Lsi02G002430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G002430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	lipid transfer-like protein VAS
Location	chr02: 2055911 .. 2057547 (-)
RNA-Seq Expression	Lsi02G002430
Synteny	Lsi02G002430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAACATATAAGGAAGGATTTCACAATCGGAATCCTTCATTTTCATCTTCATCTTCTCGATTGAAGAATTCAGATCTTCATAGTGCCGGAAACAATGGCGTCACAAAGATTCTTCGCGTTCCTCCTCTTGATTTCTTCTTGGACTTTCCTCGGATTTTCTCAAGATCTCGACGATTTTTTCAAAGGAATAGACTCGGACCTAATTCCGCAGTGTGTGCAAAAGATTCTTCCGTGTCAACCGTTCATCAAGTTGCCGGAAAATCCGTCGCCGATGTGCTGTTTACCGTTGAAGGAGATGCTGGCCGGCGATCTTCAGTGCCTCTGCTCCTTCTTCAACAATGTGGAACTGTTGAAGGTTCTGAATGTTACGCAGAGCCAAGCCTTGCAGCTTCCTAAGGCTTGCGGTGCCGCCGACGTTGATATTTCTGCCTGCAACCATGGTGAACTTCACCGAATTCCATTTCCATTTTTTATTTATGCTATTAAAATAATTTGAGCAGTATACGACGACGTTTAAGTGACCTTCGTGGTTCGTAATTCTGTTTTTCCCCCAAATTTTCAGCCGGGCCGCCGCCAACCGGTTCAACGGATGCGCCTCCGCCTCCTCCAGCCCCTAAAACTTCCGGTGGTAAATTTCTGTGATTTTTTTTAAAATTATTTTATAACTCTCTTAGTTGCCCAAAACTTCACTATTAATTGTGTGTGTGCTTTGTTGTTCATTCTTGCATAAATGATTAATTAATTTATGAATAATTTTGAGTGTTAATTTTGTATAAATTATTGGTTAATTGTGACTAAGATTTTAATTAAAATTTGATGAGTGTCAATTTCATTAAATTTTAAGCTTCAATTAATTGATTCACTATTGTTCTACTTTATTATTTTATTTCGTCCAATTTAACATTTTATTGTGTGTTTTTAAAATGTTAAAATCACTTCATCATTTTTAAAATCACTTAAAATTAATTTAATTTTCAATTATTTCAGATTTTAAAAATGACAAAAGTAATTTTAACCCAATATACCCTAAAAAGTATCTAATATAGATCATTTAGTGAATAATTGGTTTTTGGCCAATTCAATATTTTAAAAAAAATAATTATGGATTGACTTGTGAAAATTGATAGATCTATTGTATATTTTAATTTTTTAAAGACCTATTATACATCTTTTAAAAGCTTAAATTCTTAAATTCTTAGTTAAGACCTAAAAAAAATAAATTACTTTCTTAATTTTTAAAATTTCCATTATTTTCTAAAAATTGACTTGTGAAAATTGATAGATCTATTGTATATTTTAATTTTTTAAAGACCTATTATACATCTTTTAAAAGCTTAAATTCTTAAATTCTTAGTTAAGACCGAAAAAAAAATAAATTACTTTCTTAATTTTTAAAATTTATATTATTTTCTAAAAACAAATGTGGATAAAAATAATAAAGAAAGCCGTGTAAGTTTAGTTTTCAAAAATTAAAAATTAAACAGTCACGAAATAATTATTCTAAATGTTTTAAATTGAATTCAGGTTCTTCTCCGAGCAAACCTAACAGCTCATGCAGGAAAAATCCAACTTATGGGATTAGCTATGGTTTCGCAGCTGCTTCATCGTTTGTGGCTTTGTTGCTTTCAACATTATAG

mRNA sequence

GTAACATATAAGGAAGGATTTCACAATCGGAATCCTTCATTTTCATCTTCATCTTCTCGATTGAAGAATTCAGATCTTCATAGTGCCGGAAACAATGGCGTCACAAAGATTCTTCGCGTTCCTCCTCTTGATTTCTTCTTGGACTTTCCTCGGATTTTCTCAAGATCTCGACGATTTTTTCAAAGGAATAGACTCGGACCTAATTCCGCAGTGTGTGCAAAAGATTCTTCCGTGTCAACCGTTCATCAAGTTGCCGGAAAATCCGTCGCCGATGTGCTGTTTACCGTTGAAGGAGATGCTGGCCGGCGATCTTCAGTGCCTCTGCTCCTTCTTCAACAATGTGGAACTGTTGAAGGTTCTGAATGTTACGCAGAGCCAAGCCTTGCAGCTTCCTAAGGCTTGCGGTGCCGCCGACGTTGATATTTCTGCCTGCAACCATGCCGGGCCGCCGCCAACCGGTTCAACGGATGCGCCTCCGCCTCCTCCAGCCCCTAAAACTTCCGGTGGTTCTTCTCCGAGCAAACCTAACAGCTCATGCAGGAAAAATCCAACTTATGGGATTAGCTATGGTTTCGCAGCTGCTTCATCGTTTGTGGCTTTGTTGCTTTCAACATTATAG

Coding sequence (CDS)

ATGGCGTCACAAAGATTCTTCGCGTTCCTCCTCTTGATTTCTTCTTGGACTTTCCTCGGATTTTCTCAAGATCTCGACGATTTTTTCAAAGGAATAGACTCGGACCTAATTCCGCAGTGTGTGCAAAAGATTCTTCCGTGTCAACCGTTCATCAAGTTGCCGGAAAATCCGTCGCCGATGTGCTGTTTACCGTTGAAGGAGATGCTGGCCGGCGATCTTCAGTGCCTCTGCTCCTTCTTCAACAATGTGGAACTGTTGAAGGTTCTGAATGTTACGCAGAGCCAAGCCTTGCAGCTTCCTAAGGCTTGCGGTGCCGCCGACGTTGATATTTCTGCCTGCAACCATGCCGGGCCGCCGCCAACCGGTTCAACGGATGCGCCTCCGCCTCCTCCAGCCCCTAAAACTTCCGGTGGTTCTTCTCCGAGCAAACCTAACAGCTCATGCAGGAAAAATCCAACTTATGGGATTAGCTATGGTTTCGCAGCTGCTTCATCGTTTGTGGCTTTGTTGCTTTCAACATTATAG

Protein sequence

MASQRFFAFLLLISSWTFLGFSQDLDDFFKGIDSDLIPQCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGGSSPSKPNSSCRKNPTYGISYGFAAASSFVALLLSTL

Homology

[Close]

BLAST of Lsi02G002430 vs. ExPASy Swiss-Prot
Match: Q9LE56 (Non-specific lipid transfer protein GPI-anchored 3 OS=Arabidopsis thaliana OX=3702 GN=LTPG3 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 6.3e-19
Identity = 60/147 (40.82%), Postives = 84/147 (57.14%), Query Frame = 0


Query: 31  GIDSDLIPQCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLN 90
           G D+  +P C+QK++PCQP++ L   P   CC+PL E++A D  CLC+ FNNV++LK LN
Sbjct: 33  GGDAHSLP-CIQKLMPCQPYLHLATPPPATCCMPLNEIVAKDATCLCAVFNNVDMLKSLN 92

Query: 91  VTQSQALQLPKACGAADVDISACNHAGPPPTGSTD--APPPPPAPKTSGGS--------- 150
           +T+  AL LPKACG A  D+S C  +    + ST    P  PPA  TS G+         
Sbjct: 93  LTKENALDLPKACG-AKADVSLCKTSAGTNSSSTPPATPKTPPASSTSTGTGSGSTGNAA 152

Query: 151 -SPSKPNSSCRKNPTYGISYGFAAASS 166
            S +KP SS       G+S+  A  ++
Sbjct: 153 PSTAKPTSSAPAINFGGLSFASAVVAT 177

[Close]

BLAST of Lsi02G002430 vs. ExPASy Swiss-Prot
Match: Q1PFD8 (Non-specific lipid transfer protein GPI-anchored 9 OS=Arabidopsis thaliana OX=3702 GN=LTPG9 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.1e-14
Identity = 53/132 (40.15%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 40  CVQKILPCQPFI-KLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQ 99
           C+QK+LPCQP+I  L   P P CC P+KE++  D  CLC  FNN E+LK LN+T+  AL 
Sbjct: 33  CLQKLLPCQPYIHSLNPPPPPSCCGPMKEIVEKDAPCLCIAFNNPEVLKALNLTKENALL 92

Query: 100 LPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGGSSPSKPNSSCRKNPTYGISY 159
           LPKACG  + D+S C+            P P  +P ++ G+S +   S  R         
Sbjct: 93  LPKACG-VNPDVSLCSKIA--------TPSPIASPGSTNGTSSASTISFNR--------- 145

Query: 160 GFAAASSFVALL 171
            F+  S+FVA++
Sbjct: 153 -FSFLSAFVAMI 145

[Close]

BLAST of Lsi02G002430 vs. ExPASy Swiss-Prot
Match: B3H587 (Non-specific lipid transfer protein GPI-anchored 8 OS=Arabidopsis thaliana OX=3702 GN=LTPG8 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 7.4e-12
Identity = 40/109 (36.70%), Postives = 64/109 (58.72%), Query Frame = 0


Query: 39  QCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQ 98
           +CV K++PCQP+I L   P P+CC P+K++   D+ CLC+ F + +LL+ L +T+  A++
Sbjct: 34  RCVAKLMPCQPYIHLSIPPPPLCCNPMKQIAEKDVSCLCTAFKHPDLLRFLALTKENAIK 93

Query: 99  LPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGGSSPSKPNSS 148
           +  +CG  + D S CN   P       +P   P   TSG S  +K N++
Sbjct: 94  ILDSCG-INHDPSVCNKTNP------SSPAALPEAATSGNSFSTKKNTA 135

[Close]

BLAST of Lsi02G002430 vs. ExPASy Swiss-Prot
Match: Q6NLF7 (Non-specific lipid transfer protein GPI-anchored 7 OS=Arabidopsis thaliana OX=3702 GN=LTPG7 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.2e-07
Identity = 40/119 (33.61%), Postives = 57/119 (47.90%), Query Frame = 0


Query: 39  QCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQ 98
           +CV K++PC   +     P   CC  +KE +  +L CLC+ + +  LL   NVT  +AL 
Sbjct: 28  ECVSKLVPCFNDLNTTTTPVKECCDSIKEAVEKELTCLCTIYTSPGLLAQFNVTTEKALG 87

Query: 99  LPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGGSSPSKPNSSCRKNPTYGIS 158
           L + C     D+SAC   G P   S  A  PPPAP      +  K   +  K   YG++
Sbjct: 88  LSRRCNVT-TDLSACTAKGAP---SPKASLPPPAP----AGNTKKDAGAGNKLAGYGVT 138

[Close]

BLAST of Lsi02G002430 vs. ExPASy Swiss-Prot
Match: Q7EB72 (Non-specific lipid transfer protein GPI-anchored 15 OS=Arabidopsis thaliana OX=3702 GN=LTPG15 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 5.1e-05
Identity = 40/121 (33.06%), Postives = 53/121 (43.80%), Query Frame = 0


Query: 17  TFLGFSQDLDDFFKGIDSDLIPQCVQKILPCQPFIK-LPENPSPMCCLPLKEMLAGDLQC 76
           TF+     L    K   S      +  + PC  +I      PS  CC  L  ++    QC
Sbjct: 10  TFVALVAALWSVTKAQPSSSCVSTLTTLSPCLSYITGNSTTPSQPCCSRLDSVIKSSPQC 69

Query: 77  LCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTGSTDAPP-PPPAPK 136
           +CS  N+      LN+ ++QALQLP AC      ++ CN A    TG T  PP P P  K
Sbjct: 70  ICSAVNSPIPNIGLNINRTQALQLPNACNIQTPPLTQCNAA----TGPTAQPPAPSPTEK 126

[Close]

BLAST of Lsi02G002430 vs. ExPASy TrEMBL
Match: A0A1S3CQ75 (lipid transfer-like protein VAS OS=Cucumis melo OX=3656 GN=LOC103503471 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 2.1e-57
Identity = 129/170 (75.88%), Postives = 144/170 (84.71%), Query Frame = 0


Query: 6   FFAFLLLISSWTFLGFSQDLDDFFKGIDSDLIPQCVQKILPCQPFIKLPENPSPMCCLPL 65
           FFAFL+L+  ++ +GFSQDLD FFKG++S  IPQC+QKILPCQPFIKLPENPS MCCLPL
Sbjct: 8   FFAFLILL-MFSSVGFSQDLDAFFKGMESGKIPQCMQKILPCQPFIKLPENPSAMCCLPL 67

Query: 66  KEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTGSTD 125
           KEML GD+QCLCSFFN+V++LK LNVTQSQAL+LPKACGAADVDISAC   GPPPTGSTD
Sbjct: 68  KEMLVGDVQCLCSFFNDVDMLKALNVTQSQALKLPKACGAADVDISAC---GPPPTGSTD 127

Query: 126 APPPPPAPKTSGGSSPSKPNSSCR-KNPTYGISYGFAAASSFVALLLSTL 175
           APPPPPA KT   +  SK N S R  NP+ GI YGFAAASSFVALLLS L
Sbjct: 128 APPPPPA-KT---ADDSKANGSRRNNNPSKGIGYGFAAASSFVALLLSPL 169

[Close]

BLAST of Lsi02G002430 vs. ExPASy TrEMBL
Match: A0A5D3BJY6 (Lipid transfer-like protein VAS OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002450 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.1e-55
Identity = 123/157 (78.34%), Postives = 134/157 (85.35%), Query Frame = 0


Query: 19  LGFSQDLDDFFKGIDSDLIPQCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCS 78
           +GFSQDLD FFKG++S  IPQC+QKILPCQPFIKLPENPS MCCLPLKEML GD+QCLCS
Sbjct: 5   VGFSQDLDAFFKGMESGKIPQCMQKILPCQPFIKLPENPSAMCCLPLKEMLVGDVQCLCS 64

Query: 79  FFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGG 138
           FFN+V++LK LNVTQSQAL+LPKACGAADVDISAC   GPPPTGSTDAPPPPPA KT   
Sbjct: 65  FFNDVDMLKALNVTQSQALKLPKACGAADVDISAC---GPPPTGSTDAPPPPPA-KT--- 124

Query: 139 SSPSKPNSSCR-KNPTYGISYGFAAASSFVALLLSTL 175
           +  SK N S R  NP+ GI YGFAAASSFVALLLS L
Sbjct: 125 ADDSKANGSRRNNNPSKGIGYGFAAASSFVALLLSPL 154

[Close]

BLAST of Lsi02G002430 vs. ExPASy TrEMBL
Match: A0A0A0LQX5 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042900 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 4.6e-49
Identity = 120/174 (68.97%), Postives = 135/174 (77.59%), Query Frame = 0


Query: 6   FFAF--LLLISSWTFLGFSQDLDDFFKGID-SDLIPQCVQKILPCQPFIKLPENPSPMCC 65
           FFA   LL++SS   +GFS++LD FFKG+D +  IPQC+QKILPCQPF KLPE PS +CC
Sbjct: 8   FFAVLTLLIVSS---VGFSEELDGFFKGMDEATKIPQCMQKILPCQPFFKLPEIPSSICC 67

Query: 66  LPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTG 125
           LPLKEML GDLQC CSFFN+ E+LKVLN TQSQ L LPKACGA D DISAC++   PPTG
Sbjct: 68  LPLKEMLVGDLQCFCSFFNDSEMLKVLNATQSQVLNLPKACGADDADISACDNDAQPPTG 127

Query: 126 STDAPPPPPAPKTSGGSSPSKPNSSCR--KNPTYGISYGFAAASSFVALLLSTL 175
           STDAPP PPA KT+ G   SK NSS R   NP+  I YGFAAASSFVALLLS L
Sbjct: 128 STDAPPSPPA-KTADG---SKANSSRRNYNNPSNIIGYGFAAASSFVALLLSPL 174

[Close]

BLAST of Lsi02G002430 vs. ExPASy TrEMBL
Match: A0A6J1KJP7 (protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111495840 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 3.2e-42
Identity = 106/179 (59.22%), Postives = 128/179 (71.51%), Query Frame = 0


Query: 1   MASQRFFAFLLLISSWTFLGFSQDLDDFFKGIDSDL---IPQCVQKILPCQPFIKLPENP 60
           MAS RFFAFLLLISS  F+GFSQDL +F KG +S L   +P C++++L CQP+IK PENP
Sbjct: 1   MASSRFFAFLLLISSQMFVGFSQDLSEFLKGTESILDRQLPPCMRQMLSCQPYIKSPENP 60

Query: 61  SPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAG 120
              CC PLKE+L  DL CLCSFFNN ++L  LNV+QS+AL+LPK CG A++DIS C+HA 
Sbjct: 61  PTACCTPLKELLNSDLPCLCSFFNNEKMLNTLNVSQSRALELPKNCG-AELDISGCSHA- 120

Query: 121 PPPTGSTDAP----PPPPAPKTSGGSSPSKPNSSCRKNPTYGISYGFAAASSFVALLLS 173
             PTGST+ P     P P   TS G + S    + R NPTYGI +G AAASS VALLLS
Sbjct: 121 ESPTGSTEGPSSSTTPSPTANTS-GLNDSSTGGATRNNPTYGIGHGVAAASSVVALLLS 176

[Close]

BLAST of Lsi02G002430 vs. ExPASy TrEMBL
Match: A0A6J1E8R9 (protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111431672 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.7e-41
Identity = 105/180 (58.33%), Postives = 128/180 (71.11%), Query Frame = 0


Query: 1   MASQRFFAFLLLISSWTFLGFSQDLDDFFKGIDSDL---IPQCVQKILPCQPFIKLPENP 60
           MAS RF AFLLLISS  F+GFSQDL +F KG +S L   +P C++++L CQP+IK PENP
Sbjct: 1   MASSRFVAFLLLISSQMFVGFSQDLSEFLKGTESILDGNLPPCMRQMLSCQPYIKSPENP 60

Query: 61  SPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAG 120
            P CC PLKE+L  D+ CLCSFF+N ++LK LNV+QS+AL+LPK CG AD+DIS C+ A 
Sbjct: 61  PPACCTPLKELLDSDVPCLCSFFSNEKMLKTLNVSQSRALELPKNCG-ADLDISGCSDA- 120

Query: 121 PPPTGSTDAP-----PPPPAPKTSGGSSPSKPNSSCRKNPTYGISYGFAAASSFVALLLS 173
             PTGST+ P      P P   TS G + S    + R NPTYGI +G AAASS VALLLS
Sbjct: 121 ESPTGSTEGPSSSTTTPSPTANTS-GLNDSSTGGATRNNPTYGIGHGVAAASSVVALLLS 177

[Close]

BLAST of Lsi02G002430 vs. NCBI nr
Match: XP_038895529.1 (non-specific lipid transfer protein GPI-anchored 8-like [Benincasa hispida] >XP_038895530.1 non-specific lipid transfer protein GPI-anchored 8-like [Benincasa hispida])

HSP 1 Score: 280.8 bits (717), Expect = 8.0e-72
Identity = 149/176 (84.66%), Postives = 157/176 (89.20%), Query Frame = 0


Query: 1   MASQRFFAFLLLISSWTFLGFSQDLDDFFKGIDSDLIPQCVQKILPCQPFIKLPENPSPM 60
           MAS+RFFAFLLLISS  F GFSQ L +FFKG+DSDLIP C+QKILPCQPFIKLPENP P+
Sbjct: 1   MASRRFFAFLLLISSRIFFGFSQHLGNFFKGMDSDLIPPCMQKILPCQPFIKLPENPLPV 60

Query: 61  CCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACN-HAGPP 120
           CCLPLKEMLAGDL CLCSFFNN E+LK LNVTQSQAL+LPKACGAADVDISACN HAGPP
Sbjct: 61  CCLPLKEMLAGDLPCLCSFFNNAEMLKSLNVTQSQALKLPKACGAADVDISACNHHAGPP 120

Query: 121 PTGSTDAPPPPPAPKTSGGSSPSKPNSSCR-KNPTYGISYGFAAASSFVALLLSTL 175
            TGSTDAPP PPAPKTS GSSPSKP+SS R  NPTYGI YGFAAASSFVALLLS L
Sbjct: 121 STGSTDAPPSPPAPKTSDGSSPSKPDSSSRSNNPTYGIDYGFAAASSFVALLLSAL 176

[Close]

BLAST of Lsi02G002430 vs. NCBI nr
Match: XP_008465997.1 (PREDICTED: lipid transfer-like protein VAS [Cucumis melo])

HSP 1 Score: 231.9 bits (590), Expect = 4.3e-57
Identity = 129/170 (75.88%), Postives = 144/170 (84.71%), Query Frame = 0


Query: 6   FFAFLLLISSWTFLGFSQDLDDFFKGIDSDLIPQCVQKILPCQPFIKLPENPSPMCCLPL 65
           FFAFL+L+  ++ +GFSQDLD FFKG++S  IPQC+QKILPCQPFIKLPENPS MCCLPL
Sbjct: 8   FFAFLILL-MFSSVGFSQDLDAFFKGMESGKIPQCMQKILPCQPFIKLPENPSAMCCLPL 67

Query: 66  KEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTGSTD 125
           KEML GD+QCLCSFFN+V++LK LNVTQSQAL+LPKACGAADVDISAC   GPPPTGSTD
Sbjct: 68  KEMLVGDVQCLCSFFNDVDMLKALNVTQSQALKLPKACGAADVDISAC---GPPPTGSTD 127

Query: 126 APPPPPAPKTSGGSSPSKPNSSCR-KNPTYGISYGFAAASSFVALLLSTL 175
           APPPPPA KT   +  SK N S R  NP+ GI YGFAAASSFVALLLS L
Sbjct: 128 APPPPPA-KT---ADDSKANGSRRNNNPSKGIGYGFAAASSFVALLLSPL 169

[Close]

BLAST of Lsi02G002430 vs. NCBI nr
Match: TYJ99041.1 (lipid transfer-like protein VAS [Cucumis melo var. makuwa])

HSP 1 Score: 226.1 bits (575), Expect = 2.3e-55
Identity = 123/157 (78.34%), Postives = 134/157 (85.35%), Query Frame = 0


Query: 19  LGFSQDLDDFFKGIDSDLIPQCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCS 78
           +GFSQDLD FFKG++S  IPQC+QKILPCQPFIKLPENPS MCCLPLKEML GD+QCLCS
Sbjct: 5   VGFSQDLDAFFKGMESGKIPQCMQKILPCQPFIKLPENPSAMCCLPLKEMLVGDVQCLCS 64

Query: 79  FFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGG 138
           FFN+V++LK LNVTQSQAL+LPKACGAADVDISAC   GPPPTGSTDAPPPPPA KT   
Sbjct: 65  FFNDVDMLKALNVTQSQALKLPKACGAADVDISAC---GPPPTGSTDAPPPPPA-KT--- 124

Query: 139 SSPSKPNSSCR-KNPTYGISYGFAAASSFVALLLSTL 175
           +  SK N S R  NP+ GI YGFAAASSFVALLLS L
Sbjct: 125 ADDSKANGSRRNNNPSKGIGYGFAAASSFVALLLSPL 154

[Close]

BLAST of Lsi02G002430 vs. NCBI nr
Match: XP_011650886.1 (non-specific lipid-transfer protein-like protein At5g64080 [Cucumis sativus] >KGN64183.1 hypothetical protein Csa_013403 [Cucumis sativus])

HSP 1 Score: 204.1 bits (518), Expect = 9.5e-49
Identity = 120/174 (68.97%), Postives = 135/174 (77.59%), Query Frame = 0


Query: 6   FFAF--LLLISSWTFLGFSQDLDDFFKGID-SDLIPQCVQKILPCQPFIKLPENPSPMCC 65
           FFA   LL++SS   +GFS++LD FFKG+D +  IPQC+QKILPCQPF KLPE PS +CC
Sbjct: 8   FFAVLTLLIVSS---VGFSEELDGFFKGMDEATKIPQCMQKILPCQPFFKLPEIPSSICC 67

Query: 66  LPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAGPPPTG 125
           LPLKEML GDLQC CSFFN+ E+LKVLN TQSQ L LPKACGA D DISAC++   PPTG
Sbjct: 68  LPLKEMLVGDLQCFCSFFNDSEMLKVLNATQSQVLNLPKACGADDADISACDNDAQPPTG 127

Query: 126 STDAPPPPPAPKTSGGSSPSKPNSSCR--KNPTYGISYGFAAASSFVALLLSTL 175
           STDAPP PPA KT+ G   SK NSS R   NP+  I YGFAAASSFVALLLS L
Sbjct: 128 STDAPPSPPA-KTADG---SKANSSRRNYNNPSNIIGYGFAAASSFVALLLSPL 174

[Close]

BLAST of Lsi02G002430 vs. NCBI nr
Match: XP_023001811.1 (protein YLS3-like [Cucurbita maxima])

HSP 1 Score: 181.4 bits (459), Expect = 6.6e-42
Identity = 106/179 (59.22%), Postives = 128/179 (71.51%), Query Frame = 0


Query: 1   MASQRFFAFLLLISSWTFLGFSQDLDDFFKGIDSDL---IPQCVQKILPCQPFIKLPENP 60
           MAS RFFAFLLLISS  F+GFSQDL +F KG +S L   +P C++++L CQP+IK PENP
Sbjct: 1   MASSRFFAFLLLISSQMFVGFSQDLSEFLKGTESILDRQLPPCMRQMLSCQPYIKSPENP 60

Query: 61  SPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQLPKACGAADVDISACNHAG 120
              CC PLKE+L  DL CLCSFFNN ++L  LNV+QS+AL+LPK CG A++DIS C+HA 
Sbjct: 61  PTACCTPLKELLNSDLPCLCSFFNNEKMLNTLNVSQSRALELPKNCG-AELDISGCSHA- 120

Query: 121 PPPTGSTDAP----PPPPAPKTSGGSSPSKPNSSCRKNPTYGISYGFAAASSFVALLLS 173
             PTGST+ P     P P   TS G + S    + R NPTYGI +G AAASS VALLLS
Sbjct: 121 ESPTGSTEGPSSSTTPSPTANTS-GLNDSSTGGATRNNPTYGIGHGVAAASSVVALLLS 176

[Close]

BLAST of Lsi02G002430 vs. TAIR 10
Match: AT1G18280.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 4.5e-20
Identity = 60/147 (40.82%), Postives = 84/147 (57.14%), Query Frame = 0


Query: 31  GIDSDLIPQCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLN 90
           G D+  +P C+QK++PCQP++ L   P   CC+PL E++A D  CLC+ FNNV++LK LN
Sbjct: 33  GGDAHSLP-CIQKLMPCQPYLHLATPPPATCCMPLNEIVAKDATCLCAVFNNVDMLKSLN 92

Query: 91  VTQSQALQLPKACGAADVDISACNHAGPPPTGSTD--APPPPPAPKTSGGS--------- 150
           +T+  AL LPKACG A  D+S C  +    + ST    P  PPA  TS G+         
Sbjct: 93  LTKENALDLPKACG-AKADVSLCKTSAGTNSSSTPPATPKTPPASSTSTGTGSGSTGNAA 152

Query: 151 -SPSKPNSSCRKNPTYGISYGFAAASS 166
            S +KP SS       G+S+  A  ++
Sbjct: 153 PSTAKPTSSAPAINFGGLSFASAVVAT 177

[Close]

BLAST of Lsi02G002430 vs. TAIR 10
Match: AT1G73560.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.5e-15
Identity = 53/132 (40.15%), Postives = 74/132 (56.06%), Query Frame = 0


Query: 40  CVQKILPCQPFI-KLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQ 99
           C+QK+LPCQP+I  L   P P CC P+KE++  D  CLC  FNN E+LK LN+T+  AL 
Sbjct: 33  CLQKLLPCQPYIHSLNPPPPPSCCGPMKEIVEKDAPCLCIAFNNPEVLKALNLTKENALL 92

Query: 100 LPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGGSSPSKPNSSCRKNPTYGISY 159
           LPKACG  + D+S C+            P P  +P ++ G+S +   S  R         
Sbjct: 93  LPKACG-VNPDVSLCSKIA--------TPSPIASPGSTNGTSSASTISFNR--------- 145

Query: 160 GFAAASSFVALL 171
            F+  S+FVA++
Sbjct: 153 -FSFLSAFVAMI 145

[Close]

BLAST of Lsi02G002430 vs. TAIR 10
Match: AT1G73550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.3e-13
Identity = 40/109 (36.70%), Postives = 64/109 (58.72%), Query Frame = 0


Query: 39  QCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQ 98
           +CV K++PCQP+I L   P P+CC P+K++   D+ CLC+ F + +LL+ L +T+  A++
Sbjct: 34  RCVAKLMPCQPYIHLSIPPPPLCCNPMKQIAEKDVSCLCTAFKHPDLLRFLALTKENAIK 93

Query: 99  LPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGGSSPSKPNSS 148
           +  +CG  + D S CN   P       +P   P   TSG S  +K N++
Sbjct: 94  ILDSCG-INHDPSVCNKTNP------SSPAALPEAATSGNSFSTKKNTA 135

[Close]

BLAST of Lsi02G002430 vs. TAIR 10
Match: AT1G73550.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 4.5e-12
Identity = 38/104 (36.54%), Postives = 60/104 (57.69%), Query Frame = 0


Query: 39  QCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQ 98
           +CV K++PCQP+I L   P P+CC P+K++   D+ CLC+ F + +LL+ L +T+  A++
Sbjct: 34  RCVAKLMPCQPYIHLSIPPPPLCCNPMKQIAEKDVSCLCTAFKHPDLLRFLALTKENAIK 93

Query: 99  LPKACGAADVDISACNHAGPPPTGSTDAPPPPPAPKTSGGSSPS 143
           +  +CG  + D S CN   P       +P   P   TSG   P+
Sbjct: 94  ILDSCG-INHDPSVCNKTNP------SSPAALPEAATSGMHLPT 130

[Close]

BLAST of Lsi02G002430 vs. TAIR 10
Match: AT1G62790.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 3.5e-09
Identity = 36/95 (37.89%), Postives = 49/95 (51.58%), Query Frame = 0


Query: 39  QCVQKILPCQPFIKLPENPSPMCCLPLKEMLAGDLQCLCSFFNNVELLKVLNVTQSQALQ 98
           +CV K++PC   +     P   CC  +KE +  +L CLC+ + +  LL   NVT  +AL 
Sbjct: 28  ECVSKLVPCFNDLNTTTTPVKECCDSIKEAVEKELTCLCTIYTSPGLLAQFNVTTEKALG 87

Query: 99  LPKACGAADVDISACNHAGPPPTGSTDAPPPPPAP 134
           L + C     D+SAC   G P   S  A  PPPAP
Sbjct: 88  LSRRCNVT-TDLSACTAKGAP---SPKASLPPPAP 118

The following BLAST results are available for this feature:

BLAST of Lsi02G002430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE56	6.3e-19	40.82	Non-specific lipid transfer protein GPI-anchored 3 OS=Arabidopsis thaliana OX=37...	[more]
Q1PFD8	2.1e-14	40.15	Non-specific lipid transfer protein GPI-anchored 9 OS=Arabidopsis thaliana OX=37...	[more]
B3H587	7.4e-12	36.70	Non-specific lipid transfer protein GPI-anchored 8 OS=Arabidopsis thaliana OX=37...	[more]
Q6NLF7	3.2e-07	33.61	Non-specific lipid transfer protein GPI-anchored 7 OS=Arabidopsis thaliana OX=37...	[more]
Q7EB72	5.1e-05	33.06	Non-specific lipid transfer protein GPI-anchored 15 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Lsi02G002430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CQ75	2.1e-57	75.88	lipid transfer-like protein VAS OS=Cucumis melo OX=3656 GN=LOC103503471 PE=4 SV=...	[more]
A0A5D3BJY6	1.1e-55	78.34	Lipid transfer-like protein VAS OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A0A0LQX5	4.6e-49	68.97	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042900 PE=4 SV...	[more]
A0A6J1KJP7	3.2e-42	59.22	protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111495840 PE=4 SV=1	[more]
A0A6J1E8R9	2.7e-41	58.33	protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111431672 PE=4 SV=1	[more]

BLAST of Lsi02G002430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895529.1	8.0e-72	84.66	non-specific lipid transfer protein GPI-anchored 8-like [Benincasa hispida] >XP_...	[more]
XP_008465997.1	4.3e-57	75.88	PREDICTED: lipid transfer-like protein VAS [Cucumis melo]	[more]
TYJ99041.1	2.3e-55	78.34	lipid transfer-like protein VAS [Cucumis melo var. makuwa]	[more]
XP_011650886.1	9.5e-49	68.97	non-specific lipid-transfer protein-like protein At5g64080 [Cucumis sativus] >KG...	[more]
XP_023001811.1	6.6e-42	59.22	protein YLS3-like [Cucurbita maxima]	[more]

BLAST of Lsi02G002430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18280.1	4.5e-20	40.82	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G73560.1	1.5e-15	40.15	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G73550.1	5.3e-13	36.70	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G73550.2	4.5e-12	36.54	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G62790.2	3.5e-09	37.89	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 38..109 e-value: 1.2E-12 score: 49.5
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 38..106
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 37..113 e-value: 7.3E-16 score: 58.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 121..137
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 116..148
None	No IPR available	PANTHER	PTHR33044:SF100	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 6..171
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 44..104 e-value: 6.26639E-11 score: 53.5847
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 6..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G002430.1	Lsi02G002430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0005886	plasma membrane