Lsi02G004480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G004480
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectinesterase
Location	chr02: 3743999 .. 3745470 (+)
RNA-Seq Expression	Lsi02G004480
Synteny	Lsi02G004480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATTTTCAAGGTTGTTGCAATTTTACTCTTTTGCTTCTTCAACCTCATGATGATATCTGTTCATTCCATTAATTTTGATGTAATGGTATCGTTAGATGGTTCAAAAGATTTCAAAAGTATTAGTGAAGCCATTGCAACAGCTCCTGACAATTCTAATATACGATTTTACATTCATGTAACTCCTGGAACTTACCATGAACGTCTTCAAATTCCAAAAACCAAGACCTTCATTGCTTTGGTCGGTGATAATGCTTTAACAACTATTATCGTCGACAATAGAAGCAACCGTCATGGATTCAAGACCACTGATTCAGCAACTCTTAGTAAGTGTTTGTCTAAATTTTATTTTCTTTTTATTCCATTATAGCATTTTTTATTCATCAATATTCACACTCGCACGTCTTTTTTATATCTTCTGAGGTTTTGTTTTTGCTAACTTGACTTTCTTTTTCAATTTGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTTTCAAAAGTCGTGCTTAGAATAATTTGGACTTAAATGTTTGAGAAAAAAAATTGTGTGATTGAAAACAATAGTTCCATAGGACCTTTTACTTATGATCAAAGTGTCGGATTTAAAAATGAGTTCATGGGGAGAGATGGTTTAAATTGGGTTTTTGTATATTGAACTCTATTGTTGGATAAGGCAGAGAGTATTATTGTTAAATTATTAATTTGAAGTCTGGTATACATTATAATTAGGCTTTTCACTTATGCATACTATTAATTTAAGCATTTTCATTTGATGTAGCGGTTGACGGTGACAACTTTTTAGCACAGTCGTTGACCTTTGAGAACTCTGCCGGTCCTCAAAATGGCCAAGCTGTGGCAGTCCTTGACCAAGCTGACCACACTGCCTATCACAAGTGTAGATTTTTGGGTTTCCAAGACACCCTATACGTCAAAGGACGACCCCAATTCTTCAAGGAGTGTGACATCTACGGAAGCGTAGACTTCATCTTTGGACATGGGCTAGTTATGTTCCAAGACTGTAACATATATGCTCGAATACCAAACAAATCTAACACAGTCACCGCTCAATCTAAAGAAAGATCACGAGATGTGAGTGGCTTTTCATTTCAAAACTGTACCGTTACAGTTTCATCAGAGATAGCAGCCAATAAAGCCAGCGTTCAAATTTATTTGGGTCGGCCATGGAGACAGTACTCAAAGGTTGTGTTCATGGAGTCATTTCTCGATGACGTAGTGGCGCCAGAAGGATGGTCGGAGTGGTCAGGAGTTTCAATCAATAATTTGTTTTATGCAGAGTTCAACAACCGCGGTCCTGGGGCTGATGTCTCCAAAAGGGTTAATTGGACAACTTTTCATATATTAGATAAACAATTTGCCATTCAGTTTACTGTAGATAACTTTATTAATGCATCTGAATGGTTGCCCGAAACTGGCGTTCCATTTAGACGTGGACTTCTTTTATGA

mRNA sequence

ATGGAAATTTTCAAGGTTGTTGCAATTTTACTCTTTTGCTTCTTCAACCTCATGATGATATCTGTTCATTCCATTAATTTTGATGTAATGGTATCGTTAGATGGTTCAAAAGATTTCAAAAGTATTAGTGAAGCCATTGCAACAGCTCCTGACAATTCTAATATACGATTTTACATTCATGTAACTCCTGGAACTTACCATGAACGTCTTCAAATTCCAAAAACCAAGACCTTCATTGCTTTGGTCGGTGATAATGCTTTAACAACTATTATCGTCGACAATAGAAGCAACCGTCATGGATTCAAGACCACTGATTCAGCAACTCTTACGGTTGACGGTGACAACTTTTTAGCACAGTCGTTGACCTTTGAGAACTCTGCCGGTCCTCAAAATGGCCAAGCTGTGGCAGTCCTTGACCAAGCTGACCACACTGCCTATCACAAGTGTAGATTTTTGGGTTTCCAAGACACCCTATACGTCAAAGGACGACCCCAATTCTTCAAGGAGTGTGACATCTACGGAAGCGTAGACTTCATCTTTGGACATGGGCTAGTTATGTTCCAAGACTGTAACATATATGCTCGAATACCAAACAAATCTAACACAGTCACCGCTCAATCTAAAGAAAGATCACGAGATGTGAGTGGCTTTTCATTTCAAAACTGTACCGTTACAGTTTCATCAGAGATAGCAGCCAATAAAGCCAGCGTTCAAATTTATTTGGGTCGGCCATGGAGACAGTACTCAAAGGTTGTGTTCATGGAGTCATTTCTCGATGACGTAGTGGCGCCAGAAGGATGGTCGGAGTGGTCAGGAGTTTCAATCAATAATTTGTTTTATGCAGAGTTCAACAACCGCGGTCCTGGGGCTGATGTCTCCAAAAGGGTTAATTGGACAACTTTTCATATATTAGATAAACAATTTGCCATTCAGTTTACTGTAGATAACTTTATTAATGCATCTGAATGGTTGCCCGAAACTGGCGTTCCATTTAGACGTGGACTTCTTTTATGA

Coding sequence (CDS)

ATGGAAATTTTCAAGGTTGTTGCAATTTTACTCTTTTGCTTCTTCAACCTCATGATGATATCTGTTCATTCCATTAATTTTGATGTAATGGTATCGTTAGATGGTTCAAAAGATTTCAAAAGTATTAGTGAAGCCATTGCAACAGCTCCTGACAATTCTAATATACGATTTTACATTCATGTAACTCCTGGAACTTACCATGAACGTCTTCAAATTCCAAAAACCAAGACCTTCATTGCTTTGGTCGGTGATAATGCTTTAACAACTATTATCGTCGACAATAGAAGCAACCGTCATGGATTCAAGACCACTGATTCAGCAACTCTTACGGTTGACGGTGACAACTTTTTAGCACAGTCGTTGACCTTTGAGAACTCTGCCGGTCCTCAAAATGGCCAAGCTGTGGCAGTCCTTGACCAAGCTGACCACACTGCCTATCACAAGTGTAGATTTTTGGGTTTCCAAGACACCCTATACGTCAAAGGACGACCCCAATTCTTCAAGGAGTGTGACATCTACGGAAGCGTAGACTTCATCTTTGGACATGGGCTAGTTATGTTCCAAGACTGTAACATATATGCTCGAATACCAAACAAATCTAACACAGTCACCGCTCAATCTAAAGAAAGATCACGAGATGTGAGTGGCTTTTCATTTCAAAACTGTACCGTTACAGTTTCATCAGAGATAGCAGCCAATAAAGCCAGCGTTCAAATTTATTTGGGTCGGCCATGGAGACAGTACTCAAAGGTTGTGTTCATGGAGTCATTTCTCGATGACGTAGTGGCGCCAGAAGGATGGTCGGAGTGGTCAGGAGTTTCAATCAATAATTTGTTTTATGCAGAGTTCAACAACCGCGGTCCTGGGGCTGATGTCTCCAAAAGGGTTAATTGGACAACTTTTCATATATTAGATAAACAATTTGCCATTCAGTTTACTGTAGATAACTTTATTAATGCATCTGAATGGTTGCCCGAAACTGGCGTTCCATTTAGACGTGGACTTCTTTTATGA

Protein sequence

MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASEWLPETGVPFRRGLLL

Homology

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BLAST of Lsi02G004480 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.8e-78
Identity = 142/311 (45.66%), Postives = 208/311 (66.88%), Query Frame = 0


Query: 28  DVMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTKTFIALVGDNAL 87
           +++V+ DGS + K+I EA+A A      R+ I++  GTY+E +++ K K  I  VGD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEV-KLKN-IMFVGDGIG 261

Query: 88  TTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTAYH 147
            TII  ++S   G  T  SAT+ V GDNF+A+ +T  N+AGP N QAVA+   +D + ++
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 148 KCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYAR-IPNKSNTVTAQ 207
           +C F G+QDTLYV  + QF++ECDIYG+VDFIFG+  V+ Q+CNI+AR  PN++NT+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 208 SKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVAPEG 267
            +      +G    NC VT +S++   ++SV+ +LGRPW+QYS+ V++++FLD ++ P G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 268 WSEWSG-VSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAI-QFTVDNFINASEWL 327
           W EWSG  ++N L+YAE+ N GPG+  + RV W  +H+L     + QFTV NFI  + WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 328 PETGVPFRRGL 336
           P T VPF  GL
Sbjct: 502 PATNVPFTSGL 510

[Close]

BLAST of Lsi02G004480 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 132/314 (42.04%), Postives = 207/314 (65.92%), Query Frame = 0


Query: 29  VMVSLDGSKDFKSISEAIATAPDNSNIR---FYIHVTPGTYHERLQIPKTKTFIALVGDN 88
           V VS DG+ +F +I+ A+A AP+N++     F I+VT G Y E + I K K ++ ++GD 
Sbjct: 265 VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 324

Query: 89  ALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTA 148
              T++  NRS   G+ T +SAT  V   NF+A ++TF N+AGP+  QAVA+   AD + 
Sbjct: 325 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 384

Query: 149 YHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYAR--IPNKSNTV 208
           ++ C F  +QDTLY     QF++ECD+YG+VDFIFG+  V+FQ+CN+Y R  +PN+ N +
Sbjct: 385 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 444

Query: 209 TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVA 268
           TAQ +      +G S QNCT+  + ++ ++  +V+ YLGRPW++YS+ V+M+S++D  V 
Sbjct: 445 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 504

Query: 269 PEGWSEWSG-VSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASE 328
           P GW EW+G  +++ L+YAE+NN GPG++ + RV W  +H+++   A  FTV      ++
Sbjct: 505 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEAD 564

Query: 329 WLPETGVPFRRGLL 337
           W+ +TGVP+  GL+
Sbjct: 565 WIWKTGVPYTSGLI 578

[Close]

BLAST of Lsi02G004480 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 9.8e-77
Identity = 136/322 (42.24%), Postives = 208/322 (64.60%), Query Frame = 0


Query: 17  LMMISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTK 76
           L+   V   N+++ V++DG+ +F +I++A+  AP+ S  RF I++  G Y E +++PK K
Sbjct: 210 LLEAPVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKK 269

Query: 77  TFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVA 136
           T I  +GD    T+I  NRS   G+ T  + T+ V G  ++A+ ++F NSAGP   QAVA
Sbjct: 270 TMIMFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA 329

Query: 137 VLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARI 196
               +DH+A+++C F G+QDTLYV    QF++ECDIYG++DFIFG+  V+FQ+ ++YAR 
Sbjct: 330 FRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARK 389

Query: 197 PNKSNTV--TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFM 256
           PN  + +  TAQS+ +S   +G S  NC +  + ++   K + + YLGRPWR+YS+ V +
Sbjct: 390 PNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVII 449

Query: 257 ESFLDDVVAPEGWSEW-SGVSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQF-AIQF 316
           +SF+DD++ P GW E     ++  L+Y E+ N GPGA+++KRV W  F  ++ Q  A QF
Sbjct: 450 KSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQF 509

Query: 317 TVDNFINASEWLPETGVPFRRG 335
           TV  FI+ S WL  TG+PF  G
Sbjct: 510 TVGPFIDGSTWLNSTGIPFSLG 531

[Close]

BLAST of Lsi02G004480 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 9.8e-77
Identity = 133/314 (42.36%), Postives = 206/314 (65.61%), Query Frame = 0


Query: 29  VMVSLDGSKDFKSISEAIATAP---DNSNIRFYIHVTPGTYHERLQIPKTKTFIALVGDN 88
           V V+ +G+ +F +I+EA+ +AP   D +   F I+VT G Y E + I K K ++ ++GD 
Sbjct: 259 VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 318

Query: 89  ALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTA 148
              T++  NR+   G+ T +SAT  V   NF+A ++TF N+AGP+  QAVA+   AD + 
Sbjct: 319 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 378

Query: 149 YHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIP--NKSNTV 208
           ++ C F  +QDTLY     QF++ECDIYG+VDFIFG+  V+FQDCN+Y R P  N+ N +
Sbjct: 379 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 438

Query: 209 TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVA 268
           TAQ +      +G S  NCT+  + ++ ++  +V+ YLGRPW++YS+ VFM+S++D+VV 
Sbjct: 439 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 498

Query: 269 PEGWSEWSG-VSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASE 328
           P GW EW+G  +++ L+YAE+NN G G+  + RV W  +H+++   A  FTV+NF+    
Sbjct: 499 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 558

Query: 329 WLPETGVPFRRGLL 337
           W+ ++GVP+  GLL
Sbjct: 559 WMVQSGVPYISGLL 572

[Close]

BLAST of Lsi02G004480 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-76
Identity = 133/314 (42.36%), Postives = 201/314 (64.01%), Query Frame = 0


Query: 29  VMVSLDGSKDFKSISEAIATAP---DNSNIRFYIHVTPGTYHERLQIPKTKTFIALVGDN 88
           V V  +G+ +F +I+ AIA AP   D SN  F I+VT G Y E +++PK K ++ ++GD 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 89  ALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTA 148
              T+I  NRS   G+ T +SAT  + G NF+  ++T  N+AGP  GQAVA+    D + 
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 149 YHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIPNK--SNTV 208
           ++ C F  +QDTLY     QF++ECD+YG+VDFIFG+  V+ Q+CN+Y R P K  SN V
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEV 425

Query: 209 TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVA 268
           TAQ +      +G +   CT+  + ++A +  +V+ YLGRPW++YS+ V M++++D  + 
Sbjct: 426 TAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLE 485

Query: 269 PEGWSEWSG-VSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASE 328
           P GW+ WSG  +++ L+YAE+NN GPG+D + RV W  +H+++   A  FTV NF+    
Sbjct: 486 PSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEG 545

Query: 329 WLPETGVPFRRGLL 337
           W+ +TGVPF  GL+
Sbjct: 546 WIGQTGVPFVGGLI 559

[Close]

BLAST of Lsi02G004480 vs. ExPASy TrEMBL
Match: A0A5D3BKF8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004210 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.4e-150
Identity = 267/336 (79.46%), Postives = 295/336 (87.80%), Query Frame = 0


Query: 1   MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGSKD-FKSISEAIATAPDNSNIRFYI 60
           ME  K V I + CFFNL+   +HS  F+  VSLDGS + FK+I+ AIA APDNSN RFYI
Sbjct: 1   MENLKAVVITVMCFFNLVPF-IHSFTFNATVSLDGSMNHFKTIAAAIAAAPDNSNTRFYI 60

Query: 61  HVTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQ 120
            VTPGTYHERLQIP TKTFIAL+GDNALTTIIVD+RSN  GFKT+DSATLTV+G+NFLA+
Sbjct: 61  RVTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGNNFLAK 120

Query: 121 SLTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFI 180
           SLTFENSAGPQNGQAVAVLDQA  TAYHKC+FLGFQDTLYVKG  QFFKEC+IYGSVDFI
Sbjct: 121 SLTFENSAGPQNGQAVAVLDQAHFTAYHKCKFLGFQDTLYVKG-TQFFKECEIYGSVDFI 180

Query: 181 FGHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQI 240
           FG+GLVMFQDCNIYAR PN+  TVTAQSKE+ ++VSGFSFQNCTVTVSSEIA NKA+V+I
Sbjct: 181 FGNGLVMFQDCNIYAREPNRPITVTAQSKEQPKEVSGFSFQNCTVTVSSEIAVNKANVKI 240

Query: 241 YLGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWT 300
           YLGRPWRQYSKVVFMESFLDD V PEGWSEW GV +NNLFYAEFNNRGPGADVSKRV+WT
Sbjct: 241 YLGRPWRQYSKVVFMESFLDDEVVPEGWSEWPGVPVNNLFYAEFNNRGPGADVSKRVHWT 300

Query: 301 TFHILDKQFAIQFTVDNFINASEWLPETGVPFRRGL 336
            F +LDKQ A QFTVDNF+N S+WLPETG+PFR GL
Sbjct: 301 GFRVLDKQSAYQFTVDNFVNGSKWLPETGIPFRDGL 334

[Close]

BLAST of Lsi02G004480 vs. ExPASy TrEMBL
Match: A0A5D3BHF7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004200 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.4e-147
Identity = 258/335 (77.01%), Postives = 288/335 (85.97%), Query Frame = 0


Query: 1   MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGS-KDFKSISEAIATAPDNSNIRFYI 60
           MEI K V I + CFFNL+   +HS  F+  VSLDGS  DFK+I+ AIA APDNSN RFYI
Sbjct: 1   MEILKAVVITVMCFFNLVPF-IHSFTFNATVSLDGSMNDFKTIAAAIAAAPDNSNTRFYI 60

Query: 61  HVTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQ 120
            VTPGTYHERLQIP TKTFIAL+GDNALTTIIVD+RSN  GFKT+DSATLTV+GDNF+A+
Sbjct: 61  RVTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGDNFIAK 120

Query: 121 SLTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFI 180
           SLTFENSAGPQNGQAVAVLDQA  TAYHKCRFLGFQDTLYV G+  FFKECD+YG+VDFI
Sbjct: 121 SLTFENSAGPQNGQAVAVLDQARFTAYHKCRFLGFQDTLYVGGKSHFFKECDVYGTVDFI 180

Query: 181 FGHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQI 240
           FG+GL MFQDCNIYAR PN+  TVTAQSKE+  + SGFSFQNCTVTVSSEI A+KA+V+I
Sbjct: 181 FGNGLTMFQDCNIYAREPNRPITVTAQSKEQLHEESGFSFQNCTVTVSSEIVASKANVKI 240

Query: 241 YLGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWT 300
           YL RPWRQYS+VVFMESFLD+ V PEGWSEW GV  NNLFY EFNNRGPGADVSKRV+W 
Sbjct: 241 YLDRPWRQYSRVVFMESFLDEEVLPEGWSEWPGVPTNNLFYGEFNNRGPGADVSKRVHWI 300

Query: 301 TFHILDKQFAIQFTVDNFINASEWLPETGVPFRRG 335
            FH+L+KQ A QFT+DNF+N S WLPETG+PFRRG
Sbjct: 301 GFHLLNKQLANQFTIDNFVNGSYWLPETGIPFRRG 334

[Close]

BLAST of Lsi02G004480 vs. ExPASy TrEMBL
Match: A0A0A0LR14 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.4e-144
Identity = 250/335 (74.63%), Postives = 288/335 (85.97%), Query Frame = 0


Query: 1   MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIH 60
           M  F  V   LFCFFNLM++ +HS++F++ VSLDGS +FK IS+AIA AP+NSN RFYIH
Sbjct: 1   MLFFFGVPCSLFCFFNLMLL-IHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIH 60

Query: 61  VTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQS 120
           V PGTYHE LQIP+ K FIALVGD+  TTII+DNRSN  GFKT +SATLTV GDNF+A+S
Sbjct: 61  VAPGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAES 120

Query: 121 LTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIF 180
           LTFENSAGPQN QAVAV D+A+HTAY+KCRFL FQDTLYV G+PQFFKE DIYGSVDFI 
Sbjct: 121 LTFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFIC 180

Query: 181 GHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIY 240
           G+G VMFQDCNIYAR+P  S TVTAQSK   R VSGFSFQNCTVTVS EI++NK +V+++
Sbjct: 181 GYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVF 240

Query: 241 LGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWTT 300
           LGRPW+QYSKVVFMESFLDDVVA EGW EW GV +NNLFY EFNN GPGADVSKRVNWT+
Sbjct: 241 LGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTS 300

Query: 301 FHILDKQFAIQFTVDNFINASEWLPETGVPFRRGL 336
           +H+LDK+ A++FTVDNF+N SEWLPETG+PFRRGL
Sbjct: 301 YHLLDKESALRFTVDNFVNGSEWLPETGIPFRRGL 334

[Close]

BLAST of Lsi02G004480 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.2e-143
Identity = 244/318 (76.73%), Postives = 278/318 (87.42%), Query Frame = 0


Query: 19  MISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTKTF 78
           M+ VHSI+F++ VSLDGS +FK IS+AI  AP+NSN RFYIHV PGTYHE +QIPK K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVL 138
           IAL+GD+A TTII+DNRSN  GFKT +SATLTV GDNF+A+SLTFENSAGPQNGQAVAV 
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 DQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIPN 198
           D+ADHTAY+KCRFLGFQDTLYV G+ QFFKE +IYGSVDFIFGHG VMFQDCNIYAR+P+
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 199 KSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFL 258
            S TVTAQSK+  R +SGFSFQNCTVTVS EI++NK +V+++LGRPWRQYS VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 259 DDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFI 318
           DDVVA EGW EW GV +NNLFY EFNN GPGADVSKRVNWT +H+LDK+ AI FTVDNFI
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 319 NASEWLPETGVPFRRGLL 337
           + SEWL ETG+PFRRGLL
Sbjct: 301 DGSEWLAETGIPFRRGLL 318

[Close]

BLAST of Lsi02G004480 vs. ExPASy TrEMBL
Match: A0A5D3BM28 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004180 PE=4 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 2.1e-138
Identity = 239/318 (75.16%), Postives = 274/318 (86.16%), Query Frame = 0


Query: 19  MISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTKTF 78
           M+ VHSI+F++ VSLDGS +FK IS+AI  AP+NSN RFYIHV PGTYHE +QIPK K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVL 138
           IAL+GD+A TTII+DNRSN  GFKT +SATLTV G+NF+A+SLTFENSAGPQNGQAVAV 
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 DQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIPN 198
           D+ADHTAY+KCRFLGFQDTLYV G+ QFFKE +IYGSVDFIFGHG VMFQDCNIYAR+P+
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMPS 180

Query: 199 KSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFL 258
            S TVTAQSK+  R +SGFSFQN TVTV  EI++NK +V+++LGR WRQYS VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFL 240

Query: 259 DDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFI 318
           DDVVA EGW EW GV INNLFY EFNN GPGADVSKRVNWT +HIL+K+ A +FTVDNFI
Sbjct: 241 DDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFI 300

Query: 319 NASEWLPETGVPFRRGLL 337
           N S+WL ETG+PFR GLL
Sbjct: 301 NRSKWLHETGIPFRHGLL 318

[Close]

BLAST of Lsi02G004480 vs. NCBI nr
Match: KAA0055288.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >TYJ99211.1 putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 541.6 bits (1394), Expect = 4.9e-150
Identity = 267/336 (79.46%), Postives = 295/336 (87.80%), Query Frame = 0


Query: 1   MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGSKD-FKSISEAIATAPDNSNIRFYI 60
           ME  K V I + CFFNL+   +HS  F+  VSLDGS + FK+I+ AIA APDNSN RFYI
Sbjct: 1   MENLKAVVITVMCFFNLVPF-IHSFTFNATVSLDGSMNHFKTIAAAIAAAPDNSNTRFYI 60

Query: 61  HVTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQ 120
            VTPGTYHERLQIP TKTFIAL+GDNALTTIIVD+RSN  GFKT+DSATLTV+G+NFLA+
Sbjct: 61  RVTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGNNFLAK 120

Query: 121 SLTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFI 180
           SLTFENSAGPQNGQAVAVLDQA  TAYHKC+FLGFQDTLYVKG  QFFKEC+IYGSVDFI
Sbjct: 121 SLTFENSAGPQNGQAVAVLDQAHFTAYHKCKFLGFQDTLYVKG-TQFFKECEIYGSVDFI 180

Query: 181 FGHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQI 240
           FG+GLVMFQDCNIYAR PN+  TVTAQSKE+ ++VSGFSFQNCTVTVSSEIA NKA+V+I
Sbjct: 181 FGNGLVMFQDCNIYAREPNRPITVTAQSKEQPKEVSGFSFQNCTVTVSSEIAVNKANVKI 240

Query: 241 YLGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWT 300
           YLGRPWRQYSKVVFMESFLDD V PEGWSEW GV +NNLFYAEFNNRGPGADVSKRV+WT
Sbjct: 241 YLGRPWRQYSKVVFMESFLDDEVVPEGWSEWPGVPVNNLFYAEFNNRGPGADVSKRVHWT 300

Query: 301 TFHILDKQFAIQFTVDNFINASEWLPETGVPFRRGL 336
            F +LDKQ A QFTVDNF+N S+WLPETG+PFR GL
Sbjct: 301 GFRVLDKQSAYQFTVDNFVNGSKWLPETGIPFRDGL 334

[Close]

BLAST of Lsi02G004480 vs. NCBI nr
Match: KAA0055287.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >TYJ99210.1 putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 531.6 bits (1368), Expect = 5.1e-147
Identity = 258/335 (77.01%), Postives = 288/335 (85.97%), Query Frame = 0


Query: 1   MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGS-KDFKSISEAIATAPDNSNIRFYI 60
           MEI K V I + CFFNL+   +HS  F+  VSLDGS  DFK+I+ AIA APDNSN RFYI
Sbjct: 1   MEILKAVVITVMCFFNLVPF-IHSFTFNATVSLDGSMNDFKTIAAAIAAAPDNSNTRFYI 60

Query: 61  HVTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQ 120
            VTPGTYHERLQIP TKTFIAL+GDNALTTIIVD+RSN  GFKT+DSATLTV+GDNF+A+
Sbjct: 61  RVTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGDNFIAK 120

Query: 121 SLTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFI 180
           SLTFENSAGPQNGQAVAVLDQA  TAYHKCRFLGFQDTLYV G+  FFKECD+YG+VDFI
Sbjct: 121 SLTFENSAGPQNGQAVAVLDQARFTAYHKCRFLGFQDTLYVGGKSHFFKECDVYGTVDFI 180

Query: 181 FGHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQI 240
           FG+GL MFQDCNIYAR PN+  TVTAQSKE+  + SGFSFQNCTVTVSSEI A+KA+V+I
Sbjct: 181 FGNGLTMFQDCNIYAREPNRPITVTAQSKEQLHEESGFSFQNCTVTVSSEIVASKANVKI 240

Query: 241 YLGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWT 300
           YL RPWRQYS+VVFMESFLD+ V PEGWSEW GV  NNLFY EFNNRGPGADVSKRV+W 
Sbjct: 241 YLDRPWRQYSRVVFMESFLDEEVLPEGWSEWPGVPTNNLFYGEFNNRGPGADVSKRVHWI 300

Query: 301 TFHILDKQFAIQFTVDNFINASEWLPETGVPFRRG 335
            FH+L+KQ A QFT+DNF+N S WLPETG+PFRRG
Sbjct: 301 GFHLLNKQLANQFTIDNFVNGSYWLPETGIPFRRG 334

[Close]

BLAST of Lsi02G004480 vs. NCBI nr
Match: XP_004152582.2 (pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [Cucumis sativus])

HSP 1 Score: 520.0 bits (1338), Expect = 1.5e-143
Identity = 250/335 (74.63%), Postives = 288/335 (85.97%), Query Frame = 0


Query: 1   MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIH 60
           M  F  V   LFCFFNLM++ +HS++F++ VSLDGS +FK IS+AIA AP+NSN RFYIH
Sbjct: 1   MLFFFGVPCSLFCFFNLMLL-IHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIH 60

Query: 61  VTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQS 120
           V PGTYHE LQIP+ K FIALVGD+  TTII+DNRSN  GFKT +SATLTV GDNF+A+S
Sbjct: 61  VAPGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAES 120

Query: 121 LTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIF 180
           LTFENSAGPQN QAVAV D+A+HTAY+KCRFL FQDTLYV G+PQFFKE DIYGSVDFI 
Sbjct: 121 LTFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFIC 180

Query: 181 GHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIY 240
           G+G VMFQDCNIYAR+P  S TVTAQSK   R VSGFSFQNCTVTVS EI++NK +V+++
Sbjct: 181 GYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVF 240

Query: 241 LGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWTT 300
           LGRPW+QYSKVVFMESFLDDVVA EGW EW GV +NNLFY EFNN GPGADVSKRVNWT+
Sbjct: 241 LGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTS 300

Query: 301 FHILDKQFAIQFTVDNFINASEWLPETGVPFRRGL 336
           +H+LDK+ A++FTVDNF+N SEWLPETG+PFRRGL
Sbjct: 301 YHLLDKESALRFTVDNFVNGSEWLPETGIPFRRGL 334

[Close]

BLAST of Lsi02G004480 vs. NCBI nr
Match: KAA0055286.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 516.9 bits (1330), Expect = 1.3e-142
Identity = 244/318 (76.73%), Postives = 278/318 (87.42%), Query Frame = 0


Query: 19  MISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTKTF 78
           M+ VHSI+F++ VSLDGS +FK IS+AI  AP+NSN RFYIHV PGTYHE +QIPK K F
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVL 138
           IAL+GD+A TTII+DNRSN  GFKT +SATLTV GDNF+A+SLTFENSAGPQNGQAVAV 
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 DQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIPN 198
           D+ADHTAY+KCRFLGFQDTLYV G+ QFFKE +IYGSVDFIFGHG VMFQDCNIYAR+P+
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 199 KSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFL 258
            S TVTAQSK+  R +SGFSFQNCTVTVS EI++NK +V+++LGRPWRQYS VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 259 DDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFI 318
           DDVVA EGW EW GV +NNLFY EFNN GPGADVSKRVNWT +H+LDK+ AI FTVDNFI
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 319 NASEWLPETGVPFRRGLL 337
           + SEWL ETG+PFRRGLL
Sbjct: 301 DGSEWLAETGIPFRRGLL 318

[Close]

BLAST of Lsi02G004480 vs. NCBI nr
Match: KAE8648077.1 (hypothetical protein Csa_018832 [Cucumis sativus])

HSP 1 Score: 513.8 bits (1322), Expect = 1.1e-141
Identity = 252/336 (75.00%), Postives = 290/336 (86.31%), Query Frame = 0


Query: 1   MEIFKVVAILLFCFFNLMMISVHSINFDVMVSLDGSKD-FKSISEAIATAPDNSNIRFYI 60
           M+  KV  I+L  FFNL++  + S  F+  VSLDGS D FK+I++AIA+ P+N N RFYI
Sbjct: 1   MKNLKVETIILLFFFNLLLY-IQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYI 60

Query: 61  HVTPGTYHERLQIPKTKTFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQ 120
           HVTPGTYHE LQIP TKTFIAL+GDNALTTIIVD+RSN  GFKT DSATLTV+G+NFLAQ
Sbjct: 61  HVTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQ 120

Query: 121 SLTFENSAGPQNGQAVAVLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFI 180
           SLTF+NSAGPQNGQAVAVLD+A  T Y+KCRFLGFQDTLYV+G+PQFFKECDIYGSVDFI
Sbjct: 121 SLTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFI 180

Query: 181 FGHGLVMFQDCNIYARIPNKSNTVTAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQI 240
           FG GLVMFQDCNIYAR PN+S T+TAQSK++  + SGFSFQNCT+T+SSEIAANKA V+I
Sbjct: 181 FGDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKI 240

Query: 241 YLGRPWRQYSKVVFMESFLDDVVAPEGWSEWSGVSINNLFYAEFNNRGPGADVSKRVNWT 300
           YLGRPWRQYS+VVFMESFLD  V P+GW +WSGV +NNLFY EFNNRGPGADVSKR++ T
Sbjct: 241 YLGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCT 300

Query: 301 TFHILDKQFAIQFTVDNFINASEWLPETGVPFRRGL 336
            FH+LDKQ A QFT+ NF+N S+WLPETGVPFR GL
Sbjct: 301 GFHVLDKQSANQFTI-NFVNGSDWLPETGVPFRSGL 334

[Close]

BLAST of Lsi02G004480 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 291.6 bits (745), Expect = 8.2e-79
Identity = 132/314 (42.04%), Postives = 207/314 (65.92%), Query Frame = 0


Query: 29  VMVSLDGSKDFKSISEAIATAPDNSNIR---FYIHVTPGTYHERLQIPKTKTFIALVGDN 88
           V VS DG+ +F +I+ A+A AP+N++     F I+VT G Y E + I K K ++ ++GD 
Sbjct: 265 VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 324

Query: 89  ALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTA 148
              T++  NRS   G+ T +SAT  V   NF+A ++TF N+AGP+  QAVA+   AD + 
Sbjct: 325 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 384

Query: 149 YHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYAR--IPNKSNTV 208
           ++ C F  +QDTLY     QF++ECD+YG+VDFIFG+  V+FQ+CN+Y R  +PN+ N +
Sbjct: 385 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 444

Query: 209 TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVA 268
           TAQ +      +G S QNCT+  + ++ ++  +V+ YLGRPW++YS+ V+M+S++D  V 
Sbjct: 445 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 504

Query: 269 PEGWSEWSG-VSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASE 328
           P GW EW+G  +++ L+YAE+NN GPG++ + RV W  +H+++   A  FTV      ++
Sbjct: 505 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEAD 564

Query: 329 WLPETGVPFRRGLL 337
           W+ +TGVP+  GL+
Sbjct: 565 WIWKTGVPYTSGLI 578

[Close]

BLAST of Lsi02G004480 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 288.5 bits (737), Expect = 6.9e-78
Identity = 136/322 (42.24%), Postives = 208/322 (64.60%), Query Frame = 0


Query: 17  LMMISVHSINFDVMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTK 76
           L+   V   N+++ V++DG+ +F +I++A+  AP+ S  RF I++  G Y E +++PK K
Sbjct: 210 LLEAPVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKK 269

Query: 77  TFIALVGDNALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVA 136
           T I  +GD    T+I  NRS   G+ T  + T+ V G  ++A+ ++F NSAGP   QAVA
Sbjct: 270 TMIMFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVA 329

Query: 137 VLDQADHTAYHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARI 196
               +DH+A+++C F G+QDTLYV    QF++ECDIYG++DFIFG+  V+FQ+ ++YAR 
Sbjct: 330 FRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARK 389

Query: 197 PNKSNTV--TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFM 256
           PN  + +  TAQS+ +S   +G S  NC +  + ++   K + + YLGRPWR+YS+ V +
Sbjct: 390 PNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVII 449

Query: 257 ESFLDDVVAPEGWSEW-SGVSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQF-AIQF 316
           +SF+DD++ P GW E     ++  L+Y E+ N GPGA+++KRV W  F  ++ Q  A QF
Sbjct: 450 KSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQF 509

Query: 317 TVDNFINASEWLPETGVPFRRG 335
           TV  FI+ S WL  TG+PF  G
Sbjct: 510 TVGPFIDGSTWLNSTGIPFSLG 531

[Close]

BLAST of Lsi02G004480 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 288.5 bits (737), Expect = 6.9e-78
Identity = 133/314 (42.36%), Postives = 206/314 (65.61%), Query Frame = 0


Query: 29  VMVSLDGSKDFKSISEAIATAP---DNSNIRFYIHVTPGTYHERLQIPKTKTFIALVGDN 88
           V V+ +G+ +F +I+EA+ +AP   D +   F I+VT G Y E + I K K ++ ++GD 
Sbjct: 259 VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 318

Query: 89  ALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTA 148
              T++  NR+   G+ T +SAT  V   NF+A ++TF N+AGP+  QAVA+   AD + 
Sbjct: 319 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 378

Query: 149 YHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIP--NKSNTV 208
           ++ C F  +QDTLY     QF++ECDIYG+VDFIFG+  V+FQDCN+Y R P  N+ N +
Sbjct: 379 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 438

Query: 209 TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVA 268
           TAQ +      +G S  NCT+  + ++ ++  +V+ YLGRPW++YS+ VFM+S++D+VV 
Sbjct: 439 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 498

Query: 269 PEGWSEWSG-VSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASE 328
           P GW EW+G  +++ L+YAE+NN G G+  + RV W  +H+++   A  FTV+NF+    
Sbjct: 499 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 558

Query: 329 WLPETGVPFRRGLL 337
           W+ ++GVP+  GLL
Sbjct: 559 WMVQSGVPYISGLL 572

[Close]

BLAST of Lsi02G004480 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 287.7 bits (735), Expect = 1.2e-77
Identity = 133/314 (42.36%), Postives = 201/314 (64.01%), Query Frame = 0


Query: 29  VMVSLDGSKDFKSISEAIATAP---DNSNIRFYIHVTPGTYHERLQIPKTKTFIALVGDN 88
           V V  +G+ +F +I+ AIA AP   D SN  F I+VT G Y E +++PK K ++ ++GD 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 89  ALTTIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTA 148
              T+I  NRS   G+ T +SAT  + G NF+  ++T  N+AGP  GQAVA+    D + 
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 149 YHKCRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARIPNK--SNTV 208
           ++ C F  +QDTLY     QF++ECD+YG+VDFIFG+  V+ Q+CN+Y R P K  SN V
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEV 425

Query: 209 TAQSKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVA 268
           TAQ +      +G +   CT+  + ++A +  +V+ YLGRPW++YS+ V M++++D  + 
Sbjct: 426 TAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLE 485

Query: 269 PEGWSEWSG-VSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASE 328
           P GW+ WSG  +++ L+YAE+NN GPG+D + RV W  +H+++   A  FTV NF+    
Sbjct: 486 PSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEG 545

Query: 329 WLPETGVPFRRGLL 337
           W+ +TGVPF  GL+
Sbjct: 546 WIGQTGVPFVGGLI 559

[Close]

BLAST of Lsi02G004480 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 285.8 bits (730), Expect = 4.5e-77
Identity = 134/310 (43.23%), Postives = 199/310 (64.19%), Query Frame = 0


Query: 29  VMVSLDGSKDFKSISEAIATAPDNSNIRFYIHVTPGTYHERLQIPKTKTFIALVGDNALT 88
           ++V+ DG+ +F +I+EAI+ AP+ SN R  I+V  G Y E + IP  KT I L+GD +  
Sbjct: 238 LVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDV 297

Query: 89  TIIVDNRSNRHGFKTTDSATLTVDGDNFLAQSLTFENSAGPQNGQAVAVLDQADHTAYHK 148
           T I  NRS   G+ T  SATL V G+ FLA+ +   N+AGP+  QAVA+   AD  A ++
Sbjct: 298 TFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYR 357

Query: 149 CRFLGFQDTLYVKGRPQFFKECDIYGSVDFIFGHGLVMFQDCNIYARI--PNKSNTVTAQ 208
           C   G+QDTLY     QF++ECDIYG++D+IFG+  V+FQ CNI +++  P +   +TAQ
Sbjct: 358 CVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQ 417

Query: 209 SKERSRDVSGFSFQNCTVTVSSEIAANKASVQIYLGRPWRQYSKVVFMESFLDDVVAPEG 268
           S++   + +G S QNC++  S ++  +   V+ YLGRPWR++S+ V MES++D+ +   G
Sbjct: 418 SRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSG 477

Query: 269 WSEWS-GVSINNLFYAEFNNRGPGADVSKRVNWTTFHILDKQFAIQFTVDNFINASEWLP 328
           WS+W+ G +++ L+Y E+NN GPG++  KRVNW  FHI+  + A  FT   FI    WL 
Sbjct: 478 WSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWLG 537

Query: 329 ETGVPFRRGL 336
            T  P+  G+
Sbjct: 538 STSFPYDNGI 547

The following BLAST results are available for this feature:

BLAST of Lsi02G004480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	1.8e-78	45.66	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9SRX4	1.2e-77	42.04	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
O81415	9.8e-77	42.24	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
Q8RXK7	9.8e-77	42.36	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
O22256	1.7e-76	42.36	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Lsi02G004480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BKF8	2.4e-150	79.46	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00421...	[more]
A0A5D3BHF7	2.4e-147	77.01	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00420...	[more]
A0A0A0LR14	7.4e-144	74.63	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1	[more]
A0A5A7ULX7	6.2e-143	76.73	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290...	[more]
A0A5D3BM28	2.1e-138	75.16	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00418...	[more]

BLAST of Lsi02G004480 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0055288.1	4.9e-150	79.46	putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >...	[more]
KAA0055287.1	5.1e-147	77.01	putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >...	[more]
XP_004152582.2	1.5e-143	74.63	pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [...	[more]
KAA0055286.1	1.3e-142	76.73	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]
KAE8648077.1	1.1e-141	75.00	hypothetical protein Csa_018832 [Cucumis sativus]	[more]

BLAST of Lsi02G004480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02810.1	8.2e-79	42.04	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	6.9e-78	42.24	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	6.9e-78	42.36	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	1.2e-77	42.36	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26440.1	4.5e-77	43.23	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 22..335 e-value: 6.1E-113 score: 379.4
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 28..320 e-value: 1.7E-106 score: 355.3
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 19..333
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 19..333
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 172..181
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 26..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G004480.1	Lsi02G004480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity