Lsi02G003640 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G003640
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	BHLH domain-containing protein
Location	chr02: 3105894 .. 3106817 (-)
RNA-Seq Expression	Lsi02G003640
Synteny	Lsi02G003640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTGTCAATGGTGAAACTTCTTCTTCTTCAAAACTAGATCGCAAAACTGTCGAAAAAAATAGAAGAATTCATATGAAATCTCTCTGTTCTAAGCTTTTTAATCTTGTTCCTCCCTCCCATTTCAAAATCTCTAAGGTTTTCTCTCTCTCTTTGTCCTCAAACTCCATCTCACGACCACTCCCTTCGTTAAATAATCGCATGTTTTAGTCTTTAACGTTCAAGTTTAATTTCCTTTTCAATTTAGTTACTTTTGCTAATTGAAAAATAAAATATTCAAACAACATGAACTACTAAAACATGTATATATATATATTTCTCAACTCGATTGTTTAATTCTCTCCCGTGTCTGTGTTGTATGTGTGTTTATTTTGTTTCACAGGAGTTATTATCGCAACAAAATCAAATCTCTTATGTAATTGCTTACATAAATGAATTGAAAGAAAGAGTGGAGAATTTGGAGAAAAGGAGACAAGCTTTACAATTACGTAATCATTCTCATAATCATGATTATCAAGCTAATATCGACAGGGTTAGTGCAACCAGGATGTTAGCTAATAATGACGATTTTACATTGCCTGTGGTCGATTTAAGAGATTTAGGCAATGACATTGTTCACATAATGCTAATTAGTAGTCTCAATAGAAGCTTCATGCTCCACCAAATTATTAGTGTCGTCGAAGAGGAAGGCGGCCAAGTCGTCAATGCTAGCTTATCAACGGTTGGTAACAAGGTCTTTCACTCCCTTCACATTGAAGTAAGTAACGTGTGTAAATATTTAAAAAGTCATTCCAAATAAAACGTAAGATGACGTACGTAATAATCATATAATCTTGTAATTTGCAGGCAAAAATTTCGAGAGTTGGAATAGAGACTTCGAGAGTACAACAAAGATTGTTGAATTTGGTATACCAAAATTACAAATAA

mRNA sequence

GCTGTCAATGGTGAAACTTCTTCTTCTTCAAAACTAGATCGCAAAACTGTCGAAAAAAATAGAAGAATTCATATGAAATCTCTCTGTTCTAAGCTTTTTAATCTTGTTCCTCCCTCCCATTTCAAAATCTCTAAGGAGTTATTATCGCAACAAAATCAAATCTCTTATGTAATTGCTTACATAAATGAATTGAAAGAAAGAGTGGAGAATTTGGAGAAAAGGAGACAAGCTTTACAATTACGTAATCATTCTCATAATCATGATTATCAAGCTAATATCGACAGGGTTAGTGCAACCAGGATGTTAGCTAATAATGACGATTTTACATTGCCTGTGGTCGATTTAAGAGATTTAGGCAATGACATTGTTCACATAATGCTAATTAGTAGTCTCAATAGAAGCTTCATGCTCCACCAAATTATTAGTGTCGTCGAAGAGGAAGGCGGCCAAGTCGTCAATGCTAGCTTATCAACGGTTGGTAACAAGGTCTTTCACTCCCTTCACATTGAAGCAAAAATTTCGAGAGTTGGAATAGAGACTTCGAGAGTACAACAAAGATTGTTGAATTTGGTATACCAAAATTACAAATAA

Coding sequence (CDS)

GCTGTCAATGGTGAAACTTCTTCTTCTTCAAAACTAGATCGCAAAACTGTCGAAAAAAATAGAAGAATTCATATGAAATCTCTCTGTTCTAAGCTTTTTAATCTTGTTCCTCCCTCCCATTTCAAAATCTCTAAGGAGTTATTATCGCAACAAAATCAAATCTCTTATGTAATTGCTTACATAAATGAATTGAAAGAAAGAGTGGAGAATTTGGAGAAAAGGAGACAAGCTTTACAATTACGTAATCATTCTCATAATCATGATTATCAAGCTAATATCGACAGGGTTAGTGCAACCAGGATGTTAGCTAATAATGACGATTTTACATTGCCTGTGGTCGATTTAAGAGATTTAGGCAATGACATTGTTCACATAATGCTAATTAGTAGTCTCAATAGAAGCTTCATGCTCCACCAAATTATTAGTGTCGTCGAAGAGGAAGGCGGCCAAGTCGTCAATGCTAGCTTATCAACGGTTGGTAACAAGGTCTTTCACTCCCTTCACATTGAAGCAAAAATTTCGAGAGTTGGAATAGAGACTTCGAGAGTACAACAAAGATTGTTGAATTTGGTATACCAAAATTACAAATAA

Protein sequence

AVNGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKERVENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDIVHIMLISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSRVQQRLLNLVYQNYK

Homology

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BLAST of Lsi02G003640 vs. ExPASy Swiss-Prot
Match: F4JIJ7 (Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.3e-14
Identity = 66/196 (33.67%), Postives = 106/196 (54.08%), Query Frame = 0


Query: 3   NGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 62
           +  T  S  +DRKTVEKNRR+ MKSL S+L +L+P      S E L+  +Q+     YI 
Sbjct: 5   HSNTGQSRSVDRKTVEKNRRMQMKSLYSELISLLP---HHSSTEPLTLPDQLDEAANYIK 64

Query: 63  ELKERVENLEKRRQ------ALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLR 122
           +L+  VE   +R++       L+  N   +    +++D VS  R         LP ++++
Sbjct: 65  KLQVNVEKKRERKRNLVATTTLEKLNSVGSSSVSSSVD-VSVPR--------KLPKIEIQ 124

Query: 123 DLGNDIVHIMLISSLNRSFMLHQIISVVEEE-GGQVVNASLSTVGNKVFHSLHIEAKISR 182
           + G+ I HI L++SL   FM  +II V+ EE G ++ +A  S V + VFH+LH + +   
Sbjct: 125 ETGS-IFHIFLVTSLEHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEHD 184

Query: 183 VGIETSRVQQRLLNLV 192
            G   S++ +RL  +V
Sbjct: 185 YGAR-SQIPERLEKIV 186

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BLAST of Lsi02G003640 vs. ExPASy Swiss-Prot
Match: Q9XIJ1 (Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-12
Identity = 60/193 (31.09%), Postives = 108/193 (55.96%), Query Frame = 0


Query: 4   GETSSSSKLDRKTV-EKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 63
           GE S+SS  +++ + EK RR+ MK L S L + V P+      +L+ Q       ++Y+ 
Sbjct: 8   GEGSASSLREQRNLREKERRMRMKHLFSILSSHVSPTRRLPVPQLIDQ------AVSYMI 67

Query: 64  ELKERVENL-EKRRQAL--QLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLG 123
           +LKE+V  L E +R+ L  +++N S                         LP + +R L 
Sbjct: 68  QLKEKVNYLNEMKRRMLGGEVKNRSEGSS--------------------LLPKLSIRSL- 127

Query: 124 NDIVHIMLISSLN-RSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGI 183
           + I+ + L+  LN +  MLH+++SV EEEG QV++A+L  + ++ F+++  +A I R+GI
Sbjct: 128 DSIIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGI 173

Query: 184 ETSRVQQRLLNLV 192
           + SR+++RL +++
Sbjct: 188 DPSRIEERLRDII 173

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BLAST of Lsi02G003640 vs. ExPASy Swiss-Prot
Match: F4I4E1 (Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.4e-11
Identity = 57/191 (29.84%), Postives = 104/191 (54.45%), Query Frame = 0


Query: 4   GETSSSSKLDRKTV-EKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 63
           GE +SSS  +++ + EK+RR+ MK L S L + V P+        L   + I    +Y+ 
Sbjct: 8   GEGNSSSLREQRNLREKDRRMRMKHLFSILSSHVSPT------RKLPVPHLIDQATSYMI 67

Query: 64  ELKERVENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDI 123
           +LKE V  L+++++ L L+    N                     F LP + +R   +  
Sbjct: 68  QLKENVNYLKEKKRTL-LQGELGN----------------LYEGSFLLPKLSIRS-RDST 127

Query: 124 VHIMLISSLN-RSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETS 183
           + + LI  LN +  MLH+++S+ EEEG QV++A+L  + ++  +++  +A ISR+GI+ S
Sbjct: 128 IEMNLIMDLNMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPS 174

Query: 184 RVQQRLLNLVY 193
           R+++R+  ++Y
Sbjct: 188 RIEERVRKIIY 174

[Close]

BLAST of Lsi02G003640 vs. ExPASy Swiss-Prot
Match: Q0JFZ0 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=39947 GN=IRO2 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.6e-05
Identity = 52/197 (26.40%), Postives = 99/197 (50.25%), Query Frame = 0


Query: 3   NGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPS-HFKISKELLSQQNQISYVIAYI 62
           +G + S  KL     E++RR  +  L S L  L+P + H K     LS    +S V+ YI
Sbjct: 62  SGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTK-----LSIPTTVSRVLKYI 121

Query: 63  NELKERVENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGND 122
            EL+++VENLE++++ L   + ++          V  +++++      +    + D+   
Sbjct: 122 PELQKQVENLERKKKELTTTSTTNCK------PGVLGSQLMSEGMAPIVSATCINDM-EI 181

Query: 123 IVHIMLISSLNRSFM-LHQIISVVEEEGGQVVNASLST-VGNKVFHSLHIEAKISRVGIE 182
           +V + L+S++  S + L + I V+E EG   +++S S+  GN+ F+S+H++     +  E
Sbjct: 182 MVQVSLLSNVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEE 241

Query: 183 TSRVQQRLLNLVYQNYK 197
                +RL  +V    K
Sbjct: 242 CPAFCERLEKVVRNKAK 246

[Close]

BLAST of Lsi02G003640 vs. ExPASy Swiss-Prot
Match: A2WZ60 (Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=39946 GN=IRO2 PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.4e-05
Identity = 50/196 (25.51%), Postives = 98/196 (50.00%), Query Frame = 0


Query: 3   NGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 62
           +G + S  KL     E++RR  +  L S L  L+P +      + LS    +S V+ YI 
Sbjct: 62  SGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDAD---HTKKLSIPTTVSRVLKYIP 121

Query: 63  ELKERVENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDI 122
           EL+++VENLE++++ L   + ++          V  +++++      +    + D+   +
Sbjct: 122 ELQKQVENLERKKKELTTTSTTNCK------PGVLGSQLMSEGMAPIVSATCINDM-EIM 181

Query: 123 VHIMLISSLNRSFM-LHQIISVVEEEGGQVVNASLST-VGNKVFHSLHIEAKISRVGIET 182
           V + L+S++  S + L + I V+E EG   +++S S+  GN+ F+S+H++     +  E 
Sbjct: 182 VQVSLLSNVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEEC 241

Query: 183 SRVQQRLLNLVYQNYK 197
               +RL  +V    K
Sbjct: 242 PAFCERLEKVVRNKAK 247

[Close]

BLAST of Lsi02G003640 vs. ExPASy TrEMBL
Match: A0A1S3AW43 (uncharacterized protein LOC103483440 OS=Cucumis melo OX=3656 GN=LOC103483440 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 148/192 (77.08%), Postives = 169/192 (88.02%), Query Frame = 0


Query: 4   GETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINE 63
           G +SSS++LDRKT+EKNRR HMKSLCS LFNLVPPSHFKI KEL+SQQNQI +VIAYINE
Sbjct: 9   GTSSSSAELDRKTMEKNRRSHMKSLCSNLFNLVPPSHFKIPKELISQQNQICHVIAYINE 68

Query: 64  LKERVENLEKRRQALQLRNH-SHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDI 123
           LKERVENLEKR+QAL+L+ H SH HD QAN           NN+D TLP++++ DLGNDI
Sbjct: 69  LKERVENLEKRKQALRLQTHSSHIHD-QAN-----------NNNDVTLPIIEINDLGNDI 128

Query: 124 VHIMLISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSR 183
           VHIMLISS+NRSFMLHQIISV+EEEGGQVVNA LST+GNKVFHSLHIEAKISR+GIETSR
Sbjct: 129 VHIMLISSVNRSFMLHQIISVIEEEGGQVVNAGLSTIGNKVFHSLHIEAKISRIGIETSR 188

Query: 184 VQQRLLNLVYQN 195
           V++RL+NLVYQN
Sbjct: 189 VKRRLVNLVYQN 188

[Close]

BLAST of Lsi02G003640 vs. ExPASy TrEMBL
Match: A0A6J1JY79 (transcription factor bHLH168-like OS=Cucurbita maxima OX=3661 GN=LOC111489950 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.2e-55
Identity = 120/186 (64.52%), Postives = 154/186 (82.80%), Query Frame = 0


Query: 8   SSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKER 67
           +S KLDRKTVEKNRRIHMKSLCSKLF+LVP SH+KISK+LLSQQ QISYVIAYINELKER
Sbjct: 39  ASFKLDRKTVEKNRRIHMKSLCSKLFDLVPSSHYKISKDLLSQQTQISYVIAYINELKER 98

Query: 68  VENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDIVHIML 127
           VE+LEKR++ALQL+ HS                 L+ ++D T P+V++++L + IVH+ L
Sbjct: 99  VESLEKRKEALQLQIHS----------------QLSTSNDSTFPIVEVKELCSGIVHVTL 158

Query: 128 ISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSRVQQRL 187
           ISS++R+ ML++II V+EEEGG+VV+AS ST+G+K+FHSLHIEAKISRVGIETSR+++RL
Sbjct: 159 ISSVHRNVMLNEIIVVIEEEGGEVVSASFSTLGDKIFHSLHIEAKISRVGIETSRIEKRL 208

Query: 188 LNLVYQ 194
            NL+ +
Sbjct: 219 ENLIIE 208

[Close]

BLAST of Lsi02G003640 vs. ExPASy TrEMBL
Match: A0A6J1GQZ4 (transcription factor bHLH168-like OS=Cucurbita moschata OX=3662 GN=LOC111456691 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.1e-54
Identity = 122/186 (65.59%), Postives = 150/186 (80.65%), Query Frame = 0


Query: 8   SSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKER 67
           +S KLDRK VEKNRRIHMKSLCSKLFNLVP SH+KISK+LLSQQ QISYVIAYI ELKER
Sbjct: 10  ASIKLDRKIVEKNRRIHMKSLCSKLFNLVPSSHYKISKDLLSQQTQISYVIAYIKELKER 69

Query: 68  VENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDIVHIML 127
           VE+LEKR++ALQL+ HS                 L  + D T P+V++R+L + IVH+ L
Sbjct: 70  VESLEKRKEALQLQIHS----------------QLPTSSDSTFPIVEVRELCSGIVHVTL 129

Query: 128 ISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSRVQQRL 187
           ISS++R+ ML++II VVEEEGG+VV+AS ST+G+KVFHSLHIEAKISRVGIETSRV++RL
Sbjct: 130 ISSVDRNVMLNEIIGVVEEEGGEVVSASFSTLGDKVFHSLHIEAKISRVGIETSRVEKRL 179

Query: 188 LNLVYQ 194
            +L+ +
Sbjct: 190 KHLIIE 179

[Close]

BLAST of Lsi02G003640 vs. ExPASy TrEMBL
Match: A0A6J1C669 (transcription factor bHLH162-like OS=Momordica charantia OX=3673 GN=LOC111008798 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 1.3e-44
Identity = 103/163 (63.19%), Postives = 128/163 (78.53%), Query Frame = 0


Query: 1   AVNGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAY 60
           A  G   +S KLDRKT+EKNRR+HMKSLCSKL +L+PPSHFKISK+LLSQQNQISYVIAY
Sbjct: 2   AKRGGAETSLKLDRKTIEKNRRVHMKSLCSKLVSLIPPSHFKISKDLLSQQNQISYVIAY 61

Query: 61  INELKERVENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGN 120
           INELKERVE LEK+++AL+  +H H+H          A+R        TLPVV+LRDLG+
Sbjct: 62  INELKERVEKLEKKKRALEFTHHHHHH---------QASRSPGGGGS-TLPVVELRDLGS 121

Query: 121 DIVHIMLISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKV 164
            ++ +MLIS LNR+F+L Q+I++VEEEGGQVVNAS ST+G K+
Sbjct: 122 -VIEVMLISDLNRNFLLCQVIAIVEEEGGQVVNASFSTLGGKI 153

[Close]

BLAST of Lsi02G003640 vs. ExPASy TrEMBL
Match: A0A6P3Z232 (transcription factor bHLH162-like OS=Ziziphus jujuba OX=326968 GN=LOC107406380 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 6.2e-42
Identity = 98/190 (51.58%), Postives = 139/190 (73.16%), Query Frame = 0


Query: 3   NGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 62
           N  +S S KLDRKTVE+NRRIHMK LC KL +L+PP HFK SK++LSQQ+Q++    YI 
Sbjct: 7   NSSSSESLKLDRKTVERNRRIHMKGLCFKLASLIPPHHFKPSKDMLSQQDQLNVAACYIK 66

Query: 63  ELKERVENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDI 122
           +L E++E ++ +++A      + N ++  NID +       N     LPV++L+DLG+ I
Sbjct: 67  QLSEKIEKMKLKKEAAIKAKEASNGNF--NIDAI-------NMIGIRLPVIELKDLGSSI 126

Query: 123 VHIMLISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSR 182
             ++LIS L ++FML+++ISV+EEEG +VV+AS STVG+KVFHS+H + KISRVG+ETSR
Sbjct: 127 -EVILISGLKKNFMLYEVISVLEEEGAEVVSASFSTVGDKVFHSVHAQVKISRVGVETSR 186

Query: 183 VQQRLLNLVY 193
           V QRL +L+Y
Sbjct: 187 VWQRLQDLIY 186

[Close]

BLAST of Lsi02G003640 vs. NCBI nr
Match: XP_008438283.1 (PREDICTED: uncharacterized protein LOC103483440 [Cucumis melo])

HSP 1 Score: 275.8 bits (704), Expect = 2.9e-70
Identity = 148/192 (77.08%), Postives = 169/192 (88.02%), Query Frame = 0


Query: 4   GETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINE 63
           G +SSS++LDRKT+EKNRR HMKSLCS LFNLVPPSHFKI KEL+SQQNQI +VIAYINE
Sbjct: 9   GTSSSSAELDRKTMEKNRRSHMKSLCSNLFNLVPPSHFKIPKELISQQNQICHVIAYINE 68

Query: 64  LKERVENLEKRRQALQLRNH-SHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDI 123
           LKERVENLEKR+QAL+L+ H SH HD QAN           NN+D TLP++++ DLGNDI
Sbjct: 69  LKERVENLEKRKQALRLQTHSSHIHD-QAN-----------NNNDVTLPIIEINDLGNDI 128

Query: 124 VHIMLISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSR 183
           VHIMLISS+NRSFMLHQIISV+EEEGGQVVNA LST+GNKVFHSLHIEAKISR+GIETSR
Sbjct: 129 VHIMLISSVNRSFMLHQIISVIEEEGGQVVNAGLSTIGNKVFHSLHIEAKISRIGIETSR 188

Query: 184 VQQRLLNLVYQN 195
           V++RL+NLVYQN
Sbjct: 189 VKRRLVNLVYQN 188

[Close]

BLAST of Lsi02G003640 vs. NCBI nr
Match: XP_031737508.1 (transcription factor bHLH162-like [Cucumis sativus])

HSP 1 Score: 251.1 bits (640), Expect = 7.7e-63
Identity = 145/235 (61.70%), Postives = 166/235 (70.64%), Query Frame = 0


Query: 3   NGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISK----------------- 62
           +GETSSSSKLDRKT+EKNRRIHMKSLCS LFNL+PPSHFKI K                 
Sbjct: 6   HGETSSSSKLDRKTIEKNRRIHMKSLCSNLFNLLPPSHFKIPKVDLSLSLNFLIIPVIYL 65

Query: 63  --------------------------ELLSQQNQISYVIAYINELKERVENLEKRRQALQ 122
                                     EL+SQQNQI YVI YINELKERV+NLEKR+QALQ
Sbjct: 66  KTNIWVMRYINVLDVHACIFYLFVLQELISQQNQICYVITYINELKERVKNLEKRKQALQ 125

Query: 123 LRNH-SHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDIVHIMLISSLNRSFMLH 182
           L+ H SH H+   NI            +D T P++++ DLGNDI+HI+LISSLNRSF LH
Sbjct: 126 LQTHSSHVHNQANNI-----------VNDVTWPLIEINDLGNDIIHIVLISSLNRSFTLH 185

Query: 183 QIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSRVQQRLLNLVYQ 194
           QIISV+EEEGGQVVNASLST+GNKVFHSLHIEAKISR+GIETSRV++RL+NLVYQ
Sbjct: 186 QIISVIEEEGGQVVNASLSTIGNKVFHSLHIEAKISRIGIETSRVKRRLMNLVYQ 229

[Close]

BLAST of Lsi02G003640 vs. NCBI nr
Match: XP_022994121.1 (transcription factor bHLH168-like [Cucurbita maxima])

HSP 1 Score: 225.3 bits (573), Expect = 4.5e-55
Identity = 120/186 (64.52%), Postives = 154/186 (82.80%), Query Frame = 0


Query: 8   SSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKER 67
           +S KLDRKTVEKNRRIHMKSLCSKLF+LVP SH+KISK+LLSQQ QISYVIAYINELKER
Sbjct: 39  ASFKLDRKTVEKNRRIHMKSLCSKLFDLVPSSHYKISKDLLSQQTQISYVIAYINELKER 98

Query: 68  VENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDIVHIML 127
           VE+LEKR++ALQL+ HS                 L+ ++D T P+V++++L + IVH+ L
Sbjct: 99  VESLEKRKEALQLQIHS----------------QLSTSNDSTFPIVEVKELCSGIVHVTL 158

Query: 128 ISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSRVQQRL 187
           ISS++R+ ML++II V+EEEGG+VV+AS ST+G+K+FHSLHIEAKISRVGIETSR+++RL
Sbjct: 159 ISSVHRNVMLNEIIVVIEEEGGEVVSASFSTLGDKIFHSLHIEAKISRVGIETSRIEKRL 208

Query: 188 LNLVYQ 194
            NL+ +
Sbjct: 219 ENLIIE 208

[Close]

BLAST of Lsi02G003640 vs. NCBI nr
Match: XP_023542199.1 (transcription factor bHLH168-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.6 bits (571), Expect = 7.7e-55
Identity = 122/186 (65.59%), Postives = 151/186 (81.18%), Query Frame = 0


Query: 8   SSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKER 67
           +S KLDRKTVEKNRRIHMKSLCSKLFNLVP SH+KISK+LLSQQ QISYVIAYINELKER
Sbjct: 10  ASFKLDRKTVEKNRRIHMKSLCSKLFNLVPSSHYKISKDLLSQQTQISYVIAYINELKER 69

Query: 68  VENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDIVHIML 127
           VE+LEK+++ALQL+ HS                 L  + D T P+V++R+L + IVH+ L
Sbjct: 70  VESLEKKKEALQLQIHS----------------QLPTSSDSTFPIVEVRELCSGIVHVTL 129

Query: 128 ISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSRVQQRL 187
           ISS++R+ ML++II VVEEEGG+VV+AS ST+G+KVFHSLHIEAKI RVGIETSRV++RL
Sbjct: 130 ISSVDRNVMLNEIIGVVEEEGGEVVSASFSTLGDKVFHSLHIEAKIPRVGIETSRVEKRL 179

Query: 188 LNLVYQ 194
            +L+ +
Sbjct: 190 KHLIIE 179

[Close]

BLAST of Lsi02G003640 vs. NCBI nr
Match: XP_022954432.1 (transcription factor bHLH168-like [Cucurbita moschata])

HSP 1 Score: 223.0 bits (567), Expect = 2.2e-54
Identity = 122/186 (65.59%), Postives = 150/186 (80.65%), Query Frame = 0


Query: 8   SSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYINELKER 67
           +S KLDRK VEKNRRIHMKSLCSKLFNLVP SH+KISK+LLSQQ QISYVIAYI ELKER
Sbjct: 10  ASIKLDRKIVEKNRRIHMKSLCSKLFNLVPSSHYKISKDLLSQQTQISYVIAYIKELKER 69

Query: 68  VENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDIVHIML 127
           VE+LEKR++ALQL+ HS                 L  + D T P+V++R+L + IVH+ L
Sbjct: 70  VESLEKRKEALQLQIHS----------------QLPTSSDSTFPIVEVRELCSGIVHVTL 129

Query: 128 ISSLNRSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETSRVQQRL 187
           ISS++R+ ML++II VVEEEGG+VV+AS ST+G+KVFHSLHIEAKISRVGIETSRV++RL
Sbjct: 130 ISSVDRNVMLNEIIGVVEEEGGEVVSASFSTLGDKVFHSLHIEAKISRVGIETSRVEKRL 179

Query: 188 LNLVYQ 194
            +L+ +
Sbjct: 190 KHLIIE 179

[Close]

BLAST of Lsi02G003640 vs. TAIR 10
Match: AT4G20970.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.7e-15
Identity = 66/196 (33.67%), Postives = 106/196 (54.08%), Query Frame = 0


Query: 3   NGETSSSSKLDRKTVEKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 62
           +  T  S  +DRKTVEKNRR+ MKSL S+L +L+P      S E L+  +Q+     YI 
Sbjct: 5   HSNTGQSRSVDRKTVEKNRRMQMKSLYSELISLLP---HHSSTEPLTLPDQLDEAANYIK 64

Query: 63  ELKERVENLEKRRQ------ALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLR 122
           +L+  VE   +R++       L+  N   +    +++D VS  R         LP ++++
Sbjct: 65  KLQVNVEKKRERKRNLVATTTLEKLNSVGSSSVSSSVD-VSVPR--------KLPKIEIQ 124

Query: 123 DLGNDIVHIMLISSLNRSFMLHQIISVVEEE-GGQVVNASLSTVGNKVFHSLHIEAKISR 182
           + G+ I HI L++SL   FM  +II V+ EE G ++ +A  S V + VFH+LH + +   
Sbjct: 125 ETGS-IFHIFLVTSLEHKFMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEEHD 184

Query: 183 VGIETSRVQQRLLNLV 192
            G   S++ +RL  +V
Sbjct: 185 YGAR-SQIPERLEKIV 186

[Close]

BLAST of Lsi02G003640 vs. TAIR 10
Match: AT1G10586.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.0e-13
Identity = 60/193 (31.09%), Postives = 108/193 (55.96%), Query Frame = 0


Query: 4   GETSSSSKLDRKTV-EKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 63
           GE S+SS  +++ + EK RR+ MK L S L + V P+      +L+ Q       ++Y+ 
Sbjct: 8   GEGSASSLREQRNLREKERRMRMKHLFSILSSHVSPTRRLPVPQLIDQ------AVSYMI 67

Query: 64  ELKERVENL-EKRRQAL--QLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLG 123
           +LKE+V  L E +R+ L  +++N S                         LP + +R L 
Sbjct: 68  QLKEKVNYLNEMKRRMLGGEVKNRSEGSS--------------------LLPKLSIRSL- 127

Query: 124 NDIVHIMLISSLN-RSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGI 183
           + I+ + L+  LN +  MLH+++SV EEEG QV++A+L  + ++ F+++  +A I R+GI
Sbjct: 128 DSIIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGI 173

Query: 184 ETSRVQQRLLNLV 192
           + SR+++RL +++
Sbjct: 188 DPSRIEERLRDII 173

[Close]

BLAST of Lsi02G003640 vs. TAIR 10
Match: AT1G10585.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 57/191 (29.84%), Postives = 104/191 (54.45%), Query Frame = 0


Query: 4   GETSSSSKLDRKTV-EKNRRIHMKSLCSKLFNLVPPSHFKISKELLSQQNQISYVIAYIN 63
           GE +SSS  +++ + EK+RR+ MK L S L + V P+        L   + I    +Y+ 
Sbjct: 8   GEGNSSSLREQRNLREKDRRMRMKHLFSILSSHVSPT------RKLPVPHLIDQATSYMI 67

Query: 64  ELKERVENLEKRRQALQLRNHSHNHDYQANIDRVSATRMLANNDDFTLPVVDLRDLGNDI 123
           +LKE V  L+++++ L L+    N                     F LP + +R   +  
Sbjct: 68  QLKENVNYLKEKKRTL-LQGELGN----------------LYEGSFLLPKLSIRS-RDST 127

Query: 124 VHIMLISSLN-RSFMLHQIISVVEEEGGQVVNASLSTVGNKVFHSLHIEAKISRVGIETS 183
           + + LI  LN +  MLH+++S+ EEEG QV++A+L  + ++  +++  +A ISR+GI+ S
Sbjct: 128 IEMNLIMDLNMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPS 174

Query: 184 RVQQRLLNLVY 193
           R+++R+  ++Y
Sbjct: 188 RIEERVRKIIY 174

[Close]

BLAST of Lsi02G003640 vs. TAIR 10
Match: AT1G10586.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 4.7e-10
Identity = 45/149 (30.20%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 47  LLSQQNQISYVIAYINELKERVENL-EKRRQAL--QLRNHSHNHDYQANIDRVSATRMLA 106
           LL     I   ++Y+ +LKE+V  L E +R+ L  +++N S                   
Sbjct: 5   LLPVPQLIDQAVSYMIQLKEKVNYLNEMKRRMLGGEVKNRSEGSS--------------- 64

Query: 107 NNDDFTLPVVDLRDLGNDIVHIMLISSLN-RSFMLHQIISVVEEEGGQVVNASLSTVGNK 166
                 LP + +R L + I+ + L+  LN +  MLH+++SV EEEG QV++A+L  + ++
Sbjct: 65  -----LLPKLSIRSL-DSIIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDR 124

Query: 167 VFHSLHIEAKISRVGIETSRVQQRLLNLV 192
            F+++  +A I R+GI+ SR+++RL +++
Sbjct: 125 TFYTIIAQAIICRIGIDPSRIEERLRDII 132

The following BLAST results are available for this feature:

BLAST of Lsi02G003640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JIJ7	2.3e-14	33.67	Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=...	[more]
Q9XIJ1	2.9e-12	31.09	Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=...	[more]
F4I4E1	2.4e-11	29.84	Transcription factor bHLH167 OS=Arabidopsis thaliana OX=3702 GN=BHLH167 PE=2 SV=...	[more]
Q0JFZ0	2.6e-05	26.40	Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. japonica OX=3...	[more]
A2WZ60	3.4e-05	25.51	Protein IRON-RELATED TRANSCRIPTION FACTOR 2 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of Lsi02G003640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AW43	1.4e-70	77.08	uncharacterized protein LOC103483440 OS=Cucumis melo OX=3656 GN=LOC103483440 PE=...	[more]
A0A6J1JY79	2.2e-55	64.52	transcription factor bHLH168-like OS=Cucurbita maxima OX=3661 GN=LOC111489950 PE...	[more]
A0A6J1GQZ4	1.1e-54	65.59	transcription factor bHLH168-like OS=Cucurbita moschata OX=3662 GN=LOC111456691 ...	[more]
A0A6J1C669	1.3e-44	63.19	transcription factor bHLH162-like OS=Momordica charantia OX=3673 GN=LOC111008798...	[more]
A0A6P3Z232	6.2e-42	51.58	transcription factor bHLH162-like OS=Ziziphus jujuba OX=326968 GN=LOC107406380 P...	[more]

BLAST of Lsi02G003640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008438283.1	2.9e-70	77.08	PREDICTED: uncharacterized protein LOC103483440 [Cucumis melo]	[more]
XP_031737508.1	7.7e-63	61.70	transcription factor bHLH162-like [Cucumis sativus]	[more]
XP_022994121.1	4.5e-55	64.52	transcription factor bHLH168-like [Cucurbita maxima]	[more]
XP_023542199.1	7.7e-55	65.59	transcription factor bHLH168-like [Cucurbita pepo subsp. pepo]	[more]
XP_022954432.1	2.2e-54	65.59	transcription factor bHLH168-like [Cucurbita moschata]	[more]

BLAST of Lsi02G003640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20970.1	1.7e-15	33.67	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10586.1	2.0e-13	31.09	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10585.1	1.7e-12	29.84	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10586.2	4.7e-10	30.20	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 61..88
None	No IPR available	PANTHER	PTHR13935:SF52	OS01G0108400 PROTEIN	coord: 9..190
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 9..94 e-value: 8.8E-9 score: 37.4
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 13..84
IPR015660	Achaete-scute transcription factor-related	PANTHER	PTHR13935	ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED	coord: 9..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G003640.1	Lsi02G003640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:2000112	regulation of cellular macromolecule biosynthetic process
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0090575	RNA polymerase II transcription regulator complex
molecular_function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0000977	RNA polymerase II transcription regulatory region sequence-specific DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity