Lcy12g020350 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g020350 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 9.1e-146
Identity = 269/529 (50.85%), Postives = 358/529 (67.67%), Query Frame = 0

Query: 9   MEIISPYLNSSSI--VGL-FLLLLFSVFVLKKGRAYKRRHPPEAAGGWPIVGHLPLLVGP 68
           ME++  +LN+++I  V L FLL LF    LKK      +  P   G WPI GHLPLL+G 
Sbjct: 1   MELVLHFLNTTTIGVVSLIFLLCLFLYGPLKKVHG-SSKEAPTVGGAWPIFGHLPLLIGS 60

Query: 69  KLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFS 128
           K PH+ LGA+A+K+GP+FTI++G  +A+V+S WE+A+EC+T  D  +S+RPK +  +L  
Sbjct: 61  KSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMC 120

Query: 129 YNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREG 188
           YN A     P Y  ++R +RKII +E+LS+ R+E  +DVRV EV+ S+ ELYDVW  R  
Sbjct: 121 YNNAMLLVAP-YGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVW--RSQ 180

Query: 189 GSESDDLVVDMDEWIGNINLNVILMMVCGKRF----CDDEEMRRCRRAMRDFFDLGGQFM 248
            +ESD   V++ +W      N++L MV GKRF      DE+  +C +A+ +F  L G F 
Sbjct: 181 KNESDYASVELKQWFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFT 240

Query: 249 VGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRELGK-ANGEHDFMGVMLS 308
           VGDAIP+LRWLD GG+EKA+K+T  ELD +I EWLEEHR++R LG+  +G  DFM VMLS
Sbjct: 241 VGDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLS 300

Query: 309 ILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDI 368
            L   G  + G DADT+ K+T + +I  GT+    T+ WA+ L+L N   L   + ELDI
Sbjct: 301 SL--DGKTIDGIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDI 360

Query: 369 HVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLIT 428
            VG  R + ESD+S LVYLQAVVKETLRLY  GPLS  REF++DC +GGY V  GTRLIT
Sbjct: 361 QVGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLIT 420

Query: 429 NLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQ 488
           N+WKI T+P VW++P EFKP+RFL++HK +DVKG +F+L PFG GRR CPG+ FG+Q   
Sbjct: 421 NIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVH 480

Query: 489 LVLASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAY 530
           L LAS +HS E+   S EP+DM    G+T  +  PL+VLVKP L  + Y
Sbjct: 481 LALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCY 522

BLAST of Lcy12g020350 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 510.4 bits (1313), Expect = 2.5e-143
Identity = 255/541 (47.13%), Postives = 356/541 (65.80%), Query Frame = 0

Query: 15  YLNSSSIVGLFLLLLFSVFVLKKGRAYKRRHPPEAAGGWPIVGHLPLLVGPKLPHETLGA 74
           YLN+++I  + L+ L   F+ +  +    + PP  +G WP++GHLPL+   + PH+TLGA
Sbjct: 5   YLNTTTIAFISLISLL-FFLFRFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGA 64

Query: 75  MADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFR 134
           + DKYGPIFTI++G   A+V+S+WE+AKEC+T  D V+SSRPK V  +L SYN A  G+ 
Sbjct: 65  LVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWA 124

Query: 135 PNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKR----------- 194
           P Y +++R +RKI+  E+LSNRR+EL   +RV EV+ S++EL +VW+ +           
Sbjct: 125 P-YGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDT 184

Query: 195 ------EGGSESDDLVVDMDEWIGNINLNVILMMVCGKRFCDD------EEMRRCRRAMR 254
                 +  S +D + V++ +W   + LN++L MV GKR   D      EE +R    +R
Sbjct: 185 KSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIR 244

Query: 255 DFFDLGGQFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRELG---KA 314
           DF  L G F VGD +PFL+WLDLGGHEK +KK   + D ++ EWLEEHR ++ LG   K 
Sbjct: 245 DFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKV 304

Query: 315 NGEHDFMGVMLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNR 374
            GE DFM  ML +L+++ ++  G+D DT+ KAT + LI GG+DT   TLTWA+ LLL + 
Sbjct: 305 VGERDFMDAMLLVLKDKPIE--GFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHP 364

Query: 375 EALRKAQEELDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVG 434
             L K +EEL+ ++G +R V+ESD++KLVYL A++KETLRLYP  P S  REF++DC +G
Sbjct: 365 HVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIG 424

Query: 435 GYDVAAGTRLITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRA 494
           GY +  GTRL+ NLWKI  +P VW +PLEFKPERFLS+HK +DV+GQNFEL PFG GRR 
Sbjct: 425 GYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRM 484

Query: 495 CPGLGFGVQMTQLVLASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATA 530
           C G+  G+ M   +LA+ +HS E+   S E +D+   L     +  PL+VLVKP L    
Sbjct: 485 CAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCLSFKC 541

BLAST of Lcy12g020350 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 2.7e-142
Identity = 248/515 (48.16%), Postives = 351/515 (68.16%), Query Frame = 0

Query: 16  LNSSSIVGLFLLLLFSVFVLKKGRAYKRRHPPEAAGGWPIVGHLPLLVGPKLPHETLGAM 75
           LN+ +I  +  L+   +F+ +K    + +  P  +G WPI+GHL LL G + PH+ LGA+
Sbjct: 10  LNTIAIASILSLIFLCLFLYRKNS--RGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGAL 69

Query: 76  ADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFRP 135
           ADKYGP+FTI++G+  A+V+S+WE++KE +T  D  +SSRPK V  ++ SYN A  G  P
Sbjct: 70  ADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAP 129

Query: 136 NYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGSESDDLVVD 195
            Y  ++R +RKI+  E LSNRR+E +  +RV EV+ S++EL+D+W+     +ES   +VD
Sbjct: 130 -YGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWS-NGNKNESRYTLVD 189

Query: 196 MDEWIGNINLNVILMMVCGKRFC------DDEEMRRCRRAMRDFFDLGGQFMVGDAIPFL 255
           + +W+  +  N+++ MV GKR+         ++ +R  + +R+F +L G F V D +P L
Sbjct: 190 IKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCL 249

Query: 256 RWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRELGK-ANGEHDFMGVMLSILQEQGMD 315
           RWLDLGGHEKA+K    E+D L+ EWLEEHR+++ LG+    + DFM VM+S L   G  
Sbjct: 250 RWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISAL--NGAQ 309

Query: 316 LAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHVGNKRLV 375
           +  +DADT+ KAT + LI GGTD+  VTLTWA+SLLL N  AL KA+EE+D+ +G    +
Sbjct: 310 IGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYI 369

Query: 376 DESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNLWKIQTE 435
            ESD+SKLVYLQA+VKETLRLYP  P S  REF+++C +GGY +  GTRLI NLWKI  +
Sbjct: 370 RESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRD 429

Query: 436 PRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLVLASVIH 495
           P VW++PLEFKPERFL++HK +D++G NFEL PFG GRR C G+  G+ M    LA+++H
Sbjct: 430 PSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLH 489

Query: 496 SLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPR 524
           S ++   S EPVDM    G T  +  PL++LVKPR
Sbjct: 490 SFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPR 518

BLAST of Lcy12g020350 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.4e-138
Identity = 250/514 (48.64%), Postives = 339/514 (65.95%), Query Frame = 0

Query: 21  IVGLFLLL-LFSVFVLKKGRAYKRRHPPEAAGGWPIVGHLPLLVGPKLPHETLGAMADKY 80
           ++G F+LL  F  FV KK    K + PP+ AG WPI+GHL LL    LPH+ L + ADK 
Sbjct: 11  LLGFFVLLPCFFYFVWKKPN-NKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKN 70

Query: 81  GPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFRPNYDS 140
           GP+F I++GVH+A+V+++ E+AKEC+T  D    +RP  V  K+  YN+   G  P Y  
Sbjct: 71  GPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAP-YGP 130

Query: 141 FYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGSESDDLVVDMDEW 200
           ++R +RKII  E LSNRRL+  + V   E+  S +ELY +W  +      D  +VDM +W
Sbjct: 131 YWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQ----NIDFCLVDMKQW 190

Query: 201 IGNINLNVILMMVCGKRF--------CDDEEMRRCRRAMRDFFDLGGQFMVGDAIPFLRW 260
           + ++ LN+ + MV GKRF        C++ E   C + +R+ F L G F++ D +P+LRW
Sbjct: 191 LADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRW 250

Query: 261 LDLGGHEKAIKKTLTELDCLIGEWLEEHRRRR-ELGKANGEHDFMGVMLSILQEQGMDLA 320
           LDLGGHEK +K+T+ ELD L   WL+EH+R+R   GK + + DFM VMLSIL+E  +   
Sbjct: 251 LDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEESKL--- 310

Query: 321 GYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHVGNKRLVDE 380
           G D DT+NK  C+A+I GG DT   TLTWA+SLLLNN  AL+KAQ+ELD+HVG  R VDE
Sbjct: 311 GNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDE 370

Query: 381 SDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNLWKIQTEPR 440
           SD+ KL Y+ A++KETLRLYP GPL G R  ++DC + GY V AGTRLI N WKIQ +P 
Sbjct: 371 SDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPL 430

Query: 441 VWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLVLASVIHSL 500
           VW++P E++PERFL   + +D+KGQ+FEL PFG GRRACP +   +Q+  L LA ++H  
Sbjct: 431 VWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGF 490

Query: 501 ELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRL 525
           EL T +   VDM    G+   +  PL+VLV PR+
Sbjct: 491 ELRTPNQNKVDMTETPGIVHAKATPLEVLVAPRI 513

BLAST of Lcy12g020350 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 5.4e-138
Identity = 253/520 (48.65%), Postives = 350/520 (67.31%), Query Frame = 0

Query: 19  SSIVGLFLLLLFSVFV--LKKGRAYKRRHPPEAAGGWPIVGHLPLLVG-PKLPHETLGAM 78
           +S+  LF+ +L  VF+   KK +  K    P  +G WPI+GHL LL G  +L + TLG M
Sbjct: 3   TSLFSLFVPILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 62

Query: 79  ADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFRP 138
           AD YGP  ++++G + A V+SS+EVAK+C+T  D  ++SRP +   K   YNFA FGF P
Sbjct: 63  ADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAP 122

Query: 139 NYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGSESDDLVVD 198
            Y +F+R +RKI   ELLSNRRL++ + VRV E+   V++LY +W K  G   +  ++VD
Sbjct: 123 -YSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGG---TKPVMVD 182

Query: 199 MDEWIGNINLNVILMMVCGKRFC---------DDEEMRRCRRAMRDFFDLGGQFMVGDAI 258
           +  W+ ++ LN+I+ MV GKR+          D EE  +C++A+  FF L G F V DA 
Sbjct: 183 LKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAF 242

Query: 259 PFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRREL-GKANGEHDFMGVMLSILQEQ 318
           P L + DL GHEK +K+T +ELD ++  W+E HR++R+  G    + DF+ VM+S+ ++ 
Sbjct: 243 PTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQG 302

Query: 319 GMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHVGNK 378
            +    YDA+T  K+TC+ALI GG+DT   TLTWAISLLLNN+E L+KAQ+E+DIHVG  
Sbjct: 303 KLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRD 362

Query: 379 RLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNLWKI 438
           R V++SD+  LVYLQA++KETLRLYPAGPL G RE  +DC V GY V  GTRLI N+WKI
Sbjct: 363 RNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKI 422

Query: 439 QTEPRVWAEPLEFKPERFLSSH-KHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLVLA 498
           Q +P+V+ EP EF+PERF++   K  DV+GQNFEL PFG GRR+CPG    +Q+  L LA
Sbjct: 423 QRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLA 482

Query: 499 SVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRL 525
             +HS ++ T  D PVDM+   GLT+ +  PL+VL+ PR+
Sbjct: 483 RFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Lcy12g020350 vs. ExPASy TrEMBL
Match: A0A6J1DRH5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111023576 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 3.2e-234
Identity = 407/529 (76.94%), Postives = 462/529 (87.33%), Query Frame = 0

Query: 8   SMEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHLPLLVGPK 67
           SMEIISPYLN++SI+ LFLLLL S  VLKK  +A KRR PP A G WPI+GHLPLL GP+
Sbjct: 9   SMEIISPYLNTNSIIPLFLLLLISYLVLKKLSKASKRRAPPAAGGAWPIIGHLPLLSGPQ 68

Query: 68  LPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSY 127
           LPHETLGAMADKYGPIFTI+VGVH  +VISSWEVAKECYT  DS++SSRPK+VGGKL  Y
Sbjct: 69  LPHETLGAMADKYGPIFTIQVGVHPTLVISSWEVAKECYTTHDSIVSSRPKTVGGKLLGY 128

Query: 128 NFAGFGFRPNYDSFYRSIRKIIASELLSNRRLEL--QRDVRVWEVKRSVRELYDVWTKRE 187
           NFAGFGFRP YDSFYRSIR+IIASE+LSNRRLEL  QRD+R  EVKRSVRELYD+W K+ 
Sbjct: 129 NFAGFGFRP-YDSFYRSIRRIIASEMLSNRRLELQFQRDIRDSEVKRSVRELYDLWEKKR 188

Query: 188 GGSESDDLV--VDMDEWIGNINLNVILMMVCGKRFCDDEEMRRCRRAMRDFFDLGGQFMV 247
             S+ DD+   VD+D W+G+++L VILMMVCGKRF  ++EM+RCRRAMR+FFDL GQFMV
Sbjct: 189 ETSDDDDVAVNVDLDGWVGSVDLKVILMMVCGKRFVGEKEMKRCRRAMREFFDLAGQFMV 248

Query: 248 GDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRELG-KANGEHDFMGVMLSI 307
           GDA+PFLRWLDLGG++KA+KKT  ELD ++ EWL+EHRR+ + G  A+G  DFM VMLS 
Sbjct: 249 GDALPFLRWLDLGGYQKAMKKTAKELDSIMEEWLDEHRRKADSGAAADGGPDFMDVMLST 308

Query: 308 LQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIH 367
           L  +G+DLAGYDA TVNKATCM +I+GGTDT T T+ WAISLLLNNREAL++AQEELDIH
Sbjct: 309 L--EGIDLAGYDAATVNKATCMTIIAGGTDTTTETILWAISLLLNNREALKRAQEELDIH 368

Query: 368 VGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITN 427
           VGNKRL+DESD+SKL YLQAVVKETLRLYPAGPLSGIREFS+DC VGGYDVA GTRLITN
Sbjct: 369 VGNKRLLDESDISKLEYLQAVVKETLRLYPAGPLSGIREFSRDCTVGGYDVAVGTRLITN 428

Query: 428 LWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQL 487
           LWKIQT+PRVWAEPL+FKPERFLSSHKHLDVKGQNFE  PFG GRR CPGLGFG+QMTQL
Sbjct: 429 LWKIQTDPRVWAEPLKFKPERFLSSHKHLDVKGQNFEFTPFGCGRRVCPGLGFGIQMTQL 488

Query: 488 VLASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAYA 531
           VLAS+IHS+EL TR DEPVDM A  G+TMHRVNPLQVL+KPRLLA+AYA
Sbjct: 489 VLASLIHSMELKTRFDEPVDMTADFGITMHRVNPLQVLIKPRLLASAYA 534

BLAST of Lcy12g020350 vs. ExPASy TrEMBL
Match: A0A6J1F9J8 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443336 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.7e-232
Identity = 412/536 (76.87%), Postives = 457/536 (85.26%), Query Frame = 0

Query: 1   MEFLKTKSMEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHL 60
           M+F +T SMEI+SP+LN   IVGL LLLLFSVF+LKK GR  K RHPPEA GGWPI+GHL
Sbjct: 1   MQFPETTSMEIVSPFLN---IVGLILLLLFSVFILKKLGRPCKGRHPPEADGGWPIIGHL 60

Query: 61  PLLVGPKLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSV 120
            LLVGPKLP+ETLG MADKYGPIFTIRVGV + +VIS+WEVAKECYT LDSV+SSRPKSV
Sbjct: 61  RLLVGPKLPYETLGDMADKYGPIFTIRVGVRKGLVISNWEVAKECYTTLDSVVSSRPKSV 120

Query: 121 GGKLFSYNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDV 180
            G+L SYNFAGFGFRP YDSFYRS+RKII S+LLSNRRL L RDVRV EVKR+V+ELYD+
Sbjct: 121 VGELLSYNFAGFGFRPYYDSFYRSVRKIIISKLLSNRRLALNRDVRVSEVKRAVKELYDL 180

Query: 181 WTKREGGSESDDLVVDMDEWIGNINLNVILMMVCGKRFC-DDEEMRRCRRAMRDFFDLGG 240
           W          DLVVDMDEWIGNINLNV LMMVCGKRF  DD  +RRCR+AMR+FFDLGG
Sbjct: 181 WITNR------DLVVDMDEWIGNINLNVSLMMVCGKRFIGDDAVVRRCRKAMREFFDLGG 240

Query: 241 QFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRE---LGKANGEHDFM 300
           QFMVGDAIPFLRWLDLGG+EKA+K T  ELDCLI +WLEEHRRRR       A+ EHDFM
Sbjct: 241 QFMVGDAIPFLRWLDLGGYEKAMKTTSRELDCLITDWLEEHRRRRHSSTAAAADSEHDFM 300

Query: 301 GVMLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQ 360
            VMLS L+E  MDLAGYDADTV KATCM LI+GGTDT+TVT+TWAIS LLNNRE LRKAQ
Sbjct: 301 DVMLSSLEEGTMDLAGYDADTVIKATCMTLIAGGTDTMTVTVTWAISSLLNNREILRKAQ 360

Query: 361 EELDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAG 420
           EELD+HVG +R+VDESD+SKLVYLQA+VKET RLYP GPL G+REFSQDC VGGY+VAAG
Sbjct: 361 EELDMHVGKERVVDESDISKLVYLQAIVKETFRLYPPGPLGGVREFSQDCTVGGYNVAAG 420

Query: 421 TRLITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFG 480
           TRLITNLWKIQT+PRVWAEPLEFKPERFL  HK LDV G+NFEL PFGYGRRACPGLG G
Sbjct: 421 TRLITNLWKIQTDPRVWAEPLEFKPERFLGRHKQLDVSGKNFELGPFGYGRRACPGLGIG 480

Query: 481 VQMTQLVLASVIHSLELGTRSDEPVDMAAC-LGLTMHRVNPLQVLVKPRLLATAYA 531
           VQMTQL+LA++IHS E+GTR DEPVDMAA   GLT  RV+ L++LVKPRL  +AYA
Sbjct: 481 VQMTQLLLATLIHSFEIGTRFDEPVDMAAVNSGLTARRVSSLKLLVKPRLPPSAYA 527

BLAST of Lcy12g020350 vs. ExPASy TrEMBL
Match: A0A6J1ILZ8 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476806 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 3.3e-231
Identity = 413/536 (77.05%), Postives = 453/536 (84.51%), Query Frame = 0

Query: 1   MEFLKTKSMEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHL 60
           M+F +T SMEIISP+LN   IVGL LL LFSVFVLKK GR  K RHPPEA GGWPI+GHL
Sbjct: 1   MQFPETTSMEIISPFLN---IVGLCLLFLFSVFVLKKLGRPCKGRHPPEADGGWPIIGHL 60

Query: 61  PLLVGPKLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSV 120
            LLVGPKLP+ETLG MADKYGPIFTIRVGV + +VISSWEVAKECYT LD VISSRP+SV
Sbjct: 61  RLLVGPKLPYETLGDMADKYGPIFTIRVGVRKGLVISSWEVAKECYTTLDPVISSRPESV 120

Query: 121 GGKLFSYNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDV 180
            G+L  YNFAGFGFRP YDSFYRS+RKII S+LLSN RL L RDVRV EVKR+V+ELYD+
Sbjct: 121 VGELLGYNFAGFGFRPYYDSFYRSVRKIIISKLLSNHRLALNRDVRVSEVKRAVKELYDL 180

Query: 181 WTKREGGSESDDLVVDMDEWIGNINLNVILMMVCGKRFC-DDEEMRRCRRAMRDFFDLGG 240
           W          DLVVDMDEWIGNINLN  LMMVCGKRF  DDE +RRCR+AMRDFFDLGG
Sbjct: 181 WITNR------DLVVDMDEWIGNINLNASLMMVCGKRFVGDDEVVRRCRKAMRDFFDLGG 240

Query: 241 QFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRE---LGKANGEHDFM 300
           QFMVGDAIPFLRWLDLGG+EKA+K T  ELDCLI EWLEEHRR+R       A+GEHDFM
Sbjct: 241 QFMVGDAIPFLRWLDLGGYEKAMKTTSRELDCLITEWLEEHRRKRHSSTAAAADGEHDFM 300

Query: 301 GVMLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQ 360
            VMLSIL+E  MDLAGYD DTV KATCM LI+GGTDT+TVT+TWAIS LLNNRE LRKAQ
Sbjct: 301 DVMLSILEEGTMDLAGYDTDTVIKATCMTLIAGGTDTMTVTVTWAISSLLNNREILRKAQ 360

Query: 361 EELDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAG 420
           EELD+HVG +R+VDESD+SKLVYLQA+VKET RLYP GPL G+REFSQDC VGGY+VAAG
Sbjct: 361 EELDMHVGKERVVDESDISKLVYLQAIVKETFRLYPPGPLGGVREFSQDCTVGGYNVAAG 420

Query: 421 TRLITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFG 480
           TRLITNL KIQT+PRVWAEPLEFKPERFL  HK LDV G+NFEL PFGYGRRACPGLG G
Sbjct: 421 TRLITNLRKIQTDPRVWAEPLEFKPERFLGRHKQLDVSGKNFELGPFGYGRRACPGLGIG 480

Query: 481 VQMTQLVLASVIHSLELGTRSDEPVDMAAC-LGLTMHRVNPLQVLVKPRLLATAYA 531
           VQMT L+LA++IHS E+GTR DEPVDMAA   GLT  RVN L++LVKPRLL +AYA
Sbjct: 481 VQMTHLLLATLIHSFEIGTRFDEPVDMAAVNSGLTARRVNSLKLLVKPRLLPSAYA 527

BLAST of Lcy12g020350 vs. ExPASy TrEMBL
Match: A0A6J1FAF2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443559 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.6e-217
Identity = 385/533 (72.23%), Postives = 438/533 (82.18%), Query Frame = 0

Query: 6   TKSMEIISPYLNSSSIVGLFLLLLFSVFVLKKGRAYKRR-HPPEAAGGWPIVGHLPLL-V 65
           T   + ISPYLNS+    LFLLLLFS FVLKK  + KRR  PPE AGGWPI+GHL LL  
Sbjct: 14  TSMADQISPYLNSTVAGLLFLLLLFSYFVLKKATSRKRRSQPPEVAGGWPIIGHLRLLST 73

Query: 66  GPKLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKL 125
             +L HETLG +ADKYGPIF IR+GV  A+VISSWEVA ECYT LDS++SSRPK +  K 
Sbjct: 74  DSQLLHETLGGLADKYGPIFGIRIGVRPALVISSWEVATECYTTLDSIVSSRPKIILQKK 133

Query: 126 FSYNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKR 185
             YNFAGFGFR + DSFYR++RKI+ASE LSNRRLELQRD+RV EVKRSV+E+Y +W K 
Sbjct: 134 LGYNFAGFGFRHHCDSFYRNMRKIVASEALSNRRLELQRDIRVSEVKRSVKEVYSLWAKT 193

Query: 186 EGGSESDDLVVDMDEWIGNINLNVILMMVCGKRF----CDDE-EMRRCRRAMRDFFDLGG 245
              S    LV+D+DEWIGNI LNVILMMVCGKRF    C DE EM RCR+AMR FFDLGG
Sbjct: 194 GEASSDSYLVLDLDEWIGNIALNVILMMVCGKRFVGGSCGDEREMERCRKAMRGFFDLGG 253

Query: 246 QFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRR-RRELGKANGEHDFMGV 305
           +F+VGDAIPFL+WLDLGG++KA  K   ELDCL+ EWLEEHRR +R+   A+GEHD M V
Sbjct: 254 KFIVGDAIPFLKWLDLGGYQKATNKVWKELDCLMEEWLEEHRRKKRDCSDADGEHDLMAV 313

Query: 306 MLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEE 365
           M S+L  +G+DLAGYDADTVNKATC+ LISGGTDT+ VTLTWAISLLLNN+EALRK +EE
Sbjct: 314 MPSLL--EGLDLAGYDADTVNKATCLTLISGGTDTMAVTLTWAISLLLNNQEALRKVEEE 373

Query: 366 LDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTR 425
           LDIHVG +R VDESD+SKLVYLQAV+KETLRLYPAGPLSG RE SQDC +GGY+VAAGT 
Sbjct: 374 LDIHVGKERAVDESDISKLVYLQAVIKETLRLYPAGPLSGAREVSQDCTIGGYNVAAGTY 433

Query: 426 LITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQ 485
           LITN+WKIQ +PR+W EP EFKPERFLSSHKH+DVKGQ+FEL+PFGYGRRACPG G  + 
Sbjct: 434 LITNIWKIQRDPRIWQEPSEFKPERFLSSHKHMDVKGQHFELSPFGYGRRACPGQGISLL 493

Query: 486 MTQLVLASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAYA 531
           MT LVLAS++HS +L TR DE VDM A LGLTMHRVNPLQVLVKPRLL TAYA
Sbjct: 494 MTPLVLASLVHSFQLRTRRDEAVDMTANLGLTMHRVNPLQVLVKPRLLPTAYA 544

BLAST of Lcy12g020350 vs. ExPASy TrEMBL
Match: A0A6J1FFB9 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443560 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 5.8e-212
Identity = 374/527 (70.97%), Postives = 434/527 (82.35%), Query Frame = 0

Query: 8   SMEIISPYLNSSSIVGLFLLLLFSVFVLKKGRAYKRRHPPEAAGGWPIVGHLPLL-VGPK 67
           S+ II PYLN + +  LFL LL S FV KK  A KRR PPE AGGWPI+GHLPLL    +
Sbjct: 7   SIGIIPPYLN-TFVAALFLFLLSSFFVFKKATAPKRREPPEVAGGWPIIGHLPLLKADSQ 66

Query: 68  LPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSY 127
           LPH+TLGA+ADKYGPIF IRVG    ++ISS E+A+EC++ LDS++ SRPKSVGGKL  Y
Sbjct: 67  LPHQTLGALADKYGPIFRIRVGAQPTLIISSSELARECHSTLDSIVCSRPKSVGGKLLGY 126

Query: 128 NFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGG 187
           N+A FG RP YDSFYRS+RKI+ SE+LSNR L L+RD+RV EVK+S++ELY+ WTKRE G
Sbjct: 127 NYAAFGARP-YDSFYRSMRKIVVSEVLSNRCLGLRRDIRVSEVKKSLKELYNQWTKREEG 186

Query: 188 SESDDLVVDMDEWIGNINLNVILMMVCGKRF----CDDEEMRRCRRAMRDFFDLGGQFMV 247
             SD ++VD+++WIGN+NL V+LMMVCGKRF     DDEEM+RCR+ MR+F DL G+F+V
Sbjct: 187 --SDHILVDIEQWIGNVNLRVVLMMVCGKRFLGDSADDEEMKRCRKVMREFLDLMGKFLV 246

Query: 248 GDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRELGKANGEHDFMGVMLSIL 307
           GD+IPFLRWLDLGG+EKA+K T  +LD L+ EWLEEHRR+R        +D M VMLS L
Sbjct: 247 GDSIPFLRWLDLGGYEKAMKITSKQLDSLLEEWLEEHRRKR-----TDHNDLMDVMLSNL 306

Query: 308 QEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHV 367
             +GMDLAGYDADTVNKATC+++I+GGTDT+TVTL+WAISLLLNNREALRK QEELDIHV
Sbjct: 307 --EGMDLAGYDADTVNKATCLSIITGGTDTVTVTLSWAISLLLNNREALRKTQEELDIHV 366

Query: 368 GNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNL 427
           G  R VDESD+SKLVYLQAVV ETLRLYP GPLSG+R FS+DC VGGYDV AGT LITNL
Sbjct: 367 GKDRQVDESDISKLVYLQAVVNETLRLYPPGPLSGVRVFSEDCTVGGYDVVAGTHLITNL 426

Query: 428 WKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLV 487
           WKIQT P+VWAEPLEFKPERF+S  K LDVKGQNFE APFG GRRACPG+  G+QMTQLV
Sbjct: 427 WKIQTNPQVWAEPLEFKPERFVSGEKQLDVKGQNFEFAPFGLGRRACPGMSLGIQMTQLV 486

Query: 488 LASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAY 530
           LAS+IHSLELGT SDEPVDMAA  GLTM+R NPLQVLVKPRL   AY
Sbjct: 487 LASLIHSLELGTGSDEPVDMAAGFGLTMYRANPLQVLVKPRLFTCAY 522

BLAST of Lcy12g020350 vs. NCBI nr
Match: XP_038896609.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 830.5 bits (2144), Expect = 8.3e-237
Identity = 415/528 (78.60%), Postives = 463/528 (87.69%), Query Frame = 0

Query: 9   MEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHLPLLVGPKL 68
           MEIISP+LN  ++ GLFL LL SVF LKK  RAYK R PP+AAGGWPI+GHL  L+GPKL
Sbjct: 1   MEIISPHLNLITL-GLFLFLLSSVFFLKKLTRAYKYRDPPQAAGGWPIIGHLRQLLGPKL 60

Query: 69  PHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYN 128
           PHETLGAMADKYGPIFTIRVGVH+A+VIS+WE AKECYT  DS+ISSRPKS+ G+L  YN
Sbjct: 61  PHETLGAMADKYGPIFTIRVGVHKALVISNWETAKECYTTHDSLISSRPKSLVGQLLGYN 120

Query: 129 FAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGS 188
           FAGFGFRPNYDSFYRSIRKIIASELLSNRRLE QR+VRV EVKR+V+E+Y++W ++    
Sbjct: 121 FAGFGFRPNYDSFYRSIRKIIASELLSNRRLEEQREVRVCEVKRAVKEVYELWNRK---- 180

Query: 189 ESDDLVVDMDEWIGNINLNVILMMVCGKR-FCDDEEMRRCRRAMRDFFDLGGQFMVGDAI 248
            ++DLV++MDEWIGN+NLNVILMMVCGKR F DDE MRRCR+ MR+FFDLGGQFMVGDAI
Sbjct: 181 -TEDLVLNMDEWIGNVNLNVILMMVCGKRLFGDDETMRRCRKVMREFFDLGGQFMVGDAI 240

Query: 249 PFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRE----LGKANGEHDFMGVMLSIL 308
           PFLRWLDLGG+EKA+K T  ELDC IGEWLEE RR R+       A+G  D M VMLSIL
Sbjct: 241 PFLRWLDLGGYEKAMKITSRELDCFIGEWLEEQRRNRDSATAAAAADGRRDLMDVMLSIL 300

Query: 309 QEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHV 368
            E+GM+L GYDADTV KATCMALI+GGTDT+TVTLTWAIS LLNNREALRK QEELDIHV
Sbjct: 301 -EEGMELGGYDADTVIKATCMALIAGGTDTVTVTLTWAISFLLNNREALRKTQEELDIHV 360

Query: 369 GNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNL 428
           GNKRLV+ESD++ LVYLQAVVKETLRLYPAGPLSGIREFSQDC +GGY VAAGTRLITNL
Sbjct: 361 GNKRLVNESDITNLVYLQAVVKETLRLYPAGPLSGIREFSQDCTLGGYHVAAGTRLITNL 420

Query: 429 WKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLV 488
           WKIQT+PRVW EPLEFKPERF+SS KH+DVKGQN+EL PFG+GRRACPGLGFGVQMTQLV
Sbjct: 421 WKIQTDPRVWVEPLEFKPERFVSSEKHVDVKGQNYELGPFGFGRRACPGLGFGVQMTQLV 480

Query: 489 LASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAYA 531
           LA ++HS E+GTR DEPVDM A  GLTMHRVNPLQV+VKPRLL TAYA
Sbjct: 481 LAILVHSFEIGTRFDEPVDMEANFGLTMHRVNPLQVIVKPRLLHTAYA 521

BLAST of Lcy12g020350 vs. NCBI nr
Match: XP_022156753.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 820.8 bits (2119), Expect = 6.6e-234
Identity = 407/529 (76.94%), Postives = 462/529 (87.33%), Query Frame = 0

Query: 8   SMEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHLPLLVGPK 67
           SMEIISPYLN++SI+ LFLLLL S  VLKK  +A KRR PP A G WPI+GHLPLL GP+
Sbjct: 9   SMEIISPYLNTNSIIPLFLLLLISYLVLKKLSKASKRRAPPAAGGAWPIIGHLPLLSGPQ 68

Query: 68  LPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSY 127
           LPHETLGAMADKYGPIFTI+VGVH  +VISSWEVAKECYT  DS++SSRPK+VGGKL  Y
Sbjct: 69  LPHETLGAMADKYGPIFTIQVGVHPTLVISSWEVAKECYTTHDSIVSSRPKTVGGKLLGY 128

Query: 128 NFAGFGFRPNYDSFYRSIRKIIASELLSNRRLEL--QRDVRVWEVKRSVRELYDVWTKRE 187
           NFAGFGFRP YDSFYRSIR+IIASE+LSNRRLEL  QRD+R  EVKRSVRELYD+W K+ 
Sbjct: 129 NFAGFGFRP-YDSFYRSIRRIIASEMLSNRRLELQFQRDIRDSEVKRSVRELYDLWEKKR 188

Query: 188 GGSESDDLV--VDMDEWIGNINLNVILMMVCGKRFCDDEEMRRCRRAMRDFFDLGGQFMV 247
             S+ DD+   VD+D W+G+++L VILMMVCGKRF  ++EM+RCRRAMR+FFDL GQFMV
Sbjct: 189 ETSDDDDVAVNVDLDGWVGSVDLKVILMMVCGKRFVGEKEMKRCRRAMREFFDLAGQFMV 248

Query: 248 GDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRELG-KANGEHDFMGVMLSI 307
           GDA+PFLRWLDLGG++KA+KKT  ELD ++ EWL+EHRR+ + G  A+G  DFM VMLS 
Sbjct: 249 GDALPFLRWLDLGGYQKAMKKTAKELDSIMEEWLDEHRRKADSGAAADGGPDFMDVMLST 308

Query: 308 LQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIH 367
           L  +G+DLAGYDA TVNKATCM +I+GGTDT T T+ WAISLLLNNREAL++AQEELDIH
Sbjct: 309 L--EGIDLAGYDAATVNKATCMTIIAGGTDTTTETILWAISLLLNNREALKRAQEELDIH 368

Query: 368 VGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITN 427
           VGNKRL+DESD+SKL YLQAVVKETLRLYPAGPLSGIREFS+DC VGGYDVA GTRLITN
Sbjct: 369 VGNKRLLDESDISKLEYLQAVVKETLRLYPAGPLSGIREFSRDCTVGGYDVAVGTRLITN 428

Query: 428 LWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQL 487
           LWKIQT+PRVWAEPL+FKPERFLSSHKHLDVKGQNFE  PFG GRR CPGLGFG+QMTQL
Sbjct: 429 LWKIQTDPRVWAEPLKFKPERFLSSHKHLDVKGQNFEFTPFGCGRRVCPGLGFGIQMTQL 488

Query: 488 VLASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAYA 531
           VLAS+IHS+EL TR DEPVDM A  G+TMHRVNPLQVL+KPRLLA+AYA
Sbjct: 489 VLASLIHSMELKTRFDEPVDMTADFGITMHRVNPLQVLIKPRLLASAYA 534

BLAST of Lcy12g020350 vs. NCBI nr
Match: XP_023535453.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 817.4 bits (2110), Expect = 7.2e-233
Identity = 414/536 (77.24%), Postives = 459/536 (85.63%), Query Frame = 0

Query: 1   MEFLKTKSMEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHL 60
           M+F +T SMEIISP+LN   IVGL LLLLFSVF+LKK GRA K RHPPEA GGWPI+GHL
Sbjct: 1   MQFPETTSMEIISPFLN---IVGLCLLLLFSVFILKKQGRACKGRHPPEADGGWPIIGHL 60

Query: 61  PLLVGPKLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSV 120
            LLVGPKLP+ETLG MADKYGPIFTIRVGV + +VIS+WEVAKECYT LDSV+SSRPKSV
Sbjct: 61  RLLVGPKLPYETLGDMADKYGPIFTIRVGVRKGLVISNWEVAKECYTTLDSVVSSRPKSV 120

Query: 121 GGKLFSYNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDV 180
            G+L SYNFAGFGFRP YDSFYRS+RKII S+LLSNRRL L RDVRV EVKR+++ELYD+
Sbjct: 121 VGELLSYNFAGFGFRPYYDSFYRSVRKIIISKLLSNRRLALNRDVRVSEVKRAMKELYDL 180

Query: 181 WTKREGGSESDDLVVDMDEWIGNINLNVILMMVCGKRFC-DDEEMRRCRRAMRDFFDLGG 240
           W          DLVVDMDEWIGNINLNV LMMVCGKRF  DD  +RRCR+AMR+FFDLGG
Sbjct: 181 WITNR------DLVVDMDEWIGNINLNVSLMMVCGKRFVGDDAVVRRCRKAMREFFDLGG 240

Query: 241 QFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRE---LGKANGEHDFM 300
           QFMVGDAIPFLRWLDLGG+EKA+K T  ELDCLI EWLEEHRR+R       A+ EHDFM
Sbjct: 241 QFMVGDAIPFLRWLDLGGYEKAMKTTSRELDCLITEWLEEHRRKRHSSTAAAADSEHDFM 300

Query: 301 GVMLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQ 360
            VMLSIL+E  MDLAGYDADTV KATCM LI+GGTDT+TVT+TWAIS LLNNRE LRKAQ
Sbjct: 301 DVMLSILEEGTMDLAGYDADTVIKATCMTLIAGGTDTMTVTVTWAISSLLNNREILRKAQ 360

Query: 361 EELDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAG 420
           EELD+HVG +R+VDESD+SKLVYLQA+VKET RLYP GPL G+REFSQDC VGGY+VAAG
Sbjct: 361 EELDMHVGKERVVDESDISKLVYLQAIVKETFRLYPPGPLGGVREFSQDCTVGGYNVAAG 420

Query: 421 TRLITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFG 480
           TRLITNLWKIQT+PRVWAEPLEFKPERFL   K LDV G+NFEL PFGYGRRACPGLG G
Sbjct: 421 TRLITNLWKIQTDPRVWAEPLEFKPERFLGRQKQLDVSGKNFELGPFGYGRRACPGLGIG 480

Query: 481 VQMTQLVLASVIHSLELGTRSDEPVDMAAC-LGLTMHRVNPLQVLVKPRLLATAYA 531
           VQMTQL+LA++IHS E+GTR DEPVDMAA   GLT  RV+ L++LVKPRLL +AYA
Sbjct: 481 VQMTQLLLATLIHSFEIGTRFDEPVDMAAVNSGLTARRVSSLKLLVKPRLLPSAYA 527

BLAST of Lcy12g020350 vs. NCBI nr
Match: XP_022936879.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 815.1 bits (2104), Expect = 3.6e-232
Identity = 412/536 (76.87%), Postives = 457/536 (85.26%), Query Frame = 0

Query: 1   MEFLKTKSMEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHL 60
           M+F +T SMEI+SP+LN   IVGL LLLLFSVF+LKK GR  K RHPPEA GGWPI+GHL
Sbjct: 1   MQFPETTSMEIVSPFLN---IVGLILLLLFSVFILKKLGRPCKGRHPPEADGGWPIIGHL 60

Query: 61  PLLVGPKLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSV 120
            LLVGPKLP+ETLG MADKYGPIFTIRVGV + +VIS+WEVAKECYT LDSV+SSRPKSV
Sbjct: 61  RLLVGPKLPYETLGDMADKYGPIFTIRVGVRKGLVISNWEVAKECYTTLDSVVSSRPKSV 120

Query: 121 GGKLFSYNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDV 180
            G+L SYNFAGFGFRP YDSFYRS+RKII S+LLSNRRL L RDVRV EVKR+V+ELYD+
Sbjct: 121 VGELLSYNFAGFGFRPYYDSFYRSVRKIIISKLLSNRRLALNRDVRVSEVKRAVKELYDL 180

Query: 181 WTKREGGSESDDLVVDMDEWIGNINLNVILMMVCGKRFC-DDEEMRRCRRAMRDFFDLGG 240
           W          DLVVDMDEWIGNINLNV LMMVCGKRF  DD  +RRCR+AMR+FFDLGG
Sbjct: 181 WITNR------DLVVDMDEWIGNINLNVSLMMVCGKRFIGDDAVVRRCRKAMREFFDLGG 240

Query: 241 QFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRE---LGKANGEHDFM 300
           QFMVGDAIPFLRWLDLGG+EKA+K T  ELDCLI +WLEEHRRRR       A+ EHDFM
Sbjct: 241 QFMVGDAIPFLRWLDLGGYEKAMKTTSRELDCLITDWLEEHRRRRHSSTAAAADSEHDFM 300

Query: 301 GVMLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQ 360
            VMLS L+E  MDLAGYDADTV KATCM LI+GGTDT+TVT+TWAIS LLNNRE LRKAQ
Sbjct: 301 DVMLSSLEEGTMDLAGYDADTVIKATCMTLIAGGTDTMTVTVTWAISSLLNNREILRKAQ 360

Query: 361 EELDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAG 420
           EELD+HVG +R+VDESD+SKLVYLQA+VKET RLYP GPL G+REFSQDC VGGY+VAAG
Sbjct: 361 EELDMHVGKERVVDESDISKLVYLQAIVKETFRLYPPGPLGGVREFSQDCTVGGYNVAAG 420

Query: 421 TRLITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFG 480
           TRLITNLWKIQT+PRVWAEPLEFKPERFL  HK LDV G+NFEL PFGYGRRACPGLG G
Sbjct: 421 TRLITNLWKIQTDPRVWAEPLEFKPERFLGRHKQLDVSGKNFELGPFGYGRRACPGLGIG 480

Query: 481 VQMTQLVLASVIHSLELGTRSDEPVDMAAC-LGLTMHRVNPLQVLVKPRLLATAYA 531
           VQMTQL+LA++IHS E+GTR DEPVDMAA   GLT  RV+ L++LVKPRL  +AYA
Sbjct: 481 VQMTQLLLATLIHSFEIGTRFDEPVDMAAVNSGLTARRVSSLKLLVKPRLPPSAYA 527

BLAST of Lcy12g020350 vs. NCBI nr
Match: XP_022976388.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 810.8 bits (2093), Expect = 6.8e-231
Identity = 413/536 (77.05%), Postives = 453/536 (84.51%), Query Frame = 0

Query: 1   MEFLKTKSMEIISPYLNSSSIVGLFLLLLFSVFVLKK-GRAYKRRHPPEAAGGWPIVGHL 60
           M+F +T SMEIISP+LN   IVGL LL LFSVFVLKK GR  K RHPPEA GGWPI+GHL
Sbjct: 1   MQFPETTSMEIISPFLN---IVGLCLLFLFSVFVLKKLGRPCKGRHPPEADGGWPIIGHL 60

Query: 61  PLLVGPKLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSV 120
            LLVGPKLP+ETLG MADKYGPIFTIRVGV + +VISSWEVAKECYT LD VISSRP+SV
Sbjct: 61  RLLVGPKLPYETLGDMADKYGPIFTIRVGVRKGLVISSWEVAKECYTTLDPVISSRPESV 120

Query: 121 GGKLFSYNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDV 180
            G+L  YNFAGFGFRP YDSFYRS+RKII S+LLSN RL L RDVRV EVKR+V+ELYD+
Sbjct: 121 VGELLGYNFAGFGFRPYYDSFYRSVRKIIISKLLSNHRLALNRDVRVSEVKRAVKELYDL 180

Query: 181 WTKREGGSESDDLVVDMDEWIGNINLNVILMMVCGKRFC-DDEEMRRCRRAMRDFFDLGG 240
           W          DLVVDMDEWIGNINLN  LMMVCGKRF  DDE +RRCR+AMRDFFDLGG
Sbjct: 181 WITNR------DLVVDMDEWIGNINLNASLMMVCGKRFVGDDEVVRRCRKAMRDFFDLGG 240

Query: 241 QFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRE---LGKANGEHDFM 300
           QFMVGDAIPFLRWLDLGG+EKA+K T  ELDCLI EWLEEHRR+R       A+GEHDFM
Sbjct: 241 QFMVGDAIPFLRWLDLGGYEKAMKTTSRELDCLITEWLEEHRRKRHSSTAAAADGEHDFM 300

Query: 301 GVMLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQ 360
            VMLSIL+E  MDLAGYD DTV KATCM LI+GGTDT+TVT+TWAIS LLNNRE LRKAQ
Sbjct: 301 DVMLSILEEGTMDLAGYDTDTVIKATCMTLIAGGTDTMTVTVTWAISSLLNNREILRKAQ 360

Query: 361 EELDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAG 420
           EELD+HVG +R+VDESD+SKLVYLQA+VKET RLYP GPL G+REFSQDC VGGY+VAAG
Sbjct: 361 EELDMHVGKERVVDESDISKLVYLQAIVKETFRLYPPGPLGGVREFSQDCTVGGYNVAAG 420

Query: 421 TRLITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFG 480
           TRLITNL KIQT+PRVWAEPLEFKPERFL  HK LDV G+NFEL PFGYGRRACPGLG G
Sbjct: 421 TRLITNLRKIQTDPRVWAEPLEFKPERFLGRHKQLDVSGKNFELGPFGYGRRACPGLGIG 480

Query: 481 VQMTQLVLASVIHSLELGTRSDEPVDMAAC-LGLTMHRVNPLQVLVKPRLLATAYA 531
           VQMT L+LA++IHS E+GTR DEPVDMAA   GLT  RVN L++LVKPRLL +AYA
Sbjct: 481 VQMTHLLLATLIHSFEIGTRFDEPVDMAAVNSGLTARRVNSLKLLVKPRLLPSAYA 527

BLAST of Lcy12g020350 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 492.7 bits (1267), Expect = 3.8e-139
Identity = 253/520 (48.65%), Postives = 350/520 (67.31%), Query Frame = 0

Query: 19  SSIVGLFLLLLFSVFV--LKKGRAYKRRHPPEAAGGWPIVGHLPLLVG-PKLPHETLGAM 78
           +S+  LF+ +L  VF+   KK +  K    P  +G WPI+GHL LL G  +L + TLG M
Sbjct: 3   TSLFSLFVPILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 62

Query: 79  ADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFRP 138
           AD YGP  ++++G + A V+SS+EVAK+C+T  D  ++SRP +   K   YNFA FGF P
Sbjct: 63  ADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAP 122

Query: 139 NYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGSESDDLVVD 198
            Y +F+R +RKI   ELLSNRRL++ + VRV E+   V++LY +W K  G   +  ++VD
Sbjct: 123 -YSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGG---TKPVMVD 182

Query: 199 MDEWIGNINLNVILMMVCGKRFC---------DDEEMRRCRRAMRDFFDLGGQFMVGDAI 258
           +  W+ ++ LN+I+ MV GKR+          D EE  +C++A+  FF L G F V DA 
Sbjct: 183 LKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAF 242

Query: 259 PFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRREL-GKANGEHDFMGVMLSILQEQ 318
           P L + DL GHEK +K+T +ELD ++  W+E HR++R+  G    + DF+ VM+S+ ++ 
Sbjct: 243 PTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQG 302

Query: 319 GMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHVGNK 378
            +    YDA+T  K+TC+ALI GG+DT   TLTWAISLLLNN+E L+KAQ+E+DIHVG  
Sbjct: 303 KLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRD 362

Query: 379 RLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNLWKI 438
           R V++SD+  LVYLQA++KETLRLYPAGPL G RE  +DC V GY V  GTRLI N+WKI
Sbjct: 363 RNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKI 422

Query: 439 QTEPRVWAEPLEFKPERFLSSH-KHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLVLA 498
           Q +P+V+ EP EF+PERF++   K  DV+GQNFEL PFG GRR+CPG    +Q+  L LA
Sbjct: 423 QRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLA 482

Query: 499 SVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRL 525
             +HS ++ T  D PVDM+   GLT+ +  PL+VL+ PR+
Sbjct: 483 RFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Lcy12g020350 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 485.0 bits (1247), Expect = 7.9e-137
Identity = 247/524 (47.14%), Postives = 350/524 (66.79%), Query Frame = 0

Query: 19  SSIVGLFLLLLFSVFV--LKKGRAYKRRHPPEAAGGWPIVGHLPLLVG-PKLPHETLGAM 78
           +S+  LF+ +L  VF+   KK +  K    P  +G WPI+GHL LL G  +L + TLG M
Sbjct: 3   TSLFSLFVPILVFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKM 62

Query: 79  ADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFRP 138
           AD+YGP  ++R+G     V+SS+EVAK+C+T  D  ++SRP +   K   Y+ A FGF P
Sbjct: 63  ADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAP 122

Query: 139 NYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGSESDDLVVD 198
            Y +F+R +RKI   ELLSNRRL++ + VRV E+   +++LY +W K+ G   S+ ++VD
Sbjct: 123 -YSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGG---SEPVMVD 182

Query: 199 MDEWIGNINLNVILMMVCGKRFC--------DDEEMRRCRRAMRDFFDLGGQFMVGDAIP 258
           +  W+ +++LN+++ MV GKR+         D EE R+CR+ + +FF L G F V DA P
Sbjct: 183 LKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFP 242

Query: 259 FLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRREL-GKANGEHDFMGVMLSILQEQG 318
            L W D  GHEK +K+T  ELD ++  W+E HR++R++ G  + + DF+ VMLS+ ++  
Sbjct: 243 KLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGK 302

Query: 319 MDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHVGNKR 378
                +DA T  K+TC+ALI GG++T   TLTWAISLLLNN++ L+KAQ+E+DIHVG  R
Sbjct: 303 FSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDR 362

Query: 379 LVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNLWKIQ 438
            V++SD+  LVY+QA++KETLRLYPAGPL G RE  +DC V GY+V  GTR++ N+WKIQ
Sbjct: 363 NVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQ 422

Query: 439 TEPRVWAEPLEFKPERFLSSH-KHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLVLAS 498
            +PRV+ EP EF+PERF++   K  DV+GQNFEL PFG GRR+CPG    +Q+  L LA 
Sbjct: 423 RDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLAR 482

Query: 499 VIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAY 530
            + S ++ T  D PVDM    GLT+ +  PL++L+ PRL    Y
Sbjct: 483 FLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of Lcy12g020350 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 456.1 bits (1172), Expect = 4.0e-128
Identity = 243/520 (46.73%), Postives = 334/520 (64.23%), Query Frame = 0

Query: 19  SSIVGLFLLLLFSVFV--LKKGRAYKRRHPPEAAGGWPIVGHLPLLVG-PKLPHETLGAM 78
           +S+  LF+ +L  VF+   KK +  K    P  +G WPI+GHL LL G  +L + TLG M
Sbjct: 3   TSLFSLFVSILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 62

Query: 79  ADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFRP 138
           AD YGP  ++R+G     V SS+EVAK+C+T  D  ++S   +   K   Y         
Sbjct: 63  ADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY--------- 122

Query: 139 NYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGSESDDLVVD 198
               F+  +RKI   ELLSNRRL++  +VRV E+   V++LY +W K+ G   S+ ++VD
Sbjct: 123 ---VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGG---SEPVMVD 182

Query: 199 MDEWIGNINLNVILMMVCGKRFC---------DDEEMRRCRRAMRDFFDLGGQFMVGDAI 258
           +  W+ ++  N+I+ MV GKR+            EE R+ R+ +  FF L G F V DA 
Sbjct: 183 LKSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAF 242

Query: 259 PFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRREL-GKANGEHDFMGVMLSILQEQ 318
           P L WLDL GHEK +K+T  ELD ++  W+E HR++R++ G  + + DF+ VMLS+ ++ 
Sbjct: 243 PKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQG 302

Query: 319 GMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHVGNK 378
            +    YDA+T  K TC+ALI GG++T   TLTWAISLLLNN++ L+K Q+E+DIHVG  
Sbjct: 303 KLSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRD 362

Query: 379 RLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVGGYDVAAGTRLITNLWKI 438
           R V++SD+  LVYLQA++KETLRLYPA PL G RE  +DC V GY+V  GTRLI N+WKI
Sbjct: 363 RNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKI 422

Query: 439 QTEPRVWAEPLEFKPERFLSSH-KHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLVLA 498
           Q +P+V+ EP EF+PERF++   K  DV+GQNFEL PFG GRR+CPG    +QM  L LA
Sbjct: 423 QRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLA 482

Query: 499 SVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRL 525
             +HS E+ T  D PVDM+   GLT+ +  PL+VL+ PRL
Sbjct: 483 RFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRL 506

BLAST of Lcy12g020350 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 367.5 bits (942), Expect = 1.8e-101
Identity = 207/523 (39.58%), Postives = 301/523 (57.55%), Query Frame = 0

Query: 21  IVGLFL---LLLFSVFVLKKGR---AYKRRHPPEAAGGWPIVGHLPLLVGPKLPHETLGA 80
           ++ LFL   L +F V +L K R     K+   P   G WP++GHL L       H T GA
Sbjct: 3   LIMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGA 62

Query: 81  MADKYGPIFTIRVGVHRAVVISSWEVAKECYTALDSVISSRPKSVGGKLFSYNFAGFGFR 140
           MAD YGP+F  ++G  + ++I+S EVAKE YT  D ++  RP+    KL  YN +   F 
Sbjct: 63  MADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFS 122

Query: 141 PNYDSFYRSIRKIIASELLSNRRLELQRDVRVWEVKRSVRELYDVWTKREGGSESDDLVV 200
           P Y  ++R IRKI  SEL S   +++    R  E   + R LY  W KR  G   + ++V
Sbjct: 123 P-YGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKR--GKPKEGVLV 182

Query: 201 DMDEWIGNINLNVILMMVCGKRF------CDDEEMRRCRRAMRDFFDLGGQFMVGDAIPF 260
           DM +   ++  N+ LMMV GKR+      C+ +E RRC + +R+F D    F++ D  P 
Sbjct: 183 DMKQEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPV 242

Query: 261 LRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRRELGKANGEHDFMGVMLSILQEQGMD 320
           L +LD    ++ +K+T   LD +   W+EEH+ +R       E+D++ +++ IL +  + 
Sbjct: 243 LGFLD-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRS-DHGRSENDYLDILIKILGQDKIP 302

Query: 321 LAGYDADTVNKATCMALISGGTDTITVTLTWAISLLLNNREALRKAQEELDIHVGNKRLV 380
               D  T  KA C+ L+  G++T  V L WA+SLLLNN   LRKAQEELD  +G +R+V
Sbjct: 303 GLS-DTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVV 362

Query: 381 DESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQDCRVG--GYDVAAGTRLITNLWKIQ 440
           +E D+  LVYLQA+VKET RLYP  PL   R   +D  +      V AGT+L+ + WKI 
Sbjct: 363 EELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIH 422

Query: 441 TEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFGYGRRACPGLGFGVQMTQLVLASV 500
            +P VW+ P +F+PERFL+S++ LDV GQ+++  PFG GRR+CP +  G++M   +L   
Sbjct: 423 RDPNVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRF 482

Query: 501 IHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPRLLATAY 530
           +HS +L   S + VDM    GL  H+  PL+V + PRL  + Y
Sbjct: 483 LHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLY 518

BLAST of Lcy12g020350 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 366.7 bits (940), Expect = 3.2e-101
Identity = 199/487 (40.86%), Postives = 289/487 (59.34%), Query Frame = 0

Query: 47  PEAAGGWPIVGHLPLLVGPKLPHETLGAMADKYGPIFTIRVGVHRAVVISSWEVAKECYT 106
           PE  G  P+ GHL LL G KL  + L AM+ K+GPIF++++G +R VV S  +  K+C+T
Sbjct: 40  PEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFT 99

Query: 107 ALDSVISSRPKSVGGKLFSYNFAGFGFRPNYDSFYRSIRKIIASELLSNRRLELQRDVRV 166
             D   ++RP    G+   YN A     P Y  ++R +RKI+   L SN  +E+   +R 
Sbjct: 100 TNDLATATRPNIAFGRYVGYNNASLTLAP-YGDYWRELRKIVTVHLFSNHSIEMLGHIRS 159

Query: 167 WEVKRSVRELYDVWTKREGGSESDDLVVDMDEWIGNINLNVILMMVCGKRFCDDE---EM 226
            EV   ++ LY    K  GG+     +V +D     +  N+IL  + GKR    E   + 
Sbjct: 160 SEVNTLIKHLY----KGNGGTS----IVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDE 219

Query: 227 RRCRRAMRDFFDLGGQFMVGDAIPFLRWLDLGGHEKAIKKTLTELDCLIGEWLEEHRRRR 286
            R + A++    L    M+GD IP+L WLD   + + +K+   ELD +  +WL EH ++R
Sbjct: 220 WRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAKNSQ-MKRLFKELDSVNTKWLHEHLKKR 279

Query: 287 ELGKANGEHDFMGVMLSILQEQGMDLAGYDADTVNKATCMALISGGTDTITVTLTWAISL 346
              + + E   M ++L IL E  + ++G+  D + KAT +AL   G+D+ ++TLTWA+SL
Sbjct: 280 SRNEKDQERTIMDLLLDILPED-IVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSL 339

Query: 347 LLNNREALRKAQEELDIHVGNKRLVDESDMSKLVYLQAVVKETLRLYPAGPLSGIREFSQ 406
           LLNN  AL  AQEE+D  VG  R ++ESD+  L YLQA+VKET RLYP  PL+GIRE  +
Sbjct: 340 LLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREARE 399

Query: 407 DCRVGGYDVAAGTRLITNLWKIQTEPRVWAEPLEFKPERFLSSHKHLDVKGQNFELAPFG 466
           DC VGGY V  GTRL+ N+WK+  +P++W +P  FKPERF+      +    NFE  PFG
Sbjct: 400 DCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFG 459

Query: 467 YGRRACPGLGFGVQMTQLVLASVIHSLELGTRSDEPVDMAACLGLTMHRVNPLQVLVKPR 526
            GRR+CPG+  G+++   VLA ++   EL   SDEP+DMA   GL + ++NP++V+V PR
Sbjct: 460 SGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPR 513

Query: 527 LLATAYA 531
           L    Y+
Sbjct: 520 LDPKLYS 513