Lsi01G020070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G020070
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Location	chr01: 27372100 .. 27378891 (+)
RNA-Seq Expression	Lsi01G020070
Synteny	Lsi01G020070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTTGAGCTATGCTGATCTCTTTTTGCTATTTCTATATTTGGCAATTTAATTTCCTTGTACGAATTGATTTGCCGTAAATTTCTAAACGACCAAGTAGGTTCCAAATATGATCCTTGGCACTAGAAAATTGTCTTCAACACTTGGAGCTGCTCCCTTGCAAACCCATCGAAATTTGACGATGAAATTGAGGATGAACCAACAATATACCGAACATAACGCTTCGAAATCCAATCATCAATCGCGCGTCGATGAGTTTCAAAAAGTTGTGCGCAAAGACCATACTGGTAACACCAAATTTCATTTGATTCCACGTTTTATATATATCTGTTCTATGATGTTTTGGCCTTGAAGCTGCATCCAACTGATATATTACTGTTTTTTGGGATCAATAATGTATGCTGGATGAGCACATAATTTTAGAATTGAATTTTGGTGATTTAGGTTGCATTTCAAGAAGAAATACATTGATAGGTCTGAAATTCGATGGAATGATTGAGATTGATAATATCTGCTTCACTTCAGAACTATTTTTAATAGCTTAACCAGTCAATAATTATTGTAGAGTTTTTTGTGTATCAGCCTGGGCATCAGCTATTGAACCTTTTTACTTGACACATTTTTCTATAAAAATGTAGATTTTAGGTATAAAATTTTGATGGATGACTAACTTTTGTCTTCAATTTAGATGAATGCCAAAACCTGATTTCACTAATTCTGGATGTTTTATACGGTAATCAGGAGTTGCTGGTCAAATATGAAAATTAGTAGCTTCCTAGTTGGTTCTCATCATTTGGTTCCTCTCTCCTTATCTTCTTTTCTTTTTAGGCGCTCGACAATAAACTGTCTTGATGAACATTGAAAATATCTTTCTTGGACCACAAAAACATTAACGTGCGATTGTTGAAGAAAAAATCAGTTTTTAGGCTTTAATCATTTGTTACTGTGAAACTAATATTAGAGTAATGAAAGGTGTGCGAAATCGAGAGGTTTTCTAGGAAAACCAGTGCTATGGTGATTAATGTCCCATTGTTGTTAAGGTGCTCGGATGGTATGACATTCAACAACTTAGGATAAATTTTCTTTTCCCTCTGTCACCTCCGACAATGCCGACACGTCCCCCTCTTTCATATATGGAGTTAAGATTATCAACTCAATTTCAATTTATATTATGAAAATTTTCCTTTTAAAGTGCTAAAAAATTGTGTTTTGGGTGGTGGGAGAATAAATTATCCAAAACAATCATGACATTGAATAAAAATAAAATAAATATATGTATTTATAATTATATTATTAGTAAAAAAACATACTTTTTAAAATTAAAATACTAAGTTCGGGTTGGTTCAAGTTGTGTTGAGTTATTGTAGGTGAACTCATGAATCAACCCAACCCATAAAATTTTCATTTATTTGAACCCAACCCAATCCGACGATTGGGTTGGGTTGATCGGTTTTTTCGGGTCATCGGGTTTTTTGAACACTCCTACGCACAGCGTAATTCAAAATAACCTCCAGCCCACTATCACTTTCCATTATACCCCTTCTAACATACTTATTCACAATTGGAGCCTAACGTATAACTTCTAGATGGATATACCAAATTTAAATTTCATGGTTTGGTTCATGCAAATGTAGCATGTCTGATTTTGGAAGGGATTCTTTTGCATGTTTTGATGTTGTGTTGGACTGCATATTGTTTCTTTTTTCTTTGTTGTCTATTGTTACAATAATATGTCATCCAGTGTGCTCATGCTTTGAAGGGTTACTAATTAGGTGGTTGGGAGATATGCTGGGAGCAAGGTCTAACACCATGGGACTTGGGACAACCAACGCCTGTGATTAGCCATCTCTGTGCAACAGGAGCTCTTCCAAATGGTCGTGTACTTGTGCCTGGATGTGGAAGTGTACGTTATTTGATTTATATATAATTTTATAGCTATTTTTACTATATATATAAAAAAAAAAAAATTCATCTATAAAGATTGAAGTATGAAAATCAAGCCAGAAAATGGCGTTGACCAAAAGGGCAAATAACCAAGAAACTCTGCTTTACATTACATTTAAGAATTCCGACCAAGAATCAAGATAAGAAAAATGTAGAGAGGTTGAGAAGTAGAAACGGAAGTGAGGCATCGAAACATTCTAAGGTTTCTCTTTGAGAGATTAACAAAGGAAATGAGGAATATATTTTTGAGAGATTTCATAACATGGGAAGTTTAATTATCAACCTTCTATAGTTCTTCGTCTTCCTTTTATCTTCGAACCTATCTTGAAGATAATAGATGTGTTCCCTTTGCATGTAGTAGTTAGCTAGAATCTTACGATTTTTCTTTTTATAGAAAACATCAAAGTTCGTAAATATGACAGGGGTATGATGTTGTGCAAATGGCATGCTCTGAACGCTATGTTGTTGGATTGGACATATCAGACAATGCCATTCAAAAGGCAAAAGAGGTATAAGTATTATATTTCTGTACTTTTCTTCTTTACAGAGCCAAAATATTGGGAGAACAAAGGAAGTTTTAGGCCGTTTTGGGAATTTTCCACAAGAGAACTATTTGTGAATGTAATTGTTGGAACCTTAGCAGATATTCTTCTTATTTGTTCTATTAATTATCCAAGGTTTATTTTATTCTAACTTTTATCATATTTAAATGTAAATTATAGTATATATGTTTAGATTTAGTTAGTCACTTCCCTGGTAGATTCTGCTACCTTCTTTGTCTTCGATCTTTCCCCCAAGCCTCATGTAGACATAAATATATCTGAGTCATCTATCTTCATAAACATCTAATTCAATTTAAAAGATAGTTTGTCTATGTGCACATGGTTTCAGTGGGCTTGTAAACGCTCGGTTTAACAATGCTTGACATTTTTAGAGTGGTCGTAGTTTGCCTTGCTATGAAAGACACAGGAAAGTTGTAATGCCCATCACCCTTTCGCTTTCAACTTATAAGTGGAAAAAGTTTTAGAAAAAGTGAGCCCTAGTAAGAACTTAGCATGAGGTCTAAAGCATATACTATTAATGTGAAACAGAAAGAAACAAAGGGGCTTGGATTTAGCTCTTTTACTCTAACATCATGAAACTAACCGAACATGAGAGAATTGCATTATTTCATTTAATAAACATGTATATGGGAGCTCTAGTTTAGGAAATGGTATATTATAATAAAGGACTAATAAATAAACGGCCCATAATAGTTATGACCCACAAAACTAACAATATTATACAACTTATGAATCATCAAAGGTAAATTTCATGAATAATGTATTTTTTTTAAAAAAATTACAAAATTAAAGTCTTCTGTTTGAAAAAGAAATTAAAGTAGTGTTGAAATGAGCTGCACCGTTAAGACTCGCTGTTACATTTGAAAACAATAACTTATTTCTATTTTTCTTTAATGAGCTGCAGCTATCTTCCTCATCACCAAATATGAGATACTTTACCTTCTTAAAGGAAGATTTTTTCAGTTGGAGACCTAAGGAATTATTTGATCTGATTTTTGATTATACGTAAGTTGGCTTATGAAAAAATTATTTCCTATTAGAAGATTCCAACATGACCGCTATTGGAAACCATGCAGGTTTTTTTCTGCTATTGAACCTGTCATGAGGCTAGCTTGGGGACAACAAATTCAGAGGCTATTAAAGCCAAATGGAGAGCTTATAACACTAATGTTTCCTGTAATCATCTTTTCTTGAGACAAGTATTCTCTATATTTTTTCCTATAAGTTTTTATCATTTTAACAAGCCTAGAATGCAAAATTTCACCTCCTATTTCATTAGCAGTTCCCCTCCATGAATTGTTTTTTTTCCTATGACAATTCGTAATTTTTAGCGCACTTTTATCTTCTCTATCTTTTTCACTAATTCTTTTTCTTGGGCTTCCTTGTCATTTCACTATAAAAAATAATTTTCTTTAACAAAAACTGTAAAATGTCAGCACAATCTTGTCTTTAAAAGTAGAGTCATTTGATTGATCTTGTGCTGCGTAATATGTTTACGATTCTTTCCATTATTTCATGTTTGAAATACAATTTATTGGCTGGCAGATCGACGACCATAGTGGTGGACCACCTTACAAAGTTTCAGTTTCAGAGTAATTCTTAACCTTCACTTTGAATTTTTTCCTACTTGTTTAATTGGTCCTTTCTGTTCAAATATTTATCTAGATCAACTTGTTTTCCTTTTTCTGTTCCCTATAAAAATTGTAACTCCTTTATCTTTAAAAAAAATTAAGGTCATAAATATGAGGGATTAGATTTCTTATCTTTACTTGTTTTGTACCTTCTTCTAATAATCATTAAAATTGTGATTAGCTAGTTGAGTTGGCTGAAAGAAACGTCCTTGTCCTTGTCCATGGGATGATATTGAAGATGAGTGATGTATTTTCTCTTAAAGAAGAGTCTTAAGTCCTTGTCCATGGGATGATATTGAAGATGAATGATGTATTTTCTCTTAAAGAAGAGTCTTAAGTGGGTTTGTGGTGAGTGTTAGAGTGATATATGATATAACATTAAATTTACCTTCACTCATCAGCTTAAACTTTTGGTTCAATCGGTGATTTAACATGGTAGTAGAGTAGGAGGTCTAAGAGATCTTGTGTTCAAACTCCCTGCAATGGCTCATTTTCTCCCTAATTAATATTGATTCCACTTGTTAGTCTTCTACATATTTCAAGTCCACAAGTAAGGAGAGTGTTAGAGTGTTGATATAATATTAAATTTACCTTCACCCATCAACTTAAGTTTTTTTTGTCAATCTCATTAGGTGGAAAGGATTGCCCTTTTCTTATCTCGTGTTCTGCCTTGGCAATAATCAACATCTCTACTCCTGTTAGGATCCTAGTTGGTGGAATAGTTTAAAGCCAAGTTGGACAAGTAGTGAAACCTCCTTGTCTCATTAGCGGTAGATTGGCTTTGGCTCAGCCTGTTCTATTCCCAACAGGATGCTCATTTACTATTTTCCAAACTTAGGGCATCTATTAAAGTTATTTACTCAATGGCGATATATGAGAGATTTCATTTGAATGGAGGGGTTTACTGAAGCCATTTGGTGAAATGGCATTGTACTTATCTCTCCATTCACTATCGAGTCTTGGGTTGGTATGAGAGAACTAGTTAAGACATTATATTTTCAACTAAAGGTCAGATGTTCAAATTTTAACTTTCACATGTTAAATTAAAAAAAGAATAAAGGTAATCAATCATCTTTTTTCAACATTGAGTTCTAATCTTAGGCGGACTCTAAACATGACTATAAATAAGTAAAAAAGTTTGGAAACTTTATAAGGCTATAGCAAATTTTGGTTGCCAAAATGCAACAAACACAGTGAAAATTAAGAATACCCGACTCATTTGGAAATAATTATTTTAAGCGAAGTAATACTACTTCAACATTGAGTTTTGTTATTATTTATTTATTTATTTACATTATTATTAATCGAAGCACTTTGTTGAAGTAGTAGAGACCATTAGGTAGCACATTTAATCATTTTTCTCAAGCCTTGATTTTGAAAAATACAATCTGCTGTAGATGAAGGGCATTTTCATATACCTGCTTGATATATTTTGTTTATTATTGTCGTTGTATCCTCTAGCTCTATTTGGAATTAGGGAAGAGAATATGTGTAAACCACTATTTTGTGGCCTTAGAGTATCCATATATGTGTTAGGATACTTCCTAACAAGAACAAGATCTGATATTTAGTACCTAACAATACGTTAAAGAATCTCAGTGGTTCAATTTTGTGGTCTAATGCGTCAACAACTAGAATTCGGAAGATTTTGTTGGAGAGAGATGGGATGTCTTTCAAGGATTTTATAGGAACTTCTTGCATATGTTGAGCTTTGTTGCATCAACTTGGCACGTTCTACATTTTCGTTTTGTTGTTACTCATTATCAGTGCATTTCACTTGAAGCGTGTTTTTGTAATCTTCATTTTCATTTTGGTTAGGAGGGTATCTTATCTTCCCTTCCCTTCTGTCATGTATATCCGATTCCTTTTGGAATCGTATTATCTTTTTTGTTTCCTTGAAAAAAGATTACTTTTTGTATCTTCGCATATATATGTGTATATATATCTCGTCCTTCCATAAACTTGGCTCATGATTACATCGTTTATTGATAGTTATGAGGACGTACTACATCCATTGGGCTTTAAGGCTGTACTGATCAAGGACAATGAACTCGCCATCCCACCTCGCAAGGTATATATAGCAACTTTTTCGGTACGCTACAGAAGTTTCAATTGTCTGTTAAAACTCGATTTTGTTGTCAACAATTAATTGGCAACAGCAAAGAGAGATCTGTTCTCTCCAAGAACACTCAAAATAAGAGTTCCAAACAAAATAGCAGAAGCTTACCCCCTCCTTCCTTCGAACACGCCCTTAAAAGGCTACTCCTGTACAGAAAAAAATGCTCACCCAAAAGATTATAATACACTGGTGCTCATTCGCCTCCCCTATTCTCTCTCTTTTTTCCTACGTGTTAGAACGTAGTGACTTGGGTCTATCATCAAGAAAGTCATGCAACCACTATTTGAGCTACTGTAAATTGTCAATTAGAGCATAACACAACTAGTTAAGGTATTGTGGCCTTTATCAAATTCTCGCCACTCAAAAGTAAATGGACTTTTATAACTTATTTTTTAAAAATGCAATGTTATCCATGTTGTGTAATTGCTGAATGTTCAGTATGATTGAAGTAAATTTTATGACAGGGACGAGAGATGATTGGAAGGTGGAAGAGGAGTTTAGCTCAAGCTTCACTCTAACAAGTTTATAGCCAC

mRNA sequence

ATGAGAAAATTGTCTTCAACACTTGGAGCTGCTCCCTTGCAAACCCATCGAAATTTGACGATGAAATTGAGGATGAACCAACAATATACCGAACATAACGCTTCGAAATCCAATCATCAATCGCGCGTCGATGAGTTTCAAAAAGTTGTGCGCAAAGACCATACTGGTGGTTGGGAGATATGCTGGGAGCAAGGTCTAACACCATGGGACTTGGGACAACCAACGCCTGTGATTAGCCATCTCTGTGCAACAGGAGCTCTTCCAAATGGTCGTGTACTTGTGCCTGGATGTGGAAGTGGGTATGATGTTGTGCAAATGGCATGCTCTGAACGCTATGTTGTTGGATTGGACATATCAGACAATGCCATTCAAAAGGCAAAAGAGAGCCAAAATATTGGGAGAACAAAGGAACTATCTTCCTCATCACCAAATATGAGATACTTTACCTTCTTAAAGGAAGATTTTTTCAGTTGGAGACCTAAGGAATTATTTGATCTGATTTTTGATTATACATTCCAACATGACCGCTATTGGAAACCATGCAGGTTTTTTTCTGCTATTGAACCTGTCATGAGGCTAGCTTGGGGACAACAAATTCAGAGGCTATTAAAGCCAAATGGAGAGCTTATAACACTAATGTTTCCTATCGACGACCATAGTGGTGGACCACCTTACAAAGTTTCAGTTTCAGATTATGAGGACGTACTACATCCATTGGGCTTTAAGGCTGTACTGATCAAGGACAATGAACTCGCCATCCCACCTCGCAAGGGACGAGAGATGATTGGAAGGTGGAAGAGGAGTTTAGCTCAAGCTTCACTCTAACAAGTTTATAGCCAC

Coding sequence (CDS)

ATGAGAAAATTGTCTTCAACACTTGGAGCTGCTCCCTTGCAAACCCATCGAAATTTGACGATGAAATTGAGGATGAACCAACAATATACCGAACATAACGCTTCGAAATCCAATCATCAATCGCGCGTCGATGAGTTTCAAAAAGTTGTGCGCAAAGACCATACTGGTGGTTGGGAGATATGCTGGGAGCAAGGTCTAACACCATGGGACTTGGGACAACCAACGCCTGTGATTAGCCATCTCTGTGCAACAGGAGCTCTTCCAAATGGTCGTGTACTTGTGCCTGGATGTGGAAGTGGGTATGATGTTGTGCAAATGGCATGCTCTGAACGCTATGTTGTTGGATTGGACATATCAGACAATGCCATTCAAAAGGCAAAAGAGAGCCAAAATATTGGGAGAACAAAGGAACTATCTTCCTCATCACCAAATATGAGATACTTTACCTTCTTAAAGGAAGATTTTTTCAGTTGGAGACCTAAGGAATTATTTGATCTGATTTTTGATTATACATTCCAACATGACCGCTATTGGAAACCATGCAGGTTTTTTTCTGCTATTGAACCTGTCATGAGGCTAGCTTGGGGACAACAAATTCAGAGGCTATTAAAGCCAAATGGAGAGCTTATAACACTAATGTTTCCTATCGACGACCATAGTGGTGGACCACCTTACAAAGTTTCAGTTTCAGATTATGAGGACGTACTACATCCATTGGGCTTTAAGGCTGTACTGATCAAGGACAATGAACTCGCCATCCCACCTCGCAAGGGACGAGAGATGATTGGAAGGTGGAAGAGGAGTTTAGCTCAAGCTTCACTCTAA

Protein sequence

MRKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKRSLAQASL

Homology

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BLAST of Lsi01G020070 vs. ExPASy Swiss-Prot
Match: Q6AWU6 (Probable thiol methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HOL3 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 6.3e-74
Identity = 138/237 (58.23%), Postives = 173/237 (73.00%), Query Frame = 0


Query: 33  NASKSNH-QSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGR 92
           NA K+   QS  D F +++ ++ +GGWE  WE G TPWDLG+PTPVI+HL  TG+LPNGR
Sbjct: 3   NAGKATSLQSSRDLFHRLMSENSSGGWEKSWEAGATPWDLGKPTPVIAHLVETGSLPNGR 62

Query: 93  VLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFL 152
            LVPGCG+GYDVV MA  +R+VVGLDIS  A++++        TK+  S+ PN +YF+FL
Sbjct: 63  ALVPGCGTGYDVVAMASPDRHVVGLDISKTAVERS--------TKKF-STLPNAKYFSFL 122

Query: 153 KEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELIT 212
            EDFF+W P E FDLIFDYT           FF A EP +R  W Q++++LLKP GELIT
Sbjct: 123 SEDFFTWEPAEKFDLIFDYT-----------FFCAFEPGVRPLWAQRMEKLLKPGGELIT 182

Query: 213 LMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKRS 269
           LMFPID+ SGGPPY+VSVS+YE VL PLGF+A+ I DNELA+ PRKG E +GRWK+S
Sbjct: 183 LMFPIDERSGGPPYEVSVSEYEKVLIPLGFEAISIVDNELAVGPRKGMEKLGRWKKS 219

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BLAST of Lsi01G020070 vs. ExPASy Swiss-Prot
Match: Q93V78 (Thiocyanate methyltransferase 1 OS=Brassica oleracea OX=3712 GN=TMT1 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 6.1e-69
Identity = 125/212 (58.96%), Postives = 153/212 (72.17%), Query Frame = 0


Query: 56  GGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVG 115
           GGWE CWE G+TPWD G+ TP++ HL  + +LP GR LVPGCG G+DVV MA  ER+VVG
Sbjct: 33  GGWEKCWEDGITPWDQGRATPLVVHLVDSSSLPLGRALVPGCGGGHDVVAMASPERFVVG 92

Query: 116 LDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHD 175
           LDIS++A++KA E+           SSP  +YFTF+KEDFF+WRP ELFDLIFDY     
Sbjct: 93  LDISESALEKAAETY---------GSSPKAKYFTFVKEDFFTWRPNELFDLIFDYV---- 152

Query: 176 RYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDV 235
                   F AIEP MR AW + +  LLKP+GELITLM+PI DH GGPPYKV+VS YEDV
Sbjct: 153 -------VFCAIEPEMRPAWAKSMYELLKPDGELITLMYPITDHDGGPPYKVAVSTYEDV 212

Query: 236 LHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           L P+GFKAV I++N  +I  RKG+E +GRWK+
Sbjct: 213 LVPVGFKAVSIEENPYSIATRKGKEKLGRWKK 224

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BLAST of Lsi01G020070 vs. ExPASy Swiss-Prot
Match: O80562 (Probable thiol methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HOL2 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.5e-67
Identity = 123/212 (58.02%), Postives = 150/212 (70.75%), Query Frame = 0


Query: 56  GGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVG 115
           GGW+ CWE G+TPWD G+ TP+I HL  + ALP GR LVPGCG G+DVV MA  ER+VVG
Sbjct: 34  GGWDKCWEDGVTPWDQGRATPLILHLLDSSALPLGRTLVPGCGGGHDVVAMASPERFVVG 93

Query: 116 LDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHD 175
           LDISD A+ KA E+           SSP   YF+F+KED F+WRP ELFDLIFDY     
Sbjct: 94  LDISDKALNKANETY---------GSSPKAEYFSFVKEDVFTWRPNELFDLIFDYV---- 153

Query: 176 RYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDV 235
                  FF AIEP MR AWG+ +  LLKP+GELITLM+P+ DH GG PYKV++S YEDV
Sbjct: 154 -------FFCAIEPEMRPAWGKSMHELLKPDGELITLMYPMTDHEGGAPYKVALSSYEDV 213

Query: 236 LHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           L P+GFKAV +++N  +IP RKG+E + RWK+
Sbjct: 214 LVPVGFKAVSVEENPDSIPTRKGKEKLARWKK 225

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BLAST of Lsi01G020070 vs. ExPASy Swiss-Prot
Match: Q0WP12 (Thiocyanate methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HOL1 PE=1 SV=2)

HSP 1 Score: 256.9 bits (655), Expect = 2.6e-67
Identity = 129/241 (53.53%), Postives = 163/241 (67.63%), Query Frame = 0


Query: 31  EHNASKSNHQSRVDEFQKVVRKDH----TGGWEICWEQGLTPWDLGQPTPVISHLCATGA 90
           + N+ +SN  + +   ++V    H     GGWE CWE+ +TPWD G+ TP+I HL  T +
Sbjct: 5   QQNSDQSNGGNVIPTPEEVATFLHKTVEEGGWEKCWEEEITPWDQGRATPLIVHLVDTSS 64

Query: 91  LPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMR 150
           LP GR LVPGCG G+DVV MA  ER+VVGLDIS++A+ KA E+           SSP   
Sbjct: 65  LPLGRALVPGCGGGHDVVAMASPERFVVGLDISESALAKANETY---------GSSPKAE 124

Query: 151 YFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPN 210
           YF+F+KED F+WRP ELFDLIFDY            FF AIEP MR AW + +  LLKP+
Sbjct: 125 YFSFVKEDVFTWRPTELFDLIFDYV-----------FFCAIEPEMRPAWAKSMYELLKPD 184

Query: 211 GELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWK 268
           GELITLM+PI DH GGPPYKV VS +E+VL P+GFKAV +++N  AIP RKG+E +GRWK
Sbjct: 185 GELITLMYPITDHVGGPPYKVDVSTFEEVLVPIGFKAVSVEENPHAIPTRKGKEKLGRWK 225

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BLAST of Lsi01G020070 vs. ExPASy Swiss-Prot
Match: Q93XC4 (Probable thiocyanate methyltransferase 2 OS=Brassica oleracea OX=3712 GN=TMT2 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.6e-67
Identity = 129/240 (53.75%), Postives = 164/240 (68.33%), Query Frame = 0


Query: 31  EHNASKSNHQSRV--DEFQKVVRKD-HTGGWEICWEQGLTPWDLGQPTPVISHLCATGAL 90
           + N+  SN ++ +  ++  K + K    GGWE CWE G+TPWD G+ TP++ HL  + +L
Sbjct: 5   QQNSGNSNGENIIPPEDVAKFLPKTVDEGGWEKCWEDGVTPWDQGRATPLVVHLVESSSL 64

Query: 91  PNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRY 150
           P GR LVPGCG G+DVV MA  ERYVVGLDIS++A++KA E+           SSP  +Y
Sbjct: 65  PLGRGLVPGCGGGHDVVAMASPERYVVGLDISESALEKAAETY---------GSSPKAKY 124

Query: 151 FTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNG 210
           FTF+KEDFF+WRP ELFDLIFDY             F AIEP  R AW + +  LLKP+G
Sbjct: 125 FTFVKEDFFTWRPNELFDLIFDYV-----------VFCAIEPETRPAWAKAMYELLKPDG 184

Query: 211 ELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           ELITLM+PI DH GGPPYKV+VS YEDVL P+GFKAV I++N  +I  RKG+E + RWK+
Sbjct: 185 ELITLMYPITDHDGGPPYKVAVSTYEDVLVPVGFKAVSIEENPYSIATRKGKEKLARWKK 224

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BLAST of Lsi01G020070 vs. ExPASy TrEMBL
Match: A0A0A0KYY5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G439050 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.5e-120
Identity = 222/273 (81.32%), Postives = 231/273 (84.62%), Query Frame = 0


Query: 3   KLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICW 62
           K  S L  APLQTHR LT+KLRMNQ  T+HNASK NHQSRV EFQKV+  DH+GGW+ICW
Sbjct: 7   KFCSILSRAPLQTHRALTLKLRMNQNSTQHNASKFNHQSRVHEFQKVMGTDHSGGWDICW 66

Query: 63  EQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNA 122
           EQGLTPWDLGQPTP+I HLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISD A
Sbjct: 67  EQGLTPWDLGQPTPLIGHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDKA 126

Query: 123 IQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCR 182
           IQKA          ELSSSSPNMR+ TFLKEDFFSWRPKELFDLIFDYT           
Sbjct: 127 IQKA---------IELSSSSPNMRHCTFLKEDFFSWRPKELFDLIFDYT----------- 186

Query: 183 FFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGF 242
           FF AIEPVMR AWGQQIQRLLKPNGELITLMFPI DDH GGPPYKVSVSDYEDVLHPLGF
Sbjct: 187 FFCAIEPVMRQAWGQQIQRLLKPNGELITLMFPIDDDHIGGPPYKVSVSDYEDVLHPLGF 246

Query: 243 KAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           KAVLI DNELA+PPRKGREMIGRWKRSLAQ SL
Sbjct: 247 KAVLINDNELAVPPRKGREMIGRWKRSLAQPSL 259

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BLAST of Lsi01G020070 vs. ExPASy TrEMBL
Match: A0A1S3BN67 (probable thiol methyltransferase 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491394 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 6.0e-120
Identity = 221/266 (83.08%), Postives = 230/266 (86.47%), Query Frame = 0


Query: 10  AAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPW 69
           AAPLQTHR LT+KLRMNQ  TEH ASK NHQSRV EFQKV+  DH+GGW+ICWE GLTPW
Sbjct: 15  AAPLQTHRALTLKLRMNQNSTEHKASKFNHQSRVHEFQKVMGTDHSGGWDICWEHGLTPW 74

Query: 70  DLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKES 129
           DLGQPTP+ISHLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISDNAIQKA   
Sbjct: 75  DLGQPTPLISHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDNAIQKA--- 134

Query: 130 QNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEP 189
                  ELSSSSPNMR+ TFL+EDFFSWRPKELFDLIFDYT           FF AIEP
Sbjct: 135 ------IELSSSSPNMRHCTFLREDFFSWRPKELFDLIFDYT-----------FFCAIEP 194

Query: 190 VMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 249
           VMR  WGQQIQRLLKPNGELITLMFPI DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD
Sbjct: 195 VMRQVWGQQIQRLLKPNGELITLMFPIDDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 254

Query: 250 NELAIPPRKGREMIGRWKRSLAQASL 275
           NELAIPPRKGREMIGRWKRSL+Q SL
Sbjct: 255 NELAIPPRKGREMIGRWKRSLSQPSL 260

[Close]

BLAST of Lsi01G020070 vs. ExPASy TrEMBL
Match: A0A6J1EBC7 (probable thiol methyltransferase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432573 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.8e-119
Identity = 219/274 (79.93%), Postives = 231/274 (84.31%), Query Frame = 0


Query: 1   MRKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEI 60
           +RKL S LGAAPLQ+HR L  KLRM Q   EHNASKSNHQSRVD+F KV+R DHTGGWE 
Sbjct: 5   IRKLPSILGAAPLQSHRRLPTKLRMGQHNAEHNASKSNHQSRVDKFSKVIRDDHTGGWET 64

Query: 61  CWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISD 120
           CWEQGLTPWDLGQPTPVISHLCA G LPNGR LVPGCGSGYDVVQMA SERYV+GLDIS+
Sbjct: 65  CWEQGLTPWDLGQPTPVISHLCAMGDLPNGRALVPGCGSGYDVVQMASSERYVIGLDISN 124

Query: 121 NAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKP 180
           NAIQKA         +ELSSSSP +RYF+FLKEDFFSWRPKELFDLIFDYT         
Sbjct: 125 NAIQKA---------RELSSSSPKIRYFSFLKEDFFSWRPKELFDLIFDYT--------- 184

Query: 181 CRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLG 240
             FFSAIEP MR+AWGQQ+Q LLKPNGELITLMFPID+H GGPPYKVSVSDYEDVL PLG
Sbjct: 185 --FFSAIEPAMRVAWGQQMQMLLKPNGELITLMFPIDNHIGGPPYKVSVSDYEDVLLPLG 244

Query: 241 FKAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           FKAVLIKDNE AIPPRKGREMIGRWKRSLA ASL
Sbjct: 245 FKAVLIKDNEHAIPPRKGREMIGRWKRSLAHASL 258

[Close]

BLAST of Lsi01G020070 vs. ExPASy TrEMBL
Match: A0A6J1D858 (probable thiol methyltransferase 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017800 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 6.7e-119
Identity = 215/273 (78.75%), Postives = 234/273 (85.71%), Query Frame = 0


Query: 2   RKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEIC 61
           RKL S LGAAP Q+HR LT K+RMN+   +HNASKS+HQSR+D+FQ V+R D TGGWEIC
Sbjct: 6   RKLPSILGAAPFQSHRVLTTKIRMNKDNAQHNASKSDHQSRLDKFQNVMRTDQTGGWEIC 65

Query: 62  WEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDN 121
           WEQGLTPWDLGQPTPVISHLCA GALPNGR LVPGCGSG+DVVQ+ACSERYV+GLDIS+N
Sbjct: 66  WEQGLTPWDLGQPTPVISHLCAIGALPNGRALVPGCGSGHDVVQLACSERYVIGLDISEN 125

Query: 122 AIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPC 181
           AIQKA         +ELSSSSPNMRYFTFLK DFFSWRPKELFDLIFDYT          
Sbjct: 126 AIQKA---------RELSSSSPNMRYFTFLKADFFSWRPKELFDLIFDYT---------- 185

Query: 182 RFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGF 241
            FFSAIEP +R AWGQQ+Q+LL+PNGELITLMFPID+H GGPPYKVSVSDYEDVL PLGF
Sbjct: 186 -FFSAIEPAVRPAWGQQMQKLLRPNGELITLMFPIDNHIGGPPYKVSVSDYEDVLLPLGF 245

Query: 242 KAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           KAVLI+DNELAIPPRKGREMIGRWKRSL QASL
Sbjct: 246 KAVLIEDNELAIPPRKGREMIGRWKRSLTQASL 258

[Close]

BLAST of Lsi01G020070 vs. ExPASy TrEMBL
Match: A0A6J1HRM4 (probable thiol methyltransferase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466088 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.8e-117
Identity = 216/274 (78.83%), Postives = 228/274 (83.21%), Query Frame = 0


Query: 1   MRKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEI 60
           +RKL S LGAA LQ+HR L  KLRM Q   EHNASKSNHQSRVD+F +V+R DHTGGWE 
Sbjct: 5   IRKLPSILGAASLQSHRRLRTKLRMGQHNAEHNASKSNHQSRVDKFSEVIRDDHTGGWET 64

Query: 61  CWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISD 120
           CWEQGLTPWDLGQPTPVISHLCA G LPNGR LVPGCGSGYDV+QMA SERYV+GLDIS 
Sbjct: 65  CWEQGLTPWDLGQPTPVISHLCAMGDLPNGRALVPGCGSGYDVLQMASSERYVIGLDISH 124

Query: 121 NAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKP 180
           NAIQKA         +ELSSSSP + YF+FLKEDFFSWRPKELFDLIFDYT         
Sbjct: 125 NAIQKA---------RELSSSSPKIGYFSFLKEDFFSWRPKELFDLIFDYT--------- 184

Query: 181 CRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLG 240
             FFSAIEP MR+AWGQQ+Q LLKPNGELITLMFPIDDH GGPPYKVSVSDYEDVL PLG
Sbjct: 185 --FFSAIEPAMRVAWGQQMQMLLKPNGELITLMFPIDDHIGGPPYKVSVSDYEDVLLPLG 244

Query: 241 FKAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           FKAVLIKDNE AIPPRKGREMIGRWKRSLA ASL
Sbjct: 245 FKAVLIKDNEHAIPPRKGREMIGRWKRSLAHASL 258

[Close]

BLAST of Lsi01G020070 vs. NCBI nr
Match: XP_038883535.1 (probable thiol methyltransferase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 473.8 bits (1218), Expect = 1.0e-129
Identity = 235/273 (86.08%), Postives = 243/273 (89.01%), Query Frame = 0


Query: 2   RKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEIC 61
           RKLSS LGAAPLQTHR LTMKLRMNQQ TEHNAS+SNHQSR+DEFQ+V+ KDH GGWEIC
Sbjct: 6   RKLSSRLGAAPLQTHRTLTMKLRMNQQSTEHNASESNHQSRLDEFQQVMHKDHNGGWEIC 65

Query: 62  WEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDN 121
           WEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVV+MACSERYVVGLDISDN
Sbjct: 66  WEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVRMACSERYVVGLDISDN 125

Query: 122 AIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPC 181
           AIQKA         +ELSSSSPN RYFTFLKEDFFSWRP ELFDLIFDYT          
Sbjct: 126 AIQKA---------RELSSSSPNRRYFTFLKEDFFSWRPMELFDLIFDYT---------- 185

Query: 182 RFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGF 241
            FFSA+EPVMR AWGQQIQRLLKPNGELITLMFPIDDH GGPPY+VSVSDYEDVLHPLGF
Sbjct: 186 -FFSALEPVMRQAWGQQIQRLLKPNGELITLMFPIDDHVGGPPYRVSVSDYEDVLHPLGF 245

Query: 242 KAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           KAVLIKDNELAIPPRKGREMIGRWKRSLAQ SL
Sbjct: 246 KAVLIKDNELAIPPRKGREMIGRWKRSLAQPSL 258

[Close]

BLAST of Lsi01G020070 vs. NCBI nr
Match: XP_038883534.1 (probable thiol methyltransferase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 442.6 bits (1137), Expect = 2.5e-120
Identity = 219/256 (85.55%), Postives = 227/256 (88.67%), Query Frame = 0


Query: 2   RKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEIC 61
           RKLSS LGAAPLQTHR LTMKLRMNQQ TEHNAS+SNHQSR+DEFQ+V+ KDH GGWEIC
Sbjct: 6   RKLSSRLGAAPLQTHRTLTMKLRMNQQSTEHNASESNHQSRLDEFQQVMHKDHNGGWEIC 65

Query: 62  WEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDN 121
           WEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVV+MACSERYVVGLDISDN
Sbjct: 66  WEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVRMACSERYVVGLDISDN 125

Query: 122 AIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPC 181
           AIQKA         +ELSSSSPN RYFTFLKEDFFSWRP ELFDLIFDYT          
Sbjct: 126 AIQKA---------RELSSSSPNRRYFTFLKEDFFSWRPMELFDLIFDYT---------- 185

Query: 182 RFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGF 241
            FFSA+EPVMR AWGQQIQRLLKPNGELITLMFPIDDH GGPPY+VSVSDYEDVLHPLGF
Sbjct: 186 -FFSALEPVMRQAWGQQIQRLLKPNGELITLMFPIDDHVGGPPYRVSVSDYEDVLHPLGF 241

Query: 242 KAVLIKDNELAIPPRK 258
           KAVLIKDNELAIPPRK
Sbjct: 246 KAVLIKDNELAIPPRK 241

[Close]

BLAST of Lsi01G020070 vs. NCBI nr
Match: XP_004144344.1 (probable thiol methyltransferase 2 [Cucumis sativus] >XP_031739580.1 probable thiol methyltransferase 2 [Cucumis sativus] >XP_031739581.1 probable thiol methyltransferase 2 [Cucumis sativus] >KGN54738.1 hypothetical protein Csa_012147 [Cucumis sativus])

HSP 1 Score: 441.0 bits (1133), Expect = 7.3e-120
Identity = 222/273 (81.32%), Postives = 231/273 (84.62%), Query Frame = 0


Query: 3   KLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICW 62
           K  S L  APLQTHR LT+KLRMNQ  T+HNASK NHQSRV EFQKV+  DH+GGW+ICW
Sbjct: 7   KFCSILSRAPLQTHRALTLKLRMNQNSTQHNASKFNHQSRVHEFQKVMGTDHSGGWDICW 66

Query: 63  EQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNA 122
           EQGLTPWDLGQPTP+I HLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISD A
Sbjct: 67  EQGLTPWDLGQPTPLIGHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDKA 126

Query: 123 IQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCR 182
           IQKA          ELSSSSPNMR+ TFLKEDFFSWRPKELFDLIFDYT           
Sbjct: 127 IQKA---------IELSSSSPNMRHCTFLKEDFFSWRPKELFDLIFDYT----------- 186

Query: 183 FFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGF 242
           FF AIEPVMR AWGQQIQRLLKPNGELITLMFPI DDH GGPPYKVSVSDYEDVLHPLGF
Sbjct: 187 FFCAIEPVMRQAWGQQIQRLLKPNGELITLMFPIDDDHIGGPPYKVSVSDYEDVLHPLGF 246

Query: 243 KAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           KAVLI DNELA+PPRKGREMIGRWKRSLAQ SL
Sbjct: 247 KAVLINDNELAVPPRKGREMIGRWKRSLAQPSL 259

[Close]

BLAST of Lsi01G020070 vs. NCBI nr
Match: XP_008449545.1 (PREDICTED: probable thiol methyltransferase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 440.3 bits (1131), Expect = 1.2e-119
Identity = 221/266 (83.08%), Postives = 230/266 (86.47%), Query Frame = 0


Query: 10  AAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPW 69
           AAPLQTHR LT+KLRMNQ  TEH ASK NHQSRV EFQKV+  DH+GGW+ICWE GLTPW
Sbjct: 15  AAPLQTHRALTLKLRMNQNSTEHKASKFNHQSRVHEFQKVMGTDHSGGWDICWEHGLTPW 74

Query: 70  DLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKES 129
           DLGQPTP+ISHLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISDNAIQKA   
Sbjct: 75  DLGQPTPLISHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDNAIQKA--- 134

Query: 130 QNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEP 189
                  ELSSSSPNMR+ TFL+EDFFSWRPKELFDLIFDYT           FF AIEP
Sbjct: 135 ------IELSSSSPNMRHCTFLREDFFSWRPKELFDLIFDYT-----------FFCAIEP 194

Query: 190 VMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 249
           VMR  WGQQIQRLLKPNGELITLMFPI DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD
Sbjct: 195 VMRQVWGQQIQRLLKPNGELITLMFPIDDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 254

Query: 250 NELAIPPRKGREMIGRWKRSLAQASL 275
           NELAIPPRKGREMIGRWKRSL+Q SL
Sbjct: 255 NELAIPPRKGREMIGRWKRSLSQPSL 260

[Close]

BLAST of Lsi01G020070 vs. NCBI nr
Match: XP_022925292.1 (probable thiol methyltransferase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 438.7 bits (1127), Expect = 3.6e-119
Identity = 219/274 (79.93%), Postives = 231/274 (84.31%), Query Frame = 0


Query: 1   MRKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEI 60
           +RKL S LGAAPLQ+HR L  KLRM Q   EHNASKSNHQSRVD+F KV+R DHTGGWE 
Sbjct: 5   IRKLPSILGAAPLQSHRRLPTKLRMGQHNAEHNASKSNHQSRVDKFSKVIRDDHTGGWET 64

Query: 61  CWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISD 120
           CWEQGLTPWDLGQPTPVISHLCA G LPNGR LVPGCGSGYDVVQMA SERYV+GLDIS+
Sbjct: 65  CWEQGLTPWDLGQPTPVISHLCAMGDLPNGRALVPGCGSGYDVVQMASSERYVIGLDISN 124

Query: 121 NAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKP 180
           NAIQKA         +ELSSSSP +RYF+FLKEDFFSWRPKELFDLIFDYT         
Sbjct: 125 NAIQKA---------RELSSSSPKIRYFSFLKEDFFSWRPKELFDLIFDYT--------- 184

Query: 181 CRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLG 240
             FFSAIEP MR+AWGQQ+Q LLKPNGELITLMFPID+H GGPPYKVSVSDYEDVL PLG
Sbjct: 185 --FFSAIEPAMRVAWGQQMQMLLKPNGELITLMFPIDNHIGGPPYKVSVSDYEDVLLPLG 244

Query: 241 FKAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           FKAVLIKDNE AIPPRKGREMIGRWKRSLA ASL
Sbjct: 245 FKAVLIKDNEHAIPPRKGREMIGRWKRSLAHASL 258

[Close]

BLAST of Lsi01G020070 vs. TAIR 10
Match: AT2G43940.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 4.5e-75
Identity = 138/237 (58.23%), Postives = 173/237 (73.00%), Query Frame = 0


Query: 33  NASKSNH-QSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGR 92
           NA K+   QS  D F +++ ++ +GGWE  WE G TPWDLG+PTPVI+HL  TG+LPNGR
Sbjct: 3   NAGKATSLQSSRDLFHRLMSENSSGGWEKSWEAGATPWDLGKPTPVIAHLVETGSLPNGR 62

Query: 93  VLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFL 152
            LVPGCG+GYDVV MA  +R+VVGLDIS  A++++        TK+  S+ PN +YF+FL
Sbjct: 63  ALVPGCGTGYDVVAMASPDRHVVGLDISKTAVERS--------TKKF-STLPNAKYFSFL 122

Query: 153 KEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELIT 212
            EDFF+W P E FDLIFDYT           FF A EP +R  W Q++++LLKP GELIT
Sbjct: 123 SEDFFTWEPAEKFDLIFDYT-----------FFCAFEPGVRPLWAQRMEKLLKPGGELIT 182

Query: 213 LMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKRS 269
           LMFPID+ SGGPPY+VSVS+YE VL PLGF+A+ I DNELA+ PRKG E +GRWK+S
Sbjct: 183 LMFPIDERSGGPPYEVSVSEYEKVLIPLGFEAISIVDNELAVGPRKGMEKLGRWKKS 219

[Close]

BLAST of Lsi01G020070 vs. TAIR 10
Match: AT2G43920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.1e-68
Identity = 123/212 (58.02%), Postives = 150/212 (70.75%), Query Frame = 0


Query: 56  GGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVG 115
           GGW+ CWE G+TPWD G+ TP+I HL  + ALP GR LVPGCG G+DVV MA  ER+VVG
Sbjct: 34  GGWDKCWEDGVTPWDQGRATPLILHLLDSSALPLGRTLVPGCGGGHDVVAMASPERFVVG 93

Query: 116 LDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHD 175
           LDISD A+ KA E+           SSP   YF+F+KED F+WRP ELFDLIFDY     
Sbjct: 94  LDISDKALNKANETY---------GSSPKAEYFSFVKEDVFTWRPNELFDLIFDYV---- 153

Query: 176 RYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDV 235
                  FF AIEP MR AWG+ +  LLKP+GELITLM+P+ DH GG PYKV++S YEDV
Sbjct: 154 -------FFCAIEPEMRPAWGKSMHELLKPDGELITLMYPMTDHEGGAPYKVALSSYEDV 213

Query: 236 LHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           L P+GFKAV +++N  +IP RKG+E + RWK+
Sbjct: 214 LVPVGFKAVSVEENPDSIPTRKGKEKLARWKK 225

[Close]

BLAST of Lsi01G020070 vs. TAIR 10
Match: AT2G43910.1 (HARMLESS TO OZONE LAYER 1 )

HSP 1 Score: 256.9 bits (655), Expect = 1.8e-68
Identity = 129/241 (53.53%), Postives = 163/241 (67.63%), Query Frame = 0


Query: 31  EHNASKSNHQSRVDEFQKVVRKDH----TGGWEICWEQGLTPWDLGQPTPVISHLCATGA 90
           + N+ +SN  + +   ++V    H     GGWE CWE+ +TPWD G+ TP+I HL  T +
Sbjct: 5   QQNSDQSNGGNVIPTPEEVATFLHKTVEEGGWEKCWEEEITPWDQGRATPLIVHLVDTSS 64

Query: 91  LPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMR 150
           LP GR LVPGCG G+DVV MA  ER+VVGLDIS++A+ KA E+           SSP   
Sbjct: 65  LPLGRALVPGCGGGHDVVAMASPERFVVGLDISESALAKANETY---------GSSPKAE 124

Query: 151 YFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPN 210
           YF+F+KED F+WRP ELFDLIFDY            FF AIEP MR AW + +  LLKP+
Sbjct: 125 YFSFVKEDVFTWRPTELFDLIFDYV-----------FFCAIEPEMRPAWAKSMYELLKPD 184

Query: 211 GELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWK 268
           GELITLM+PI DH GGPPYKV VS +E+VL P+GFKAV +++N  AIP RKG+E +GRWK
Sbjct: 185 GELITLMYPITDHVGGPPYKVDVSTFEEVLVPIGFKAVSVEENPHAIPTRKGKEKLGRWK 225

[Close]

BLAST of Lsi01G020070 vs. TAIR 10
Match: AT2G43910.2 (HARMLESS TO OZONE LAYER 1 )

HSP 1 Score: 238.8 bits (608), Expect = 5.1e-63
Identity = 122/231 (52.81%), Postives = 154/231 (66.67%), Query Frame = 0


Query: 31  EHNASKSNHQSRVDEFQKVVRKDH----TGGWEICWEQGLTPWDLGQPTPVISHLCATGA 90
           + N+ +SN  + +   ++V    H     GGWE CWE+ +TPWD G+ TP+I HL  T +
Sbjct: 5   QQNSDQSNGGNVIPTPEEVATFLHKTVEEGGWEKCWEEEITPWDQGRATPLIVHLVDTSS 64

Query: 91  LPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMR 150
           LP GR LVPGCG G+DVV MA  ER+VVGLDIS++A+ KA E+           SSP   
Sbjct: 65  LPLGRALVPGCGGGHDVVAMASPERFVVGLDISESALAKANETY---------GSSPKAE 124

Query: 151 YFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPN 210
           YF+F+KED F+WRP ELFDLIFDY            FF AIEP MR AW + +  LLKP+
Sbjct: 125 YFSFVKEDVFTWRPTELFDLIFDYV-----------FFCAIEPEMRPAWAKSMYELLKPD 184

Query: 211 GELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRK 258
           GELITLM+PI DH GGPPYKV VS +E+VL P+GFKAV +++N  AIP R+
Sbjct: 185 GELITLMYPITDHVGGPPYKVDVSTFEEVLVPIGFKAVSVEENPHAIPTRQ 215

[Close]

BLAST of Lsi01G020070 vs. TAIR 10
Match: AT2G43920.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 213.8 bits (543), Expect = 1.8e-55
Identity = 103/177 (58.19%), Postives = 122/177 (68.93%), Query Frame = 0


Query: 56  GGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVG 115
           GGW+ CWE G+TPWD G+ TP+I HL  + ALP GR LVPGCG G+DVV MA  ER+VVG
Sbjct: 34  GGWDKCWEDGVTPWDQGRATPLILHLLDSSALPLGRTLVPGCGGGHDVVAMASPERFVVG 93

Query: 116 LDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHD 175
           LDISD A+ KA E+           SSP   YF+F+KED F+WRP ELFDLIFDY     
Sbjct: 94  LDISDKALNKANETY---------GSSPKAEYFSFVKEDVFTWRPNELFDLIFDYV---- 153

Query: 176 RYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDY 233
                  FF AIEP MR AWG+ +  LLKP+GELITLM+P+ DH GG PYKV++S Y
Sbjct: 154 -------FFCAIEPEMRPAWGKSMHELLKPDGELITLMYPMTDHEGGAPYKVALSSY 190

The following BLAST results are available for this feature:

BLAST of Lsi01G020070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AWU6	6.3e-74	58.23	Probable thiol methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HOL3 PE=1 ...	[more]
Q93V78	6.1e-69	58.96	Thiocyanate methyltransferase 1 OS=Brassica oleracea OX=3712 GN=TMT1 PE=1 SV=1	[more]
O80562	1.5e-67	58.02	Probable thiol methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HOL2 PE=1 ...	[more]
Q0WP12	2.6e-67	53.53	Thiocyanate methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HOL1 PE=1 SV=...	[more]
Q93XC4	2.6e-67	53.75	Probable thiocyanate methyltransferase 2 OS=Brassica oleracea OX=3712 GN=TMT2 PE...	[more]

BLAST of Lsi01G020070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYY5	3.5e-120	81.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G439050 PE=4 SV=1	[more]
A0A1S3BN67	6.0e-120	83.08	probable thiol methyltransferase 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1EBC7	1.8e-119	79.93	probable thiol methyltransferase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1D858	6.7e-119	78.75	probable thiol methyltransferase 2 isoform X2 OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1HRM4	2.8e-117	78.83	probable thiol methyltransferase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of Lsi01G020070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883535.1	1.0e-129	86.08	probable thiol methyltransferase 2 isoform X2 [Benincasa hispida]	[more]
XP_038883534.1	2.5e-120	85.55	probable thiol methyltransferase 2 isoform X1 [Benincasa hispida]	[more]
XP_004144344.1	7.3e-120	81.32	probable thiol methyltransferase 2 [Cucumis sativus] >XP_031739580.1 probable th...	[more]
XP_008449545.1	1.2e-119	83.08	PREDICTED: probable thiol methyltransferase 2 isoform X2 [Cucumis melo]	[more]
XP_022925292.1	3.6e-119	79.93	probable thiol methyltransferase 2 isoform X1 [Cucurbita moschata]	[more]

BLAST of Lsi01G020070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43940.1	4.5e-75	58.23	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT2G43920.1	1.1e-68	58.02	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT2G43910.1	1.8e-68	53.53	HARMLESS TO OZONE LAYER 1	[more]
AT2G43910.2	5.1e-63	52.81	HARMLESS TO OZONE LAYER 1	[more]
AT2G43920.2	1.8e-55	58.19	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 24..272 e-value: 2.6E-57 score: 195.6
None	No IPR available	PANTHER	PTHR32183:SF11	THIOL METHYLTRANSFERASE 2-RELATED	coord: 26..270
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 91..211 e-value: 4.28405E-9 score: 51.2767
IPR008854	TPMT family	PFAM	PF05724	TPMT	coord: 55..257 e-value: 2.7E-40 score: 138.4
IPR008854	TPMT family	PROSITE	PS51585	SAM_MT_TPMT	coord: 55..267 score: 44.327812
IPR044995	Thiocyanate methyltransferase/thiol methyltransferase	PANTHER	PTHR32183	FAMILY NOT NAMED	coord: 26..270
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 54..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G020070.1	Lsi01G020070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity