Lsi01G018820 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G018820
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPolygalacturonase inhibitor-like
Locationchr01: 21491918 .. 21492919 (+)
RNA-Seq ExpressionLsi01G018820
SyntenyLsi01G018820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATTCTTACAACCTCCTCTTTTTCTTCTTCTTCTTCTTCTTCACCATTTCATTTGGAGAACTTTGCAAACCAAATGACAAAAAGGCCCTCCTCAACATCAAAAAAGCCTTCAACAATCCCAACATTCTCACCACATGGAAACCTGAACAAGATTGTTGCTCATGGTCTTATGTTGGATGTAATGAAAACTCCCACCGGGTCACCTCCCTCATCATCCAACAAAAAAACAAACTCTTCGGTCAAATCCCACCCTTCGTCGGCGACCTCCCATTTCTTGCTACCCTCATAATCCAGAATCAACCAAAACTCACTGGCCCCATCCCACCTACCATTGCTAAACTCCACAACCTCGTACAACTTGACCTAAGTTCAAACAGTCTCTCTGGTTCAATCCCAAGCTGTCTCGGCTCCCTCTCCAATCTCGATAATCTCGACCTCTCGTCCAACCGCCTCACCGGCTCCATCCCTAGTTCCCTTGCCAACCTCCACAACCTCAACATTCTCCATTTGAACGGAAACAAATTAACCGGACCAATCCCCAATTCCTTCGGAAAGTTCAGGCCAGAATCAAGTTTTATCCCTCAACTCTACCTCTCTCATAACCAACTTTCTGGGAAAATTCCAGCTTCCTTTGGGAGAGCGAGTTTCTATTATATTGATTTTTCGTGGAACAAACTCGAGGGTGATGGATCGGTGATATTCAGAGCAAATAAGCAGTTGACAGATATTGTAATCAATTTGTCGAGGAACTTGTTGGAGTTCAACATGTCGAAGGTTGTGTTTCCTGAAGGTTTGACTTGGTTGGATGTTAGCCATAATAAGATCTTTGGGGAAATACCGGTTGACATTGTGAAGTTGCAATTATTCTACTTGAATGTGAGTTATAATGCGCTTTGTGGTCAGATTCCGGTTGGTGGGGACTGGTTGCGGAATTTTTCTGCTTCCTCCTATTTTCATAATAAATGCTTGTGTGGTAAGCCGCTTGATAGCTGCAAATGA

mRNA sequence

ATGCATTCTTACAACCTCCTCTTTTTCTTCTTCTTCTTCTTCTTCACCATTTCATTTGGAGAACTTTGCAAACCAAATGACAAAAAGGCCCTCCTCAACATCAAAAAAGCCTTCAACAATCCCAACATTCTCACCACATGGAAACCTGAACAAGATTGTTGCTCATGGTCTTATGTTGGATGTAATGAAAACTCCCACCGGGTCACCTCCCTCATCATCCAACAAAAAAACAAACTCTTCGGTCAAATCCCACCCTTCGTCGGCGACCTCCCATTTCTTGCTACCCTCATAATCCAGAATCAACCAAAACTCACTGGCCCCATCCCACCTACCATTGCTAAACTCCACAACCTCGTACAACTTGACCTAAGTTCAAACAGTCTCTCTGGTTCAATCCCAAGCTGTCTCGGCTCCCTCTCCAATCTCGATAATCTCGACCTCTCGTCCAACCGCCTCACCGGCTCCATCCCTAGTTCCCTTGCCAACCTCCACAACCTCAACATTCTCCATTTGAACGGAAACAAATTAACCGGACCAATCCCCAATTCCTTCGGAAAGTTCAGGCCAGAATCAAGTTTTATCCCTCAACTCTACCTCTCTCATAACCAACTTTCTGGGAAAATTCCAGCTTCCTTTGGGAGAGCGAGTTTCTATTATATTGATTTTTCGTGGAACAAACTCGAGGGTGATGGATCGGTGATATTCAGAGCAAATAAGCAGTTGACAGATATTGTAATCAATTTGTCGAGGAACTTGTTGGAGTTCAACATGTCGAAGGTTGTGTTTCCTGAAGGTTTGACTTGGTTGGATGTTAGCCATAATAAGATCTTTGGGGAAATACCGGTTGACATTGTGAAGTTGCAATTATTCTACTTGAATGTGAGTTATAATGCGCTTTGTGGTCAGATTCCGGTTGGTGGGGACTGGTTGCGGAATTTTTCTGCTTCCTCCTATTTTCATAATAAATGCTTGTGTGGTAAGCCGCTTGATAGCTGCAAATGA

Coding sequence (CDS)

ATGCATTCTTACAACCTCCTCTTTTTCTTCTTCTTCTTCTTCTTCACCATTTCATTTGGAGAACTTTGCAAACCAAATGACAAAAAGGCCCTCCTCAACATCAAAAAAGCCTTCAACAATCCCAACATTCTCACCACATGGAAACCTGAACAAGATTGTTGCTCATGGTCTTATGTTGGATGTAATGAAAACTCCCACCGGGTCACCTCCCTCATCATCCAACAAAAAAACAAACTCTTCGGTCAAATCCCACCCTTCGTCGGCGACCTCCCATTTCTTGCTACCCTCATAATCCAGAATCAACCAAAACTCACTGGCCCCATCCCACCTACCATTGCTAAACTCCACAACCTCGTACAACTTGACCTAAGTTCAAACAGTCTCTCTGGTTCAATCCCAAGCTGTCTCGGCTCCCTCTCCAATCTCGATAATCTCGACCTCTCGTCCAACCGCCTCACCGGCTCCATCCCTAGTTCCCTTGCCAACCTCCACAACCTCAACATTCTCCATTTGAACGGAAACAAATTAACCGGACCAATCCCCAATTCCTTCGGAAAGTTCAGGCCAGAATCAAGTTTTATCCCTCAACTCTACCTCTCTCATAACCAACTTTCTGGGAAAATTCCAGCTTCCTTTGGGAGAGCGAGTTTCTATTATATTGATTTTTCGTGGAACAAACTCGAGGGTGATGGATCGGTGATATTCAGAGCAAATAAGCAGTTGACAGATATTGTAATCAATTTGTCGAGGAACTTGTTGGAGTTCAACATGTCGAAGGTTGTGTTTCCTGAAGGTTTGACTTGGTTGGATGTTAGCCATAATAAGATCTTTGGGGAAATACCGGTTGACATTGTGAAGTTGCAATTATTCTACTTGAATGTGAGTTATAATGCGCTTTGTGGTCAGATTCCGGTTGGTGGGGACTGGTTGCGGAATTTTTCTGCTTCCTCCTATTTTCATAATAAATGCTTGTGTGGTAAGCCGCTTGATAGCTGCAAATGA

Protein sequence

MHSYNLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVGCNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK
Homology
BLAST of Lsi01G018820 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 4.2e-96
Identity = 183/316 (57.91%), Postives = 218/316 (68.99%), Query Frame = 0

Query: 18  SFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVGCNENSHRVTSLIIQQKN 77
           +  +LC P+DKK LL IKKAF +P +L +WK + DCC W  V C+  ++R+ SL I    
Sbjct: 22  ALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDSTTNRINSLTI-FAG 81

Query: 78  KLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDLSSNSLSGSIPSCLG 137
           ++ GQIP  VGDLP+L TL    QP LTGPI P IAKL  L  L LS  +LSGS+P  L 
Sbjct: 82  QVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLSWTNLSGSVPDFLS 141

Query: 138 SLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSFGKFRPESSFIPQL 197
            L NL  LDLS N LTG+IPSSL+ L NL  L L+ NKLTG IP SFG+F      +P L
Sbjct: 142 QLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPISFGQF---IGNVPDL 201

Query: 198 YLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTDIVINLSRNLLEFNM 257
           YLSHNQLSG IP SF +  F  ID S NKLEGD SVIF  NK  T  +++LSRNLLEFN+
Sbjct: 202 YLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNK--TTQIVDLSRNLLEFNL 261

Query: 258 SKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQIPVGGDWLRNFSASS 317
           SKV FP  LT LD++HNKI+G IPV+  +L   +LNVSYN LCGQIPVGG  L++F   S
Sbjct: 262 SKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVGGK-LQSFDEYS 321

Query: 318 YFHNKCLCGKPLDSCK 334
           YFHN+CLCG PL SCK
Sbjct: 322 YFHNRCLCGAPLPSCK 330

BLAST of Lsi01G018820 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.9e-89
Identity = 174/316 (55.06%), Postives = 210/316 (66.46%), Query Frame = 0

Query: 18  SFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVGCNENSHRVTSLIIQQKN 77
           S  E C P DKK LL IKKA +NP IL +W P  DCC W  V C+  +HR+ SL I    
Sbjct: 25  SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHRINSLTI-FSG 84

Query: 78  KLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDLSSNSLSGSIPSCLG 137
           +L GQIP  VGDLPFL TLI +    LTG IPP IAKL +L  + LS  +LSG +P+   
Sbjct: 85  QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFS 144

Query: 138 SLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSFGKFRPESSFIPQL 197
            L NL  LDLS N L+G IP SL+ L NL  LHL+ N LTGPIP+SFGKF   +   P L
Sbjct: 145 ELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKF---AGSTPGL 204

Query: 198 YLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTDIVINLSRNLLEFNM 257
           +LSHNQLSGKIP SF       +D S NKLEGD S+ F ANK     +++ SRNL +F++
Sbjct: 205 HLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQ--IVDFSRNLFQFDL 264

Query: 258 SKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQIPVGGDWLRNFSASS 317
           S+V FP+ LT LD+SHNKI G +P  +  L L +LNVSYN LCG+IPVGG  L++F   S
Sbjct: 265 SRVEFPKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGK-LQSFDYDS 324

Query: 318 YFHNKCLCGKPLDSCK 334
           YFHN+CLCG PL SCK
Sbjct: 325 YFHNRCLCGAPLQSCK 333

BLAST of Lsi01G018820 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 319.3 bits (817), Expect = 5.1e-86
Identity = 167/315 (53.02%), Postives = 213/315 (67.62%), Query Frame = 0

Query: 21  ELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVGCNEN--SHRVTSLIIQQKNK 80
           +LC  +DK  LL IKK+ NNP  L +W P+ DCCSW  + C +   +HRVTSLIIQ   +
Sbjct: 23  DLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDATVNHRVTSLIIQD-GE 82

Query: 81  LFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDLSSNSLSGSIPSCLGS 140
           + GQIPP VGDLP+L +LI +    LTG I PTIAKL NL  L LS  +L+G +P  L  
Sbjct: 83  ISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRLSWTNLTGPVPEFLSQ 142

Query: 141 LSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSFGKFRPESSFIPQLY 200
           L NL+ +DLS N L+GSIPSSL++L  L  L L+ NKLTGPIP SFG F   S  +P L+
Sbjct: 143 LKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPESFGTF---SGKVPSLF 202

Query: 201 LSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTDIVINLSRNLLEFNMS 260
           LSHNQLSG IP S G   FY ID S NKL+GD S++F A K  T  ++++SRN+ +F++S
Sbjct: 203 LSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKK--TTWIVDISRNMFQFDLS 262

Query: 261 KVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQIPVGGDWLRNFSASSY 320
           KV   + L  LD++HN I G IP +  K     LNVSYN LCG+IP  G++++ F + S+
Sbjct: 263 KVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIP-KGEYIQRFDSYSF 322

Query: 321 FHNKCLCGKPLDSCK 334
           FHNKCLCG PL SCK
Sbjct: 323 FHNKCLCGAPLPSCK 330

BLAST of Lsi01G018820 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.8e-82
Identity = 170/329 (51.67%), Postives = 210/329 (63.83%), Query Frame = 0

Query: 7   LFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVGCNEN-- 66
           L F F F  T    +LC  NDK  LL IKK+ NNP  L +W P+ DCCSW  + C +   
Sbjct: 9   LLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATV 68

Query: 67  SHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDLS 126
           +HRVT+L I    ++ GQIP  VGDLP+L TL+ +    LTG I PTIAKL NL  L LS
Sbjct: 69  NHRVTALTI-FSGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLS 128

Query: 127 SNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSF 186
             +L+G IP  +  L NL+ L+LS N L+GSIPSSL+ L  +  L L+ NKLTG IP SF
Sbjct: 129 WTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESF 188

Query: 187 GKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTDI 246
           G F      +P L LSHNQLSG IP S G   F  ID S NKL+GD S++F +NK  T  
Sbjct: 189 GSF---PGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNK--TTW 248

Query: 247 VINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQIP 306
            I+LSRN+ +F++SKV  P+ L  LD++HN I G IPV   +  L + NVSYN LCG IP
Sbjct: 249 SIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIP 308

Query: 307 VGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
            GG  L+ F + SYFHNKCLCG PL+ CK
Sbjct: 309 TGGK-LQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Lsi01G018820 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.1e-70
Identity = 153/329 (46.50%), Postives = 195/329 (59.27%), Query Frame = 0

Query: 5   NLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCS-WSYVGCNE 64
           +L  FF   F T+     C  NDK ALL IKKA  NP +L++W P  DCC+ W+ V C  
Sbjct: 6   HLSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC-- 65

Query: 65  NSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDL 124
            + RVT L +    ++ GQI   +GDL  L TL     P LTG IP TI KL NL  L L
Sbjct: 66  TNRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYL 125

Query: 125 SSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNS 184
              SLSG IP  +  L +L  LDLS N+ TG IP SL+ +  L  + +N NKLTG IPNS
Sbjct: 126 KHTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNS 185

Query: 185 FGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTD 244
           FG F      +P LYLS+N+LSGKIP S  +  F  +D S N  EGD  + F  NK  T 
Sbjct: 186 FGSF---VGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNK--TT 245

Query: 245 IVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQI 304
           + ++LSRN+  F++ KV F   +  LD+S N I+G+IP  + KL L + NVS N LCG+I
Sbjct: 246 VRVDLSRNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKI 305

Query: 305 PVGGDWLRNFSASSYFHNKCLCGKPLDSC 333
           P GG  L+ F  S++ HN CLCG PL +C
Sbjct: 306 PSGG-LLQTFEPSAFAHNICLCGTPLKAC 325

BLAST of Lsi01G018820 vs. ExPASy TrEMBL
Match: K9MZG2 (Polygalacturonase-inhibiting protein OS=Cucumis melo subsp. agrestis OX=217619 GN=pgip PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 1.1e-123
Identity = 229/333 (68.77%), Postives = 261/333 (78.38%), Query Frame = 0

Query: 1   MHSYNLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVG 60
           MHS+NLL    FFFFT+SF ELC PNDK+ LLNIKKAFNNP ILT+WKPE+DCC+W  V 
Sbjct: 1   MHSHNLL-LLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVE 60

Query: 61  CNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQ 120
           C+  SHR+T+L I   ++L G IPPFVGDLPFL  L+    P LTGPIPPTIAKLHNL  
Sbjct: 61  CDLKSHRITALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKY 120

Query: 121 LDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPI 180
           LDLS N LSG IPS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGPI
Sbjct: 121 LDLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPI 180

Query: 181 PNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQ 240
           P+SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K 
Sbjct: 181 PDSFGNFKGK---VPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKK- 240

Query: 241 LTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALC 300
            T  +++LSRNLLEFN+SKVVFP  LTWLD++HNKIFGEIP  +VKL+L  LNVSYNALC
Sbjct: 241 -TTEIVDLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALC 300

Query: 301 GQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           G+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 GRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Lsi01G018820 vs. ExPASy TrEMBL
Match: Q7X9Q7 (Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=2 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 5.4e-123
Identity = 228/333 (68.47%), Postives = 260/333 (78.08%), Query Frame = 0

Query: 1   MHSYNLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVG 60
           MHS+NLL    FFFFT+SF ELC PNDK+ LLNIKKAFNNP ILT+WKPE+DCC+W  V 
Sbjct: 1   MHSHNLL-LLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVE 60

Query: 61  CNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQ 120
           C+  SHR+ +L I   ++L G IPPFVGDLPFL  L+    P LTGPIPPTIAKLHNL  
Sbjct: 61  CDLKSHRIIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKY 120

Query: 121 LDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPI 180
           LDLS N LSG IPS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGPI
Sbjct: 121 LDLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPI 180

Query: 181 PNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQ 240
           P+SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K 
Sbjct: 181 PDSFGNFKGK---VPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKK- 240

Query: 241 LTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALC 300
            T  +++LSRNLLEFN+SKVVFP  LTWLD++HNKIFGEIP  +VKL+L  LNVSYNALC
Sbjct: 241 -TTEIVDLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALC 300

Query: 301 GQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           G+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 GRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Lsi01G018820 vs. ExPASy TrEMBL
Match: A0A5D3BX47 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4559G00050 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 5.4e-123
Identity = 228/333 (68.47%), Postives = 260/333 (78.08%), Query Frame = 0

Query: 1   MHSYNLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVG 60
           MHS+NLL    FFFFT+SF ELC PNDK+ LLNIKKAFNNP ILT+WKPE+DCC+W  V 
Sbjct: 1   MHSHNLL-LLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVE 60

Query: 61  CNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQ 120
           C+  SHR+ +L I   ++L G IPPFVGDLPFL  L+    P LTGPIPPTIAKLHNL  
Sbjct: 61  CDLKSHRIIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKY 120

Query: 121 LDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPI 180
           LDLS N LSG IPS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGPI
Sbjct: 121 LDLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPI 180

Query: 181 PNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQ 240
           P+SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K 
Sbjct: 181 PDSFGNFKGK---VPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKK- 240

Query: 241 LTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALC 300
            T  +++LSRNLLEFN+SKVVFP  LTWLD++HNKIFGEIP  +VKL+L  LNVSYNALC
Sbjct: 241 -TTEIVDLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALC 300

Query: 301 GQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           G+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 GRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Lsi01G018820 vs. ExPASy TrEMBL
Match: K7QJC6 (Polygalacturonase-inhibiting protein 1 OS=Cucumis sativus OX=3659 GN=PGIP1 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 7.3e-120
Identity = 224/334 (67.07%), Postives = 256/334 (76.65%), Query Frame = 0

Query: 1   MHSYN-LLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYV 60
           MHS N LL     FFFT+S+ ELC PNDKK LLNIKKAFNNP ILT+WKPE+DCC+W  V
Sbjct: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  GCNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLV 120
            C+  SHR+ +L +   +KL G IPPFVGDLPFL  L+    P L GPIPPTIAKL+NL 
Sbjct: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120

Query: 121 QLDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGP 180
            LDLS N LSG IPS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGP
Sbjct: 121 YLDLSWNGLSGPIPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180

Query: 181 IPNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANK 240
           IP SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K
Sbjct: 181 IPESFGNFKGK---VPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKK 240

Query: 241 QLTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNAL 300
             T  +++LSRNLLEFNMSKVVFP  LT+LD++HNKIFGEIP ++VKL+L   NVSYNAL
Sbjct: 241 --TTEIVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNAL 300

Query: 301 CGQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           CG+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 CGRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 328

BLAST of Lsi01G018820 vs. ExPASy TrEMBL
Match: A0A0A0KZN2 (Polygalacturonase-inhibiting protein OS=Cucumis sativus OX=3659 GN=Csa_4G154320 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 9.6e-120
Identity = 223/334 (66.77%), Postives = 256/334 (76.65%), Query Frame = 0

Query: 1   MHSYN-LLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYV 60
           MHS N LL     FFFT+S+ ELC PNDKK LLNIKKAFNNP ILT+WKPE+DCC+W  V
Sbjct: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  GCNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLV 120
            C+  SHR+ +L +   +KL G IPPFVGDLPFL  L+    P L GPIPPTIAKL+NL 
Sbjct: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120

Query: 121 QLDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGP 180
            LDLS N LSG +PS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGP
Sbjct: 121 YLDLSWNGLSGPVPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180

Query: 181 IPNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANK 240
           IP SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K
Sbjct: 181 IPESFGNFKGK---VPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKK 240

Query: 241 QLTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNAL 300
             T  +++LSRNLLEFNMSKVVFP  LT+LD++HNKIFGEIP ++VKL+L   NVSYNAL
Sbjct: 241 --TTEIVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNAL 300

Query: 301 CGQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           CG+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 CGRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 328

BLAST of Lsi01G018820 vs. NCBI nr
Match: XP_038881259.1 (polygalacturonase inhibitor-like [Benincasa hispida])

HSP 1 Score: 512.3 bits (1318), Expect = 3.1e-141
Identity = 255/333 (76.58%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 1   MHSYNLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVG 60
           MHS+N+L FFFFFFFTISF ELC PNDKKALLNIKKA NNP+ILT+WKP +DCC+W YVG
Sbjct: 1   MHSHNIL-FFFFFFFTISFAELCNPNDKKALLNIKKALNNPHILTSWKPGKDCCTWFYVG 60

Query: 61  CNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQ 120
           C+  SHR+ SL I   + L  QIP FVG LPFL TL     PKL GPIPPTIAKLHNLVQ
Sbjct: 61  CDAKSHRINSLDISLDDDLSAQIPTFVGHLPFLTTLRFYKLPKLIGPIPPTIAKLHNLVQ 120

Query: 121 LDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPI 180
           LDLSSN+LSGSIP+ LGSLSNL+ LDLSSNRLTGSIPSSLANLH LNILHLN NKLTG +
Sbjct: 121 LDLSSNALSGSIPTFLGSLSNLEYLDLSSNRLTGSIPSSLANLHRLNILHLNRNKLTGLV 180

Query: 181 PNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQ 240
           P+SFG F   +  IPQLYLSHNQLSGKIPAS GR +F +ID SWNKLEGDGSVIF   K+
Sbjct: 181 PDSFGNF---NGNIPQLYLSHNQLSGKIPASLGRVNFNFIDLSWNKLEGDGSVIFGEKKK 240

Query: 241 LTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALC 300
                INLSRNLLEFNMSKVVFP+ L +LD++HNKIFGEIPV+IVKLQLF+LNVSYNALC
Sbjct: 241 TE--TINLSRNLLEFNMSKVVFPQTLNYLDLNHNKIFGEIPVEIVKLQLFFLNVSYNALC 300

Query: 301 GQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           G+IPVGG+WL+NF ASSYFHNKCLCGKPLDSCK
Sbjct: 301 GRIPVGGEWLQNFGASSYFHNKCLCGKPLDSCK 327

BLAST of Lsi01G018820 vs. NCBI nr
Match: AFY10521.1 (polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis])

HSP 1 Score: 453.0 bits (1164), Expect = 2.3e-123
Identity = 229/333 (68.77%), Postives = 261/333 (78.38%), Query Frame = 0

Query: 1   MHSYNLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVG 60
           MHS+NLL    FFFFT+SF ELC PNDK+ LLNIKKAFNNP ILT+WKPE+DCC+W  V 
Sbjct: 1   MHSHNLL-LLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVE 60

Query: 61  CNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQ 120
           C+  SHR+T+L I   ++L G IPPFVGDLPFL  L+    P LTGPIPPTIAKLHNL  
Sbjct: 61  CDLKSHRITALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKY 120

Query: 121 LDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPI 180
           LDLS N LSG IPS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGPI
Sbjct: 121 LDLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPI 180

Query: 181 PNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQ 240
           P+SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K 
Sbjct: 181 PDSFGNFKGK---VPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKK- 240

Query: 241 LTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALC 300
            T  +++LSRNLLEFN+SKVVFP  LTWLD++HNKIFGEIP  +VKL+L  LNVSYNALC
Sbjct: 241 -TTEIVDLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALC 300

Query: 301 GQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           G+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 GRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Lsi01G018820 vs. NCBI nr
Match: XP_038880971.1 (polygalacturonase inhibitor-like [Benincasa hispida])

HSP 1 Score: 452.6 bits (1163), Expect = 2.9e-123
Identity = 234/334 (70.06%), Postives = 262/334 (78.44%), Query Frame = 0

Query: 1   MHSYN-LLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYV 60
           MHS+N LL   F FFFT SF ELC P+DKK LLNIKKAFNNP ILT+WKPE+DCC+W  V
Sbjct: 10  MHSHNLLLLLLFLFFFTCSFAELCNPDDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 69

Query: 61  GCNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLV 120
            C++ SHR+ +L I   +KL GQIPP VGDLPFL  L+    P L GPIPPTIAKLH L 
Sbjct: 70  ECDKKSHRIIALTIFADDKLKGQIPPSVGDLPFLENLMFHKLPNLVGPIPPTIAKLHKLK 129

Query: 121 QLDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGP 180
            LDLS N LSGSIPS L SLS+LD LDLS NRLTGSIPSSLANL +L  LHL+ NKLTGP
Sbjct: 130 YLDLSWNGLSGSIPSFLDSLSDLDYLDLSFNRLTGSIPSSLANLRHLGTLHLDRNKLTGP 189

Query: 181 IPNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANK 240
           IP+SFG F+ +   IP LYLSHNQLSGKIPAS G   F YID S NKLEGD SV+F A K
Sbjct: 190 IPDSFGNFKGK---IPYLYLSHNQLSGKIPASMGGVDFNYIDLSRNKLEGDASVLFGAKK 249

Query: 241 QLTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNAL 300
             T  +INLSRNLLEF+MSKVVFP  LTWLD++HNKIFGEIP+ +VKL+L  LNVSYNAL
Sbjct: 250 --TTEIINLSRNLLEFDMSKVVFPPTLTWLDLNHNKIFGEIPMGVVKLELQMLNVSYNAL 309

Query: 301 CGQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           CG+IPVGG  L++F A SYFHNKCLCGKPL SCK
Sbjct: 310 CGRIPVGGK-LQSFDAYSYFHNKCLCGKPLGSCK 337

BLAST of Lsi01G018820 vs. NCBI nr
Match: NP_001303611.1 (polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalacturonase-inhibiting protein [Cucumis melo] >KAA0053950.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa] >TYK04261.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa])

HSP 1 Score: 450.7 bits (1158), Expect = 1.1e-122
Identity = 228/333 (68.47%), Postives = 260/333 (78.08%), Query Frame = 0

Query: 1   MHSYNLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVG 60
           MHS+NLL    FFFFT+SF ELC PNDK+ LLNIKKAFNNP ILT+WKPE+DCC+W  V 
Sbjct: 1   MHSHNLL-LLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVE 60

Query: 61  CNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQ 120
           C+  SHR+ +L I   ++L G IPPFVGDLPFL  L+    P LTGPIPPTIAKLHNL  
Sbjct: 61  CDLKSHRIIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKY 120

Query: 121 LDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPI 180
           LDLS N LSG IPS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGPI
Sbjct: 121 LDLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPI 180

Query: 181 PNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQ 240
           P+SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K 
Sbjct: 181 PDSFGNFKGK---VPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKK- 240

Query: 241 LTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALC 300
            T  +++LSRNLLEFN+SKVVFP  LTWLD++HNKIFGEIP  +VKL+L  LNVSYNALC
Sbjct: 241 -TTEIVDLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALC 300

Query: 301 GQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           G+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 GRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Lsi01G018820 vs. NCBI nr
Match: NP_001295863.1 (polygalacturonase inhibitor-like precursor [Cucumis sativus] >AFU51543.1 polygalacturonase-inhibiting protein 1 [Cucumis sativus])

HSP 1 Score: 440.3 bits (1131), Expect = 1.5e-119
Identity = 224/334 (67.07%), Postives = 256/334 (76.65%), Query Frame = 0

Query: 1   MHSYN-LLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYV 60
           MHS N LL     FFFT+S+ ELC PNDKK LLNIKKAFNNP ILT+WKPE+DCC+W  V
Sbjct: 1   MHSPNLLLLLLLLFFFTVSYAELCHPNDKKVLLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  GCNENSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLV 120
            C+  SHR+ +L +   +KL G IPPFVGDLPFL  L+    P L GPIPPTIAKL+NL 
Sbjct: 61  ECDRKSHRIIALTVFADDKLSGPIPPFVGDLPFLENLMFHKLPNLIGPIPPTIAKLNNLK 120

Query: 121 QLDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGP 180
            LDLS N LSG IPS LGSLSNLD LDLS NR TGSIPSSLANL  L  LHL+ NKLTGP
Sbjct: 121 YLDLSWNGLSGPIPSFLGSLSNLDVLDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGP 180

Query: 181 IPNSFGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANK 240
           IP SFG F+ +   +P LYLSHNQLSGKIP S G+  F YID S NKL GDGS+IF + K
Sbjct: 181 IPESFGNFKGK---VPYLYLSHNQLSGKIPISMGKVDFNYIDLSRNKLVGDGSLIFGSKK 240

Query: 241 QLTDIVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNAL 300
             T  +++LSRNLLEFNMSKVVFP  LT+LD++HNKIFGEIP ++VKL+L   NVSYNAL
Sbjct: 241 --TTEIVDLSRNLLEFNMSKVVFPRTLTYLDLNHNKIFGEIPTEVVKLELQMFNVSYNAL 300

Query: 301 CGQIPVGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
           CG+IP+GG  L++F   SYFHNKCLCGKPL SCK
Sbjct: 301 CGRIPMGGK-LQSFDVYSYFHNKCLCGKPLGSCK 328

BLAST of Lsi01G018820 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 319.3 bits (817), Expect = 3.6e-87
Identity = 167/315 (53.02%), Postives = 213/315 (67.62%), Query Frame = 0

Query: 21  ELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVGCNEN--SHRVTSLIIQQKNK 80
           +LC  +DK  LL IKK+ NNP  L +W P+ DCCSW  + C +   +HRVTSLIIQ   +
Sbjct: 23  DLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDATVNHRVTSLIIQD-GE 82

Query: 81  LFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDLSSNSLSGSIPSCLGS 140
           + GQIPP VGDLP+L +LI +    LTG I PTIAKL NL  L LS  +L+G +P  L  
Sbjct: 83  ISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRLSWTNLTGPVPEFLSQ 142

Query: 141 LSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSFGKFRPESSFIPQLY 200
           L NL+ +DLS N L+GSIPSSL++L  L  L L+ NKLTGPIP SFG F   S  +P L+
Sbjct: 143 LKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPESFGTF---SGKVPSLF 202

Query: 201 LSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTDIVINLSRNLLEFNMS 260
           LSHNQLSG IP S G   FY ID S NKL+GD S++F A K  T  ++++SRN+ +F++S
Sbjct: 203 LSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKK--TTWIVDISRNMFQFDLS 262

Query: 261 KVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQIPVGGDWLRNFSASSY 320
           KV   + L  LD++HN I G IP +  K     LNVSYN LCG+IP  G++++ F + S+
Sbjct: 263 KVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIP-KGEYIQRFDSYSF 322

Query: 321 FHNKCLCGKPLDSCK 334
           FHNKCLCG PL SCK
Sbjct: 323 FHNKCLCGAPLPSCK 330

BLAST of Lsi01G018820 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 305.8 bits (782), Expect = 4.1e-83
Identity = 170/329 (51.67%), Postives = 210/329 (63.83%), Query Frame = 0

Query: 7   LFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCSWSYVGCNEN-- 66
           L F F F  T    +LC  NDK  LL IKK+ NNP  L +W P+ DCCSW  + C +   
Sbjct: 9   LLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATV 68

Query: 67  SHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDLS 126
           +HRVT+L I    ++ GQIP  VGDLP+L TL+ +    LTG I PTIAKL NL  L LS
Sbjct: 69  NHRVTALTI-FSGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLS 128

Query: 127 SNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSF 186
             +L+G IP  +  L NL+ L+LS N L+GSIPSSL+ L  +  L L+ NKLTG IP SF
Sbjct: 129 WTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESF 188

Query: 187 GKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTDI 246
           G F      +P L LSHNQLSG IP S G   F  ID S NKL+GD S++F +NK  T  
Sbjct: 189 GSF---PGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNK--TTW 248

Query: 247 VINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQIP 306
            I+LSRN+ +F++SKV  P+ L  LD++HN I G IPV   +  L + NVSYN LCG IP
Sbjct: 249 SIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIP 308

Query: 307 VGGDWLRNFSASSYFHNKCLCGKPLDSCK 334
            GG  L+ F + SYFHNKCLCG PL+ CK
Sbjct: 309 TGGK-LQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Lsi01G018820 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.6e-71
Identity = 153/329 (46.50%), Postives = 195/329 (59.27%), Query Frame = 0

Query: 5   NLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNPNILTTWKPEQDCCS-WSYVGCNE 64
           +L  FF   F T+     C  NDK ALL IKKA  NP +L++W P  DCC+ W+ V C  
Sbjct: 6   HLSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC-- 65

Query: 65  NSHRVTSLIIQQKNKLFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHNLVQLDL 124
            + RVT L +    ++ GQI   +GDL  L TL     P LTG IP TI KL NL  L L
Sbjct: 66  TNRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYL 125

Query: 125 SSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNS 184
              SLSG IP  +  L +L  LDLS N+ TG IP SL+ +  L  + +N NKLTG IPNS
Sbjct: 126 KHTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNS 185

Query: 185 FGKFRPESSFIPQLYLSHNQLSGKIPASFGRASFYYIDFSWNKLEGDGSVIFRANKQLTD 244
           FG F      +P LYLS+N+LSGKIP S  +  F  +D S N  EGD  + F  NK  T 
Sbjct: 186 FGSF---VGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNK--TT 245

Query: 245 IVINLSRNLLEFNMSKVVFPEGLTWLDVSHNKIFGEIPVDIVKLQLFYLNVSYNALCGQI 304
           + ++LSRN+  F++ KV F   +  LD+S N I+G+IP  + KL L + NVS N LCG+I
Sbjct: 246 VRVDLSRNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKI 305

Query: 305 PVGGDWLRNFSASSYFHNKCLCGKPLDSC 333
           P GG  L+ F  S++ HN CLCG PL +C
Sbjct: 306 PSGG-LLQTFEPSAFAHNICLCGTPLKAC 325

BLAST of Lsi01G018820 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 175.6 bits (444), Expect = 6.5e-44
Identity = 120/357 (33.61%), Postives = 179/357 (50.14%), Query Frame = 0

Query: 13  FFFTISFGELCKPNDKKALLNIKKAFNNPN--ILTTWKPEQDCC-SWSYVGCNENSHRVT 72
           F   IS    C P D+ AL   K + + PN  I  TW    DCC  W  + C+ +S RVT
Sbjct: 17  FISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYGISCDPDSGRVT 76

Query: 73  SLIIQQKNK------------LFGQIPPFVGDLPFLATLIIQNQPKLTGPIPPTIAKLHN 132
            + ++ +++            + G I P V DL  L +L++ +   +TG IPP I  L +
Sbjct: 77  DISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITGEIPPCITSLAS 136

Query: 133 LVQLDLSSNSLSGSIPSCLGSLSNLDNLDLSSNRLTGSIPSSLANLHNLNILHLNGNKLT 192
           L  LDL+ N ++G IP+ +G LS L  L+L+ N+++G IP+SL +L  L  L L  N +T
Sbjct: 137 LRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIELKHLELTENGIT 196

Query: 193 GPIPNSFGKFRPESSFIPQLYLSHNQLSGKIPASF-GRASFYYIDFSWNKLE-------G 252
           G IP  FG  +     + ++ L  N+L+G IP S  G      +D S N +E       G
Sbjct: 197 GVIPADFGSLK----MLSRVLLGRNELTGSIPESISGMERLADLDLSKNHIEGPIPEWMG 256

Query: 253 DGSVIFRANKQLTDI---------------VINLSRNLLEFNMSKVVFPEG-LTWLDVSH 312
           +  V+   N     +               V NLSRN LE  +  V   +  L  LD+SH
Sbjct: 257 NMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTYLVSLDLSH 316

Query: 313 NKIFGEIPVDIVKLQLF-YLNVSYNALCGQIPVGGDWLRNFSASSYFHNKCLCGKPL 330
           N + G IP  +   +   +L++S+N LCG+IP G  +  +  A+S+  N+CLCG PL
Sbjct: 317 NSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPF-DHLEATSFSDNQCLCGGPL 368

BLAST of Lsi01G018820 vs. TAIR 10
Match: AT3G20820.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-41
Identity = 120/371 (32.35%), Postives = 185/371 (49.87%), Query Frame = 0

Query: 5   NLLFFFFFFFFTISFGELCKPNDKKALLNIKKAFNNP--NILTTWKPEQDCCSWSYVGCN 64
           NL          IS    C P+D++ALL  + A + P   I  +W  +  C +W  + C+
Sbjct: 2   NLNVVVLLLLLLISSATCCPPSDRRALLAFRSALHEPYLGIFNSWTGQDCCHNWYGISCD 61

Query: 65  ENSHRVTSLIIQQKNK-------------------------------------LFGQIPP 124
             +HRV  + ++ +++                                     + G+IP 
Sbjct: 62  SLTHRVADINLRGESEDPIFERAHRTGYMTGHISASICELTRLSAITIADWKGISGEIPK 121

Query: 125 FVGDLPFLATL-IIQNQPKLTGPIPPTIAKLHNLVQLDLSSNSLSGSIPSCLGSLSNLDN 184
            +  LPFL TL +I NQ  ++G IP  I +L+ L  L+++ N +SGSIP  L +LS+L +
Sbjct: 122 CITRLPFLRTLDLIGNQ--ISGGIPYDIGRLNRLAVLNVADNRISGSIPKSLTNLSSLMH 181

Query: 185 LDLSSNRLTGSIPSSLANLHNLNILHLNGNKLTGPIPNSFGKFRPESSFIPQLYLSHNQL 244
           LDL +N ++G IPS +  L  L+   L+GN++TG IP S          +  + LS NQL
Sbjct: 182 LDLRNNLISGVIPSDVGRLKMLSRALLSGNRITGRIPESLTNIYR----LADVDLSGNQL 241

Query: 245 SGKIPASFGRAS-FYYIDFSWNKLEGDGSVIFRANKQLTDIVINLSRNLLEFNMSKVVFP 304
            G IP S GR S    ++   NK+ G+       +  +    +NLSRNLL+  + +   P
Sbjct: 242 YGTIPPSLGRMSVLATLNLDGNKISGEIPQTLMTSSVMN---LNLSRNLLQGKIPEGFGP 301

Query: 305 EG-LTWLDVSHNKIFGEIPVDIVKLQLF-YLNVSYNALCGQIPVGGDWLRNFSASSYFHN 333
               T LD+S+N + G IP  I       +L++S+N LCG+IPVG  +  +  A+S+  N
Sbjct: 302 RSYFTVLDLSYNNLKGPIPRSISGASFIGHLDLSHNHLCGRIPVGSPF-DHLEAASFMFN 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050914.2e-9657.91Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
A7PW812.9e-8955.06Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9M5J85.1e-8653.02Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
Q9M5J95.8e-8251.67Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9LH525.1e-7046.50Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
K9MZG21.1e-12368.77Polygalacturonase-inhibiting protein OS=Cucumis melo subsp. agrestis OX=217619 G... [more]
Q7X9Q75.4e-12368.47Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=... [more]
A0A5D3BX475.4e-12368.47Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
K7QJC67.3e-12067.07Polygalacturonase-inhibiting protein 1 OS=Cucumis sativus OX=3659 GN=PGIP1 PE=2 ... [more]
A0A0A0KZN29.6e-12066.77Polygalacturonase-inhibiting protein OS=Cucumis sativus OX=3659 GN=Csa_4G154320 ... [more]
Match NameE-valueIdentityDescription
XP_038881259.13.1e-14176.58polygalacturonase inhibitor-like [Benincasa hispida][more]
AFY10521.12.3e-12368.77polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis][more]
XP_038880971.12.9e-12370.06polygalacturonase inhibitor-like [Benincasa hispida][more]
NP_001303611.11.1e-12268.47polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalact... [more]
NP_001295863.11.5e-11967.07polygalacturonase inhibitor-like precursor [Cucumis sativus] >AFU51543.1 polygal... [more]
Match NameE-valueIdentityDescription
AT5G06870.13.6e-8753.02polygalacturonase inhibiting protein 2 [more]
AT5G06860.14.1e-8351.67polygalacturonase inhibiting protein 1 [more]
AT3G12145.13.6e-7146.50Leucine-rich repeat (LRR) family protein [more]
AT3G12610.16.5e-4433.61Leucine-rich repeat (LRR) family protein [more]
AT3G20820.11.8e-4132.35Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00019LEURICHRPTcoord: 118..131
score: 54.43
coord: 139..152
score: 54.43
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 7..332
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 22..330
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 93..152
e-value: 2.9E-7
score: 30.2
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 165..187
e-value: 0.19
score: 12.4
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 141..164
score: 7.319219
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 25..62
e-value: 1.1E-10
score: 41.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 21..333
e-value: 3.5E-82
score: 278.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G018820.1Lsi01G018820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding