Lcy13g010730 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g010730
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRhodanese domain-containing protein
LocationChr13: 11514702 .. 11519012 (+)
RNA-Seq ExpressionLcy13g010730
SyntenyLcy13g010730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAAGAAGGCGAACGACTCGTCGTCCACAGCCTCCATGCTCAGATATAGAGCTTCTCCGTTGCTAGCCCTGGGAATGGTATCGCCATTGCTTTCTTCTTCTTCTTCTTCCCTTTCTTCAAACCCTAATCTCATTTCATTCTCTCCCTCTTTCCCTATTCTGAAACCATCCCCTCCTCTACCTTCGCTTCTCTCCTCTTTCCTTCAGAAGCCCTCTCGGAGCATGACTCATTCCACATGCTTCGGGCCCTCCCCCGCCGTTCCCATTCCGACCGCATGTCAAGCGGCGGAGATTGAATCAGTAACGTCTCTCGTCGTTGTGTCCTTCTACAAGTTTGCTGATTTTCCTGACCATGCCGAATTTCGCTCGCCCTTGAAGAAGCTCTGCGAGGAACTGGTACTTGTTTTGTCTTGTCTTGTGTGTTTTTTGTAGAAGTTTGTTTGTTTTCCTTGTGTTTGTTTCTTGATTAGGAGGAAATGATGCTGAAGGATTTGGGGATTTGGGGGTTTTTTTTGAAGTGATTTCTGGTTTTATAGTGGAAGGGTTAATGTTGTGAAGTGATTGACTTGGAATAGGGCTGAATTTCTGGTTCTGTAAGCTAATGGAAGTTGGTGTTGTAATATAAGTTAACACCAAAGTTTAGGTTGTTGATTCCTTAAGAGCATTCCTTCCTGCTGGAAGGCTCCAACTTCTGAAGATAATCACTACTGATTTTAATACACTGATTGCTTCACGTAAGATTCGAAAGTTTCTTATTTTCACCTTAATATGTGCGTGTAGTCTGCGAAATGAAGTTTCAGGTTGAGTGATATGTGTAAGGAGTTCTGCATTTGGATTATTCAAAGGATCTTGCAAGTTTTCTTTTTAACATGATTGAGCAAAGCTCCTTCTAAGCACCCTATTCGTCCTATAAATTCCCCCTATATCTTTCATAGAAGGTTCTCCAAAGAATCACAAAGAAATTTGTCTGCCACAAAAGTTCTCCTCTCCTGCAAAGGGGATGCAAAAGAACCACCTCTATCTTAGCTAATATTATTCCGATGAGGAACCTGAATCATTCTGAAAGAGCTCCAAAAGCACTTGTAGCAAAAGAAACACCTCCATGAAATATCTTTGAGATCCTCTTGATTCCTATTTACACAACAAACACGAAGGCAGGCCAAGTACCTGAGAAGAAAAGCTGTACAGATAAACAGTCGTGTTAGTCCTCCCTAAAGAAACCAACCAAGAGTTTATTGGTAAGGGGGAGCTAACGAGCAAGAAAAGGCCCCTTCCTATATCAAGAGATAGGCTAAGGCGCCTTCACGAATATTCTAATAGTTGGCCCCTCTTTCATTTTTTGTTATTGAAAAGGGGCAACTTTCTCACTTGTTGCTTCAGAAAGATGGTAGGGGCGCGTTAGCACTTGACTAATAGTTTCTCGACGGCTCCTTCCCCTCTTTCACGAGGTAAAACAGCTTTGCTCCTTCCCTTCGTAAACTCGGTAAAGTGGCTTCACTCCTCTCCTTTCCTACGAATCTACAACTCTCCTTAAAAAACCTGACCTTCTTTAGGGTCTTAGCCTTTCAAAGGTTGGAGTAGCAATGGATAGGAAAAGGAGATGAGAAGGAAAGAGAAAAGCTCAAATAGCAGAAGTGAAAAGCTTGATGAAATAAGATTTGCACTAAAATACTCAGACCGACAGCGATCATGTAAACCCTAGACACAGACTTACCTTCAAAATTAAGGATATAAGGGAGGTTGAATCTTCCACCTCTTTATTGGGGAGAATCAGAAAAAAAGATAGATGAGGAGAATCCTTCAAAACAAGGAAAGTCTGAGGCTACAGATGCTCCCTTGACAAGGTCTAAGTTGTACAGACTGGTAATAGCAAACAAAGGGAGCACCGTGAATCCAGTGGACTGTGGTTGTCCCAAACTCTAGTTGATTTGTTTTTCATTGTTTCGGGAAGTAGATGAACAACTATTTCTTATCCGACAAAGATGAATAATCATTCTTATAGGATTTGATCTTCAGTTCTTAGTGATTAGTGAGTACATATAGTTATTAGCTTTAGTCTAAAAAAAATATATATATAGTTATTAGCTGGATATGTTTAATATATTCTTGTAAAAACTGAAAGATTTGGAAATAAAGAACCCTTATCATTTCATGAAGTTGAAAAATGACCTTCAGTTTCTTTTTCCCAGTACAAAGAATGGAGTGAATTTCTTAATATAGCTGCTATTAGAGAATGGGTATGGAAACAATTTCTGTTTTCTTTTCCAGTTTCTAGACTTCATCCGTTATTACACCCCTTTTTTCTGCCCTGTTTGGATTATGTTACTAAGCTAATCTTGCTCAGCGTGTTTCAGGAGGTATCATTCTTGCTCCTGAAGGAATTAATGGAAGCATATGTGGAACTCGAGAATCTGTTCAAAGGGTTCTTGGATTCATTGAAAGTGATGAACGACTAAAGGGACTAAGGCAGGTGGAAACACCTGTTAGTCCTGAGGAAGAAGCCATCCATCATGGACACACCAGCAGTTCCCCTCTTGCAGCCGGGGAAGATGCACCCTTCCGTTGGGATCATGTCAGGGTCAAATTAAAGAAAGAGGTACTCAAAATTTAATTTATTTTCATTGTTTTCTTGAGGATAGTGGGATGAAGGTTGAAATATAAGGCGCAGATCGGTTTTCTTATTTGTTGATTTTAATTTATTTATTGATGCTCTTGTCTCCATGCTACTTCTGTTTTTGCAGATTGTTACTCTTGGAATGCCTGTTATATCACCTGTTGAGAGGGTTGGAAAATATGTGAAACCTAGGGATTGGAATGTATTGATAAGTGACCCAGATATTGTGAGTGATTTTACCTGTCTTAGTTTTAATTCATATTGTTTAGTTGACATTACCATTAGCTTCTGTGGAATTTGCGTTGTATGTATTTGAATTTGAGTTTTCACATAAAAAATTAGGACATTCTCTTATTTTACTGCACTTATTTTGATTCTTAAATTTTTACTAAACTTGAGTGTGTTGCCACGTTAGGTTCTGCATTTGGTTAAATGAGCATATATAGCATATATAGTGTTGTGTCAGGTGGAACATACAGTGATTTTAGACTTTTTTTCCTTGAAAGGAATAAGATATGAACATGCTACATATTTAACGCTAATGTAGGTGGTCATTGATGTGCGAAATCATTACGAAACAAGGATCGGGAAGTTCAAAAGAGCAGTTGATCCTTGTACTACAGCATTTCGAGACTTCCCGGTTTGGGTAGAGGATGAATTCCAACTTCCTAAATCAGATAACAAGCATTCAAATCAAGGTGTCGAGGAAGAAGCAGTAAACCCAGAACATAACTCGCTTCCGCGAGTTGCCATGTACTGCACTGGAGGAATCCGTTGTGAGAAGGCTTCTAGTTATCTCCTCAGCAAAGGCTTTAAAGAGGTATATATAAACATGGTTTGCTTTCTGATGAACTTTGTACGTGACACATGTGACTGAAAGGTTTAACCTGAGGCATTATTTCATTTGAATATTTTCTAAGGTTTATCATTTGGAAGGTGGAATTCTGAAATATCTGGAAGAAGTTCCAAAGACAGAGAGCCTGTGGGAAGGAGAATGCTTTGTATTTGACAAGCGTGTCTCGGTTGAGCATGGTCTGGAGCAGGGCACATTCAAGCTCTGTTATGGGTGCAAGCAACCAGTGAGTGATGCTGACATGGAATCCCCCGAGTGGGAGTATGGAGTTTCATGTCCACACTGTTACTCAACGAAATCTGAAGAAGAGAAGGAGAGGGCAAGAGCTCGGCAAAGGCAATTTGAGACTTGGGGCATCATTGGTGGTCCGGACAAAGGCCGCCGACCAAAGGAAGGTGATAACAATCTTAATCTTCCGAATTCAGTTTGAAGTTTTCGTTTTTGTATTAGGGGATTATACAAAGGGGGCCGATCAAAATACCCTTGATTGTGACAAAACTAAGTTGGAAAAGCTTGGAACTTCCTTGTCTCTCTGTTTTCACGACATGTCAGCAACAAAGGCAAACTGGAAATCGTCCTTAATGTTGTAGAGGAGAAGAAAAAATTGATGACGTTGTAGTGTCTATGATGACTAGCAAGAGCATGCGCGGAGTTGTGGATTTGTGAGCAATTTTTTTTTAAAAAGAAATAACATATGGATGGAGGATTTGAAACTCTGATTTTTTATCGAGGATATAAGTTATATGTCAGTTGAGTTATACTCTTTCATAAAAGTGTAGGGACTAAATTGTTATTTTTATGAAAGTTTAGGGATTAAATTGTTATAAATTTTGAAGTACGA

mRNA sequence

ATGAGAAAGAAGGCGAACGACTCGTCGTCCACAGCCTCCATGCTCAGATATAGAGCTTCTCCGTTGCTAGCCCTGGGAATGGTATCGCCATTGCTTTCTTCTTCTTCTTCTTCCCTTTCTTCAAACCCTAATCTCATTTCATTCTCTCCCTCTTTCCCTATTCTGAAACCATCCCCTCCTCTACCTTCGCTTCTCTCCTCTTTCCTTCAGAAGCCCTCTCGGAGCATGACTCATTCCACATGCTTCGGGCCCTCCCCCGCCGTTCCCATTCCGACCGCATGTCAAGCGGCGGAGATTGAATCAGTAACGTCTCTCGTCGTTGTGTCCTTCTACAAGTTTGCTGATTTTCCTGACCATGCCGAATTTCGCTCGCCCTTGAAGAAGCTCTGCGAGGAACTGCGTGTTTCAGGAGGTATCATTCTTGCTCCTGAAGGAATTAATGGAAGCATATGTGGAACTCGAGAATCTGTTCAAAGGGTTCTTGGATTCATTGAAAGTGATGAACGACTAAAGGGACTAAGGCAGGTGGAAACACCTGTTAGTCCTGAGGAAGAAGCCATCCATCATGGACACACCAGCAGTTCCCCTCTTGCAGCCGGGGAAGATGCACCCTTCCGTTGGGATCATGTCAGGGTCAAATTAAAGAAAGAGATTGTTACTCTTGGAATGCCTGTTATATCACCTGTTGAGAGGGTTGGAAAATATGTGAAACCTAGGGATTGGAATGTATTGATAAGTGACCCAGATATTGTGGTCATTGATGTGCGAAATCATTACGAAACAAGGATCGGGAAGTTCAAAAGAGCAGTTGATCCTTGTACTACAGCATTTCGAGACTTCCCGGTTTGGGTAGAGGATGAATTCCAACTTCCTAAATCAGATAACAAGCATTCAAATCAAGGTGTCGAGGAAGAAGCAGTAAACCCAGAACATAACTCGCTTCCGCGAGTTGCCATGTACTGCACTGGAGGAATCCGTTGTGAGAAGGCTTCTAGTTATCTCCTCAGCAAAGGCTTTAAAGAGGTTTATCATTTGGAAGGTGGAATTCTGAAATATCTGGAAGAAGTTCCAAAGACAGAGAGCCTGTGGGAAGGAGAATGCTTTGTATTTGACAAGCGTGTCTCGGTTGAGCATGGTCTGGAGCAGGGCACATTCAAGCTCTGTTATGGGTGCAAGCAACCAGTGAGTGATGCTGACATGGAATCCCCCGAGTGGGAGTATGGAGTTTCATGTCCACACTGTTACTCAACGAAATCTGAAGAAGAGAAGGAGAGGGCAAGAGCTCGGCAAAGGCAATTTGAGACTTGGGGCATCATTGGTGGTCCGGACAAAGGCCGCCGACCAAAGGAAGGTGATAACAATCTTAATCTTCCGAATTCAGTTTGAAGTTTTCGTTTTTGTATTAGGGGATTATACAAAGGGGGCCGATCAAAATACCCTTGATTGTGACAAAACTAAGTTGGAAAAGCTTGGAACTTCCTTGTCTCTCTGTTTTCACGACATGTCAGCAACAAAGGCAAACTGGAAATCGTCCTTAATGTTGTAGAGGAGAAGAAAAAATTGATGACGTTGTAGTGTCTATGATGACTAGCAAGAGCATGCGCGGAGTTGTGGATTTGTGAGCAATTTTTTTTTAAAAAGAAATAACATATGGATGGAGGATTTGAAACTCTGATTTTTTATCGAGGATATAAGTTATATGTCAGTTGAGTTATACTCTTTCATAAAAGTGTAGGGACTAAATTGTTATTTTTATGAAAGTTTAGGGATTAAATTGTTATAAATTTTGAAGTACGA

Coding sequence (CDS)

ATGAGAAAGAAGGCGAACGACTCGTCGTCCACAGCCTCCATGCTCAGATATAGAGCTTCTCCGTTGCTAGCCCTGGGAATGGTATCGCCATTGCTTTCTTCTTCTTCTTCTTCCCTTTCTTCAAACCCTAATCTCATTTCATTCTCTCCCTCTTTCCCTATTCTGAAACCATCCCCTCCTCTACCTTCGCTTCTCTCCTCTTTCCTTCAGAAGCCCTCTCGGAGCATGACTCATTCCACATGCTTCGGGCCCTCCCCCGCCGTTCCCATTCCGACCGCATGTCAAGCGGCGGAGATTGAATCAGTAACGTCTCTCGTCGTTGTGTCCTTCTACAAGTTTGCTGATTTTCCTGACCATGCCGAATTTCGCTCGCCCTTGAAGAAGCTCTGCGAGGAACTGCGTGTTTCAGGAGGTATCATTCTTGCTCCTGAAGGAATTAATGGAAGCATATGTGGAACTCGAGAATCTGTTCAAAGGGTTCTTGGATTCATTGAAAGTGATGAACGACTAAAGGGACTAAGGCAGGTGGAAACACCTGTTAGTCCTGAGGAAGAAGCCATCCATCATGGACACACCAGCAGTTCCCCTCTTGCAGCCGGGGAAGATGCACCCTTCCGTTGGGATCATGTCAGGGTCAAATTAAAGAAAGAGATTGTTACTCTTGGAATGCCTGTTATATCACCTGTTGAGAGGGTTGGAAAATATGTGAAACCTAGGGATTGGAATGTATTGATAAGTGACCCAGATATTGTGGTCATTGATGTGCGAAATCATTACGAAACAAGGATCGGGAAGTTCAAAAGAGCAGTTGATCCTTGTACTACAGCATTTCGAGACTTCCCGGTTTGGGTAGAGGATGAATTCCAACTTCCTAAATCAGATAACAAGCATTCAAATCAAGGTGTCGAGGAAGAAGCAGTAAACCCAGAACATAACTCGCTTCCGCGAGTTGCCATGTACTGCACTGGAGGAATCCGTTGTGAGAAGGCTTCTAGTTATCTCCTCAGCAAAGGCTTTAAAGAGGTTTATCATTTGGAAGGTGGAATTCTGAAATATCTGGAAGAAGTTCCAAAGACAGAGAGCCTGTGGGAAGGAGAATGCTTTGTATTTGACAAGCGTGTCTCGGTTGAGCATGGTCTGGAGCAGGGCACATTCAAGCTCTGTTATGGGTGCAAGCAACCAGTGAGTGATGCTGACATGGAATCCCCCGAGTGGGAGTATGGAGTTTCATGTCCACACTGTTACTCAACGAAATCTGAAGAAGAGAAGGAGAGGGCAAGAGCTCGGCAAAGGCAATTTGAGACTTGGGGCATCATTGGTGGTCCGGACAAAGGCCGCCGACCAAAGGAAGGTGATAACAATCTTAATCTTCCGAATTCAGTTTGA

Protein sequence

MRKKANDSSSTASMLRYRASPLLALGMVSPLLSSSSSSLSSNPNLISFSPSFPILKPSPPLPSLLSSFLQKPSRSMTHSTCFGPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV
Homology
BLAST of Lcy13g010730 vs. ExPASy Swiss-Prot
Match: Q1JPN0 (Rhodanese-like domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=STR7 PE=2 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 4.2e-163
Identity = 297/448 (66.29%), Postives = 334/448 (74.55%), Query Frame = 0

Query: 48  FSPSFPILK--PSPPLPSLLSSF-----------------LQKPSRSMTHSTCFGPSPAV 107
           F PS  +++   SPP P   S F                 LQ P +S  H     PS ++
Sbjct: 9   FPPSLAVVRMLSSPPPPHSHSPFSGGGVNSNSVGGNSKPELQFP-QSQPHKLSSSPSSSL 68

Query: 108 PIPTACQAA----------------EIESVT----SLVVVSFYKFADFPDHAEFRSPLKK 167
               AC  A                  ES+     SLVVVSFYKFADFP+HA+FR PLK 
Sbjct: 69  KSTVACSNAGAIRRSMATVSQAFSERTESIDSDLGSLVVVSFYKFADFPEHADFRKPLKD 128

Query: 168 LCEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIH 227
           LCE+LRVSGGIILAPEGINGSICG RESV+ VL FI+ D RL GLRQVETPVSPE+EAIH
Sbjct: 129 LCEKLRVSGGIILAPEGINGSICGIRESVEEVLAFIQRDVRLNGLRQVETPVSPEQEAIH 188

Query: 228 HGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDP 287
           HGH+SSSPLAAGEDAPFRWDHVRVKLKKEIVTLG+P +SP+ERVG YV P +WN LISDP
Sbjct: 189 HGHSSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGIPSVSPIERVGTYVSPEEWNELISDP 248

Query: 288 DIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVN 347
           + VVIDVRN YETRIGKFK AVDPCTTAFR+FP WVE++F L +  N+   +  +E+   
Sbjct: 249 ETVVIDVRNTYETRIGKFKGAVDPCTTAFRNFPSWVENQFALKQEGNETQAKVEKEDFSE 308

Query: 348 PEH--------NSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTE 407
             H         +LPR+AMYCTGGIRCEKASS LLS+GF+EVYHL+GGILKYLEEVPKTE
Sbjct: 309 ITHKEDKAEKPKTLPRIAMYCTGGIRCEKASSLLLSQGFEEVYHLKGGILKYLEEVPKTE 368

Query: 408 SLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSE 449
           SLWEGECFVFDKRVSVEHGL QGT KLCYGCKQP+SD DME+PE+EYGVSCP+CYS KSE
Sbjct: 369 SLWEGECFVFDKRVSVEHGLAQGTHKLCYGCKQPISDEDMEAPEYEYGVSCPYCYSKKSE 428

BLAST of Lcy13g010730 vs. ExPASy Swiss-Prot
Match: Q1GYV1 (tRNA uridine(34) hydroxylase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) OX=265072 GN=trhO PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.9e-84
Identity = 155/330 (46.97%), Postives = 208/330 (63.03%), Query Frame = 0

Query: 105 LVVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQRVLGFI 164
           +VV + YKF   PD+ E +  L  LC    + G ++LA EGING++ G+R ++  +L ++
Sbjct: 4   IVVAALYKFVSLPDYQELQPGLLALCNAQGIKGTLLLAEEGINGTVAGSRAAIDALLTYL 63

Query: 165 ESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMP 224
             D R+  +   E                    +  ++ PF    ++V+LKKEIVTLG+P
Sbjct: 64  RQDSRMVDIDHKE--------------------SFADEMPFY--RMKVRLKKEIVTLGVP 123

Query: 225 VISPVERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWV 284
            ISP ++VG YVKP DWN LISDPD+VVID RN+YE  IG FK A+DP TT FR FP +V
Sbjct: 124 GISPNKKVGTYVKPEDWNTLISDPDVVVIDTRNNYEYEIGTFKGAIDPATTTFRQFPEFV 183

Query: 285 EDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYH 344
                               + ++P  N   +VAM+CTGGIRCEKASSY+L +GFKEVYH
Sbjct: 184 -------------------RKNLDPAKNK--KVAMFCTGGIRCEKASSYMLEQGFKEVYH 243

Query: 345 LEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPE 404
           L+GGILKYLE +P+ ESLWEGECFVFD+RV+V+HGL+ G +  C+ C+ P+S ADM S +
Sbjct: 244 LQGGILKYLETIPEEESLWEGECFVFDQRVAVKHGLQVGDYDQCFACRHPISAADMASSK 290

Query: 405 WEYGVSCPHCYSTKSEEEKERARARQRQFE 435
           +  G+SCP CY   S E++ R   RQRQ E
Sbjct: 304 YVKGISCPRCYDRMSAEKRARIAERQRQIE 290

BLAST of Lcy13g010730 vs. ExPASy Swiss-Prot
Match: Q92LX6 (tRNA uridine(34) hydroxylase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=trhO PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 6.6e-84
Identity = 159/333 (47.75%), Postives = 212/333 (63.66%), Query Frame = 0

Query: 100 ESVTSLVVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQR 159
           E+    +V + Y F  FP  A+FR PL+ +C+   V G ++LA EGING+I GT E +  
Sbjct: 12  ETPGQFLVAALYHFVSFPRFADFREPLQAICDANGVKGTLLLAHEGINGTIAGTEEGIAA 71

Query: 160 VLGFIESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIV 219
           VL  + +     GL             IH    +S+        PF    ++V+LKKEIV
Sbjct: 72  VLACVRAQPEFAGL-------------IHKESRASA-------MPFL--RMKVRLKKEIV 131

Query: 220 TLGMPVISPVERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRIGKFKRAVDPCTTAFRD 279
           T+G+  I P + VG YV+P+DWN LISDP+ +VID RN YET IG F+ AVDP T  FR+
Sbjct: 132 TMGVENIDPKKVVGTYVEPKDWNALISDPETLVIDTRNDYETAIGLFQGAVDPKTKTFRE 191

Query: 280 FPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCEKASSYLLSKGF 339
           FP WV             +N G+        HN  P++AMYCTGGIRCEKA++++  +GF
Sbjct: 192 FPEWV------------RNNSGL--------HNK-PKIAMYCTGGIRCEKATAFMKEQGF 251

Query: 340 KEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDAD 399
           +EVYHL+GGILKYLEE+P  ESLWEG CFVFD+RVSV HGL++G   LC+ C+QP++  D
Sbjct: 252 EEVYHLKGGILKYLEEIPPEESLWEGACFVFDERVSVTHGLQEGEHTLCHACRQPLTPED 301

Query: 400 MESPEWEYGVSCPHCYSTKSEEEKERARARQRQ 433
           ++SP  E GVSC HC+ T++EE++ER R RQRQ
Sbjct: 312 LQSPLHEEGVSCIHCHDTRTEEDRERYRQRQRQ 301

BLAST of Lcy13g010730 vs. ExPASy Swiss-Prot
Match: Q8YZS3 (tRNA uridine(34) hydroxylase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=trhO PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 9.6e-83
Identity = 152/329 (46.20%), Postives = 211/329 (64.13%), Query Frame = 0

Query: 106 VVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQRVLGFIE 165
           +V +FYKF   PD AE + PL   C    + G I+LA EGING+I G+R S+  VL ++ 
Sbjct: 8   IVAAFYKFVSLPDFAEKQEPLLAYCLAQNIKGTILLAKEGINGTIAGSRLSIDNVLSYLR 67

Query: 166 SDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPV 225
           SD RL+ L              H   T+ +P       PF  + ++V+LKKEIVTLG+P 
Sbjct: 68  SDLRLQDLE-------------HKESTADTP-------PF--ERMKVRLKKEIVTLGLPE 127

Query: 226 ISPVERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVE 285
           + P E+VG YV P +WN LISDP+++VID RN YE  IG F+ A +P T +FRDFP +V 
Sbjct: 128 VDPNEQVGAYVTPEEWNELISDPEVIVIDTRNDYEVHIGTFQGAQNPQTNSFRDFPEYVR 187

Query: 286 DEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHL 345
                                 N + N   +VAM+CTGGIRCEKASS++LS+GF EVYHL
Sbjct: 188 Q---------------------NLDPNQHKKVAMFCTGGIRCEKASSFMLSQGFAEVYHL 247

Query: 346 EGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEW 405
           +GGILKYLE++P  ESLW+GECFVFD+R++V HGLE GT +LC+ C  P+++ D  S ++
Sbjct: 248 KGGILKYLEQIPPEESLWQGECFVFDERIAVVHGLEPGTHELCFCCGHPLAEEDKASLQY 293

Query: 406 EYGVSCPHCYSTKSEEEKERARARQRQFE 435
           E G+SCPHC+ + +E+++ R + + RQ++
Sbjct: 308 EEGISCPHCFDSLTEDKRMRQQEKWRQYQ 293

BLAST of Lcy13g010730 vs. ExPASy Swiss-Prot
Match: A6UD82 (tRNA uridine(34) hydroxylase OS=Sinorhizobium medicae (strain WSM419) OX=366394 GN=trhO PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.6e-82
Identity = 157/333 (47.15%), Postives = 213/333 (63.96%), Query Frame = 0

Query: 100 ESVTSLVVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQR 159
           E+    +V + Y F  FP  A+FR PL+ +C+   V G ++LA EGING+I G+ E +  
Sbjct: 12  ETPGQFLVAALYHFISFPRFADFREPLQAICDANGVKGTLLLAHEGINGTIAGSEEGIAT 71

Query: 160 VLGFIESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIV 219
           VL F+ +               PE   + H  + +S +      PF    ++V+LKKEIV
Sbjct: 72  VLAFLRA--------------QPEFADLVHKESRASAM------PFL--RMKVRLKKEIV 131

Query: 220 TLGMPVISPVERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRIGKFKRAVDPCTTAFRD 279
           T+G+  I P + VG YV+P+DWN LISDP+ +VID RN YET IG F+ AVDP T  FR+
Sbjct: 132 TMGVENIDPKKVVGTYVEPKDWNALISDPETLVIDTRNDYETAIGVFQGAVDPKTKTFRE 191

Query: 280 FPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCEKASSYLLSKGF 339
           FP WV             +N G+        HN  P++AMYCTGGIRCEKA++++  +GF
Sbjct: 192 FPEWV------------RNNSGL--------HNK-PKIAMYCTGGIRCEKATAFMKEQGF 251

Query: 340 KEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDAD 399
           +EVYHL+GGILKYLEE+P  ESLWEG CFVFD+RVSV HGL++G   LC+ C+QP++  D
Sbjct: 252 EEVYHLKGGILKYLEEIPPEESLWEGACFVFDERVSVTHGLQEGEHTLCHACRQPLTTED 301

Query: 400 MESPEWEYGVSCPHCYSTKSEEEKERARARQRQ 433
           + SP  E GVSC +C+ T++EE++ER R RQRQ
Sbjct: 312 LRSPLHEEGVSCVNCHDTRTEEDRERYRQRQRQ 301

BLAST of Lcy13g010730 vs. ExPASy TrEMBL
Match: A0A6J1FRM3 (rhodanese-like domain-containing protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111447816 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 404/452 (89.38%), Postives = 423/452 (93.58%), Query Frame = 0

Query: 14  MLRYRASPLLALGMVSPLL---SSSSSSLSSNPNLISFSPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA PLLALGMVSPLL   SSSSSS SSNPNLI+F+PSF +LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPLLALGMVSPLLTSSSSSSSSFSSNPNLITFNPSFLVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKL 133
           KP+RSMTHS CF GPSPAVPIPTAC+A E++SV SLVVVSFYKFADFPDHAEFRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH 193
           C+ELRVSGGIILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH
Sbjct: 121 CQELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH 180

Query: 194 GHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPD 253
           GH+S SPLAAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVER G+YVKPRDWN LISDPD
Sbjct: 181 GHSSGSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERAGQYVKPRDWNALISDPD 240

Query: 254 IVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNP 313
            VVIDVRNHYETRIGKFKRAVDPCT AFRDFP WVED+FQLPKSD +HSN+ VE + VNP
Sbjct: 241 TVVIDVRNHYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNP 300

Query: 314 EHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 373
           E NS PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV
Sbjct: 301 EQNSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 360

Query: 374 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARAR 433
           FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARAR
Sbjct: 361 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARAR 420

Query: 434 QRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           QRQFETWGIIGGPDKGRRPK+ D N  LPNS+
Sbjct: 421 QRQFETWGIIGGPDKGRRPKQSDKNATLPNSI 451

BLAST of Lcy13g010730 vs. ExPASy TrEMBL
Match: A0A6J1JDN9 (rhodanese-like domain-containing protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111485926 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 6.6e-228
Identity = 393/438 (89.73%), Postives = 413/438 (94.29%), Query Frame = 0

Query: 27  MVSPLLSSSS--SSLSSNPNLISFSPSFPILKPSPPLPSLLSSFLQKPSRSMTHSTCF-G 86
           MVSPLLSSSS  SS SSNPNLI+F+PSFPILKPSP LPSLLSSF QKPSRSMTHS CF G
Sbjct: 1   MVSPLLSSSSSYSSFSSNPNLITFNPSFPILKPSPTLPSLLSSFHQKPSRSMTHSKCFSG 60

Query: 87  PSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAP 146
           PSPAVPIP+AC+  E++S+ SLVVVSFYKFADFPDHAEFRSPLKKLC+ELRVSGGIILAP
Sbjct: 61  PSPAVPIPSACEVQEVDSM-SLVVVSFYKFADFPDHAEFRSPLKKLCQELRVSGGIILAP 120

Query: 147 EGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDA 206
           EGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGH+SSSPLAAGEDA
Sbjct: 121 EGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSSSPLAAGEDA 180

Query: 207 PFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRI 266
           PFRWDHVRVKLKKEIVTLGMP +SPVERVG+YVKPRDWN LISDPD VVIDVRNHYETRI
Sbjct: 181 PFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNTLISDPDTVVIDVRNHYETRI 240

Query: 267 GKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTG 326
           GKFKRAVDPCT AFRDFP WVED+FQLPKSD +HSN+ VE + VNPE NS PRVAMYCTG
Sbjct: 241 GKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEENSPPRVAMYCTG 300

Query: 327 GIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQG 386
           GIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQG
Sbjct: 301 GIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQG 360

Query: 387 TFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPD 446
           TFKLCYGCKQPVSDAD+ESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPD
Sbjct: 361 TFKLCYGCKQPVSDADIESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPD 420

Query: 447 KGRRPKEGDNNLNLPNSV 462
           KGRRPK+ D N  LPNS+
Sbjct: 421 KGRRPKQSDKNATLPNSI 437

BLAST of Lcy13g010730 vs. ExPASy TrEMBL
Match: A0A6J1CYE5 (rhodanese-like domain-containing protein 7 OS=Momordica charantia OX=3673 GN=LOC111015925 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.8e-224
Identity = 402/472 (85.17%), Postives = 424/472 (89.83%), Query Frame = 0

Query: 3   KKANDSSST-ASMLRYRASPLLALGMVSPL--------LSSSSSSLSSNPNLISFSPSFP 62
           K+ NDSSST ASM+RYRA   LA+   SPL         SSSSSS SSNPN I+FS SFP
Sbjct: 35  KEPNDSSSTRASMIRYRA---LAMVSPSPLPSSSSSSSSSSSSSSFSSNPNFITFSSSFP 94

Query: 63  ILKPSPPLPSLLSSF---LQKPSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVS 122
           +LKPS PLPSLLSSF   L+K +RSMT+S CF G + AVPIP+AC A E +SV SLVVVS
Sbjct: 95  VLKPSLPLPSLLSSFLSALRKHTRSMTYSRCFSGSTTAVPIPSACHAEETDSV-SLVVVS 154

Query: 123 FYKFADFPDHAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDER 182
           FYKFADFPDHA+FR PLKKLCEELRVSGGIILAPEGINGSICG+RESVQRVLGFIESDER
Sbjct: 155 FYKFADFPDHAQFRQPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDER 214

Query: 183 LKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPV 242
           LKGLRQVETPVSPEEEAIHHGH SSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPV
Sbjct: 215 LKGLRQVETPVSPEEEAIHHGHISSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPV 274

Query: 243 ERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQ 302
           ERVGKYVKPRDWN LISDPD VVIDVRNHYETRIGKFK AVDPCTTAFRDFP WVED+FQ
Sbjct: 275 ERVGKYVKPRDWNKLISDPDTVVIDVRNHYETRIGKFKSAVDPCTTAFRDFPSWVEDKFQ 334

Query: 303 LPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGI 362
           + KSD KHSNQGVEE+AVNPE N+ PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGI
Sbjct: 335 VSKSDRKHSNQGVEEDAVNPEQNAPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGI 394

Query: 363 LKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGV 422
           LKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGV
Sbjct: 395 LKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGV 454

Query: 423 SCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           SCP+CYSTKSEEEKERARARQRQFETWGIIGGPDKGR P   +N++NL NS+
Sbjct: 455 SCPYCYSTKSEEEKERARARQRQFETWGIIGGPDKGRSPNPSNNSVNLSNSI 502

BLAST of Lcy13g010730 vs. ExPASy TrEMBL
Match: A0A0A0KM63 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G223080 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.3e-217
Identity = 385/457 (84.25%), Postives = 407/457 (89.06%), Query Frame = 0

Query: 14  MLRYRASPLLALGMVSPLLSSSS------SSLSSNPNLIS--FSPSFPILKPSPPLPSLL 73
           ++RYRA PL  LGMVSP  SSSS      SS SSN NLIS  FSPSFPI +P    PSLL
Sbjct: 2   LIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPFLSFPSLL 61

Query: 74  SSFLQKPSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRS 133
           S+FL KP RSMTHS CF GPSP  PI ++ + +E     SLVVVSFYKFADFPDHAEFR 
Sbjct: 62  SAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRL 121

Query: 134 PLKKLCEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEE 193
           PLKKLCEELRVSGGIILAPEGINGSICG+RESVQRVLGFI+SDERLKGLRQVETPVSPEE
Sbjct: 122 PLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEE 181

Query: 194 EAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVL 253
           EAIH+GHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVG+YVKPRDWN L
Sbjct: 182 EAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNAL 241

Query: 254 ISDPDIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEE 313
           ISDPD VVIDVRNHYETRIGKFK AVDPCTTAFRDFP WVED+ QLPK D KHSNQG  E
Sbjct: 242 ISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQG-GE 301

Query: 314 EAVNPEHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWE 373
           E VNP+ +S PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPK+ESLWE
Sbjct: 302 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 361

Query: 374 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKE 433
           GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCP+C+STKSEEEKE
Sbjct: 362 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 421

Query: 434 RARARQRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           RARARQRQFETWGIIGGPDKGRRP + D+N++ PNSV
Sbjct: 422 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 457

BLAST of Lcy13g010730 vs. ExPASy TrEMBL
Match: A0A1S4E1C5 (LOW QUALITY PROTEIN: rhodanese-like domain-containing protein 7 OS=Cucumis melo OX=3656 GN=LOC103496761 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 8.6e-212
Identity = 379/451 (84.04%), Postives = 402/451 (89.14%), Query Frame = 0

Query: 14  MLRYRASPLLALGMVSPLLSSSSSSLSSNPNLIS--FSPSFPILKPSPPLPSLLSSFLQK 73
           ++RYRA PLLALGMVSPL SSSSSS SSN NLIS  FSPSFP+ KPS   PSLLSSF+ K
Sbjct: 2   LIRYRAPPLLALGMVSPLPSSSSSSFSSNTNLISFNFSPSFPVPKPSSSFPSLLSSFVHK 61

Query: 74  PSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKLC 133
           PSRSMTHS CF GPSP  PI ++C+ +E     SLVVVSFYKFA FPDHAEFRSPLKKLC
Sbjct: 62  PSRSMTHSICFSGPSPTAPIHSSCEESEDMDSISLVVVSFYKFAHFPDHAEFRSPLKKLC 121

Query: 134 EELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHG 193
           EELRV GGIILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVET VSPEEEAIH+G
Sbjct: 122 EELRVLGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETSVSPEEEAIHNG 181

Query: 194 HTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPDI 253
           HTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVG+YVKPRD N LISDPD 
Sbjct: 182 HTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDXNSLISDPDT 241

Query: 254 VVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNPE 313
           VV DV+NHYETRI KFK AVDP TTAFRDFP WVED+FQLPK D KHSNQG +EE VNPE
Sbjct: 242 VVTDVQNHYETRIEKFKTAVDPSTTAFRDFPAWVEDKFQLPKLDCKHSNQG-DEETVNPE 301

Query: 314 HNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVF 373
            +S  RVAMYCTGGIRCEKASSYLL KGFKEVYHLEGGILKYLEEVP++ESL EGECFVF
Sbjct: 302 ESSPLRVAMYCTGGIRCEKASSYLLRKGFKEVYHLEGGILKYLEEVPRSESLREGECFVF 361

Query: 374 DKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQ 433
           DKR SVEHGLEQGTFKLCYG KQPVSDADMESP+WEYGVSCP+C+S KS+EEKERARARQ
Sbjct: 362 DKRDSVEHGLEQGTFKLCYGYKQPVSDADMESPQWEYGVSCPYCFSMKSDEEKERARARQ 421

Query: 434 RQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           RQFETWGIIGGPDKGRRP + DNN+N  NSV
Sbjct: 422 RQFETWGIIGGPDKGRRPTQNDNNVNHLNSV 451

BLAST of Lcy13g010730 vs. NCBI nr
Match: KAG7031505.1 (Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 823.2 bits (2125), Expect = 1.1e-234
Identity = 406/452 (89.82%), Postives = 425/452 (94.03%), Query Frame = 0

Query: 14  MLRYRASPLLALGMVSPLL---SSSSSSLSSNPNLISFSPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA PLLALGMVSPLL   SSSSSS SSNPNLI+F+PSFP+LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPLLALGMVSPLLSSSSSSSSSFSSNPNLITFNPSFPVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKL 133
           KP+RSMTHS CF GPSPAVPIPTAC+A E++SV SLVVVSFYKFADFPDHAEFRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH 193
           C+ELRVSGGIILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH
Sbjct: 121 CQELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH 180

Query: 194 GHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPD 253
           GH+S SPLAAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVERVG+YVKPRDWN LISDPD
Sbjct: 181 GHSSGSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNALISDPD 240

Query: 254 IVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNP 313
            VVIDVRNHYETRIGKFKRAVDPCT AFRDFP WVED+FQLPKSD +HSN+ VE + VNP
Sbjct: 241 TVVIDVRNHYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNP 300

Query: 314 EHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 373
           E NS PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV
Sbjct: 301 EENSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 360

Query: 374 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARAR 433
           FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARAR
Sbjct: 361 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARAR 420

Query: 434 QRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           QRQFETWGIIGGPDKGRRPK+ D N  LPNS+
Sbjct: 421 QRQFETWGIIGGPDKGRRPKQSDKNATLPNSI 451

BLAST of Lcy13g010730 vs. NCBI nr
Match: XP_022942932.1 (rhodanese-like domain-containing protein 7 [Cucurbita moschata])

HSP 1 Score: 817.8 bits (2111), Expect = 4.8e-233
Identity = 404/452 (89.38%), Postives = 423/452 (93.58%), Query Frame = 0

Query: 14  MLRYRASPLLALGMVSPLL---SSSSSSLSSNPNLISFSPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA PLLALGMVSPLL   SSSSSS SSNPNLI+F+PSF +LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPLLALGMVSPLLTSSSSSSSSFSSNPNLITFNPSFLVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKL 133
           KP+RSMTHS CF GPSPAVPIPTAC+A E++SV SLVVVSFYKFADFPDHAEFRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH 193
           C+ELRVSGGIILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH
Sbjct: 121 CQELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH 180

Query: 194 GHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPD 253
           GH+S SPLAAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVER G+YVKPRDWN LISDPD
Sbjct: 181 GHSSGSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERAGQYVKPRDWNALISDPD 240

Query: 254 IVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNP 313
            VVIDVRNHYETRIGKFKRAVDPCT AFRDFP WVED+FQLPKSD +HSN+ VE + VNP
Sbjct: 241 TVVIDVRNHYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNP 300

Query: 314 EHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 373
           E NS PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV
Sbjct: 301 EQNSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 360

Query: 374 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARAR 433
           FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARAR
Sbjct: 361 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARAR 420

Query: 434 QRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           QRQFETWGIIGGPDKGRRPK+ D N  LPNS+
Sbjct: 421 QRQFETWGIIGGPDKGRRPKQSDKNATLPNSI 451

BLAST of Lcy13g010730 vs. NCBI nr
Match: KAG6600870.1 (Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 817.4 bits (2110), Expect = 6.3e-233
Identity = 404/453 (89.18%), Postives = 423/453 (93.38%), Query Frame = 0

Query: 14  MLRYRASPLLALGMVSPLL----SSSSSSLSSNPNLISFSPSFPILKPSPPLPSLLSSFL 73
           MLRYRA PLLALGMVSPLL    SSSSSS SSNPNLI+F+PSF +LKPSP LPSLLSSF 
Sbjct: 1   MLRYRAPPLLALGMVSPLLTSSSSSSSSSFSSNPNLITFNPSFLVLKPSPTLPSLLSSFH 60

Query: 74  QKPSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKK 133
           QKP+RSMTHS CF GPSPAVPIPTAC+A E++SV SLVVVSFYKFADFPDHAEFRSPLKK
Sbjct: 61  QKPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKK 120

Query: 134 LCEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIH 193
           LC+ELRVSGGIILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVSPEEEAIH
Sbjct: 121 LCQELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIH 180

Query: 194 HGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDP 253
           HGH+S SPLAAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVER G+YVKPRDWN LISDP
Sbjct: 181 HGHSSGSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERAGQYVKPRDWNALISDP 240

Query: 254 DIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVN 313
           D VVIDVRNHYETRIGKFKRAVDPCT AFRDFP WVED+FQLPKSD +HSN+ VE + VN
Sbjct: 241 DTVVIDVRNHYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVN 300

Query: 314 PEHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECF 373
           PE NS PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECF
Sbjct: 301 PEQNSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECF 360

Query: 374 VFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARA 433
           VFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARA
Sbjct: 361 VFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARA 420

Query: 434 RQRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           RQRQFETWGIIGGPDKGRRPK+ D N  LPNS+
Sbjct: 421 RQRQFETWGIIGGPDKGRRPKQSDKNATLPNSI 452

BLAST of Lcy13g010730 vs. NCBI nr
Match: XP_023513387.1 (rhodanese-like domain-containing protein 7 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 810.4 bits (2092), Expect = 7.7e-231
Identity = 402/452 (88.94%), Postives = 423/452 (93.58%), Query Frame = 0

Query: 14  MLRYRA-SPLLALGMVSPLL--SSSSSSLSSNPNLISFSPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA  P +ALGMVSPLL  SSSSSS SSNPNLI+F+PSFP+LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPPSVALGMVSPLLSSSSSSSSFSSNPNLITFNPSFPVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCF-GPSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKL 133
           KP+RSMTHS CF GPSPAVPIPTAC+  E++SV SLVVVSFYKFADFPDHAEFRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEVQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHH 193
           C+ELRVSGGIILAPEGINGSICG+RESVQRVLGFIESDERLKGLR+VETPVSPEEEAIHH
Sbjct: 121 CQELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRKVETPVSPEEEAIHH 180

Query: 194 GHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPD 253
           GH+SSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVERVG+YVKPRDWN LISDPD
Sbjct: 181 GHSSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNALISDPD 240

Query: 254 IVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNP 313
            VVIDVRNHYETRIGKFKRAVDPCT AFRDFP WVED+FQLPKSD +HS + VE + VNP
Sbjct: 241 TVVIDVRNHYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSIEEVEGKTVNP 300

Query: 314 EHNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 373
           E NS PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV
Sbjct: 301 EENSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFV 360

Query: 374 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARAR 433
           FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARAR
Sbjct: 361 FDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARAR 420

Query: 434 QRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 462
           QRQFETWGIIGGPDKGRRPK+ D N  LPNS+
Sbjct: 421 QRQFETWGIIGGPDKGRRPKQSDKNATLPNSI 451

BLAST of Lcy13g010730 vs. NCBI nr
Match: XP_022988667.1 (rhodanese-like domain-containing protein 7 [Cucurbita maxima])

HSP 1 Score: 799.7 bits (2064), Expect = 1.4e-227
Identity = 393/438 (89.73%), Postives = 413/438 (94.29%), Query Frame = 0

Query: 27  MVSPLLSSSS--SSLSSNPNLISFSPSFPILKPSPPLPSLLSSFLQKPSRSMTHSTCF-G 86
           MVSPLLSSSS  SS SSNPNLI+F+PSFPILKPSP LPSLLSSF QKPSRSMTHS CF G
Sbjct: 1   MVSPLLSSSSSYSSFSSNPNLITFNPSFPILKPSPTLPSLLSSFHQKPSRSMTHSKCFSG 60

Query: 87  PSPAVPIPTACQAAEIESVTSLVVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAP 146
           PSPAVPIP+AC+  E++S+ SLVVVSFYKFADFPDHAEFRSPLKKLC+ELRVSGGIILAP
Sbjct: 61  PSPAVPIPSACEVQEVDSM-SLVVVSFYKFADFPDHAEFRSPLKKLCQELRVSGGIILAP 120

Query: 147 EGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDA 206
           EGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGH+SSSPLAAGEDA
Sbjct: 121 EGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSSSPLAAGEDA 180

Query: 207 PFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDPDIVVIDVRNHYETRI 266
           PFRWDHVRVKLKKEIVTLGMP +SPVERVG+YVKPRDWN LISDPD VVIDVRNHYETRI
Sbjct: 181 PFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNTLISDPDTVVIDVRNHYETRI 240

Query: 267 GKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTG 326
           GKFKRAVDPCT AFRDFP WVED+FQLPKSD +HSN+ VE + VNPE NS PRVAMYCTG
Sbjct: 241 GKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEENSPPRVAMYCTG 300

Query: 327 GIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQG 386
           GIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQG
Sbjct: 301 GIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQG 360

Query: 387 TFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPD 446
           TFKLCYGCKQPVSDAD+ESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPD
Sbjct: 361 TFKLCYGCKQPVSDADIESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPD 420

Query: 447 KGRRPKEGDNNLNLPNSV 462
           KGRRPK+ D N  LPNS+
Sbjct: 421 KGRRPKQSDKNATLPNSI 437

BLAST of Lcy13g010730 vs. TAIR 10
Match: AT2G40760.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 575.9 bits (1483), Expect = 3.0e-164
Identity = 297/448 (66.29%), Postives = 334/448 (74.55%), Query Frame = 0

Query: 48  FSPSFPILK--PSPPLPSLLSSF-----------------LQKPSRSMTHSTCFGPSPAV 107
           F PS  +++   SPP P   S F                 LQ P +S  H     PS ++
Sbjct: 9   FPPSLAVVRMLSSPPPPHSHSPFSGGGVNSNSVGGNSKPELQFP-QSQPHKLSSSPSSSL 68

Query: 108 PIPTACQAA----------------EIESVT----SLVVVSFYKFADFPDHAEFRSPLKK 167
               AC  A                  ES+     SLVVVSFYKFADFP+HA+FR PLK 
Sbjct: 69  KSTVACSNAGAIRRSMATVSQAFSERTESIDSDLGSLVVVSFYKFADFPEHADFRKPLKD 128

Query: 168 LCEELRVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIH 227
           LCE+LRVSGGIILAPEGINGSICG RESV+ VL FI+ D RL GLRQVETPVSPE+EAIH
Sbjct: 129 LCEKLRVSGGIILAPEGINGSICGIRESVEEVLAFIQRDVRLNGLRQVETPVSPEQEAIH 188

Query: 228 HGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNVLISDP 287
           HGH+SSSPLAAGEDAPFRWDHVRVKLKKEIVTLG+P +SP+ERVG YV P +WN LISDP
Sbjct: 189 HGHSSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGIPSVSPIERVGTYVSPEEWNELISDP 248

Query: 288 DIVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVN 347
           + VVIDVRN YETRIGKFK AVDPCTTAFR+FP WVE++F L +  N+   +  +E+   
Sbjct: 249 ETVVIDVRNTYETRIGKFKGAVDPCTTAFRNFPSWVENQFALKQEGNETQAKVEKEDFSE 308

Query: 348 PEH--------NSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTE 407
             H         +LPR+AMYCTGGIRCEKASS LLS+GF+EVYHL+GGILKYLEEVPKTE
Sbjct: 309 ITHKEDKAEKPKTLPRIAMYCTGGIRCEKASSLLLSQGFEEVYHLKGGILKYLEEVPKTE 368

Query: 408 SLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSE 449
           SLWEGECFVFDKRVSVEHGL QGT KLCYGCKQP+SD DME+PE+EYGVSCP+CYS KSE
Sbjct: 369 SLWEGECFVFDKRVSVEHGLAQGTHKLCYGCKQPISDEDMEAPEYEYGVSCPYCYSKKSE 428

BLAST of Lcy13g010730 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 123.2 bits (308), Expect = 5.3e-28
Identity = 90/312 (28.85%), Postives = 149/312 (47.76%), Query Frame = 0

Query: 99  IESVTSLVVVSFYKFADFPDHAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQ 158
           IE      V+ +YK+   PD  E  S  +  C  L + G + L+P+G+N ++ G   +++
Sbjct: 14  IEDEEQYGVLLYYKYTSVPDLDELVSFYESSCNSLGLLGRVRLSPKGVNVTVGGKLTALE 73

Query: 159 RVLGFIESDERLKG----LRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKL 218
             +   +S+   +G    L     P++ ++ A   G TS S                +++
Sbjct: 74  EHIAAAKSNCLFEGTDFKLASCHHPLN-DKVAEECGFTSLS----------------IRV 133

Query: 219 KKEIVTLG-MPVISP--VERVGKYVKPRDWNVLI-------SDPDIVVIDVRNHYETRIG 278
            +E+VT    P + P  +   GK++   +++ ++        + ++V++D RN YETRIG
Sbjct: 134 VEELVTFSPCPPLKPPEISNAGKHLSAAEFHSVLQSANGKSENKELVLLDARNLYETRIG 193

Query: 279 KFK----RAVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMY 338
           KF+      +DP    + D P W++   +  K  N                     V MY
Sbjct: 194 KFESENVETLDPEIRQYSDLPTWIDQNAEKMKGKN---------------------VLMY 253

Query: 339 CTGGIRCEKASSYLLSK--GFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEH 389
           CTGGIRCE AS+Y+ SK  GF+  + L GGI +YLE+ P +   ++G+ FVFD R+SV  
Sbjct: 254 CTGGIRCEMASAYIRSKGAGFENTFQLYGGIQRYLEQFP-SGGFFKGKNFVFDHRISVGS 286

BLAST of Lcy13g010730 vs. TAIR 10
Match: AT1G17850.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 2.5e-22
Identity = 86/339 (25.37%), Postives = 140/339 (41.30%), Query Frame = 0

Query: 106 VVVSFYKFADFPD-HAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQRVLGFI 165
           +VV+FY+F    D  AE    L  L ++L + G I L  +GIN    G  +     + ++
Sbjct: 84  IVVNFYRFVSIGDPEAEIEKHLSFL-KDLNIRGRIYLNEQGINAQYSGPSKDALAYVEWL 143

Query: 166 ESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLG-- 225
           + D+R   L    +P                           +  ++++ K  +   G  
Sbjct: 144 KGDDRFSDLLVQMSPAMNRH---------------------AFPKLKLQNKPSLYEGGIS 203

Query: 226 -MPVISPVERVGKYVKPRDWNVLISD-------------PDIVVIDVRNHYETRIGKFKR 285
            +P++ P  R  K ++P +W   + D                +++DVRN YE  +G F+ 
Sbjct: 204 HLPLLDPPMR-AKPLEPSEWKRKLKDLTDDDEASPSNSGKSYILLDVRNGYEWDVGHFRG 263

Query: 286 AVDPCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCE 345
           A  P    FR+    + DE + P            +  +N +      + MYCTGGIRC+
Sbjct: 264 AHRPEVDCFRNTSFGLSDEKEAP-----------SDPLINVDKEKTD-ILMYCTGGIRCD 323

Query: 346 KASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLC 405
             S+ L  +GFK +Y L+GG+  YL+E    E  W G  FVFD R+S+          + 
Sbjct: 324 VYSTVLRQRGFKNLYTLKGGVSHYLKEEGTAE--WVGNLFVFDSRLSLPPAAYNDNV-VD 383

Query: 406 YGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERAR 428
                 V D    +P+     S   CY   S+ ++ R R
Sbjct: 384 KAVGDNVVDEAGRTPQTPVDTSFARCYLCNSQVQELRHR 384

BLAST of Lcy13g010730 vs. TAIR 10
Match: AT1G17850.2 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 3.3e-22
Identity = 87/336 (25.89%), Postives = 141/336 (41.96%), Query Frame = 0

Query: 106 VVVSFYKFADFPD-HAEFRSPLKKLCEELRVSGGIILAPEGINGSICGTRESVQRVLGFI 165
           +VV+FY+F    D  AE    L  L ++L + G I L  +GIN    G  +     + ++
Sbjct: 84  IVVNFYRFVSIGDPEAEIEKHLSFL-KDLNIRGRIYLNEQGINAQYSGPSKDALAYVEWL 143

Query: 166 ESDERLKGLRQVETPVSPEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMP 225
           + D+R   L    +P           +  + P    ++ P         ++ E     +P
Sbjct: 144 KGDDRFSDLLVQMSPAM---------NRHAFPKLKLQNKP-------SLVQYEGGISHLP 203

Query: 226 VISPVERVGKYVKPRDWNVLISD-------------PDIVVIDVRNHYETRIGKFKRAVD 285
           ++ P  R  K ++P +W   + D                +++DVRN YE  +G F+ A  
Sbjct: 204 LLDPPMR-AKPLEPSEWKRKLKDLTDDDEASPSNSGKSYILLDVRNGYEWDVGHFRGAHR 263

Query: 286 PCTTAFRDFPVWVEDEFQLPKSDNKHSNQGVEEEAVNPEHNSLPRVAMYCTGGIRCEKAS 345
           P    FR+    + DE + P            +  +N +      + MYCTGGIRC+  S
Sbjct: 264 PEVDCFRNTSFGLSDEKEAP-----------SDPLINVDKEKTD-ILMYCTGGIRCDVYS 323

Query: 346 SYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGC 405
           + L  +GFK +Y L+GG+  YL+E    E  W G  FVFD R+S+          +    
Sbjct: 324 TVLRQRGFKNLYTLKGGVSHYLKEEGTAE--WVGNLFVFDSRLSLPPAAYNDNV-VDKAV 383

Query: 406 KQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERAR 428
              V D    +P+     S   CY   S+ ++ R R
Sbjct: 384 GDNVVDEAGRTPQTPVDTSFARCYLCNSQVQELRHR 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1JPN04.2e-16366.29Rhodanese-like domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=ST... [more]
Q1GYV13.9e-8446.97tRNA uridine(34) hydroxylase OS=Methylobacillus flagellatus (strain KT / ATCC 51... [more]
Q92LX66.6e-8447.75tRNA uridine(34) hydroxylase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=tr... [more]
Q8YZS39.6e-8346.20tRNA uridine(34) hydroxylase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2... [more]
A6UD821.6e-8247.15tRNA uridine(34) hydroxylase OS=Sinorhizobium medicae (strain WSM419) OX=366394 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FRM32.3e-23389.38rhodanese-like domain-containing protein 7 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JDN96.6e-22889.73rhodanese-like domain-containing protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1CYE55.8e-22485.17rhodanese-like domain-containing protein 7 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A0A0KM632.3e-21784.25Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G223080 P... [more]
A0A1S4E1C58.6e-21284.04LOW QUALITY PROTEIN: rhodanese-like domain-containing protein 7 OS=Cucumis melo ... [more]
Match NameE-valueIdentityDescription
KAG7031505.11.1e-23489.82Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subs... [more]
XP_022942932.14.8e-23389.38rhodanese-like domain-containing protein 7 [Cucurbita moschata][more]
KAG6600870.16.3e-23389.18Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subs... [more]
XP_023513387.17.7e-23188.94rhodanese-like domain-containing protein 7 isoform X1 [Cucurbita pepo subsp. pep... [more]
XP_022988667.11.4e-22789.73rhodanese-like domain-containing protein 7 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G40760.13.0e-16466.29Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT1G09280.15.3e-2828.85CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas... [more]
AT1G17850.12.5e-2225.37Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT1G17850.23.3e-2225.89Rhodanese/Cell cycle control phosphatase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001763Rhodanese-like domainSMARTSM00450rhod_4coord: 236..358
e-value: 1.6E-19
score: 80.9
IPR001763Rhodanese-like domainPFAMPF00581Rhodanesecoord: 245..354
e-value: 8.4E-5
score: 23.1
IPR001763Rhodanese-like domainPROSITEPS50206RHODANESE_3coord: 246..361
score: 16.823586
NoneNo IPR availableGENE3D3.30.70.100coord: 94..219
e-value: 1.3E-24
score: 88.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..200
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 437..461
NoneNo IPR availablePANTHERPTHR43268:SF3THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2coord: 103..447
NoneNo IPR availableCDDcd01518RHOD_YceAcoord: 233..354
e-value: 2.33248E-50
score: 164.675
IPR040503tRNA uridine(34) hydroxylase, N-terminalPFAMPF17773UPF0176_Ncoord: 106..218
e-value: 5.2E-22
score: 78.0
IPR036873Rhodanese-like domain superfamilyGENE3D3.40.250.10coord: 220..397
e-value: 2.4E-49
score: 168.8
IPR036873Rhodanese-like domain superfamilySUPERFAMILY52821Rhodanese/Cell cycle control phosphatasecoord: 215..387
IPR020936tRNA uridine(34) hydroxylasePANTHERPTHR43268THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2coord: 103..447
IPR020936tRNA uridine(34) hydroxylaseHAMAPMF_00469TrhOcoord: 103..398
score: 39.799015

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g010730.1Lcy13g010730.1mRNA