Lsi02G004390 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G004390
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cytochrome P450 71A1-like
Location	chr02: 3647154 .. 3650615 (+)
RNA-Seq Expression	Lsi02G004390
Synteny	Lsi02G004390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATGAAAGGGGATGTTACATATCATTTGAGATTCTTTCACTTTTGATATTAAATAATTTAATAATTCAATATAGTTAAGTGATAATTAATTTCTCGTTATAACATTATATGGAGAAGTGCATAATATATTAGATAATATACTAAATATTATCTAATATATTATAAATTATATGAAACAAAATAATAATAATACAATTTATTTAATATAAAATATGTTCATAAGTTAATTAATACTAATTTATATGTCACTTAATATTAAGTGAAGCTAGAATACAGATCTACCCGTGATTAAGAGATCTAAAATTCATGCTTAATATACTAAAAAAAATATATTGAGTGGATAACTATAAATCTACTATTTATAAATGTATTTCTTATCTATTTTTTAATGATTTATAAATACATTATCTTTCGTTGACTCCTTGCTTTCCAAACAAAAAATAATTAAAAAAAAATATAATAAAAGAATATGCAGAATGCTGCACGAGGCACACAGCTGGCCTTGGCAGGTGAGGAGACCCGCCAAGTGCAGGGACAATCTGGGGTAGTGGTTTTCTTTTAGTTATTATATTATATAATACTATTATTAAAAAAAAAAAATAGTTAGATACATTTTGGGTGCATCTATTTTTATATATATAAAAAGAAAGCATAGTAGATAAAGACAATGAAAAAAAAATAGAAAATATAACACTTCTTTAAATAGTTATAAATATAGTAAATATTGACTAGTCAACTATTAAATTTTTTTTTTTTTTTTTTTGGTAAAAATTTCAAATTTGCCATATGTATATCAGGACACCATCTTCTTCTTTCTTTTCTAATTTTTTTGTCCTTTTTTCGATTGGTGCAGGTGTAGGTGCGGAAGCGGGAGCTGGAGCTGTGTGCGGGTGGGGACGTCAGTGGTGTAGGTGGAGGAGTTGTCGGTGGTGGGAAGCAAGAGGTGGAGTTTCCAAAAGTGAGATTTAAAATTGGATGTCAGTTCGAACAAGAAAGCCGCAGCGGCGTCGGTGAACACGAGGTGGGGCGGCCTTCAGATGGAAGAGACAAGAGAGAAGGCTCGAGCTGTTGAGGACGATGGCAACGGTGTGAGCATGGGCAGAGGGTGGCGACGAATGGCGTGTGCATGACAGAGAAAATGAGTTTTAAAGTTTAAATGGGAGAGACTCAAATAACAAAGTATTTGATTGCAAGAGGAAAGGGATGACTAGAGGATATTATTTTCCTGATATTTCTCGAAAATTATCATGGATAGAAGCTATCCGTGGTATTCACTGATATCAACTATTAGTGATATTAATGATAGCAACTAAGAAGCACATGAAAGGGGTCATTGAGTGTGGGCTTTTTAATTTTTTTTTATCAATTTTTATGTTATATATACAATTATTTTATTCTTGTGCTATATATATATTCTATTATTTTGAACTTAAATGATATGCAACTATTCCAAAAAAAATTAGTATAGAATATGAAAAATTTTCTACACTCCTATTTATTTGGTGAAATGTTAGCATGTTTATAATAATAAGGTGAGATTTATTGGTTGACTAATATTTGTGAAGAAAAATTGATAGAGTGAATGATATTTATGTAGGACTAAAAAATTTCTCAAAATACAAGGCTGCAGTCATCTTGTTGGCGTCACCAAATTGGTGACTATGGAATCAGTAGCTGAAGATTATAAATGATGTAGAGGATTAAAAACAAACCATTTGTAAACAAAAAAAAAAAAAAAAAAATGCCAATCCTTTCACTGGCCTCATTCTCCACATCAATCTTCTTCATTTCGTTTTTGATTCTCCTTCTAATCCCCAAACTCAAATCAAAACCCAACAAGAAACCCATCAATCTCCCACCATCTCCACCAAAGCTACCCTTAATTGGCAACCTTCACCAAGTAGGCGACTATCCCCATCTCTGTTTCCGCCGCCTCTCCCACAAATTCGGCCCCATCATCCTTTTACAACTCGGACAAATCCCAACTCTCATAATCTCATCTTCAAAAATCGCCAAATAAGCTTTCAAAACCCACGATCTCTCCTTCTCAAGCCGCCCCTTTCTTTACTCCGCTCAACACATCTTCTACAACTGCACCGACGTTGTTTTTTCCCCTTACGGTTCTTATTGGCGCCAAATTAGAAAAATCTGCATCCTCGAACTCCTTAGCGCCAAAAGGGTTCAATCTTTTGCTCTCCTTCGACAACAAGAAGTTGCCCGTTTGGTTGATAGAATTTCACAATCACAGTCACATAATCCCCTGGATTTGTCCACTTTCTTGGCGTTGTATGCCAATGATGTGGTATGTCGAACCGCGCTTGGGAGGGGATTTTCCGCCGGTGGTGAGTACCATCTTCATGGGATTAAGGAGCTACTTGAAGAACTTCAGATTTTGCTTGGTGGGTTCTGTATTGGGGATTTGTTCCCTTCTTTGGCGTTTCTCAGTAAGTTCACAGGGATGAAATCTAGGCTTGTGAGGACAGTTAAAGGGTTCGATAAGTTGTTTGACCAAGTTATAGCCGAACATCTCAATCCCGAGAGAGAAATATTGGAGGAATCGTCTGAGGATCTTGTTGATGTTTTGCTTGATATACAGAAGGATGGATCTGATGATAAAATTCCACTTACTATGGATAATGTTAAGGCCATCATTTTGGTATAACACCGTCTGTTTTCCTCTGTTTTACTCTGTTATTATCGAAAGTGACTGACCTATGTGGGCAATTTCTGTAGGATATGTTTGCGGCAGGGACTGACACAACATTTATAACTCTTGATTGGGGGATGACAGAGCTGATAAGACACCCAAGGGCCATGGAAAGAGCACAAAACGAAATTCGAAGTATTGTTGGTGAGAGAAGAATTGTATCAGAGAGTGATCTTTTAGAAATGCCTTATTTGAAAGCTGTGGTTAAAGAGGTGCTTCGATTACATCCACCGGCGCCGGTTTCAGTTGCGAGAGAAACTACGGAAGATGTGAGCATTGAAGGCTATGACATACCAGCTAAAACGAGGGTTTTTGTGAATGTTTGGGCAATTGGGAGAGACCCAGAATCGTGGAAGGATCCAGAAAGTTTTGAGCCAGAGAGATTTCTGGAGAGTGAAGTTGACTACAGAGGGTTGGATTTTGAGTTCATTCCATTCGGGGCTGGAAGAAGGATTTGTCCAGGTATTAAATTGGGGATCGTCATGATAGAGCTTGCTTTGGCTCAGATTTTGCATAGCTTTGATTGGGAGCTTCCCAATGGAATTGAAGCTACGGATTTGGATATGACTGAAGTTTATGGAGTTACAATGCATAGAAAAGCTCACTTGGACGTTGTGGCAAAACCTTATTTTTTCTAATCCACATCTCAAATTAATTAGGTTTGTACTTAAAAAAGATGCAAAATTTAAAGAAAATAAATTTAATTTTTAAAAATAAAAAACGAAATGGTTACCAAACCAGACTTAACTA

mRNA sequence

ATGGAATGCTGCACGAGGCACACAGCTGGCCTTGGCAGTTCGAACAAGAAAGCCGCAGCGGCGTCGGTGAACACGAGGTGGGGCGGCCTTCAGATGGAAGAGACAAGAGAGAAGGCTCGAGCTGTTGAGGACGATGGCAACGAAACCCATCAATCTCCCACCATCTCCACCAAAGCTACCCTTAATTGGCAACCTTCACCAAGTAGGCGACTATCCCCATCTCTGTTTCCGCCGCCTCTCCCACAAATTCGGCCCCATCATCCTTTTACAACTCGGACAAATCCCAACTCTCATAATCTCATCTTCAAAAATCGCCAAATAAGCTTTCAAAACCCACGATCTCTCCTTCTCAAGCCGCCCCTTTCTTTACTCCGCTCAACACATCTTCTACAACTGCACCGACGTTGTTTTTTCCCCTTACGAAAAATCTGCATCCTCGAACTCCTTAGCGCCAAAAGGGTTCAATCTTTTGCTCTCCTTCGACAACAAGAAGTTGCCCGTTTGGTTGATAGAATTTCACAATCACAGTCACATAATCCCCTGGATTTGTCCACTTTCTTGGCGTTGTATGCCAATGATGTGGTATGTCGAACCGCGCTTGGGAGGGGATTTTCCGCCGGTGGTGAGTACCATCTTCATGGGATTAAGGAGCTACTTGAAGAACTTCAGATTTTGCTTGGTGGGTTCTGTATTGGGGATTTGTTCCCTTCTTTGGCGTTTCTCAGTAAGTTCACAGGGATGAAATCTAGGCTTGTGAGGACAGTTAAAGGGTTCGATAAGTTGTTTGACCAAGTTATAGCCGAACATCTCAATCCCGAGAGAGAAATATTGGAGGAATCGTCTGAGGATCTTGTTGATGTTTTGCTTGATATACAGAAGGATGGATCTGATGATAAAATTCCACTTACTATGGATAATGTTAAGGCCATCATTTTGGATATGTTTGCGGCAGGGACTGACACAACATTTATAACTCTTGATTGGGGGATGACAGAGCTGATAAGACACCCAAGGGCCATGGAAAGAGCACAAAACGAAATTCGAAGTATTGTTGGTGAGAGAAGAATTGTATCAGAGAGTGATCTTTTAGAAATGCCTTATTTGAAAGCTGTGGTTAAAGAGGTGCTTCGATTACATCCACCGGCGCCGGTTTCAGTTGCGAGAGAAACTACGGAAGATGTGAGCATTGAAGGCTATGACATACCAGCTAAAACGAGGGTTTTTGTGAATGTTTGGGCAATTGGGAGAGACCCAGAATCGTGGAAGGATCCAGAAAGTTTTGAGCCAGAGAGATTTCTGGAGAGTGAAGTTGACTACAGAGGGTTGGATTTTGAGTTCATTCCATTCGGGGCTGGAAGAAGGATTTGTCCAGGTATTAAATTGGGGATCGTCATGATAGAGCTTGCTTTGGCTCAGATTTTGCATAGCTTTGATTGGGAGCTTCCCAATGGAATTGAAGCTACGGATTTGGATATGACTGAAGTTTATGGAGTTACAATGCATAGAAAAGCTCACTTGGACGTTGTGGCAAAACCTTATTTTTTCTAATCCACATCTCAAATTAATTAGGTTTGTACTTAAAAAAGATGCAAAATTTAAAGAAAATAAATTTAATTTTTAAAAATAAAAAACGAAATGGTTACCAAACCAGACTTAACTA

Coding sequence (CDS)

ATGGAATGCTGCACGAGGCACACAGCTGGCCTTGGCAGTTCGAACAAGAAAGCCGCAGCGGCGTCGGTGAACACGAGGTGGGGCGGCCTTCAGATGGAAGAGACAAGAGAGAAGGCTCGAGCTGTTGAGGACGATGGCAACGAAACCCATCAATCTCCCACCATCTCCACCAAAGCTACCCTTAATTGGCAACCTTCACCAAGTAGGCGACTATCCCCATCTCTGTTTCCGCCGCCTCTCCCACAAATTCGGCCCCATCATCCTTTTACAACTCGGACAAATCCCAACTCTCATAATCTCATCTTCAAAAATCGCCAAATAAGCTTTCAAAACCCACGATCTCTCCTTCTCAAGCCGCCCCTTTCTTTACTCCGCTCAACACATCTTCTACAACTGCACCGACGTTGTTTTTTCCCCTTACGAAAAATCTGCATCCTCGAACTCCTTAGCGCCAAAAGGGTTCAATCTTTTGCTCTCCTTCGACAACAAGAAGTTGCCCGTTTGGTTGATAGAATTTCACAATCACAGTCACATAATCCCCTGGATTTGTCCACTTTCTTGGCGTTGTATGCCAATGATGTGGTATGTCGAACCGCGCTTGGGAGGGGATTTTCCGCCGGTGGTGAGTACCATCTTCATGGGATTAAGGAGCTACTTGAAGAACTTCAGATTTTGCTTGGTGGGTTCTGTATTGGGGATTTGTTCCCTTCTTTGGCGTTTCTCAGTAAGTTCACAGGGATGAAATCTAGGCTTGTGAGGACAGTTAAAGGGTTCGATAAGTTGTTTGACCAAGTTATAGCCGAACATCTCAATCCCGAGAGAGAAATATTGGAGGAATCGTCTGAGGATCTTGTTGATGTTTTGCTTGATATACAGAAGGATGGATCTGATGATAAAATTCCACTTACTATGGATAATGTTAAGGCCATCATTTTGGATATGTTTGCGGCAGGGACTGACACAACATTTATAACTCTTGATTGGGGGATGACAGAGCTGATAAGACACCCAAGGGCCATGGAAAGAGCACAAAACGAAATTCGAAGTATTGTTGGTGAGAGAAGAATTGTATCAGAGAGTGATCTTTTAGAAATGCCTTATTTGAAAGCTGTGGTTAAAGAGGTGCTTCGATTACATCCACCGGCGCCGGTTTCAGTTGCGAGAGAAACTACGGAAGATGTGAGCATTGAAGGCTATGACATACCAGCTAAAACGAGGGTTTTTGTGAATGTTTGGGCAATTGGGAGAGACCCAGAATCGTGGAAGGATCCAGAAAGTTTTGAGCCAGAGAGATTTCTGGAGAGTGAAGTTGACTACAGAGGGTTGGATTTTGAGTTCATTCCATTCGGGGCTGGAAGAAGGATTTGTCCAGGTATTAAATTGGGGATCGTCATGATAGAGCTTGCTTTGGCTCAGATTTTGCATAGCTTTGATTGGGAGCTTCCCAATGGAATTGAAGCTACGGATTTGGATATGACTGAAGTTTATGGAGTTACAATGCATAGAAAAGCTCACTTGGACGTTGTGGCAAAACCTTATTTTTTCTAA

Protein sequence

MECCTRHTAGLGSSNKKAAAASVNTRWGGLQMEETREKARAVEDDGNETHQSPTISTKATLNWQPSPSRRLSPSLFPPPLPQIRPHHPFTTRTNPNSHNLIFKNRQISFQNPRSLLLKPPLSLLRSTHLLQLHRRCFFPLRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGVTMHRKAHLDVVAKPYFF

Homology

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BLAST of Lsi02G004390 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-139
Identity = 246/375 (65.60%), Postives = 303/375 (80.80%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTA 199
           +RKICILELLSAKRVQSF+ +R++EVARLV R+++       +L+  L LYANDV+CR A
Sbjct: 143 IRKICILELLSAKRVQSFSHVREEEVARLVRRVAEFYP-GTTNLTKMLGLYANDVLCRVA 202

Query: 200 LGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFD 259
            GRGFS GG+Y  HG +++LEE Q LLGGF IGD FPS+ F+   TGMKSRL  T + FD
Sbjct: 203 FGRGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETFRRFD 262

Query: 260 KLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGT 319
           +LFDQ++ +HL+P+RE  +E  +DLVDVLLDIQK  S + +PLTMDNVKAIILDMFAAGT
Sbjct: 263 ELFDQMVTDHLSPKRE--KEEHKDLVDVLLDIQKKESTE-MPLTMDNVKAIILDMFAAGT 322

Query: 320 DTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLH 379
           DTTFITLDWGMTEL+ + + +ERAQ E+R +VGERR+V ESDL ++ Y+KAV+KE+ RLH
Sbjct: 323 DTTFITLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFRLH 382

Query: 380 PPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEVDY 439
           PPAPV V RE+ EDV+I+GYDI AKTR+FVN WAIGRDPESW+DPE+FEPERF+ S +D+
Sbjct: 383 PPAPVLVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTIDF 442

Query: 440 RGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGVT 499
           +G DFE IPFGAGRR CP +  G   IELALAQ+LH+FDWELP    A DLDMTEV+G+T
Sbjct: 443 KGQDFELIPFGAGRRGCPAVTFGTATIELALAQLLHTFDWELPLDTAAKDLDMTEVFGIT 502

Query: 500 MHRKAHLDVVAKPYF 515
           MHR A+L VVA+P F
Sbjct: 503 MHRIANLIVVARPRF 513

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BLAST of Lsi02G004390 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 362.8 bits (930), Expect = 6.2e-99
Identity = 187/375 (49.87%), Postives = 254/375 (67.73%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQS-QSHNPLDLSTFLALYANDVVCRT 199
           +RKIC+LELLS KRV S+  +R++EV  +++RISQS  +   ++LS  L L ++  + R 
Sbjct: 126 VRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRV 185

Query: 200 ALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGF 259
           A G+ +  G E   +   +L  EL  L+G F +GD FPS A++   TGM +RL R     
Sbjct: 186 AFGKKYE-GEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGEL 245

Query: 260 DKLFDQVIAEHLNPEREILEESSE--DLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFA 319
           D   D VI +HL   +    +  E  DLVDVLL +QKD S   + L  +N+KA+ILDMF+
Sbjct: 246 DAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLG-VHLNRNNLKAVILDMFS 305

Query: 320 AGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVL 379
            GTDTT +TL+W M ELI+HP  ME+AQ E+R +VG++  V E DL ++ YLK ++KE L
Sbjct: 306 GGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETL 365

Query: 380 RLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESE 439
           RLHP AP+ V RE+T DV I GY IPAKTRVF+N WAIGRDP+SW++ E F PERF+ + 
Sbjct: 366 RLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNS 425

Query: 440 VDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVY 499
           VD++G DF+ IPFGAGRR CPGI  GI  +E++LA +L+ F+WELP  +   DLDM+E  
Sbjct: 426 VDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAV 485

Query: 500 GVTMHRKAHLDVVAK 512
           G+T+H K  L +VAK
Sbjct: 486 GITVHMKFPLQLVAK 498

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BLAST of Lsi02G004390 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.4e-98
Identity = 188/377 (49.87%), Postives = 249/377 (66.05%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTA 199
           +R IC+L LLS +RV+SF  +R++E   ++  I  S S + L+LS       NDVVC+ A
Sbjct: 144 VRSICVLNLLSNRRVRSFRSVREEETKSMIRNIKGSSS-SVLNLSEMFVRLTNDVVCKVA 203

Query: 200 LGRGFSAG-GEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGF 259
           LGR +S G G       KE+L E   LLG   IGD  P L++LS   G+ ++L +  K  
Sbjct: 204 LGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAKLDKVAKQL 263

Query: 260 DKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAG 319
           D   D V+ EH+N      ++  +D +D+LL IQK+ S   IP+   +VK IILDMFAAG
Sbjct: 264 DDFIDTVVQEHMNHSSRSGDDDQKDFLDILLAIQKETSAG-IPIDGVSVKGIILDMFAAG 323

Query: 320 TDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERR-IVSESDLLEMPYLKAVVKEVLR 379
           TDTT+  L+W MTEL+RHPR M + QNE+R IVG R  +++E DL+EM YLKAV KE LR
Sbjct: 324 TDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNRTDVITEDDLVEMHYLKAVTKETLR 383

Query: 380 LHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEV 439
           LHPP P+ V R +T DV + GY+I A T+VF++ W IGRDP+ +  PE FEPERFL + +
Sbjct: 384 LHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKPEEFEPERFLNNGI 443

Query: 440 DYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYG 499
           DY+G DFE IPFGAGRR+CPGI+  + + E+ALA I+H FDW LP+     DLDMTE  G
Sbjct: 444 DYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANIVHKFDWALPDEASGEDLDMTETTG 503

Query: 500 VTMHRKAHLDVVAKPYF 515
           +T H+K  L  VA P+F
Sbjct: 504 LTAHKKYPLKAVAFPHF 518

[Close]

BLAST of Lsi02G004390 vs. ExPASy Swiss-Prot
Match: O81970 (Cytochrome P450 71A9 OS=Glycine max OX=3847 GN=CYP71A9 PE=2 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.9e-96
Identity = 195/416 (46.88%), Postives = 271/416 (65.14%), Query Frame = 0


Query: 101 IFKNRQISFQNPRSLLLKPPLSLLRSTHLLQLHRRCFFPLRKICILELLSAKRVQSFALL 160
           IFKN    F    SL     L    ST     +   +  +RKI ILELLS KRVQSF  +
Sbjct: 89  IFKNHDSVFSGRPSLYAANRLG-YGSTVSFAPYGEYWREMRKIMILELLSPKRVQSFEAV 148

Query: 161 RQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTALGRGFSAGGEYHLHGIKELLE 220
           R +EV  L+  I  + SH P++LS       N++VCR ALG+   +G +   + + E+L+
Sbjct: 149 RFEEVKLLLQTI--ALSHGPVNLSELTLSLTNNIVCRIALGKRNRSGAD-DANKVSEMLK 208

Query: 221 ELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFDKLFDQVIAEHL---NPEREIL 280
           E Q +LGGF   D FP L +L+KF+G+++RL +  +  D  +DQVI EH+   + ER   
Sbjct: 209 ETQAMLGGFFPVDFFPRLGWLNKFSGLENRLEKIFREMDNFYDQVIKEHIADNSSERSGA 268

Query: 281 EESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGTDTTFITLDWGMTELIRHP 340
           E   ED+VDVLL +QKD  +  I +T D +K +++D+F AGTDT   T+ W M+ELIR+P
Sbjct: 269 EH--EDVVDVLLRVQKD-PNQAIAITDDQIKGVLVDIFVAGTDTASATIIWIMSELIRNP 328

Query: 341 RAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLHPPAPVSVARETTEDVSIE 400
           +AM+RAQ E+R +V  + +V E DL ++ Y+K+VVKEVLRLHPPAP+ V RE TE+ +I+
Sbjct: 329 KAMKRAQEEVRDLVTGKEMVEEIDLSKLLYIKSVVKEVLRLHPPAPLLVPREITENCTIK 388

Query: 401 GYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEVDYRGLDFEFIPFGAGRRICP 460
           G++IPAKTRV VN  +I  DP  W++P  F PERFL S +D++G  FE +PFG GRR CP
Sbjct: 389 GFEIPAKTRVLVNAKSIAMDPCCWENPNEFLPERFLVSPIDFKGQHFEMLPFGVGRRGCP 448

Query: 461 GIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGVTMHRKAHLDVVAKPY 514
           G+   + ++ELALA +L  FDWELP G+   DLDM E  G+T+H+KAHL + A P+
Sbjct: 449 GVNFAMPVVELALANLLFRFDWELPLGLGIQDLDMEEAIGITIHKKAHLWLKATPF 497

[Close]

BLAST of Lsi02G004390 vs. ExPASy Swiss-Prot
Match: O04164 (Cytochrome P450 71A6 (Fragment) OS=Nepeta racemosa OX=54731 GN=CYP71A6 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 6.7e-93
Identity = 185/440 (42.05%), Postives = 270/440 (61.36%), Query Frame = 0


Query: 88  PFTTRTNPNSHNLIFKNRQISFQNPRSLLLKPPLSLLRSTHLLQL--HRRCFFPLRKICI 147
           P    ++P +   I KN+ ++F N  +L +  P  LL   H +    +   +  +R IC+
Sbjct: 70  PVLVASSPEAAREIMKNQDLNFSNRPNLSI--PRRLLYDNHDVAFAPYGEYWRQIRSICV 129

Query: 148 LELLSAKRVQSFALLRQQEVARLVDRISQSQSHNP---LDLSTFLALYANDVVCRTALGR 207
           L+LLS KRVQSF  +R++E + +V++I Q Q   P   ++L+  L    NDV CR ALG+
Sbjct: 130 LQLLSNKRVQSFRRVREEETSIMVEKIMQLQKTTPTAAVNLTDLLTCLTNDVFCRIALGK 189

Query: 208 GF----SAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGF 267
            +    +  GEYH+  +K+ L E  +LLG   + +  P L +  +F G+  R+    + F
Sbjct: 190 KYGGTTTGDGEYHVRSLKKNLAEFYVLLGISPLWEYIPWLEWTRRFDGVDRRIEEVSRTF 249

Query: 268 DKLFDQVIAEH-LNPERE----ILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILD 327
           D+   +VI EH +  +RE    + +    D VD+LL  Q++      P+    +KA+ILD
Sbjct: 250 DEFLGKVIQEHRVRDKREDTTVVGDTVGLDFVDLLLQFQRENERSSSPVDDLTIKAVILD 309

Query: 328 MFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVK 387
           MF AGTDTT   L+W ++ELI++PRAM+  Q E+R + G +  + ESDL +MPYLKAV+K
Sbjct: 310 MFLAGTDTTVTALEWALSELIKNPRAMKILQKEVRGVAGSKGEIEESDLEKMPYLKAVMK 369

Query: 388 EVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFL 447
           E LRLH P P+ V RE+T D  + GYD+ + TRV +N WAIGRD   W++ E+F PERFL
Sbjct: 370 ESLRLHAPVPLLVPRESTRDTKVLGYDVASGTRVLINCWAIGRDSSVWEESETFLPERFL 429

Query: 448 ESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMT 507
           E+ +DYRG+ FE IPFG+GRR CPG      + ELALA ++H FD++LPNG+   DLDM+
Sbjct: 430 ETSIDYRGMHFELIPFGSGRRGCPGATFAAAIDELALATLVHKFDFKLPNGVRVEDLDMS 489

Query: 508 EVYGVTMHRKAHLDVVAKPY 514
           E  G T+H+K  L VV  P+
Sbjct: 490 EGSGFTIHKKFPLLVVPTPH 507

[Close]

BLAST of Lsi02G004390 vs. ExPASy TrEMBL
Match: A0A5D3BIU1 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004130 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.5e-175
Identity = 327/446 (73.32%), Postives = 366/446 (82.06%), Query Frame = 0


Query: 69  RRLSPSLFPPPLPQIRPHHPFTTRTNPNSHNLIFKNRQISFQNPRSLLLKPPLSLLRSTH 128
           RRLS    P  L Q+    P    ++P     +FK   ++F +   L     +    +  
Sbjct: 65  RRLSQKFGPIFLLQL-GQIPTLVVSSPKIAKQVFKTHDLAFSSRPFLFSAQHIFYNCTDI 124

Query: 129 LLQLHRRCFFPLRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLA 188
           +   +   +  +RKICIL+LLSA+RVQSF+L+RQQEVARLVDRIS S+    ++LS+ L 
Sbjct: 125 VFSPYGSYWRQVRKICILQLLSARRVQSFSLIRQQEVARLVDRISHSKDR--VNLSSLLG 184

Query: 189 LYANDVVCRTALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMK 248
           LYANDV+CR ALGR FSAGGEYHLHG ++LLEE QILLGGFCIGDLFPSLAFLS FTGMK
Sbjct: 185 LYANDVLCRAALGREFSAGGEYHLHGFQKLLEEYQILLGGFCIGDLFPSLAFLSNFTGMK 244

Query: 249 SRLVRTVKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVK 308
           SRLVRT  GFDKLFDQVIAEHL+PERE LEE S+DLVD+LL+IQK+GSDDKIPLTMDNVK
Sbjct: 245 SRLVRTANGFDKLFDQVIAEHLDPEREKLEE-SQDLVDILLEIQKNGSDDKIPLTMDNVK 304

Query: 309 AIILDMFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYL 368
           AIILDMFAAGTDTTFI LDWGMTELI HP AM+RAQ+EIR +VG+RR V+ESDLLEMPYL
Sbjct: 305 AIILDMFAAGTDTTFIALDWGMTELITHPNAMKRAQSEIRRVVGDRRNVTESDLLEMPYL 364

Query: 369 KAVVKEVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFE 428
           KAVVKEVLRLHPPAPV V RETTEDV IEGYDIPAKTRVFVN W IGRDPESWKDPE+FE
Sbjct: 365 KAVVKEVLRLHPPAPVLVPRETTEDVRIEGYDIPAKTRVFVNAWGIGRDPESWKDPETFE 424

Query: 429 PERFLESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEAT 488
           PERFL SEVDY GLDFEFIPFGAGRRICPGI +GI  IELALAQILHSFDWELPNGIEA 
Sbjct: 425 PERFLGSEVDYGGLDFEFIPFGAGRRICPGITMGIATIELALAQILHSFDWELPNGIEAK 484

Query: 489 DLDMTEVYGVTMHRKAHLDVVAKPYF 515
           DLDMTEV+G+TMHRKAHL+ V KPYF
Sbjct: 485 DLDMTEVFGITMHRKAHLEAVPKPYF 506

[Close]

BLAST of Lsi02G004390 vs. ExPASy TrEMBL
Match: A0A1S4DW70 (cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC107990306 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.5e-175
Identity = 327/446 (73.32%), Postives = 366/446 (82.06%), Query Frame = 0


Query: 69  RRLSPSLFPPPLPQIRPHHPFTTRTNPNSHNLIFKNRQISFQNPRSLLLKPPLSLLRSTH 128
           RRLS    P  L Q+    P    ++P     +FK   ++F +   L     +    +  
Sbjct: 65  RRLSQKFGPIFLLQL-GQIPTLVVSSPKIAKQVFKTHDLAFSSRPFLFSAQHIFYNCTDI 124

Query: 129 LLQLHRRCFFPLRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLA 188
           +   +   +  +RKICIL+LLSA+RVQSF+L+RQQEVARLVDRIS S+    ++LS+ L 
Sbjct: 125 VFSPYGSYWRQVRKICILQLLSARRVQSFSLIRQQEVARLVDRISHSKDR--VNLSSLLG 184

Query: 189 LYANDVVCRTALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMK 248
           LYANDV+CR ALGR FSAGGEYHLHG ++LLEE QILLGGFCIGDLFPSLAFLS FTGMK
Sbjct: 185 LYANDVLCRAALGREFSAGGEYHLHGFQKLLEEYQILLGGFCIGDLFPSLAFLSNFTGMK 244

Query: 249 SRLVRTVKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVK 308
           SRLVRT  GFDKLFDQVIAEHL+PERE LEE S+DLVD+LL+IQK+GSDDKIPLTMDNVK
Sbjct: 245 SRLVRTANGFDKLFDQVIAEHLDPEREKLEE-SQDLVDILLEIQKNGSDDKIPLTMDNVK 304

Query: 309 AIILDMFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYL 368
           AIILDMFAAGTDTTFI LDWGMTELI HP AM+RAQ+EIR +VG+RR V+ESDLLEMPYL
Sbjct: 305 AIILDMFAAGTDTTFIALDWGMTELITHPNAMKRAQSEIRRVVGDRRNVTESDLLEMPYL 364

Query: 369 KAVVKEVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFE 428
           KAVVKEVLRLHPPAPV V RETTEDV IEGYDIPAKTRVFVN W IGRDPESWKDPE+FE
Sbjct: 365 KAVVKEVLRLHPPAPVLVPRETTEDVRIEGYDIPAKTRVFVNAWGIGRDPESWKDPETFE 424

Query: 429 PERFLESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEAT 488
           PERFL SEVDY GLDFEFIPFGAGRRICPGI +GI  IELALAQILHSFDWELPNGIEA 
Sbjct: 425 PERFLGSEVDYGGLDFEFIPFGAGRRICPGITMGIATIELALAQILHSFDWELPNGIEAK 484

Query: 489 DLDMTEVYGVTMHRKAHLDVVAKPYF 515
           DLDMTEV+G+TMHRKAHL+ V KPYF
Sbjct: 485 DLDMTEVFGITMHRKAHLEAVPKPYF 506

[Close]

BLAST of Lsi02G004390 vs. ExPASy TrEMBL
Match: A0A5A7UNY6 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001240 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 1.1e-167
Identity = 297/375 (79.20%), Postives = 331/375 (88.27%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTA 199
           +RKICIL+LL+AKRVQSFA +RQQEVARLV RI  S S+NP+DLS  LA+Y NDV+CR A
Sbjct: 135 VRKICILQLLNAKRVQSFAFIRQQEVARLVKRI--SHSNNPVDLSNLLAMYGNDVICRMA 194

Query: 200 LGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFD 259
           LGR FSAGG YHLHGI+ELLEE Q+LL GFC GDLFPSL+F+S  TG+KSRLVRT K FD
Sbjct: 195 LGREFSAGGAYHLHGIQELLEEFQLLLRGFCFGDLFPSLSFISNLTGLKSRLVRTFKRFD 254

Query: 260 KLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGT 319
           KL DQVI EH +PE E L E  +DLVDVLLDIQK+GSDD+ PLTMDNVKAI+LDMF AGT
Sbjct: 255 KLLDQVIVEHQSPEGEKLGE-FKDLVDVLLDIQKNGSDDRFPLTMDNVKAILLDMFIAGT 314

Query: 320 DTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLH 379
           +TTF  LDWGMTELI HP+ MERAQNEIRSIVGE++IV+E D+LEM YLKAVVKEVLRLH
Sbjct: 315 ETTFTALDWGMTELITHPKVMERAQNEIRSIVGEKKIVTEDDVLEMHYLKAVVKEVLRLH 374

Query: 380 PPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEVDY 439
           PPAP+++ RETT++V IEGYDIPAKTRVFVNVW IGRDPESWK+PESFEPERF+E+EVDY
Sbjct: 375 PPAPLALPRETTKNVRIEGYDIPAKTRVFVNVWGIGRDPESWKNPESFEPERFIENEVDY 434

Query: 440 RGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGVT 499
           RGLDFEFIPFG GRRICPGI LGI MIE+A AQILHSFDWELPNGIE  DLDMTEV GVT
Sbjct: 435 RGLDFEFIPFGVGRRICPGITLGIAMIEIAFAQILHSFDWELPNGIEVKDLDMTEVVGVT 494

Query: 500 MHRKAHLDVVAKPYF 515
           MHRKAHL+VVAKPYF
Sbjct: 495 MHRKAHLEVVAKPYF 506

[Close]

BLAST of Lsi02G004390 vs. ExPASy TrEMBL
Match: A0A5D3CT71 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold259G00600 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 4.2e-167
Identity = 320/464 (68.97%), Postives = 361/464 (77.80%), Query Frame = 0


Query: 53  PTISTKATLNWQPSPS-RRLSPSLFPPPLPQIRPHHPFTTRTNPNSHNLIFKNRQISFQN 112
           P I     LN  P    RRLS    P  L Q+    P    ++P      FK   +SF +
Sbjct: 48  PLIGNLHHLNQHPHLCFRRLSQKFGPIILLQL-GQIPTLIISSPKIAKEAFKTHDLSFSS 107

Query: 113 PRSLLLKPPLSLLRSTHL-LQLHRRCFFPLRKICILELLSAKRVQSFALLRQQEVARLVD 172
            R  L     +    T +    +   +  +RKICIL+LLS KRVQSFA +RQQEVARLV+
Sbjct: 108 -RPFLFSAQHTFYNCTDIAFSPYGSYWRRVRKICILQLLSTKRVQSFAFIRQQEVARLVN 167

Query: 173 RISQSQSHNPLDLSTFLALYANDVVCRTALGRGFSAGGEYHLHGIKELLEELQILLGGFC 232
           RI  S S+NP+DLS  LALY NDV+CR ALGR FS GG YHLHGI+ELLEE Q+LL GFC
Sbjct: 168 RI--SHSNNPVDLSNLLALYGNDVICRMALGREFSVGGAYHLHGIQELLEEYQLLLRGFC 227

Query: 233 IGDLFPSLAFLSKFTGMKSRLVRTVKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLD 292
            GDLFPSL+F+SK TGMKSRLVRT K FDKL DQVI EH +PE E L E S+DLVDVLLD
Sbjct: 228 FGDLFPSLSFISKLTGMKSRLVRTFKRFDKLLDQVIVEHQSPEGEKLGE-SKDLVDVLLD 287

Query: 293 IQKDGSDDKIPLTMDNVKAIILDMFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSI 352
           IQK+GSDD  PLTMDNVKAI+LDMF AGT+TTF  LDWGMTELI HP+AMERAQNEIRSI
Sbjct: 288 IQKNGSDDGFPLTMDNVKAILLDMFIAGTETTFTALDWGMTELITHPKAMERAQNEIRSI 347

Query: 353 VGERRIVSESDLLEMPYLKAVVKEVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVN 412
           VGER+IV+E D+LEM YLKAVVKEVLRLHPPAP+++ RETT+DV IEGYDIPAKTRVFVN
Sbjct: 348 VGERKIVTEGDVLEMHYLKAVVKEVLRLHPPAPLALPRETTDDVRIEGYDIPAKTRVFVN 407

Query: 413 VWAIGRDPESWKDPESFEPERFLESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELAL 472
           VW IGRDPESWK+PESFEPERF+E+EVDYRGLDFEFIPFG GRRICPGI +GI MIE+A 
Sbjct: 408 VWGIGRDPESWKNPESFEPERFIENEVDYRGLDFEFIPFGGGRRICPGITMGIAMIEIAF 467

Query: 473 AQILHSFDWELPNGIEATDLDMTEVYGVTMHRKAHLDVVAKPYF 515
           AQILHSFDWELP+GIE  DLDMTEV GVT+HRKAHL+VV KPYF
Sbjct: 468 AQILHSFDWELPSGIEVEDLDMTEVVGVTVHRKAHLEVVPKPYF 506

[Close]

BLAST of Lsi02G004390 vs. ExPASy TrEMBL
Match: A0A5A7VFB1 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold538G00670 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 1.2e-166
Identity = 320/482 (66.39%), Postives = 364/482 (75.52%), Query Frame = 0


Query: 53  PTISTKATLNWQPSPSR-------------------RLSPSLFPPPLPQIRPHHPFTTRT 112
           P + +K   N  PSP +                   RLS    P  L Q+    P    +
Sbjct: 30  PKLKSKPNQNLPPSPPKLPLIGNLHHLNHHPHLCFHRLSQKFGPIILLQL-GQIPTLIIS 89

Query: 113 NPNSHNLIFKNRQISFQNPRSLLLKPPLSLLRSTHL-LQLHRRCFFPLRKICILELLSAK 172
           +P      FK   +SF + R  L     +    T +    +   +  +RKICIL+LLS K
Sbjct: 90  SPKIAKEAFKTHDLSFSS-RPFLFSAQHTFYNCTDIAFSPYGSYWRRVRKICILQLLSTK 149

Query: 173 RVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTALGRGFSAGGEYHL 232
           RVQSFA +RQQEVARLV+RI  S S+NP+DLS  LALY NDV+CR ALGR FS GG YHL
Sbjct: 150 RVQSFAFIRQQEVARLVNRI--SHSNNPVDLSNLLALYGNDVICRMALGREFSVGGAYHL 209

Query: 233 HGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFDKLFDQVIAEHLNP 292
           HGI+ELLEE Q+LL GFC GDLFPSL+F+SK TGMKSRLVRT K FDKL DQVI EH +P
Sbjct: 210 HGIQELLEEYQLLLRGFCFGDLFPSLSFISKLTGMKSRLVRTFKRFDKLLDQVIVEHQSP 269

Query: 293 EREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGTDTTFITLDWGMTE 352
           E E L E S+DLVDVLLDIQK+GSDD  PLTMDNVKAI+LDMF AGT+TTF  LDWGMTE
Sbjct: 270 EGEKLGE-SKDLVDVLLDIQKNGSDDGFPLTMDNVKAILLDMFIAGTETTFTALDWGMTE 329

Query: 353 LIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLHPPAPVSVARETTE 412
           LI HP+AMERAQNEIRSIVGER+IV+E D+LEM YLKAVVKEVLRLHPPAP+++ RETT+
Sbjct: 330 LITHPKAMERAQNEIRSIVGERKIVTEGDVLEMHYLKAVVKEVLRLHPPAPLALPRETTD 389

Query: 413 DVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEVDYRGLDFEFIPFGAG 472
           DV IEGYDIPAKTRVFVNVW IGRDPESWK+PESFEPERF+E+EVDYRGLDFEFIPFG G
Sbjct: 390 DVRIEGYDIPAKTRVFVNVWGIGRDPESWKNPESFEPERFIENEVDYRGLDFEFIPFGGG 449

Query: 473 RRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGVTMHRKAHLDVVAKP 515
           RRICPGI +GI MIE+A AQILHSFDWELP+GIE  DLDMTEV GVT+HRKAHL+VV KP
Sbjct: 450 RRICPGITMGIAMIEIAFAQILHSFDWELPSGIEVEDLDMTEVVGVTVHRKAHLEVVPKP 506

[Close]

BLAST of Lsi02G004390 vs. NCBI nr
Match: XP_038895182.1 (cytochrome P450 71AP13-like [Benincasa hispida])

HSP 1 Score: 640.6 bits (1651), Expect = 1.2e-179
Identity = 334/440 (75.91%), Postives = 368/440 (83.64%), Query Frame = 0


Query: 80  LPQIRPHHPFTTRTNPNSHNLIFKNR------QISFQNPRSLLLKPPLSLLRSTHLLQLH 139
           +P +    P   +    +H+LIF +R      Q  F N   +   P  S  R        
Sbjct: 85  IPNLIISSPKIAKEAFTTHDLIFSSRPFLFSAQHIFYNCTDIAFSPYGSYWRH------- 144

Query: 140 RRCFFPLRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYAND 199
                 +RKICILELLSAKRV+SFAL+RQQEV RLVDRISQS+S   +DLS+FLALYAND
Sbjct: 145 ------VRKICILELLSAKRVESFALIRQQEVTRLVDRISQSKSR--VDLSSFLALYAND 204

Query: 200 VVCRTALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVR 259
           V+CR ALGR FSA GEYHLHGI+ELLEE QILLGGFCIGDLFPSLAFLSKFTGMKSRL+R
Sbjct: 205 VLCRAALGREFSASGEYHLHGIQELLEEYQILLGGFCIGDLFPSLAFLSKFTGMKSRLMR 264

Query: 260 TVKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILD 319
           T K FDKLFDQVIAEHLNPERE ++E S+DLVDVLLDIQK+GS DKI LTMDNVKAIILD
Sbjct: 265 TAKAFDKLFDQVIAEHLNPERENMDE-SKDLVDVLLDIQKNGSYDKISLTMDNVKAIILD 324

Query: 320 MFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVK 379
           MFAAGTDTTFITLDWGMTELI HP  M+RAQNEIRSIVGERRIVSESD+LEMPYLKAVVK
Sbjct: 325 MFAAGTDTTFITLDWGMTELITHPETMKRAQNEIRSIVGERRIVSESDVLEMPYLKAVVK 384

Query: 380 EVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFL 439
           EVLRLHPP PVSV RET EDV IEGYDIPAKTRVFVN+WAIGRDPESWK+PESFEPERFL
Sbjct: 385 EVLRLHPPVPVSVPRETREDVRIEGYDIPAKTRVFVNLWAIGRDPESWKNPESFEPERFL 444

Query: 440 ESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMT 499
           +S+VDY+GLDFEFIPFG GRRICPGI +GI  +ELALAQILHSFDWELP+G+EA DLDMT
Sbjct: 445 DSDVDYKGLDFEFIPFGTGRRICPGIAMGIATVELALAQILHSFDWELPSGVEAKDLDMT 504

Query: 500 EVYGVTMHRKAHLDVVAKPY 514
           EV+G+TMHRKAHL+VVAKPY
Sbjct: 505 EVFGITMHRKAHLEVVAKPY 508

[Close]

BLAST of Lsi02G004390 vs. NCBI nr
Match: XP_016899955.1 (PREDICTED: cytochrome P450 71A1-like [Cucumis melo] >KAA0055280.1 cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYJ99203.1 cytochrome P450 71A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 625.9 bits (1613), Expect = 3.0e-175
Identity = 327/446 (73.32%), Postives = 366/446 (82.06%), Query Frame = 0


Query: 69  RRLSPSLFPPPLPQIRPHHPFTTRTNPNSHNLIFKNRQISFQNPRSLLLKPPLSLLRSTH 128
           RRLS    P  L Q+    P    ++P     +FK   ++F +   L     +    +  
Sbjct: 65  RRLSQKFGPIFLLQL-GQIPTLVVSSPKIAKQVFKTHDLAFSSRPFLFSAQHIFYNCTDI 124

Query: 129 LLQLHRRCFFPLRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLA 188
           +   +   +  +RKICIL+LLSA+RVQSF+L+RQQEVARLVDRIS S+    ++LS+ L 
Sbjct: 125 VFSPYGSYWRQVRKICILQLLSARRVQSFSLIRQQEVARLVDRISHSKDR--VNLSSLLG 184

Query: 189 LYANDVVCRTALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMK 248
           LYANDV+CR ALGR FSAGGEYHLHG ++LLEE QILLGGFCIGDLFPSLAFLS FTGMK
Sbjct: 185 LYANDVLCRAALGREFSAGGEYHLHGFQKLLEEYQILLGGFCIGDLFPSLAFLSNFTGMK 244

Query: 249 SRLVRTVKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVK 308
           SRLVRT  GFDKLFDQVIAEHL+PERE LEE S+DLVD+LL+IQK+GSDDKIPLTMDNVK
Sbjct: 245 SRLVRTANGFDKLFDQVIAEHLDPEREKLEE-SQDLVDILLEIQKNGSDDKIPLTMDNVK 304

Query: 309 AIILDMFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYL 368
           AIILDMFAAGTDTTFI LDWGMTELI HP AM+RAQ+EIR +VG+RR V+ESDLLEMPYL
Sbjct: 305 AIILDMFAAGTDTTFIALDWGMTELITHPNAMKRAQSEIRRVVGDRRNVTESDLLEMPYL 364

Query: 369 KAVVKEVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFE 428
           KAVVKEVLRLHPPAPV V RETTEDV IEGYDIPAKTRVFVN W IGRDPESWKDPE+FE
Sbjct: 365 KAVVKEVLRLHPPAPVLVPRETTEDVRIEGYDIPAKTRVFVNAWGIGRDPESWKDPETFE 424

Query: 429 PERFLESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEAT 488
           PERFL SEVDY GLDFEFIPFGAGRRICPGI +GI  IELALAQILHSFDWELPNGIEA 
Sbjct: 425 PERFLGSEVDYGGLDFEFIPFGAGRRICPGITMGIATIELALAQILHSFDWELPNGIEAK 484

Query: 489 DLDMTEVYGVTMHRKAHLDVVAKPYF 515
           DLDMTEV+G+TMHRKAHL+ V KPYF
Sbjct: 485 DLDMTEVFGITMHRKAHLEAVPKPYF 506

[Close]

BLAST of Lsi02G004390 vs. NCBI nr
Match: XP_011651857.2 (cytochrome P450 71A1 [Cucumis sativus] >KAE8652610.1 hypothetical protein Csa_013309 [Cucumis sativus])

HSP 1 Score: 623.6 bits (1607), Expect = 1.5e-174
Identity = 333/481 (69.23%), Postives = 374/481 (77.75%), Query Frame = 0


Query: 53  PTISTKATLNWQPSPSR-------------------RLSPSLFPPPLPQIRPHHPFTTRT 112
           P + +K   N  PSP +                   RLS    P  L Q+    P    +
Sbjct: 39  PNLKSKPNKNLPPSPPKLPIIGNLHQLGRHPHLSFCRLSQKFGPIILLQL-GQIPTLIIS 98

Query: 113 NPNSHNLIFKNRQISFQNPRSLLLKPPLSLLRSTHLLQLHRRCFFPLRKICILELLSAKR 172
           +P      FK   ++F +   L     +    +  +   +   +  +RKICIL+LLSAKR
Sbjct: 99  SPKIAKQAFKTHDLAFSSRPFLFSAQHIFYNCTDIVFSPYGSYWRQVRKICILQLLSAKR 158

Query: 173 VQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTALGRGFSAGGEYHLH 232
           VQSF+L+RQQEVARLVDRI  S S+N +DLS  L LYANDV+CR+ALGR FSAGGEYHLH
Sbjct: 159 VQSFSLIRQQEVARLVDRI--SHSNNRVDLSNLLGLYANDVLCRSALGREFSAGGEYHLH 218

Query: 233 GIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFDKLFDQVIAEHLNPE 292
           GI++LLEE QILLGGFCIGDLFPSLAFLS FTGM+SRL RT  GFDKLFDQVIAEHLNPE
Sbjct: 219 GIQKLLEEYQILLGGFCIGDLFPSLAFLSNFTGMRSRLARTANGFDKLFDQVIAEHLNPE 278

Query: 293 REILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGTDTTFITLDWGMTEL 352
           RE LEE S+DLVDVLL+IQK+GSDDK+PLTMDNVKAIILDMFAAGTDTTFI LDWGMTEL
Sbjct: 279 REKLEE-SKDLVDVLLEIQKNGSDDKVPLTMDNVKAIILDMFAAGTDTTFIALDWGMTEL 338

Query: 353 IRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLHPPAPVSVARETTED 412
           I HP+AM+RAQ+EIR +VG+RR V+ESD+LEMPYLKAVVKEVLRLHPPAPV V RET ED
Sbjct: 339 ITHPKAMKRAQSEIRRVVGDRRNVTESDVLEMPYLKAVVKEVLRLHPPAPVLVPRETMED 398

Query: 413 VSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEVDYRGLDFEFIPFGAGR 472
           V IEGYDIPAKTRVFVNVW IGRDPESWKDPESFEPERFL S VDY GLDFEF+PFG GR
Sbjct: 399 VRIEGYDIPAKTRVFVNVWGIGRDPESWKDPESFEPERFLGSGVDYGGLDFEFLPFGGGR 458

Query: 473 RICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGVTMHRKAHLDVVAKPY 515
           RICPGI +GIV IELALAQILHSFDWELPNGIEA DLDMTEV+G+TMHRKA L+ VAKPY
Sbjct: 459 RICPGITMGIVTIELALAQILHSFDWELPNGIEAKDLDMTEVFGITMHRKARLEAVAKPY 515

[Close]

BLAST of Lsi02G004390 vs. NCBI nr
Match: XP_031738204.1 (cytochrome P450 71A1-like [Cucumis sativus] >KAE8652612.1 hypothetical protein Csa_013068 [Cucumis sativus])

HSP 1 Score: 600.1 bits (1546), Expect = 1.8e-167
Identity = 322/463 (69.55%), Postives = 365/463 (78.83%), Query Frame = 0


Query: 53  PTISTKATLNWQPSPS-RRLSPSLFPPPLPQIRPHHPFTTRTNPNSHNLIFKNRQISFQN 112
           P I     LN QP    RRLS +  P  L Q+    P    ++P      FK   +SF +
Sbjct: 49  PLIGNLHHLNNQPHLCFRRLSQNFGPIILLQL-GQIPTLIISSPKIAREAFKTHDLSFSS 108

Query: 113 PRSLLLKPPLSLLRSTHLLQLHRRCFFPLRKICILELLSAKRVQSFALLRQQEVARLVDR 172
              L     +    +      +   +  LRK CIL+LLSAKRVQSFA +RQQEVARLV+R
Sbjct: 109 RPFLFSAQHIFYNCTDIAFSPYGSYWRQLRKFCILQLLSAKRVQSFAFIRQQEVARLVNR 168

Query: 173 ISQSQSHNPLDLSTFLALYANDVVCRTALGRGFSAGGEYHLHGIKELLEELQILLGGFCI 232
           IS S +H  +DL++ LA+YANDV+CR ALGR FS GGEYHLHGIKELLEE Q+LLGGF  
Sbjct: 169 ISHSINH--VDLTSLLAMYANDVLCRIALGREFSTGGEYHLHGIKELLEEFQMLLGGFFF 228

Query: 233 GDLFPSLAFLSKFTGMKSRLVRTVKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLDI 292
            DLFPSL+F+S  TG KSRLV+T KGFDKLFDQVIAEH +P RE L E S+DLVDVLLDI
Sbjct: 229 ADLFPSLSFISTLTGTKSRLVKTFKGFDKLFDQVIAEHQSPNREKLGE-SKDLVDVLLDI 288

Query: 293 QKDGSDDKIPLTMDNVKAIILDMFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIV 352
           QK+G +DK  LTMDNVK IILDMF AG+DTT+I LDWGMTELI HP AMERAQ+EIR +V
Sbjct: 289 QKNGFEDKFLLTMDNVKGIILDMFVAGSDTTYIALDWGMTELITHPNAMERAQSEIRRVV 348

Query: 353 GERRIVSESDLLEMPYLKAVVKEVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNV 412
           G+RR V+ESD+LEMPYLKAVVKEVLRLHPPAPVSV RET EDV IEGYDIPAKTRVFVNV
Sbjct: 349 GDRRNVTESDVLEMPYLKAVVKEVLRLHPPAPVSVPRETLEDVRIEGYDIPAKTRVFVNV 408

Query: 413 WAIGRDPESWKDPESFEPERFLESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALA 472
           WAIGRDPESWKDPE+FEPERFLESEVDY+GL+FEFIPFGAGRRICPGI +GIV IELALA
Sbjct: 409 WAIGRDPESWKDPENFEPERFLESEVDYKGLNFEFIPFGAGRRICPGITMGIVTIELALA 468

Query: 473 QILHSFDWELPNGIEATDLDMTEVYGVTMHRKAHLDVVAKPYF 515
           QILHS+DWELP GIEA DLDM+EV+G+TMHRKAHL+VVAKPYF
Sbjct: 469 QILHSYDWELPTGIEAKDLDMSEVFGITMHRKAHLEVVAKPYF 507

[Close]

BLAST of Lsi02G004390 vs. NCBI nr
Match: KAA0055281.1 (cytochrome P450 71A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 599.7 bits (1545), Expect = 2.3e-167
Identity = 297/375 (79.20%), Postives = 331/375 (88.27%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTA 199
           +RKICIL+LL+AKRVQSFA +RQQEVARLV RI  S S+NP+DLS  LA+Y NDV+CR A
Sbjct: 135 VRKICILQLLNAKRVQSFAFIRQQEVARLVKRI--SHSNNPVDLSNLLAMYGNDVICRMA 194

Query: 200 LGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFD 259
           LGR FSAGG YHLHGI+ELLEE Q+LL GFC GDLFPSL+F+S  TG+KSRLVRT K FD
Sbjct: 195 LGREFSAGGAYHLHGIQELLEEFQLLLRGFCFGDLFPSLSFISNLTGLKSRLVRTFKRFD 254

Query: 260 KLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGT 319
           KL DQVI EH +PE E L E  +DLVDVLLDIQK+GSDD+ PLTMDNVKAI+LDMF AGT
Sbjct: 255 KLLDQVIVEHQSPEGEKLGE-FKDLVDVLLDIQKNGSDDRFPLTMDNVKAILLDMFIAGT 314

Query: 320 DTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLH 379
           +TTF  LDWGMTELI HP+ MERAQNEIRSIVGE++IV+E D+LEM YLKAVVKEVLRLH
Sbjct: 315 ETTFTALDWGMTELITHPKVMERAQNEIRSIVGEKKIVTEDDVLEMHYLKAVVKEVLRLH 374

Query: 380 PPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESEVDY 439
           PPAP+++ RETT++V IEGYDIPAKTRVFVNVW IGRDPESWK+PESFEPERF+E+EVDY
Sbjct: 375 PPAPLALPRETTKNVRIEGYDIPAKTRVFVNVWGIGRDPESWKNPESFEPERFIENEVDY 434

Query: 440 RGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGVT 499
           RGLDFEFIPFG GRRICPGI LGI MIE+A AQILHSFDWELPNGIE  DLDMTEV GVT
Sbjct: 435 RGLDFEFIPFGVGRRICPGITLGIAMIEIAFAQILHSFDWELPNGIEVKDLDMTEVVGVT 494

Query: 500 MHRKAHLDVVAKPYF 515
           MHRKAHL+VVAKPYF
Sbjct: 495 MHRKAHLEVVAKPYF 506

[Close]

BLAST of Lsi02G004390 vs. TAIR 10
Match: AT4G31500.1 (cytochrome P450, family 83, subfamily B, polypeptide 1 )

HSP 1 Score: 313.2 bits (801), Expect = 4.0e-85
Identity = 161/371 (43.40%), Postives = 233/371 (62.80%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHN-PLDLSTFLALYANDVVCRT 199
           +RK+C++ L S  RV SF  +R++E  R++D+I ++   +  +DLS  L  + N VVCR 
Sbjct: 125 MRKMCMVNLFSPNRVASFRPVREEECQRMMDKIYKAADQSGTVDLSELLLSFTNCVVCRQ 184

Query: 200 ALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGF 259
           A G+ ++  G   +    ++L E Q LLG     DLFP   FL   TG+ +RL +  K  
Sbjct: 185 AFGKRYNEYGT-EMKRFIDILYETQALLGTLFFSDLFPYFGFLDNLTGLSARLKKAFKEL 244

Query: 260 DKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAG 319
           D    +++ E L+P R   ++ +E  +D+L+ I KD     I  T +NVKA+ILD+   G
Sbjct: 245 DTYLQELLDETLDPNRP--KQETESFIDLLMQIYKD-QPFSIKFTHENVKAMILDIVVPG 304

Query: 320 TDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRL 379
           TDT    + W MT LI++P AM++AQ+E+RS++G++  VSE D+  +PYLKAV+KE LRL
Sbjct: 305 TDTAAAVVVWAMTYLIKYPEAMKKAQDEVRSVIGDKGYVSEEDIPNLPYLKAVIKESLRL 364

Query: 380 HPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKD-PESFEPERFLESE- 439
            P  P+ + RET  D  I GYDIPAKT + VN WA+ RD  +W D P  F PERF+    
Sbjct: 365 EPVIPILLHRETIADAKIGGYDIPAKTIIQVNAWAVSRDTAAWGDNPNEFIPERFMNEHK 424

Query: 440 -VDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEV 499
            VD++G DFE +PFG+GRR+CP + LGI M+E+  A +L+ FDW LP GI+  D+ M  +
Sbjct: 425 GVDFKGQDFELLPFGSGRRMCPAMHLGIAMVEIPFANLLYKFDWSLPKGIKPEDIKMDVM 484

Query: 500 YGVTMHRKAHL 507
            G+ MH+K HL
Sbjct: 485 TGLAMHKKEHL 491

[Close]

BLAST of Lsi02G004390 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 312.8 bits (800), Expect = 5.2e-85
Identity = 155/373 (41.55%), Postives = 236/373 (63.27%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQ-SQSHNPLDLSTFLALYANDVVCRT 199
           LRK+ ++EL S K+  SF  +R++E   LV ++S+ S+  +P++L   L   +  +VCR 
Sbjct: 126 LRKLAVIELFSLKKFNSFRYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSASIVCRL 185

Query: 200 ALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGF 259
           A G+            +++L    + +   F   + FP    L K TG    L       
Sbjct: 186 AFGQNLHESEFIDEDSMEDLASRSEKIQAKFAFSNFFPGGWILDKITGQSKSLNEIFADL 245

Query: 260 DKLFDQVIAEHLNPEREILEESSEDLVDVLLD-IQKDGSDDKIPLTMDNVKAIILDMFAA 319
           D  F+QV+ +HL P R++LE  + D+VDV++D + K   D    LT D++K II D+F A
Sbjct: 246 DGFFNQVLDDHLKPGRKVLE--TPDVVDVMIDMMNKQSQDGSFKLTTDHIKGIISDIFLA 305

Query: 320 GTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERR-IVSESDLLEMPYLKAVVKEVL 379
           G +T+  T+ W MTELIR+PR M++ Q+E+R+++GE+R  ++E DL ++ Y K V+KE  
Sbjct: 306 GVNTSATTILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLNYFKLVIKETF 365

Query: 380 RLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLESE 439
           RLHP AP+ + RE    + I+GYDIP KT++ VNV+AIGRDP+ W++PE F+PERF++S 
Sbjct: 366 RLHPAAPLLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEEFKPERFVDSS 425

Query: 440 VDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVY 499
           VDYRGL+FE +PFG+GRRICPG+ +GI  +EL L  +L+ FDW LP G    D+D+ E  
Sbjct: 426 VDYRGLNFELLPFGSGRRICPGMTMGIATVELGLLNLLYFFDWGLPEGRTVKDIDLEEEG 485

Query: 500 GVTMHRKAHLDVV 510
            + + +K  L++V
Sbjct: 486 AIIIGKKVSLELV 496

[Close]

BLAST of Lsi02G004390 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 308.5 bits (789), Expect = 9.9e-84
Identity = 161/377 (42.71%), Postives = 239/377 (63.40%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQ-SHNPLDLS-TFLALYANDVVCR 199
           +RK+ ++EL S+K+VQSF  +R++EV  +V ++S+S    +P+DLS TF +L A+ ++CR
Sbjct: 126 VRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTAS-IICR 185

Query: 200 TALGRGFSAGG-EYHLHGIKELLEELQILLGGFCIGDLFPS--LAFLSKFTGMKSRLVRT 259
            ALG+ F+  G       I+EL+ E    LG F   D FP     F+        ++ + 
Sbjct: 186 VALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINKV 245

Query: 260 VKGFDKLFDQVIAEHLNPEREILEESSEDLVDVLLD-IQKDGSDDKIPLTMDNVKAIILD 319
            K  D  +  VI +HL PE       ++D+V ++LD I K    D   L MDN+KAI++D
Sbjct: 246 FKELDAFYQHVIDDHLKPE----GRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMD 305

Query: 320 MFAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVG-ERRIVSESDLLEMPYLKAVV 379
           +F AG DT+ +T+ W MTELIR+PR M++AQ  IR+ +G ++  ++E DL ++ YL  ++
Sbjct: 306 VFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHIL 365

Query: 380 KEVLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERF 439
           KE  RLHP  P  V RET   + I+GYDIP KT++ +NVW IGRDP+ W DPE F PERF
Sbjct: 366 KETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERF 425

Query: 440 LESEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDM 499
             S VD+RG  F+ +PFG+GRRICPG+ + I  +ELAL  +L+ FDW +P+G +  D+DM
Sbjct: 426 ANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDM 485

Query: 500 TEVYGVTMHRKAHLDVV 510
            E   +++ +K  L +V
Sbjct: 486 EEAGNISIVKKIPLQLV 497

[Close]

BLAST of Lsi02G004390 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 306.2 bits (783), Expect = 4.9e-83
Identity = 157/375 (41.87%), Postives = 240/375 (64.00%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSH-NPLDLSTFLALYANDVVCRT 199
           +RK+C+ EL S K+V S   ++ +EV +++D I++S S  NP++L+         VVCRT
Sbjct: 125 VRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRT 184

Query: 200 ALGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAF-LSKFTGMKSRLVRTVKG 259
           A G  F  G   +     +++ E   +LG F   D  P + + +   TG++ R  R+ + 
Sbjct: 185 AFGVSFE-GTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRD 244

Query: 260 FDKLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGS---DDKIPLTMDNVKAIILDM 319
            +  F+Q+   H    +E  +E +ED VD+LL ++K+ +   +DK  LT +++KAI+LD+
Sbjct: 245 LNAFFEQMFDLH----KEGKKEGNEDFVDLLLRLEKEEAVLGNDK--LTRNHIKAILLDV 304

Query: 320 FAAGTDTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKE 379
             AG DT+ IT+ W MTEL R+PR M++ Q+EIR+ +G R ++S  D+ ++ YLK V+KE
Sbjct: 305 LLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKE 364

Query: 380 VLRLHPPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESWKDPESFEPERFLE 439
             RLHP  P+ + RE   +  I GY IP KTR+ VNVWAIGRDP++WKDPE F PERF++
Sbjct: 365 TWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMD 424

Query: 440 SEVDYRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTE 499
           + +D +G  FE +PFG GRRICP I +G  M+E  LA +L+ FDW+LP G+E  D+D+ E
Sbjct: 425 NNIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEE 484

Query: 500 VYGVTMHRKAHLDVV 510
             G+T+++K  L +V
Sbjct: 485 APGLTVNKKNELLLV 492

[Close]

BLAST of Lsi02G004390 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 304.3 bits (778), Expect = 1.9e-82
Identity = 158/375 (42.13%), Postives = 235/375 (62.67%), Query Frame = 0


Query: 140 LRKICILELLSAKRVQSFALLRQQEVARLVDRISQSQSHNPLDLSTFLALYANDVVCRTA 199
           ++ +C++ LLS K V+SF  +R++E+  ++ +I +S S  P ++S  L    NDV+CR A
Sbjct: 125 MKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSSS-LPFNVSKVLECLTNDVICRVA 184

Query: 200 LGRGFSAGGEYHLHGIKELLEELQILLGGFCIGDLFPSLAFLSKFTGMKSRLVRTVKGFD 259
           LGR +  GGE      K+L + L  LLG F IG   P LA++    G  ++L +  K  D
Sbjct: 185 LGRKY--GGE---TDFKKLTDRLSELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLD 244

Query: 260 KLFDQVIAEHLNPEREILEESSEDLVDVLLDIQKDGSDDKIPLTMDNVKAIILDMFAAGT 319
             F++V+ +H + +R        DL+D LL ++++ S     +   ++KAI LD+F  G+
Sbjct: 245 DFFEKVVQDHEDGDR----RDGTDLIDALLRVKREKSPG-FEIERVSIKAITLDVFVGGS 304

Query: 320 DTTFITLDWGMTELIRHPRAMERAQNEIRSIVGERRIVSESDLLEMPYLKAVVKEVLRLH 379
           DT+F  L+W MTEL+RHP+++ R Q E+R+I   +  VSE D+  M YLKAV+KE LRLH
Sbjct: 305 DTSFTLLEWAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLH 364

Query: 380 PPAPVSVARETTEDVSIEGYDIPAKTRVFVNVWAIGRDPESW-KDPESFEPERFLESEVD 439
           PP P+    E+TEDV +  Y IPA T+V +N WAIGR+  +W  D E F+PER L++ VD
Sbjct: 365 PPFPMMAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERHLDTSVD 424

Query: 440 YRGLDFEFIPFGAGRRICPGIKLGIVMIELALAQILHSFDWELPNGIEATDLDMTEVYGV 499
           +RG +FE +PFGAGRRICP +   +V+ E+ LA ++H FDW+LP   +    D+ E  G 
Sbjct: 425 FRGQNFELLPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKEDKTDVAESSGF 484

Query: 500 TMHRKAHLDVVAKPY 514
           ++HR+  L  VA PY
Sbjct: 485 SVHREFPLYAVASPY 488

The following BLAST results are available for this feature:

BLAST of Lsi02G004390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q721	1.6e-139	65.60	Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1	[more]
P24465	6.2e-99	49.87	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
A0A068Q6L2	1.4e-98	49.87	Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1	[more]
O81970	2.9e-96	46.88	Cytochrome P450 71A9 OS=Glycine max OX=3847 GN=CYP71A9 PE=2 SV=1	[more]
O04164	6.7e-93	42.05	Cytochrome P450 71A6 (Fragment) OS=Nepeta racemosa OX=54731 GN=CYP71A6 PE=2 SV=1	[more]

BLAST of Lsi02G004390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BIU1	1.5e-175	73.32	Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S4DW70	1.5e-175	73.32	cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC107990306 PE=3 SV=1	[more]
A0A5A7UNY6	1.1e-167	79.20	Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A5D3CT71	4.2e-167	68.97	Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7VFB1	1.2e-166	66.39	Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]

BLAST of Lsi02G004390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895182.1	1.2e-179	75.91	cytochrome P450 71AP13-like [Benincasa hispida]	[more]
XP_016899955.1	3.0e-175	73.32	PREDICTED: cytochrome P450 71A1-like [Cucumis melo] >KAA0055280.1 cytochrome P45...	[more]
XP_011651857.2	1.5e-174	69.23	cytochrome P450 71A1 [Cucumis sativus] >KAE8652610.1 hypothetical protein Csa_01...	[more]
XP_031738204.1	1.8e-167	69.55	cytochrome P450 71A1-like [Cucumis sativus] >KAE8652612.1 hypothetical protein C...	[more]
KAA0055281.1	2.3e-167	79.20	cytochrome P450 71A1-like [Cucumis melo var. makuwa]	[more]

BLAST of Lsi02G004390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31500.1	4.0e-85	43.40	cytochrome P450, family 83, subfamily B, polypeptide 1	[more]
AT3G26210.1	5.2e-85	41.55	cytochrome P450, family 71, subfamily B, polypeptide 23	[more]
AT1G13080.1	9.9e-84	42.71	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT3G26310.1	4.9e-83	41.87	cytochrome P450, family 71, subfamily B, polypeptide 35	[more]
AT3G48280.1	1.9e-82	42.13	cytochrome P450, family 71, subfamily A, polypeptide 25	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 317..334 score: 39.95 coord: 370..381 score: 48.01 coord: 447..456 score: 60.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 88..503 e-value: 2.2E-91 score: 306.9
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 369..387 score: 35.21 coord: 446..456 score: 60.52 coord: 306..323 score: 39.6 coord: 456..479 score: 30.04 coord: 410..434 score: 42.17 coord: 326..352 score: 32.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 80..513 e-value: 2.6E-101 score: 341.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 87..512
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..83
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..70
None	No IPR available	PANTHER	PTHR24298:SF701	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 140..514
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 140..514
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 449..458

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G004390.1	Lsi02G004390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen