE++VG A + R+W+SY  SL+    +  
Sbjct: 141 GVEIPVAGGSFSYLRVELGDFIAFIAAGNILLEAMVGAAGLGRSWSSYLASLVKNDSDYF 200

Query: 189 RIHT-NLKDGFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFV 248
           RI   +   GF+LLDP+AVAVL +A  IAMT T++TS+LN I S V   +I+F+++ GF 
Sbjct: 201 RIKVDSFAKGFDLLDPVAVAVLLVANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFT 260

Query: 249 HADKSNLTPFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSII 308
           H+  SNL PF P+G KGV Q+AA+VY++Y GFD +A MAEET+ PS+DIP+GL+GSMS+I
Sbjct: 261 HSKTSNLVPFFPYGAKGVVQSAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMI 320

Query: 309 TVIYCLMALSLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLLVGALG 368
           TV+YCLMAL+L+MM KYT+I+ +AAYSVAF ++GMKWAKYLV + ALKGMTT LLVG+LG
Sbjct: 321 TVVYCLMALALTMMVKYTEIDANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLG 380

Query: 369 QARYTTHIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTL 428
           QARYTT IAR+HMIPPWFALVHPKTGTPI ATLL+TI    I+FF+SL++L+S+ S +TL
Sbjct: 381 QARYTTQIARSHMIPPWFALVHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATL 440

Query: 429 FVFMMMAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWIGYVVT 488
           F+FM++AVALLVRRYY + VTP    LK    L  II SS+  SA W     GWI Y VT
Sbjct: 441 FIFMLVAVALLVRRYYVKDVTPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVT 500

Query: 489 VPIWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICTL 548
             IWF+GTLG++ LLP  R PKVWGVPLVPWLPS SIA N+FL+GSLG  AF RF ICT+
Sbjct: 501 GVIWFIGTLGLA-LLPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTM 560

Query: 549 VMLIYYFFFGLHATYDMAHQ 568
           VML+YY F GLHATYD+AHQ
Sbjct: 561 VMLLYYLFVGLHATYDVAHQ 579

BLAST of Lsi02G005370 vs. TAIR 10
Match: AT4G21120.1 (amino acid transporter 1 )

HSP 1 Score: 691.0 bits (1782), Expect = 8.1e-199
Identity = 351/549 (63.93%), Postives = 426/549 (77.60%), Query Frame = 0

Query: 18  KQDFLPEESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENEMKRCLTWWD 77
           K DFLPEESFQS  NY  AL +T  RF+DR+ +RSLD +EI E++ RS +EMK+ LTWWD
Sbjct: 19  KDDFLPEESFQSMGNYLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWD 78

Query: 78  LTWFGFGAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEFAIEIPVAGG 137
           L WFG GAVIG+GIFVLTG EA  H+GPA+VLSYV SG+SAMLSVFCYTEFA+EIPVAGG
Sbjct: 79  LMWFGIGAVIGSGIFVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGG 138

Query: 138 SFAYLRIELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSLRIHTN-LKD 197
           SFAYLR+ELGDF AFI AGNI+LE +VG AAVAR+WTSYF +LL+      RI  + L +
Sbjct: 139 SFAYLRVELGDFMAFIAAGNIILEYVVGGAAVARSWTSYFATLLNHKPEDFRIIVHKLGE 198

Query: 198 GFNLLDPIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFVHADKSNLTP 257
            ++ LDPIAV V AI   +A+  T+ +S  N+IAS ++ VVILFVIIAGF  AD  N + 
Sbjct: 199 DYSHLDPIAVGVCAIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNYSD 258

Query: 258 FMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSIITVIYCLMAL 317
           F P+GV+GVF++AA+++FAY GFD ++TMAEETKNP +DIP+GL+GSM + TV YCLMA+
Sbjct: 259 FTPYGVRGVFKSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMAV 318

Query: 318 SLSMMQKYTDINPDAAYSVAFERVGMKWAKYLVALGALKGMTTVLLVGALGQARYTTHIA 377
           +L +MQ Y  I+PDA +SVAF  VG  WAKY+VA GALKGMTTVLLVGA+GQARY THIA
Sbjct: 319 TLCLMQPYQQIDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHIA 378

Query: 378 RAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTLFVFMMMAVA 437
           RAHM+PPW A V+ KTGTPINAT+++  A   IAFF+ L ILA LLSVSTLF+FM +AVA
Sbjct: 379 RAHMMPPWLAQVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSVSTLFIFMFVAVA 438

Query: 438 LLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWIGYVVTVPIWFLGTL 497
           LLVRRYY  G T   D+ K  + L  I+ SS AT+ YW L   GWIGY +TVPIWFL T+
Sbjct: 439 LLVRRYYVTGETSTRDRNKFLVFLGLILASSTATAVYWALEEEGWIGYCITVPIWFLSTV 498

Query: 498 GISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICTLVMLIYYFFF 557
            +  L+P  R PK+WGVPLVPWLPS SIA NIFL+GS+  ++F RF I T ++LIYY  F
Sbjct: 499 AMKFLVPQARAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAIWTGILLIYYVLF 558

Query: 558 GLHATYDMA 566
           GLHATYD A
Sbjct: 559 GLHATYDTA 567

BLAST of Lsi02G005370 vs. TAIR 10
Match: AT3G10600.1 (cationic amino acid transporter 7 )

HSP 1 Score: 418.7 bits (1075), Expect = 7.7e-117
Identity = 240/563 (42.63%), Postives = 350/563 (62.17%), Query Frame = 0

Query: 24  EESFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENEMKRCLTWWDLTWFGF 83
           + SF S   Y  +LS T  RF  R  S S   +E+  +R  S  +M+R L W+DL   G 
Sbjct: 11  DTSFSSLRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGI 70

Query: 84  GAVIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEFAIEIPVAGGSFAYLR 143
           G +IGAG+FV TG+ +  +AGP+IV+SY  +G+ A+LS FCYTEFA+ +PVAGG+F+Y+R
Sbjct: 71  GGMIGAGVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIR 130

Query: 144 IELGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSLR-IHTNLKDGFNLLD 203
           I  G+F AFIT  N++++ ++  AAV+R +T+Y  S      +  R I + L +GFN +D
Sbjct: 131 ITFGEFPAFITGANLIMDYVLSNAAVSRGFTAYLGSAFGISTSEWRFIVSGLPNGFNEID 190

Query: 204 PIAVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFVHADKSNLT------- 263
           PIAV V+     +   STR++S +N + +A++   I+FVI+ GF   D  NLT       
Sbjct: 191 PIAVIVVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPEN 250

Query: 264 --PFMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSIITVIYCL 323
              F PFGV GVF  AA+VY +Y G+D ++TMAEE K+P KDIP+G+ GS++I+ V+YCL
Sbjct: 251 PSGFFPFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCL 310

Query: 324 MALSLSMMQKYTDINPDAAYSVAFER-VGMKWAKYLVALGALKGMTTVLLVGALGQARYT 383
           MA+S+SM+  Y  I+ +A YS AF +  G +W   +V +GA  G+ T L+V  LGQARY 
Sbjct: 311 MAISMSMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYM 370

Query: 384 THIARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTLFVFMM 443
             I R+ ++P WFA VHPKT TP+NA+  + I    +A F+ L++L +L+S+ TLFVF M
Sbjct: 371 CVIGRSRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYM 430

Query: 444 MAVALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNGWIGYVVTVPIWF 503
           +A A++ RRY   G T     L  F+ L +I  +S+  +  W L P+G        P WF
Sbjct: 431 VANAVIFRRYVTVGYTEPWPTLS-FLCLFSI--TSILFTLVWQLAPSG-------PPKWF 490

Query: 504 LGTLGIS------------LLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFE 563
           +  LG S             ++P  R P+ WGVPL+PW P +SI  NIFL+GSL   ++ 
Sbjct: 491 I--LGASTVTAIAIVQIFHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYI 550

BLAST of Lsi02G005370 vs. TAIR 10
Match: AT5G04770.1 (cationic amino acid transporter 6 )

HSP 1 Score: 416.0 bits (1068), Expect = 5.0e-116
Identity = 234/554 (42.24%), Postives = 345/554 (62.27%), Query Frame = 0

Query: 26  SFQSWSNYGTALSQTWFRFLDRLQSRSLDENEIGELRKRSENEMKRCLTWWDLTWFGFGA 85
           SF S   Y  +LS T  R   R  S S   +E+  +R  S  +M+R L W+DL   G G 
Sbjct: 14  SFSSLRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGG 73

Query: 86  VIGAGIFVLTGQEANKHAGPAIVLSYVASGISAMLSVFCYTEFAIEIPVAGGSFAYLRIE 145
           ++GAG+FV TG+ +   AGP+IV+SY  +G+ A+LS FCYTEFA+ +PVAGG+F+Y+RI 
Sbjct: 74  MVGAGVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRIT 133

Query: 146 LGDFAAFITAGNILLESIVGTAAVARAWTSYFTSLLDRPDNSLR-IHTNLKDGFNLLDPI 205
            G+F AF T  N++++ ++  AAV+R++T+Y  +      +  R + + L  GFN +DP+
Sbjct: 134 FGEFPAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFGISTSKWRFVVSGLPKGFNEIDPV 193

Query: 206 AVAVLAIAATIAMTSTRKTSYLNWIASAVNTVVILFVIIAGFVHADKSNLTP-------- 265
           AV V+ +   I   STR++S +N I +A +   I FVI+ GF+  D  NL+         
Sbjct: 194 AVLVVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPS 253

Query: 266 -FMPFGVKGVFQAAAIVYFAYGGFDNIATMAEETKNPSKDIPLGLLGSMSIITVIYCLMA 325
            F PFG  GVF  AA+VY +Y G+D ++TMAEE +NP KDIP+G+ GS++I+TV+YCLMA
Sbjct: 254 GFFPFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMA 313

Query: 326 LSLSMMQKYTDINPDAAYSVAFE-RVGMKWAKYLVALGALKGMTTVLLVGALGQARYTTH 385
           +S+SM+  Y  I+P+A +S AF    G +W   +V +GA  G+ T LLV  LGQARY   
Sbjct: 314 VSMSMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCV 373

Query: 386 IARAHMIPPWFALVHPKTGTPINATLLITIAGGCIAFFSSLDILASLLSVSTLFVFMMMA 445
           I R+ ++P WFA +HPKT TP+NA+  + I    +A F+ L++L +L+S+ TLFVF M+A
Sbjct: 374 IGRSRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVA 433

Query: 446 VALLVRRYYARGVTPRIDQLKLFILLITIIGSSMATSAYWGLYPNG-----WIGYVVTVP 505
            AL+ RRY   G T     L  F+ L +I  +S+  +  W L P G      +G    V 
Sbjct: 434 NALIFRRYVPVGPTKPWPTL-CFLTLFSI--TSLVFTLIWKLVPEGKPKAFMLGASAVVA 493

Query: 506 IWFLGTLGISLLLPMQRKPKVWGVPLVPWLPSLSIATNIFLMGSLGPEAFERFGICTLVM 564
           I  +  L    ++P  RKP++WGVP +PW P +SI  NIFL+GSL   ++ RFG  + ++
Sbjct: 494 IAIV--LSFQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLI 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O647591.3e-24978.29Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 S... [more]
Q9SHH02.0e-20263.57Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q84MA51.1e-19763.93Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 S... [more]
Q9SQZ01.1e-11542.63Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q9LZ207.1e-11542.24Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A0A0LWU60.0e+0094.90AA_permease_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G0569... [more]
A0A6J1EAW60.0e+0094.21cationic amino acid transporter 5 OS=Cucurbita moschata OX=3662 GN=LOC111431495 ... [more]
A0A5D3BJV90.0e+0094.56Cationic amino acid transporter 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3B1F80.0e+0094.56cationic amino acid transporter 5 OS=Cucumis melo OX=3656 GN=LOC103484797 PE=4 S... [more]
A0A6J1KGQ10.0e+0094.04cationic amino acid transporter 5 OS=Cucurbita maxima OX=3661 GN=LOC111495108 PE... [more]
Match NameE-valueIdentityDescription
XP_038876967.10.0e+0096.07cationic amino acid transporter 5 [Benincasa hispida][more]
XP_004145133.20.0e+0094.90cationic amino acid transporter 5 [Cucumis sativus][more]
KGN64456.10.0e+0094.90hypothetical protein Csa_013223 [Cucumis sativus][more]
XP_022923923.10.0e+0094.21cationic amino acid transporter 5 [Cucurbita moschata][more]
KAG6584181.10.0e+0094.21Cationic amino acid transporter 5, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT2G34960.19.1e-25178.29cationic amino acid transporter 5 [more]
AT1G17120.11.4e-20363.57cationic amino acid transporter 8 [more]
AT4G21120.18.1e-19963.93amino acid transporter 1 [more]
AT3G10600.17.7e-11742.63cationic amino acid transporter 7 [more]
AT5G04770.15.0e-11642.24cationic amino acid transporter 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 73..478
e-value: 9.3E-45
score: 153.3
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 67..498
e-value: 2.0E-92
score: 312.1
NoneNo IPR availablePANTHERPTHR43243:SF22CATIONIC AMINO ACID TRANSPORTER 5coord: 10..575
NoneNo IPR availablePANTHERPTHR43243INNER MEMBRANE TRANSPORTER YGJI-RELATEDcoord: 10..575
IPR029485Cationic amino acid transporter, C-terminalPFAMPF13906AA_permease_Ccoord: 511..560
e-value: 6.9E-17
score: 61.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G005370.1Lsi02G005370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015813 L-glutamate transmembrane transport
biological_process GO:1903401 L-lysine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005313 L-glutamate transmembrane transporter activity
molecular_function GO:0015189 L-lysine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity