Lcy12g020460 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g020460
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Calmodulin
Location	Chr12: 47709485 .. 47710511 (-)
RNA-Seq Expression	Lcy12g020460
Synteny	Lcy12g020460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCTCACCAAAATACCAATGGCCATTTCAACCATTTCCATTCCCTTAAAATGGTTTTCCAAAACAAATTTCAAGCTCAATTTCCCTCGCCTCCACTCCAAGCACAAACCCACCACGTCGTCTAAACCTCCTCCACCGCCCCTCACGGCGGCGCCACCATTTGCCGAAGCAGCCTTCCGCCACATCGATACTGACGGAGACGGCAAAATCTCCAGCGACGAGCTCCGATCCTACTTCGTGTCGATCGGTGAGTCCATGACAGGCGACGATGCTCGAACTGTGATTGGAGACTACGATGGCGATGGAGACGAGCTACTCGAGCTTCGAGATTTCGAGCGATTGATAAAGGCGGAGGAGGAGGAAGAAGAGAAGGAGGATTTGAAAATGGCTTTTGAGATGTTTGAAGTTGAGAAGGGATGTGGATTCATCGGACCGGCGGGATTGCAGAAGATGTTTGGGCGGCTTGGGTACGTGAAATCGAAAGAGGAGTGCATGGCTATGATCGGAGTGTTTGACGTTGACGGCGACGGCGTAATTGATTATCGTGAATTTCTCAGAATGATGACGGCCTAATTTAACGTGTGAATAAATATATAATATCTTCTAATTGCTGTCGTGAAGCTGGATTTTTTTTTTTTTTTCTTTTTTTCATGTTAATTAAAAGTGTGGTTGTAAATCTGAGAGGCGTTTGGTTAGTTAAATGTGTCTGCAAAATTTATGTTCTTTTTTCATTTGAGGGAAGTTGAAGTTGTTGGTTTGATATTATGGAGTAATTTGTAACTGTCGAAAAACTAAAAAACAATGTGATGTCGGATCGGGACGCAGGAACTTCCGGTTGGCGCGGGAGACTTATGTTTTGACTTTTGACGTGTATCCGATGTGGATGATGACTGCGATTGTCGCTATCCTTATAAACTGCTTGGCGTTGACAATAGTCAACTCTAGTATGTTTAGTCAATGTTTATTTAGCTCTAGTGGCATACCGGTCCCTACATATGATCGTTAAAATCTTTGACAACAAAATGAG

mRNA sequence

AAGCTCACCAAAATACCAATGGCCATTTCAACCATTTCCATTCCCTTAAAATGGTTTTCCAAAACAAATTTCAAGCTCAATTTCCCTCGCCTCCACTCCAAGCACAAACCCACCACGTCGTCTAAACCTCCTCCACCGCCCCTCACGGCGGCGCCACCATTTGCCGAAGCAGCCTTCCGCCACATCGATACTGACGGAGACGGCAAAATCTCCAGCGACGAGCTCCGATCCTACTTCGTGTCGATCGGTGAGTCCATGACAGGCGACGATGCTCGAACTGTGATTGGAGACTACGATGGCGATGGAGACGAGCTACTCGAGCTTCGAGATTTCGAGCGATTGATAAAGGCGGAGGAGGAGGAAGAAGAGAAGGAGGATTTGAAAATGGCTTTTGAGATGTTTGAAGTTGAGAAGGGATGTGGATTCATCGGACCGGCGGGATTGCAGAAGATGTTTGGGCGGCTTGGGTACGTGAAATCGAAAGAGGAGTGCATGGCTATGATCGGAGTGTTTGACGTTGACGGCGACGGCGTAATTGATTATCGTGAATTTCTCAGAATGATGACGGCCTAATTTAACGTGTGAATAAATATATAATATCTTCTAATTGCTGTCGTGAAGCTGGATTTTTTTTTTTTTTTCTTTTTTTCATGTTAATTAAAAGTGTGGTTGTAAATCTGAGAGGCGTTTGGTTAGTTAAATGTGTCTGCAAAATTTATGTTCTTTTTTCATTTGAGGGAAGTTGAAGTTGTTGGTTTGATATTATGGAGTAATTTGTAACTGTCGAAAAACTAAAAAACAATGTGATGTCGGATCGGGACGCAGGAACTTCCGGTTGGCGCGGGAGACTTATGTTTTGACTTTTGACGTGTATCCGATGTGGATGATGACTGCGATTGTCGCTATCCTTATAAACTGCTTGGCGTTGACAATAGTCAACTCTAGTATGTTTAGTCAATGTTTATTTAGCTCTAGTGGCATACCGGTCCCTACATATGATCGTTAAAATCTTTGACAACAAAATGAG

Coding sequence (CDS)

ATGGCCATTTCAACCATTTCCATTCCCTTAAAATGGTTTTCCAAAACAAATTTCAAGCTCAATTTCCCTCGCCTCCACTCCAAGCACAAACCCACCACGTCGTCTAAACCTCCTCCACCGCCCCTCACGGCGGCGCCACCATTTGCCGAAGCAGCCTTCCGCCACATCGATACTGACGGAGACGGCAAAATCTCCAGCGACGAGCTCCGATCCTACTTCGTGTCGATCGGTGAGTCCATGACAGGCGACGATGCTCGAACTGTGATTGGAGACTACGATGGCGATGGAGACGAGCTACTCGAGCTTCGAGATTTCGAGCGATTGATAAAGGCGGAGGAGGAGGAAGAAGAGAAGGAGGATTTGAAAATGGCTTTTGAGATGTTTGAAGTTGAGAAGGGATGTGGATTCATCGGACCGGCGGGATTGCAGAAGATGTTTGGGCGGCTTGGGTACGTGAAATCGAAAGAGGAGTGCATGGCTATGATCGGAGTGTTTGACGTTGACGGCGACGGCGTAATTGATTATCGTGAATTTCTCAGAATGATGACGGCCTAA

Protein sequence

MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPPPLTAAPPFAEAAFRHIDTDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREFLRMMTA

Homology

[Close]

BLAST of Lcy12g020460 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.3e-26
Identity = 77/191 (40.31%), Postives = 104/191 (54.45%), Query Frame = 0


Query: 11  KWFSKTNFKLNFPRLHSKHKP-TTSSKPPPPPLT------------AAPPFAEAAFRHID 70
           KWFS    KLN    + +  P + SS     P +            A+       F H D
Sbjct: 14  KWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVFSHFD 73

Query: 71  TDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDF-----ERLIKAE 130
           +DGDGKIS+ ELR YF S+GE ++ + A+  I + D D D  L   DF      R +  +
Sbjct: 74  SDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRDLYGD 133

Query: 131 EEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGV 184
            E +   +LK AFEMFEVEKG G I P GLQKM  +LG  ++  EC AMI  +D+DG+G+
Sbjct: 134 GEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNGI 193

[Close]

BLAST of Lcy12g020460 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.2e-20
Identity = 50/133 (37.59%), Postives = 86/133 (64.66%), Query Frame = 0


Query: 50  EAAFRHIDTDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLI 109
           EA F ++D + DG+IS +EL+  F+++GE ++ ++A   +   D DGD +L+  +F +LI
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 110 KAEEEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDG 169
           K ++EEE+K +LK AF ++ + +G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 170 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Lcy12g020460 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.7e-19
Identity = 62/146 (42.47%), Postives = 89/146 (60.96%), Query Frame = 0


Query: 44  AAPPFAEAAFRHIDTDGDGKISSDELRSYF-VSIGESMTGDDARTVIGDYDGDGDELLEL 103
           A+P  + A F   D DGDG+IS+ ELR     ++GE ++ ++A  ++   D DGD LL  
Sbjct: 7   ASPCESSALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCE 66

Query: 104 RDFERLIKAEEEEEEKE----DLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEEC 163
            +F RL++A E EEE E     L+ AF M+E+E G G I P  L++M  RLG  +  ++C
Sbjct: 67  AEFVRLVQAAEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDC 126

Query: 164 MAMIGVFDVDGDGVIDYREFLRMMTA 185
            AMI  FD++GDGV+ + EF  MM A
Sbjct: 127 RAMICRFDLNGDGVLSFDEFKIMMNA 151

[Close]

BLAST of Lcy12g020460 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.5e-18
Identity = 60/147 (40.82%), Postives = 83/147 (56.46%), Query Frame = 0


Query: 42  LTAAPPFAEAAFRHIDTDGDGKISSDELRSYF-VSIGESMTGDDARTVIGDYDGDGDELL 101
           + AA       F   D D DGKIS+ ELR     ++GE M+ ++A  ++   D D D LL
Sbjct: 1   MVAATAEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLL 60

Query: 102 ELRDFERL---IKAEEEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEE 161
           +  +F +L   ++  +EEE    L  AF M+E+E G G I PA L++M  +LG  +  EE
Sbjct: 61  DEEEFTKLAVQLEMGDEEERCRGLMEAFRMYEME-GEGRITPASLKRMLSKLGSHQGIEE 120

Query: 162 CMAMIGVFDVDGDGVIDYREFLRMMTA 185
           C  MI  FD+DGDGVI + EF  MM A
Sbjct: 121 CQTMICRFDLDGDGVISFEEFKIMMDA 146

[Close]

BLAST of Lcy12g020460 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-18
Identity = 50/136 (36.76%), Postives = 82/136 (60.29%), Query Frame = 0


Query: 50  EAAFRHIDTDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLI 109
           EA F ++D + DG+IS++EL+  F ++GE M+ ++A   +   D DGD +L++ +F  LI
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 110 KAEE---EEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFD 169
           K  +   EEE+K  +  AF M+ +  G   I P  L+ M  +LG  ++ ++C  MI  FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMY-IADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 170 VDGDGVIDYREFLRMM 183
           ++ DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

[Close]

BLAST of Lcy12g020460 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.6e-74
Identity = 148/185 (80.00%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPP--PLTAAPPFAEAAFRHIDT 60
           MA+S IS P KWFS TNFKLNFPRL  KHKPT S+ PPPP   LT APP AEAAFRH+D 
Sbjct: 15  MALSPISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDA 74

Query: 61  DGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYF S+GE MT DDAR++I ++DGDGD LLEL DFERL+K   EEEEK
Sbjct: 75  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG-GEEEEK 134

Query: 121 EDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLKMAFEMFE EKGCGFIGP GLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 135 EDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 194

Query: 181 LRMMT 184
           LRMMT
Sbjct: 195 LRMMT 198

[Close]

BLAST of Lcy12g020460 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 5.4e-72
Identity = 144/184 (78.26%), Postives = 156/184 (84.78%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPP-PLTAAPPFAEAAFRHIDTD 60
           MA+S IS P KWFS TN KLN PRL  KHKPT S+ PPPP  LT APP AEAAFRH+D D
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLTPAPPLAEAAFRHLDAD 60

Query: 61  GDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEKE 120
           GDGKIS DEL+SYF S+GE MT DDAR++I ++DGDGD LLEL DFERL+K   E+EEKE
Sbjct: 61  GDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG-GEQEEKE 120

Query: 121 DLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREFL 180
           DLK AFEMFE EKGCGFIGPAGLQKMFGRLGYVKS EECMAMIGVFDVDG+GVIDYREFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFL 180

Query: 181 RMMT 184
           RMMT
Sbjct: 181 RMMT 183

[Close]

BLAST of Lcy12g020460 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.7e-71
Identity = 145/183 (79.23%), Postives = 154/183 (84.15%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPPPLTAAPPFAEAAFRHIDTDG 60
           MAISTI+IP KWF+KTNFKLNFP LHSKHKP  SSKPP P     PP AE AFRH+D DG
Sbjct: 1   MAISTIAIPFKWFTKTNFKLNFPLLHSKHKP-ISSKPPTP-----PPLAEVAFRHLDIDG 60

Query: 61  DGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEKED 120
           DGKIS DELRSYF SIGE MT D A++VIGD+D DGDELLEL DFERL+K EEEE+E ED
Sbjct: 61  DGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQE-ED 120

Query: 121 LKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREFLR 180
           LK AFEMFE EKGCGFIG  GLQKMFGRLGYVKSKE+CM MI VFDVDGDGVIDY EFLR
Sbjct: 121 LKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLR 176

Query: 181 MMT 184
           MMT
Sbjct: 181 MMT 176

[Close]

BLAST of Lcy12g020460 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.7e-71
Identity = 145/183 (79.23%), Postives = 154/183 (84.15%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPPPLTAAPPFAEAAFRHIDTDG 60
           MAISTI+IP KWF+KTNFKLNFP LHSKHKP  SSKPP P     PP AE AFRH+D DG
Sbjct: 1   MAISTIAIPFKWFTKTNFKLNFPLLHSKHKP-ISSKPPTP-----PPLAEVAFRHLDIDG 60

Query: 61  DGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEKED 120
           DGKIS DELRSYF SIGE MT D A++VIGD+D DGDELLEL DFERL+K EEEE+E ED
Sbjct: 61  DGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQE-ED 120

Query: 121 LKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREFLR 180
           LK AFEMFE EKGCGFIG  GLQKMFGRLGYVKSKE+CM MI VFDVDGDGVIDY EFLR
Sbjct: 121 LKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLR 176

Query: 181 MMT 184
           MMT
Sbjct: 181 MMT 176

[Close]

BLAST of Lcy12g020460 vs. ExPASy TrEMBL
Match: A0A6J1CDM6 (probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC111010501 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.0e-67
Identity = 136/180 (75.56%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 3   ISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPPPLTAAPPFAEAAFRHIDTDGDG 62
           I T+SI  KWFSK+NFKLNFPRL    KP TSS PPPPPL AAPP AE AF H+D DGDG
Sbjct: 5   IPTVSIAFKWFSKSNFKLNFPRLLFNPKP-TSSNPPPPPLKAAPPLAEVAFHHLDADGDG 64

Query: 63  KISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEKEDLK 122
           KIS DELRSYF SIGE +T DD R VI D+DG+GDELLEL+DF RL++  +EEEE EDL+
Sbjct: 65  KISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEV-QEEEEGEDLR 124

Query: 123 MAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREFLRMM 182
            AFEMFEVEKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM
Sbjct: 125 RAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 182

[Close]

BLAST of Lcy12g020460 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 288.1 bits (736), Expect = 5.3e-74
Identity = 148/185 (80.00%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPP--PLTAAPPFAEAAFRHIDT 60
           MA+S IS P KWFS TNFKLNFPRL  KHKPT S+ PPPP   LT APP AEAAFRH+D 
Sbjct: 15  MALSPISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDA 74

Query: 61  DGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYF S+GE MT DDAR++I ++DGDGD LLEL DFERL+K   EEEEK
Sbjct: 75  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG-GEEEEK 134

Query: 121 EDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLKMAFEMFE EKGCGFIGP GLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 135 EDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 194

Query: 181 LRMMT 184
           LRMMT
Sbjct: 195 LRMMT 198

[Close]

BLAST of Lcy12g020460 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.1 bits (723), Expect = 1.7e-72
Identity = 146/185 (78.92%), Postives = 156/185 (84.32%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPP--PLTAAPPFAEAAFRHIDT 60
           MA+S IS P KWFS TN KLN PRL  KHKPT S+ PPPP   LT APP AEAAFRH+D 
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYF S+GE MT DDAR++I ++DGDGD LLEL DFERL+K   EEEEK
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG-GEEEEK 120

Query: 121 EDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLK AFEMFE EKGCGFIGPAGLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 121 EDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 180

Query: 181 LRMMT 184
           LRMMT
Sbjct: 181 LRMMT 184

[Close]

BLAST of Lcy12g020460 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 282.3 bits (721), Expect = 2.9e-72
Identity = 145/185 (78.38%), Postives = 156/185 (84.32%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPP--PLTAAPPFAEAAFRHIDT 60
           MA+S +S P KWFS TNF LNFPRL  KHKPT S+ PPPP   LT APP AEAAFRH+D 
Sbjct: 38  MALSPVSTPFKWFSNTNFNLNFPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 97

Query: 61  DGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYF S+GE MT DDAR++I ++D DGD LLEL DFERL+K   EEEEK
Sbjct: 98  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKG-GEEEEK 157

Query: 121 EDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLK AFEMFE EKGCGFIGPAGLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 158 EDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 217

Query: 181 LRMMT 184
           LRMMT
Sbjct: 218 LRMMT 221

[Close]

BLAST of Lcy12g020460 vs. NCBI nr
Match: KAG7024217.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 281.6 bits (719), Expect = 5.0e-72
Identity = 145/185 (78.38%), Postives = 156/185 (84.32%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPP--PLTAAPPFAEAAFRHIDT 60
           MA+S IS P KWFS TN KLN PRL  KHKPT S+ PPPP   LT APP AEAAFRH+D 
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYF S+GE MT D+AR++I ++DGDGD LLEL DFERL+K   EEEEK
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKG-GEEEEK 120

Query: 121 EDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLK AFEMFE EKGCGFIGPAGLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 121 EDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 180

Query: 181 LRMMT 184
           LRMMT
Sbjct: 181 LRMMT 184

[Close]

BLAST of Lcy12g020460 vs. NCBI nr
Match: XP_022936314.1 (probable calcium-binding protein CML41 [Cucurbita moschata])

HSP 1 Score: 280.4 bits (716), Expect = 1.1e-71
Identity = 144/184 (78.26%), Postives = 156/184 (84.78%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPP-PLTAAPPFAEAAFRHIDTD 60
           MA+S IS P KWFS TN KLN PRL  KHKPT S+ PPPP  LT APP AEAAFRH+D D
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLTPAPPLAEAAFRHLDAD 60

Query: 61  GDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLIKAEEEEEEKE 120
           GDGKIS DEL+SYF S+GE MT DDAR++I ++DGDGD LLEL DFERL+K   E+EEKE
Sbjct: 61  GDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG-GEQEEKE 120

Query: 121 DLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREFL 180
           DLK AFEMFE EKGCGFIGPAGLQKMFGRLGYVKS EECMAMIGVFDVDG+GVIDYREFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFL 180

Query: 181 RMMT 184
           RMMT
Sbjct: 181 RMMT 183

[Close]

BLAST of Lcy12g020460 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-27
Identity = 77/191 (40.31%), Postives = 104/191 (54.45%), Query Frame = 0


Query: 11  KWFSKTNFKLNFPRLHSKHKP-TTSSKPPPPPLT------------AAPPFAEAAFRHID 70
           KWFS    KLN    + +  P + SS     P +            A+       F H D
Sbjct: 14  KWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVFSHFD 73

Query: 71  TDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDF-----ERLIKAE 130
           +DGDGKIS+ ELR YF S+GE ++ + A+  I + D D D  L   DF      R +  +
Sbjct: 74  SDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRDLYGD 133

Query: 131 EEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGV 184
            E +   +LK AFEMFEVEKG G I P GLQKM  +LG  ++  EC AMI  +D+DG+G+
Sbjct: 134 GEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNGI 193

[Close]

BLAST of Lcy12g020460 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 101.3 bits (251), Expect = 8.6e-22
Identity = 50/133 (37.59%), Postives = 86/133 (64.66%), Query Frame = 0


Query: 50  EAAFRHIDTDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLI 109
           EA F ++D + DG+IS +EL+  F+++GE ++ ++A   +   D DGD +L+  +F +LI
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 110 KAEEEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDG 169
           K ++EEE+K +LK AF ++ + +G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 170 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Lcy12g020460 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 101.3 bits (251), Expect = 8.6e-22
Identity = 50/133 (37.59%), Postives = 86/133 (64.66%), Query Frame = 0


Query: 50  EAAFRHIDTDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLI 109
           EA F ++D + DG+IS +EL+  F+++GE ++ ++A   +   D DGD +L+  +F +LI
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 110 KAEEEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDG 169
           K ++EEE+K +LK AF ++ + +G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 170 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Lcy12g020460 vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 93.2 bits (230), Expect = 2.3e-19
Identity = 49/133 (36.84%), Postives = 81/133 (60.90%), Query Frame = 0


Query: 50  EAAFRHIDTDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLI 109
           EA F ++D + DG+IS +EL+  F+++GE +  D           DGD +L+  +F +LI
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLLSDT----------DGDGMLDFEEFSQLI 104

Query: 110 KAEEEEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFDVDG 169
           K ++EEE+K +LK AF ++ + +G   I P  L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 170 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 166

[Close]

BLAST of Lcy12g020460 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.0e-19
Identity = 50/136 (36.76%), Postives = 82/136 (60.29%), Query Frame = 0


Query: 50  EAAFRHIDTDGDGKISSDELRSYFVSIGESMTGDDARTVIGDYDGDGDELLELRDFERLI 109
           EA F ++D + DG+IS++EL+  F ++GE M+ ++A   +   D DGD +L++ +F  LI
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 110 KAEE---EEEEKEDLKMAFEMFEVEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIGVFD 169
           K  +   EEE+K  +  AF M+ +  G   I P  L+ M  +LG  ++ ++C  MI  FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMY-IADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 170 VDGDGVIDYREFLRMM 183
           ++ DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

The following BLAST results are available for this feature:

BLAST of Lcy12g020460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	3.3e-26	40.31	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q9SRE6	1.2e-20	37.59	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYK0	1.7e-19	42.47	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYJ8	1.5e-18	40.82	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE7	5.6e-18	36.76	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]

BLAST of Lcy12g020460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IIY2	2.6e-74	80.00	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A6J1F7Y7	5.4e-72	78.26	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7UHX6	2.7e-71	79.23	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	2.7e-71	79.23	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]
A0A6J1CDM6	1.0e-67	75.56	probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Lcy12g020460 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022974919.1	5.3e-74	80.00	probable calcium-binding protein CML41 [Cucurbita maxima]	[more]
KAG6591338.1	1.7e-72	78.92	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023535419.1	2.9e-72	78.38	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]
KAG7024217.1	5.0e-72	78.38	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022936314.1	1.1e-71	78.26	probable calcium-binding protein CML41 [Cucurbita moschata]	[more]

BLAST of Lcy12g020460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	2.3e-27	40.31	calmodulin-like 41	[more]
AT1G76650.1	8.6e-22	37.59	calmodulin-like 38	[more]
AT1G76650.3	8.6e-22	37.59	calmodulin-like 38	[more]
AT1G76650.2	2.3e-19	36.84	calmodulin-like 38	[more]
AT1G76640.1	4.0e-19	36.76	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 102..131
None	No IPR available	GENE3D	1.10.238.10		coord: 111..184 e-value: 2.5E-16 score: 61.5
None	No IPR available	GENE3D	1.10.238.10		coord: 18..110 e-value: 9.2E-15 score: 57.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 25..46
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 20..183
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 20..183
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 84..112 e-value: 29.0 score: 6.0 coord: 48..76 e-value: 2.9E-4 score: 30.2 coord: 157..184 e-value: 0.064 score: 22.4
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 157..183 e-value: 2.4E-7 score: 29.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 51..109 e-value: 2.0E-8 score: 34.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 153..184 score: 11.417016
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 44..79 score: 13.648647
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 120..183 e-value: 1.10711E-9 score: 50.2389
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 54..110 e-value: 5.33948E-9 score: 48.6981
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 57..69
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 166..178
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 51..182

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g020460.1	Lcy12g020460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity