Lsi02G004870 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G004870
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectinesterase
Location	chr02: 4123874 .. 4124990 (-)
RNA-Seq Expression	Lsi02G004870
Synteny	Lsi02G004870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAATGTTCTTCATCAAGTTTGTAGCTGTTTGTTTGTTTGTTCTTTCATGCATGGACTTTGTTCATAGCATTTCTTTTAACACTATTGTCTCTTTAGATGGAACGAGAAATTTCAAAAGCATTGGTGCAGCTATAGCTGCTGCATCAAGCAACAACAACTCTCGATTTTACATTCATGTGAAACCTGGAATTTACAAAGAACATGTTGAAATTGAAAAGCAAAAGACTTTCATAGCGTTAATTGGAGATGATGCTGCAACTACTGTCATTGTTGATAGTAGAAGCCATGCCACTGGTTTTCGCACCTCCCAATCTGCCACATTTGGTAACAACTTTCACACTTTTTGTTTGTATTGCTAGCTTTTAAATATTAACTTTGCATTTTGATATGAAGTTACCCAAAAAAACTTTTTCTTTTGCAGTTGTGAAGGGTAATAACTTTATGGCACAGTCCATAATGTTTGAAAATTCTGCTGGACCTGAAAATGGCCAAGCTGTTGCCCTTTTCAATGACCACGCCAATTGCACGGTATACTACAAGTGTGTATTTCTTGGTTTTCAAGACACACTTTACGTGAATGGAGACAATGTATTTTTTAAAGATAGTGAAATCTACGGAACTGTAGATTTCATATTTGGATACGGACAAGCTATGTATCAAGATTGTAAGATATATGTAAGGCTAGGCAATAGAACAACTACCATAACAGCACAATCTAAACAATATCTTGACCAAAGAAGTGGCTTCGTCTTCCAAAATTGCAGTGTAACGGCCTCTCCTGAGCTCAAGTTCACAAAAAATTTGAATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCGACTGTAATTTTTATGGACTCATATTTGGACTATGTGGTTAATCCAGCCGGATGGACAGCTTGGTCAGCAGAGCGGCCTCTCAATTTATTATTCTATGCAGAATATAACAATAGAGGTCCAGGAGCTAGTTTGACTCGAAGAGTTAAATGGTCAGGCTTCCATGTGATAAATAGTCCAGCAAAAGCCATCATTTATACCGTGAATCTTTTCATTGATGGAAATAGATGGTTACCGGAAACAGGAGTACCGTTTAGATCTACCTTATAG

mRNA sequence

ATGACAATGTTCTTCATCAAGTTTGTAGCTGTTTGTTTGTTTGTTCTTTCATGCATGGACTTTGTTCATAGCATTTCTTTTAACACTATTGTCTCTTTAGATGGAACGAGAAATTTCAAAAGCATTGGTGCAGCTATAGCTGCTGCATCAAGCAACAACAACTCTCGATTTTACATTCATGTGAAACCTGGAATTTACAAAGAACATGTTGAAATTGAAAAGCAAAAGACTTTCATAGCGTTAATTGGAGATGATGCTGCAACTACTGTCATTGTTGATAGTAGAAGCCATGCCACTGGTTTTCGCACCTCCCAATCTGCCACATTTGGTAACAACTTTCACACTTTTTTTGTGAAGGGTAATAACTTTATGGCACAGTCCATAATGTTTGAAAATTCTGCTGGACCTGAAAATGGCCAAGCTGTTGCCCTTTTCAATGACCACGCCAATTGCACGGTATACTACAAGTGTGTATTTCTTGGTTTTCAAGACACACTTTACGTGAATGGAGACAATGTATTTTTTAAAGATAGTGAAATCTACGGAACTGTAGATTTCATATTTGGATACGGACAAGCTATGTATCAAGATTGTAAGATATATGTAAGGCTAGGCAATAGAACAACTACCATAACAGCACAATCTAAACAATATCTTGACCAAAGAAGTGGCTTCGTCTTCCAAAATTGCAGTGTAACGGCCTCTCCTGAGCTCAAGTTCACAAAAAATTTGAATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCGACTGTAATTTTTATGGACTCATATTTGGACTATGTGGTTAATCCAGCCGGATGGACAGCTTGGTCAGCAGAGCGGCCTCTCAATTTATTATTCTATGCAGAATATAACAATAGAGGTCCAGGAGCTAGTTTGACTCGAAGAGTTAAATGGTCAGGCTTCCATGTGATAAATAGTCCAGCAAAAGCCATCATTTATACCGTGAATCTTTTCATTGATGGAAATAGATGGTTACCGGAAACAGGAGTACCGTTTAGATCTACCTTATAG

Coding sequence (CDS)

ATGACAATGTTCTTCATCAAGTTTGTAGCTGTTTGTTTGTTTGTTCTTTCATGCATGGACTTTGTTCATAGCATTTCTTTTAACACTATTGTCTCTTTAGATGGAACGAGAAATTTCAAAAGCATTGGTGCAGCTATAGCTGCTGCATCAAGCAACAACAACTCTCGATTTTACATTCATGTGAAACCTGGAATTTACAAAGAACATGTTGAAATTGAAAAGCAAAAGACTTTCATAGCGTTAATTGGAGATGATGCTGCAACTACTGTCATTGTTGATAGTAGAAGCCATGCCACTGGTTTTCGCACCTCCCAATCTGCCACATTTGGTAACAACTTTCACACTTTTTTTGTGAAGGGTAATAACTTTATGGCACAGTCCATAATGTTTGAAAATTCTGCTGGACCTGAAAATGGCCAAGCTGTTGCCCTTTTCAATGACCACGCCAATTGCACGGTATACTACAAGTGTGTATTTCTTGGTTTTCAAGACACACTTTACGTGAATGGAGACAATGTATTTTTTAAAGATAGTGAAATCTACGGAACTGTAGATTTCATATTTGGATACGGACAAGCTATGTATCAAGATTGTAAGATATATGTAAGGCTAGGCAATAGAACAACTACCATAACAGCACAATCTAAACAATATCTTGACCAAAGAAGTGGCTTCGTCTTCCAAAATTGCAGTGTAACGGCCTCTCCTGAGCTCAAGTTCACAAAAAATTTGAATTTTGAAGTTTATCTTGGTCGACCTTGGAGAGATTATTCGACTGTAATTTTTATGGACTCATATTTGGACTATGTGGTTAATCCAGCCGGATGGACAGCTTGGTCAGCAGAGCGGCCTCTCAATTTATTATTCTATGCAGAATATAACAATAGAGGTCCAGGAGCTAGTTTGACTCGAAGAGTTAAATGGTCAGGCTTCCATGTGATAAATAGTCCAGCAAAAGCCATCATTTATACCGTGAATCTTTTCATTGATGGAAATAGATGGTTACCGGAAACAGGAGTACCGTTTAGATCTACCTTATAG

Protein sequence

MTMFFIKFVAVCLFVLSCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL

Homology

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BLAST of Lsi02G004870 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-75
Identity = 142/320 (44.38%), Postives = 205/320 (64.06%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           N +V+ DG+ N K+I  A+AAAS    SR+ I++K G Y E++E++ +   I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 88  TTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVALFND 147
            T+I  S+S   G  T +SAT         V G+NF+A+ I   N+AGP N QAVAL   
Sbjct: 262 KTIITGSKSVGGGATTFKSATVA-------VVGDNFIARDITIRNTAGPNNHQAVAL-RS 321

Query: 148 HANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRL-GN 207
            ++ +V+Y+C F G+QDTLYV+    F+++ +IYGTVDFIFG    + Q+C I+ R   N
Sbjct: 322 GSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPN 381

Query: 208 RTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDSY 267
           RT T+TAQ +   +Q +G +  NC VTA+ +LK  ++ + + +LGRPW+ YS  +++ ++
Sbjct: 382 RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQS-SVKTFLGRPWKQYSRTVYIKTF 441

Query: 268 LDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTVN 327
           LD ++NPAGW  WS +  LN L+YAEY N GPG+S   RVKW G+HV+ SP++   +TV 
Sbjct: 442 LDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVG 501

Query: 328 LFIDGNRWLPETGVPFRSTL 347
            FI GN WLP T VPF S L
Sbjct: 502 NFIAGNSWLPATNVPFTSGL 510

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BLAST of Lsi02G004870 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-72
Identity = 139/317 (43.85%), Postives = 197/317 (62.15%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           + +V+ DGT NF +I  A+ AA   +  R+ IHVK G+Y E+VEI+K+K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 88  TTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVALFND 147
            TVI  +RS   G+ T +SATF        V G  F+A+ I F+N+AGPE  QAVA+ +D
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFA-------VSGRGFIARDITFQNTAGPEKHQAVAIRSD 333

Query: 148 HANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG-- 207
             +  V+Y+C   G+QDTLY +    FF++  I GTVDFIFG   A++Q C+I  + G  
Sbjct: 334 -TDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLP 393

Query: 208 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 267
           N+  +ITAQ ++  ++ +GF  Q  ++ A  +L    N     YLGRPW+ YS  +FM +
Sbjct: 394 NQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT-ATYLGRPWKLYSRTVFMQN 453

Query: 268 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 327
           Y+   +NP GW  W+    L+ L+Y EY N GPGASL RRVKW G+HV+N+ A+A  +TV
Sbjct: 454 YMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTV 513

Query: 328 NLFIDGNRWLPETGVPF 343
           +  I GN WLP TG+ F
Sbjct: 514 SQLIQGNLWLPSTGITF 521

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BLAST of Lsi02G004870 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-71
Identity = 139/320 (43.44%), Postives = 199/320 (62.19%), Query Frame = 0


Query: 25  ISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGD 84
           +S + IV+ DGT NF ++  A+ AA   +  R+ I++K G YKE+VEI+K+K  + +IGD
Sbjct: 205 VSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGD 264

Query: 85  DAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVAL 144
               T+I  +RS   G+ T +SATF        V G  F+A+ I FEN+AGPE  QAVAL
Sbjct: 265 GMDATIISGNRSFVDGWTTFRSATFA-------VSGRGFIARDITFENTAGPEKHQAVAL 324

Query: 145 FNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRL 204
            +D ++ +V+Y+C   G+QDTLY +    F++D +I GTVDFIFG    ++Q+C+I  + 
Sbjct: 325 RSD-SDLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKK 384

Query: 205 G--NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIF 264
           G  N+  +ITAQ ++  ++ +G   Q C++TA  +L+     +   YLGRPW+ YS  + 
Sbjct: 385 GLPNQKNSITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVI 444

Query: 265 MDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAII 324
           M S+L  V+ P GW  W+ +  LN LFY EY N GPGA L  RVKW G+ V N   +A  
Sbjct: 445 MQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKN 504

Query: 325 YTVNLFIDGNRWLPETGVPF 343
           YTV  FI+GN WLP TGV +
Sbjct: 505 YTVAQFIEGNLWLPSTGVKY 516

[Close]

BLAST of Lsi02G004870 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.2e-71
Identity = 137/323 (42.41%), Postives = 196/323 (60.68%), Query Frame = 0


Query: 22  VHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIAL 81
           V   ++N  V++DGT NF +I  A+ AA + + +RF I++K G Y E+VE+ K+KT I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 82  IGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQA 141
           IGD    TVI  +RS   G+ T Q+ T G       VKG  ++A+ I F NSAGP   QA
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVG-------VKGKGYIAKDISFVNSAGPAKAQA 334

Query: 142 VALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIY 201
           VA F   ++ + +Y+C F G+QDTLYV+    F+++ +IYGT+DFIFG    ++Q+  +Y
Sbjct: 335 VA-FRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLY 394

Query: 202 VRLGN--RTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYST 261
            R  N       TAQS+   DQ +G    NC + A+P+L   K  NF+ YLGRPWR YS 
Sbjct: 395 ARKPNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSR 454

Query: 262 VIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAK 321
            + + S++D +++PAGW     +  L  L+Y EY N GPGA++ +RV W GF  I +  +
Sbjct: 455 TVIIKSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTE 514

Query: 322 AIIYTVNLFIDGNRWLPETGVPF 343
           A  +TV  FIDG+ WL  TG+PF
Sbjct: 515 ATQFTVGPFIDGSTWLNSTGIPF 528

[Close]

BLAST of Lsi02G004870 vs. ExPASy Swiss-Prot
Match: Q9M9W7 (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3702 GN=PME22 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.8e-70
Identity = 137/321 (42.68%), Postives = 199/321 (61.99%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           NT+V++DG   +++I  AI  A +++  R+ I+VK G+YKE+++++K+KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 88  TTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVALFND 147
            T+I   R+   G  T ++AT         V G  F+A+ I F N+AGP+N QAVAL  D
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVA-------VSGRGFIAKDITFRNTAGPQNRQAVALRVD 359

Query: 148 HANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVR--LG 207
            ++ + +Y+C   G+QDTLY +    F++D EIYGT+DFIFG G A+ Q+CKIY R  L 
Sbjct: 360 -SDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLP 419

Query: 208 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 267
            +  TITAQ ++  +Q +GFV QN  V A+             YLGRPW+ YS  ++M++
Sbjct: 420 LQKVTITAQGRKSPNQNTGFVIQNSYVLATQ----------PTYLGRPWKLYSRTVYMNT 479

Query: 268 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 327
           Y+  +V P GW  W     L+ L+Y EYNN GPG   + RVKW G+H+++    A+ +TV
Sbjct: 480 YMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDK-RTALSFTV 539

Query: 328 NLFIDGNRWLPETGVPFRSTL 347
             FIDG RWLP TGV F + L
Sbjct: 540 GSFIDGRRWLPATGVTFTAGL 541

[Close]

BLAST of Lsi02G004870 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 1.1e-105
Identity = 195/328 (59.45%), Postives = 246/328 (75.00%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPEN 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SA       T  V+G+NFMA+S+ FENSAGP+N
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSA-------TLTVQGDNFMAKSLTFENSAGPQN 120

Query: 139 GQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDC 198
           GQAVA+F D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+G+ M+QDC
Sbjct: 121 GQAVAVF-DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDC 180

Query: 199 KIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYS 258
            IY R+ + + T+TAQSK +L   SGF FQNC+VT SPE+   K  N +V+LGRPWR YS
Sbjct: 181 NIYARMPSNSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQ-NVKVFLGRPWRQYS 240

Query: 259 TVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPA 318
            V+FM+S+LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H+++  +
Sbjct: 241 NVVFMESFLDDVVASEGWMEWKGV-PVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKS 300

Query: 319 KAIIYTVNLFIDGNRWLPETGVPFRSTL 347
            AI +TV+ FIDG+ WL ETG+PFR  L
Sbjct: 301 -AIGFTVDNFIDGSEWLAETGIPFRRGL 317

[Close]

BLAST of Lsi02G004870 vs. ExPASy TrEMBL
Match: A0A6J1C9Z6 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.3e-103
Identity = 206/348 (59.20%), Postives = 241/348 (69.25%), Query Frame = 0


Query: 3   MFFIKFVAVCLFVLSCMDFV----HSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFY 62
           M   +FVAV    LSCM+F      +  FN  VSLDG+ ++K+I  AIAAA  N+ +RFY
Sbjct: 1   MVVFQFVAV-FVALSCMNFFVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFY 60

Query: 63  IHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFV 122
           IHV  G Y EHVEI   KTF+ALIGDDA+TT+IVD+RS+  GF+T  SATF        V
Sbjct: 61  IHVMAGTYNEHVEIPSTKTFVALIGDDASTTIIVDNRSNRIGFKTFHSATFN-------V 120

Query: 123 KGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDS 182
           KG NFMA+SI F+NSAGPENGQAVAL ND A+ TVYY C+FLGFQDTLYV GD       
Sbjct: 121 KGRNFMAKSITFKNSAGPENGQAVALLND-AHQTVYYNCIFLGFQDTLYVRGD------- 180

Query: 183 EIYGTVDFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPEL 242
                            +DCKIYV L N T TITAQSK+   + SGF FQNCSVT SPEL
Sbjct: 181 -----------------KDCKIYVGLSNTTVTITAQSKELESEHSGFSFQNCSVTTSPEL 240

Query: 243 KFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGP 302
              K+ NF+VYLGRP RDYSTVIFM S+LD VVNP GW  WS   PLNLLFYAE+NNRGP
Sbjct: 241 GPRKDQNFDVYLGRPSRDYSTVIFMQSFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGP 300

Query: 303 GASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 347
           GA +++R+KW G+H+IN+P +A  YTV  FIDG  WLPET VPFR +L
Sbjct: 301 GADISKRIKWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

[Close]

BLAST of Lsi02G004870 vs. ExPASy TrEMBL
Match: A0A5D3BM28 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004180 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 6.5e-103
Identity = 192/328 (58.54%), Postives = 242/328 (73.78%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPEN 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SA       T  V+GNNFMA+S+ FENSAGP+N
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSA-------TLTVQGNNFMAKSLTFENSAGPQN 120

Query: 139 GQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDC 198
           GQAVA+F D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+GQ M+QDC
Sbjct: 121 GQAVAVF-DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDC 180

Query: 199 KIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYS 258
            IY R+ + + T+TAQSK +L   SGF FQN +VT  PE+   K  N +V+LGR WR YS
Sbjct: 181 NIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQ-NVKVFLGRLWRQYS 240

Query: 259 TVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPA 318
            V+FM+S+LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H++N  +
Sbjct: 241 NVVFMESFLDDVVASEGWMEWKGV-PINNLFYGEFNNYGPGADVSKRVNWTGYHILNKES 300

Query: 319 KAIIYTVNLFIDGNRWLPETGVPFRSTL 347
            A  +TV+ FI+ ++WL ETG+PFR  L
Sbjct: 301 -AFRFTVDNFINRSKWLHETGIPFRHGL 317

[Close]

BLAST of Lsi02G004870 vs. ExPASy TrEMBL
Match: A0A6J1D381 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 4.2e-102
Identity = 193/340 (56.76%), Postives = 249/340 (73.24%), Query Frame = 0


Query: 8   FVAVCLFVLSCMDFVHS-ISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIY 67
           F+ +   +L  ++F H  I+FN  VSLDG+ ++  I  AIAAA +N + RFYIHVKPGIY
Sbjct: 9   FMWLVFLLLHSVEFSHCLITFNATVSLDGSGDYVHISDAIAAAPNNGSIRFYIHVKPGIY 68

Query: 68  KEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQ 127
           KEH+E+ + KTFI+LIGDDA+TTVIVD RS+  GF T+ SA       TF VK  +F+AQ
Sbjct: 69  KEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSA-------TFTVKAKHFVAQ 128

Query: 128 SIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDF 187
            + F+NSAGPENGQAVA+ ++ AN T YYKCVF+G+QDTLYV G    FK+ ++YG++DF
Sbjct: 129 FLTFQNSAGPENGQAVAVLSE-ANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDF 188

Query: 188 IFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNF 247
           IFG G A++QDC +Y R+    TTITAQSK Y DQRSGF FQNC+VT SPE++  K+   
Sbjct: 189 IFGDGLAIFQDCNVYARI--LGTTITAQSKSYKDQRSGFTFQNCNVTVSPEIQSIKD-QV 248

Query: 248 EVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRV 307
            V LGRPWR+YSTVIFM+S+LD +V P GW+ W    P+ LLFYAEYNN G GA  ++RV
Sbjct: 249 TVSLGRPWRNYSTVIFMESFLDDIVEPGGWSEW-PRTPVYLLFYAEYNNSGSGADTSQRV 308

Query: 308 KWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 347
           KW G+HV+++  +A  +TV +FIDG  WL ETG+PFR  L
Sbjct: 309 KWPGYHVLHNEDEASRFTVEIFIDGLEWLSETGIPFRRGL 336

[Close]

BLAST of Lsi02G004870 vs. ExPASy TrEMBL
Match: A0A6J1D863 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 2.3e-100
Identity = 194/348 (55.75%), Postives = 241/348 (69.25%), Query Frame = 0


Query: 1   MTMFFIKFVAVCLFVL--SCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFY 60
           MT   + F  V L V+    ++F +S +FN  VSLDG  NF  +  AIAAA + + SRFY
Sbjct: 1   MTFKVVMFFIVTLLVILDHFVEFSYSFTFNITVSLDGMGNFFRVNDAIAAAPNFSTSRFY 60

Query: 61  IHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFV 120
           IHVKPG Y EHVE+  QKTFIALIGDDA+TT+IVD+RS+ TGF T+ SA       T  V
Sbjct: 61  IHVKPGTYNEHVEVPAQKTFIALIGDDASTTIIVDNRSNGTGFSTATSA-------TLTV 120

Query: 121 KGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDS 180
            G NFMAQS+ F+NSAGP+ GQAVA+  D A  TVYYKCVFLG+QDTL+      FFK+ 
Sbjct: 121 SGENFMAQSLTFQNSAGPDKGQAVAVL-DQAKHTVYYKCVFLGYQDTLFAGAVPQFFKEC 180

Query: 181 EIYGTVDFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPEL 240
           +IYG+VDFIFG G A++QDC IY RL +   T+TAQSK  +D  SGF+FQ C+VT SPE+
Sbjct: 181 DIYGSVDFIFGNGLAIFQDCNIYARLSDIHITVTAQSKDNVDNHSGFIFQACNVTVSPEI 240

Query: 241 KFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGP 300
             +KN    V+LGRPWR YS V F++S+LD VV P GW  W    P+NLLFYAEY N G 
Sbjct: 241 VSSKN-KVTVFLGRPWRTYSMVAFIESFLDDVVQPKGWLEWPG-TPVNLLFYAEYKNSGT 300

Query: 301 GASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 347
           G+  + RV W GFH+ +  A+   +TV  FIDG +WLPETG+PFR+ L
Sbjct: 301 GSETSNRVDWPGFHLFSYAAEVANFTVENFIDGTQWLPETGIPFRTGL 338

[Close]

BLAST of Lsi02G004870 vs. NCBI nr
Match: KAA0055286.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 393.3 bits (1009), Expect = 2.2e-105
Identity = 195/328 (59.45%), Postives = 246/328 (75.00%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPEN 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SA       T  V+G+NFMA+S+ FENSAGP+N
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSA-------TLTVQGDNFMAKSLTFENSAGPQN 120

Query: 139 GQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDC 198
           GQAVA+F D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+G+ M+QDC
Sbjct: 121 GQAVAVF-DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDC 180

Query: 199 KIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYS 258
            IY R+ + + T+TAQSK +L   SGF FQNC+VT SPE+   K  N +V+LGRPWR YS
Sbjct: 181 NIYARMPSNSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQ-NVKVFLGRPWRQYS 240

Query: 259 TVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPA 318
            V+FM+S+LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H+++  +
Sbjct: 241 NVVFMESFLDDVVASEGWMEWKGV-PVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKS 300

Query: 319 KAIIYTVNLFIDGNRWLPETGVPFRSTL 347
            AI +TV+ FIDG+ WL ETG+PFR  L
Sbjct: 301 -AIGFTVDNFIDGSEWLAETGIPFRRGL 317

[Close]

BLAST of Lsi02G004870 vs. NCBI nr
Match: KAG6584281.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 391.3 bits (1004), Expect = 8.4e-105
Identity = 207/349 (59.31%), Postives = 250/349 (71.63%), Query Frame = 0


Query: 1   MTMFFIKFVAVCLFVLSCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIH 60
           M+ FFI F     FV         IS+N  VSLDG  ++ +IGAAIAAA +N++SRF IH
Sbjct: 1   MSCFFILF-----FVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIH 60

Query: 61  VKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKG 120
           VKPG+Y E+++IE  KTFI L GDDA+TT IV +RS ATGF T+ SA       TF V  
Sbjct: 61  VKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSA-------TFVVGA 120

Query: 121 NNFMAQSIMFENS--AGPENGQAVALFN-DHANCTVYYKCVFLGFQDTLYVNGDNVFFKD 180
           N+F+A SI F NS  AGP++GQAVAL N ++ N TV+YKCVFLG+QDTL++NG  +FFKD
Sbjct: 121 NHFLAVSITFSNSAGAGPKHGQAVALLNSNNENYTVFYKCVFLGYQDTLFINGKMLFFKD 180

Query: 181 SEIYGTVDFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPE 240
            EIYGTVDFIFG    ++QDC+IY RL N T T+TAQSK+Y  Q SGF FQNCSV ASPE
Sbjct: 181 CEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKEYKRQLSGFSFQNCSVMASPE 240

Query: 241 LKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRG 300
           L         V+LGRPWRDYSTVIFM+S+LD VVNPAGW+ WS    L LLFYAEY NRG
Sbjct: 241 LGPVNET--IVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRG 300

Query: 301 PGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 347
           PGA+L+ RVKW GFH I +  +A  ++V  FI+G  WLPETGVP+R  L
Sbjct: 301 PGANLSGRVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

[Close]

BLAST of Lsi02G004870 vs. NCBI nr
Match: KAG6584282.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 386.7 bits (992), Expect = 2.1e-103
Identity = 206/349 (59.03%), Postives = 247/349 (70.77%), Query Frame = 0


Query: 1   MTMFFIKFVAVCLFVLSCMDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIH 60
           M+ FFI F     FV         IS+N  VSLDG  ++ +IGAAIAAA +N++SRF IH
Sbjct: 1   MSCFFILF-----FVTFFCTTSSCISYNATVSLDGKGDYTTIGAAIAAAPNNSDSRFIIH 60

Query: 61  VKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKG 120
           VKPG+Y E++ IE  KTFI L GDDA+TT IV +RS ATGF T+ SA       TF V  
Sbjct: 61  VKPGMYFEYIRIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSA-------TFVVGA 120

Query: 121 NNFMAQSIMFENS--AGPENGQAVALFN-DHANCTVYYKCVFLGFQDTLYVNGDNVFFKD 180
           N+F+A SI F NS  AGP +GQAVAL N ++ N TV+YKCVFLG+QDTL++NG  +FFKD
Sbjct: 121 NHFLAVSITFSNSAGAGPNHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKD 180

Query: 181 SEIYGTVDFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPE 240
            EIYGTVDFIFG    ++QDC+IY RL N T T+TAQSK+   Q SGF FQNCSV ASPE
Sbjct: 181 CEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKENQLQLSGFSFQNCSVMASPE 240

Query: 241 LKFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRG 300
           L         V+LGRPWRDYSTVIFM+S+LD VVNPAGW+ WS    L LLFYAEY NRG
Sbjct: 241 LGPVNET--IVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYGNRG 300

Query: 301 PGASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 347
           PGA+L+ RVKW GFH I +  +A  ++V  FI+G  WLPETGVP+R  L
Sbjct: 301 PGANLSGRVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPYRPDL 335

[Close]

BLAST of Lsi02G004870 vs. NCBI nr
Match: XP_022137308.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 386.3 bits (991), Expect = 2.7e-103
Identity = 206/348 (59.20%), Postives = 241/348 (69.25%), Query Frame = 0


Query: 3   MFFIKFVAVCLFVLSCMDFV----HSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFY 62
           M   +FVAV    LSCM+F      +  FN  VSLDG+ ++K+I  AIAAA  N+ +RFY
Sbjct: 1   MVVFQFVAV-FVALSCMNFFVVGHGNTVFNATVSLDGSGDYKTIKDAIAAAPDNSITRFY 60

Query: 63  IHVKPGIYKEHVEIEKQKTFIALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFV 122
           IHV  G Y EHVEI   KTF+ALIGDDA+TT+IVD+RS+  GF+T  SATF        V
Sbjct: 61  IHVMAGTYNEHVEIPSTKTFVALIGDDASTTIIVDNRSNRIGFKTFHSATFN-------V 120

Query: 123 KGNNFMAQSIMFENSAGPENGQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDS 182
           KG NFMA+SI F+NSAGPENGQAVAL ND A+ TVYY C+FLGFQDTLYV GD       
Sbjct: 121 KGRNFMAKSITFKNSAGPENGQAVALLND-AHQTVYYNCIFLGFQDTLYVRGD------- 180

Query: 183 EIYGTVDFIFGYGQAMYQDCKIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPEL 242
                            +DCKIYV L N T TITAQSK+   + SGF FQNCSVT SPEL
Sbjct: 181 -----------------KDCKIYVGLSNTTVTITAQSKELESEHSGFSFQNCSVTTSPEL 240

Query: 243 KFTKNLNFEVYLGRPWRDYSTVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGP 302
              K+ NF+VYLGRP RDYSTVIFM S+LD VVNP GW  WS   PLNLLFYAE+NNRGP
Sbjct: 241 GPRKDQNFDVYLGRPSRDYSTVIFMQSFLDTVVNPKGWLEWSLGSPLNLLFYAEFNNRGP 300

Query: 303 GASLTRRVKWSGFHVINSPAKAIIYTVNLFIDGNRWLPETGVPFRSTL 347
           GA +++R+KW G+H+IN+P +A  YTV  FIDG  WLPET VPFR +L
Sbjct: 301 GADISKRIKWQGYHLINNPLEATKYTVKDFIDGTTWLPETNVPFRPSL 315

[Close]

BLAST of Lsi02G004870 vs. NCBI nr
Match: TYJ99208.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 384.0 bits (985), Expect = 1.3e-102
Identity = 192/328 (58.54%), Postives = 242/328 (73.78%), Query Frame = 0


Query: 19  MDFVHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTF 78
           M FVHSISFN  VSLDG+ NFK I  AI AA +N+N+RFYIHV PG Y E ++I K K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPEN 138
           IALIGDDA TT+I+D+RS+ TGF+T+ SA       T  V+GNNFMA+S+ FENSAGP+N
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSA-------TLTVQGNNFMAKSLTFENSAGPQN 120

Query: 139 GQAVALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDC 198
           GQAVA+F D A+ T YYKC FLGFQDTLYVNG   FFK+S IYG+VDFIFG+GQ M+QDC
Sbjct: 121 GQAVAVF-DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDC 180

Query: 199 KIYVRLGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYS 258
            IY R+ + + T+TAQSK +L   SGF FQN +VT  PE+   K  N +V+LGR WR YS
Sbjct: 181 NIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQ-NVKVFLGRLWRQYS 240

Query: 259 TVIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPA 318
            V+FM+S+LD VV   GW  W    P+N LFY E+NN GPGA +++RV W+G+H++N  +
Sbjct: 241 NVVFMESFLDDVVASEGWMEWKGV-PINNLFYGEFNNYGPGADVSKRVNWTGYHILNKES 300

Query: 319 KAIIYTVNLFIDGNRWLPETGVPFRSTL 347
            A  +TV+ FI+ ++WL ETG+PFR  L
Sbjct: 301 -AFRFTVDNFINRSKWLHETGIPFRHGL 317

[Close]

BLAST of Lsi02G004870 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 275.0 bits (702), Expect = 8.2e-74
Identity = 139/317 (43.85%), Postives = 197/317 (62.15%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           + +V+ DGT NF +I  A+ AA   +  R+ IHVK G+Y E+VEI+K+K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 88  TTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVALFND 147
            TVI  +RS   G+ T +SATF        V G  F+A+ I F+N+AGPE  QAVA+ +D
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFA-------VSGRGFIARDITFQNTAGPEKHQAVAIRSD 333

Query: 148 HANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG-- 207
             +  V+Y+C   G+QDTLY +    FF++  I GTVDFIFG   A++Q C+I  + G  
Sbjct: 334 -TDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLP 393

Query: 208 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 267
           N+  +ITAQ ++  ++ +GF  Q  ++ A  +L    N     YLGRPW+ YS  +FM +
Sbjct: 394 NQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT-ATYLGRPWKLYSRTVFMQN 453

Query: 268 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 327
           Y+   +NP GW  W+    L+ L+Y EY N GPGASL RRVKW G+HV+N+ A+A  +TV
Sbjct: 454 YMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTV 513

Query: 328 NLFIDGNRWLPETGVPF 343
           +  I GN WLP TG+ F
Sbjct: 514 SQLIQGNLWLPSTGITF 521

[Close]

BLAST of Lsi02G004870 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 268.9 bits (686), Expect = 5.8e-72
Identity = 137/323 (42.41%), Postives = 196/323 (60.68%), Query Frame = 0


Query: 22  VHSISFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIAL 81
           V   ++N  V++DGT NF +I  A+ AA + + +RF I++K G Y E+VE+ K+KT I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 82  IGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQA 141
           IGD    TVI  +RS   G+ T Q+ T G       VKG  ++A+ I F NSAGP   QA
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVG-------VKGKGYIAKDISFVNSAGPAKAQA 334

Query: 142 VALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIY 201
           VA F   ++ + +Y+C F G+QDTLYV+    F+++ +IYGT+DFIFG    ++Q+  +Y
Sbjct: 335 VA-FRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLY 394

Query: 202 VRLGN--RTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYST 261
            R  N       TAQS+   DQ +G    NC + A+P+L   K  NF+ YLGRPWR YS 
Sbjct: 395 ARKPNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE-NFKAYLGRPWRKYSR 454

Query: 262 VIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAK 321
            + + S++D +++PAGW     +  L  L+Y EY N GPGA++ +RV W GF  I +  +
Sbjct: 455 TVIIKSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTE 514

Query: 322 AIIYTVNLFIDGNRWLPETGVPF 343
           A  +TV  FIDG+ WL  TG+PF
Sbjct: 515 ATQFTVGPFIDGSTWLNSTGIPF 528

[Close]

BLAST of Lsi02G004870 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 137/321 (42.68%), Postives = 199/321 (61.99%), Query Frame = 0


Query: 28  NTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDDAA 87
           NT+V++DG   +++I  AI  A +++  R+ I+VK G+YKE+++++K+KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 88  TTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVALFND 147
            T+I   R+   G  T ++AT         V G  F+A+ I F N+AGP+N QAVAL  D
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVA-------VSGRGFIAKDITFRNTAGPQNRQAVALRVD 359

Query: 148 HANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVR--LG 207
            ++ + +Y+C   G+QDTLY +    F++D EIYGT+DFIFG G A+ Q+CKIY R  L 
Sbjct: 360 -SDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLP 419

Query: 208 NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFMDS 267
            +  TITAQ ++  +Q +GFV QN  V A+             YLGRPW+ YS  ++M++
Sbjct: 420 LQKVTITAQGRKSPNQNTGFVIQNSYVLATQ----------PTYLGRPWKLYSRTVYMNT 479

Query: 268 YLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIYTV 327
           Y+  +V P GW  W     L+ L+Y EYNN GPG   + RVKW G+H+++    A+ +TV
Sbjct: 480 YMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIMDK-RTALSFTV 539

Query: 328 NLFIDGNRWLPETGVPFRSTL 347
             FIDG RWLP TGV F + L
Sbjct: 540 GSFIDGRRWLPATGVTFTAGL 541

[Close]

BLAST of Lsi02G004870 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 267.3 bits (682), Expect = 1.7e-71
Identity = 140/323 (43.34%), Postives = 204/323 (63.16%), Query Frame = 0


Query: 26  SFNTIVSLDGTRNFKSIGAAIAAASSNNNSRFYIHVKPGIYKEHVEIEKQKTFIALIGDD 85
           +++  V+LDGT NF  I  AI  A   +++RF I++K G+Y E+VEI+K+K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 86  AATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQAVALF 145
              TVI  +RS   G+ T +SATF        V G  F+A+ I F+N+AGPE  QAVAL 
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFA-------VSGRGFLARDITFQNTAGPEKHQAVALR 329

Query: 146 NDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIYVRLG 205
           +D ++ +V+++C   G+QDTLY +    F+++  I GTVDFIFG G  ++Q+C+I  + G
Sbjct: 330 SD-SDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRG 389

Query: 206 --NRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYSTVIFM 265
             N+  TITAQ ++ ++Q SGF  Q  +++A  +L    N     YLGRPW+ YS  +F+
Sbjct: 390 LPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTT-RTYLGRPWKLYSRTVFI 449

Query: 266 DSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAKAIIY 325
            + +  VV P GW  W+A+  L+ LFY E+ N GPG+ L+ RVKW G+HV N+  +A  +
Sbjct: 450 RNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNF 509

Query: 326 TVNLFIDGNRWLPETGVPFRSTL 347
           TV+ FI GN WLP TGV F   L
Sbjct: 510 TVSQFIKGNLWLPSTGVTFSDGL 523

[Close]

BLAST of Lsi02G004870 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 265.8 bits (678), Expect = 4.9e-71
Identity = 139/327 (42.51%), Postives = 199/327 (60.86%), Query Frame = 0


Query: 25  ISFNTIVSLDGTRNFKSIGAAIAAASSNNNSR---FYIHVKPGIYKEHVEIEKQKTFIAL 84
           IS    VS DGT NF +I AA+AAA +N +     F I+V  GIY+E++ I K K ++ +
Sbjct: 261 ISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMM 320

Query: 85  IGDDAATTVIVDSRSHATGFRTSQSATFGNNFHTFFVKGNNFMAQSIMFENSAGPENGQA 144
           IGD    TV+  +RS   G+ T  SATF        V   NF+A +I F N+AGPE  QA
Sbjct: 321 IGDGINQTVVTGNRSVVDGWTTFNSATFA-------VTAPNFVAVNITFRNTAGPEKHQA 380

Query: 145 VALFNDHANCTVYYKCVFLGFQDTLYVNGDNVFFKDSEIYGTVDFIFGYGQAMYQDCKIY 204
           VAL    A+ +++Y C F  +QDTLY +    F+++ ++YGTVDFIFG    ++Q+C +Y
Sbjct: 381 VAL-RSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLY 440

Query: 205 VR--LGNRTTTITAQSKQYLDQRSGFVFQNCSVTASPELKFTKNLNFEVYLGRPWRDYST 264
            R  + N+   ITAQ +   +Q +G   QNC++  + +L  + N   + YLGRPW++YS 
Sbjct: 441 PRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTYLGRPWKEYSR 500

Query: 265 VIFMDSYLDYVVNPAGWTAWSAERPLNLLFYAEYNNRGPGASLTRRVKWSGFHVINSPAK 324
            ++M SY+D  V P GW  W+ +  L+ L+YAEYNN GPG++ T RV W G+HVINS   
Sbjct: 501 TVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDA 560

Query: 325 AIIYTVNLFIDGNRWLPETGVPFRSTL 347
           A      LFI+ + W+ +TGVP+ S L
Sbjct: 561 ANFTVTGLFIEAD-WIWKTGVPYTSGL 577

The following BLAST results are available for this feature:

BLAST of Lsi02G004870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	1.1e-75	44.38	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9LXK7	1.1e-72	43.85	Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3...	[more]
Q43062	1.7e-71	43.44	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]
O81415	8.2e-71	42.41	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
Q9M9W7	1.8e-70	42.68	Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Lsi02G004870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7ULX7	1.1e-105	59.45	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290...	[more]
A0A6J1C9Z6	1.3e-103	59.20	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008805 PE=4 SV=1	[more]
A0A5D3BM28	6.5e-103	58.54	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00418...	[more]
A0A6J1D381	4.2e-102	56.76	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1	[more]
A0A6J1D863	2.3e-100	55.75	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1	[more]

BLAST of Lsi02G004870 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0055286.1	2.2e-105	59.45	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]
KAG6584281.1	8.4e-105	59.31	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG6584282.1	2.1e-103	59.03	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
XP_022137308.1	2.7e-103	59.20	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
TYJ99208.1	1.3e-102	58.54	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi02G004870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G43270.1	8.2e-74	43.85	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	5.8e-72	42.41	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05620.1	1.3e-71	42.68	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33220.1	1.7e-71	43.34	pectin methylesterase 44	[more]
AT1G02810.1	4.9e-71	42.51	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 23..346 e-value: 3.0E-107 score: 360.7
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 29..332 e-value: 1.9E-99 score: 332.1
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 23..344
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 23..344
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..18 score: 5.0
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 53..72
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 180..189
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 26..344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G004870.1	Lsi02G004870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
cellular_component	GO:0016020	membrane
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity