Lsi01G010120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G010120
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPyridoxal phosphate-dependent transferases superfamily protein
Locationchr01: 8398631 .. 8401447 (+)
RNA-Seq ExpressionLsi01G010120
SyntenyLsi01G010120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCTTTCACTATGGAAAAATCTGTCTCATTGCGCTGCTGCTCTATTAATGGACAAGAAGGGCCGAAGAAGAGATGGGTCTGCCACCGGACATGACTCCAAGAAAAACTCATCAATTCTTCGAAAATTGCAAGAGAACAAGCTCAGGGAAGCCCTTGAAGAAGCATCCGAAAATGGGTCTCTCTTCAAATCGCAAGATATTGATGAATCCGAATCGCTTGGCGACCAGGATGACGGCGGCGGCTTGGGCCGGTCGCGTTCGCTCGCTAGACTCCACGCGCAGCGGGAATTCCTACGCGCCACTGCCCTCGCCGCTGAGCGCACTTTCGAGTCCGAAGACGCCATTCCTGACCTCCATGAATCTTTCTCCAAGTTCCTTACTATGTACCCGAATTACCAGTCCTCCGAGAAGATCGATCAACTGAGGTCCAACGAGTATTCGCATCTCTCTTCAAAGGTATGCCTCGATTACTGTGGCTTCGGACTTTTTTCTTATGTTCAAACACTTCACTATTGGGAGTCCTCCACATTTAGCTTGTCTGAGATTACTGCAAATCTGAGCAACCATGCTCTTTATGGAGGTGCTGAAAAGGGGACTGTTGAACATGATATAAAGCTTAAGATCATGGACTATTTGAACATTCCTGAGCATGAGTATGGTCTTGTGTTTACTGTGAGTAGAGGGTCGGCTTTTAAATTGCTTGCTGATTCATATCCTTTTCATACCAACAAAAAATTGTTAACCATGTTTGATTATGAGAGCCAGTCTGTGAGTTGGATGGGTCAGTGTGCTAGAGAGAAAGGTGCAAAAGTGTATAGCGCCTGGTTCAAATGGCCATCCCTGAAACTGTGCTCCACTGATTTGAGAAAACAGATTTCGAATAAGAGGAGAAAGAAGAAGGACTGTGCTACTGGCCTTTTTGTGTTTCCAGTTCAATCTAGAGTGACAGGGGCTAAGTATTCTTATCAATGGATGGCTCTAGCACAACAGAACAACTGGCATGTGCTGCTTGATGCTGGATCTTTAGGTCCTAAAGACATGGATTCGCTTGGACTGTCCCTTTTCCGGCCAGATTTCATTGTTACATCGTTCTATAGAGTTTTTGGATTTGATCCAACCGGTTTTGGATGTCTACTAATCAAGAAATCAGTAATGGCAAGTTTACAGAATCATTCTGGGAGCACAGGATCAGGAATGGTGAAGATAACTCCTGAATATCCTTTATACCTGAGTGATTCAGCTGACGGTCTTGATGGATTCGCTGGGATTGAAGATGATGGAGTCTCTGAGAATGTTGATAAAGCCTCTGAAACACGTCAAAAATCTCAGCTGCCTGCTTTCTCGGGTGCGTTTACATCTTCTCAGGTTAGAGAGGTATTTGAAACTGAGATGGATAGTTCTGAGAGAGATGGTACGAGCACTATATTTGAGGAATCGGAGAGTATCTCTGTTGGAGAGGTGATGAAGAGCCCAGTATTCAGTGAAGATGAGTCATCTGATAATTCATTGTGGATTGATTTAGGCCACAGTCCACTTGGGTCTGATAATGCTGGCTTCAGCAAACATGAAATTGCCTCCCCATTGCCCCCATATTGGTTTGCATATCGGAAAAATAGGCGACAATCACCCAAACCAACTTCGAAAATATACAGCAGCCCACTTTATGATGACAAAGAAGTAAATTCTAGGCCAGGTGATGAAAGAAATATGCTATCATTTGATGCAGCTGTCATGTCAGTTTCTCAAGAGCTTGATCGTTACAAAGAGGTTGGTGGAGGTAAAGATTCAGGTATGAGCCCTGCTTCACAGAGTGGTAAAAAGTGTATAGCGCATACGGACGTGTTGGAGGTAGACAAAACAAGCAAGACACTTTCAAATGGTATATCAAGTTGTGATGTTAAGAGATCTCATCTTGACAATTCAACTTCTCTATCCCAGCACCATAACTTAGAGAACGGTTCGACCTCTGAGATCTGCTCAGAGATTAAGGAGAGTGCAATAAGACGTGAAACTGAAGGTGAATTCAGGTTGCTGGGATGCCGAGGGAATAGATTTGCCGGTGGGAGGTTTTTTGGAGTAGATGAATCTGAAGTGCAGAACAAGGGAAGAAGAGTCTCTTTTGGCATTGAAGACAATGGTAAAGAGCACCAGAGCTGTAATTTGGAGCCAGGTGAAACGTCTGTTACTAGCTTTGATGATGATGAAGGTACCAGTGACGGTGAATATGGTGATGGAGAAGATTGGGACAGGAGAGAACCTGAGATTATATGCCGCCATATTGATCATATCGACTTGTTGGGTCTCAATAAGACTACTCTCAGATTAAGATTTCTGATCAATTGGCTCGTGACGTCACTACTACAACTTAGGTTACCCGATTCTGATGGAAGCAGCAGAGCAAATCTTGTTCAAATTTATGGCCCTAAAATAAAATACGAAAGGGGTGCTGCCGTAGCATTCAATGTGAGAGACAAAGTTAGGGGGCTAATCAACCCAGAAATTGTACAGAAGCTGGCTGAAAGAGAGGGTATATCTCTAGGAATAGGTTTTCTTAGTCACATACGTATCGTCGACAATCCAAGACATCATAAAGGTTTGAACCTAGATGACACCACCCTTTGCAGACCAATGGAAAATGGCAAGCTTGGTGGAAAGAGCGGGTTAATGCGGGTTGAAGTCGTGACAGCTTCTCTGGGATTTTTGACCAATTTCGAAGATGTTTACAGATTATGGGCTTTTGTAGCAAAGTTTCTTAATCCAACCTTTATCAAAGAGGGTGCTCTTCCTACAGTTGAAGAAGGTTCAGAAATATAA

mRNA sequence

ATGCATCTTTCACTATGGAAAAATCTGTCTCATTGCGCTGCTGCTCTATTAATGGACAAGAAGGGCCGAAGAAGAGATGGGTCTGCCACCGGACATGACTCCAAGAAAAACTCATCAATTCTTCGAAAATTGCAAGAGAACAAGCTCAGGGAAGCCCTTGAAGAAGCATCCGAAAATGGGTCTCTCTTCAAATCGCAAGATATTGATGAATCCGAATCGCTTGGCGACCAGGATGACGGCGGCGGCTTGGGCCGGTCGCGTTCGCTCGCTAGACTCCACGCGCAGCGGGAATTCCTACGCGCCACTGCCCTCGCCGCTGAGCGCACTTTCGAGTCCGAAGACGCCATTCCTGACCTCCATGAATCTTTCTCCAAGTTCCTTACTATGTACCCGAATTACCAGTCCTCCGAGAAGATCGATCAACTGAGGTCCAACGAGTATTCGCATCTCTCTTCAAAGGTATGCCTCGATTACTGTGGCTTCGGACTTTTTTCTTATGTTCAAACACTTCACTATTGGGAGTCCTCCACATTTAGCTTGTCTGAGATTACTGCAAATCTGAGCAACCATGCTCTTTATGGAGGTGCTGAAAAGGGGACTGTTGAACATGATATAAAGCTTAAGATCATGGACTATTTGAACATTCCTGAGCATGAGTATGGTCTTGTGTTTACTGTGAGTAGAGGGTCGGCTTTTAAATTGCTTGCTGATTCATATCCTTTTCATACCAACAAAAAATTGTTAACCATGTTTGATTATGAGAGCCAGTCTGTGAGTTGGATGGGTCAGTGTGCTAGAGAGAAAGGTGCAAAAGTGTATAGCGCCTGGTTCAAATGGCCATCCCTGAAACTGTGCTCCACTGATTTGAGAAAACAGATTTCGAATAAGAGGAGAAAGAAGAAGGACTGTGCTACTGGCCTTTTTGTGTTTCCAGTTCAATCTAGAGTGACAGGGGCTAAGTATTCTTATCAATGGATGGCTCTAGCACAACAGAACAACTGGCATGTGCTGCTTGATGCTGGATCTTTAGGTCCTAAAGACATGGATTCGCTTGGACTGTCCCTTTTCCGGCCAGATTTCATTGTTACATCGTTCTATAGAGTTTTTGGATTTGATCCAACCGGTTTTGGATGTCTACTAATCAAGAAATCAGTAATGGCAAGTTTACAGAATCATTCTGGGAGCACAGGATCAGGAATGGTGAAGATAACTCCTGAATATCCTTTATACCTGAGTGATTCAGCTGACGGTCTTGATGGATTCGCTGGGATTGAAGATGATGGAGTCTCTGAGAATGTTGATAAAGCCTCTGAAACACGTCAAAAATCTCAGCTGCCTGCTTTCTCGGGTGCGTTTACATCTTCTCAGGTTAGAGAGGTATTTGAAACTGAGATGGATAGTTCTGAGAGAGATGGTACGAGCACTATATTTGAGGAATCGGAGAGTATCTCTGTTGGAGAGGTGATGAAGAGCCCAGTATTCAGTGAAGATGAGTCATCTGATAATTCATTGTGGATTGATTTAGGCCACAGTCCACTTGGGTCTGATAATGCTGGCTTCAGCAAACATGAAATTGCCTCCCCATTGCCCCCATATTGGTTTGCATATCGGAAAAATAGGCGACAATCACCCAAACCAACTTCGAAAATATACAGCAGCCCACTTTATGATGACAAAGAAGTAAATTCTAGGCCAGGTGATGAAAGAAATATGCTATCATTTGATGCAGCTGTCATGTCAGTTTCTCAAGAGCTTGATCGTTACAAAGAGGTTGGTGGAGGTAAAGATTCAGGTATGAGCCCTGCTTCACAGAGTGGTAAAAAGTGTATAGCGCATACGGACGTGTTGGAGGTAGACAAAACAAGCAAGACACTTTCAAATGGTATATCAAGTTGTGATGTTAAGAGATCTCATCTTGACAATTCAACTTCTCTATCCCAGCACCATAACTTAGAGAACGGTTCGACCTCTGAGATCTGCTCAGAGATTAAGGAGAGTGCAATAAGACGTGAAACTGAAGGTGAATTCAGGTTGCTGGGATGCCGAGGGAATAGATTTGCCGGTGGGAGGTTTTTTGGAGTAGATGAATCTGAAGTGCAGAACAAGGGAAGAAGAGTCTCTTTTGGCATTGAAGACAATGGTAAAGAGCACCAGAGCTGTAATTTGGAGCCAGGTGAAACGTCTGTTACTAGCTTTGATGATGATGAAGGTACCAGTGACGGTGAATATGGTGATGGAGAAGATTGGGACAGGAGAGAACCTGAGATTATATGCCGCCATATTGATCATATCGACTTGTTGGGTCTCAATAAGACTACTCTCAGATTAAGATTTCTGATCAATTGGCTCGTGACGTCACTACTACAACTTAGGTTACCCGATTCTGATGGAAGCAGCAGAGCAAATCTTGTTCAAATTTATGGCCCTAAAATAAAATACGAAAGGGGTGCTGCCGTAGCATTCAATGTGAGAGACAAAGTTAGGGGGCTAATCAACCCAGAAATTGTACAGAAGCTGGCTGAAAGAGAGGGTATATCTCTAGGAATAGGTTTTCTTAGTCACATACGTATCGTCGACAATCCAAGACATCATAAAGGTTTGAACCTAGATGACACCACCCTTTGCAGACCAATGGAAAATGGCAAGCTTGGTGGAAAGAGCGGGTTAATGCGGGTTGAAGTCGTGACAGCTTCTCTGGGATTTTTGACCAATTTCGAAGATGTTTACAGATTATGGGCTTTTGTAGCAAAGTTTCTTAATCCAACCTTTATCAAAGAGGGTGCTCTTCCTACAGTTGAAGAAGGTTCAGAAATATAA

Coding sequence (CDS)

ATGCATCTTTCACTATGGAAAAATCTGTCTCATTGCGCTGCTGCTCTATTAATGGACAAGAAGGGCCGAAGAAGAGATGGGTCTGCCACCGGACATGACTCCAAGAAAAACTCATCAATTCTTCGAAAATTGCAAGAGAACAAGCTCAGGGAAGCCCTTGAAGAAGCATCCGAAAATGGGTCTCTCTTCAAATCGCAAGATATTGATGAATCCGAATCGCTTGGCGACCAGGATGACGGCGGCGGCTTGGGCCGGTCGCGTTCGCTCGCTAGACTCCACGCGCAGCGGGAATTCCTACGCGCCACTGCCCTCGCCGCTGAGCGCACTTTCGAGTCCGAAGACGCCATTCCTGACCTCCATGAATCTTTCTCCAAGTTCCTTACTATGTACCCGAATTACCAGTCCTCCGAGAAGATCGATCAACTGAGGTCCAACGAGTATTCGCATCTCTCTTCAAAGGTATGCCTCGATTACTGTGGCTTCGGACTTTTTTCTTATGTTCAAACACTTCACTATTGGGAGTCCTCCACATTTAGCTTGTCTGAGATTACTGCAAATCTGAGCAACCATGCTCTTTATGGAGGTGCTGAAAAGGGGACTGTTGAACATGATATAAAGCTTAAGATCATGGACTATTTGAACATTCCTGAGCATGAGTATGGTCTTGTGTTTACTGTGAGTAGAGGGTCGGCTTTTAAATTGCTTGCTGATTCATATCCTTTTCATACCAACAAAAAATTGTTAACCATGTTTGATTATGAGAGCCAGTCTGTGAGTTGGATGGGTCAGTGTGCTAGAGAGAAAGGTGCAAAAGTGTATAGCGCCTGGTTCAAATGGCCATCCCTGAAACTGTGCTCCACTGATTTGAGAAAACAGATTTCGAATAAGAGGAGAAAGAAGAAGGACTGTGCTACTGGCCTTTTTGTGTTTCCAGTTCAATCTAGAGTGACAGGGGCTAAGTATTCTTATCAATGGATGGCTCTAGCACAACAGAACAACTGGCATGTGCTGCTTGATGCTGGATCTTTAGGTCCTAAAGACATGGATTCGCTTGGACTGTCCCTTTTCCGGCCAGATTTCATTGTTACATCGTTCTATAGAGTTTTTGGATTTGATCCAACCGGTTTTGGATGTCTACTAATCAAGAAATCAGTAATGGCAAGTTTACAGAATCATTCTGGGAGCACAGGATCAGGAATGGTGAAGATAACTCCTGAATATCCTTTATACCTGAGTGATTCAGCTGACGGTCTTGATGGATTCGCTGGGATTGAAGATGATGGAGTCTCTGAGAATGTTGATAAAGCCTCTGAAACACGTCAAAAATCTCAGCTGCCTGCTTTCTCGGGTGCGTTTACATCTTCTCAGGTTAGAGAGGTATTTGAAACTGAGATGGATAGTTCTGAGAGAGATGGTACGAGCACTATATTTGAGGAATCGGAGAGTATCTCTGTTGGAGAGGTGATGAAGAGCCCAGTATTCAGTGAAGATGAGTCATCTGATAATTCATTGTGGATTGATTTAGGCCACAGTCCACTTGGGTCTGATAATGCTGGCTTCAGCAAACATGAAATTGCCTCCCCATTGCCCCCATATTGGTTTGCATATCGGAAAAATAGGCGACAATCACCCAAACCAACTTCGAAAATATACAGCAGCCCACTTTATGATGACAAAGAAGTAAATTCTAGGCCAGGTGATGAAAGAAATATGCTATCATTTGATGCAGCTGTCATGTCAGTTTCTCAAGAGCTTGATCGTTACAAAGAGGTTGGTGGAGGTAAAGATTCAGGTATGAGCCCTGCTTCACAGAGTGGTAAAAAGTGTATAGCGCATACGGACGTGTTGGAGGTAGACAAAACAAGCAAGACACTTTCAAATGGTATATCAAGTTGTGATGTTAAGAGATCTCATCTTGACAATTCAACTTCTCTATCCCAGCACCATAACTTAGAGAACGGTTCGACCTCTGAGATCTGCTCAGAGATTAAGGAGAGTGCAATAAGACGTGAAACTGAAGGTGAATTCAGGTTGCTGGGATGCCGAGGGAATAGATTTGCCGGTGGGAGGTTTTTTGGAGTAGATGAATCTGAAGTGCAGAACAAGGGAAGAAGAGTCTCTTTTGGCATTGAAGACAATGGTAAAGAGCACCAGAGCTGTAATTTGGAGCCAGGTGAAACGTCTGTTACTAGCTTTGATGATGATGAAGGTACCAGTGACGGTGAATATGGTGATGGAGAAGATTGGGACAGGAGAGAACCTGAGATTATATGCCGCCATATTGATCATATCGACTTGTTGGGTCTCAATAAGACTACTCTCAGATTAAGATTTCTGATCAATTGGCTCGTGACGTCACTACTACAACTTAGGTTACCCGATTCTGATGGAAGCAGCAGAGCAAATCTTGTTCAAATTTATGGCCCTAAAATAAAATACGAAAGGGGTGCTGCCGTAGCATTCAATGTGAGAGACAAAGTTAGGGGGCTAATCAACCCAGAAATTGTACAGAAGCTGGCTGAAAGAGAGGGTATATCTCTAGGAATAGGTTTTCTTAGTCACATACGTATCGTCGACAATCCAAGACATCATAAAGGTTTGAACCTAGATGACACCACCCTTTGCAGACCAATGGAAAATGGCAAGCTTGGTGGAAAGAGCGGGTTAATGCGGGTTGAAGTCGTGACAGCTTCTCTGGGATTTTTGACCAATTTCGAAGATGTTTACAGATTATGGGCTTTTGTAGCAAAGTTTCTTAATCCAACCTTTATCAAAGAGGGTGCTCTTCCTACAGTTGAAGAAGGTTCAGAAATATAA

Protein sequence

MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENGSLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLHESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSLSEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYPFHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKKKDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDGFAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNRRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSGMSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSEICSEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSCNLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLAEREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI
Homology
BLAST of Lsi01G010120 vs. ExPASy Swiss-Prot
Match: Q8LGM7 (Molybdenum cofactor sulfurase OS=Solanum lycopersicum OX=4081 GN=FLACCA PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 9.8e-18
Identity = 79/309 (25.57%), Postives = 139/309 (44.98%), Query Frame = 0

Query: 121 ESFSKFLTMYPNYQSSEK-IDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFS 180
           E F K    Y  Y +S K ID++R+ E+  L+  V LD+ G  L+S  Q    ++    +
Sbjct: 8   EQFLKEFGSYYGYANSPKNIDEIRATEFKRLNDTVYLDHAGATLYSESQMEAVFKDLNST 67

Query: 181 L-----SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKL 240
           L     S+ T +L+   + G A +         +++ + N    EY  +FT    +A KL
Sbjct: 68  LYGNPHSQSTCSLATEDIVGKARQ---------QVLSFFNASPREYSCIFTSGATAALKL 127

Query: 241 LADSYPFHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWF--------KWPSLKLCS 300
           + +++P+ +N   +   +    SV  + + A  KGA  ++           + P   L  
Sbjct: 128 VGETFPWSSNSSFMYSME-NHNSVLGIREYALSKGAAAFAVDIEDTHVGESESPQSNLKL 187

Query: 301 TDLRKQISNKRRKKKDCATG----LFVFPVQSRVTGAKYSYQWMALAQQNN--------- 360
           T    Q  N+    K+  TG    LF FP +   +G K+    + + ++ +         
Sbjct: 188 TQHHIQRRNEGGVLKEGMTGNTYNLFAFPSECNFSGRKFDPNLIKIIKEGSERILESSQY 247

Query: 361 ----WHVLLDAGSLGPKDMDSLGLSLFRPDFIVTSFYRVFGFDPTGFGCLLIKKSVMASL 399
               W VL+DA      +  +  LS+F+ DF+V SFY++FG+ PTG G L+++K     +
Sbjct: 248 SRGCWLVLIDAAKGCATNPPN--LSMFKADFVVFSFYKLFGY-PTGLGALIVRKDAAKLM 303

BLAST of Lsi01G010120 vs. ExPASy Swiss-Prot
Match: Q96EN8 (Molybdenum cofactor sulfurase OS=Homo sapiens OX=9606 GN=MOCOS PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 2.7e-15
Identity = 76/255 (29.80%), Postives = 117/255 (45.88%), Query Frame = 0

Query: 141 QLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSLSEIT-ANLSNHALYGGAEKG 200
           +LR+ E+S L+  V LD+ G  LFS  Q     ES T  L E T  N  +  +       
Sbjct: 37  ELRAREFSRLAGTVYLDHAGATLFSQSQL----ESFTSDLMENTYGNPHSQNISSKLTHD 96

Query: 201 TVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYPFHTN--KKLLTMFDYESQS 260
           TVE  ++ +I+ + +    +Y ++FT    +A KL+A+++P+ +   +   + F Y + S
Sbjct: 97  TVE-QVRYRILAHFHTTAEDYTVIFTAGSTAALKLVAEAFPWVSQGPESSGSRFCYLTDS 156

Query: 261 -VSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKKKDC-ATGLFVFPVQSR 320
             S +G         V S   + P     + +     SN      DC    LF +P QS 
Sbjct: 157 HTSVVGMRNVTMAINVISTPVR-PEDLWSAEERSASASN-----PDCQLPHLFCYPAQSN 216

Query: 321 VTGAKYSYQWM---------ALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIVTSFY 380
            +G +Y   W+          ++    W VLLDA S        L LS  + DF+  SFY
Sbjct: 217 FSGVRYPLSWIEEVKSGRLHPVSTPGKWFVLLDAASY--VSTSPLDLSAHQADFVPISFY 276

Query: 381 RVFGFDPTGFGCLLI 382
           ++FGF PTG G LL+
Sbjct: 277 KIFGF-PTGLGALLV 277

BLAST of Lsi01G010120 vs. ExPASy Swiss-Prot
Match: B0Y691 (Molybdenum cofactor sulfurase OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=hxB PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-14
Identity = 76/278 (27.34%), Postives = 125/278 (44.96%), Query Frame = 0

Query: 136 SEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSLSEITANL--SNHALY 195
           SE +D +R  EY  L     LD+ G  L  Y ++L      +FS  E+T+NL  + H+L 
Sbjct: 19  SEDVDTIREREYPQLKDTTYLDHAGTTL--YAKSL----IESFS-RELTSNLFGNPHSLS 78

Query: 196 GGAEKGTVE-HDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYPFHTNKKLLTMFD 255
             ++  T    D++L+ + +      E+ LVF  +  +A KL+AD     T +     + 
Sbjct: 79  TSSQLSTQRVDDVRLRALRFFKADPEEFDLVFVANATAAIKLVADGMRDSTRQGFWYGYH 138

Query: 256 YESQSVSWMGQCAREKGAKVYSAWFK---WPSLKLCSTDLRKQISNKRRKKKDCATGLFV 315
            ++ +     +   EKG + +++  +   W S +LC  D+R +           +  LF 
Sbjct: 139 VDAHTSLVGVRELAEKGGRCFTSDDEVEDWIS-RLC--DVRSE-----------SLKLFA 198

Query: 316 FPVQSRVTGAKYSYQWMALAQQ------NNWHVLLDAGSLGPKDMDSLGLSLFRPDFIVT 375
           +P QS + G +  + W    +        N + LLDA SL       L  +   PDF V 
Sbjct: 199 YPAQSNMNGRRLPFSWCKKIRDQGETTGGNVYTLLDAASLVSTSPLDLSDASAAPDFTVL 258

Query: 376 SFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMV 402
           SFY++FGF     G L+++KS    +  H    G G V
Sbjct: 259 SFYKIFGF--PDLGALIVRKSA-GQIFEHRRYFGGGTV 272

BLAST of Lsi01G010120 vs. ExPASy Swiss-Prot
Match: Q4WPE6 (Molybdenum cofactor sulfurase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=hxB PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-14
Identity = 76/278 (27.34%), Postives = 125/278 (44.96%), Query Frame = 0

Query: 136 SEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSLSEITANL--SNHALY 195
           SE +D +R  EY  L     LD+ G  L  Y ++L      +FS  E+T+NL  + H+L 
Sbjct: 19  SEDVDTIREREYPQLKDTTYLDHAGTTL--YAKSL----IESFS-RELTSNLFGNPHSLS 78

Query: 196 GGAEKGTVE-HDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYPFHTNKKLLTMFD 255
             ++  T    D++L+ + +      E+ LVF  +  +A KL+AD     T +     + 
Sbjct: 79  TSSQLSTQRVDDVRLRALRFFKADPEEFDLVFVANATAAIKLVADGMRDSTRQGFWYGYH 138

Query: 256 YESQSVSWMGQCAREKGAKVYSAWFK---WPSLKLCSTDLRKQISNKRRKKKDCATGLFV 315
            ++ +     +   EKG + +++  +   W S +LC  D+R +           +  LF 
Sbjct: 139 VDAHTSLVGVRELAEKGGRCFTSDDEVEDWIS-RLC--DVRSE-----------SLKLFA 198

Query: 316 FPVQSRVTGAKYSYQWMALAQQ------NNWHVLLDAGSLGPKDMDSLGLSLFRPDFIVT 375
           +P QS + G +  + W    +        N + LLDA SL       L  +   PDF V 
Sbjct: 199 YPAQSNMNGRRLPFSWCKKIRDQGETTGGNVYTLLDAASLVSTSPLDLSDASAAPDFTVL 258

Query: 376 SFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMV 402
           SFY++FGF     G L+++KS    +  H    G G V
Sbjct: 259 SFYKIFGF--PDLGALIVRKSA-GQIFEHRRYFGGGTV 272

BLAST of Lsi01G010120 vs. ExPASy Swiss-Prot
Match: Q7QFL7 (Molybdenum cofactor sulfurase OS=Anopheles gambiae OX=7165 GN=mal PE=3 SV=5)

HSP 1 Score: 81.3 bits (199), Expect = 6.6e-14
Identity = 79/296 (26.69%), Postives = 123/296 (41.55%), Query Frame = 0

Query: 138 KIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSLSEITANLSNHALYGGAE 197
           KI+Q    ++S L+ K  LD+ G  L        Y ES   ++ E+ A      LY    
Sbjct: 14  KIEQ----DFSRLADKCYLDHAGTAL--------YGESQLRAVQELLAG----GLYCNPH 73

Query: 198 KGTVEHD----IKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYPFHTNKKLLTMFDY 257
                 D    ++ +++ +      +Y LVFT    ++ KL+A+S+ F         F Y
Sbjct: 74  TSRTMEDLIDLVRYRVLRWFQTRPADYSLVFTSGTTASLKLVAESFEFGPGDAEPGSFVY 133

Query: 258 ESQS-VSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKR--RKKKDCATGLFVF 317
              S  S +G     +  +V           +   +L + ++     R++      L VF
Sbjct: 134 LRDSHTSVLGMRELVRTGRVQ---------PIERAELLQALNEPEDPRRQHPHRPSLLVF 193

Query: 318 PVQSRVTGAKYSYQWMALAQQNN--------WHVLLDAGSLGPKDMDSLGLSLFRPDFIV 377
           P Q    GAKY  +   L ++N         +HV LDA S        L LS +RP F+ 
Sbjct: 194 PAQCNFNGAKYPLELCELIERNGLRGYGGDAFHVCLDAAS--HVSTSPLDLSRYRPSFVC 253

Query: 378 TSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGL 419
            SFY++FG+ PTG G LL+++     L+      G G VKI    P    +  D L
Sbjct: 254 LSFYKIFGY-PTGLGALLVRRDAEPLLRG-KRYYGGGTVKIALSGPDRFHERRDAL 280

BLAST of Lsi01G010120 vs. ExPASy TrEMBL
Match: A0A5D3CRF4 (Pyridoxal phosphate-dependent transferases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005140 PE=4 SV=1)

HSP 1 Score: 1815.8 bits (4702), Expect = 0.0e+00
Identity = 911/938 (97.12%), Postives = 924/938 (98.51%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGS TGHDSKKNSSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSTTGHDSKKNSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFES+DAIPDLH
Sbjct: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESDDAIPDLH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           E+FSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 EAFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSVDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES 480
           FAGIE+DGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERD TSTIFEES
Sbjct: 421 FAGIEEDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDATSTIFEES 480

Query: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR 540
           ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKN 
Sbjct: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNN 540

Query: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSGMS 600
           RQSPKPTSKIYSSPLYD+KEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDS MS
Sbjct: 541 RQSPKPTSKIYSSPLYDEKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSVMS 600

Query: 601 PASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSEIC 660
           PAS SGKK + HTD+LEVDKTSK LSNG+SSCDVK+SHL NSTS SQHHNLENGSTSEIC
Sbjct: 601 PASLSGKKSLEHTDMLEVDKTSKRLSNGLSSCDVKKSHLGNSTSGSQHHNLENGSTSEIC 660

Query: 661 SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720
           SEIKESAIRRETEGEFRLLG RGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC
Sbjct: 661 SEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720

Query: 721 NLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780
           NLEPGETSVTSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI
Sbjct: 721 NLEPGETSVTSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780

Query: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840
           NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQ+LA
Sbjct: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQRLA 840

Query: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLG 900
           EREGISLGIGFLSHIRIV+NPRHHKGLNL+DTTLCRPMENGKL GKSG MRVEVVTASLG
Sbjct: 841 EREGISLGIGFLSHIRIVENPRHHKGLNLEDTTLCRPMENGKLSGKSGFMRVEVVTASLG 900

Query: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 938

BLAST of Lsi01G010120 vs. ExPASy TrEMBL
Match: A0A1S3B185 (uncharacterized protein LOC103484742 OS=Cucumis melo OX=3656 GN=LOC103484742 PE=4 SV=1)

HSP 1 Score: 1815.8 bits (4702), Expect = 0.0e+00
Identity = 911/938 (97.12%), Postives = 924/938 (98.51%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGS TGHDSKKNSSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSTTGHDSKKNSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFES+DAIPDLH
Sbjct: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESDDAIPDLH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           E+FSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 EAFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSVDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES 480
           FAGIE+DGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERD TSTIFEES
Sbjct: 421 FAGIEEDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDATSTIFEES 480

Query: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR 540
           ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKN 
Sbjct: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNN 540

Query: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSGMS 600
           RQSPKPTSKIYSSPLYD+KEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDS MS
Sbjct: 541 RQSPKPTSKIYSSPLYDEKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSVMS 600

Query: 601 PASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSEIC 660
           PAS SGKK + HTD+LEVDKTSK LSNG+SSCDVK+SHL NSTS SQHHNLENGSTSEIC
Sbjct: 601 PASLSGKKSLEHTDMLEVDKTSKRLSNGLSSCDVKKSHLGNSTSGSQHHNLENGSTSEIC 660

Query: 661 SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720
           SEIKESAIRRETEGEFRLLG RGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC
Sbjct: 661 SEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720

Query: 721 NLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780
           NLEPGETSVTSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI
Sbjct: 721 NLEPGETSVTSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780

Query: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840
           NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQ+LA
Sbjct: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQRLA 840

Query: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLG 900
           EREGISLGIGFLSHIRIV+NPRHHKGLNL+DTTLCRPMENGKL GKSG MRVEVVTASLG
Sbjct: 841 EREGISLGIGFLSHIRIVENPRHHKGLNLEDTTLCRPMENGKLSGKSGFMRVEVVTASLG 900

Query: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 938

BLAST of Lsi01G010120 vs. ExPASy TrEMBL
Match: A0A0A0KI55 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490000 PE=4 SV=1)

HSP 1 Score: 1815.0 bits (4700), Expect = 0.0e+00
Identity = 913/938 (97.33%), Postives = 922/938 (98.29%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDG ATGHDSKKNSSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGPATGHDSKKNSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIP+LH
Sbjct: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPELH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQN+SGSTGSGMVKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNNSGSTGSGMVKITPEYPLYLSDSVDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES 480
           F GIE+DGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERD TSTIFEES
Sbjct: 421 FTGIEEDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDATSTIFEES 480

Query: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR 540
           ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSD AGFSKHEIASPLPPYWFAYRKN 
Sbjct: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDTAGFSKHEIASPLPPYWFAYRKNS 540

Query: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSGMS 600
           RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDS MS
Sbjct: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSVMS 600

Query: 601 PASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSEIC 660
           PAS SGKK + HTD+LEVDKTSK LSNG+SSCDVK+SHL NSTS SQHHNLENGSTSEIC
Sbjct: 601 PASLSGKKSLEHTDMLEVDKTSKRLSNGLSSCDVKKSHLGNSTSGSQHHNLENGSTSEIC 660

Query: 661 SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720
           SEIKESAIRRETEGEFRLLG RGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC
Sbjct: 661 SEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720

Query: 721 NLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780
           NLEPGETSVTSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI
Sbjct: 721 NLEPGETSVTSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780

Query: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840
           NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA
Sbjct: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840

Query: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLG 900
           EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKL GKSG MRVEVVTASLG
Sbjct: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLSGKSGFMRVEVVTASLG 900

Query: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 938

BLAST of Lsi01G010120 vs. ExPASy TrEMBL
Match: A0A6J1IJ46 (uncharacterized protein LOC111477906 OS=Cucurbita maxima OX=3661 GN=LOC111477906 PE=4 SV=1)

HSP 1 Score: 1738.4 bits (4501), Expect = 0.0e+00
Identity = 878/941 (93.30%), Postives = 901/941 (95.75%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHD+KK+SSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDAKKSSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDES+SLGDQDDG GLGRSRSLARLHAQREFLRATALAAERT+ESED IPD H
Sbjct: 61  SLFKSQDIDESDSLGDQDDGSGLGRSRSLARLHAQREFLRATALAAERTYESEDDIPDFH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           E FSKF TMYPN+QSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 ECFSKFFTMYPNFQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSV+WM Q AREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVTWMAQSAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KD ATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDSATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSG+VKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGIVKITPEYPLYLSDSIDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMD--SSERDGTSTIFE 480
           FAGIE+DG+SENV KASETRQKSQLPAFSGAFTSSQVR+VFETEMD  SSERD TSTIFE
Sbjct: 421 FAGIEEDGISENVVKASETRQKSQLPAFSGAFTSSQVRDVFETEMDHESSERDATSTIFE 480

Query: 481 ESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540
           E+ES SVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK
Sbjct: 481 ETESFSVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540

Query: 541 NRRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSG 600
           NR+QSPKPTSKIYSSPLYDDKEVNSR GDER+MLSFDAAVMSVSQELDRYKEVGG KDS 
Sbjct: 541 NRQQSPKPTSKIYSSPLYDDKEVNSRAGDERSMLSFDAAVMSVSQELDRYKEVGGSKDSV 600

Query: 601 MSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSE 660
            SP  Q GKK   HTDVLEVDK  K LSNGISSCDVKRSHL NSTS SQHHNLENGSTSE
Sbjct: 601 RSPVLQGGKKSSEHTDVLEVDKIGKALSNGISSCDVKRSHLSNSTSGSQHHNLENGSTSE 660

Query: 661 ICSEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQ 720
           +C EIKESAIRRETEGEFRLLG RGNRFAGGRFFGV+ESEVQNKGRRVSFGIEDNGKEHQ
Sbjct: 661 VCREIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVEESEVQNKGRRVSFGIEDNGKEHQ 720

Query: 721 SCNLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRF 780
           SCN EPGE S+TSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLR+
Sbjct: 721 SCNFEPGEMSITSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRY 780

Query: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQK 840
           LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRD+VRGLINPEIVQK
Sbjct: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDRVRGLINPEIVQK 840

Query: 841 LAEREGISLGIGFLSHIRIVDNPRHHK-GLNLDDTTLCRPMENGKLGGKSGLMRVEVVTA 900
           LAEREGISLGIGFLSHIRIVDNPRH K G NL+DTTLCRPME+GKL GKS  MR EVVTA
Sbjct: 841 LAEREGISLGIGFLSHIRIVDNPRHQKSGSNLEDTTLCRPMESGKLSGKSEFMRGEVVTA 900

Query: 901 SLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           SLGFL+NFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 SLGFLSNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 941

BLAST of Lsi01G010120 vs. ExPASy TrEMBL
Match: A0A6J1GE67 (uncharacterized protein LOC111453345 OS=Cucurbita moschata OX=3662 GN=LOC111453345 PE=4 SV=1)

HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 878/941 (93.30%), Postives = 901/941 (95.75%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHD+KK+SSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDAKKSSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDES+SLGDQDDG GLGRSRSLARLHAQREFLRATALAAERT+ESED IPDLH
Sbjct: 61  SLFKSQDIDESDSLGDQDDGSGLGRSRSLARLHAQREFLRATALAAERTYESEDDIPDLH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           E FSKF TMYPN+QSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 ECFSKFFTMYPNFQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSV+WM Q AREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVTWMAQSAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KD ATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDSATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSG+VKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGIVKITPEYPLYLSDSIDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMD--SSERDGTSTIFE 480
           FAGIE+DG+SENV KASETRQKSQLPAFSGAFTSSQVR+VFETEMD  SSERD TSTIFE
Sbjct: 421 FAGIEEDGISENVVKASETRQKSQLPAFSGAFTSSQVRDVFETEMDHESSERDATSTIFE 480

Query: 481 ESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540
           E+ES SVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK
Sbjct: 481 ETESFSVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540

Query: 541 NRRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSG 600
           NR+QSPKPTSKIYSSPLYDDKEVNSR GDER+MLSFDAAVMSVSQELDRYKEVGG KDS 
Sbjct: 541 NRQQSPKPTSKIYSSPLYDDKEVNSRAGDERSMLSFDAAVMSVSQELDRYKEVGGSKDSV 600

Query: 601 MSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSE 660
            SP  Q GKK   HTDVLEVDK  K LSNG+SSCDVKRSHL NSTS SQHH LENGSTSE
Sbjct: 601 RSPVVQGGKKSSEHTDVLEVDKIGKALSNGMSSCDVKRSHLSNSTSGSQHHKLENGSTSE 660

Query: 661 ICSEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQ 720
           IC EIKESAIRRETEGEFRLLG RGNRFAGGRFFGV+ESEVQNKGRRVSFGIEDNGKEHQ
Sbjct: 661 ICPEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVEESEVQNKGRRVSFGIEDNGKEHQ 720

Query: 721 SCNLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRF 780
           SCN EPGE S+TSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLR+
Sbjct: 721 SCNFEPGEMSITSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRY 780

Query: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQK 840
           LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRD+VRGLINPEIVQK
Sbjct: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDRVRGLINPEIVQK 840

Query: 841 LAEREGISLGIGFLSHIRIVDNPRHHK-GLNLDDTTLCRPMENGKLGGKSGLMRVEVVTA 900
           LAEREGISLGIGFLSHIRIVDNPRH K G NL+DTTLCRPME+GKL GKS  MR EVVTA
Sbjct: 841 LAEREGISLGIGFLSHIRIVDNPRHQKSGSNLEDTTLCRPMESGKLSGKSEFMRGEVVTA 900

Query: 901 SLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           SLGFL+NFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 SLGFLSNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 941

BLAST of Lsi01G010120 vs. NCBI nr
Match: XP_038881711.1 (uncharacterized protein LOC120073139 [Benincasa hispida])

HSP 1 Score: 1825.4 bits (4727), Expect = 0.0e+00
Identity = 916/938 (97.65%), Postives = 927/938 (98.83%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH
Sbjct: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES 480
           FAGIE DG SENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES
Sbjct: 421 FAGIEGDGDSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES 480

Query: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR 540
           ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR
Sbjct: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR 540

Query: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSGMS 600
           RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDS MS
Sbjct: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSVMS 600

Query: 601 PASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSEIC 660
           PAS+SGKK + HT+++EVDKTSKTLSNG+S CDVKRSHL NSTS SQ HNLENGSTSEIC
Sbjct: 601 PASRSGKKSLEHTNLMEVDKTSKTLSNGMSGCDVKRSHLGNSTSGSQQHNLENGSTSEIC 660

Query: 661 SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720
           SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGV+ESEVQNKGRRVSFGI+DNGKEHQSC
Sbjct: 661 SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVEESEVQNKGRRVSFGIDDNGKEHQSC 720

Query: 721 NLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780
           NLEPGETSVTSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI
Sbjct: 721 NLEPGETSVTSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780

Query: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840
           NWLVTSLLQLRLPDSDGSSR NLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA
Sbjct: 781 NWLVTSLLQLRLPDSDGSSRGNLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840

Query: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLG 900
           EREGISLGIGFLSHIRIVDNPRHHKGLNL+DTTLCRPMENGKLGGKSGLMRVEVVTASLG
Sbjct: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLEDTTLCRPMENGKLGGKSGLMRVEVVTASLG 900

Query: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           FLTNFEDVYRLWAFVAKFLNPTFIKEG LPTVEEGSE+
Sbjct: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGVLPTVEEGSEV 938

BLAST of Lsi01G010120 vs. NCBI nr
Match: XP_008440224.1 (PREDICTED: uncharacterized protein LOC103484742 [Cucumis melo] >TYK12919.1 Pyridoxal phosphate-dependent transferases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 1815.8 bits (4702), Expect = 0.0e+00
Identity = 911/938 (97.12%), Postives = 924/938 (98.51%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGS TGHDSKKNSSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSTTGHDSKKNSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFES+DAIPDLH
Sbjct: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESDDAIPDLH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           E+FSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 EAFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSVDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES 480
           FAGIE+DGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERD TSTIFEES
Sbjct: 421 FAGIEEDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDATSTIFEES 480

Query: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR 540
           ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKN 
Sbjct: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNN 540

Query: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSGMS 600
           RQSPKPTSKIYSSPLYD+KEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDS MS
Sbjct: 541 RQSPKPTSKIYSSPLYDEKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSVMS 600

Query: 601 PASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSEIC 660
           PAS SGKK + HTD+LEVDKTSK LSNG+SSCDVK+SHL NSTS SQHHNLENGSTSEIC
Sbjct: 601 PASLSGKKSLEHTDMLEVDKTSKRLSNGLSSCDVKKSHLGNSTSGSQHHNLENGSTSEIC 660

Query: 661 SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720
           SEIKESAIRRETEGEFRLLG RGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC
Sbjct: 661 SEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720

Query: 721 NLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780
           NLEPGETSVTSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI
Sbjct: 721 NLEPGETSVTSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780

Query: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840
           NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQ+LA
Sbjct: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQRLA 840

Query: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLG 900
           EREGISLGIGFLSHIRIV+NPRHHKGLNL+DTTLCRPMENGKL GKSG MRVEVVTASLG
Sbjct: 841 EREGISLGIGFLSHIRIVENPRHHKGLNLEDTTLCRPMENGKLSGKSGFMRVEVVTASLG 900

Query: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 938

BLAST of Lsi01G010120 vs. NCBI nr
Match: XP_004141937.1 (uncharacterized protein LOC101221457 [Cucumis sativus] >KGN48484.1 hypothetical protein Csa_003819 [Cucumis sativus])

HSP 1 Score: 1815.0 bits (4700), Expect = 0.0e+00
Identity = 913/938 (97.33%), Postives = 922/938 (98.29%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDG ATGHDSKKNSSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGPATGHDSKKNSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIP+LH
Sbjct: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPELH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQN+SGSTGSGMVKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNNSGSTGSGMVKITPEYPLYLSDSVDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDGTSTIFEES 480
           F GIE+DGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERD TSTIFEES
Sbjct: 421 FTGIEEDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMDSSERDATSTIFEES 480

Query: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRKNR 540
           ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSD AGFSKHEIASPLPPYWFAYRKN 
Sbjct: 481 ESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDTAGFSKHEIASPLPPYWFAYRKNS 540

Query: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSGMS 600
           RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDS MS
Sbjct: 541 RQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSVMS 600

Query: 601 PASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSEIC 660
           PAS SGKK + HTD+LEVDKTSK LSNG+SSCDVK+SHL NSTS SQHHNLENGSTSEIC
Sbjct: 601 PASLSGKKSLEHTDMLEVDKTSKRLSNGLSSCDVKKSHLGNSTSGSQHHNLENGSTSEIC 660

Query: 661 SEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720
           SEIKESAIRRETEGEFRLLG RGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC
Sbjct: 661 SEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQSC 720

Query: 721 NLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780
           NLEPGETSVTSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI
Sbjct: 721 NLEPGETSVTSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLI 780

Query: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840
           NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA
Sbjct: 781 NWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLA 840

Query: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLG 900
           EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKL GKSG MRVEVVTASLG
Sbjct: 841 EREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLSGKSGFMRVEVVTASLG 900

Query: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 FLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 938

BLAST of Lsi01G010120 vs. NCBI nr
Match: XP_023543927.1 (uncharacterized protein LOC111803649 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1740.3 bits (4506), Expect = 0.0e+00
Identity = 880/941 (93.52%), Postives = 903/941 (95.96%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHD+KK+SSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDAKKSSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDES+SLGDQDDG GLGRSRSLARLHAQREFLRATALAAERT+ESED IPDLH
Sbjct: 61  SLFKSQDIDESDSLGDQDDGSGLGRSRSLARLHAQREFLRATALAAERTYESEDDIPDLH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           E FSKFLTMYPN+QSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 ECFSKFLTMYPNFQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSV+WM Q AREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVTWMAQSAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KD ATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDSATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSG+VKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGIVKITPEYPLYLSDSIDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMD--SSERDGTSTIFE 480
           FAGIE+DG+SENV KASETRQKSQLPAFSGAFTSSQVR+VFETEMD  SSERD TSTIFE
Sbjct: 421 FAGIEEDGISENVVKASETRQKSQLPAFSGAFTSSQVRDVFETEMDHESSERDATSTIFE 480

Query: 481 ESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540
           E+ES SVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK
Sbjct: 481 ETESFSVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540

Query: 541 NRRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSG 600
           NR+QSPKPTSKIYSSPLYDDKEVNSR GDER+MLSFDAAVMSVSQELDRYKEVGG KDS 
Sbjct: 541 NRQQSPKPTSKIYSSPLYDDKEVNSRAGDERSMLSFDAAVMSVSQELDRYKEVGGSKDSI 600

Query: 601 MSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSE 660
            SP  Q GKK   HTDVLEVDK  K LSNG+SSCDVKRSHL NSTS SQHHNLENGSTSE
Sbjct: 601 RSPVLQGGKKSSEHTDVLEVDKIGKALSNGMSSCDVKRSHLSNSTSGSQHHNLENGSTSE 660

Query: 661 ICSEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQ 720
           IC EIKESAIRRETEGEFRLLG RGNRFAGGRFFGV+ESEVQNKGRRVSFGIEDNGKEHQ
Sbjct: 661 ICPEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVEESEVQNKGRRVSFGIEDNGKEHQ 720

Query: 721 SCNLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRF 780
           SCN EPGE S+TSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLR+
Sbjct: 721 SCNFEPGEMSITSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRY 780

Query: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQK 840
           LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRD+VRGLINPEIVQK
Sbjct: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDRVRGLINPEIVQK 840

Query: 841 LAEREGISLGIGFLSHIRIVDNPRHHK-GLNLDDTTLCRPMENGKLGGKSGLMRVEVVTA 900
           LAEREGISLGIGFLSHIRIVDNPRH K G NL+DTTLCRPME+GKL GKS  MR EVVTA
Sbjct: 841 LAEREGISLGIGFLSHIRIVDNPRHQKSGSNLEDTTLCRPMESGKLSGKSEFMRGEVVTA 900

Query: 901 SLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           SLGFL+NFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 SLGFLSNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 941

BLAST of Lsi01G010120 vs. NCBI nr
Match: KAG6604205.1 (Molybdenum cofactor sulfurase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1738.4 bits (4501), Expect = 0.0e+00
Identity = 879/941 (93.41%), Postives = 902/941 (95.86%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHD+KK+SSILRKLQENKLREALEEASENG
Sbjct: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDAKKSSSILRKLQENKLREALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQDIDES+SLGDQDDG GLGRSRSLARLHAQREFLRATALAAERT+ESED IPDLH
Sbjct: 61  SLFKSQDIDESDSLGDQDDGSGLGRSRSLARLHAQREFLRATALAAERTYESEDDIPDLH 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180
           E FSKFLTMYPN+QSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL
Sbjct: 121 ECFSKFLTMYPNFQSSEKIDQLRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSL 180

Query: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240
           SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP
Sbjct: 181 SEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYP 240

Query: 241 FHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300
           FHTNKKLLTMFDYESQSV+WM Q AREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK
Sbjct: 241 FHTNKKLLTMFDYESQSVTWMAQSAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKK 300

Query: 301 KDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360
           KD ATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF
Sbjct: 301 KDSATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDF 360

Query: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLDG 420
           IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSG+VKITPEYPLYLSDS DGLDG
Sbjct: 361 IVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGIVKITPEYPLYLSDSIDGLDG 420

Query: 421 FAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMD--SSERDGTSTIFE 480
           FAGIE+DG+SENV KASETRQKSQLPAFSGAFTSSQVR+VFETEMD  SSERD TSTIFE
Sbjct: 421 FAGIEEDGISENVVKASETRQKSQLPAFSGAFTSSQVRDVFETEMDHESSERDATSTIFE 480

Query: 481 ESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540
           E+ES SVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK
Sbjct: 481 ETESFSVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFAYRK 540

Query: 541 NRRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGGGKDSG 600
           NR+QSPKPTSKIYSSPLYDDKEVNSR GDER+MLSFDAAVMSVSQELDRYKEVGG KDS 
Sbjct: 541 NRQQSPKPTSKIYSSPLYDDKEVNSRAGDERSMLSFDAAVMSVSQELDRYKEVGGSKDSV 600

Query: 601 MSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLENGSTSE 660
            SP  Q GKK   HTDVLEVDK  K LSNG+SSCDVKRSHL NSTS SQHH LENGSTSE
Sbjct: 601 RSPVVQGGKKSSEHTDVLEVDKIGKALSNGMSSCDVKRSHLSNSTSGSQHHKLENGSTSE 660

Query: 661 ICSEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIEDNGKEHQ 720
           IC EIKESAIRRETEGEFRLLG RGNRFAGGRFFGV+ESEVQNKGRRVSFGIEDNGKEHQ
Sbjct: 661 ICPEIKESAIRRETEGEFRLLGRRGNRFAGGRFFGVEESEVQNKGRRVSFGIEDNGKEHQ 720

Query: 721 SCNLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRF 780
           SCN EPGE S+TSFDDDEGTSDGEYGDG+DWDRREPEIICRHIDHIDLLGLNKTTLRLR+
Sbjct: 721 SCNFEPGEMSITSFDDDEGTSDGEYGDGQDWDRREPEIICRHIDHIDLLGLNKTTLRLRY 780

Query: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQK 840
           LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRD+VRGLINPEIVQK
Sbjct: 781 LINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDRVRGLINPEIVQK 840

Query: 841 LAEREGISLGIGFLSHIRIVDNPRHHK-GLNLDDTTLCRPMENGKLGGKSGLMRVEVVTA 900
           LAEREGISLGIGFLSHIRIVDNPRH K G NL+DTTLCRPME+GKL GKS  MR EVVTA
Sbjct: 841 LAEREGISLGIGFLSHIRIVDNPRHQKSGSNLEDTTLCRPMESGKLSGKSEFMRGEVVTA 900

Query: 901 SLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEI 939
           SLGFL+NFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSE+
Sbjct: 901 SLGFLSNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEGSEV 941

BLAST of Lsi01G010120 vs. TAIR 10
Match: AT2G23520.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 622/948 (65.61%), Postives = 745/948 (78.59%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSILRKLQENKLREALEEASENG 60
           MH  LWK + HCA  +L   K RRRDGS +  D ++ +S+LRKL E+KLR+ALEEASENG
Sbjct: 1   MHFPLWKQIHHCATLILDKSKSRRRDGSDSPIDVRRKASMLRKLYEDKLRDALEEASENG 60

Query: 61  SLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDLH 120
           SLFKSQD++      +QD+   LGRSRSLARLHAQREFLRATALAAER FESED IP+L 
Sbjct: 61  SLFKSQDVENE----NQDE--SLGRSRSLARLHAQREFLRATALAAERAFESEDDIPELL 120

Query: 121 ESFSKFLTMYPNYQSSEKIDQLRSNEYSH-LSSKVCLDYCGFGLFSYVQTLHYWESSTFS 180
           E+F+KFLTMYP +++SEK+DQLRS+EY H L SKVCLDYCGFGLFSYVQTLHYW+S TFS
Sbjct: 121 EAFNKFLTMYPKFETSEKVDQLRSDEYGHLLDSKVCLDYCGFGLFSYVQTLHYWDSCTFS 180

Query: 181 LSEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSY 240
           LSEITANLSNHALYGGAE GTVEHD+K +IMDYLNIPE EYGLVFT SRGSAF+LLA+SY
Sbjct: 181 LSEITANLSNHALYGGAEIGTVEHDLKTRIMDYLNIPESEYGLVFTGSRGSAFRLLAESY 240

Query: 241 PFHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRK 300
           PFHTNK+LLTMFD+ESQSV+WM Q AREKGAK Y+AWFKWP+LKLCSTDL+K++S+K+RK
Sbjct: 241 PFHTNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTDLKKRLSHKKRK 300

Query: 301 KKDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPD 360
           KKD A GLFVFP QSRVTG+KYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRP+
Sbjct: 301 KKDSAVGLFVFPAQSRVTGSKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPE 360

Query: 361 FIVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGLD 420
           FI+TSFY+VFG DPTGFGCLLIKKSVM +LQ+ SG TGSG+VKITP+YPLYLSDS DGLD
Sbjct: 361 FIITSFYKVFGHDPTGFGCLLIKKSVMGNLQSQSGKTGSGIVKITPQYPLYLSDSIDGLD 420

Query: 421 GFAGIEDDGVSENVDKASET---RQKSQLPAFSGAFTSSQVREVFETEM---DSSERDGT 480
           G  G+ED  +  N DK + T   R+ +Q+P FSGA+TS+QVR+VFET++   ++S+RDGT
Sbjct: 421 GLVGLEDHDIGTNGDKPATTDAARRGAQMPVFSGAYTSAQVRDVFETDLLEDNASDRDGT 480

Query: 481 -STIFEESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAG-FSKHEIASPLPP 540
            STIFEE+ES+SVGE+MKSP FSEDESSDNS WIDLG SPLGSD+AG  + H+IASPLPP
Sbjct: 481 SSTIFEENESVSVGELMKSPAFSEDESSDNSFWIDLGQSPLGSDSAGHLNHHKIASPLPP 540

Query: 541 YWFAYRKNRRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEV 600
           +WF    ++RQSPKP +K YSSP+YD K+V          LSFDAAVMSV+QE++     
Sbjct: 541 FWFT---SKRQSPKPVAKSYSSPMYDGKDV----------LSFDAAVMSVTQEIN----- 600

Query: 601 GGGKDSGMSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNL 660
                   S  S++ +                  SN +   +++  +  N    +     
Sbjct: 601 --------STPSRNLRN-----------------SNNLQIQEIQEENCGNIVYRAGSGFG 660

Query: 661 ENGSTSEICSEIKESAIRRETEGEFRLLGCRGNRFAGGRFFGVDESEVQNKGRRVSFGIE 720
            NGS+S+I S++K++AIRRETEGEFRLLG RG    GGR  G+ E E  ++G RVSF + 
Sbjct: 661 SNGSSSKISSDMKDNAIRRETEGEFRLLGRRG---TGGRLLGL-EDEQPSRGTRVSFNM- 720

Query: 721 DNGKEHQSCNLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNK 780
               +  S +L+ GE S+ S  D+   SDGE  + +DWDRREPEI+C HIDH+++LGLNK
Sbjct: 721 ----DRVSHSLDQGEASLASVYDE---SDGENPNEDDWDRREPEIVCSHIDHVNMLGLNK 780

Query: 781 TTLRLRFLINWLVTSLLQLRLPD--SDGSSR-ANLVQIYGPKIKYERGAAVAFNVRDKVR 840
           TT RLRFLINWLV SLLQL++P+  SDGSSR  NLVQIYGPKIKYERGAAVAFNV+DK +
Sbjct: 781 TTSRLRFLINWLVISLLQLKVPEPGSDGSSRYMNLVQIYGPKIKYERGAAVAFNVKDKSK 840

Query: 841 GLINPEIVQKLAEREGISLGIGFLSHIRIVDNPRHHKG--LNLDDTTLCRPMENGKLGGK 900
           G ++PEIV KLAEREG+SLGIG LSHIRI+D PR+H+G     +D++L    E GK GGK
Sbjct: 841 GFVSPEIVLKLAEREGVSLGIGILSHIRIMDLPRNHRGGARIKEDSSLHLQREAGKRGGK 887

Query: 901 SGLMRVEVVTASLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEE 935
           +G +R EVVTASL FL+NFEDVY+LWAFVAKFLNP F +EG+LPTV E
Sbjct: 901 NGFVRFEVVTASLSFLSNFEDVYKLWAFVAKFLNPGFSREGSLPTVIE 887

BLAST of Lsi01G010120 vs. TAIR 10
Match: AT4G37100.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 630/962 (65.49%), Postives = 726/962 (75.47%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKG--RRRDGSATGHDSKKNSSILRKLQENKLREALEEASE 60
           MH SLWK + HCA+ +L   K   RRRDGS +  + KK ++++RKL E+KLREALEEASE
Sbjct: 1   MHFSLWKQIHHCASLILDKSKSSRRRRDGSDSSLNVKKKAALIRKLYEDKLREALEEASE 60

Query: 61  NGSLFKSQDIDESESLGDQDDG-GGLGRSRSLARLHAQREFLRATALAAERTFESEDAIP 120
           NGSLFKSQDI       DQD+G G LGRSRSLARLHAQREFLRATALAAER  ESED+IP
Sbjct: 61  NGSLFKSQDI-------DQDNGDGSLGRSRSLARLHAQREFLRATALAAERIIESEDSIP 120

Query: 121 DLHESFSKFLTMYPNYQSSEKIDQLRSNEYSHLS---SKVCLDYCGFGLFSYVQTLHYWE 180
           +L E+ +KFL+MYP YQ+SEKIDQLRS+EYSHLS   SKVCLDYCGFGLFSYVQTLHYW+
Sbjct: 121 ELREALTKFLSMYPKYQASEKIDQLRSDEYSHLSSSASKVCLDYCGFGLFSYVQTLHYWD 180

Query: 181 SSTFSLSEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKL 240
           + TFSLSEITANLSNHALYGGAE GTVEHDIK +IMDYLNIPE+EYGLVFTVSRGSAF+L
Sbjct: 181 TCTFSLSEITANLSNHALYGGAESGTVEHDIKTRIMDYLNIPENEYGLVFTVSRGSAFRL 240

Query: 241 LADSYPFHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQIS 300
           LA+SYPF +NK+LLTMFD+ESQSV+WM Q AREKGAK Y+AWFKWP+LKLCSTDL+K++S
Sbjct: 241 LAESYPFQSNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTDLKKRLS 300

Query: 301 NKRRKKKDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLS 360
            K+RKKKD A GLFVFP QSRVTG KYSYQWMALAQQN+WHVLLDAGSLGPKDMDSLGLS
Sbjct: 301 YKKRKKKDSAVGLFVFPAQSRVTGTKYSYQWMALAQQNHWHVLLDAGSLGPKDMDSLGLS 360

Query: 361 LFRPDFIVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDS 420
           LFRP+FI+TSFYRVFG DPTGFGCLLIKKSVM SLQ+ SG TGSG+VKITPEYPLYLSDS
Sbjct: 361 LFRPEFIITSFYRVFGHDPTGFGCLLIKKSVMGSLQSQSGKTGSGIVKITPEYPLYLSDS 420

Query: 421 ADGLDGFAGIEDDGVSENVDKASET-RQKSQLPAFSGAFTSSQVREVFETEM----DSSE 480
            DGLDG  G ED     N DK  E  R  +Q+PAFSGA+TS+QVR+VFETE+     SS+
Sbjct: 421 VDGLDGLVGFED----HNDDKTKEAHRPGTQMPAFSGAYTSAQVRDVFETELLEDNISSD 480

Query: 481 RDGT--STIFEESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIAS 540
           RDGT  +TIFEE+ES+SVGE+MKSPVFSEDESSDNS WIDLG SPLGSD      ++IAS
Sbjct: 481 RDGTTSTTIFEETESVSVGELMKSPVFSEDESSDNSFWIDLGQSPLGSD----QHNKIAS 540

Query: 541 PLPPYWFAYRK--NRRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQEL 600
           PLPP W   ++   +RQSPKP  K YSSPLYD  +V          LSFDAAVMSV++  
Sbjct: 541 PLPPIWLTNKRKQKQRQSPKPIPKSYSSPLYDGNDV----------LSFDAAVMSVTEH- 600

Query: 601 DRYKEVGGGKDSGMSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSL 660
                   G +S  S   +S    + H   ++ +    + +NG+ S              
Sbjct: 601 --------GTNSTPSRNRRSSSNHL-HVQEIQEENCGHSFANGLKS-------------- 660

Query: 661 SQHHNLENGSTSEICSEIKESAIRRETEGEFRLLGCR-GNRFAGGRFFGVDESEVQNKGR 720
                      S I SEIKESAIRRETEGEFRLLG R G R    R  GV E E  +KGR
Sbjct: 661 -----------SNISSEIKESAIRRETEGEFRLLGGRDGGR---SRLLGV-EDEHPSKGR 720

Query: 721 RVSFGIEDNGKEHQSCNLEPGETSVTSFDDDE--GTSDGEYGDGE----DWDRR--EPEI 780
           RVSF +E          +EPGE S+ S  D++   TSD E GD E    +WDRR  E EI
Sbjct: 721 RVSFNME----RVSHSIVEPGEASLASVYDEDYINTSDVENGDDEGADDEWDRRDTETEI 780

Query: 781 ICRHIDHIDLLGLNKTTLRLRFLINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERG 840
           +CRHIDH+++LGLNKTT RLRFLINWLV SLLQL++P+S G    NLVQIYGPKIKYERG
Sbjct: 781 VCRHIDHVNMLGLNKTTTRLRFLINWLVISLLQLQVPES-GGRHMNLVQIYGPKIKYERG 840

Query: 841 AAVAFNVRDKVRGLINPEIVQKLAEREGISLGIGFLSHIRIVD-NPRHHKGLNLDDTTLC 900
           AAVAFNVRDK +G ++PEIVQ+L +REG+SLGIG LSHIRIVD  PR+H+    +D+ L 
Sbjct: 841 AAVAFNVRDKSKGFVSPEIVQRLGDREGVSLGIGILSHIRIVDEKPRNHRARTKEDSALH 889

Query: 901 RPMENGKLGGKSGLMRVEVVTASLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEEG 938
              E     GK+G +R EVVTASL FLTNFEDVY+LW FVAKFLNP F +EG+LPTVEE 
Sbjct: 901 LQNE----AGKNGFIRFEVVTASLSFLTNFEDVYKLWVFVAKFLNPGFSREGSLPTVEEE 889

BLAST of Lsi01G010120 vs. TAIR 10
Match: AT5G66950.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1067.4 bits (2759), Expect = 6.6e-312
Identity = 595/944 (63.03%), Postives = 720/944 (76.27%), Query Frame = 0

Query: 1   MHLSLWKNLSHCAAALLMDKKGRRRDGSATGHDSKKNSSIL-RKLQENKLREALEEASEN 60
           MH+SLWK + HCAAAL++DKK      S +   S +N  +  RKL E+KLREALE+ASE+
Sbjct: 1   MHISLWKPIYHCAAALVLDKK-----SSGSSSSSSRNRDVTQRKLHESKLREALEQASED 60

Query: 61  GSLFKSQDIDESESLGDQDDGGGLGRSRSLARLHAQREFLRATALAAERTFESEDAIPDL 120
           G L KSQD++E +   DQ     LGRSRSLARL+AQREFLRAT+LAA+R FESE+ +P+L
Sbjct: 61  GLLVKSQDMEEEDESQDQI----LGRSRSLARLNAQREFLRATSLAAQRAFESEETLPEL 120

Query: 121 HESFSKFLTMYPNYQSSEKIDQLRSNEYSHLS-SKVCLDYCGFGLFSYVQTLHYWESSTF 180
            E+ + FLTMYP YQSSEK+D+LR++EY HLS  KVCLDYCGFGLFSY+QT+HYW++ TF
Sbjct: 121 EEALTIFLTMYPKYQSSEKVDELRNDEYFHLSLPKVCLDYCGFGLFSYLQTVHYWDTCTF 180

Query: 181 SLSEITANLSNHALYGGAEKGTVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADS 240
           SLSEI+ANLSNHA+YGGAEKG++EHDIK++IMDYLNIPE+EYGLVFTVSRGSAFKLLA+S
Sbjct: 181 SLSEISANLSNHAIYGGAEKGSIEHDIKIRIMDYLNIPENEYGLVFTVSRGSAFKLLAES 240

Query: 241 YPFHTNKKLLTMFDYESQSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRR 300
           YPFHTNKKLLTMFD+ESQSVSWMGQCA+EKGAKV SAWFKWP+L+LCS DL+K+I +K++
Sbjct: 241 YPFHTNKKLLTMFDHESQSVSWMGQCAKEKGAKVGSAWFKWPTLRLCSMDLKKEILSKKK 300

Query: 301 KKKDCATGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRP 360
           +KKD ATGLFVFPVQSRVTG+KYSYQWMALAQQNNWHVLLDAG+LGPKDMDSLGLSLFRP
Sbjct: 301 RKKDSATGLFVFPVQSRVTGSKYSYQWMALAQQNNWHVLLDAGALGPKDMDSLGLSLFRP 360

Query: 361 DFIVTSFYRVFGFDPTGFGCLLIKKSVMASLQNHSGSTGSGMVKITPEYPLYLSDSADGL 420
           DFI+TSFYRVFG+DPTGFGCLLIKKSV++ LQ+ SG T SG+VKITPEYPLYLSDS DGL
Sbjct: 361 DFIITSFYRVFGYDPTGFGCLLIKKSVISCLQSQSGKTSSGIVKITPEYPLYLSDSMDGL 420

Query: 421 DGFAGIEDDGVSENVDKASETRQKSQLPAFSGAFTSSQVREVFETEMD---SSERDGTST 480
           +G  GI+D+G++ N D  +     +QLPAFSGA+TS+QV++VFET+MD    S+RD TS 
Sbjct: 421 EGLTGIQDNGIAINGDNKA---LGTQLPAFSGAYTSAQVQDVFETDMDHEIGSDRDNTSA 480

Query: 481 IFEESESISVGEVMKSPVFSEDESSDNSLWIDLGHSPLGSDNAGFSKHEIASPLPPYWFA 540
           +FEE+ESISVGE++KSPVFSEDESSD+SLWIDLG SP  SDNAG    +  SPL      
Sbjct: 481 VFEEAESISVGELIKSPVFSEDESSDSSLWIDLGQSPADSDNAGHLNKQ-KSPL-----L 540

Query: 541 YRKN--RRQSPKPTSKIYSSPLYDDKEVNSRPGDERNMLSFDAAVMSVSQELDRYKEVGG 600
            RKN  RR SPKP SK            N+     R++LSFDAAV+SVS E+   +EV  
Sbjct: 541 VRKNHKRRSSPKPASK-----------ANNGSNGGRHVLSFDAAVLSVSHEVG--EEVIE 600

Query: 601 GKDSGMSPASQSGKKCIAHTDVLEVDKTSKTLSNGISSCDVKRSHLDNSTSLSQHHNLEN 660
            ++S M+    S +        L V +  +    G             S+ L+ H    N
Sbjct: 601 EENSEMNQIDTSRR--------LRVTEIEEEEEEG------------GSSKLTAH---AN 660

Query: 661 GSTSEICSEIKESAIRRETEGEFRLLGCR-GNRFAGGRFFGVDESEVQNKGRRVSFGIED 720
           GS+    S IK+SAIRRETEGEFRLLG R  +++ GGR   V+E E  +K RRVSF   D
Sbjct: 661 GSS----SGIKDSAIRRETEGEFRLLGRREKSQYNGGRLL-VNEDEHPSK-RRVSFRSVD 720

Query: 721 NGKEHQSCNLEPGETSVTSFDDDEGTSDGEYGDGEDWDRREPEIICRHIDHIDLLGLNKT 780
           +           GE SV S  D++   DG  G   D D+REPEI+CRHIDH+++LGLNKT
Sbjct: 721 H-----------GEASVISLGDEDEEEDGSNGVEWDDDQREPEIVCRHIDHVNMLGLNKT 780

Query: 781 TLRLRFLINWLVTSLLQLRLP--DSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGL 840
           T RLR+LINWLVTSLLQLRLP  DSDG  + NLVQIYGPKIKYERG++VAFN+RD   G+
Sbjct: 781 TSRLRYLINWLVTSLLQLRLPRSDSDGEHK-NLVQIYGPKIKYERGSSVAFNIRDLKSGM 840

Query: 841 INPEIVQKLAEREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLM 900
           ++PEIVQKLAEREGISLGIG+LSHI+I+DN         +D++  +P++  + G  +G +
Sbjct: 841 VHPEIVQKLAEREGISLGIGYLSHIKIIDN-------RSEDSSSWKPVD--REGRNNGFI 863

Query: 901 RVEVVTASLGFLTNFEDVYRLWAFVAKFLNPTFIKEGALPTVEE 935
           RVEVVTASLGFLTNFEDVYRLW FVAKFL+P F K+G LPTV E
Sbjct: 901 RVEVVTASLGFLTNFEDVYRLWNFVAKFLSPGFAKQGTLPTVIE 863

BLAST of Lsi01G010120 vs. TAIR 10
Match: AT5G51920.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 5.8e-82
Identity = 151/326 (46.32%), Postives = 217/326 (66.56%), Query Frame = 0

Query: 95  QREFLRAT--ALAAERTFESEDAIPDLHESFSKFLTMYPNYQSSEKIDQLRSNEYSH--L 154
           +R F + T   +  +  F   +++P   ESFS F+  YPNY  + KID+LRS+ Y H  L
Sbjct: 46  RRNFAQTTVSTIFPDTEFTDPNSLPSHQESFSDFIQAYPNYSDTYKIDRLRSDHYFHLGL 105

Query: 155 SSKVCLDYCGFGLFSYVQTLHY-----------WESSTFSLSEITANLSNHALYGGAEKG 214
           S   CLDY G GL+SY Q L+Y            ES  FS+S    NL    L  G ++ 
Sbjct: 106 SHYTCLDYIGIGLYSYSQLLNYDPSTYQISSSLSESPFFSVSPKIGNLKEKLLNDGGQET 165

Query: 215 TVEHDIKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYPFHTNKKLLTMFDYESQSVS 274
             E+ +K +IM +L I E +Y +VFT +R SAF+L+A+SYPF++ +KLLT++DYES++VS
Sbjct: 166 EFEYSMKRRIMGFLKISEEDYSMVFTANRTSAFRLVAESYPFNSKRKLLTVYDYESEAVS 225

Query: 275 WMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKKKDCATGLFVFPVQSRVTGA 334
            + + + ++GAKV +A F WP LKLCS+ LRK ++  +   K    G++VFP+ SRVTG+
Sbjct: 226 EINRVSEKRGAKVAAAEFSWPRLKLCSSKLRKLVTAGKNGSKTKKKGIYVFPLHSRVTGS 285

Query: 335 KYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIVTSFYRVFGFDPTGFGCL 394
           +Y Y WM++AQ+N WHV++DA  LGPKDMDS GLS++ PDF+V SFY+VFG +P+GFGCL
Sbjct: 286 RYPYLWMSVAQENGWHVMIDACGLGPKDMDSFGLSIYNPDFMVCSFYKVFGENPSGFGCL 345

Query: 395 LIKKSVMASLQNHSGSTGSGMVKITP 406
            +KKS ++ L++   STG GM+ + P
Sbjct: 346 FVKKSTISILES---STGPGMINLVP 368


HSP 2 Score: 119.0 bits (297), Expect = 2.0e-26
Identity = 76/180 (42.22%), Postives = 105/180 (58.33%), Query Frame = 0

Query: 745 DGEDWDRREPEIICRHIDHIDLLGLNKTTLRLRFLINWLVTSLLQLRLPDSDGSSRANLV 804
           D E+       +  + +DH+D LGL  T  R R LINWLV++L +L+      S+ + LV
Sbjct: 386 DSEETYSFSSSVEYKGLDHVDSLGLVATGNRSRCLINWLVSALYKLK-----HSTTSRLV 445

Query: 805 QIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQKLAEREGISLGIGFLSHIRIVDNPRHH 864
           +IYGPK+ + RG AVAFN+ +     I P IVQKLAE   ISLG  FL +I   ++   +
Sbjct: 446 KIYGPKVNFNRGPAVAFNLFNHKGEKIEPFIVQKLAECSNISLGKSFLKNILFQED---Y 505

Query: 865 KGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTASLGFLTNFEDVYRLWAFVAKFLNPTFI 924
           +G+        R  E  K        R+ V+TA+LGFL NFEDVY+LW FVA+FL+  F+
Sbjct: 506 EGVK------DRVFEK-KRNRDVDEPRISVLTAALGFLANFEDVYKLWIFVARFLDSEFV 550

BLAST of Lsi01G010120 vs. TAIR 10
Match: AT4G22980.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 9 growth stages; BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT5G51920.1); Has 520 Blast hits to 468 proteins in 130 species: Archae - 5; Bacteria - 23; Metazoa - 99; Fungi - 131; Plants - 231; Viruses - 0; Other Eukaryotes - 31 (source: NCBI BLink). )

HSP 1 Score: 227.3 bits (578), Expect = 5.3e-59
Identity = 129/315 (40.95%), Postives = 188/315 (59.68%), Query Frame = 0

Query: 86  SRSLARLHAQREFLRATA----LAAERTFESEDAIPDLHESFSKFLTMYPNYQSSEKIDQ 145
           S S++    + EF   T     L     F S++++P L  SF   +T +P+Y  + + D 
Sbjct: 24  SHSMSEKPEELEFSVTTTGTSFLTRNTKFTSQESLPRLRTSFYDLITAFPDYLQTNQADH 83

Query: 146 LRSNEYSHLSSKVCLDYCGFGLFSYVQTLHYWESSTFSLSEITANLSNHALYGGAEKGTV 205
           LRS EY +LSS   +      LFSY Q     ES +  L+     LS   +  G E  + 
Sbjct: 84  LRSTEYQNLSSSSHVFGQQQPLFSYSQFREISESES-DLNHSLLTLSCKQVSSGKELLSF 143

Query: 206 EHD------IKLKIMDYLNIPEHEYGLVFTVSRGSAFKLLADSYPFHTNKKLLTMFDYES 265
           E +      I+ +I  ++N+ E EY ++ T  R SAFK++A+ Y F TN  LLT+++YE 
Sbjct: 144 EEESRFQSRIRKRITSFMNLEESEYHMILTQDRSSAFKIVAELYSFKTNPNLLTVYNYED 203

Query: 266 QSVSWMGQCAREKGAKVYSAWFKWPSLKLCSTDLRKQISNKRRKKKDCATGLFVFPVQSR 325
           ++V  M + + +KG K  SA F WPS ++ S  L+++I+  +R+ K    GLFVFP+QS 
Sbjct: 204 EAVEEMIRISEKKGIKPQSAEFSWPSTEILSEKLKRRITRSKRRGK---RGLFVFPLQSL 263

Query: 326 VTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIVTSFYRVFG-FDPT 385
           VTGA YSY WM+LA+++ WHVLLD  +LG KDM++LGLSLF+PDF++ SF  V G  DP+
Sbjct: 264 VTGASYSYSWMSLARESEWHVLLDTSALGSKDMETLGLSLFQPDFLICSFTEVLGQDDPS 323

Query: 386 GFGCLLIKKSVMASL 390
           GFGCL +KKS   +L
Sbjct: 324 GFGCLFVKKSSSTAL 334


HSP 2 Score: 94.0 bits (232), Expect = 7.0e-19
Identity = 66/210 (31.43%), Postives = 110/210 (52.38%), Query Frame = 0

Query: 723 EPGETSVTSFDDDE----GTSDGEYGDGEDWDRREPEII-CRHIDHIDLLGLNKTTLRLR 782
           + G   +T  D ++     TS  E  + E   +++  +I  + +DH D LGL   + R +
Sbjct: 360 QAGYNEITPVDHEDHKAASTSSSEIVEIESSVKQDKAMIEFQGLDHADSLGLILISRRSK 419

Query: 783 FLINWLVTSLLQLRLPDSDGSSRANLVQIYGPKIKYERGAAVAFNVRDKVRGLINPEIVQ 842
            L  WL+ +L  L+ P     +   LV++YGPK K  RG +++FN+ D     ++P +V+
Sbjct: 420 SLTLWLLRALRTLQHPGYH-QTEMPLVKLYGPKTKPSRGPSISFNIFDWQGEKVDPLMVE 479

Query: 843 KLAEREGISLGIGFLSHIRIVDNPRHHKGLNLDDTTLCRPMENGKLGGKSGLMRVEVVTA 902
           +LAERE I L   +L   RI +  R  + ++L                     R+ VVT 
Sbjct: 480 RLAEREKIGLRCAYLHKFRIGNKRRSDEAVSL---------------------RLSVVTV 539

Query: 903 SL-GFLTNFEDVYRLWAFVAKFLNPTFIKE 927
            L GF+TNFEDV+++W FV++FL+  F+++
Sbjct: 540 RLGGFMTNFEDVFKVWEFVSRFLDADFVEK 547

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LGM79.8e-1825.57Molybdenum cofactor sulfurase OS=Solanum lycopersicum OX=4081 GN=FLACCA PE=2 SV=... [more]
Q96EN82.7e-1529.80Molybdenum cofactor sulfurase OS=Homo sapiens OX=9606 GN=MOCOS PE=1 SV=2[more]
B0Y6913.0e-1427.34Molybdenum cofactor sulfurase OS=Neosartorya fumigata (strain CEA10 / CBS 144.89... [more]
Q4WPE63.0e-1427.34Molybdenum cofactor sulfurase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af... [more]
Q7QFL76.6e-1426.69Molybdenum cofactor sulfurase OS=Anopheles gambiae OX=7165 GN=mal PE=3 SV=5[more]
Match NameE-valueIdentityDescription
A0A5D3CRF40.0e+0097.12Pyridoxal phosphate-dependent transferases superfamily protein OS=Cucumis melo v... [more]
A0A1S3B1850.0e+0097.12uncharacterized protein LOC103484742 OS=Cucumis melo OX=3656 GN=LOC103484742 PE=... [more]
A0A0A0KI550.0e+0097.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490000 PE=4 SV=1[more]
A0A6J1IJ460.0e+0093.30uncharacterized protein LOC111477906 OS=Cucurbita maxima OX=3661 GN=LOC111477906... [more]
A0A6J1GE670.0e+0093.30uncharacterized protein LOC111453345 OS=Cucurbita moschata OX=3662 GN=LOC1114533... [more]
Match NameE-valueIdentityDescription
XP_038881711.10.0e+0097.65uncharacterized protein LOC120073139 [Benincasa hispida][more]
XP_008440224.10.0e+0097.12PREDICTED: uncharacterized protein LOC103484742 [Cucumis melo] >TYK12919.1 Pyrid... [more]
XP_004141937.10.0e+0097.33uncharacterized protein LOC101221457 [Cucumis sativus] >KGN48484.1 hypothetical ... [more]
XP_023543927.10.0e+0093.52uncharacterized protein LOC111803649 [Cucurbita pepo subsp. pepo][more]
KAG6604205.10.0e+0093.41Molybdenum cofactor sulfurase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G23520.10.0e+0065.61Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G37100.10.0e+0065.49Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT5G66950.16.6e-31263.03Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT5G51920.15.8e-8246.32Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G22980.15.3e-5940.95FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 177..424
e-value: 2.7E-18
score: 68.0
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 754..924
e-value: 1.2E-5
score: 27.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..568
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 714..746
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..82
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 729..743
NoneNo IPR availablePANTHERPTHR14237:SF14PYRIDOXAL PHOSPHATE (PLP)-DEPENDENT TRANSFERASES SUPERFAMILY PROTEINcoord: 33..937
NoneNo IPR availablePANTHERPTHR14237MOLYBDOPTERIN COFACTOR SULFURASE MOSCcoord: 33..937
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 152..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G010120.1Lsi01G010120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016740 transferase activity
molecular_function GO:0003824 catalytic activity