Lcy13g005720 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g005720
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionGermin-like protein
LocationChr13: 4899193 .. 4899831 (+)
RNA-Seq ExpressionLcy13g005720
SyntenyLcy13g005720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCCAAATATATTGGTTCTGTTTTTCATCATCTTCCATGGATCTGTGACCTTGGCATCGGATCCCGATCCGGTACTCGACTTCTGCTTGCCGAGCGGGACGTCTGCAGGCAGTACTCATTCGTCGTGCAAGAACGCTTCCCTGGCTAAAGTAGAAGACTTCATTTTTTCTGGCATAAAGTTGCCTGGAAAGTTTAAGGGATCTGGCTTTTCATCCATAGCTGTTAATTCGAATGTCTTTCACGGTCTAAACACGCTCGGTATGTCGCTGGTTCGAGCGGATTTCGACGTTGGAGGTGTCAACGTTCCACATTTTCATCCTAGAGCAACTGAAGTTGCTGTTGTTCTTGAAGGAAAGATATATTCTGGGTTTGTTGATACCAATAACCAGATTTTTGCTAAGGTGATTGAGAAAGGTGAGGTCATGGTGTTTCCAAGAGGCTTAGTTCACTTCCAAATGAATGTTGGAGAAACTCCAGCTACAATTATAGGGAGTTTTGACAGCCAAAATCCTGGACTGATGAAAATTCCTTCTGCTGTTTTCGGATCCGGGATCAAAGAAGAGCTCTTGGAGAAGGCTTTCGGGTTGAGTCCCGACGAGCTCGCGAAGCTGAAAAAGAGGCATGCTCCCCATTGA

mRNA sequence

ATGCTTCCAAATATATTGGTTCTGTTTTTCATCATCTTCCATGGATCTGTGACCTTGGCATCGGATCCCGATCCGGTACTCGACTTCTGCTTGCCGAGCGGGACGTCTGCAGGCAGTACTCATTCGTCGTGCAAGAACGCTTCCCTGGCTAAAGTAGAAGACTTCATTTTTTCTGGCATAAAGTTGCCTGGAAAGTTTAAGGGATCTGGCTTTTCATCCATAGCTGTTAATTCGAATGTCTTTCACGGTCTAAACACGCTCGGTATGTCGCTGGTTCGAGCGGATTTCGACGTTGGAGGTGTCAACGTTCCACATTTTCATCCTAGAGCAACTGAAGTTGCTGTTGTTCTTGAAGGAAAGATATATTCTGGGTTTGTTGATACCAATAACCAGATTTTTGCTAAGGTGATTGAGAAAGGTGAGGTCATGGTGTTTCCAAGAGGCTTAGTTCACTTCCAAATGAATGTTGGAGAAACTCCAGCTACAATTATAGGGAGTTTTGACAGCCAAAATCCTGGACTGATGAAAATTCCTTCTGCTGTTTTCGGATCCGGGATCAAAGAAGAGCTCTTGGAGAAGGCTTTCGGGTTGAGTCCCGACGAGCTCGCGAAGCTGAAAAAGAGGCATGCTCCCCATTGA

Coding sequence (CDS)

ATGCTTCCAAATATATTGGTTCTGTTTTTCATCATCTTCCATGGATCTGTGACCTTGGCATCGGATCCCGATCCGGTACTCGACTTCTGCTTGCCGAGCGGGACGTCTGCAGGCAGTACTCATTCGTCGTGCAAGAACGCTTCCCTGGCTAAAGTAGAAGACTTCATTTTTTCTGGCATAAAGTTGCCTGGAAAGTTTAAGGGATCTGGCTTTTCATCCATAGCTGTTAATTCGAATGTCTTTCACGGTCTAAACACGCTCGGTATGTCGCTGGTTCGAGCGGATTTCGACGTTGGAGGTGTCAACGTTCCACATTTTCATCCTAGAGCAACTGAAGTTGCTGTTGTTCTTGAAGGAAAGATATATTCTGGGTTTGTTGATACCAATAACCAGATTTTTGCTAAGGTGATTGAGAAAGGTGAGGTCATGGTGTTTCCAAGAGGCTTAGTTCACTTCCAAATGAATGTTGGAGAAACTCCAGCTACAATTATAGGGAGTTTTGACAGCCAAAATCCTGGACTGATGAAAATTCCTTCTGCTGTTTTCGGATCCGGGATCAAAGAAGAGCTCTTGGAGAAGGCTTTCGGGTTGAGTCCCGACGAGCTCGCGAAGCTGAAAAAGAGGCATGCTCCCCATTGA

Protein sequence

MLPNILVLFFIIFHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGIKLPGKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFGSGIKEELLEKAFGLSPDELAKLKKRHAPH
Homology
BLAST of Lcy13g005720 vs. ExPASy Swiss-Prot
Match: Q9SR72 (Germin-like protein subfamily 3 member 2 OS=Arabidopsis thaliana OX=3702 GN=At3g10080 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 6.0e-64
Identity = 118/210 (56.19%), Postives = 155/210 (73.81%), Query Frame = 0

Query: 8   LFFIIFHGSVTLASDPDPVLDFCLPSGTSAG------STHSSCKNASLAKVEDFIFSGIK 67
           L  + F+   TLASDPDP+ DFC+P   ++       ST+  CKN+S    EDF+FSG+K
Sbjct: 12  LCLLCFNVCFTLASDPDPIQDFCIPKPVTSPYHDHHFSTNLPCKNSSEVTTEDFVFSGLK 71

Query: 68  LPGKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKI 127
             G F  +GF+++ V    F GLNTLG+S VRAD   G +N PH+HPRATEVA +++G++
Sbjct: 72  TAGNFTETGFATVPVGPENFPGLNTLGISFVRADLKPGSINPPHYHPRATEVAHLVKGRV 131

Query: 128 YSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAV 187
           YSGFVD+NN+++AKV+E+GE+MV+P+GLVHFQMNVG+  ATI+G  +SQNPG+ KIPS V
Sbjct: 132 YSGFVDSNNKVYAKVMEEGEMMVYPKGLVHFQMNVGDVTATIVGGLNSQNPGIQKIPSVV 191

Query: 188 FGSGIKEELLEKAFGLSPDELAKLKKRHAP 212
           FGSGI EELL KAFGLS  ++  LKKR  P
Sbjct: 192 FGSGINEELLMKAFGLSLKQIGTLKKRFDP 221

BLAST of Lcy13g005720 vs. ExPASy Swiss-Prot
Match: Q6ESF0 (Germin-like protein 2-4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0532500 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 5.1e-55
Identity = 105/214 (49.07%), Postives = 148/214 (69.16%), Query Frame = 0

Query: 6   LVLFFIIFHGSVTLASDPDPVLDFCLPSGTSAGSTHSS------CKNASLAKVEDFIFSG 65
           L+L   +   ++    DPD V DFC+P          +      C++ +     DF FSG
Sbjct: 8   LLLLLAVLLPAMAARGDPDAVQDFCVPDAGRGRPVELAMLPAYPCRSPANLTAGDFAFSG 67

Query: 66  IKLPGKFK-GSGFSSIAVNSNVFHGLNTLGMSLVRADFD-VGGVNVPHFHPRATEVAVVL 125
           ++  G F   +GF+ ++V    F GL+TLGMS  RAD    GGVN PH+HPRATE A+VL
Sbjct: 68  VRAAGNFSPETGFAGVSVTPAQFPGLHTLGMSFARADLSAAGGVNPPHYHPRATETALVL 127

Query: 126 EGKIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKI 185
            G++Y+GFVD+  ++FAKV+E+GEVMVFPR +VHFQ+NVG+TPAT+ G+F+S+NPG+++I
Sbjct: 128 AGRVYAGFVDSGGRLFAKVLEQGEVMVFPRAMVHFQLNVGDTPATVYGAFNSENPGIVRI 187

Query: 186 PSAVFGSGIKEELLEKAFGLSPDELAKLKKRHAP 212
           P+ VFGSGI+E +LE+AFGL+P EL +L+KR  P
Sbjct: 188 PATVFGSGIREAVLERAFGLTPAELRRLEKRFGP 221

BLAST of Lcy13g005720 vs. ExPASy Swiss-Prot
Match: Q6K5P8 (Putative germin-like protein 2-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0491800 PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.1e-38
Identity = 89/188 (47.34%), Postives = 118/188 (62.77%), Query Frame = 0

Query: 14  HGSVTLASDPDPVLDFCLPSGTSAGSTHS-SCKNASLAKVEDFIFSGIKLPGKFKGSGFS 73
           HG V +ASDP  + D C+    S    +  +CK+      EDF FSG+ + G       S
Sbjct: 23  HGGV-VASDPSHLQDLCVADKASTVRVNGVACKDGEDVAAEDFFFSGLHMAGNTTNKQGS 82

Query: 74  SI-AVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYSGFVDTN-- 133
           ++ AVN     GLNTLG+SL R D+ + G+N PH HPRATE+  VLEG +Y GFV +N  
Sbjct: 83  AVTAVNVAQVPGLNTLGISLARIDYALHGLNPPHTHPRATEILTVLEGSLYVGFVTSNPE 142

Query: 134 NQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFGS--GIK 193
           N++F KVI KG+V VFP+GLVHFQ N G T A  I +  SQNPG++ + +AVFGS   I 
Sbjct: 143 NKLFTKVINKGDVFVFPKGLVHFQFNYGTTDAVAIVALSSQNPGVITVANAVFGSKPSIT 202

Query: 194 EELLEKAF 196
           +++L KAF
Sbjct: 203 DDILAKAF 209

BLAST of Lcy13g005720 vs. ExPASy Swiss-Prot
Match: Q9FMA8 (Germin-like protein subfamily 1 member 11 OS=Arabidopsis thaliana OX=3702 GN=At5g38940 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.9e-38
Identity = 89/212 (41.98%), Postives = 127/212 (59.91%), Query Frame = 0

Query: 5   ILVLFFIIFHGSVTLASDPDPVLDFCLPSGTSAGSTHSS---CKNASLAKVEDFIFSGIK 64
           + VL  +     + +ASDP  + DFC+ + TSA     +   CK+  L   +DF FSG++
Sbjct: 9   LAVLSLLALTLPLAIASDPSQLQDFCVSANTSANGVFVNGKFCKDPKLVTADDFFFSGLQ 68

Query: 65  LPGKFKGS-GFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK 124
                    G +  AVN N   GLNTLG+SLVR D+ V G N PH HPRATE+ VV +G 
Sbjct: 69  TARPITSPVGSTVTAVNVNNLLGLNTLGISLVRIDYAVNGQNPPHTHPRATEILVVEQGT 128

Query: 125 IYSGFVDTN--NQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIP 184
           +  GFV +N  N++F+KV+ +G+V VFP GL+HFQ N+G+ PA    +  SQNPG++ I 
Sbjct: 129 LLVGFVTSNPDNRLFSKVLNEGDVFVFPEGLIHFQANIGKAPAVAFAALSSQNPGVITIA 188

Query: 185 SAVFGS--GIKEELLEKAFGLSPDELAKLKKR 209
           + VFG+   I   +L KAF L+P  +  L+ +
Sbjct: 189 NTVFGANPAINPTILAKAFQLNPRVVMDLQTK 220

BLAST of Lcy13g005720 vs. ExPASy Swiss-Prot
Match: Q9FMA6 (Putative germin-like protein subfamily 1 member 12 OS=Arabidopsis thaliana OX=3702 GN=At5g38960 PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.5e-38
Identity = 86/199 (43.22%), Postives = 121/199 (60.80%), Query Frame = 0

Query: 19  LASDPDPVLDFCLPSGTSAGSTHSS---CKNASLAKVEDFIFSGIKLPGKFKGS--GFSS 78
           +ASDP P+ DFC+   T A +   +   CK+  L   +DF FSG+      + S  G + 
Sbjct: 23  IASDPSPLQDFCIGVNTPANALFVNGKFCKDPKLVTADDFYFSGLDKARTTESSPVGSNV 82

Query: 79  IAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYSGFVDT--NNQ 138
             VN N   GLNTLG+SLVR D+ + G N PH HPRATE+ +V EG ++ GF  +   N+
Sbjct: 83  TTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSFPENR 142

Query: 139 IFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFGS--GIKEE 198
           +F K + KG+V VFP GL+HFQ+N+G+ PA    S  SQNPG++ I + +FGS   I   
Sbjct: 143 LFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVIIIGNTLFGSKPPIDPN 202

Query: 199 LLEKAFGLSPDELAKLKKR 209
           +L KAF L P  + +L+K+
Sbjct: 203 VLAKAFQLDPKVIIQLQKK 221

BLAST of Lcy13g005720 vs. ExPASy TrEMBL
Match: A0A5A7SZM1 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001990 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 9.8e-102
Identity = 190/209 (90.91%), Postives = 199/209 (95.22%), Query Frame = 0

Query: 5   ILVLFFII-FHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGIKLP 64
           +LVLFFII FHGS+TLASDPDPV DFCLP+ TSAGS+HSSCKN+SLA+VEDFIFSGIK P
Sbjct: 1   MLVLFFIILFHGSLTLASDPDPVFDFCLPNETSAGSSHSSCKNSSLARVEDFIFSGIKFP 60

Query: 65  GKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYS 124
           GKF GSGFSS AVNS VF GLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK+YS
Sbjct: 61  GKFNGSGFSSTAVNSKVFPGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKVYS 120

Query: 125 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFG 184
           GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGE PATIIGSFDSQNPGLMKIP+AVFG
Sbjct: 121 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGEIPATIIGSFDSQNPGLMKIPTAVFG 180

Query: 185 SGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           SGI+EELLEKAFGLSP ELAKLKKRHAPH
Sbjct: 181 SGIEEELLEKAFGLSPKELAKLKKRHAPH 209

BLAST of Lcy13g005720 vs. ExPASy TrEMBL
Match: A0A1S3BGN3 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103489391 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 9.8e-102
Identity = 190/209 (90.91%), Postives = 199/209 (95.22%), Query Frame = 0

Query: 5   ILVLFFII-FHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGIKLP 64
           +LVLFFII FHGS+TLASDPDPV DFCLP+ TSAGS+HSSCKN+SLA+VEDFIFSGIK P
Sbjct: 1   MLVLFFIILFHGSLTLASDPDPVFDFCLPNETSAGSSHSSCKNSSLARVEDFIFSGIKFP 60

Query: 65  GKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYS 124
           GKF GSGFSS AVNS VF GLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK+YS
Sbjct: 61  GKFNGSGFSSTAVNSKVFPGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKVYS 120

Query: 125 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFG 184
           GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGE PATIIGSFDSQNPGLMKIP+AVFG
Sbjct: 121 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGEIPATIIGSFDSQNPGLMKIPTAVFG 180

Query: 185 SGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           SGI+EELLEKAFGLSP ELAKLKKRHAPH
Sbjct: 181 SGIEEELLEKAFGLSPKELAKLKKRHAPH 209

BLAST of Lcy13g005720 vs. ExPASy TrEMBL
Match: A0A6J1JEZ1 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111483734 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.9e-99
Identity = 185/213 (86.85%), Postives = 198/213 (92.96%), Query Frame = 0

Query: 1   MLPNILVLFFIIFHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGI 60
           M+P +LVLF I+FHGS+T ASDPDPV DFCLPSG     THS CKN+SLAKVEDF+FSGI
Sbjct: 1   MVPKMLVLFLIVFHGSLTFASDPDPVFDFCLPSG-----THSPCKNSSLAKVEDFVFSGI 60

Query: 61  KLPGKF-KGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEG 120
           K PGKF  GSGFSSIAVNSNVF GLNTLGMS+VRADFDVGGVNVPHFHPRATEVAVVLEG
Sbjct: 61  KFPGKFINGSGFSSIAVNSNVFPGLNTLGMSMVRADFDVGGVNVPHFHPRATEVAVVLEG 120

Query: 121 KIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPS 180
           +IYSGFVDT+N+IFAKVIEKGEVMVFPRGLVHFQ+NVGETPATIIGSFDSQNPGLMKIP+
Sbjct: 121 RIYSGFVDTSNRIFAKVIEKGEVMVFPRGLVHFQLNVGETPATIIGSFDSQNPGLMKIPA 180

Query: 181 AVFGSGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           AVFGSGIKEELLEKAFGLSP+ELAKLKKRHA H
Sbjct: 181 AVFGSGIKEELLEKAFGLSPEELAKLKKRHASH 208

BLAST of Lcy13g005720 vs. ExPASy TrEMBL
Match: A0A6J1H0M4 (Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458497 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 7.7e-99
Identity = 187/213 (87.79%), Postives = 197/213 (92.49%), Query Frame = 0

Query: 1   MLPNILVLFFIIFHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGI 60
           M+P +LVLF IIFHGS+T ASDPDPV DFCLPS      THS CKN+SLAKVEDFIFSGI
Sbjct: 1   MVPKMLVLFLIIFHGSLTFASDPDPVFDFCLPS-----ETHSPCKNSSLAKVEDFIFSGI 60

Query: 61  KLPGKF-KGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEG 120
           K PGKF  GSGFSSIAVNSNVF GLNTLGMS+VRADFDVGGVNVPHFHPRATEVAVVLEG
Sbjct: 61  KFPGKFINGSGFSSIAVNSNVFPGLNTLGMSMVRADFDVGGVNVPHFHPRATEVAVVLEG 120

Query: 121 KIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPS 180
           KIYSGFVDT+N+IFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIP+
Sbjct: 121 KIYSGFVDTSNRIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPA 180

Query: 181 AVFGSGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           AVFGSGIKE+LLEKAFGLSP+ELAKLKKRHA H
Sbjct: 181 AVFGSGIKEDLLEKAFGLSPEELAKLKKRHASH 208

BLAST of Lcy13g005720 vs. ExPASy TrEMBL
Match: A0A6J1D8Y6 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111018710 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.8e-93
Identity = 175/212 (82.55%), Postives = 192/212 (90.57%), Query Frame = 0

Query: 1   MLPNILVLFFII-FHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSG 60
           M+P +++L FII FHGS+T+ASDPDP+ DFCLPSGT       SCKN+S A VEDF+F G
Sbjct: 1   MIPKMVILLFIIFFHGSMTMASDPDPIFDFCLPSGT------PSCKNSSQANVEDFVFYG 60

Query: 61  IKLPGKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEG 120
           IK PGKF  SGFSS  VN++VF GLNTLG+S+VRADFDVGGVNVPHFHPRATEVAVVLEG
Sbjct: 61  IKFPGKFNRSGFSSTPVNADVFPGLNTLGISMVRADFDVGGVNVPHFHPRATEVAVVLEG 120

Query: 121 KIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPS 180
           KIYSGFVDTN+QIFAKVIEKGEVMVFPRGLVHFQMNVGETPATI+GSFDSQNPGLMKIP+
Sbjct: 121 KIYSGFVDTNSQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATILGSFDSQNPGLMKIPA 180

Query: 181 AVFGSGIKEELLEKAFGLSPDELAKLKKRHAP 212
           AVFGSGIKEELLEKAFGLSP+ELAKLKKRHAP
Sbjct: 181 AVFGSGIKEELLEKAFGLSPEELAKLKKRHAP 206

BLAST of Lcy13g005720 vs. NCBI nr
Match: XP_038891467.1 (germin-like protein subfamily 3 member 2 [Benincasa hispida])

HSP 1 Score: 396.7 bits (1018), Expect = 1.2e-106
Identity = 199/213 (93.43%), Postives = 205/213 (96.24%), Query Frame = 0

Query: 1   MLPNILVLFFI-IFHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSG 60
           MLP ILV FFI IFHGS+TLASDPDPV DFCLPSGTSAGS+HSSCKN+SLAKVEDFIFSG
Sbjct: 1   MLPKILVSFFIVIFHGSLTLASDPDPVFDFCLPSGTSAGSSHSSCKNSSLAKVEDFIFSG 60

Query: 61  IKLPGKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEG 120
           IK PGKF GSGFSSIAVNSNVF GLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEG
Sbjct: 61  IKFPGKFNGSGFSSIAVNSNVFPGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEG 120

Query: 121 KIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPS 180
           KIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIP+
Sbjct: 121 KIYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPA 180

Query: 181 AVFGSGIKEELLEKAFGLSPDELAKLKKRHAPH 213
            VFGSGIK+ELLEKAFGLSP+ELAKLKKRHAPH
Sbjct: 181 VVFGSGIKDELLEKAFGLSPEELAKLKKRHAPH 213

BLAST of Lcy13g005720 vs. NCBI nr
Match: XP_008446760.1 (PREDICTED: germin-like protein subfamily 3 member 2 [Cucumis melo] >KAA0034655.1 germin-like protein subfamily 3 member 2 [Cucumis melo var. makuwa] >TYK09206.1 germin-like protein subfamily 3 member 2 [Cucumis melo var. makuwa])

HSP 1 Score: 379.4 bits (973), Expect = 2.0e-101
Identity = 190/209 (90.91%), Postives = 199/209 (95.22%), Query Frame = 0

Query: 5   ILVLFFII-FHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGIKLP 64
           +LVLFFII FHGS+TLASDPDPV DFCLP+ TSAGS+HSSCKN+SLA+VEDFIFSGIK P
Sbjct: 1   MLVLFFIILFHGSLTLASDPDPVFDFCLPNETSAGSSHSSCKNSSLARVEDFIFSGIKFP 60

Query: 65  GKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYS 124
           GKF GSGFSS AVNS VF GLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK+YS
Sbjct: 61  GKFNGSGFSSTAVNSKVFPGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKVYS 120

Query: 125 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFG 184
           GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGE PATIIGSFDSQNPGLMKIP+AVFG
Sbjct: 121 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGEIPATIIGSFDSQNPGLMKIPTAVFG 180

Query: 185 SGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           SGI+EELLEKAFGLSP ELAKLKKRHAPH
Sbjct: 181 SGIEEELLEKAFGLSPKELAKLKKRHAPH 209

BLAST of Lcy13g005720 vs. NCBI nr
Match: XP_004135363.1 (germin-like protein subfamily 3 member 2 [Cucumis sativus])

HSP 1 Score: 378.3 bits (970), Expect = 4.5e-101
Identity = 190/209 (90.91%), Postives = 198/209 (94.74%), Query Frame = 0

Query: 5   ILVLFFII-FHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGIKLP 64
           +LVLFFII FH S+TLASDPDPV DFCLP+ TSAGS+HS CKN+SLA+VEDFIFSGIK P
Sbjct: 1   MLVLFFIILFHRSLTLASDPDPVFDFCLPNETSAGSSHSLCKNSSLARVEDFIFSGIKFP 60

Query: 65  GKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYS 124
           GKF GSGFSSIAVNS VF GLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK+YS
Sbjct: 61  GKFNGSGFSSIAVNSKVFPGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKVYS 120

Query: 125 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFG 184
           GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGE PATIIGSFDSQNPGLMKIPSAVFG
Sbjct: 121 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGEIPATIIGSFDSQNPGLMKIPSAVFG 180

Query: 185 SGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           SGIKEELLEKAFGLSP+EL KLKKRHAPH
Sbjct: 181 SGIKEELLEKAFGLSPEELVKLKKRHAPH 209

BLAST of Lcy13g005720 vs. NCBI nr
Match: KAE8648820.1 (hypothetical protein Csa_008694 [Cucumis sativus])

HSP 1 Score: 378.3 bits (970), Expect = 4.5e-101
Identity = 190/209 (90.91%), Postives = 198/209 (94.74%), Query Frame = 0

Query: 5   ILVLFFII-FHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGIKLP 64
           +LVLFFII FH S+TLASDPDPV DFCLP+ TSAGS+HS CKN+SLA+VEDFIFSGIK P
Sbjct: 21  MLVLFFIILFHRSLTLASDPDPVFDFCLPNETSAGSSHSLCKNSSLARVEDFIFSGIKFP 80

Query: 65  GKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYS 124
           GKF GSGFSSIAVNS VF GLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK+YS
Sbjct: 81  GKFNGSGFSSIAVNSKVFPGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKVYS 140

Query: 125 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFG 184
           GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGE PATIIGSFDSQNPGLMKIPSAVFG
Sbjct: 141 GFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGEIPATIIGSFDSQNPGLMKIPSAVFG 200

Query: 185 SGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           SGIKEELLEKAFGLSP+EL KLKKRHAPH
Sbjct: 201 SGIKEELLEKAFGLSPEELVKLKKRHAPH 229

BLAST of Lcy13g005720 vs. NCBI nr
Match: KAG6601221.1 (Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032016.1 Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 375.9 bits (964), Expect = 2.2e-100
Identity = 188/212 (88.68%), Postives = 197/212 (92.92%), Query Frame = 0

Query: 1   MLPNILVLFFIIFHGSVTLASDPDPVLDFCLPSGTSAGSTHSSCKNASLAKVEDFIFSGI 60
           M+P +LVLF IIFHGS+ LASDPDPV DFCLPSG     THS CKN SLAKVEDFIFSGI
Sbjct: 1   MVPKMLVLFLIIFHGSLALASDPDPVFDFCLPSG-----THSPCKNTSLAKVEDFIFSGI 60

Query: 61  KLPGKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK 120
           K PGKF GSGFSSIAVNSNVF GLNTLGMS+VRADFDVGGVNVPHFHPRATEVAVVLEGK
Sbjct: 61  KFPGKFNGSGFSSIAVNSNVFPGLNTLGMSMVRADFDVGGVNVPHFHPRATEVAVVLEGK 120

Query: 121 IYSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSA 180
           IYSGFVDT+N+IFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIP+A
Sbjct: 121 IYSGFVDTSNRIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPAA 180

Query: 181 VFGSGIKEELLEKAFGLSPDELAKLKKRHAPH 213
           VFGSGIKE+LLEKAFGLSP+ELAKLKKRHA H
Sbjct: 181 VFGSGIKEDLLEKAFGLSPEELAKLKKRHASH 207

BLAST of Lcy13g005720 vs. TAIR 10
Match: AT3G10080.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 4.2e-65
Identity = 118/210 (56.19%), Postives = 155/210 (73.81%), Query Frame = 0

Query: 8   LFFIIFHGSVTLASDPDPVLDFCLPSGTSAG------STHSSCKNASLAKVEDFIFSGIK 67
           L  + F+   TLASDPDP+ DFC+P   ++       ST+  CKN+S    EDF+FSG+K
Sbjct: 12  LCLLCFNVCFTLASDPDPIQDFCIPKPVTSPYHDHHFSTNLPCKNSSEVTTEDFVFSGLK 71

Query: 68  LPGKFKGSGFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKI 127
             G F  +GF+++ V    F GLNTLG+S VRAD   G +N PH+HPRATEVA +++G++
Sbjct: 72  TAGNFTETGFATVPVGPENFPGLNTLGISFVRADLKPGSINPPHYHPRATEVAHLVKGRV 131

Query: 128 YSGFVDTNNQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAV 187
           YSGFVD+NN+++AKV+E+GE+MV+P+GLVHFQMNVG+  ATI+G  +SQNPG+ KIPS V
Sbjct: 132 YSGFVDSNNKVYAKVMEEGEMMVYPKGLVHFQMNVGDVTATIVGGLNSQNPGIQKIPSVV 191

Query: 188 FGSGIKEELLEKAFGLSPDELAKLKKRHAP 212
           FGSGI EELL KAFGLS  ++  LKKR  P
Sbjct: 192 FGSGINEELLMKAFGLSLKQIGTLKKRFDP 221

BLAST of Lcy13g005720 vs. TAIR 10
Match: AT5G38940.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.4e-39
Identity = 89/212 (41.98%), Postives = 127/212 (59.91%), Query Frame = 0

Query: 5   ILVLFFIIFHGSVTLASDPDPVLDFCLPSGTSAGSTHSS---CKNASLAKVEDFIFSGIK 64
           + VL  +     + +ASDP  + DFC+ + TSA     +   CK+  L   +DF FSG++
Sbjct: 9   LAVLSLLALTLPLAIASDPSQLQDFCVSANTSANGVFVNGKFCKDPKLVTADDFFFSGLQ 68

Query: 65  LPGKFKGS-GFSSIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGK 124
                    G +  AVN N   GLNTLG+SLVR D+ V G N PH HPRATE+ VV +G 
Sbjct: 69  TARPITSPVGSTVTAVNVNNLLGLNTLGISLVRIDYAVNGQNPPHTHPRATEILVVEQGT 128

Query: 125 IYSGFVDTN--NQIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIP 184
           +  GFV +N  N++F+KV+ +G+V VFP GL+HFQ N+G+ PA    +  SQNPG++ I 
Sbjct: 129 LLVGFVTSNPDNRLFSKVLNEGDVFVFPEGLIHFQANIGKAPAVAFAALSSQNPGVITIA 188

Query: 185 SAVFGS--GIKEELLEKAFGLSPDELAKLKKR 209
           + VFG+   I   +L KAF L+P  +  L+ +
Sbjct: 189 NTVFGANPAINPTILAKAFQLNPRVVMDLQTK 220

BLAST of Lcy13g005720 vs. TAIR 10
Match: AT5G38960.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 160.2 bits (404), Expect = 1.8e-39
Identity = 86/199 (43.22%), Postives = 121/199 (60.80%), Query Frame = 0

Query: 19  LASDPDPVLDFCLPSGTSAGSTHSS---CKNASLAKVEDFIFSGIKLPGKFKGS--GFSS 78
           +ASDP P+ DFC+   T A +   +   CK+  L   +DF FSG+      + S  G + 
Sbjct: 21  IASDPSPLQDFCIGVNTPANALFVNGKFCKDPKLVTADDFYFSGLDKARTTESSPVGSNV 80

Query: 79  IAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYSGFVDT--NNQ 138
             VN N   GLNTLG+SLVR D+ + G N PH HPRATE+ +V EG ++ GF  +   N+
Sbjct: 81  TTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSFPENR 140

Query: 139 IFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFGS--GIKEE 198
           +F K + KG+V VFP GL+HFQ+N+G+ PA    S  SQNPG++ I + +FGS   I   
Sbjct: 141 LFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVIIIGNTLFGSKPPIDPN 200

Query: 199 LLEKAFGLSPDELAKLKKR 209
           +L KAF L P  + +L+K+
Sbjct: 201 VLAKAFQLDPKVIIQLQKK 219

BLAST of Lcy13g005720 vs. TAIR 10
Match: AT5G38930.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 4.4e-38
Identity = 85/200 (42.50%), Postives = 119/200 (59.50%), Query Frame = 0

Query: 17  VTLASDPDPVLDFCLPSGTSAGSTHSS---CKNASLAKVEDFIFSGIKLPGKFKGS-GFS 76
           +T+ASDP  + DFC+ + +SA     +   CK+  L   +DF F G++         G +
Sbjct: 21  LTIASDPSQLQDFCVSANSSANGVFVNGKFCKDPKLVTADDFFFPGLQTARPITSPVGST 80

Query: 77  SIAVNSNVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYSGFVDTN--N 136
             AVN N   GLNTLG+SLVR D+ V G N PH HPRATE+ VV  G +  GFV +N  N
Sbjct: 81  VTAVNVNNLLGLNTLGISLVRIDYAVDGQNPPHTHPRATEILVVELGTLLVGFVTSNPDN 140

Query: 137 QIFAKVIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFGS--GIKE 196
           ++F KV+ +G+V VFP GL+HFQ N+G+ PA    +  SQNPG++ I   VFG+   I  
Sbjct: 141 RLFTKVLNEGDVFVFPEGLIHFQANIGKAPAVAFAALSSQNPGVITIAPTVFGANPAINP 200

Query: 197 ELLEKAFGLSPDELAKLKKR 209
            +L KAF + P  +  L+ +
Sbjct: 201 NILAKAFQVDPRVVMDLQTK 220

BLAST of Lcy13g005720 vs. TAIR 10
Match: AT3G05950.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 9.8e-38
Identity = 88/195 (45.13%), Postives = 115/195 (58.97%), Query Frame = 0

Query: 22  DPDPVLDFCLPSGTSAG--STHSSCKNASLAKVEDFIFSGIKLPGK-FKGSGFSSIAVNS 81
           DP P+ DFC+    ++G       CK+    K EDF  SG+ + G      G +   VN 
Sbjct: 26  DPSPLQDFCVAVDDASGVFVNGKFCKDPKYVKAEDFFTSGLNIAGNTINRVGSNVTNVNV 85

Query: 82  NVFHGLNTLGMSLVRADFDVGGVNVPHFHPRATEVAVVLEGKIYSGFVDT---NNQIFAK 141
           +   GLNTLG+SLVR DF  GG N PH HPRATE+ VV+EG +  GFV +   NN++F+K
Sbjct: 86  DKIPGLNTLGVSLVRIDFAPGGQNPPHTHPRATEILVVVEGTLLVGFVTSNQDNNRLFSK 145

Query: 142 VIEKGEVMVFPRGLVHFQMNVGETPATIIGSFDSQNPGLMKIPSAVFGS--GIKEELLEK 201
           V+  G+V VFP G++HFQ+NVG T A       SQNPG + I  AVFGS   I  E+L K
Sbjct: 146 VLYPGDVFVFPIGMIHFQVNVGRTNAVAFAGLGSQNPGTITIADAVFGSKPSIMPEILAK 205

Query: 202 AFGLSPDELAKLKKR 209
           AF L  + +  L+ R
Sbjct: 206 AFQLDVNVVKYLEAR 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SR726.0e-6456.19Germin-like protein subfamily 3 member 2 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q6ESF05.1e-5549.07Germin-like protein 2-4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0532500... [more]
Q6K5P81.1e-3847.34Putative germin-like protein 2-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0... [more]
Q9FMA81.9e-3841.98Germin-like protein subfamily 1 member 11 OS=Arabidopsis thaliana OX=3702 GN=At5... [more]
Q9FMA62.5e-3843.22Putative germin-like protein subfamily 1 member 12 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A5A7SZM19.8e-10290.91Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G... [more]
A0A1S3BGN39.8e-10290.91Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103489391 PE=3 SV=1[more]
A0A6J1JEZ15.9e-9986.85Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111483734 PE=3 SV=1[more]
A0A6J1H0M47.7e-9987.79Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458497 PE=3 SV=1[more]
A0A6J1D8Y62.8e-9382.55Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111018710 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038891467.11.2e-10693.43germin-like protein subfamily 3 member 2 [Benincasa hispida][more]
XP_008446760.12.0e-10190.91PREDICTED: germin-like protein subfamily 3 member 2 [Cucumis melo] >KAA0034655.1... [more]
XP_004135363.14.5e-10190.91germin-like protein subfamily 3 member 2 [Cucumis sativus][more]
KAE8648820.14.5e-10190.91hypothetical protein Csa_008694 [Cucumis sativus][more]
KAG6601221.12.2e-10088.68Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
AT3G10080.14.2e-6556.19RmlC-like cupins superfamily protein [more]
AT5G38940.11.4e-3941.98RmlC-like cupins superfamily protein [more]
AT5G38960.11.8e-3943.22RmlC-like cupins superfamily protein [more]
AT5G38930.14.4e-3842.50RmlC-like cupins superfamily protein [more]
AT3G05950.19.8e-3845.13RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001929GerminPRINTSPR00325GERMINcoord: 135..155
score: 56.92
coord: 105..125
score: 55.59
coord: 168..183
score: 44.48
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 58..203
e-value: 1.6E-40
score: 150.6
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 63..200
e-value: 5.9E-39
score: 133.1
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 21..211
e-value: 1.0E-62
score: 213.1
NoneNo IPR availablePIRSRPIRSR617774-2PIRSR617774-2coord: 89..208
e-value: 7.8E-7
score: 26.1
NoneNo IPR availablePANTHERPTHR31238:SF114GERMIN-LIKE PROTEINcoord: 5..210
NoneNo IPR availablePANTHERPTHR31238GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3coord: 5..210
NoneNo IPR availableCDDcd02241cupin_OxOxcoord: 22..208
e-value: 2.14498E-74
score: 221.315
IPR019780Germin, manganese binding sitePROSITEPS00725GERMINcoord: 100..113
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 54..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g005720.1Lcy13g005720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030145 manganese ion binding