Lsi01G010690 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G010690
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	alkane hydroxylase MAH1-like
Location	chr01: 8860066 .. 8861463 (-)
RNA-Seq Expression	Lsi01G010690
Synteny	Lsi01G010690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACTTAGTGTTATTACTCATATTGATGAATCTCATGATCGTATTACTGAAGTCTTACAAGAAGCTGGTTCAACTTTTTTCTTCAAAGGCGTTTGGTTTTCAGGTACGGACTTTCTCCTCACAGTTGACCCTTCAAATATCCACCATATTTTGAGTTCAAATTTCGAACGCTATCCAAAAGGCCCTGATTTCAAGTATCTTTTCGACGTTTTGGGAGATGGAATTTTCAACTCCGATTCAGATGTTTGGAAAAATCAACGCAAAATCGCTCATTTCTTGGTTCATGATGAAAATTTTCTTCAATTCTTGGAGAAAATTACATTGAAGAAAGTGAAGGAAGGGTTGATTCCGGTGCTTGAAAGTGTTTGTGAAAATGGGTTGGTGTTGGATTTACAAGATGTGTTTCAAAGATTCTCTTTTGATTCAACTTGTATGTTGGTCACTGGCTTCGACCTCCATAGTTTATCTCTTGAACTCCCTCAAGTTCCTTTTTCCAAGGCCATGGATGATATAGAGGAAGTGATTTTTCTCCGACATTTTTTTCCTAAAAAAATATGGGAATTTCAAAAGAAACTCCAAATTGGACAACCCAAGAGGCTGAAACAAGCTTGGGAAATCATAGATGAAACCATCGCCAAATTGATAGCCTTCAAAAGGGAGAGCTTGAAAAATCAGCTGAACGAAGAGGGAGATAAGCAAGGAGGAGTTGATTTGATCACATCATATATTACAGATACAGAAAACGATGATAAATTTTTGAGAGATACTATTTTAAATTTTATGATTGCAGGTAGAGACACTCTAAGTTCAGCTCTCTCTTGGTTTTTCTTTTGTCTTTCAAAAAATTCAATCATCGTAGAGAAAATCAGAGAAGAGCTCAAAACAGCAATTCCACCGAATGAAGCTCGAGATCAATGGCGGATATTTTCGATTGAAGAGATAGATAAGTTAGTTTACTTTCATGGAACTTTGTGTGAAGCTCTTAGATTATATCCTCCAGTCCCACTCCAACATAAGGTGTCAGTACAACATGATATCCTCCCAAGTGGTCATCACATAAATCCTAAAACAAAGATTGTGTTTTCTTTATATGCATTGGGAAGAATGAGTGATGTGTGGGGGAAAGATTGCATGGAGTTCAAGCCAGAGAGATGGATTTCAGAAAAGGGAAGAATCAAACATGTGCCATCTTACAAATTCTTGGCCTTCAATTCTGGTCCAAGAACTTGCTTAGGAAAACAAGTGGCCTTCATTGAATTGAAAATAGTTGCAGCTGCCATTATTCATAACTACAATATTATTCAACAAACAGGACATGACGTTGTTCCAAATGCTTCCATTATTCTTCATATGAAGCATGGATTCAACGCCAGGGTTACAAAGAGATGGACTTGA

mRNA sequence

ATGACACTTAGTGTTATTACTCATATTGATGAATCTCATGATCGTATTACTGAAGTCTTACAAGAAGCTGGTTCAACTTTTTTCTTCAAAGGCGTTTGGTTTTCAGGTACGGACTTTCTCCTCACAGTTGACCCTTCAAATATCCACCATATTTTGAGTTCAAATTTCGAACGCTATCCAAAAGGCCCTGATTTCAAGTATCTTTTCGACGTTTTGGGAGATGGAATTTTCAACTCCGATTCAGATGTTTGGAAAAATCAACGCAAAATCGCTCATTTCTTGGTTCATGATGAAAATTTTCTTCAATTCTTGGAGAAAATTACATTGAAGAAAGTGAAGGAAGGGTTGATTCCGGTGCTTGAAAGTGTTTGTGAAAATGGGTTGGTGTTGGATTTACAAGATGTGTTTCAAAGATTCTCTTTTGATTCAACTTGTAGAGACACTCTAAGTTCAGCTCTCTCTTGGTTTTTCTTTTGTCTTTCAAAAAATTCAATCATCGTAGAGAAAATCAGAGAAGAGCTCAAAACAGCAATTCCACCGAATGAAGCTCGAGATCAATGGCGGATATTTTCGATTGAAGAGATAGATAAGTTAGTTTACTTTCATGGAACTTTGTGTGAAGCTCTTAGATTATATCCTCCAGTCCCACTCCAACATAAGGTGTCAGTACAACATGATATCCTCCCAAGTGGTCATCACATAAATCCTAAAACAAAGATTGTGTTTTCTTTATATGCATTGGGAAGAATGAGTGATGTGTGGGGGAAAGATTGCATGGAGTTCAAGCCAGAGAGATGGATTTCAGAAAAGGGAAGAATCAAACATGTGCCATCTTACAAATTCTTGGCCTTCAATTCTGGTCCAAGAACTTGCTTAGGAAAACAAGTGGCCTTCATTGAATTGAAAATAGTTGCAGCTGCCATTATTCATAACTACAATATTATTCAACAAACAGGACATGACGTTGTTCCAAATGCTTCCATTATTCTTCATATGAAGCATGGATTCAACGCCAGGGTTACAAAGAGATGGACTTGA

Coding sequence (CDS)

ATGACACTTAGTGTTATTACTCATATTGATGAATCTCATGATCGTATTACTGAAGTCTTACAAGAAGCTGGTTCAACTTTTTTCTTCAAAGGCGTTTGGTTTTCAGGTACGGACTTTCTCCTCACAGTTGACCCTTCAAATATCCACCATATTTTGAGTTCAAATTTCGAACGCTATCCAAAAGGCCCTGATTTCAAGTATCTTTTCGACGTTTTGGGAGATGGAATTTTCAACTCCGATTCAGATGTTTGGAAAAATCAACGCAAAATCGCTCATTTCTTGGTTCATGATGAAAATTTTCTTCAATTCTTGGAGAAAATTACATTGAAGAAAGTGAAGGAAGGGTTGATTCCGGTGCTTGAAAGTGTTTGTGAAAATGGGTTGGTGTTGGATTTACAAGATGTGTTTCAAAGATTCTCTTTTGATTCAACTTGTAGAGACACTCTAAGTTCAGCTCTCTCTTGGTTTTTCTTTTGTCTTTCAAAAAATTCAATCATCGTAGAGAAAATCAGAGAAGAGCTCAAAACAGCAATTCCACCGAATGAAGCTCGAGATCAATGGCGGATATTTTCGATTGAAGAGATAGATAAGTTAGTTTACTTTCATGGAACTTTGTGTGAAGCTCTTAGATTATATCCTCCAGTCCCACTCCAACATAAGGTGTCAGTACAACATGATATCCTCCCAAGTGGTCATCACATAAATCCTAAAACAAAGATTGTGTTTTCTTTATATGCATTGGGAAGAATGAGTGATGTGTGGGGGAAAGATTGCATGGAGTTCAAGCCAGAGAGATGGATTTCAGAAAAGGGAAGAATCAAACATGTGCCATCTTACAAATTCTTGGCCTTCAATTCTGGTCCAAGAACTTGCTTAGGAAAACAAGTGGCCTTCATTGAATTGAAAATAGTTGCAGCTGCCATTATTCATAACTACAATATTATTCAACAAACAGGACATGACGTTGTTCCAAATGCTTCCATTATTCTTCATATGAAGCATGGATTCAACGCCAGGGTTACAAAGAGATGGACTTGA

Protein sequence

MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYPKGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVLESVCENGLVLDLQDVFQRFSFDSTCRDTLSSALSWFFFCLSKNSIIVEKIREELKTAIPPNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTKIVFSLYALGRMSDVWGKDCMEFKPERWISEKGRIKHVPSYKFLAFNSGPRTCLGKQVAFIELKIVAAAIIHNYNIIQQTGHDVVPNASIILHMKHGFNARVTKRWT

Homology

[Close]

BLAST of Lsi01G010690 vs. ExPASy Swiss-Prot
Match: Q9FVS9 (Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.8e-86
Identity = 170/468 (36.32%), Postives = 231/468 (49.36%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           M   ++  I   +D   EVL+    TF+FKG W SGTD L T DP NIHHILSSNF  YP
Sbjct: 41  MLPGMLHQIPRIYDWTVEVLEATNLTFYFKGPWLSGTDMLFTADPRNIHHILSSNFGNYP 100

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGP+FK +FDVLG+GI   D ++W+  RK  H L H+++F++        K+KEGL+P L
Sbjct: 101 KGPEFKKIFDVLGEGILTVDFELWEEMRKSNHALFHNQDFIELSVSSNKSKLKEGLVPFL 160

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           ++  +  ++++LQDVFQRF FD++                                    
Sbjct: 161 DNAAQKNIIIELQDVFQRFMFDTSSILMTGYDPMSLSIEMLEVEFGEAADIGEEAIYYRH 220

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 221 FKPVILWRLQNWIGIGLERKMRTALATVNRMFAKIISSRRKEEISRAKTEPYSKDALTYY 280

Query: 241 ------------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELK 300
                                         RDT SS L+WFF+ LSK+  ++ K+R E+ 
Sbjct: 281 MNVDTSKYKLLKPNKDKFIRDVIFSLVLAGRDTTSSVLTWFFWLLSKHPQVMAKLRHEIN 340

Query: 301 TAIPPNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHIN 344
           T             F  E+++KLVY H  L E++RLYPP+P  HK   + D+LPSGH ++
Sbjct: 341 TK------------FDNEDLEKLVYLHAALSESMRLYPPLPFNHKSPAKPDVLPSGHKVD 400

[Close]

BLAST of Lsi01G010690 vs. ExPASy Swiss-Prot
Match: A0A140IL90 (Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T1 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.5e-72
Identity = 150/460 (32.61%), Postives = 219/460 (47.61%), Query Frame = 0


Query: 3   LSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYPKG 62
           L  I   ++ +D  T  L++ G TF FKG W    D++ T DPSNI+H+ + NFE YPKG
Sbjct: 52  LPAIISNNQFNDFTTARLRKMGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG 111

Query: 63  PDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVLES 122
            +   +FD+ G+ IFN+D D+W + RK+A  ++ D N+          K+   LIP+L+S
Sbjct: 112 -ELGKVFDIFGNNIFNADGDLWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDS 171

Query: 123 VCENGLVLDLQDVFQRFSFDSTC------------------------------------- 182
                  +DLQDVF RF+FD++C                                     
Sbjct: 172 AASKRKPVDLQDVFFRFTFDTSCFSVLAADPESLTMEFPAVPFSKAADQALDAALTRHIT 231

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 232 PRLIWKLKRFFNVGSERTLAVAWKVIDSYIYDKIAELKAKRKLVGKINSYDAVSFYMDNF 291

Query: 243 -------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIPPNEAR-- 302
                              R+T S  ++W F+ L +N  +  KI  ELK+ +  +  R  
Sbjct: 292 NIHDDKFLRDNAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKF 351

Query: 303 -DQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTKIVFS 344
            D + +F    I   +Y H TLCEALR+YPPVP + K + + D+LPSGH +    KI+FS
Sbjct: 352 NDGFALFDSNMIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFS 411

[Close]

BLAST of Lsi01G010690 vs. ExPASy Swiss-Prot
Match: A0A140IL92 (Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T3 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.3e-72
Identity = 150/460 (32.61%), Postives = 218/460 (47.39%), Query Frame = 0


Query: 3   LSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYPKG 62
           L  I    + +D  T  L++ G TF FKG W    D++ T DPSNI+H+ + NFE YPKG
Sbjct: 52  LPAIISNSQFNDFTTARLRKMGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG 111

Query: 63  PDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVLES 122
            +   +FD+ G+ IFN+D D+W + RK+A  ++ D N+          K+   LIP+L+S
Sbjct: 112 -ELGKVFDIFGNNIFNADGDLWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDS 171

Query: 123 VCENGLVLDLQDVFQRFSFDSTC------------------------------------- 182
                  +DLQDVF RF+FD++C                                     
Sbjct: 172 AASKRKPVDLQDVFFRFTFDTSCFSVLAADPESLTMEFPAVPFSKAADQALDAALTRHIT 231

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 232 PRLIWKLKRFFNVGSERTLAVAWKVIDSYIYDKIAELKAKRKLVGKINSYDAVSFYMDNF 291

Query: 243 -------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIPPNEAR-- 302
                              R+T S  ++W F+ L +N  +  KI  ELK+ +  +  R  
Sbjct: 292 NIHDDKFLRDNAFTYLLAQRNTQSLTMAWLFYALFENPKVELKILSELKSIVDESSERRF 351

Query: 303 -DQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTKIVFS 344
            D + +F    I   +Y H TLCEALR+YPPVP + K + + D+LPSGH +    KI+FS
Sbjct: 352 NDGFALFDSNMIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFS 411

[Close]

BLAST of Lsi01G010690 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ9 (Noroxomaritidine synthase 2 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.8e-71
Identity = 149/460 (32.39%), Postives = 218/460 (47.39%), Query Frame = 0


Query: 3   LSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYPKG 62
           L  I   ++ +D  T  L++ G TF FKG W    D++ T DPSNI+H+ + NFE YPKG
Sbjct: 52  LPAIISNNQFNDFTTARLRKMGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG 111

Query: 63  PDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVLES 122
            +   +FD+ G+ IFN+D D+W + RK+A  ++ D N+          K+   LIP+L+S
Sbjct: 112 -ELGKVFDIFGNNIFNADGDLWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDS 171

Query: 123 VCENGLVLDLQDVFQRFSFDSTC------------------------------------- 182
                  +DLQDV  RF+FD++C                                     
Sbjct: 172 AACKRKPVDLQDVLLRFTFDTSCFSVLAADPESLTMEFPPVPFSKAADQALDAALTRHIT 231

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 232 PRLIWKLKRFFNVGSERTLAVAWKVIDSYIYDKIAELKAKRKLVGKINSYDAVSFYMDNF 291

Query: 243 -------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIPPNEAR-- 302
                              R+T S  ++W F+ L +N  +  KI  ELK+ +  +  R  
Sbjct: 292 NIHDDKFLRDNAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKF 351

Query: 303 -DQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTKIVFS 344
            D + +F    I   +Y H TLCEALR+YPPVP + K + + D+LPSGH +    KI+FS
Sbjct: 352 NDGFALFDSNMIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFS 411

[Close]

BLAST of Lsi01G010690 vs. ExPASy Swiss-Prot
Match: A0A2H5AJ00 (Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.7e-71
Identity = 149/460 (32.39%), Postives = 218/460 (47.39%), Query Frame = 0


Query: 3   LSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYPKG 62
           L  I   ++ +D  T  L++ G TF FKG W    D++ T DPSNI+H+ + NFE YPKG
Sbjct: 52  LPAIISNNQFNDFTTARLRKMGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG 111

Query: 63  PDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVLES 122
            +   +FD+ G+ IFN+D D+W + RK+A  ++ D N+          K+   LIP+L+S
Sbjct: 112 -ELGKVFDIFGNNIFNADGDLWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDS 171

Query: 123 VCENGLVLDLQDVFQRFSFDSTC------------------------------------- 182
                  +DLQDV  RF+FD++C                                     
Sbjct: 172 AACKRNPVDLQDVLLRFTFDTSCFSVLAADPESLTMEFPPVPFSKAADQALDAALTRHIT 231

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 232 PRLIWKLKRFFNVGSERTLAVAWKVIDSYIYDKIAELKAKRKLVGKINSYDAVSFYMDNF 291

Query: 243 -------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIPPNEAR-- 302
                              R+T S  ++W F+ L +N  +  KI  ELK+ +  +  R  
Sbjct: 292 NIHDDKFLRDNAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKF 351

Query: 303 -DQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTKIVFS 344
            D + +F    I   +Y H TLCEALR+YPPVP + K + + D+LPSGH +    KI+FS
Sbjct: 352 NDGFALFDSNMIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFS 411

[Close]

BLAST of Lsi01G010690 vs. ExPASy TrEMBL
Match: A0A0A0KFY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487540 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.4e-158
Identity = 292/468 (62.39%), Postives = 313/468 (66.88%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT SV+THI  SHDRITEV+QE GSTFFFKGVWFSG DFLLT DPSNIHHILS+NFERYP
Sbjct: 44  MTPSVVTHIHRSHDRITEVIQEVGSTFFFKGVWFSGMDFLLTADPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDS  WK+QRK A  LVHDE FLQFLEKITL KVK G++PVL
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSYAWKDQRKTARSLVHDEKFLQFLEKITLNKVKTGIVPVL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           + VCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 DGVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLQSLSLESPAVPFSKAMDDVEEVIFLRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKKIWEFQKKLQIGQPMRLKQAWKIIDETIAKLIALKRGSLKNQVNKEGDEQGRGVDL 283

Query: 241 ---------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAI 300
                                      RDTLSSALSWFFFCLSKN  +V+ IREELKT I
Sbjct: 284 IASYMINNTNKDDKFFRDTVLNFMIAGRDTLSSALSWFFFCLSKNPTVVKMIREELKTTI 343

Query: 301 PPNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKT 346
           P NEA DQ RIFS+EE++KLVYFH TLCEALRLYPPVP QHKV+ QHDILPSGHHI PKT
Sbjct: 344 PSNEACDQLRIFSMEEVNKLVYFHATLCEALRLYPPVPFQHKVATQHDILPSGHHIKPKT 403

[Close]

BLAST of Lsi01G010690 vs. ExPASy TrEMBL
Match: A0A0A0KJ78 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 287/467 (61.46%), Postives = 313/467 (67.02%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT +VI HI   HDR+TEVLQ+   TFFFKG+WFSG DFL TVDPSNIHHILS+NFERYP
Sbjct: 44  MTPTVIAHIHRPHDRVTEVLQQTAFTFFFKGIWFSGMDFLFTVDPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDSDVWKNQRK AH+LV   NFLQFLEKITL KVK GL+P+L
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSDVWKNQRKTAHYLVRHHNFLQFLEKITLNKVKTGLVPIL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           +SVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 DSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLNSLSLEFPEVPFSKAMDDAEEVIFVRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKTIWEFQKKLQIGQPKRLKQAWEIIDETIAKLIASKRKSLKNEMKEEDDNGKEGVDL 283

Query: 241 --------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIP 300
                                     RDTLSSALSWFFFCLS +  +VEKIREEL+T IP
Sbjct: 284 ITSYITNIENDDEFLRDTVLNFMIAGRDTLSSALSWFFFCLSNHPTVVEKIREELRTTIP 343

Query: 301 PNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTK 346
            NEA DQ RIFSIEE+D LVYFHGTLCEALRLYPPVPLQHKVS+QHDILPSGHHI PKTK
Sbjct: 344 TNEAYDQRRIFSIEEVDNLVYFHGTLCEALRLYPPVPLQHKVSIQHDILPSGHHIKPKTK 403

[Close]

BLAST of Lsi01G010690 vs. ExPASy TrEMBL
Match: A0A5D3CM15 (Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005590 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.3e-156
Identity = 286/467 (61.24%), Postives = 313/467 (67.02%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT +VI H    HDR+TEVLQ+   TFFFKG+WFSGTDFL TVDPSNIHHILS+NFERYP
Sbjct: 44  MTPTVIAHFHRPHDRVTEVLQQTAFTFFFKGIWFSGTDFLFTVDPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDSDVWK+QRK AH LV  E+FLQFLEKITL KVK GL+PVL
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSDVWKSQRKTAHHLVRHESFLQFLEKITLNKVKTGLVPVL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           +SVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 DSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLHSLSLESPEVPFSKAMDDAEEVIFLRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKTIWEFQKKLQIGQPKRLKQAWEIIDETIAKLIASKRKSLKNQMKEEGGDDKEGVDL 283

Query: 241 --------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIP 300
                                     RDTLSSALSWFFFC+S N  +VEKIREEL+T+IP
Sbjct: 284 ITSYITNIEYDDEFLRDTVLNFMIAGRDTLSSALSWFFFCVSNNPTVVEKIREELRTSIP 343

Query: 301 PNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTK 346
            NEA DQ RIFS+EE+D LVY HGTLCEALRLYPPVPLQHKVS QHDILPSGHHI PKTK
Sbjct: 344 TNEAYDQRRIFSLEEVDNLVYLHGTLCEALRLYPPVPLQHKVSTQHDILPSGHHIKPKTK 403

[Close]

BLAST of Lsi01G010690 vs. ExPASy TrEMBL
Match: A0A1S3B0F5 (alkane hydroxylase MAH1-like OS=Cucumis melo OX=3656 GN=LOC103484695 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.3e-156
Identity = 286/467 (61.24%), Postives = 313/467 (67.02%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT +VI H    HDR+TEVLQ+   TFFFKG+WFSGTDFL TVDPSNIHHILS+NFERYP
Sbjct: 44  MTPTVIAHFHRPHDRVTEVLQQTAFTFFFKGIWFSGTDFLFTVDPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDSDVWK+QRK AH LV  E+FLQFLEKITL KVK GL+PVL
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSDVWKSQRKTAHHLVRHESFLQFLEKITLNKVKTGLVPVL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           +SVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 DSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLHSLSLESPEVPFSKAMDDAEEVIFLRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKTIWEFQKKLQIGQPKRLKQAWEIIDETIAKLIASKRKSLKNQMKEEGGDDKEGVDL 283

Query: 241 --------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIP 300
                                     RDTLSSALSWFFFC+S N  +VEKIREEL+T+IP
Sbjct: 284 ITSYITNIEYDDEFLRDTVLNFMIAGRDTLSSALSWFFFCVSNNPTVVEKIREELRTSIP 343

Query: 301 PNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTK 346
            NEA DQ RIFS+EE+D LVY HGTLCEALRLYPPVPLQHKVS QHDILPSGHHI PKTK
Sbjct: 344 TNEAYDQRRIFSLEEVDNLVYLHGTLCEALRLYPPVPLQHKVSTQHDILPSGHHIKPKTK 403

[Close]

BLAST of Lsi01G010690 vs. ExPASy TrEMBL
Match: A0A5D3CNM3 (Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005580 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 291/470 (61.91%), Postives = 315/470 (67.02%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT SV+TH   SHDRITEV+QEAGSTFFFKGVWFSG DFLLTVDPSNIHHILS+NFERYP
Sbjct: 44  MTPSVVTHFYRSHDRITEVIQEAGSTFFFKGVWFSGMDFLLTVDPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDS  WK+QRK A  LVHDENFLQFLEKITLKKVK  L+PVL
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSYAWKDQRKTARSLVHDENFLQFLEKITLKKVKTALVPVL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           +SVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 QSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLQSLSLEFPEVPFSKAMDDAEEVIFLRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKKIWEFQKKLQIGQPMRLKQAWEIIDETIAKLIALKRGSLKNQVNKEGDEQGAGGVD 283

Query: 241 ----------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTA 300
                                       RDTLSSALSWFFFCL KN  +V+KIREEL+T 
Sbjct: 284 LIKSYMINNTNKDDKFLRDTVLNFMIAGRDTLSSALSWFFFCLLKNPTVVKKIREELRTT 343

Query: 301 IPPNEARD-QWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINP 346
           IP N A D Q R+FS+EE++KLVYFH TLCEALRLYPPVPLQHKV++QHDILPSGHHI P
Sbjct: 344 IPTNGACDLQQRVFSMEEVNKLVYFHATLCEALRLYPPVPLQHKVAMQHDILPSGHHIKP 403

[Close]

BLAST of Lsi01G010690 vs. NCBI nr
Match: XP_038882620.1 (alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 594.7 bits (1532), Expect = 5.0e-166
Identity = 303/466 (65.02%), Postives = 320/466 (68.67%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT +V+ HI+ +HDR+TEVLQ AGSTFFFKGVWFSG DFL TVDPSNIHHILSSNFERYP
Sbjct: 43  MTPTVVAHINRAHDRVTEVLQAAGSTFFFKGVWFSGMDFLFTVDPSNIHHILSSNFERYP 102

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDSDVWKNQRK AH LVHDENFLQFLEKITLKKVKEGL+P+L
Sbjct: 103 KGPDFKYIFEVLGDGIFNSDSDVWKNQRKTAHSLVHDENFLQFLEKITLKKVKEGLVPIL 162

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           ESVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 163 ESVCENGSVLDLQDLFQRFSFDSTCMLVTGFDLNSLSLQLPEVPFSKAMDDVEEVIFLRH 222

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 223 VFPKKIWEFHKKLQIGQPKRLKQAWEIIDETIAKLIASKRKSMKNQMKEEADEQGIGVDL 282

Query: 241 --------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIP 300
                                     RDTLSSALSWFFFCLS N I+V  IREELKT IP
Sbjct: 283 ITSYITNTKNDDEFLRDTVLNFMIAGRDTLSSALSWFFFCLSNNPIVVANIREELKTTIP 342

Query: 301 PNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTK 346
            NE RDQ RIFSIEE+DKLVYFHG+LCEALRLYPPVPLQHKVSVQHDILP+GHHINPKTK
Sbjct: 343 TNEVRDQLRIFSIEEVDKLVYFHGSLCEALRLYPPVPLQHKVSVQHDILPTGHHINPKTK 402

[Close]

BLAST of Lsi01G010690 vs. NCBI nr
Match: XP_011658477.1 (alkane hydroxylase MAH1 [Cucumis sativus])

HSP 1 Score: 568.2 bits (1463), Expect = 5.0e-158
Identity = 292/468 (62.39%), Postives = 313/468 (66.88%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT SV+THI  SHDRITEV+QE GSTFFFKGVWFSG DFLLT DPSNIHHILS+NFERYP
Sbjct: 44  MTPSVVTHIHRSHDRITEVIQEVGSTFFFKGVWFSGMDFLLTADPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDS  WK+QRK A  LVHDE FLQFLEKITL KVK G++PVL
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSYAWKDQRKTARSLVHDEKFLQFLEKITLNKVKTGIVPVL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           + VCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 DGVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLQSLSLESPAVPFSKAMDDVEEVIFLRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKKIWEFQKKLQIGQPMRLKQAWKIIDETIAKLIALKRGSLKNQVNKEGDEQGRGVDL 283

Query: 241 ---------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAI 300
                                      RDTLSSALSWFFFCLSKN  +V+ IREELKT I
Sbjct: 284 IASYMINNTNKDDKFFRDTVLNFMIAGRDTLSSALSWFFFCLSKNPTVVKMIREELKTTI 343

Query: 301 PPNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKT 346
           P NEA DQ RIFS+EE++KLVYFH TLCEALRLYPPVP QHKV+ QHDILPSGHHI PKT
Sbjct: 344 PSNEACDQLRIFSMEEVNKLVYFHATLCEALRLYPPVPFQHKVATQHDILPSGHHIKPKT 403

[Close]

BLAST of Lsi01G010690 vs. NCBI nr
Match: XP_011657793.1 (alkane hydroxylase MAH1 [Cucumis sativus] >KGN48432.1 hypothetical protein Csa_004344 [Cucumis sativus])

HSP 1 Score: 565.1 bits (1455), Expect = 4.2e-157
Identity = 287/467 (61.46%), Postives = 313/467 (67.02%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT +VI HI   HDR+TEVLQ+   TFFFKG+WFSG DFL TVDPSNIHHILS+NFERYP
Sbjct: 44  MTPTVIAHIHRPHDRVTEVLQQTAFTFFFKGIWFSGMDFLFTVDPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDSDVWKNQRK AH+LV   NFLQFLEKITL KVK GL+P+L
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSDVWKNQRKTAHYLVRHHNFLQFLEKITLNKVKTGLVPIL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           +SVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 DSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLNSLSLEFPEVPFSKAMDDAEEVIFVRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKTIWEFQKKLQIGQPKRLKQAWEIIDETIAKLIASKRKSLKNEMKEEDDNGKEGVDL 283

Query: 241 --------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIP 300
                                     RDTLSSALSWFFFCLS +  +VEKIREEL+T IP
Sbjct: 284 ITSYITNIENDDEFLRDTVLNFMIAGRDTLSSALSWFFFCLSNHPTVVEKIREELRTTIP 343

Query: 301 PNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTK 346
            NEA DQ RIFSIEE+D LVYFHGTLCEALRLYPPVPLQHKVS+QHDILPSGHHI PKTK
Sbjct: 344 TNEAYDQRRIFSIEEVDNLVYFHGTLCEALRLYPPVPLQHKVSIQHDILPSGHHIKPKTK 403

[Close]

BLAST of Lsi01G010690 vs. NCBI nr
Match: XP_008440138.1 (PREDICTED: alkane hydroxylase MAH1-like [Cucumis melo] >TYK12963.1 alkane hydroxylase MAH1-like [Cucumis melo var. makuwa])

HSP 1 Score: 561.6 bits (1446), Expect = 4.7e-156
Identity = 286/467 (61.24%), Postives = 313/467 (67.02%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT +VI H    HDR+TEVLQ+   TFFFKG+WFSGTDFL TVDPSNIHHILS+NFERYP
Sbjct: 44  MTPTVIAHFHRPHDRVTEVLQQTAFTFFFKGIWFSGTDFLFTVDPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDSDVWK+QRK AH LV  E+FLQFLEKITL KVK GL+PVL
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSDVWKSQRKTAHHLVRHESFLQFLEKITLNKVKTGLVPVL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           +SVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 DSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLHSLSLESPEVPFSKAMDDAEEVIFLRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKTIWEFQKKLQIGQPKRLKQAWEIIDETIAKLIASKRKSLKNQMKEEGGDDKEGVDL 283

Query: 241 --------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTAIP 300
                                     RDTLSSALSWFFFC+S N  +VEKIREEL+T+IP
Sbjct: 284 ITSYITNIEYDDEFLRDTVLNFMIAGRDTLSSALSWFFFCVSNNPTVVEKIREELRTSIP 343

Query: 301 PNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINPKTK 346
            NEA DQ RIFS+EE+D LVY HGTLCEALRLYPPVPLQHKVS QHDILPSGHHI PKTK
Sbjct: 344 TNEAYDQRRIFSLEEVDNLVYLHGTLCEALRLYPPVPLQHKVSTQHDILPSGHHIKPKTK 403

[Close]

BLAST of Lsi01G010690 vs. NCBI nr
Match: TYK12962.1 (alkane hydroxylase MAH1-like [Cucumis melo var. makuwa])

HSP 1 Score: 560.8 bits (1444), Expect = 8.0e-156
Identity = 291/470 (61.91%), Postives = 315/470 (67.02%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           MT SV+TH   SHDRITEV+QEAGSTFFFKGVWFSG DFLLTVDPSNIHHILS+NFERYP
Sbjct: 44  MTPSVVTHFYRSHDRITEVIQEAGSTFFFKGVWFSGMDFLLTVDPSNIHHILSANFERYP 103

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGPDFKY+F+VLGDGIFNSDS  WK+QRK A  LVHDENFLQFLEKITLKKVK  L+PVL
Sbjct: 104 KGPDFKYIFEVLGDGIFNSDSYAWKDQRKTARSLVHDENFLQFLEKITLKKVKTALVPVL 163

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           +SVCENG VLDLQD+FQRFSFDSTC                                   
Sbjct: 164 QSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLQSLSLEFPEVPFSKAMDDAEEVIFLRH 223

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 224 FFPKKIWEFQKKLQIGQPMRLKQAWEIIDETIAKLIALKRGSLKNQVNKEGDEQGAGGVD 283

Query: 241 ----------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKTA 300
                                       RDTLSSALSWFFFCL KN  +V+KIREEL+T 
Sbjct: 284 LIKSYMINNTNKDDKFLRDTVLNFMIAGRDTLSSALSWFFFCLLKNPTVVKKIREELRTT 343

Query: 301 IPPNEARD-QWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHINP 346
           IP N A D Q R+FS+EE++KLVYFH TLCEALRLYPPVPLQHKV++QHDILPSGHHI P
Sbjct: 344 IPTNGACDLQQRVFSMEEVNKLVYFHATLCEALRLYPPVPLQHKVAMQHDILPSGHHIKP 403

[Close]

BLAST of Lsi01G010690 vs. TAIR 10
Match: AT2G23180.1 (cytochrome P450, family 96, subfamily A, polypeptide 1 )

HSP 1 Score: 351.7 bits (901), Expect = 6.8e-97
Identity = 191/473 (40.38%), Postives = 250/473 (52.85%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           M   ++  I   +D +TE+L+ +  T+ FKG  F G D L+TVDP+NIHHI+SSNF  YP
Sbjct: 42  MLPGLLVEIPRVYDFVTELLEASNLTYPFKGPCFGGLDMLITVDPANIHHIMSSNFANYP 101

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KG +FK +FDVLGDGIFN+DS++WK+ RK A  ++  ++F +F  +  + K+++GL+P+L
Sbjct: 102 KGTEFKKIFDVLGDGIFNADSELWKDLRKSAQSMMTHQDFQRFTLRTIMSKLEKGLVPLL 161

Query: 121 ESVCENGLVLDLQDVFQRFSFD-------------------------------------- 180
           + V E   V+DLQDVFQRF+FD                                      
Sbjct: 162 DYVAEKKQVVDLQDVFQRFTFDTSFVLATGVDPGCLSTEMPQIEFARALDEAEEAIFFRH 221

Query: 181 --------------------------------STC------------------------- 240
                                           S C                         
Sbjct: 222 VKPEIVWKMQRFIGFGDELKMKKAHSTFDRVCSKCIASKRDEITNGVINIDSSSKDLLMC 281

Query: 241 -----------------------------------RDTLSSALSWFFFCLSKNSIIVEKI 300
                                              RDT SSAL+WFF+ LSKN   + KI
Sbjct: 282 YMNVDTICHTTKYKLLNPSDDKFLRDMILSFMLAGRDTTSSALTWFFWLLSKNPKAITKI 341

Query: 301 REELKTAIPPNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPS 344
           R+E+ T + P    + +  F+ +E++KLVY HG LCEALRLYPPVP QHK   + D+LPS
Sbjct: 342 RQEINTQLSPR--TNDFDSFNAQELNKLVYVHGALCEALRLYPPVPFQHKSPTKSDVLPS 401

[Close]

BLAST of Lsi01G010690 vs. TAIR 10
Match: AT4G39480.1 (cytochrome P450, family 96, subfamily A, polypeptide 9 )

HSP 1 Score: 340.5 bits (872), Expect = 1.6e-93
Identity = 185/473 (39.11%), Postives = 244/473 (51.59%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           M   ++T +   +D ITEVL++    + F G +  G D L TVDP+NIHHI+SSNF  YP
Sbjct: 41  MLPGLLTVLPRVYDFITEVLEDGNLNYLFIGPFLGGIDMLFTVDPANIHHIMSSNFANYP 100

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KG +FK LFDVLGDGIFN+DSD+WK+ RK +  +++  +F +F    +L K+++GL+P+L
Sbjct: 101 KGTEFKKLFDVLGDGIFNADSDLWKDLRKSSQSMMNHPDFQRFSLATSLSKLEKGLVPLL 160

Query: 121 ESVCENGLVLDLQDVFQRFSFDST------------------------------------ 180
           + V +  LV+DLQDVFQRF+FD+T                                    
Sbjct: 161 DHVAKEKLVVDLQDVFQRFTFDTTFVLATGYDPGCLSVEMPEIEFARALDDAEEAIFYRH 220

Query: 181 ----------------------------------C------------------------- 240
                                             C                         
Sbjct: 221 FKPEVVWKMQRLIGVGAELKLKRAHAIFDRVCSKCIASKRDEISQGIDSSSSKDLLMSSI 280

Query: 241 -----------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELKT 300
                                        RDT  SAL+WFF+ L  N   + KIR+E+ T
Sbjct: 281 NVDTTKYKLLNPSDDRFLRDTILSFMLAGRDTTGSALTWFFWLLCNNQEAMTKIRQEINT 340

Query: 301 AIPPNEARDQWRI------FSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPS 344
            + P    D   +      F+ +E+ KLVY HG +CEALRLYPPVP  HK   + D+LPS
Sbjct: 341 NLFPRNKTDDGSVSYDSDSFNPQEVKKLVYLHGAVCEALRLYPPVPFNHKSPAKPDVLPS 400

[Close]

BLAST of Lsi01G010690 vs. TAIR 10
Match: AT4G39490.1 (cytochrome P450, family 96, subfamily A, polypeptide 10 )

HSP 1 Score: 331.3 bits (848), Expect = 9.6e-91
Identity = 182/476 (38.24%), Postives = 243/476 (51.05%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           M   ++  I   +D ITE+L+    T+   G  F+  D L+TVDP+NIHHI+SSNF  YP
Sbjct: 41  MIPGLLVEIHRVYDFITEILEVTNLTYPCTGPCFANLDMLVTVDPANIHHIMSSNFANYP 100

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGP+FK LFD+LGDGIFN+DS++WK+ RK A  ++ +  F +F    +LKK+++GL+P+L
Sbjct: 101 KGPEFKKLFDILGDGIFNADSELWKDLRKSAQSMMMNPEFQKFSLATSLKKLEKGLVPLL 160

Query: 121 ESVCENGLVLDLQDVFQRFSFDST------------------------------------ 180
           + V +  L +DLQD+FQRF+FD+T                                    
Sbjct: 161 DHVAKEKLAVDLQDMFQRFTFDTTFVLATGYDPGCLSVEMPEVEFARALDDAEEAIFFRH 220

Query: 181 -------------------------------C---------------------------- 240
                                          C                            
Sbjct: 221 VKPEIFWRLQGLLGLGDEKKMTKARSTLDRVCSKYIAIKRDEVSRGTNNVDSHSKDLLTS 280

Query: 241 -------------------------------RDTLSSALSWFFFCLSKNSIIVEKIREEL 300
                                          RDT  S L+WFF+ L KN  ++ KIR+E+
Sbjct: 281 YMNLDTTKYKLLNPSDERFLRDTILTFMLAGRDTTGSGLTWFFWLLIKNPEVIAKIRQEI 340

Query: 301 KTAIPPNEARD-------QWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDI 344
            TA+      D           FS +E+ KLVY HG +CE+LRLYPPVP QHK   + D+
Sbjct: 341 NTALFQRSKVDDDASNNNDSDSFSPQELKKLVYLHGAICESLRLYPPVPFQHKSPTKPDV 400

[Close]

BLAST of Lsi01G010690 vs. TAIR 10
Match: AT1G57750.1 (cytochrome P450, family 96, subfamily A, polypeptide 15 )

HSP 1 Score: 320.9 bits (821), Expect = 1.3e-87
Identity = 170/468 (36.32%), Postives = 231/468 (49.36%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           M   ++  I   +D   EVL+    TF+FKG W SGTD L T DP NIHHILSSNF  YP
Sbjct: 41  MLPGMLHQIPRIYDWTVEVLEATNLTFYFKGPWLSGTDMLFTADPRNIHHILSSNFGNYP 100

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KGP+FK +FDVLG+GI   D ++W+  RK  H L H+++F++        K+KEGL+P L
Sbjct: 101 KGPEFKKIFDVLGEGILTVDFELWEEMRKSNHALFHNQDFIELSVSSNKSKLKEGLVPFL 160

Query: 121 ESVCENGLVLDLQDVFQRFSFDSTC----------------------------------- 180
           ++  +  ++++LQDVFQRF FD++                                    
Sbjct: 161 DNAAQKNIIIELQDVFQRFMFDTSSILMTGYDPMSLSIEMLEVEFGEAADIGEEAIYYRH 220

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 221 FKPVILWRLQNWIGIGLERKMRTALATVNRMFAKIISSRRKEEISRAKTEPYSKDALTYY 280

Query: 241 ------------------------------RDTLSSALSWFFFCLSKNSIIVEKIREELK 300
                                         RDT SS L+WFF+ LSK+  ++ K+R E+ 
Sbjct: 281 MNVDTSKYKLLKPNKDKFIRDVIFSLVLAGRDTTSSVLTWFFWLLSKHPQVMAKLRHEIN 340

Query: 301 TAIPPNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGHHIN 344
           T             F  E+++KLVY H  L E++RLYPP+P  HK   + D+LPSGH ++
Sbjct: 341 TK------------FDNEDLEKLVYLHAALSESMRLYPPLPFNHKSPAKPDVLPSGHKVD 400

[Close]

BLAST of Lsi01G010690 vs. TAIR 10
Match: AT1G65340.1 (cytochrome P450, family 96, subfamily A, polypeptide 3 )

HSP 1 Score: 319.7 bits (818), Expect = 2.9e-87
Identity = 169/470 (35.96%), Postives = 235/470 (50.00%), Query Frame = 0


Query: 1   MTLSVITHIDESHDRITEVLQEAGSTFFFKGVWFSGTDFLLTVDPSNIHHILSSNFERYP 60
           M   ++  +   +D ITEVL+    TF FKG   SG D LLTVDP NIH+ILSSNF  YP
Sbjct: 42  MLPGLLLQVPRIYDWITEVLEATDMTFCFKGPCLSGMDILLTVDPVNIHYILSSNFANYP 101

Query: 61  KGPDFKYLFDVLGDGIFNSDSDVWKNQRKIAHFLVHDENFLQFLEKITLKKVKEGLIPVL 120
           KG +FK +F+V+GD IFN DS +W++ R  +H +  +++F  F    +++K+++GL+P+L
Sbjct: 102 KGMEFKKIFEVVGDSIFNVDSGLWEDMRNSSHAIFSNQDFQMFWVSTSVRKLRQGLVPIL 161

Query: 121 ESVCENGLVLDLQDVFQRFSFDST------------------------------------ 180
           E+  +  +++DLQD+FQRF FD++                                    
Sbjct: 162 ENAADKNILVDLQDLFQRFLFDTSLILMTGYDPKCLSVEMPKVEFGDAVDGVSDGVFYRH 221

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 222 VKPVFLWRLQYLIGVGVEKRLKRGLAVFDQLLEKIITAKREEINSHGTHHPSRGEAIDVL 281

Query: 241 --------------------------------CRDTLSSALSWFFFCLSKNSIIVEKIRE 300
                                            RDT SSAL+WFF+ +SKN   + KIR+
Sbjct: 282 TYYMTMDTTKYKYLEPSDDRFIKDTILGFLIAARDTTSSALTWFFWLMSKNPEAINKIRQ 341

Query: 301 ELKTAIPPNEARDQWRIFSIEEIDKLVYFHGTLCEALRLYPPVPLQHKVSVQHDILPSGH 343
           E+   +P          F   +++KLVY HG +CE LRLYPPVP  HK   + D+LPSGH
Sbjct: 342 EVNKKMPR---------FDPADLEKLVYLHGAVCETLRLYPPVPFNHKSPAKPDVLPSGH 401

The following BLAST results are available for this feature:

BLAST of Lsi01G010690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FVS9	1.8e-86	36.32	Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1	[more]
A0A140IL90	2.5e-72	32.61	Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 G...	[more]
A0A140IL92	3.3e-72	32.61	Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222...	[more]
A0A2H5AIZ9	2.8e-71	32.39	Noroxomaritidine synthase 2 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T2 PE=...	[more]
A0A2H5AJ00	3.7e-71	32.39	Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=...	[more]

BLAST of Lsi01G010690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFY4	2.4e-158	62.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487540 PE=3 SV=1	[more]
A0A0A0KJ78	2.0e-157	61.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1	[more]
A0A5D3CM15	2.3e-156	61.24	Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3B0F5	2.3e-156	61.24	alkane hydroxylase MAH1-like OS=Cucumis melo OX=3656 GN=LOC103484695 PE=3 SV=1	[more]
A0A5D3CNM3	3.9e-156	61.91	Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Lsi01G010690 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882620.1	5.0e-166	65.02	alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida]	[more]
XP_011658477.1	5.0e-158	62.39	alkane hydroxylase MAH1 [Cucumis sativus]	[more]
XP_011657793.1	4.2e-157	61.46	alkane hydroxylase MAH1 [Cucumis sativus] >KGN48432.1 hypothetical protein Csa_0...	[more]
XP_008440138.1	4.7e-156	61.24	PREDICTED: alkane hydroxylase MAH1-like [Cucumis melo] >TYK12963.1 alkane hydrox...	[more]
TYK12962.1	8.0e-156	61.91	alkane hydroxylase MAH1-like [Cucumis melo var. makuwa]	[more]

BLAST of Lsi01G010690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23180.1	6.8e-97	40.38	cytochrome P450, family 96, subfamily A, polypeptide 1	[more]
AT4G39480.1	1.6e-93	39.11	cytochrome P450, family 96, subfamily A, polypeptide 9	[more]
AT4G39490.1	9.6e-91	38.24	cytochrome P450, family 96, subfamily A, polypeptide 10	[more]
AT1G57750.1	1.3e-87	36.32	cytochrome P450, family 96, subfamily A, polypeptide 15	[more]
AT1G65340.1	2.9e-87	35.96	cytochrome P450, family 96, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 281..291 score: 45.23 coord: 291..314 score: 26.43 coord: 202..220 score: 24.45 coord: 153..179 score: 26.92
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 144..161 score: 28.98 coord: 282..291 score: 49.14 coord: 291..302 score: 41.67 coord: 203..214 score: 36.72
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 12..146 e-value: 1.1E-6 score: 27.7 coord: 147..324 e-value: 1.2E-38 score: 133.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 146..345 e-value: 2.6E-52 score: 179.8
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 2..145 e-value: 7.5E-18 score: 66.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 12..343
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 146..344 coord: 4..145
None	No IPR available	PANTHER	PTHR24296:SF209	ALKANE HYDROXYLASE MAH1-LIKE	coord: 146..344 coord: 4..145
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 284..293

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G010690.1	Lsi01G010690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen